Libre Biotech

Documentation

Protocols, training materials, instrument guides, and platform documentation.

Protocols & SOPs

Measurement & QC 15

Nanodrop analysis of nucleic acid concentration and purity
This procedure describes the steps for assessing the concentration and purity of nucleic acids (DNA ...
Qubit™ 1X dsDNA HS Assay (High Sensitivity)
To accurately quantify double‑stranded DNA (dsDNA) in the range of 0.2–100 ng total DNA per tube usi...
Femto Pulse – Genomic DNA 165 kb (FP‑1002‑22, 22 cm)
**Purpose** To size and quantify high‑molecular‑weight (HMW) genomic DNA (\~1.3 kb–165 kb) using th...
Fragment Analyzer – RNA (Eukaryotic)
Run total RNA on the Fragment Analyzer using the eukaryotic RNA analysis mode.
Fragment Analyzer – HS Large Fragment 50Kb
Fragment Analyzer – SS NGS Fragment 1-6000 bp
Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-...
Link
COI DNA Barcoding for Consumer Fish Samples
Identifies teleost fish to species via the Folmer COI barcode region using the Ward et al. 2005 prim...
Link
OD600 Measurement by Spectrophotometer
Quantifying bacterial culture density by measuring optical density at 600 nm — the standard proxy fo...
SDS-PAGE Gel Preparation and Running (Laemmli discontinuous buffer system)
Denaturing polyacrylamide gel electrophoresis for separating proteins by molecular weight — the foun...
Standard PCR Setup and Thermocycling
Foundational Polymerase Chain Reaction procedure for amplifying specific DNA sequences from a templa...
Agarose Gel Electrophoresis for DNA Visualisation
Standard agarose gel electrophoresis for separating and visualising DNA by size. Uses SYBR Safe (blu...
Sanger Sequencing Sample Submission (Outsourced)
Preparation and submission of purified PCR products for outsourced Sanger sequencing. Covers concent...
LAMP Reaction Setup and Thermal Incubation
Loop-mediated isothermal amplification (LAMP) workflow — uses four to six primers and a strand-displ...
LAMP Visual Readout by pH Indicator (Hydroxynaphthol Blue)
Colorimetric readout of LAMP reactions by pH change. LAMP amplification releases protons as the poly...
Fluorometric dsDNA Quantification (Qubit dsDNA HS Assay)
Fluorometric measurement of double-stranded DNA concentration using the Qubit dsDNA HS (High-Sensiti...

Sample Preparation 39

High-Salt CTAB DNA Extraction
Obtain high-molecular-weight genomic DNA suitable for long-read sequencing or Southern blotting. The...
Genomic-tip 20/G extraction of high-molecular-weight genomic DNA from ≤ 20 mg animal or plant tissue (with Proteinase K & RNase A treatments)
To obtain 15-20 µg of high-quality, 20–150 kb genomic DNA suitable for downstream molecular biology ...
Serial Dilution of DNA
A standardized method for preparing single and serial dilutions of DNA (e.g., 1:10, 1:100, 1:1000) f...
EZ DNA Methylation‑Gold™ Kit — Bisulfite Conversion of DNA
### Purpose To convert unmethylated cytosines in DNA to uracil (while preserving 5‑methylcytosine...
Bisulfite amplicon sequencing library preparation (using TruSeq adapters)
A **complete SOP** for generating **MiSeq‑ready bisulfite amplicon libraries** with **TruSeq‑tailed ...
Ligation sequencing DNA V14 (SQK-LSK114) library preparation and sequencing on MinION
This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Ki...
DNA extraction of insect tissue using the ZymoBIONICS DNA miniprep kit
This is a column-based DNA extraction procedure that has a PCR inhibitor removal step.
DNA and RNA extraction from insects using the ZymoBIOMICS DNA/RNA miniprep kit
Column-based approach to separately isolate DNA and RNA from the same sample.
Extraction of RNA from yeast using the ZymoBIONICS YeaStar RNA kit
Quick, spin-column purification of total RNA (including small/microRNAs) from a wide range of fungi ...
Direct cDNA sequencing V14 with SQK-LSK114
This protocol describes how to carry out sequencing of cDNA using a reverse transcription and strand...
Total RNA extraction from animal tissues using PureLink RNA Mini Kit
Standardized procedure to isolate high-quality total RNA from animal tissues using silica spin colum...
PacBio Iso‑Seq cDNA Preparation — Reverse Transcription, Template Switching, and PCR Amplification and Bead-based Size Binning
A validated procedure to generate amplified cDNA for PacBio Iso‑Seq from total RNA and optional size...
PacBio Iso‑Seq Library Preparation — SMRTBell Ligation
SMRTbell Express Template Prep v2.0 with adapter‑ligation barcodes (ALB)
PacBio Sequel II — Polymerase Binding and Optional Post‑Binding Pooling
Bind Sequel II polymerase to finished SMRTbell Iso‑Seq libraries, optionally pool after binding prio...
PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)
Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library prepar...
Link
Ligation sequencing DNA (SQK-LSK109) library preparation
Adapter ligation library prep for nanopore sequencing using the legacy SQK-LSK109 kit.
Direct RNA sequencing (SQK-RNA004) library preparation
Direct RNA nanopore sequencing library preparation using the SQK-RNA004 kit for native RNA strand an...
Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit
Simultaneous purification of genomic DNA and total RNA from the same tissue sample (≤30 mg) using se...
Link
Equimolar pooling of barcoded ONT PCR-cDNA libraries
Quantification, normalisation, and equimolar pooling of individually barcoded Oxford Nanopore PCR-cD...
AMPure XP / SPRI Bead Size Selection
Size selection of DNA or cDNA libraries using AMPure XP or equivalent SPRI beads to enrich for fragm...
Link
Retail tissue collection for mislabeling studies
Standardised collection of retail fish/seafood tissue (sushi, sashimi, fillets) for downstream speci...
Link
OpenTaq Cellular Reagent Production (Evaporative Drying)
Produces a functional Taq DNA polymerase reagent without chromatographic purification, following the...
Link
Heat-shock Transformation of Chemically Competent E. coli
Standard bench procedure for introducing a plasmid DNA construct into chemically competent E. coli b...
Preparing a Glycerol Stock for Long-term Strain Storage
Cryopreservation method for bacterial strains using glycerol as a cryoprotectant, producing indefini...
Preparing LB Agar Plates with Antibiotic Selection
Producing sterile agar plates with an antibiotic selection marker. The foundational solid-phase cult...
Evaporative Drying in a Desiccator Jar (for Cellular Reagents)
Lyophilisation-free method for drying bacterial cell aliquots into room-temperature-stable reagent f...
Link
OpenVent Cellular Reagent Production (Evaporative Drying, High-Fidelity PCR)
Produces a functional Vent DNA polymerase (Pyrococcus sp.) reagent as lyophilisation-free cellular r...
Link
Bst-LF Cellular Reagent Production (LAMP Isothermal Amplification)
Produces a functional Bst polymerase Large Fragment reagent (Bacillus stearothermophilus) as cellula...
Link
RTX Reverse Transcriptase Cellular Reagent Production (One-Enzyme RT-PCR)
Produces a functional RTX reverse transcriptase reagent as cellular reagent without purification. RT...
Link
Alkaline Lysis Plasmid Miniprep (Birnboim & Doly method)
Classic chemical miniprep for plasmid DNA from small-volume bacterial cultures, using alkaline lysis...
Preparing Chemically Competent E. coli by the CaCl2 Method
Standard CaCl2-based preparation of chemically competent E. coli cells, suitable for heat-shock tran...
Chelex-100 DNA Extraction for PCR-Ready Template
Fast, low-cost DNA extraction suitable for PCR-based species identification and similar downstream a...
PCR Cleanup by SPRI (Solid-Phase Reversible Immobilisation) Beads
Purification of PCR products (amplicons) using paramagnetic SPRI beads. Removes primers, primer-dime...
eDNA Water Filtration for Biodiversity Surveys
Physical filtration of water samples through membrane filters to capture environmental DNA (cellular...
eDNA Extraction from Filter Membranes (Salt-Precipitation Method)
Salt-precipitation DNA extraction from water-filter membranes preserved in Longmire buffer. Recovers...
Chelex DNA Extraction for Citizen Submitters (Kitchen Edition)
Kitchen-grade adaptation of lab-standard Chelex-100 DNA extraction (Walsh, Metzger & Higuchi 1991). ...
Link
CTAB DNA Extraction (Cross-Tissue Reference Protocol)
High-molecular-weight genomic DNA extraction using cetyltrimethylammonium bromide (CTAB) lysis, chlo...
SDS + Salt-Precipitation DNA Extraction (Consumer-Safe Cross-Tissue Protocol)
Genomic DNA extraction using SDS lysis, high-salt protein precipitation, and isopropanol precipitati...
Pollen Recovery from Honey (Pre-Extraction Concentration Step)
Pre-extraction step for honey-based DNA work: dissolves a honey sample in warm water, pellets the po...

Training Courses

Group management, user permissions, pipeline execution, JBrowse genome browser, API usage, advanced search, and administrative features for power users. [Preview content available - full course under development]

5 modules · 0 lessons

eDNA metabarcoding, specimen collection protocols, chain of custody tracking, and public data deposition. For museums, conservation groups, and field programs.

0 modules · 0 lessons

Dashboard setup, client reporting, quality control workflows, and onboarding delegation. For university core facilities and shared platforms.

3 modules · 9 lessons

Hands-on workshop transforming research projects from spreadsheets to FAIR science. Learn the ISA framework, metadata standards, provenance tracking, and export strategies.

5 modules · 9 lessons

Complete workflow from sample collection to publication. QC dashboard interpretation, CWL pipeline management, and JBrowse genome browser usage.

2 modules · 6 lessons

Complete beginner guide to the platform. Account setup, first investigation, sample upload, basic navigation, and understanding the interface. Perfect for new users. [Preview content available - full course under development]

5 modules · 0 lessons

Deep dive into Investigations, Studies, and Processes. How to structure research projects, sample lineage, metadata standards, and data organization best practices. [Preview content available - full course under development]

5 modules · 0 lessons

Create reproducible, shareable protocols using structured formats. Equipment & materials catalogs, version control, and collaborative improvement workflows.

2 modules · 4 lessons

Navigate the protocol library, follow procedures, create protocols, and interpret QC dashboards (IlluminaQC, NanoporeQC, PacBioQC). Quality control workflows. [Preview content available - full course under development]

5 modules · 0 lessons

Master sample tracking, batch operations, file attachments, process chains, provenance tracking, and metadata entry. Advanced data management workflows. [Preview content available - full course under development]

5 modules · 0 lessons

Technical guide for deploying Libre Biotech on your own infrastructure. Installation, configuration, administration, backups, and troubleshooting. [Preview content available - full course under development]

5 modules · 0 lessons

Platform Guide

How to use Libre Biotech for your lab work.

Getting Started
  • Create or join a Group to collaborate with your team
  • Start an Investigation to organize your research project
  • Add Studies under an investigation for different experimental arms
  • Create Processes (labbook entries) to record your bench work
ISA Framework
  • Investigation → top-level project container
  • Study → experimental design within an investigation
  • Assay / Process → individual lab procedures and data collection
  • Sample → biological or chemical material tracked through processes
Process Categories
  • Sample Preparation — extractions, library preps, pooling
  • Measurement — Fragment Analyzer, qPCR, spectrophotometry
  • Sequencing — sequencing submissions linked to QC runs
  • Data Transformation — computational analysis linked to pipelines
Collaboration
  • Groups control access — members see shared investigations and processes
  • Comments on processes and investigations for discussion
  • Reviews — request peer review and approval for processes
  • Sharing — create external links for collaborators outside the system