Libre Biotech

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15)

Re-amplification of existing cDNA from original lib prep with new barcodes (bc10-15) for a third sequencing run. PCR from old cDNAs, not a fresh reverse transcription.

Status
Completed
Files
0
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Team
0
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Used in Studies
1
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Process Details
Process ID
#121
Category
Sample_prep
Process Date
May 9, 2023
Created
February 23, 2026 am 08:55
Locked
Yes
Used in 1 research study:
Linked Sequencing Runs 2
Platform Run Samples Linked
Nanopore Run #61 6 2026-02-21 17:40:01 View
Nanopore Run #63 6 2026-02-21 17:54:37 View
Created Samples 6
  • C57_rep1_bc10
    C57 rep1 re-barcoded library (bc10) from existing cDNA
  • C57_rep2_bc11
    C57 rep2 re-barcoded library (bc11) from existing cDNA
  • C57_rep3_bc12
    C57 rep3 re-barcoded library (bc12) from existing cDNA
  • DBA_rep1_bc13
    DBA rep1 re-barcoded library (bc13) from existing cDNA
  • DBA_rep2_bc14
    DBA rep2 re-barcoded library (bc14) from existing cDNA
  • DBA_rep3_bc15
    DBA rep3 re-barcoded library (bc15) from existing cDNA
Source Samples 6
  • C57_rep1_bc19
    Origin: Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)
  • C57_rep2_bc20
    Origin: Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)
  • C57_rep3_bc21
    Origin: Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)
  • DBA_rep1_bc22
    Origin: Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)
  • DBA_rep2_bc23
    Origin: Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)
  • DBA_rep3_bc24
    Origin: Process #97 — PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24)
C57_rep1_bc10
Sample #453
Source Samples (Parents) 1
Derived Samples (Children) 1
All Ancestors 4
All Descendants 1
C57_rep2_bc11
Sample #454
Source Samples (Parents) 1
Derived Samples (Children) 1
All Ancestors 4
All Descendants 1
C57_rep3_bc12
Sample #455
Source Samples (Parents) 1
Derived Samples (Children) 1
All Ancestors 4
All Descendants 1
DBA_rep1_bc13
Sample #456
Source Samples (Parents) 1
Derived Samples (Children) 1
All Ancestors 4
All Descendants 1
DBA_rep2_bc14
Sample #457
Source Samples (Parents) 1
Derived Samples (Children) 1
All Ancestors 4
All Descendants 1
DBA_rep3_bc15
Sample #458
Source Samples (Parents) 1
Derived Samples (Children) 1
All Ancestors 4
All Descendants 1
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Not Reviewed
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