E. coli BL21(DE3)
NEB ·
C2527I T7 RNA polymerase expression strain; standard host for IPTG-inducible protein production from pET-family vectors.
Bacterial strain
Protocols Using This Product (8)
- Alkaline Lysis Plasmid Miniprep (Birnboim & Doly method)
- Bst-LF Cellular Reagent Production (LAMP Isothermal Amplification)
- Heat-shock Transformation of Chemically Competent E. coli
- OpenTaq Cellular Reagent Production (Evaporative Drying)
- OpenVent Cellular Reagent Production (Evaporative Drying, High-Fidelity PCR)
- Preparing a Glycerol Stock for Long-term Strain Storage
- Preparing Chemically Competent E. coli by the CaCl2 Method
- RTX Reverse Transcriptase Cellular Reagent Production (One-Enzyme RT-PCR)