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Data Transformation: Mouse Long-Read RNA-Seq (ONT PCR-cDNA) (Run #2)

Auto-created from analysis run #2 (mouse-rnaseq pipeline)

Status
Completed
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Files
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Used in Studies
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Assay Workflow: Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA 1.5kb (run 2) (sequencing assay → this data transformation)
Process Details
Process ID
#128
Category
Data_transformation
Process Date
February 23, 2026
Created
March 17, 2026 am 03:33
Locked
Yes
Used in 1 research study:
Workflow Inputs
Input File Description
FASTQ reads fastq_pass/{barcode}/*.fastq.gz Nanopore basecalled reads per barcode
Reference genome GRCm39.primary_assembly.genome.fa Mouse genome assembly
Gene annotation gencode.vM35.annotation.gtf GENCODE mouse annotation
Output Files View Full Results
File Format Size
C57_rep1.sorted.bam application/octet-stream 715.2 MB
C57_rep1.sorted.bam.bai application/octet-stream 1.6 MB
C57_rep2.sorted.bam application/octet-stream 721.8 MB
C57_rep2.sorted.bam.bai application/octet-stream 1.6 MB
C57_rep3.sorted.bam application/octet-stream 189.2 MB
C57_rep3.sorted.bam.bai application/octet-stream 1.4 MB
DBA_rep1.sorted.bam application/octet-stream 975 MB
DBA_rep1.sorted.bam.bai application/octet-stream 1.6 MB
DBA_rep2.sorted.bam application/octet-stream 760.3 MB
DBA_rep2.sorted.bam.bai application/octet-stream 1.6 MB
DBA_rep3.sorted.bam application/octet-stream 881.4 MB
DBA_rep3.sorted.bam.bai application/octet-stream 1.6 MB
bambu_summary.txt text/plain 190 B
counts_gene.txt text/plain 5 MB
counts_transcript.txt text/plain 5 MB
extended_annotations.gtf text/plain 124 MB
dge_results.csv text/csv 1.9 MB
dge_summary.txt text/plain 1.2 KB
ma_plot.pdf application/pdf 857.5 KB
normalized_counts.csv text/csv 1.7 MB
pca_plot.pdf application/pdf 5.1 KB
transcript_gene_names.tsv text/tab-separated-values 4 MB
volcano_plot.pdf application/pdf 832.6 KB
execution_report.html text/html 2.8 MB
pipeline_timeline.html text/html 249.7 KB
samplesheet.csv text/csv 480 B
Pipeline / Analysis Details
Analysis Run
Run
#2 — Mouse Long-Read RNA-Seq (ONT PCR-cDNA)
Status
succeeded
Completed
2026-02-23 21:11:26
Pipeline
mouse-rnaseq
Description
Auto-created from analysis run #2 (mouse-rnaseq pipeline)
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