Equimolar pooling of C57/DBA barcoded PCR-cDNA libraries

Quantification and equimolar pooling of 6 barcoded PCR-cDNA libraries (3 C57BL/6, 3 DBA/2) at 2.27 fmol/µL target concentration, 25 fmol total in 11 µL. Libraries normalised with 4× scale-up dilution ...

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Process Details

Process ID: #116
Category: Sample_prep
Process Date: May 3, 2023
Created: February 23, 2026 am 07:26
Locked: Yes

Used in 1 research study:

Strain-specific cortex gene expression and isoform usage (C57BL/6 and DBA/2 Mouse Cortex Transcriptomics)

Linked Sequencing Runs 1

Platform	Run	Samples	Linked
Nanopore	Run #62	6	2026-02-21 17:54:37	View

Created Samples 1

C57_DBA_pool_25fmol
Equimolar pool of 6 barcoded PCR-cDNA libraries (C57 rep1-3 bc19-21, DBA rep1-3 bc22-24), 25 fmol in 11 µL, 2.27 fmol/µL, 1.5 kb average fragment

Source Samples 6

C57_rep1_bc19_1.5kb
Origin: Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
C57_rep2_bc20_1.5kb
Origin: Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
C57_rep3_bc21_1.5kb
Origin: Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
DBA_rep1_bc22_1.5kb
Origin: Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
DBA_rep2_bc23_1.5kb
Origin: Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
DBA_rep3_bc24_1.5kb
Origin: Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries

C57_DBA_pool_25fmol

Sample #445

Source Samples (Parents) 6

C57_rep1_bc19_1.5kb
via Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
C57_rep2_bc20_1.5kb
via Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
C57_rep3_bc21_1.5kb
via Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
DBA_rep1_bc22_1.5kb
via Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
DBA_rep2_bc23_1.5kb
via Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries
DBA_rep3_bc24_1.5kb
via Process #118 — AMPure XP 1.5 kb size selection of C57/DBA PCR-cDNA libraries

Derived Samples (Children) 0

No derived samples recorded.

All Ancestors 30

└─ C57_rep1_bc19_1.5kb L1 via Process #118
└─ C57_rep2_bc20_1.5kb L1 via Process #118
└─ C57_rep3_bc21_1.5kb L1 via Process #118
└─ DBA_rep1_bc22_1.5kb L1 via Process #118
└─ DBA_rep2_bc23_1.5kb L1 via Process #118
└─ DBA_rep3_bc24_1.5kb L1 via Process #118
└─ C57_rep1_bc19 L2 via Process #97
└─ C57_rep2_bc20 L2 via Process #97
└─ C57_rep3_bc21 L2 via Process #97
└─ DBA_rep1_bc22 L2 via Process #97
└─ DBA_rep2_bc23 L2 via Process #97
└─ DBA_rep3_bc24 L2 via Process #97
└─ 1-R L3 via Process #111
└─ 2-R L3 via Process #111
└─ 3-R L3 via Process #111
└─ 7-R L3 via Process #111
└─ 8-R L3 via Process #111
└─ 9-R L3 via Process #111
└─ 1-C57-CORTEX L4 via Process #115
└─ 1-DBA-CORTEX L4 via Process #115
└─ 2-C57-CORTEX L4 via Process #115
└─ 2-DBA-CORTEX L4 via Process #115
└─ 3-C57-CORTEX L4 via Process #115
└─ 3-DBA-CORTEX L4 via Process #115
└─ C57-1 L5 via Process #113
└─ C57-2 L5 via Process #113
└─ C57-3 L5 via Process #113
└─ DBA-1 L5 via Process #113
└─ DBA-2 L5 via Process #113
└─ DBA-3 L5 via Process #113

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