Libre Biotech

Documentation

Protocols, training materials, instrument guides, and platform documentation.

Protocols & SOPs

Sample Preparation 20

High-Salt CTAB DNA Extraction
Obtain high-molecular-weight genomic DNA suitable for long-read sequencing or Southern blotting. The...
Genomic-tip 20/G extraction of high-molecular-weight genomic DNA from ≤ 20 mg animal or plant tissue (with Proteinase K & RNase A treatments)
To obtain 15-20 µg of high-quality, 20–150 kb genomic DNA suitable for downstream molecular biology ...
Serial Dilution of DNA
A standardized method for preparing single and serial dilutions of DNA (e.g., 1:10, 1:100, 1:1000) f...
EZ DNA Methylation‑Gold™ Kit — Bisulfite Conversion of DNA
### Purpose To convert unmethylated cytosines in DNA to uracil (while preserving 5‑methylcytosine...
Bisulfite amplicon sequencing library preparation (using TruSeq adapters)
A **complete SOP** for generating **MiSeq‑ready bisulfite amplicon libraries** with **TruSeq‑tailed ...
Ligation sequencing DNA V14 (SQK-LSK114) library preparation and sequencing on MinION
This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Ki...
DNA extraction of insect tissue using the ZymoBIONICS DNA miniprep kit
This is a column-based DNA extraction procedure that has a PCR inhibitor removal step.
DNA and RNA extraction from insects using the ZymoBIOMICS DNA/RNA miniprep kit
Column-based approach to separately isolate DNA and RNA from the same sample.
Extraction of RNA from yeast using the ZymoBIONICS YeaStar RNA kit
Quick, spin-column purification of total RNA (including small/microRNAs) from a wide range of fungi ...
Direct cDNA sequencing V14 with SQK-LSK114
This protocol describes how to carry out sequencing of cDNA using a reverse transcription and strand...
Total RNA extraction from animal tissues using PureLink RNA Mini Kit
Standardized procedure to isolate high-quality total RNA from animal tissues using silica spin colum...
PacBio Iso‑Seq cDNA Preparation — Reverse Transcription, Template Switching, and PCR Amplification and Bead-based Size Binning
A validated procedure to generate amplified cDNA for PacBio Iso‑Seq from total RNA and optional size...
PacBio Iso‑Seq Library Preparation — SMRTBell Ligation
SMRTbell Express Template Prep v2.0 with adapter‑ligation barcodes (ALB)
PacBio Sequel II — Polymerase Binding and Optional Post‑Binding Pooling
Bind Sequel II polymerase to finished SMRTbell Iso‑Seq libraries, optionally pool after binding prio...
PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)
Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library prepar...
Link
Ligation sequencing DNA (SQK-LSK109) library preparation
Adapter ligation library prep for nanopore sequencing using the legacy SQK-LSK109 kit.
Direct RNA sequencing (SQK-RNA004) library preparation
Direct RNA nanopore sequencing library preparation using the SQK-RNA004 kit for native RNA strand an...
Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit
Simultaneous purification of genomic DNA and total RNA from the same tissue sample (≤30 mg) using se...
Link
Equimolar pooling of barcoded ONT PCR-cDNA libraries
Quantification, normalisation, and equimolar pooling of individually barcoded Oxford Nanopore PCR-cD...
AMPure XP / SPRI Bead Size Selection
Size selection of DNA or cDNA libraries using AMPure XP or equivalent SPRI beads to enrich for fragm...
Link

Training Courses

Group management, user permissions, pipeline execution, JBrowse genome browser, API usage, advanced search, and administrative features for power users. [Preview content available - full course under development]

5 modules · 0 lessons

eDNA metabarcoding, specimen collection protocols, chain of custody tracking, and public data deposition. For museums, conservation groups, and field programs.

0 modules · 0 lessons

Dashboard setup, client reporting, quality control workflows, and onboarding delegation. For university core facilities and shared platforms.

3 modules · 9 lessons

Hands-on workshop transforming research projects from spreadsheets to FAIR science. Learn the ISA framework, metadata standards, provenance tracking, and export strategies.

5 modules · 9 lessons

Complete workflow from sample collection to publication. QC dashboard interpretation, CWL pipeline management, and JBrowse genome browser usage.

2 modules · 6 lessons

Complete beginner guide to the platform. Account setup, first investigation, sample upload, basic navigation, and understanding the interface. Perfect for new users. [Preview content available - full course under development]

5 modules · 0 lessons

Deep dive into Investigations, Studies, and Processes. How to structure research projects, sample lineage, metadata standards, and data organization best practices. [Preview content available - full course under development]

5 modules · 0 lessons

Create reproducible, shareable protocols using structured formats. Equipment & materials catalogs, version control, and collaborative improvement workflows.

2 modules · 4 lessons

Navigate the protocol library, follow procedures, create protocols, and interpret QC dashboards (IlluminaQC, NanoporeQC, PacBioQC). Quality control workflows. [Preview content available - full course under development]

5 modules · 0 lessons

Master sample tracking, batch operations, file attachments, process chains, provenance tracking, and metadata entry. Advanced data management workflows. [Preview content available - full course under development]

5 modules · 0 lessons

Technical guide for deploying Libre Biotech on your own infrastructure. Installation, configuration, administration, backups, and troubleshooting. [Preview content available - full course under development]

5 modules · 0 lessons

Platform Guide

How to use Libre Biotech for your lab work.

Getting Started
  • Create or join a Group to collaborate with your team
  • Start an Investigation to organize your research project
  • Add Studies under an investigation for different experimental arms
  • Create Processes (labbook entries) to record your bench work
ISA Framework
  • Investigation → top-level project container
  • Study → experimental design within an investigation
  • Assay / Process → individual lab procedures and data collection
  • Sample → biological or chemical material tracked through processes
Process Categories
  • Sample Preparation — extractions, library preps, pooling
  • Measurement — Fragment Analyzer, qPCR, spectrophotometry
  • Sequencing — sequencing submissions linked to QC runs
  • Data Transformation — computational analysis linked to pipelines
Collaboration
  • Groups control access — members see shared investigations and processes
  • Comments on processes and investigations for discussion
  • Reviews — request peer review and approval for processes
  • Sharing — create external links for collaborators outside the system