Data Transformation: Mouse Long-Read RNA-Seq (ONT PCR-cDNA) (Run #6)
Auto-created from analysis run #6 (mouse-rnaseq pipeline)
Status
Completed
✓
Files
0
📎
Team
0
👥
Used in Studies
1
🔬
Assay Workflow:
Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3)
(sequencing assay → this data transformation)
Process Details
- Process ID
#132- Category
- Data_transformation
- Process Date
- February 24, 2026
- Created
- March 17, 2026 am 03:36
- Locked
- Yes
Used in 1 research study:
- Strain-specific cortex gene expression and isoform usage (C57BL/6 and DBA/2 Mouse Cortex Transcriptomics)
Workflow Inputs
| Input | File | Description |
|---|---|---|
| FASTQ reads | fastq_pass/{barcode}/*.fastq.gz |
Nanopore basecalled reads per barcode |
| Reference genome | GRCm39.primary_assembly.genome.fa |
Mouse genome assembly |
| Gene annotation | gencode.vM35.annotation.gtf |
GENCODE mouse annotation |
Output Files
View Full Results
| File | Format | Size |
|---|---|---|
main.nf |
application/octet-stream | 4.6 KB |
samplesheet.csv |
text/csv | 456 B |
execution_report.html |
text/html | 2.8 MB |
pipeline_timeline.html |
text/html | 249.5 KB |
dge_results.csv |
text/csv | 1.3 MB |
volcano_plot.pdf |
application/pdf | 526.1 KB |
transcript_gene_names.tsv |
text/tab-separated-values | 260.6 KB |
ma_plot.pdf |
application/pdf | 539.7 KB |
normalized_counts.csv |
text/csv | 1.1 MB |
pca_plot.pdf |
application/pdf | 5.1 KB |
dge_summary.txt |
text/plain | 1.3 KB |
C57_rep3.sorted.bam |
application/octet-stream | 243.6 MB |
DBA_rep2.sorted.bam.bai |
application/octet-stream | 1.5 MB |
DBA_rep1.sorted.bam |
application/octet-stream | 248.7 MB |
DBA_rep1.sorted.bam.bai |
application/octet-stream | 1.5 MB |
C57_rep3.sorted.bam.bai |
application/octet-stream | 1.4 MB |
DBA_rep2.sorted.bam |
application/octet-stream | 283.5 MB |
C57_rep1.sorted.bam |
application/octet-stream | 207.2 MB |
C57_rep2.sorted.bam |
application/octet-stream | 222.6 MB |
C57_rep1.sorted.bam.bai |
application/octet-stream | 1.4 MB |
C57_rep2.sorted.bam.bai |
application/octet-stream | 1.4 MB |
DBA_rep3.sorted.bam |
application/octet-stream | 266.5 MB |
DBA_rep3.sorted.bam.bai |
application/octet-stream | 1.5 MB |
counts_gene.txt |
text/plain | 4.9 MB |
counts_transcript.txt |
text/plain | 4.9 MB |
extended_annotations.gtf |
text/plain | 123.6 MB |
bambu_summary.txt |
text/plain | 189 B |
Pipeline / Analysis Details
Analysis Run
- Run
- #6 — Mouse Long-Read RNA-Seq (ONT PCR-cDNA)
- Status
- succeeded
- Completed
- 2026-02-24 09:10:40
- Pipeline
mouse-rnaseq
Description
Auto-created from analysis run #6 (mouse-rnaseq pipeline)
Attached Files
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Approval Status
Not Reviewed
Activity History
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