61 protocols available
Obtain high-molecular-weight genomic DNA suitable for long-read sequencing or Southern blotting. The protocol combines a...
To obtain 15-20 µg of high-quality, 20–150 kb genomic DNA suitable for downstream molecular biology applications from fr...
This procedure describes the steps for assessing the concentration and purity of nucleic acids (DNA or RNA) using a Nano...
A standardized method for preparing single and serial dilutions of DNA (e.g., 1:10, 1:100, 1:1000) for downstream assays...
To accurately quantify double‑stranded DNA (dsDNA) in the range of 0.2–100 ng total DNA per tube using the Qubit™ 1X dsD...
**Purpose** To size and quantify high‑molecular‑weight (HMW) genomic DNA (\~1.3 kb–165 kb) using the Agilent Femto Puls...
### Purpose To convert unmethylated cytosines in DNA to uracil (while preserving 5‑methylcytosine) using the EZ DNA M...
A **complete SOP** for generating **MiSeq‑ready bisulfite amplicon libraries** with **TruSeq‑tailed primers**. It assume...
This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114).
This is a column-based DNA extraction procedure that has a PCR inhibitor removal step.
Column-based approach to separately isolate DNA and RNA from the same sample.
Quick, spin-column purification of total RNA (including small/microRNAs) from a wide range of fungi species, susceptible...
This protocol describes how to carry out sequencing of cDNA using a reverse transcription and strand-switching method an...
Standardized procedure to isolate high-quality total RNA from animal tissues using silica spin columns.
Run total RNA on the Fragment Analyzer using the eukaryotic RNA analysis mode.
A validated procedure to generate amplified cDNA for PacBio Iso‑Seq from total RNA and optional size-binning of PCR‑ampl...
SMRTbell Express Template Prep v2.0 with adapter‑ligation barcodes (ALB)
Bind Sequel II polymerase to finished SMRTbell Iso‑Seq libraries, optionally pool after binding prior to sequencing on t...
Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library preparation from total RNA...
Adapter ligation library prep for nanopore sequencing using the legacy SQK-LSK109 kit.
Direct RNA nanopore sequencing library preparation using the SQK-RNA004 kit for native RNA strand analysis.
Simultaneous purification of genomic DNA and total RNA from the same tissue sample (≤30 mg) using selective AllPrep DNA ...
Quantification, normalisation, and equimolar pooling of individually barcoded Oxford Nanopore PCR-cDNA libraries prior t...
Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-473 kit. Suitable fo...
Size selection of DNA or cDNA libraries using AMPure XP or equivalent SPRI beads to enrich for fragments above a target ...
Nuclease wash of an Oxford Nanopore flow cell between sequencing runs using the Flow Cell Wash Kit (EXP-WSH004). Removes...
A thorough observation and documentation of your site's existing conditions before designing. Steps: 1. Walk the s...
Map the external energies that flow through your site — sun, wind, water, fire risk, noise, views, wildlife corridors. ...
Assign elements to permaculture zones (0-5) based on how frequently you need to visit or interact with them. Zones: - Z...
Design a plant guild — a community of mutually beneficial plants that support each other. A guild typically includes: -...
Plan the sequence of plantings and activities across seasons and years to build soil, establish plants, and develop the ...
Identifies teleost fish to species via the Folmer COI barcode region using the Ward et al. 2005 primer cocktail. Designe...
Standardised collection of retail fish/seafood tissue (sushi, sashimi, fillets) for downstream species-ID. Captures the ...
Produces a functional Taq DNA polymerase reagent without chromatographic purification, following the cellular-reagents a...
Standard bench procedure for introducing a plasmid DNA construct into chemically competent E. coli by brief heat-shock. ...
Cryopreservation method for bacterial strains using glycerol as a cryoprotectant, producing indefinitely-storable frozen...
Quantifying bacterial culture density by measuring optical density at 600 nm — the standard proxy for cell concentration...
Producing sterile agar plates with an antibiotic selection marker. The foundational solid-phase culture medium for selec...
Lyophilisation-free method for drying bacterial cell aliquots into room-temperature-stable reagent format, using a seale...
Produces a functional Vent DNA polymerase (Pyrococcus sp.) reagent as lyophilisation-free cellular reagent, using the sa...
Produces a functional Bst polymerase Large Fragment reagent (Bacillus stearothermophilus) as cellular reagent without pu...
Produces a functional RTX reverse transcriptase reagent as cellular reagent without purification. RTX is an engineered p...
Classic chemical miniprep for plasmid DNA from small-volume bacterial cultures, using alkaline lysis and differential pr...
Standard CaCl2-based preparation of chemically competent E. coli cells, suitable for heat-shock transformation with plas...
Denaturing polyacrylamide gel electrophoresis for separating proteins by molecular weight — the foundational protein QC ...
Fast, low-cost DNA extraction suitable for PCR-based species identification and similar downstream assays. Uses Chelex-1...
Foundational Polymerase Chain Reaction procedure for amplifying specific DNA sequences from a template. Covers master-mi...
Standard agarose gel electrophoresis for separating and visualising DNA by size. Uses SYBR Safe (blue-LED transilluminat...
Purification of PCR products (amplicons) using paramagnetic SPRI beads. Removes primers, primer-dimers, dNTPs, salts, an...
Preparation and submission of purified PCR products for outsourced Sanger sequencing. Covers concentration measurement, ...
Computational procedure for calling species from a Sanger-sequenced barcode read using NCBI BLAST + BOLD Systems. Covers...
Loop-mediated isothermal amplification (LAMP) workflow — uses four to six primers and a strand-displacing Bst polymerase...
Colorimetric readout of LAMP reactions by pH change. LAMP amplification releases protons as the polymerase extends prime...
Physical filtration of water samples through membrane filters to capture environmental DNA (cellular, extracellular, and...
Salt-precipitation DNA extraction from water-filter membranes preserved in Longmire buffer. Recovers environmental DNA s...
Kitchen-grade adaptation of lab-standard Chelex-100 DNA extraction (Walsh, Metzger & Higuchi 1991). Designed for cit...
High-molecular-weight genomic DNA extraction using cetyltrimethylammonium bromide (CTAB) lysis, chloroform phase separat...
Genomic DNA extraction using SDS lysis, high-salt protein precipitation, and isopropanol precipitation. No chloroform, n...
Pre-extraction step for honey-based DNA work: dissolves a honey sample in warm water, pellets the pollen fraction by low...
Fluorometric measurement of double-stranded DNA concentration using the Qubit dsDNA HS (High-Sensitivity) assay. Covers ...