SMRTbell Express Template Prep Kit 2.0 Manual SDS
PacBio ·
100-938-900 · 18 reactions Library preparation with overhang SMRTbell adapter. Store frozen between -15 to -25°C.
Library preparation kit
Kit Components (14)
| Component | Type | Catalog # | Quantity | Storage | Notes |
|---|---|---|---|---|---|
| 10X primer buffer v2 | Buffer | 001-560-849 |
1 mL | -15 to -25°C | |
| 1x elution buffer | Elution buffer | 100-159-800 |
20 µL | -15 to -25°C | |
| DNA damage repair mix v2 | Library prep reagent | 101-653-800 |
18 µL | -15 to -25°C | |
| DNA prep additive | Library prep reagent | 101-653-600 |
1.5 mL | -15 to -25°C | |
| DNA prep buffer | Buffer | 101-653-300 |
18 µL | -15 to -25°C | |
| DNA prep enzyme | Library prep reagent | 101-653-700 |
18 µL | -15 to -25°C | |
| End prep mix | Library prep reagent | 101-653-900 |
540 µL | -15 to -25°C | |
| Enzyme dilution buffer | Buffer | 101-653-500 |
20 µL | -15 to -25°C | |
| Ligation additive | Ligation reagent | 101-654-200 |
41 µL | -15 to -25°C | |
| Ligation enhancer | Ligation reagent | 101-654-300 |
20 µL | -15 to -25°C | |
| Ligation mix | Ligation reagent | 101-654-100 |
60 µL | -15 to -25°C | |
| NAD | Buffer | 101-653-400 |
140 µL | -15 to -25°C | |
| Overhang adapter v3 | Sequencing adapter | 101-654-000 |
90 µL | -15 to -25°C | |
| Sequencing primer v4 | Sequencing primer | 101-654-600 |
110 µL | -15 to -25°C |
Protocols Using This Product (1)