Protocols

High-Salt CTAB DNA Extraction

Obtain high-molecular-weight genomic DNA suitable for long-read sequencing or Southern blotting. The protocol combines a...

Genomic-tip 20/G extraction of high-molecular-weight genomic DNA from ≤ 20 mg animal or plant tissue (with Proteinase K & RNase A treatments)

To obtain 15-20 µg of high-quality, 20–150 kb genomic DNA suitable for downstream molecular biology applications from fr...

Nanodrop analysis of nucleic acid concentration and purity

This procedure describes the steps for assessing the concentration and purity of nucleic acids (DNA or RNA) using a Nano...

Serial Dilution of DNA

A standardized method for preparing single and serial dilutions of DNA (e.g., 1:10, 1:100, 1:1000) for downstream assays...

Qubit™ 1X dsDNA HS Assay (High Sensitivity)

To accurately quantify double‑stranded DNA (dsDNA) in the range of 0.2–100 ng total DNA per tube using the Qubit™ 1X dsD...

Femto Pulse – Genomic DNA 165 kb (FP‑1002‑22, 22 cm)

**Purpose** To size and quantify high‑molecular‑weight (HMW) genomic DNA (\~1.3 kb–165 kb) using the Agilent Femto Puls...

EZ DNA Methylation‑Gold™ Kit — Bisulfite Conversion of DNA

### Purpose To convert unmethylated cytosines in DNA to uracil (while preserving 5‑methylcytosine) using the EZ DNA M...

Agarose Gel Electrophoresis for DNA Integrity/Size Assessment

## 1. Purpose To assess DNA integrity, approximate size range, and gross contamination by running samples on an agaro...

Bisulfite amplicon sequencing library preparation (using TruSeq adapters)

A **complete SOP** for generating **MiSeq‑ready bisulfite amplicon libraries** with **TruSeq‑tailed primers**. It assume...

Ligation sequencing DNA V14 (SQK-LSK114) library preparation and sequencing on MinION

This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114).

Loading and Running a Flow Cell on a Oxford Nanopore Technologies MinION

This SOP covers the steps from flow-cell insertion, priming, library loading, starting a sequencing run, and initial che...

DNA extraction of insect tissue using the ZymoBIONICS DNA miniprep kit

This is a column-based DNA extraction procedure that has a PCR inhibitor removal step.

DNA and RNA extraction from insects using the ZymoBIOMICS DNA/RNA miniprep kit

Column-based approach to separately isolate DNA and RNA from the same sample.

Extraction of RNA from yeast using the ZymoBIONICS YeaStar RNA kit

Quick, spin-column purification of total RNA (including small/microRNAs) from a wide range of fungi species, susceptible...

Direct cDNA sequencing V14 with SQK-LSK114

This protocol describes how to carry out sequencing of cDNA using a reverse transcription and strand-switching method an...

Total RNA extraction from animal tissues using PureLink RNA Mini Kit

Standardized procedure to isolate high-quality total RNA from animal tissues using silica spin columns.

Fragment Analyzer – RNA (Eukaryotic)

Run total RNA on the Fragment Analyzer using the eukaryotic RNA analysis mode.

PacBio Iso‑Seq cDNA Preparation — Reverse Transcription, Template Switching, and PCR Amplification and Bead-based Size Binning

A validated procedure to generate amplified cDNA for PacBio Iso‑Seq from total RNA and optional size-binning of PCR‑ampl...

Fragment Analyzer – HS Large Fragment 50Kb

PacBio Iso‑Seq Library Preparation — SMRTBell Ligation

SMRTbell Express Template Prep v2.0 with adapter‑ligation barcodes (ALB)

PacBio Sequel II — Polymerase Binding and Optional Post‑Binding Pooling

Bind Sequel II polymerase to finished SMRTbell Iso‑Seq libraries, optionally pool after binding prior to sequencing on t...

PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library preparation from total RNA...

Ligation sequencing DNA (SQK-LSK109) library preparation

Adapter ligation library prep for nanopore sequencing using the legacy SQK-LSK109 kit.

Direct RNA sequencing (SQK-RNA004) library preparation

Direct RNA nanopore sequencing library preparation using the SQK-RNA004 kit for native RNA strand analysis.

Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit

Simultaneous purification of genomic DNA and total RNA from the same tissue sample (≤30 mg) using selective AllPrep DNA ...

Equimolar pooling of barcoded ONT PCR-cDNA libraries

Quantification, normalisation, and equimolar pooling of individually barcoded Oxford Nanopore PCR-cDNA libraries prior t...

Fragment Analyzer – SS NGS Fragment 1-6000 bp

Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-473 kit. Suitable fo...

AMPure XP / SPRI Bead Size Selection

Size selection of DNA or cDNA libraries using AMPure XP or equivalent SPRI beads to enrich for fragments above a target ...

ONT Flow Cell Wash (EXP-WSH004)

Nuclease wash of an Oxford Nanopore flow cell between sequencing runs using the Flow Cell Wash Kit (EXP-WSH004). Removes...