Libre Biotech

PacBio CCS (Subreads → HiFi)

CCS: C57 I-K1 (retry with pbindex)

Type
Custom
Status
succeeded
Engine
ccs 6.4.0
Duration
0.8 h

Software Tools

Results Summary

CCS Reads
133,585
Mean Length
2,563 bp
Mean Passes
0.0
Conversion
31.8%

Output Files

ccs.bam HPC 313.5 MB ccs.bam.pbi HPC 1.2 MB ccs.ccs_report.txt HPC 888 B ccs.report.json HPC 3.4 KB ccs.zmw_metrics.json.gz HPC 15.5 MB results_summary.json HPC 164 B

Provenance

Execution PacBio CCS: subreads to HiFi (mouse_c57_IK1)
Started 2026-03-04T16:36:10+00:00
Completed 2026-03-04T07:22:37+00:00
RO-Crate 1.1 Workflow RO-Crate 1.0 FAIR
This analysis is packaged as a Research Object Crate with machine-readable provenance and FAIR metadata.

RO-Crate Metadata (JSON-LD)

Show/hide raw JSON-LD
{
    "@context": "https://w3id.org/ro/crate/1.1/context",
    "@graph": [
        {
            "@id": "ro-crate-metadata.json",
            "@type": "CreativeWork",
            "about": {
                "@id": "./"
            },
            "conformsTo": [
                {
                    "@id": "https://w3id.org/ro/crate/1.1"
                },
                {
                    "@id": "https://w3id.org/workflowhub/workflow-ro-crate/1.0"
                }
            ]
        },
        {
            "@id": "./",
            "@type": "Dataset",
            "name": "PacBio CCS (mouse_c57_IK1) \u2014 Run #74",
            "description": "PacBio CCS: subreads to HiFi consensus reads",
            "datePublished": "2026-03-04",
            "license": {
                "@id": "https://creativecommons.org/licenses/by/4.0/"
            },
            "hasPart": [
                {
                    "@id": "ccs.bam.pbi"
                },
                {
                    "@id": "ccs.zmw_metrics.json.gz"
                },
                {
                    "@id": "ccs.bam"
                },
                {
                    "@id": "ccs.ccs_report.txt"
                },
                {
                    "@id": "ccs.report.json"
                },
                {
                    "@id": "results_summary.json"
                }
            ],
            "mentions": [
                {
                    "@id": "#execution"
                }
            ]
        },
        {
            "@id": "#ccs",
            "@type": "SoftwareApplication",
            "name": "ccs",
            "softwareVersion": "6.4.0",
            "url": {
                "@id": "https://github.com/PacificBiosciences/ccs"
            }
        },
        {
            "@id": "#pbindex",
            "@type": "SoftwareApplication",
            "name": "pbindex",
            "url": {
                "@id": "https://github.com/PacificBiosciences/pbbam"
            }
        },
        {
            "@id": "ccs.bam.pbi",
            "@type": "File",
            "name": "ccs.bam.pbi",
            "encodingFormat": "application/octet-stream",
            "contentSize": "1.2 MB",
            "sha256": "c4bac6b754b7f242ed40f3d88d2a6c9be54fefa1c571692ca71e654ef8fd2e25"
        },
        {
            "@id": "ccs.zmw_metrics.json.gz",
            "@type": "File",
            "name": "ccs.zmw_metrics.json.gz",
            "encodingFormat": "application/octet-stream",
            "contentSize": "15.5 MB",
            "sha256": "a977beeb8ae1c79529df098d24d918d8652244c2f21d17acd57c85ab39b9cad2"
        },
        {
            "@id": "ccs.bam",
            "@type": "File",
            "name": "ccs.bam",
            "encodingFormat": "application/octet-stream",
            "contentSize": "313.5 MB",
            "sha256": "ef30700d8c4c0c85858416d3561294107c717a6134b8b436f07c8c7d6a70b19b"
        },
        {
            "@id": "ccs.ccs_report.txt",
            "@type": "File",
            "name": "ccs.ccs_report.txt",
            "encodingFormat": "application/octet-stream",
            "contentSize": "888 B",
            "sha256": "1810566e97afc4e06a8a34cde77f9ccdf23bce9fd9d0e3a78d36ca755ec91c82"
        },
        {
            "@id": "ccs.report.json",
            "@type": "File",
            "name": "ccs.report.json",
            "encodingFormat": "application/json",
            "contentSize": "3.4 KB",
            "sha256": "f9425bf89e3e27b62797df51d3cd8bc26bf1d2ddaa0636d72e24c80c0f05c199"
        },
        {
            "@id": "#execution",
            "@type": "CreateAction",
            "name": "PacBio CCS: subreads to HiFi (mouse_c57_IK1)",
            "instrument": {
                "@id": "#ccs"
            },
            "startTime": "2026-03-04T16:36:10+00:00",
            "endTime": "2026-03-04T07:22:37+00:00",
            "result": [
                {
                    "@id": "ccs.bam.pbi"
                },
                {
                    "@id": "ccs.zmw_metrics.json.gz"
                },
                {
                    "@id": "ccs.bam"
                },
                {
                    "@id": "ccs.ccs_report.txt"
                },
                {
                    "@id": "ccs.report.json"
                }
            ]
        },
        {
            "@id": "results_summary.json",
            "@type": "File",
            "name": "results_summary.json",
            "description": "CCS conversion summary statistics",
            "encodingFormat": "application/json",
            "contentSize": "164 B",
            "sha256": "5c76f30942458a674de702bfccfcc8842279c3c1158dfbc9728827f7bfc99d71"
        }
    ]
}