Simultaneous extraction of DNA and RNA from C57 and DBA cortex tissue using Qiagen AllPrep

Status

In Progress

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Files

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Team

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Used in Studies

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Process Details

Process ID: #111
Category: Sample_prep
Process Date: November 17, 2016
Created: February 22, 2026 pm 21:08
Locked: No

Team 1

Kevin Dudley (Operator)

Used in 1 research study:

Strain-specific cortex gene expression and isoform usage (C57BL/6 and DBA/2 Mouse Cortex Transcriptomics)

Created Samples 16

1-D
C57 DNA
1-R
C57 RNA
10-D
DBA DNA
10-R
DBA RNA
2-D
C57 DNA
2-R
C57 RNA
3-D
C57 DNA
3-R
C57 RNA
4-D
C57 DNA
4-R
C57 RNA
7-D
DBA DNA
7-R
DBA RNA
8-D
DBA DNA
8-R
DBA RNA
9-D
DBA DNA
9-R
DBA RNA

Source Samples 8

1-C57-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
1-DBA-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
2-C57-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
2-DBA-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
3-C57-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
3-DBA-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
4-C57-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued
4-DBA-CORTEX
Origin: Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

1-D

Sample #397

Source Samples (Parents) 1

1-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 1-C57-CORTEX L1 via Process #115
└─ C57-1 L2 via Process #113

1-R

Sample #405

Source Samples (Parents) 1

1-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 1

C57_rep1_bc19
via Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)

All Ancestors 2

└─ 1-C57-CORTEX L1 via Process #115
└─ C57-1 L2 via Process #113

All Descendants 6

└─ C57_rep1_bc19 L1 via Process #97
└─ C57_DBA_pool_run1 L2 via Process #119
└─ C57_rep1_bc10 L2 via Process #121
└─ C57_rep1_bc19_1.5kb L2 via Process #118
└─ C57_DBA_pool_25fmol L3 via Process #116
└─ C57_DBA_pool_run3 L3 via Process #122

10-D

Sample #404

Source Samples (Parents) 1

4-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 4-DBA-CORTEX L1 via Process #115
└─ DBA-4 L2 via Process #113

10-R

Sample #412

Source Samples (Parents) 1

4-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 4-DBA-CORTEX L1 via Process #115
└─ DBA-4 L2 via Process #113

2-D

Sample #398

Source Samples (Parents) 1

2-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 2-C57-CORTEX L1 via Process #115
└─ C57-2 L2 via Process #113

2-R

Sample #406

Source Samples (Parents) 1

2-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 1

C57_rep2_bc20
via Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)

All Ancestors 2

└─ 2-C57-CORTEX L1 via Process #115
└─ C57-2 L2 via Process #113

All Descendants 6

└─ C57_rep2_bc20 L1 via Process #97
└─ C57_DBA_pool_run1 L2 via Process #119
└─ C57_rep2_bc11 L2 via Process #121
└─ C57_rep2_bc20_1.5kb L2 via Process #118
└─ C57_DBA_pool_25fmol L3 via Process #116
└─ C57_DBA_pool_run3 L3 via Process #122

3-D

Sample #399

Source Samples (Parents) 1

3-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 3-C57-CORTEX L1 via Process #115
└─ C57-3 L2 via Process #113

3-R

Sample #407

Source Samples (Parents) 1

3-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 1

C57_rep3_bc21
via Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)

All Ancestors 2

└─ 3-C57-CORTEX L1 via Process #115
└─ C57-3 L2 via Process #113

All Descendants 6

└─ C57_rep3_bc21 L1 via Process #97
└─ C57_DBA_pool_run1 L2 via Process #119
└─ C57_rep3_bc12 L2 via Process #121
└─ C57_rep3_bc21_1.5kb L2 via Process #118
└─ C57_DBA_pool_25fmol L3 via Process #116
└─ C57_DBA_pool_run3 L3 via Process #122

4-D

Sample #400

Source Samples (Parents) 1

4-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 4-C57-CORTEX L1 via Process #115
└─ C57-4 L2 via Process #113

4-R

Sample #408

Source Samples (Parents) 1

4-C57-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 4-C57-CORTEX L1 via Process #115
└─ C57-4 L2 via Process #113

7-D

Sample #401

Source Samples (Parents) 1

1-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 1-DBA-CORTEX L1 via Process #115
└─ DBA-1 L2 via Process #113

7-R

Sample #409

Source Samples (Parents) 1

1-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 1

DBA_rep1_bc22
via Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)

All Ancestors 2

└─ 1-DBA-CORTEX L1 via Process #115
└─ DBA-1 L2 via Process #113

All Descendants 6

└─ DBA_rep1_bc22 L1 via Process #97
└─ C57_DBA_pool_run1 L2 via Process #119
└─ DBA_rep1_bc13 L2 via Process #121
└─ DBA_rep1_bc22_1.5kb L2 via Process #118
└─ C57_DBA_pool_25fmol L3 via Process #116
└─ C57_DBA_pool_run3 L3 via Process #122

8-D

Sample #402

Source Samples (Parents) 1

2-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 2-DBA-CORTEX L1 via Process #115
└─ DBA-2 L2 via Process #113

8-R

Sample #410

Source Samples (Parents) 1

2-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 1

DBA_rep2_bc23
via Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)

All Ancestors 2

└─ 2-DBA-CORTEX L1 via Process #115
└─ DBA-2 L2 via Process #113

All Descendants 6

└─ DBA_rep2_bc23 L1 via Process #97
└─ C57_DBA_pool_run1 L2 via Process #119
└─ DBA_rep2_bc14 L2 via Process #121
└─ DBA_rep2_bc23_1.5kb L2 via Process #118
└─ C57_DBA_pool_25fmol L3 via Process #116
└─ C57_DBA_pool_run3 L3 via Process #122

9-D

Sample #403

Source Samples (Parents) 1

3-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 2

└─ 3-DBA-CORTEX L1 via Process #115
└─ DBA-3 L2 via Process #113

9-R

Sample #411

Source Samples (Parents) 1

3-DBA-CORTEX
via Process #115 — Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued

Derived Samples (Children) 1

DBA_rep3_bc24
via Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)

All Ancestors 2

└─ 3-DBA-CORTEX L1 via Process #115
└─ DBA-3 L2 via Process #113

All Descendants 6

└─ DBA_rep3_bc24 L1 via Process #97
└─ C57_DBA_pool_run1 L2 via Process #119
└─ DBA_rep3_bc15 L2 via Process #121
└─ DBA_rep3_bc24_1.5kb L2 via Process #118
└─ C57_DBA_pool_25fmol L3 via Process #116
└─ C57_DBA_pool_run3 L3 via Process #122

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