Ligation sequencing DNA (SQK-LSK109) library preparation
Adapter ligation library prep for nanopore sequencing using the legacy SQK-LSK109 kit.
Version History
Procedure Details
Equipment (Catalog)
8
-
Microcentrifuge
Centrifugation
Microcentrifuge for brief spins -
Micropipette
Liquid handling
Micropipettes -
Magnetic rack
Magnetic separation
Magnetic rack for bead cleanup -
Fluorometer
Measurement
Qubit fluorometer for library QC -
Rotator mixer
Mixing
Rotator mixer for bead binding -
Vortex mixer
Mixing
Vortex mixer -
Nanopore sequencer
Sequencing
MinION / GridION sequencer -
Thermal cycler
Thermal regulation
Thermal cycler for end-prep and ligation
Materials (Catalog)
7
-
Ethanol
Chemical
80% freshly prepared ethanol for bead washes -
Microcentrifuge tube
Consumable
1.5 mL Eppendorf DNA LoBind tubes -
Pipette tips
Consumable
Wide-bore pipette tips for DNA handling -
NEBNext Companion Module for ONT Ligation Sequencing
(NEB)
E7180SLibrary preparation kit
NEBNext Companion Module for ONT (E7180S) -
Ligation Sequencing Kit
(Oxford Nanopore)
SQK-LSK109Sequencing kit
Ligation Sequencing Kit (SQK-LSK109) -
AMPure XP
(Beckman Coulter)
A63881Magnetic beads (SPRI)
AMPure XP beads (A63881) -
Nuclease-free water
Reagent
For elution
Procedure Steps (Version 1.0)
No steps defined for this version.
References
- Oxford Nanopore Technologies — gDNA (SQK-LSK109) Protocol, Rev B, 24 Feb 2023 Link protocol