Documentation
Documentation
Protocols, training materials, instrument guides, and platform documentation.
Protocols & SOPs
Data Transformation 1
Design 5
A thorough observation and documentation of your site's existing conditions before designing. Steps...
Map the external energies that flow through your site — sun, wind, water, fire risk, noise, views, w...
Assign elements to permaculture zones (0-5) based on how frequently you need to visit or interact wi...
Design a plant guild — a community of mutually beneficial plants that support each other. A guild t...
Plan the sequence of plantings and activities across seasons and years to build soil, establish plan...
Measurement & QC 15
This procedure describes the steps for assessing the concentration and purity of nucleic acids (DNA ...
To accurately quantify double‑stranded DNA (dsDNA) in the range of 0.2–100 ng total DNA per tube usi...
**Purpose** To size and quantify high‑molecular‑weight (HMW) genomic DNA (\~1.3 kb–165 kb) using th...
Run total RNA on the Fragment Analyzer using the eukaryotic RNA analysis mode.
Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-...
Identifies teleost fish to species via the Folmer COI barcode region using the Ward et al. 2005 prim...
Quantifying bacterial culture density by measuring optical density at 600 nm — the standard proxy fo...
Denaturing polyacrylamide gel electrophoresis for separating proteins by molecular weight — the foun...
Foundational Polymerase Chain Reaction procedure for amplifying specific DNA sequences from a templa...
Standard agarose gel electrophoresis for separating and visualising DNA by size. Uses SYBR Safe (blu...
Preparation and submission of purified PCR products for outsourced Sanger sequencing. Covers concent...
Loop-mediated isothermal amplification (LAMP) workflow — uses four to six primers and a strand-displ...
Colorimetric readout of LAMP reactions by pH change. LAMP amplification releases protons as the poly...
Fluorometric measurement of double-stranded DNA concentration using the Qubit dsDNA HS (High-Sensiti...
Sample Preparation 39
Obtain high-molecular-weight genomic DNA suitable for long-read sequencing or Southern blotting. The...
To obtain 15-20 µg of high-quality, 20–150 kb genomic DNA suitable for downstream molecular biology ...
A standardized method for preparing single and serial dilutions of DNA (e.g., 1:10, 1:100, 1:1000) f...
### Purpose To convert unmethylated cytosines in DNA to uracil (while preserving 5‑methylcytosine...
A **complete SOP** for generating **MiSeq‑ready bisulfite amplicon libraries** with **TruSeq‑tailed ...
This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Ki...
This is a column-based DNA extraction procedure that has a PCR inhibitor removal step.
Column-based approach to separately isolate DNA and RNA from the same sample.
Quick, spin-column purification of total RNA (including small/microRNAs) from a wide range of fungi ...
This protocol describes how to carry out sequencing of cDNA using a reverse transcription and strand...
Standardized procedure to isolate high-quality total RNA from animal tissues using silica spin colum...
A validated procedure to generate amplified cDNA for PacBio Iso‑Seq from total RNA and optional size...
SMRTbell Express Template Prep v2.0 with adapter‑ligation barcodes (ALB)
Bind Sequel II polymerase to finished SMRTbell Iso‑Seq libraries, optionally pool after binding prio...
Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library prepar...
Adapter ligation library prep for nanopore sequencing using the legacy SQK-LSK109 kit.
Direct RNA nanopore sequencing library preparation using the SQK-RNA004 kit for native RNA strand an...
Simultaneous purification of genomic DNA and total RNA from the same tissue sample (≤30 mg) using se...
Quantification, normalisation, and equimolar pooling of individually barcoded Oxford Nanopore PCR-cD...
Size selection of DNA or cDNA libraries using AMPure XP or equivalent SPRI beads to enrich for fragm...
Standardised collection of retail fish/seafood tissue (sushi, sashimi, fillets) for downstream speci...
Produces a functional Taq DNA polymerase reagent without chromatographic purification, following the...
Standard bench procedure for introducing a plasmid DNA construct into chemically competent E. coli b...
Cryopreservation method for bacterial strains using glycerol as a cryoprotectant, producing indefini...
Producing sterile agar plates with an antibiotic selection marker. The foundational solid-phase cult...
Lyophilisation-free method for drying bacterial cell aliquots into room-temperature-stable reagent f...
Produces a functional Vent DNA polymerase (Pyrococcus sp.) reagent as lyophilisation-free cellular r...
Produces a functional Bst polymerase Large Fragment reagent (Bacillus stearothermophilus) as cellula...
Produces a functional RTX reverse transcriptase reagent as cellular reagent without purification. RT...
Classic chemical miniprep for plasmid DNA from small-volume bacterial cultures, using alkaline lysis...
Standard CaCl2-based preparation of chemically competent E. coli cells, suitable for heat-shock tran...
Fast, low-cost DNA extraction suitable for PCR-based species identification and similar downstream a...
Purification of PCR products (amplicons) using paramagnetic SPRI beads. Removes primers, primer-dime...
Physical filtration of water samples through membrane filters to capture environmental DNA (cellular...
Salt-precipitation DNA extraction from water-filter membranes preserved in Longmire buffer. Recovers...
Kitchen-grade adaptation of lab-standard Chelex-100 DNA extraction (Walsh, Metzger & Higuchi 1991). ...
High-molecular-weight genomic DNA extraction using cetyltrimethylammonium bromide (CTAB) lysis, chlo...
Genomic DNA extraction using SDS lysis, high-salt protein precipitation, and isopropanol precipitati...
Pre-extraction step for honey-based DNA work: dissolves a honey sample in warm water, pellets the po...
Training Courses
Advanced Features & Administration
Platform UsageGroup management, user permissions, pipeline execution, JBrowse genome browser, API usage, advanced search, and administrative features for power users. [Preview content available - full course under development]
Biodiversity & Environmental Genomics Workflows
Domain TrainingeDNA metabarcoding, specimen collection protocols, chain of custody tracking, and public data deposition. For museums, conservation groups, and field programs.
Core Facility Operations & Multi-Project Management
Institutional TrainingDashboard setup, client reporting, quality control workflows, and onboarding delegation. For university core facilities and shared platforms.
FAIR Data Management with Libre Biotech
Platform TrainingHands-on workshop transforming research projects from spreadsheets to FAIR science. Learn the ISA framework, metadata standards, provenance tracking, and export strategies.
Genomics Project Management End-to-End
Domain TrainingComplete workflow from sample collection to publication. QC dashboard interpretation, CWL pipeline management, and JBrowse genome browser usage.
Getting Started with Libre Biotech
Platform UsageComplete beginner guide to the platform. Account setup, first investigation, sample upload, basic navigation, and understanding the interface. Perfect for new users. [Preview content available - full course under development]
ISA Framework & Data Organization
Platform UsageDeep dive into Investigations, Studies, and Processes. How to structure research projects, sample lineage, metadata standards, and data organization best practices. [Preview content available - full course under development]
Protocol Development & Community Science
Platform TrainingCreate reproducible, shareable protocols using structured formats. Equipment & materials catalogs, version control, and collaborative improvement workflows.
Protocol Library & QC Dashboards
Platform UsageNavigate the protocol library, follow procedures, create protocols, and interpret QC dashboards (IlluminaQC, NanoporeQC, PacBioQC). Quality control workflows. [Preview content available - full course under development]
Sample & Process Management
Platform UsageMaster sample tracking, batch operations, file attachments, process chains, provenance tracking, and metadata entry. Advanced data management workflows. [Preview content available - full course under development]
Self-Hosting & Deployment
Platform UsageTechnical guide for deploying Libre Biotech on your own infrastructure. Installation, configuration, administration, backups, and troubleshooting. [Preview content available - full course under development]
Platform Guide
How to use Libre Biotech for your lab work.
Getting Started
- Create or join a Group to collaborate with your team
- Start an Investigation to organize your research project
- Add Studies under an investigation for different experimental arms
- Create Processes (labbook entries) to record your bench work
ISA Framework
- Investigation → top-level project container
- Study → experimental design within an investigation
- Assay / Process → individual lab procedures and data collection
- Sample → biological or chemical material tracked through processes
Process Categories
- Sample Preparation — extractions, library preps, pooling
- Measurement — Fragment Analyzer, qPCR, spectrophotometry
- Sequencing — sequencing submissions linked to QC runs
- Data Transformation — computational analysis linked to pipelines
Collaboration
- Groups control access — members see shared investigations and processes
- Comments on processes and investigations for discussion
- Reviews — request peer review and approval for processes
- Sharing — create external links for collaborators outside the system