ONT Isoform Functional Annotation (superseded)

Barcode 19 functional annotation

Download ZIP JSON-LD

Type

CWL

Status

succeeded

Engine

cwltool

Duration

2 h

Source Data

Study	Strain-specific cortex gene expression and isoform usage
Sample prep	PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) 2023-05-09
Sequencing	Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) 2023-05-12
Run Data	Run #63 (6 samples)
Samples	C57_rep1_bc10 C57_rep2_bc11 C57_rep3_bc12 DBA_rep1_bc13 DBA_rep2_bc14 DBA_rep3_bc15

Pipeline

ONT Long-Read Transcriptomics (IsoQuant)

Run #47

succeeded

ONT Isoform Functional Annotation (superseded)

Run #48 (this run)

succeeded 1 sources

Combined From

#47 — ONT Long-Read Transcriptomics (IsoQuant) succeeded

Sample Provenance

Process Chain

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

8 samples produced

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

24 samples produced

Simultaneous extraction of DNA and RNA from C57 and DBA cortex tissue using Qiagen AllPrep (2016-11-17) Labbook

Protocol: Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit

16 samples produced

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24) (2023-03-13) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection) (2023-03-14) Labbook

24 measurements across 6 samples

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) (2023-05-09) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) (2023-05-12) Labbook

QC Measurements

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection)

2023-03-14 Labbook

Sample	bp	count	ng/µL	nmol/L
C57_rep1_bc19	1493	3	5.6008	6.1563
C57_rep2_bc20	1493	4	6.2061	7.4416
C57_rep3_bc21	1485	3	5.7241	6.8672
DBA_rep1_bc22	1500	2	4.2355	4.4695
DBA_rep2_bc23	1485	2	4.0452	4.2648
DBA_rep3_bc24	1485	2	4.3327	4.7107

Workflow

ONT Isoform Functional Annotation

#cwl

Software Tools

Tool	Version	URL
cwltool	-	https://github.com/common-workflow-language/cwltool
eldudy007_isoseq:1.0.sif	-	-

Results Summary

Unique Reads

399,605

from upstream transcriptomics

rRNA Reads

296 (0.1%)

mRNA Reads

399,309

used for CPM calculation

Transcripts Annotated

10,114

Pfam Hit Rate

93.9%

SwissProt Hit Rate

95.0%

High-Confidence

7,528 (74.4%)

Tier 1–2

NMD Candidates

252 (2.5%)

Evidence Tier Distribution

Novel Isoform Discovery

Total Isoforms

10,114

Reference

9,381

Novel (IsoQuant)

733

728 with ≥3 reads, 5 low-support

Novel %

7.2%

DV Genes: Novel-Created

199

Variation only from novel isoforms

DV Genes: Novel-Contributed

Novel isoforms add to existing ref variation

DV Genes: Reference-Only

540

Variation from reference annotation alone

Long-read sequencing discovered 733 novel isoforms (7.2%). Of 789 domain variant genes, 199 (25%) show domain architecture variation only because of novel isoforms not present in the reference annotation. In 5 DV genes, only novel isoforms produced detectable ORFs.

Isoform Read Support (CPM from upstream transcriptomics)

Novel Expression Rate

100.0%

733 / 733 (728 with ≥3 reads)

Reference Expression Rate

94.9%

8,904 / 9,381

Novel Median CPM

15.0

median 6 reads (mean 11.3, range 0–279)

Ref Median CPM

12.5

Novel (n=733)

Reference (n=9,381)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual isoform CPM values are overlaid as jittered points. Log₁₀ scale; dashed line marks CPM = 1 expression threshold. Per-model CPM from OUT.transcript_model_counts.tsv

Novel Isoform Functional Impact

Domain Altered

11.1% of novel

NMD Candidates

6.3% of novel

ORF Disrupted

11.5% of novel

Conserved

494

67.4% — same protein

690 of 733 novel isoforms (94.1%) have SwissProt protein homologs. 28 isoforms have no detectable protein homolog. Top species: Mus musculus (599), Bos taurus (31), Homo sapiens (26), Rattus norvegicus (22), Macaca fascicularis (4).

High-Impact Novel Isoforms (150)

Gene	Impact	Details	ORF	Reads	CPM	SwissProt
EEF1A1 `transcript1361.9.nnic`	Domain altered	Lost: ALR_C-like	complete	54	135.2	sp\|P68103\|EF1A1_BOVIN 50.6% — Elongation factor 1-alpha 1
TUBB2A `transcript213.13.nnic`	Domain altered	Lost: Tubulin_C	complete	41	102.7	sp\|P09203\|TBB1_CHICK 99.4% — Tubulin beta-1 chain
Igfbp4 `transcript1941.11.nnic`	Domain altered	Lost: GF_recep_C-rich	complete	30	75.1	sp\|P47879\|IBP4_MOUSE 100.0% — Insulin-like growth factor-bin...
Il18 `transcript690.9.nic`	Domain altered	Gained: GT-A	complete	29	72.6	sp\|P70380\|IL18_MOUSE 100.0% — Interleukin-18
Bin1 `transcript183.18.nnic`	Domain altered	Lost: CK2S, DUF1664, Jnk-SapK_ap_N, OmpH, PTS_IIA, SNARE-fusion, TBD	complete	28	70.1	sp\|O08539\|BIN1_MOUSE 93.9% — Myc box-dependent-interacting ...
RBBP4 `transcript1144.4.nic`	Domain altered	Lost: Hexon	complete	24	60.1	sp\|Q3MHL3\|RBBP4_BOVIN 95.5% — Histone-binding protein RBBP4
Thoc7 `transcript45.14.nnic`	Domain altered	Gained: Pec_lyase-like, Tup_N, V_ATPase_I	complete	18	45.1	sp\|Q7TMY4\|THOC7_MOUSE 100.0% — THO complex subunit 7 homolog
Laptm4a `transcript55.12.nnic`	Domain altered	Lost: Circovir2_Orf4	complete	18	45.1	sp\|Q60961\|LAP4A_MOUSE 100.0% — Lysosomal-associated transmemb...
Cpe `transcript467.8.nnic`	Domain altered	Lost: Transthyretin	complete	15	37.6	sp\|Q00493\|CBPE_MOUSE 100.0% — Carboxypeptidase E
Zwint `transcript504.10.nnic`	Domain altered	Lost: FHIPEP	complete	14	35.1	sp\|Q9CQU5\|ZWINT_MOUSE 100.0% — Outer kinetochore KNL1 complex...
Psmb10 `transcript1433.8.nic`	Domain altered	Gained: 6PGD_C	complete	13	32.6	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Tpd52l2 `transcript2833.2.nic`	Domain altered	Lost: DUF4164, Glyco_hydro_36, P-loop_NTPase, VBS-like	complete	13	32.6	sp\|Q9CYZ2\|TPD54_MOUSE 87.3% — Tumor protein D54
TSC22D1 `transcript1065.14.nnic`	Domain altered +NMD	Lost: DUF2946	complete	12	30.1	sp\|Q3MHL6\|T22D1_BOVIN 98.6% — TSC22 domain family protein 1
Rbck1 `transcript2094.2.nnic`	Domain altered	Lost: Rad50_zn_hook, Ubiquitin, zf-RanBP	complete	12	30.1	sp\|Q9WUB0\|HOIL1_MOUSE 97.7% — RanBP-type and C3HC4-type zinc...
CPLX2 `transcript449.13.nnic`	Domain altered	Lost: DUF2838	complete	12	30.1	sp\|P84088\|CPLX2_BOVIN 100.0% — Complexin-2
Pbx4 `transcript520.8.nic`	Domain altered	Lost: Carbpep_Y_N, DUF1479, HTH, P-loop_NTPase, YdaS_antitoxin	complete	12	30.1	sp\|Q99NE9\|PBX4_MOUSE 36.5% — Pre-B-cell leukemia transcript...
Nap1l1 `transcript1158.10.nnic`	Domain altered	Gained: SH3, TPR	complete	11	27.6	sp\|P28656\|NP1L1_MOUSE 99.4% — Nucleosome assembly protein 1-...
Eef1e1 `transcript315.13.nnic`	Domain altered	Lost: GST_C	complete	11	27.6	sp\|Q9D1M4\|MCA3_MOUSE 81.6% — Eukaryotic translation elongat...
Cuta `transcript552.17.nic`	Domain altered +NMD	Lost: TIM_barrel	complete	10	25.0	sp\|Q9CQ89\|CUTA_MOUSE 99.0% — Protein CutA
Cops4 `transcript820.5.nic`	Domain altered	Lost: YabA	complete	10	25.0	sp\|O88544\|CSN4_MOUSE 100.0% — COP9 signalosome complex subun...
Polb `transcript156.8.nnic`	Domain altered	Lost: HHH, WSN	complete	9	22.5	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Gnptg `transcript382.17.nic`	Domain altered	Lost: AltA1	complete	9	22.5	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Bax `transcript1360.7.nnic`	Domain altered	Lost: CF222	complete	8	20.0	sp\|Q07813\|BAX_MOUSE 100.0% — Apoptosis regulator BAX
Cdc123 `transcript27.2.nnic`	Domain altered	Lost: Beta_propeller	complete	8	20.0	sp\|Q8CII2\|CD123_MOUSE 87.5% — Translation initiation factor ...
Rps2 `transcript318.17.nic`	Domain altered +NMD	Lost: S5	complete	8	20.0	sp\|P25444\|RS2_MOUSE 100.0% — Small ribosomal subunit protei...
TSC22D1 `transcript1060.14.nnic`	Domain altered +NMD	Lost: DUF2946	complete	7	17.5	sp\|Q3MHL6\|T22D1_BOVIN 98.6% — TSC22 domain family protein 1
TSC22D1 `transcript1079.14.nnic`	Domain altered +NMD	Lost: DUF2946	complete	7	17.5	sp\|Q3MHL6\|T22D1_BOVIN 98.6% — TSC22 domain family protein 1
Nap1l1 `transcript1162.10.nnic`	Domain altered	Gained: Nucleoplasmin	complete	7	17.5	sp\|P28656\|NP1L1_MOUSE 99.0% — Nucleosome assembly protein 1-...
Zdhhc4 `transcript1960.5.nic`	Domain altered	Gained: DoxD-like	complete	7	17.5	sp\|Q9D6H5\|ZDHC4_MOUSE 100.0% — Palmitoyltransferase ZDHHC4
Nsmce1 `transcript2310.7.nnic`	Domain altered	Gained: TMEMspv1-c74-12	complete	7	17.5	sp\|Q9D720\|NSE1_MOUSE 95.1% — Non-structural maintenance of ...
Tmem191 `transcript257.16.nic`	Domain altered	Gained: DUF745, NPV_P10, pRN1_helical	complete	7	17.5	sp\|Q9JJB1\|T191_MOUSE 100.0% — Transmembrane protein 191
Prxl2a `transcript383.14.nic`	Domain altered	Lost: 2heme_cytochrom	complete	7	17.5	sp\|Q9CYH2\|PXL2A_MOUSE 100.0% — Peroxiredoxin-like 2A
Pacrg `transcript65.17.nic`	Domain altered	Gained: Peptidase_PA, RNA_pol_Rpb2_2	complete	7	17.5	sp\|Q9DAK2\|PACRG_MOUSE 100.0% — Parkin coregulated gene protei...
Coq2 `transcript824.5.nic`	Domain altered	Lost: DUF2798	complete	7	17.5	sp\|Q66JT7\|COQ2_MOUSE 100.0% — 4-hydroxybenzoate polyprenyltr...
Mog `transcript1108.17.nnic`	Domain altered	Lost: PF	complete	6	15.0	sp\|Q61885\|MOG_MOUSE 97.1% — Myelin-oligodendrocyte glycopr...
Slc13a5 `transcript1265.11.nic`	Domain altered	Gained: DAGK_prokar	complete	6	15.0	sp\|Q67BT3\|S13A5_MOUSE 99.5% — Na(+)/citrate cotransporter
Rpl14 `transcript1329.7.nnic`	Domain altered	Lost: UPF0688	complete	6	15.0	sp\|Q9CR57\|RL14_MOUSE 100.0% — Large ribosomal subunit protei...
Ckmt1 `transcript1483.2.nnic`	Domain altered	Lost: ATP-gua_PtransN	complete	6	15.0	sp\|P30275\|KCRU_MOUSE 100.0% — Creatine kinase U-type, mitoch...
Poc1a `transcript1606.9.nnic`	Domain altered	Lost: NTN, Nup160	complete	6	15.0	sp\|Q8JZX3\|POC1A_MOUSE 100.0% — POC1 centriolar protein homolo...
Rpn2 `transcript2321.2.nic`	Domain altered	Lost: DUF5859, TPR, Ubiquitin	complete	6	15.0	sp\|Q9DBG6\|RPN2_MOUSE 100.0% — Dolichyl-diphosphooligosacchar...
Araf `transcript275.X.nic`	Domain altered	Lost: PKinase	complete	6	15.0	sp\|P04627\|ARAF_MOUSE 100.0% — Serine/threonine-protein kinas...
Dctn1 `transcript687.6.nnic`	Domain altered	Lost: DUF2935, DUF4172, Dynactin, Fe_hyd_lg_C, Fez1, Filament	complete	6	15.0	sp\|O08788\|DCTN1_MOUSE 100.0% — Dynactin subunit 1
Gap43 `transcript739.16.nnic`	Domain altered	Lost: EF_hand, GMC, Neuromodulin_N	complete	6	15.0	—
Mrps15 `transcript1022.4.nic`	Domain altered	Lost: GPS2_interact	complete	5	12.5	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Ubac2 `transcript1202.14.nnic`	Domain altered	Gained: SH3, TMEM239	complete	5	12.5	sp\|Q8R1K1\|UBAC2_MOUSE 100.0% — Ubiquitin-associated domain-co...
Psmb10 `transcript1434.8.nic`	Domain altered	Gained: 6PGD_C	complete	5	12.5	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Ubc `transcript1436.5.nnic`	Domain altered	Gained: DUF6393	complete	5	12.5	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Morf4l1 `transcript1486.9.nnic`	Domain altered	Lost: MRG	complete	5	12.5	sp\|P60762\|MO4L1_MOUSE 96.2% — Mortality factor 4-like protei...
ACTL6B `transcript1717.5.nic`	Domain altered	Lost: Rep	complete	5	12.5	sp\|A4FUX8\|ACL6B_BOVIN 100.0% — Actin-like protein 6B
Sap30bp `transcript2494.11.nnic`	Domain altered	Gained: CCDC84	complete	5	12.5	sp\|Q02614\|S30BP_MOUSE 94.8% — SAP30-binding protein
Araf `transcript267.X.nnic`	Domain altered	Lost: PKinase	complete	5	12.5	sp\|P04627\|ARAF_MOUSE 100.0% — Serine/threonine-protein kinas...
Cltb `transcript515.13.nnic`	Domain altered	Lost: CENP-Q, DUF6161, GED	complete	5	12.5	sp\|Q6IRU5\|CLCB_MOUSE 98.2% — Clathrin light chain B
Chmp3 `transcript533.6.nic`	Domain altered	Lost: SPOUT	complete	5	12.5	sp\|Q9CQ10\|CHMP3_MOUSE 100.0% — Charged multivesicular body pr...
Ubc `transcript1413.5.nnic`	Domain altered	Gained: DUF6393	complete	4	10.0	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Ndufs2 `transcript1423.1.nnic`	Domain altered	Lost: NiFeSe_Hases	complete	4	10.0	sp\|Q91WD5\|NDUS2_MOUSE 70.0% — NADH dehydrogenase [ubiquinone...
Htatip2 `transcript1495.7.nic`	Domain altered	Lost: Periplas_BP	complete	4	10.0	sp\|Q9Z2G9\|HTAI2_MOUSE 100.0% — Protein HTATIP2
Blvra `transcript1577.2.nnic`	Domain altered	Lost: NADP_Rossmann, SAM	complete	4	10.0	sp\|Q9CY64\|BIEA_MOUSE 100.0% — Biliverdin reductase A
Poc1a `transcript1605.9.nnic`	Domain altered	Lost: NTN, Nup160	complete	4	10.0	sp\|Q8JZX3\|POC1A_MOUSE 100.0% — POC1 centriolar protein homolo...
Myl4 `transcript2259.11.nnic`	Domain altered	Gained: HTH	complete	4	10.0	sp\|P09541\|MYL4_MOUSE 100.0% — Myosin light chain 4
Kat5 `transcript297.19.nic`	Domain altered	Lost: SH3	complete	4	10.0	sp\|Q5RBG4\|KAT5_PONAB 100.0% — Histone acetyltransferase KAT5
Slc35a4 `transcript320.18.nic`	Domain altered	Lost: DUF4535	complete	4	10.0	sp\|Q9D321\|S35A4_MOUSE 100.0% — Probable UDP-sugar transporter...
Nme3 `transcript353.17.nnic`	Domain altered	Lost: Peptidase_SH, TIM_barrel	complete	4	10.0	sp\|Q9WV85\|NDK3_MOUSE 100.0% — Nucleoside diphosphate kinase ...
Khdrbs3 `transcript357.15.nnic`	Domain altered	Lost: FSA_C, STAR_dimer	complete	4	10.0	sp\|Q9R226\|KHDR3_MOUSE 100.0% — KH domain-containing, RNA-bind...
Gnptg `transcript385.17.nnic`	Domain altered	Lost: AltA1	complete	4	10.0	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Arpc2 `transcript503.1.nic`	Domain altered	Gained: Nucleoplasmin	complete	4	10.0	sp\|Q9CVB6\|ARPC2_MOUSE 99.6% — Actin-related protein 2/3 comp...
Rnf181 `transcript571.6.nnic`	Domain altered	Lost: BIR-like, C2H2-zf, Rad50_zn_hook	complete	4	10.0	sp\|Q9CY62\|RN181_MOUSE 84.8% — E3 ubiquitin-protein ligase RN...
Kxd1 `transcript621.8.nnic`	Domain altered	Lost: Sde2_N_Ubi, TolA_bind_tri, Ubiquitin, Zn_Beta_Ribbon	complete	4	10.0	sp\|Q80XH1\|KXDL1_MOUSE 71.2% — KxDL motif-containing protein ...
Tmed9 `transcript630.13.nnic`	Domain altered	Gained: UPF0128	complete	4	10.0	sp\|Q3T133\|TMED9_BOVIN 100.0% — Transmembrane emp24 domain-con...
Cct5 `transcript86.15.nic`	Domain altered	Lost: E-set	complete	4	10.0	sp\|P80316\|TCPE_MOUSE 100.0% — T-complex protein 1 subunit ep...
Mrps18b `transcript1046.17.nnic`	Domain altered	Lost: RING	complete	3	7.5	sp\|Q99N84\|RT18B_MOUSE 99.3% — Small ribosomal subunit protei...
Polb `transcript149.8.nnic`	Domain altered	Lost: HHH, WSN	complete	3	7.5	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Polb `transcript155.8.nnic`	Domain altered	Lost: HHH, WSN	complete	3	7.5	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Bfar `transcript161.16.nic`	Domain altered	Gained: DUF2076	complete	3	7.5	sp\|Q8R079\|BFAR_MOUSE 99.7% — Bifunctional apoptosis regulat...
Snf8 `transcript1803.11.nic`	Domain altered	Lost: Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C, ZapD	complete	3	7.5	sp\|Q9CZ28\|SNF8_MOUSE 100.0% — Vacuolar-sorting protein SNF8
Ascc1 `transcript329.10.nnic`	Domain altered	Lost: KH	complete	3	7.5	sp\|Q9D8Z1\|ASCC1_MOUSE 99.6% — Activating signal cointegrator...
Gnptg `transcript392.17.nic`	Domain altered	Lost: AltA1	complete	3	7.5	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Brinp1 `transcript549.4.nnic`	Domain altered	Lost: CDC	complete	3	7.5	sp\|Q920P3\|BRNP1_MOUSE 100.0% — BMP/retinoic acid-inducible ne...
Farsb `transcript657.1.nic`	Domain altered	Lost: Death	complete	3	7.5	sp\|Q9WUA2\|SYFB_MOUSE 100.0% — Phenylalanine--tRNA ligase bet...
Nol12 `transcript706.15.nic`	Domain altered	Lost: ALC	complete	3	7.5	sp\|Q8BG17\|NOL12_MOUSE 89.9% — Nucleolar protein 12
Cers2 `transcript803.3.nic`	Domain altered	Lost: HTH, TRAM1, UPF0767	complete	3	7.5	sp\|Q924Z4\|CERS2_MOUSE 100.0% — Ceramide synthase 2
Exosc1 `transcript943.19.nic`	Domain altered	Lost: Hybrid	complete	3	7.5	sp\|Q9DAA6\|EXOS1_MOUSE 100.0% — Exosome complex component CSL4
Mbp `transcript894.18.nnic`	ORF disrupted		5prime_partial	178	445.8	—
Rtn1 `transcript445.12.nnic`	ORF disrupted	Lost: DUF4736	5prime_partial	146	365.6	sp\|Q64548\|RTN1_RAT 100.0% — Reticulon-1
Grb14 `transcript838.2.nic`	ORF disrupted	Lost: BPS, PH, Ubiquitin	5prime_partial	60	150.3	sp\|Q9JLM9\|GRB14_MOUSE 88.3% — Growth factor receptor-bound p...
Ndrg2 `transcript500.14.nic`	ORF disrupted		5prime_partial	59	147.8	sp\|Q9QYG0\|NDRG2_MOUSE 96.2% — Protein NDRG2
YWHAE `transcript1349.11.nic`	ORF disrupted	Lost: Ta0938	5prime_partial	44	110.2	sp\|P62261\|1433E_BOVIN 100.0% — 14-3-3 protein epsilon
Cops7a `transcript1298.6.nic`	ORF disrupted		5prime_partial	42	105.2	sp\|Q9CZ04\|CSN7A_MOUSE 100.0% — COP9 signalosome complex subun...
Stoml2 `transcript343.4.nic`	ORF disrupted		5prime_partial	42	105.2	sp\|Q99JB2\|STML2_MOUSE 100.0% — Stomatin-like protein 2, mitoc...
Ntan1 `transcript180.16.nic`	ORF disrupted		5prime_partial	25	62.6	sp\|Q64311\|NTAN1_MOUSE 100.0% — Protein N-terminal asparagine ...
Stx4 `transcript2593.7.nic`	ORF disrupted	Lost: COG2, Csm1_N, DUF3671, DUF3698, E-set, ETRAMP, His_Kinase_A, PRP1_N, T2SSF, TPR, TPR_MLP1_2, Vac_Fusion Gained: GOLD-like	3prime_partial	22	55.1	sp\|P70452\|STX4_MOUSE 100.0% — Syntaxin-4
Cryzl1 `transcript1013.16.nic`	ORF disrupted		5prime_partial	21	52.6	sp\|Q921W4\|QORL1_MOUSE 100.0% — Quinone oxidoreductase-like pr...
Slc5a5 `transcript757.8.nic`	ORF disrupted		5prime_partial	19	47.6	sp\|Q99PN0\|SC5A5_MOUSE 99.4% — Sodium/iodide cotransporter
Aprt `transcript1695.8.nic`	ORF disrupted		5prime_partial	17	42.6	sp\|P08030\|APT_MOUSE 97.5% — Adenine phosphoribosyltransfer...
Sparcl1 `transcript878.5.nic`	ORF disrupted		3prime_partial	15	37.6	sp\|P70663\|SPRL1_MOUSE 100.0% — SPARC-like protein 1
P33monox `transcript479.13.nic`	ORF disrupted		5prime_partial	14	35.1	sp\|Q9DBN4\|P33MX_MOUSE 100.0% — Putative monooxygenase p33MONO...
YPEL3 `transcript2436.7.nic`	ORF disrupted		5prime_partial	12	30.1	sp\|A6QPH8\|YPEL3_BOVIN 100.0% — Protein yippee-like 3
Itm2c `transcript737.1.nnic`	ORF disrupted	Lost: Brichos_S Gained: EF_hand	internal	12	30.1	—
Smyd3 `transcript1604.1.nic`	ORF disrupted		5prime_partial	11	27.6	sp\|Q9CWR2\|SMYD3_MOUSE 100.0% — Histone-lysine N-methyltransfe...
Cuedc2 `transcript1066.19.nnic`	ORF disrupted		3prime_partial	10	25.0	sp\|Q9CXX9\|CUED2_MOUSE 91.6% — CUE domain-containing protein ...
Mobp `transcript1966.9.nic`	ORF disrupted		5prime_partial	10	25.0	sp\|Q9D2P8\|MOBP_MOUSE 100.0% — Myelin-associated oligodendroc...
Yif1b `transcript833.7.nic`	ORF disrupted		5prime_partial	10	25.0	sp\|Q9CX30\|YIF1B_MOUSE 95.5% — Protein YIF1B
Emg1 `transcript1182.6.nic`	ORF disrupted	Gained: MIF	5prime_partial	9	22.5	sp\|O35130\|NEP1_MOUSE 100.0% — Ribosomal RNA small subunit me...
Ntan1 `transcript186.16.nic`	ORF disrupted		5prime_partial	9	22.5	sp\|Q64311\|NTAN1_MOUSE 83.3% — Protein N-terminal asparagine ...
Drap1 `transcript208.19.nnic`	ORF disrupted	Lost: EBV-NA3, FXMRP1_C_core, Histone, PPP4R2 Gained: Secretin_N_2	5prime_partial	9	22.5	sp\|Q9D6N5\|NC2A_MOUSE 100.0% — Dr1-associated corepressor
Rgs10 `transcript2659.7.nnic`	ORF disrupted		5prime_partial	9	22.5	sp\|Q9CQE5\|RGS10_MOUSE 100.0% — Regulator of G-protein signali...
Septin4 `transcript1641.11.nic`	ORF disrupted		5prime_partial	8	20.0	sp\|P28661\|SEPT4_MOUSE 99.6% — Septin-4
Fkbp2 `transcript474.19.nnic`	ORF disrupted		5prime_partial	8	20.0	sp\|P45878\|FKBP2_MOUSE 100.0% — Peptidyl-prolyl cis-trans isom...
Rtkn `transcript665.6.nic`	ORF disrupted		5prime_partial	8	20.0	sp\|Q8C6B2\|RTKN_MOUSE 95.5% — Rhotekin
Lsm7 `transcript844.10.nic`	ORF disrupted	Lost: SH3 Gained: ExoD, TPR	5prime_partial	8	20.0	—
Cuedc2 `transcript1068.19.nnic`	ORF disrupted		3prime_partial	7	17.5	sp\|Q9CXX9\|CUED2_MOUSE 99.2% — CUE domain-containing protein ...
Sys1 `transcript2436.2.nic`	ORF disrupted		5prime_partial	7	17.5	sp\|Q78S06\|SYS1_MOUSE 100.0% — Protein SYS1 homolog
Bcas1 `transcript2552.2.nnic`	ORF disrupted		5prime_partial	7	17.5	sp\|Q80YN3\|BCAS1_MOUSE 85.3% — Breast carcinoma-amplified seq...
TXNL4A `transcript762.18.nnic`	ORF disrupted		3prime_partial	7	17.5	—
Hpcal1 `transcript118.12.nnic`	ORF disrupted		3prime_partial	6	15.0	sp\|P62748\|HPCL1_MOUSE 100.0% — Hippocalcin-like protein 1
H2AZ1 `transcript1247.3.nic`	ORF disrupted		5prime_partial	6	15.0	sp\|P0C0S4\|H2AZ_BOVIN 100.0% — Histone H2A.Z
Me3 `transcript1768.7.nic`	ORF disrupted	Lost: NADP_Rossmann	5prime_partial	6	15.0	sp\|Q8BMF3\|MAON_MOUSE 100.0% — NADP-dependent malic enzyme, m...
Ly6h `transcript423.15.nnic`	ORF disrupted		5prime_partial	6	15.0	sp\|Q9WUC3\|LY6H_MOUSE 100.0% — Lymphocyte antigen 6H
Tmem198 `transcript639.1.nic`	ORF disrupted		3prime_partial	6	15.0	sp\|Q8BG75\|TM198_MOUSE 100.0% — Transmembrane protein 198
Ckmt1 `transcript1482.2.nnic`	ORF disrupted	Lost: ATP-gua_PtransN	5prime_partial	5	12.5	sp\|P30275\|KCRU_MOUSE 100.0% — Creatine kinase U-type, mitoch...
Mrps17 `transcript1487.5.nnic`	ORF disrupted		5prime_partial	5	12.5	sp\|Q9CQE3\|RT17_MOUSE 100.0% — Small ribosomal subunit protei...
Reep5 `transcript226.18.nic`	ORF disrupted	Lost: TB2_DP1_HVA22	internal	5	12.5	sp\|Q00765\|REEP5_HUMAN 100.0% — Receptor expression-enhancing ...
Comt `transcript372.16.nnic`	ORF disrupted	Lost: CmcI, NADP_Rossmann	5prime_partial	5	12.5	sp\|O88587\|COMT_MOUSE 100.0% — Catechol O-methyltransferase
R3hdm4 `transcript707.10.nic`	ORF disrupted		5prime_partial	5	12.5	sp\|Q4VBF2\|R3HD4_MOUSE 99.5% — R3H domain-containing protein ...
Rbp4 `transcript896.19.nic`	ORF disrupted		5prime_partial	5	12.5	sp\|Q00724\|RET4_MOUSE 100.0% — Retinol-binding protein 4
Ppt1 `transcript947.4.nnic`	ORF disrupted	Lost: U71	3prime_partial	5	12.5	sp\|O88531\|PPT1_MOUSE 100.0% — Palmitoyl-protein thioesterase...
Amn `transcript982.12.nic`	ORF disrupted		5prime_partial	5	12.5	sp\|Q99JB7\|AMNLS_MOUSE 100.0% — Protein amnionless
Ak6 `transcript1017.13.nic`	ORF disrupted	Lost: AAA_lid, DUF2125, DUF3245, DUF4611, Histone	5prime_partial	4	10.0	sp\|Q8VCP8\|KAD6_MOUSE 100.0% — Adenylate kinase isoenzyme 6
KLK6 `transcript1120.7.nic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q92876\|KLK6_HUMAN 68.4% — Kallikrein-6
Gtf2a2 `transcript1179.9.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q80ZM7\|T2AG_MOUSE 100.0% — Transcription initiation facto...
LMO4 `transcript1282.3.nnic`	ORF disrupted	Lost: TRASH	5prime_partial	4	10.0	sp\|Q3SWZ8\|LMO4_BOVIN 100.0% — LIM domain transcription facto...
Ntan1 `transcript187.16.nnic`	ORF disrupted	Lost: FIST	5prime_partial	4	10.0	sp\|Q64311\|NTAN1_MOUSE 91.6% — Protein N-terminal asparagine ...
Exosc7 `transcript2042.9.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q9D0M0\|EXOS7_MOUSE 91.7% — Exosome complex exonuclease RR...
Raly `transcript2185.2.nic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q64012\|RALY_MOUSE 94.9% — RNA-binding protein Raly
Dusp26 `transcript244.8.nic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q9D700\|DUS26_MOUSE 100.0% — Dual specificity protein phosp...
Phkg2 `transcript2577.7.nic`	ORF disrupted	Gained: FliG	3prime_partial	4	10.0	sp\|Q9DB30\|PHKG2_MOUSE 100.0% — Phosphorylase b kinase gamma c...
Mcts1 `transcript343.X.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q4G009\|MCTS1_RAT 100.0% — Malignant T-cell-amplified seq...
P33monox `transcript481.13.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q9DBN4\|P33MX_MOUSE 99.2% — Putative monooxygenase p33MONO...
Hapln4 `transcript536.8.nic`	ORF disrupted		3prime_partial	4	10.0	sp\|Q80WM4\|HPLN4_MOUSE 99.3% — Hyaluronan and proteoglycan li...
Ubald1 `transcript74.16.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q6P3B2\|UBAD1_MOUSE 97.8% — UBA-like domain-containing pro...
Mindy3 `transcript80.2.nnic`	ORF disrupted	Lost: Peptidase_MA	5prime_partial	4	10.0	sp\|Q9CV28\|MINY3_MOUSE 94.4% — Ubiquitin carboxyl-terminal hy...
Izumo4 `transcript817.10.nic`	ORF disrupted		5prime_partial	4	10.0	sp\|D3Z690\|IZUM4_MOUSE 97.4% — Izumo sperm-egg fusion protein...
Ubl7 `transcript869.9.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q91W67\|UBL7_MOUSE 100.0% — Ubiquitin-like protein 7
Mrpl39 `transcript915.16.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q9JKF7\|RM39_MOUSE 87.7% — Large ribosomal subunit protei...
Mmaa `transcript915.8.nnic`	ORF disrupted		5prime_partial	4	10.0	sp\|Q8C7H1\|MMAA_MOUSE 100.0% — Methylmalonic aciduria type A ...
Meaf6 `transcript1015.4.nic`	ORF disrupted		3prime_partial	3	7.5	sp\|Q2VPQ9\|EAF6_MOUSE 96.0% — Chromatin modification-related...
Xrcc3 `transcript1105.12.nic`	ORF disrupted		5prime_partial	3	7.5	sp\|Q9CXE6\|XRCC3_MOUSE 99.7% — DNA repair protein XRCC3
Isoc2b `transcript116.7.nnic`	ORF disrupted		5prime_partial	3	7.5	sp\|Q9DCC7\|ISC2B_MOUSE 66.7% — Isochorismatase domain-contain...
Scg3 `transcript1281.9.nic`	ORF disrupted		5prime_partial	3	7.5	sp\|P47867\|SCG3_MOUSE 91.6% — Secretogranin-3
Ca11 `transcript1392.7.nic`	ORF disrupted		5prime_partial	3	7.5	—
Psph `transcript1493.5.nic`	ORF disrupted		5prime_partial	3	7.5	sp\|Q99LS3\|SERB_MOUSE 100.0% — Phosphoserine phosphatase

Frame Impact Classification

Functional Diversity per Gene

Top Pfam Domains

Domain Architectures per Gene

Annotation QC: Pfam Artifact Detection

Related Pfam families are grouped into clans; proteins with anomalously high domain density (structural protein artifacts) are flagged separately. The cleaned set is used for all downstream analysis.

Original DV Genes

789

After QC Filtering

566

Removed (Artifacts)

223

Flagged Details

116

Flagged Artifacts (116)

Gene	Gene Name	Reason	Arch (raw)	Arch (collapsed)	Core / Variable Domains
`ENSMUSG00000032366`	TPM4	density artifact	6	0	44 / 20
`ENSMUSG00000027940`	Tpm3	density artifact	5	0	41 / 17
`ENSMUSG00000026761`	Orc4	density artifact	3	0	43 / 12
`ENSMUSG00000014402`	Tsg101	density artifact	3	0	38 / 5
`ENSMUSG00000002985`	Apoe	density artifact	3	0	39 / 14
`ENSMUSG00000024983`	Vti1a	density artifact	2	0	27 / 0
`ENSMUSG00000007207`	Stx1a	density artifact	2	0	53 / 0
`ENSMUSG00000019303`	Psmc3ip	density artifact	2	0	71 / 0
`ENSMUSG00000059689`	Znf32	density artifact	2	0	40 / 0
`ENSMUSG00000027506`	Tpd52	density artifact	2	0	37 / 0
`ENSMUSG00000030603`	Psmc4	density artifact	2	0	38 / 0
`ENSMUSG00000031851`	Ntpcr	density artifact	2	0	41 / 0
`ENSMUSG00000069208`	Znf431	density artifact	2	0	29 / 0
`ENSMUSG00000026966`	Ssna1	density artifact	1	0	55 / 0
`ENSMUSG00000113621`	—	density artifact	1	0	35 / 0
`ENSMUSG00000129504`	Nefm	density artifact	1	0	31 / 0
`ENSMUSG00000042097`	Znf239	density artifact	1	0	31 / 0
`ENSMUSG00000020486`	Septin4	clan collapse	4	1	13 / 3
`ENSMUSG00000032112`	Trappc4	clan collapse	4	2	4 / 0
`ENSMUSG00000011658`	Fuz	clan collapse	3	1	3 / 0
`ENSMUSG00000018593`	Sparc	clan collapse	3	1	7 / 1
`ENSMUSG00000055720`	Ubl7	clan collapse	3	1	6 / 0
`ENSMUSG00000025478`	Dpysl4	clan collapse	2	1	3 / 0
`ENSMUSG00000024218`	Taf11	clan collapse	2	1	3 / 0
`ENSMUSG00000032786`	Alas1	clan collapse	2	1	5 / 0
`ENSMUSG00000037062`	Sh3glb1	clan collapse	2	1	7 / 0
`ENSMUSG00000024862`	KLC2	clan collapse	2	1	38 / 0
`ENSMUSG00000038671`	Arfrp1	clan collapse	2	1	9 / 0
`ENSMUSG00000034209`	Rasl10a	clan collapse	2	1	7 / 0
`ENSMUSG00000045302`	Preb	clan collapse	2	1	3 / 0
`ENSMUSG00000053483`	Usp21	clan collapse	2	1	3 / 0
`ENSMUSG00000020611`	Gna13	clan collapse	2	1	9 / 0
`ENSMUSG00000002524`	Puf60	clan collapse	2	1	5 / 0
`ENSMUSG00000036775`	Decr2	clan collapse	2	1	5 / 0
`ENSMUSG00000047067`	Dusp28	clan collapse	2	1	4 / 0
`ENSMUSG00000116594`	SEPTIN5	clan collapse	2	1	16 / 0
`ENSMUSG00000031812`	Map1lc3b	clan collapse	2	1	3 / 0
`ENSMUSG00000024576`	CSNK1A1	clan collapse	2	1	7 / 0
`ENSMUSG00000032320`	Rcn2	clan collapse	2	1	5 / 0
`ENSMUSG00000042831`	Alkbh6	clan collapse	2	1	1 / 1
`ENSMUSG00000017307`	Acot8	clan collapse	2	1	3 / 0
`ENSMUSG00000039183`	Nubp2	clan collapse	2	1	2 / 19
`ENSMUSG00000021287`	Xrcc3	clan collapse	2	1	6 / 0
`ENSMUSG00000004032`	Gstm5	clan collapse	2	1	6 / 0
`ENSMUSG00000031505`	Naxd	clan collapse	2	1	3 / 0
`ENSMUSG00000020873`	Slc35b1	clan collapse	2	1	5 / 0
`ENSMUSG00000039637`	Coro7	clan collapse	2	1	10 / 0
`ENSMUSG00000019173`	Rab5c	clan collapse	2	1	9 / 0
`ENSMUSG00000000184`	Ccnd2	clan collapse	2	1	1 / 2
`ENSMUSG00000019295`	Tmem129	clan collapse	2	1	4 / 0
`ENSMUSG00000056367`	Actr3b	clan collapse	2	1	5 / 0
`ENSMUSG00000022793`	B4galt4	clan collapse	2	1	3 / 0
`ENSMUSG00000021548`	Ccnh	clan collapse	2	1	3 / 0
`ENSMUSG00000030386`	ZNF606	clan collapse	2	1	16 / 0
`ENSMUSG00000028099`	Polr3c	clan collapse	2	1	3 / 0
`ENSMUSG00000026632`	Tatdn3	clan collapse	2	1	1 / 1
`ENSMUSG00000059248`	Septin9	clan collapse	2	1	29 / 0
`ENSMUSG00000026201`	Stk16	clan collapse	2	1	7 / 1
`ENSMUSG00000063065`	Mapk3	clan collapse	2	1	9 / 0
`ENSMUSG00000037022`	Mmaa	clan collapse	2	1	14 / 0
`ENSMUSG00000087260`	Lamtor5	clan collapse	2	1	2 / 0
`ENSMUSG00000072214`	Septin5	clan collapse	2	1	14 / 0
`ENSMUSG00000021773`	Comtd1	clan collapse	2	1	7 / 0
`ENSMUSG00000046434`	HNRNPA1	clan collapse	2	1	6 / 0
`ENSMUSG00000038975`	Rabggtb	clan collapse	2	1	3 / 0
`ENSMUSG00000029534`	ST7	clan collapse	2	1	8 / 0
`ENSMUSG00000020794`	UBE2G1	clan collapse	2	1	2 / 0
`ENSMUSG00000027998`	Plrg1	clan collapse	2	1	9 / 0
`ENSMUSG00000052752`	TRAF7	clan collapse	2	1	24 / 1
`ENSMUSG00000021024`	Psma6	clan collapse	2	1	2 / 0
`ENSMUSG00000019977`	Hbs1l	clan collapse	2	1	8 / 0
`ENSMUSG00000026034`	Clk1	clan collapse	2	1	3 / 0
`ENSMUSG00000022674`	Ube2v2	clan collapse	2	1	2 / 0
`ENSMUSG00000049225`	Pdp1	clan collapse	2	1	1 / 1
`ENSMUSG00000021772`	Nkiras1	clan collapse	2	1	9 / 0
`ENSMUSG00000029203`	UBE2K	clan collapse	2	1	6 / 0
`ENSMUSG00000057411`	Antkmt	clan collapse	2	1	4 / 1
`ENSMUSG00000027176`	Cstf3	clan collapse	2	1	8 / 0
`ENSMUSG00000037373`	Ctbp1	clan collapse	2	1	6 / 0
`ENSMUSG00000047766`	Lrrc49	clan collapse	2	1	4 / 0
`ENSMUSG00000030701`	Plekhb1	clan collapse	2	1	2 / 1
`ENSMUSG00000033429`	Mcee	clan collapse	2	1	5 / 0
`ENSMUSG00000031570`	Plpp5	clan collapse	2	1	4 / 0
`ENSMUSG00000042540`	Acot5	clan collapse	2	1	5 / 0
`ENSMUSG00000022635`	Zcrb1	clan collapse	2	1	6 / 0
`ENSMUSG00000027679`	Dnajc19	clan collapse	2	1	2 / 0
`ENSMUSG00000064068`	Mtx1	clan collapse	2	1	5 / 0
`ENSMUSG00000020385`	Clk4	clan collapse	2	1	5 / 0
`ENSMUSG00000006782`	Cnp	clan collapse	2	1	23 / 0
`ENSMUSG00000030512`	SNRPA1	clan collapse	2	1	3 / 1
`ENSMUSG00000030272`	Camk1	clan collapse	2	1	9 / 0
`ENSMUSG00000058240`	Cryzl1	clan collapse	2	1	2 / 1
`ENSMUSG00000022971`	Ifnar2	clan collapse	2	1	4 / 0
`ENSMUSG00000000253`	Gmpr	clan collapse	2	1	4 / 0
`ENSMUSG00000022474`	Pmm1	clan collapse	2	1	3 / 0
`ENSMUSG00000038286`	Bphl	clan collapse	2	1	12 / 0
`ENSMUSG00000023755`	Rhebl1	clan collapse	2	1	8 / 0
`ENSMUSG00000051730`	Mettl5	clan collapse	2	1	23 / 0
`ENSMUSG00000007594`	Hapln4	clan collapse	2	1	7 / 0
`ENSMUSG00000005150`	Wdr83	clan collapse	2	1	4 / 2
`ENSMUSG00000055409`	Nell1	clan collapse	2	1	13 / 0
`ENSMUSG00000025731`	Mettl26	clan collapse	2	1	6 / 1
`ENSMUSG00000032336`	Nptn	clan collapse	2	1	8 / 2
`ENSMUSG00000078517`	Emc1	clan collapse	2	1	3 / 0
`ENSMUSG00000029538`	Srsf9	clan collapse	2	1	5 / 0
`ENSMUSG00000044628`	Rnf208	clan collapse	2	1	10 / 0
`ENSMUSG00000045136`	TUBB2B	clan collapse	2	1	3 / 0
`ENSMUSG00000025939`	Ube2w	clan collapse	2	1	3 / 0
`ENSMUSG00000019054`	Fis1	clan collapse	2	1	8 / 0
`ENSMUSG00000024516`	Sec11c	clan collapse	2	1	2 / 0
`ENSMUSG00000025794`	Rpl14	clan collapse	2	1	3 / 0
`ENSMUSG00000040532`	Abhd11	clan collapse	2	1	14 / 0
`ENSMUSG00000023175`	Bsg	clan collapse	2	1	7 / 0
`ENSMUSG00000058291`	Zfp39	clan collapse	2	1	9 / 1
`ENSMUSG00000078853`	Igtp	clan collapse	2	1	6 / 0
`ENSMUSG00000013622`	Atraid	clan collapse	2	1	4 / 0

Domain Variants by Evidence Tier

Variable Domain Landscape

Total Variable Domains

351

Domain Loss Events

360

Domain Gain Events

Loss : Gain Ratio

17.1:1

Domain loss dominates gain ~17:1, consistent with alternative splicing predominantly removing exons encoding specific domains rather than inserting novel domain-encoding exons.

All Variable Domains (351)

Domain	DV Genes	Gain	Loss	Genes
`AAA_25`	2	0	2	Ak6, Pstk
`AAA_16`	2	0	2	Ak6, Pstk
`TPR_MLP1_2`	2	0	2	Fgfr1op2, Stx4
`Csm1_N`	2	0	2	Olfm1, Stx4
`SlyX`	2	0	2	Egfl7, Olfm1
`Fib_alpha`	2	0	2	Olfm1, Tpd52l1
`PPP4R2`	2	1	1	Drap1, Apbb1
`Occludin_ELL`	2	0	2	Fgfr1op2, Olfm1
`DUF4446`	2	0	2	Fgfr1op2, Olfm1
`UPF0242`	2	0	2	Fgfr1op2, Gabbr1
`Sec20`	2	0	2	Fgfr1op2, Tmem191
`ZapB`	2	0	2	Fgfr1op2, Olfm1
`Ge1_WD40`	2	0	2	Fbxw2, Poc1a
`DUF1664`	2	0	2	Bin1, Olfm1
`Jnk-SapK_ap_N`	2	0	2	Bin1, Stx5
`Exonuc_VII_L`	2	0	2	Olfm1, Septin8
`ATG16`	2	0	2	Olfm1, Septin8
`ubiquitin`	2	0	2	Kxd1, Tecr
`TMPIT`	2	0	2	Hnrnpc, Olfm1
`Proteasome_A_N`	2	0	2	Poc1a, Psma3
`Pkinase_fungal`	2	1	1	Pak1, Araf
`APH`	2	1	1	Pak1, Araf
`ABC1`	2	1	1	Pak1, Araf
`Kdo`	2	1	1	Pak1, Araf
`PK_Tyr_Ser-Thr`	2	0	2	Araf, Pak1
`Pkinase`	2	0	2	Araf, Pak1
`Kinase-like`	2	1	1	Pak1, Araf
`NPV_P10`	2	0	2	Olfm1, Tmem191
`Syntaxin_2`	2	0	2	Olfm1, Stx5
`DUF6161`	2	0	2	CLTA, Cltb
`DUF6076`	1	0	1	ZNF286A
`zinc_ribbon_9`	1	0	1	ZNF286A
`DUF5830`	1	0	1	ZNF286A
`zf-LYAR`	1	0	1	ZNF286A
`GlcNAc-1_reg`	1	0	1	Tcp1
`RE_HindIII`	1	0	1	Exog
`G6B`	1	0	1	Ccdc106
`CDC24`	1	0	1	Aplp2
`APP_amyloid`	1	0	1	Aplp2
`RapH_N`	1	0	1	Aplp2
`DUF4748`	1	0	1	Pnkd
`dsrm`	1	0	1	Son
`DND1_DSRM`	1	0	1	Son
`G-patch`	1	0	1	Son
`G-patch_2`	1	0	1	Son
`IQ`	1	0	1	Gap43
`Neuromodulin_N`	1	0	1	Gap43
`Neuromodulin`	1	0	1	Gap43
`Het-C`	1	0	1	Sgce
`PRR20`	1	0	1	Fam241b
`Phage_glycop_gL`	1	0	1	Agpat3
`zf-C2H2_8`	1	0	1	ZNF707
`Zn_ribbon_recom`	1	0	1	ZNF688
`TFIIS_C`	1	0	1	ZNF688
`zf-C2H2_jaz`	1	0	1	ZNF688
`zf_UBZ`	1	0	1	ZNF688
`Vps36-NZF-N`	1	0	1	ZNF688
`zf-ISL3`	1	0	1	ZNF688
`HTH_20`	1	0	1	Myl4
`AAA_24`	1	0	1	Ak6
`DUF4611`	1	0	1	Ak6
`AAA_14`	1	0	1	Ak6
`DUF2125`	1	0	1	Ak6
`NACHT`	1	0	1	Ak6
`DUF3245`	1	0	1	Ak6
`CENP-S`	1	0	1	Ak6
`TFIID-31kDa`	1	0	1	Ak6
`KTI12`	1	0	1	Ak6
`IstB_IS21`	1	0	1	Ak6
`ATPase`	1	0	1	Ak6
`AAA_3`	1	0	1	Ak6
`AAA_5`	1	0	1	Ak6
`Histone`	1	0	1	Ak6
`PduV-EutP`	1	0	1	Ak6
`RuvB_N`	1	0	1	Ak6
`Mg_chelatase`	1	0	1	Ak6
`Bromo_TP`	1	0	1	Ak6
`LonB_AAA-LID`	1	0	1	Ak6
`NTPase_1`	1	0	1	Ak6
`Thymidylate_kin`	1	0	1	Ak6
`AAA_30`	1	0	1	Ak6
`TsaE`	1	0	1	Ak6
`CENP-T_C`	1	0	1	Ak6
`Herpes_DNAp_acc`	1	0	1	Eef1d
`Cytochrom_C_2`	1	0	1	Eef1d
`zf-RRN7`	1	0	1	Mical2
`zf-C3HC4_2`	1	0	1	Mical2
`TNFR_c6`	1	0	1	Mical2
`DUF3742`	1	0	1	Atp6v0b
`bZIP_Maf`	1	0	1	Sh3glb2
`Fmp27_WPPW`	1	0	1	Chmp2a
`Ku_C`	1	0	1	Chmp2a
`ISG65-75`	1	0	1	Chmp2a
`HrcA`	1	0	1	Chmp2a
`Mo25`	1	0	1	Chmp2a
`DUF1002`	1	0	1	Chmp2a
`HCV_NS5a_C`	1	0	1	Scamp3
`S_4TM`	1	0	1	Scamp3
`ETRAMP`	1	0	1	Stx4
`DUF3671`	1	0	1	Stx4
`Vac_Fusion`	1	0	1	Stx4
`HisKA_3`	1	0	1	Stx4
`EMP24_GP25L`	1	0	1	Stx4
`COG2`	1	0	1	Stx4
`T2SSF`	1	0	1	Stx4
`PRP1_N`	1	0	1	Stx4
`DUF6584`	1	0	1	Stx4
`Psg1`	1	0	1	Stx4
`RR_TM4-6`	1	0	1	Zcchc17
`BAR_2`	1	0	1	Arfip2
`DUF6288`	1	0	1	Nherf2
`DUF1450`	1	0	1	Nherf2
`Abhydrolase_1`	1	0	1	Ppt1
`U71`	1	0	1	Ppt1
`CtnDOT_TraJ`	1	0	1	Tpd52l1
`WEMBL`	1	0	1	Tpd52l1
`Uso1_p115_C`	1	0	1	Tpd52l1
`F-box-like`	1	0	1	Fbxl6
`F-box`	1	0	1	Fbxl6
`DNA_pol_phi`	1	1	0	Apbb1
`TPR_17`	1	0	1	Anapc5
`FixQ`	1	0	1	HNRNPR
`DUF6498`	1	0	1	HNRNPR
`Ribophorin_I`	1	0	1	Rpusd1
`SR1P`	1	0	1	Stx18
`TolA_bind_tri`	1	0	1	Fgfr1op2
`Phage_GP20`	1	0	1	Fgfr1op2
`LUC7`	1	0	1	Fgfr1op2
`FIN1`	1	0	1	Fgfr1op2
`WXG100`	1	0	1	Fgfr1op2
`CHD5`	1	0	1	Fgfr1op2
`Prefoldin_2`	1	0	1	Fgfr1op2
`betaPIX_CC`	1	0	1	Fgfr1op2
`Snapin_Pallidin`	1	0	1	Fgfr1op2
`DUF2570`	1	0	1	Fgfr1op2
`bZIP_1`	1	0	1	Fgfr1op2
`MAT1`	1	0	1	Fgfr1op2
`Cauli_AT`	1	0	1	Fgfr1op2
`YL1`	1	0	1	Npm1
`CHAP`	1	0	1	Fbxw2
`Lipoprotein_21`	1	0	1	Ube2v1
`BAR_3`	1	0	1	Bin1
`CK2S`	1	0	1	Bin1
`V-SNARE`	1	0	1	Bin1
`Arfaptin`	1	0	1	Bin1
`TBD`	1	0	1	Bin1
`OmpH`	1	0	1	Bin1
`DUF4519`	1	0	1	Lrrtm1
`Tudor-knot`	1	0	1	Lrrtm1
`LptF_LptG`	1	0	1	Lrrtm1
`Fucokinase`	1	0	1	Dctn6
`DUF3489`	1	0	1	Rpl13a
`Lsm_interact`	1	0	1	Gramd1a
`MHYT`	1	0	1	Gramd1a
`Sec34`	1	0	1	Exoc7
`Microtub_bd`	1	0	1	Septin8
`PX`	1	0	1	Sgk1
`DUF2946`	1	0	1	TSC22D1
`UPF0181`	1	0	1	Sf1
`Presenilin`	1	0	1	RNPS1
`Pyr_redox_dim`	1	0	1	Mrpl28
`Herpes_capsid`	1	0	1	Dynlt1
`WIF`	1	0	1	Lgals8
`HSP70`	1	0	1	Kars1
`XRN1_DBM`	1	0	1	Tmub2
`Redoxin`	1	0	1	Rpl34
`DUF4736`	1	0	1	Rtn1
`PhageMetallopep`	1	1	0	Mindy3
`Cytadhesin_P30`	1	0	1	Hnrnpc
`Nup88`	1	0	1	Hnrnpc
`DUF4407`	1	1	0	Hnrnpc
`PA26`	1	1	0	Hnrnpc
`PCAF_N`	1	0	1	Pea15
`WD40_like`	1	0	1	Poc1a
`Cytochrom_D1`	1	0	1	Poc1a
`Nup160`	1	0	1	Poc1a
`ANAPC4_WD40`	1	0	1	Poc1a
`eIF2A`	1	0	1	Poc1a
`NBCH_WD40`	1	0	1	Poc1a
`DUF6304`	1	0	1	Rundc3a
`WT1`	1	0	1	Rundc3a
`SNF2-rel_dom`	1	0	1	Ddx24
`TF_Zn_Ribbon`	1	0	1	Pdlim7
`LIM`	1	0	1	Pdlim7
`DZR`	1	0	1	Pdlim7
`zf-ribbon_3`	1	0	1	Pdlim7
`FliG_N`	1	0	1	Phkg2
`FTA2`	1	0	1	Phkg2
`DUF2112`	1	0	1	Ttc33
`zf-RanBP`	1	0	1	Ttc33
`CCD97-like_C`	1	0	1	Ttc33
`GEN1_C`	1	0	1	Nap1l1
`NPL`	1	0	1	Nap1l1
`CBF`	1	0	1	Nap1l1
`Lgl_C`	1	0	1	Dda1
`DUF3138`	1	0	1	Dda1
`cobW`	1	0	1	Gtpbp6
`AIRS`	1	0	1	Pak1
`DUF3741`	1	0	1	Pak1
`DSHCT`	1	0	1	Pak1
`DUF2813`	1	0	1	Pak1
`MASE3`	1	0	1	Hm13
`ROK`	1	0	1	Nagk
`DUF745`	1	0	1	Tmem191
`pRN1_helical`	1	0	1	Tmem191
`Thump_like`	1	0	1	Naa10
`PRKCSH`	1	0	1	Gnptg
`AltA1`	1	0	1	Gnptg
`PTN_MK_N`	1	0	1	Shisa5
`TMEM171`	1	0	1	Tmem134
`DUF6232`	1	1	0	Tmem134
`DPM3`	1	1	0	Tmem134
`ThrE_2`	1	1	0	Tmem134
`Virul_fac_BrkB`	1	1	0	Tmem134
`TBK1_ULD`	1	0	1	Kxd1
`Ribosomal_L40e`	1	0	1	Kxd1
`Ubiquitin_2`	1	0	1	Kxd1
`Sde2_N_Ubi`	1	0	1	Kxd1
`Rad60-SLD`	1	0	1	Kxd1
`Ubiquitin_5`	1	0	1	Kxd1
`DUF2604`	1	0	1	Kxd1
`Ubiquitin_4`	1	0	1	Kxd1
`Rad60-SLD_2`	1	0	1	Kxd1
`DUF4726`	1	0	1	Hspbp1
`FHIPEP`	1	0	1	Zwint
`Spectrin`	1	0	1	Olfm1
`FUSC`	1	0	1	Olfm1
`DUF5917`	1	0	1	Olfm1
`Csm2_III-A`	1	0	1	Olfm1
`PriC`	1	0	1	Olfm1
`Nup54`	1	0	1	Olfm1
`Enkurin`	1	0	1	Olfm1
`HSBP1`	1	0	1	Olfm1
`ATG17_like`	1	0	1	Olfm1
`Laminin_II`	1	0	1	Olfm1
`Baculo_PEP_C`	1	0	1	Olfm1
`FtsZ_C`	1	0	1	Olfm1
`DUF2203`	1	0	1	Olfm1
`PilJ`	1	0	1	Olfm1
`ABC_tran_CTD`	1	0	1	Olfm1
`IL2`	1	0	1	Olfm1
`Cytochrom_B562`	1	0	1	Olfm1
`Helo_like_N`	1	0	1	Olfm1
`H-kinase_dim`	1	0	1	Olfm1
`Myosin_tail_1`	1	0	1	Olfm1
`EzrA`	1	0	1	Olfm1
`FapA`	1	0	1	Olfm1
`OLF`	1	0	1	Olfm1
`TBCC_N`	1	0	1	Olfm1
`FAM184`	1	0	1	CLTA
`LapA_dom`	1	0	1	Gpm6b
`Amidase`	1	0	1	Pja1
`DNA_primase_lrg`	1	0	1	Rpl18a
`CDP-OH_P_tran_2`	1	0	1	Mtch2
`COG7`	1	0	1	Lsm7
`ExoD`	1	0	1	Lsm7
`YopD`	1	0	1	Fam107a
`AAA_13`	1	0	1	Tpd52l2
`DUF4164`	1	1	0	Tpd52l2
`Glyco_hydro_36`	1	1	0	Tpd52l2
`Ank_4`	1	0	1	Acbd6
`Ank_5`	1	0	1	Acbd6
`Ank_3`	1	0	1	Acbd6
`Ank_2`	1	0	1	Acbd6
`Ank`	1	0	1	Acbd6
`Herpes_LMP1`	1	0	1	Sirt5
`Tfb5`	1	0	1	Ndufab1
`WASH_WAHD`	1	0	1	Stx5
`QWRF`	1	0	1	Stx5
`Prominin`	1	0	1	Stx5
`Nuf2_DHR10-like`	1	0	1	Stx5
`Baculo_F`	1	0	1	Stx5
`DUF4795`	1	0	1	Stx5
`Rubredoxin_2`	1	0	1	Araf
`BBP1_C`	1	0	1	Uchl5
`Rep_fac_C`	1	0	1	Uchl5
`S-methyl_trans`	1	0	1	Uchl5
`AAR2`	1	0	1	Use1
`CcmH`	1	0	1	SMIM29
`DUF2371`	1	0	1	SMIM29
`PaREP1`	1	0	1	SMIM29
`COX14`	1	0	1	SMIM29
`INSIG`	1	0	1	Retreg2
`DUF3911`	1	0	1	Paip2
`Yip1`	1	0	1	Tusc3
`Bax1-I`	1	0	1	Tusc3
`YhhN`	1	0	1	Tusc3
`DUF6350`	1	0	1	Tusc3
`AAA_23`	1	0	1	Stmn4
`CCDC84`	1	0	1	Stmn4
`GBP_C`	1	0	1	Stmn4
`Kei1`	1	0	1	Gabbr1
`COX1`	1	0	1	Gabbr1
`CCDC-167`	1	0	1	Gabbr1
`Peripla_BP_6`	1	0	1	Gabbr1
`PKcGMP_CC`	1	0	1	Gabbr1
`7tm_3`	1	0	1	Gabbr1
`NEMP`	1	0	1	Gabbr1
`DUF3995`	1	0	1	Gabbr1
`ANF_receptor`	1	0	1	Gabbr1
`DUF6377`	1	0	1	Gabbr1
`AAA_18`	1	0	1	Pstk
`AAA_33`	1	0	1	Pstk
`AAA_22`	1	0	1	Pstk
`DEAD_assoc`	1	0	1	Pstk
`Zeta_toxin`	1	0	1	Pstk
`APS_kinase`	1	0	1	Pstk
`MeaB`	1	0	1	Pstk
`DO-GTPase1`	1	0	1	Pstk
`ParA`	1	0	1	Pstk
`FSA_C`	1	0	1	Khdrbs3
`Nup35_RRM_2`	1	0	1	HNRNPA2B1
`MIT`	1	0	1	Klc1
`TMF_TATA_bd`	1	0	1	Klc1
`IspA`	1	0	1	Dnajc7
`TPR-S`	1	0	1	Dnajc7
`CCER1`	1	0	1	Dnajc7
`LMBR1`	1	1	0	Psma3
`Amidohydro_2`	1	0	1	Nme3
`Peptidase_S77`	1	0	1	Nme3
`PglD_N`	1	0	1	H-RAS
`VirE`	1	0	1	H-RAS
`DiS_P_DiS`	1	0	1	YPEL3
`Elf1`	1	0	1	YPEL3
`LCD1`	1	1	0	Thyn1
`C2-set`	1	0	1	CD33
`HHH_8`	1	0	1	Polb
`WSN`	1	0	1	Polb
`Cdd1`	1	0	1	Polb
`HHH_5`	1	0	1	Polb
`HHH_2`	1	0	1	Polb
`HHH`	1	0	1	Polb
`HHH_3`	1	0	1	Polb
`DUF3701`	1	0	1	Polb
`IMS_HHH`	1	0	1	Polb
`DNA_pol_lambd_f`	1	0	1	Polb
`PRMT5`	1	0	1	Qdpr
`Epimerase`	1	0	1	Qdpr
`LppC`	1	0	1	Qdpr
`zf-RING_10`	1	0	1	Mrps18b
`HTH_21`	1	0	1	Mrps18b
`DUF6493`	1	0	1	Mbd3
`ATP-gua_PtransN`	1	0	1	Ckmt1
`MGC-24`	1	0	1	Cadm1
`Mid2`	1	0	1	Cadm1
`Mucin`	1	0	1	Cadm1
`VP1_VP3`	1	0	1	Serbp1
`FXMRP1_C_core`	1	1	0	Drap1
`Secretin_N_2`	1	1	0	Drap1
`EBV-NA3`	1	1	0	Drap1
`DUF4116`	1	0	1	Lrrc57

Domain Variant Genes (566 genes, multiple architectures after QC)

All domain variant genes retaining multiple domain architectures after clan collapse and density artifact removal. Use the search box to filter by gene ID, name, function, or domain.

Showing 566 of 566 genes

Gene	Name	Function	Isoforms	Arch	Tier	CPM	Source	Core Domains
`ENSMUSG00000024914`	Drap1	Dr1-associated corepressor	5	5	1	415.7	Mixed	CBFD_NFYB_HMF, Histone, TAF4
`ENSMUSG00000021288`	Klc1	Kinesin light chain 1	4	4	1	390.7	Mixed	ANAPC3, COG2, DUF4919, DUF627, GHL5, GlfT2_domain3, JIP_LZII, PPR, PPR_2, RNPP_C, RPN7, SHNi-TPR, SOGA, TPR_1, TPR_10, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_4, TPR_7, TPR_8, TPR_MalT, YabA
`ENSMUSG00000024845`	Tmem134	Transmembrane protein 134	6	4	1	72.6	Ref	DUF2207, DUF3040, DUF308, Neurensin, SdpI, TMEM_230_134
`ENSMUSG00000000827`	Tpd52l2	Tumor protein D54	6	4	1	27.5	Mixed	HIP1_clath_bdg, TPD52, VASP_tetra
`ENSMUSG00000062753`	SMIM29	Small integral membrane protein 29	4	4	4	72.6	Mixed	ABC2_membrane_3, BCCT, DUF3267, DUF3619, DUF4245, Hol_Tox
`ENSMUSG00000028049`	Scamp3	Secretory carrier-associated membrane protein 3	3	3	1	115.2	Mixed	LicD, MIC19_MIC25, Phage_holin_3_6, SCAMP
`ENSMUSG00000030805`	Stx4	Syntaxin-4	3	3	1	35.1	Novel	AAA_13, ATG17_like, BLOC1_2, Baculo_PEP_C, COG5, DUF16, DUF1664, DUF3698, DUF5798, Desmo_N, ERp29, GlutR_dimer, Lectin_N, LuxT_C, NPV_P10, PilO, SNARE, Syntaxin, Syntaxin_2
`ENSMUSG00000028478`	CLTA	Clathrin light chain A	4	3	1	561.0	Ref	CENP-Q, Clathrin_lg_ch, ORC3_N
`ENSMUSG00000010110`	Stx5	Syntaxin-5	4	3	1	25.0	Ref	Apolipoprotein, BLOC1_2, Cep3, DUF16, DUF1664, DUF5063, DUF5082, Fib_alpha, IZUMO, LPP, MCPsignal, NPV_P10, SKA1, SNARE, Spectrin, Syntaxin, Syntaxin-5_N, Troponin, Vac_Fusion
`ENSMUSG00000047547`	Cltb	Clathrin light chain B	3	3	1	222.9	Mixed	CENP-Q, Clathrin_lg_ch, GED
`ENSMUSG00000047342`	ZNF286A	Zinc finger protein 286A	3	3	1	17.5	Ref	C1_4, DNA_RNApol_7kD, DZR, HVO_2753_ZBP, HalOD2, KRAB, LIM, OSTMP1, TFIIS_C, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-met, zf-trcl, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000031996`	Aplp2	Amyloid beta precursor like protein 2	3	3	1	142.7	Novel	APP_Cu_bd, APP_E2, APP_N, Activator-TraM, CDC45, DabA, Kunitz_BPTI, VHL_C
`ENSMUSG00000045251`	ZNF688	Zinc finger protein 688	3	3	1	22.5	Ref	FYVE_2, KRAB, OrfB_Zn_ribbon, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-H2C2_2, zf-LYAR, zf-met, zinc_ribbon_9
`ENSMUSG00000033379`	Atp6v0b	V-type proton ATPase 21 kDa proteolipid subunit c''	4	3	1	1,139.5	Mixed	ATP-synt_C, G0-G1_switch_2, PIRT, YbgT_YccB
`ENSMUSG00000033916`	Chmp2a	Charged multivesicular body protein 2a	4	3	1	515.9	Ref	FapA, Snf7, YlqD
`ENSMUSG00000028772`	Zcchc17	Zinc finger CCHC domain-containing protein 17	4	3	1	20.0	Novel	CDC45, Coilin_N, CpXC, DUF3827, DUF4604, Hid1, Menin, S1, SR-25, zf-CCHC
`ENSMUSG00000022884`	EIF4A2	Eukaryotic initiation factor 4A-II	4	3	1	686.2	Ref	AAA_19, AAA_22, AAA_30, CMS1, DEAD, Fanconi_A, Flavi_DEAD, Helicase_C, Helicase_RecD, ResIII, UvrD-helicase, tRNA-synt_1b
`ENSMUSG00000040242`	Fgfr1op2	FGFR1 oncogene partner 2 homolog	3	3	1	35.1	Ref	Anthrax_toxA, DASH_Dam1, DUF16, Exonuc_VII_L, FUSC, FapA, Glyoxalase_8, Mgr1, NYD-SP28_assoc, PCRF, SIKE, TSC22, Tho2
`ENSMUSG00000024381`	Bin1	Myc box-dependent-interacting protein 1	3	3	1	80.1	Mixed	BAR, BLOC1_2, Baculo_PEP_C, DUF5667, DUF948, Laminin_II, PTS_IIA, SH3_1, SH3_2, SH3_9, THP2, YtxH
`ENSMUSG00000074129`	Rpl13a	Large ribosomal subunit protein uL13	4	3	1	2,649.6	Ref	Ribosomal_L13, SLT_L
`ENSMUSG00000001248`	Gramd1a	Protein Aster-A	3	3	1	17.5	Ref	GRAM, Pox_LP_H2, SMG1, VASt
`ENSMUSG00000022010`	TSC22D1	TSC22 domain family protein 1	5	3	1	568.5	Mixed	Chibby, DUF5660, DUF6262, DivIC, Fzo_mitofusin, K-box, SOGA, TSC22, YabA, ZapB, bZIP_1, bZIP_2
`ENSMUSG00000060373`	Hnrnpc	Heterogeneous nuclear ribonucleoproteins C1/C2	6	3	1	95.2	Ref	FUT8_N_cat, RRM_1, SKA1, Saf4_Yju2, XhlA, YabA
`ENSMUSG00000006575`	Rundc3a	RUN domain-containing protein 3A	3	3	1	147.8	Ref	DUF3450, DUF5595, Phage_GP20, RUN, UPF0242
`ENSMUSG00000021493`	Pdlim7	PDZ and LIM domain protein 7	4	3	1	130.2	Mixed	DUF4749, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Rv0078B
`ENSMUSG00000015126`	Tsr3	18S rRNA aminocarboxypropyltransferase	8	3	1	115.2	Ref	RLI, Ribo_biogen_C
`ENSMUSG00000058799`	Nap1l1	Nucleosome assembly protein 1-like 1	4	3	1	288.0	Novel	NAP
`ENSMUSG00000030774`	Pak1	Serine/threonine-protein kinase PAK 1	3	3	1	55.1	Novel	PBD
`ENSMUSG00000055692`	Tmem191	Transmembrane protein 191	3	3	1	40.1	Novel	CheZ, DUF1664, GAS, KASH_CCD, TMEM191C
`ENSMUSG00000035642`	Aamdc	Mth938 domain-containing protein	3	3	1	105.2	Ref	DUF498, Indigoidine_A, UPF0180
`ENSMUSG00000055553`	Kxd1	KxDL motif-containing protein 1	3	3	1	97.7	Mixed	KxDL, TolA_bind_tri
`ENSMUSG00000026833`	Olfm1	Noelin	3	3	1	741.3	Ref	BLOC1_2, CENP-Q, CLZ, DUF3450, Ephrin, MitMem_reg, Noelin-1, XhlA
`ENSMUSG00000031342`	Gpm6b	Neuronal membrane glycoprotein M6-b	3	3	1	25.0	Ref	DUF2755, DUF373, Myelin_PLP, PspB
`ENSMUSG00000001127`	Araf	Serine/threonine-protein kinase A-Raf	7	3	1	155.3	Mixed	C1_1, RBD, TUG-UBL1, zf-RING-like, zf-RING_2
`ENSMUSG00000018189`	Uchl5	Ubiquitin carboxyl-terminal hydrolase isozyme L5	3	3	1	37.6	Mixed	DUF2785, PNPase, Peptidase_C12, UCH_C
`ENSMUSG00000118664`	Tusc3	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3	3	3	1	50.1	Mixed	DUF4131, Gram_pos_anchor, OST3_OST6, Thioredoxin
`ENSMUSG00000022044`	Stmn4	Stathmin-4	4	3	1	538.4	Ref	ATG14, DHR-2_Lobe_C, EF-hand_14, Exonuc_VII_L, FUT8_N_cat, Pex19, Ribonuc_2-5A, Stathmin, UPF0242
`ENSMUSG00000022332`	Khdrbs3	KH domain-containing, RNA-binding, signal transduction-associated protein 3	3	3	1	62.6	Mixed	KH_1, Qua1, STAR_dimer, Sam68-YY
`ENSMUSG00000073435`	Nme3	Nucleoside diphosphate kinase 3	3	3	1	100.2	Mixed	NDK, PSI_PsaJ
`ENSMUSG00000032076`	Cadm1	Cell adhesion molecule 1	4	3	1	10.0	Mixed	Adeno_E3_CR1, Adhes-Ig_like, C1-set, C2-set, C2-set_2, DUF3439, I-set, Ig_2, Ig_3, Ig_4, Ig_C17orf99, Izumo-Ig, SSP160, Syndecan, V-set, ig
`ENSMUSG00000027286`	Lrrc57	Leucine-rich repeat-containing protein 57	4	3	1	62.6	Ref	CSTF_C, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9
`ENSMUSG00000034429`	ZNF707	Zinc finger protein 707	3	3	4	20.0	Ref	BolA, C1_4, CpXC, DpnI, HypA, KRAB, Zn-ribbon_8, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-LYAR, zf-RING_7, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000000296`	Tpd52l1	Tumor protein D53	3	3	4	17.5	Mixed	Cnn_1N, CorA, DUF1818, DUF3458_C, DUF745, NUDE_C, TMEM247, TPD52, Uds1
`ENSMUSG00000063179`	Pstk	L-seryl-tRNA(Sec) kinase	3	3	4	12.5	Novel	KTI12, LMBR1
`ENSMUSG00000073639`	Rab18	Ras-related protein Rab-18	2	2	1	12.5	Ref	AAA_16, AAA_22, AAA_24, AAA_29, AAA_7, ABC_tran, AIG1, ATP_bind_1, Arf, GTP_EFTU, Gtr1_RagA, Hpr_kinase_C, MMR_HSR1, NACHT, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB, Septin
`ENSMUSG00000007987`	Ift22	Intraflagellar transport protein 22 homolog	2	2	1	22.5	Ref	AAA, Arf, FMN_red, G-alpha, GTP_EFTU, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, SRPRB
`ENSMUSG00000001240`	Ramp2	Receptor activity-modifying protein 2	2	2	1	112.7	Ref	ExbD, RAMP
`ENSMUSG00000030881`	Arfip2	Arfaptin-2	3	2	1	52.6	Ref	Arfaptin, BAR, BAR_3, Blo-t-5, TPR_MLP1_2
`ENSMUSG00000029128`	Rab28	Ras-related protein Rab-28	2	2	1	50.1	Ref	Arf, DLIC, G-alpha, Gtr1_RagA, PduV-EutP, Ras, Roc, WHAMM-JMY_N, XFP_N
`ENSMUSG00000058569`	Tmed9	Transmembrane emp24 domain-containing protein 9	2	2	1	453.3	Novel	EMP24_GP25L, UPF0128, YfcL
`ENSMUSG00000029125`	Stx18	Syntaxin-18	3	2	1	20.0	Ref	DUF6279, Phage_CP76, Syntaxin-18_N, TipAS
`ENSMUSG00000030058`	Copg1	Coatomer subunit gamma-1	2	2	1	172.8	Ref	Adaptin_N, Arm, COP-gamma_platf, Cm_res_leader, Cnd1, Cnd3, Coatomer_g_Cpla, HEAT, HEAT_2, HEAT_EZ, HEAT_PBS, MOR2-PAG1_C, PDS5, UNC45-central
`ENSMUSG00000026797`	STXBP1	Syntaxin-binding protein 1	2	2	1	370.6	Ref	DUF5344, FRB_dom, Sec1, YlbD_coat
`ENSMUSG00000021486`	RAB24	Ras-related protein Rab-24	2	2	1	663.6	Ref	AAA_24, PRELI, Ras, Roc
`ENSMUSG00000034484`	Snx2	Sorting nexin-2	2	2	1	35.1	Novel	4HB_MCP_1, Allexi_40kDa, Arfaptin, BAR_3_WASP_bdg, BLOC1_2, DUF1870, DUF3775, DUF4618, DUF4795, Dynamin_N, Exonuc_VII_L, FAM92, FapA, Fez1, Fib_alpha, FliD_N, GrpE, HMMR_N, HR1, PX, Prefoldin_2, Sec34, Sorting_nexin, Vps5
`ENSMUSG00000030704`	Rab6a	Ras-related protein Rab-6A	2	2	1	20.0	Ref	Arf, CPT, FeoB_N, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB, TetR_C_16
`ENSMUSG00000048076`	ARF1	ADP-ribosylation factor 1	2	2	1	488.3	Ref	6PF2K, Arf, DO-GTPase2, DUF1806, G-alpha, Gtr1_RagA, MMR_HSR1, Ras, Roc, SRPRB
`ENSMUSG00000021686`	Ap3b1	AP-3 complex subunit beta-1	2	2	1	10.0	Ref	AP3B1_C, Adaptin_N, BUD22, Cnd1, DUF2457, HEAT, HEAT_2, HEAT_EZ, Macoilin, SEEEED, SRP-alpha_N
`ENSMUSG00000031539`	Ap3m2	AP-3 complex subunit mu-2	2	2	1	5.0	Ref	Adap_comp_sub, Clat_adaptor_s, DUF11
`ENSMUSG00000029146`	Snx17	Sorting nexin-17	2	2	1	90.2	Ref	Cation_ATPase_N, DUF2777, FERM_M, Nod1, PX, RA, Ras_bdg_2, SNX17_FERM_C
`ENSMUSG00000021124`	Vti1b	Vesicle transport through interaction with t-SNAREs homolog 1B	2	2	1	65.1	Ref	4HB_MCP_1, CCDC-167, Cir_Bir_Yir, DASH_Duo1, DUF1759, DUF3376, DUF3958, DUF6245, DUF745, Exonuc_VII_L, Fusion_gly, KxDL, Laminin_II, OmpH, Sec20, Snapin_Pallidin, Syntaxin-6_N, UPF0449, V-SNARE, V-SNARE_C, YqjK
`ENSMUSG00000027935`	Rab13	Ras-related protein Rab-13	2	2	1	12.5	Ref	AAA, AAA_16, AAA_22, AAA_25, AAA_33, AAA_7, ATP_bind_3, Arf, GTP_EFTU, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, RuvX, SRPRB, Septin, TMP-TENI, TniB
`ENSMUSG00000025142`	Aspscr1	Tether containing UBX domain for GLUT4	2	2	4	35.1	Ref	Cas9_C, QWRF, RBD, TUG-UBL1, UBX
`ENSMUSG00000001018`	Snapin	SNARE-associated protein Snapin	2	2	4	77.6	Ref	CATRA-C, DUF5798, E2F_TDP, Laminin_II, Snapin_Pallidin
`ENSMUSG00000002015`	Bcap31	B-cell receptor-associated protein 31	2	2	4	328.1	Novel	APG6_N, ATG14, Bap31, Bap31_Bap29_C, CENP-H, DUF3498, DUF4140, DUF4337, DUF4407, DUF724, DUF730, DUF948, ERM_C, GAS, KASH_CCD, LMBR1, LMF1, PIG-U, Rx_N, Sec34, TolA_bind_tri, YabA
`ENSMUSG00000021661`	Ankra2	Ankyrin repeat family A protein 2	2	2	1	20.0	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, Fer4_14, GIP1_N
`ENSMUSG00000019822`	Smpd2	Sphingomyelin phosphodiesterase 2	2	2	1	15.0	Ref	DUF2182, Exo_endo_phos
`ENSMUSG00000026566`	Mpzl1	Myelin protein zero-like protein 1	2	2	1	15.0	Ref	Adeno_E3_CR2, DUF4191, EphA2_TM, I-set, Ig_2, Ig_3, MDM31_MDM32, TraL, V-set, ig
`ENSMUSG00000027466`	Rbck1	RanBP-type and C3HC4-type zinc finger-containing protein 1	3	2	1	15.0	Novel	IBR, Rad50_zn_hook, ubiquitin, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-RanBP
`ENSMUSG00000031728`	Znf821	Zinc finger protein 821	2	2	1	7.5	Ref	CDC45, CPSF73-100_C, DUF5679, DUF5810, DUF6011, DZR, HMBD, Rad50_zn_hook, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_9, zf-C2H2_jaz, zf-Di19, zf-H2C2_2, zf-HYPF, zf-met
`ENSMUSG00000063445`	Nmral1	NmrA-like family domain-containing protein 1	2	2	1	45.1	Ref	3Beta_HSD, BRK, Epimerase, F420_oxidored, HTH_3, Helo_like_N, KR, NAD_binding_10, NmrA, Semialdhyde_dh, Shikimate_DH, TrkA_N, adh_short
`ENSMUSG00000001999`	Blvra	Biliverdin reductase A	2	2	1	127.7	Novel	Biliv-reduc_cat, GFO_IDH_MocA, HYPK_UBA, NAD_binding_3, SAM_KSR1
`ENSMUSG00000040276`	Pacsin1	Protein kinase C and casein kinase substrate in neurons protein 1	3	2	1	20.0	Ref	DUF2605, FCH, RPS31, SH3_1, SH3_2, SH3_9, Terpene_syn_C_2
`ENSMUSG00000030980`	Knop1	Lysine-rich nucleolar protein 1	2	2	1	5.0	Ref	DUF5771, SMAP
`ENSMUSG00000068039`	Tcp1	T-complex protein 1 subunit alpha	3	2	1	67.6	Ref	Cpn60_TCP1
`ENSMUSG00000020805`	Slc13a5	Na(+)/citrate cotransporter	2	2	1	2.5	Novel	CitMHS, DAGK_prokar, Na_sulph_symp
`ENSMUSG00000042787`	Exog	Nuclease EXOG, mitochondrial	3	2	1	10.0	Ref	DUF6730, Endonuclease_NS, Exog_C, YvrJ
`ENSMUSG00000037669`	Ldah	Lipid droplet-associated hydrolase	3	2	1	17.5	Ref	Abhydrolase_1, Abhydrolase_6, Hydrolase_4, LIDHydrolase, PaO, Ser_hydrolase
`ENSMUSG00000020458`	Rtn4	Reticulon-4	2	2	1	343.1	Ref	DNA_pol_phi, DUF4736, DUF639, NOA36, Phage_holin_3_6, Reticulon
`ENSMUSG00000028389`	Zfp37	Zinc finger protein 37	2	2	1	15.0	Ref	CpXC, DUF6434, GATA, HVO_2753_ZBP, HypA, KRAB, MBD, Nepo_coat, XPA_N, YhfH, Zn-ribbon_8, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HE, zf-H2C2_2, zf-LYAR, zf-met, zf_C2H2_13, zf_C2H2_ZHX, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000094936`	Rbm4b	RNA-binding protein 4B	2	2	1	22.5	Ref	Calcipressin, CompInhib_SCIN, DbpA, Nup35_RRM_2, RNA_bind, RRM_1, RRM_5, RRM_7, RRM_occluded, RSD-2, zf-CCHC, zf-CCHC_2, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000031389`	ARHGAP4	Rho GTPase-activating protein 4	2	2	1	2.5	Ref	CRIC_ras_sig, DDHD, DUF3439, FCH, RhoGAP, SH3_1, SH3_2, SH3_9, SKA2, SSP160
`ENSMUSG00000030086`	Chchd6	MICOS complex subunit Mic25	2	2	1	388.2	Ref	ATG14, Activator-TraM, CHCH, DUF1690, DUF3150, DUF4337, DUF6462, Exonuc_VII_L, FapA, IZUMO, MIC19_MIC25, NDUF_B7, hDGE_amylase
`ENSMUSG00000005699`	Pard6a	Partitioning defective 6 homolog alpha	5	2	1	72.6	Novel	PB1, PDZ, PDZ_6
`ENSMUSG00000025646`	Atrip	ATR-interacting protein	2	2	1	20.0	Ref	FapA, Fez1, Golgin_A5, HIP1_clath_bdg, HMMR_N, Lebercilin, Med21, OmpH, RNase_H_2, RNase_T, Tropomyosin_1
`ENSMUSG00000032423`	Syncrip	Heterogeneous nuclear ribonucleoprotein Q	2	2	1	25.0	Ref	Calcipressin, EBA-175_VI, MARF1_RRM1, PHM7_cyt, RRM_1, RRM_5, RRM_7, RRM_occluded
`ENSMUSG00000068040`	Tm9sf4	Transmembrane 9 superfamily member 4	2	2	1	2.5	Ref	DUF3169, DUF3817, DUF4229, EMP70
`ENSMUSG00000035228`	Ccdc106	Coiled-coil domain-containing protein 106	4	2	1	17.5	Novel	CCDC106, SOGA
`ENSMUSG00000028861`	Mrps15	Small ribosomal subunit protein uS15m	2	2	1	195.3	Novel	DUF272, DUF4140, DUF724, GPS2_interact, MIX23, PIN_4, Ribosomal_S15
`ENSMUSG00000031865`	Dctn1	Dynactin subunit 1	2	2	1	42.6	Novel	CAP_GLY, DUF2935, DUF4172, Dynactin, Fe_hyd_lg_C, Fez1, Filament
`ENSMUSG00000018858`	Mrpl58	Large ribosomal subunit protein mL62	2	2	1	52.6	Ref	HLH, NOG1_N, RF-1, pEK499_p136
`ENSMUSG00000099689`	ZNF383	Zinc finger protein 383	2	2	1	0.0	Novel	BHD_1, BolA, C1_4, DUF3741, DUF5830, DUF968, KRAB, Raffinose_syn, TFIIS_C, XPA_N, Zn-ribbon_8, Zn_Tnp_IS1595, Zn_ribbon_SprT, zf-BED, zf-C2H2, zf-C2H2_11, zf-C2H2_3rep, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HE, zf-H2C2_2, zf-met, zf-trcl, zf_C2H2_13, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000026857`	Ntmt1	N-terminal Xaa-Pro-Lys N-methyltransferase 1	2	2	1	72.6	Ref	Bin3, CTD4, Methyltransf_11, Methyltransf_12, Methyltransf_2, Methyltransf_23, Methyltransf_25, Methyltransf_29, Methyltransf_31, Methyltransf_PK, Ubie_methyltran
`ENSMUSG00000026179`	Pnkd	Probable thioesterase PNKD	3	2	1	215.4	Ref	HAGH_C, Lactamase_B, Lactamase_B_2, Lactamase_B_3
`ENSMUSG00000022961`	Son	Protein SON	3	2	1	37.6	Ref	RSRP
`ENSMUSG00000047261`	Gap43	Neuromodulin	3	2	1	375.6	Mixed	HEPN_AbiU2
`ENSMUSG00000030795`	Fus	RNA-binding protein FUS	2	2	1	20.0	Ref	KleE, RRM_1, zf-RanBP
`ENSMUSG00000033287`	KCTD17	BTB/POZ domain-containing protein KCTD17	2	2	1	52.6	Novel	BTB, BTB_2, Peptidase_M90, SAM_LFY
`ENSMUSG00000032112`	Trappc4	Trafficking protein particle complex subunit 4	4	2	1	182.8	Mixed	PDZ, PDZ_6, Sedlin_N, Sybindin
`ENSMUSG00000079557`	Marchf2	E3 ubiquitin-protein ligase MARCHF2	4	2	1	127.7	Mixed	Baculo_IE-1, RINGv, Vpu, zf-C3HC4, zf-RING_2, zf-rbx1
`ENSMUSG00000029723`	Tsc22d4	TSC22 domain family protein 4	2	2	1	17.5	Ref	DUF6262, DivIC, K-box, TSC22, YabA, ZapB, bZIP_2
`ENSMUSG00000027566`	Psma7	Proteasome subunit alpha type-7	2	2	1	701.2	Novel	DUF5069, Proteasome, Proteasome_A_N, gpW
`ENSMUSG00000029922`	Mkrn1	E3 ubiquitin-protein ligase makorin-1	2	2	1	12.5	Ref	B56, MKRN1_C, Prok-RING_4, Torus, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf-CCCH_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1, zf_CCCH_4
`ENSMUSG00000027270`	Lamp5	Lysosome-associated membrane glycoprotein 5	2	2	1	365.6	Ref	Chordopox_A13L, Cons_hypoth698, DUF4083, DUF819, Lamp, VAS1_LD
`ENSMUSG00000014856`	Tmem208	Transmembrane protein 208	4	2	1	150.3	Ref	DUF2207, DUF4191, Halogen_Hydrol, TMEM208_SND2
`ENSMUSG00000026171`	Rnf25	E3 ubiquitin-protein ligase RNF25	2	2	1	37.6	Ref	CpXC, DUF3785, Papo_T_antigen, RWD, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000067873`	Htatsf1	17S U2 SnRNP complex component HTATSF1	2	2	1	40.1	Ref	Nup35_RRM, RRM_1, RRM_7
`ENSMUSG00000003531`	Dgcr6	Protein DGCR6	3	2	1	117.7	Ref	DGCR6, DUF2458
`ENSMUSG00000001056`	Nhp2	H/ACA ribonucleoprotein complex subunit 2	2	2	1	67.6	Ref	ETC_C1_NDUFA5, Phage_RpbA, Ribosomal_L31e, Ribosomal_L7Ae, UPF0203, zinc_ribbon_12
`ENSMUSG00000020083`	Fam241b	Protein FAM241B	3	2	1	57.6	Ref	DUF4605
`ENSMUSG00000001211`	Agpat3	1-acyl-sn-glycerol-3-phosphate acyltransferase gamma	3	2	1	45.1	Ref	Acyltransf_C, Acyltransferase
`ENSMUSG00000027642`	Rpn2	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2	3	2	1	37.6	Novel	DUF5859, HSM3_N, PI3K_rbd, Ribophorin_II
`ENSMUSG00000036564`	ndrg4	Protein NDRG4	4	2	1	102.7	Mixed	Abhydrolase_1, Endotoxin_N, Ndr
`ENSMUSG00000107068`	Pagr1a	PAXIP1-associated glutamate-rich protein 1A	2	2	1	110.2	Novel	CD225, Med13_C, Neisseria_TspB, Nop53, PAXIP1_C
`ENSMUSG00000032046`	Abhd12	Lysophosphatidylserine lipase ABHD12	2	2	1	172.8	Ref	Abhydrolase_1, Abhydrolase_11, Abhydrolase_3, Abhydrolase_6, Hydrolase_4, Peptidase_S15, RAP, Say1_Mug180, Thioesterase
`ENSMUSG00000050711`	Scg2	Secretogranin-2	2	2	1	45.1	Ref	Granin, IF2_N, eIF3_N
`ENSMUSG00000020818`	Mfsd11	UNC93-like protein MFSD11	2	2	1	7.5	Ref	DUF6498, MFS_1, UNC-93
`ENSMUSG00000000861`	Bcl11a	BCL11 transcription factor A	2	2	1	42.6	Ref	Astro_capsid_p, BSP_II, C1_4, DDHD, DNA_pol_phi, DUF2256, DUF3268, DUF4820, FAM32A, FYVE, Importin_rep_6, NOA36, Nop14, PPP4R2, RNA_pol_3_Rpc31, Ribosomal_60s, TUTase, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-H2C2_2, zf-H2C2_5, zf-HIT, zf_UBZ
`ENSMUSG00000033099`	Nol12	Nucleolar protein 12	2	2	1	15.0	Novel	ALC, FKBP_N, Nop25, Nup54_C
`ENSMUSG00000027593`	Raly	RNA-binding protein Raly	5	2	1	115.2	Mixed	Filament_head, GRP, Herpes_capsid, RRM_1, RRM_5, RhlB
`ENSMUSG00000016833`	Mrps18c	Small ribosomal subunit protein bS18m	2	2	1	125.2	Ref	CATRA-N, Cript, Fer4_11, Ribosomal_S18
`ENSMUSG00000023021`	Cers5	Ceramide synthase 5	2	2	1	12.5	Ref	DUF1003, Homeodomain, TRAM_LAG1_CLN8
`ENSMUSG00000071644`	Eef1g	Elongation factor 1-gamma	2	2	1	62.6	Ref	EF1G, GST_C, GST_C_2, GST_C_3, GST_N, GST_N_2, GST_N_3
`ENSMUSG00000008305`	Tle1	Transducin-like enhancer protein 1	2	2	1	5.0	Ref	ANAPC4_WD40, Cytochrom_D1, HALZ, NBCH_WD40, Nup160, Ribosomal_S18, Spc24, TLE_N, Transketolase_N, WD40, WD40_like
`ENSMUSG00000061086`	Myl4	Myosin light chain 4	3	2	1	72.6	Novel	EF-hand_1, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9
`ENSMUSG00000078941`	Ak6	Adenylate kinase isoenzyme 6	3	2	1	157.8	Mixed	AAA, AAA_17, AAA_18, AAA_22, AAA_28, AAA_33, ADK, Cytidylate_kin, Cytidylate_kin2, RNA_helicase, SKI, dNK
`ENSMUSG00000027706`	Sec62	Translocation protein SEC62	2	2	1	20.0	Novel	BUD22, CIS_TMP, DEP, Dsh_C, EOS1, RR_TM4-6, SLC12, Sec62, YhhN
`ENSMUSG00000032673`	Prorsd1	Prolyl-tRNA synthetase associated domain-containing protein 1	2	2	1	12.5	Ref	DpnI_C, PRORP, SinI, tRNA_edit
`ENSMUSG00000055762`	Eef1d	Elongation factor 1-delta	6	2	1	47.6	Mixed	CCD48, EF-1_beta_acid, EF1_GNE, JIP_LZII, KASH_CCD, Lzipper-MIP1, PRKG1_interact, PilJ, RF-1, Striatin, TSC22, TolA_bind_tri, Trimer_CC
`ENSMUSG00000034321`	Exosc1	Exosome complex component CSL4	2	2	1	35.1	Novel	ECR1_N, EXOSC1
`ENSMUSG00000055850`	Rnf181	E3 ubiquitin-protein ligase RNF181	2	2	1	125.2	Novel	BIR, FANCL_C, PHD, Prok-RING_1, Prok-RING_4, RINGv, Rad50_zn_hook, UBZ_FAAP20, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000038244`	Mical2	[F-actin]-monooxygenase MICAL2	3	2	1	95.2	Mixed	AlaDh_PNT_C, CAMSAP_CH, CH, DAO, FAD_binding_2, FAD_binding_3, FAD_oxidored, GIDA, HI0933_like, LIM, Lycopene_cycl, NAD_binding_7, NAD_binding_8, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Thi4
`ENSMUSG00000078584`	—	Uncharacterized protein C1orf50 homolog	2	2	1	7.5	Ref	DUF2452, DUF3975, DUF5993, Gallidermin
`ENSMUSG00000071655`	Ubxn1	UBX domain-containing protein 1	3	2	1	245.4	Ref	CDC45, Presenilin, Selenoprotein_S, UBA, UBX, eIF3_subunit
`ENSMUSG00000026203`	Dnajb2	DnaJ homolog subfamily B member 2	2	2	1	42.6	Ref	DnaJ, RPT, UIM
`ENSMUSG00000050471`	Fam118b	Protein FAM118B	2	2	1	0.0	Ref	DUF5443, SIR2_2
`ENSMUSG00000029131`	Dnajb6	DnaJ homolog subfamily B member 6	2	2	1	388.2	Ref	DnaJ, eIF3_subunit
`ENSMUSG00000053801`	Grwd1	Glutamate-rich WD repeat-containing protein 1	2	2	1	15.0	Ref	ANAPC4_WD40, CAF1C_H4-bd, CENP-B_dimeris, IKI3, Voldacs, WD40
`ENSMUSG00000026223`	Itm2c	Integral membrane protein 2C	2	2	1	1,252.2	Novel	BRICHOS, DAG_kinase_N
`ENSMUSG00000050891`	Tatdn1	Deoxyribonuclease TATDN1	2	2	1	180.3	Ref	MGAT2, TatD_DNase, ToxB_N
`ENSMUSG00000026860`	Sh3glb2	Endophilin-B2	4	2	1	132.7	Ref	BAR, BAR_2, BAR_3, IMD, SH3_1, SH3_2, SH3_9
`ENSMUSG00000036932`	Aifm1	Apoptosis-inducing factor 1, mitochondrial	2	2	1	12.5	Ref	AIF-MLS, AIF_C, Amino_oxidase, DUF3918, FAD_oxidored, HI0933_like, Lys_Orn_oxgnase, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Reductase_C, TPP_enzyme_M, Trp_halogenase
`ENSMUSG00000024587`	Nars1	Asparagine--tRNA ligase, cytoplasmic	2	2	1	95.2	Ref	Cir_N, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon, tRNA_anti-like, tRNA_anti_2
`ENSMUSG00000017843`	Ppp2r5c	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform	2	2	1	47.6	Ref	B56, Sec7_N, T4_Rnl2_C
`ENSMUSG00000040746`	Rnf167	E3 ubiquitin-protein ligase RNF167	2	2	1	100.2	Novel	CTU2, FAM176, FANCL_C, PA, PHD, Prok-RING_1, Prok-RING_4, RINGv, RecR, zf-ANAPC11, zf-C3H2C3, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING-like, zf-RING_11, zf-RING_15, zf-RING_16, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-UDP, zf-rbx1
`ENSMUSG00000019194`	Scn1b	Sodium channel regulatory subunit beta-1	2	2	1	215.4	Novel	DUF6264, Ig_2, Ig_3, Protocadherin, V-set, ig
`ENSMUSG00000037364`	Srrt	Serrate RNA effector molecule homolog	2	2	1	17.5	Ref	ARS2, DUF4187, DUF5923, FAM184, Hid1, RRM_1, RR_TM4-6, SAPS, SERRATE_Ars2_N, SLC12, Zip
`ENSMUSG00000020849`	YWHAE	14-3-3 protein epsilon	2	2	1	192.8	Novel	14-3-3, Orbi_VP5, Ta0938
`ENSMUSG00000068739`	Sars1	Serine--tRNA ligase, cytoplasmic	2	2	1	75.1	Ref	NOD, PG_binding_2, Seryl_tRNA_N, VOMI, tRNA-synt_2b
`ENSMUSG00000018809`	Smyd4	Protein-lysine N-methyltransferase SMYD4	2	2	1	12.5	Novel	ANAPC3, FH2, MscS_porin, PEX11, SET, TPR_12, TPR_14, TPR_19, TPR_2, TPR_20, zf-MYND
`ENSMUSG00000036503`	Rnf13	E3 ubiquitin-protein ligase RNF13	2	2	1	17.5	Ref	Baculo_11_kDa, CTU2, DNA_ligase_ZBD, FeoB_associated, HTH_63, LapA_dom, Neur_chan_memb, Ninjurin, PA, PHD, Prok-RING_4, RINGv, SID-1_RNA_chan, zf-ANAPC11, zf-C3H2C3, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000035863`	Palm	Paralemmin-1	2	2	1	57.6	Ref	FeoB_Cyto, Paralemmin
`ENSMUSG00000014232`	Cluap1	Clusterin-associated protein 1	2	2	1	25.0	Ref	ATG14, BUD22, CENP-B_dimeris, Cluap1, Jnk-SapK_ap_N, Ndc80_HEC, RasGAP_C, Tweety
`ENSMUSG00000046721`	Rpl14	Large ribosomal subunit protein eL14	2	2	1	1,813.1	Novel	KOW, Ribosomal_L14e, UPF0688
`ENSMUSG00000024797`	Vps51	Vacuolar protein sorting-associated protein 51 homolog	2	2	1	10.0	Ref	COG2, COG5, DUF3829, Dor1, GIT_CC, Occludin_ELL, Sec5, TMTC_DUF1736, Vps51, Vps53_N, Vps54_N, Zw10
`ENSMUSG00000006095`	Tbcb	Tubulin-folding cofactor B	2	2	1	408.2	Ref	CAP_GLY, RSV_NS2, Ubiquitin_2
`ENSMUSG00000039217`	Il18	Interleukin-18	2	2	1	7.5	Novel	DUF273, IL1, NCA2
`ENSMUSG00000013593`	Ndufs2	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial	2	2	1	237.9	Novel	Complex1_49kDa, NiFeSe_Hases
`ENSMUSG00000025290`	RPS24	Small ribosomal subunit protein eS24	4	2	1	1,795.6	Ref	Ribosomal_S24e, YrbL-PhoP_reg
`ENSMUSG00000044811`	Cd300c2	CMRF-35-like molecule 4	2	2	1	15.0	Ref	Apq12, Ig_3, V-set, ig
`ENSMUSG00000033760`	Rbm4b	RNA-binding protein 4B	3	2	1	10.0	Ref	Calcipressin, DbpA, RNA_bind, RRM_1, RRM_5, RRM_7, RRM_occluded, zf-CCHC, zf-CCHC_2, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000059436`	Max	Protein max	2	2	1	45.1	Ref	ATG16, Csm1_N, DUF4140, DUF4763, DUF5595, DUF5716, DivIC, FapA, Filament, HALZ, HLH, JAKMIP_CC3, Jnk-SapK_ap_N, SLX9, SlyX, V_ATPase_I, ZapB, bZIP_2
`ENSMUSG00000053768`	Chchd3	MICOS complex subunit Mic19	2	2	1	75.1	Novel	CHCH, DUF1690, DUF885, ECH_2, Fes1, MIC19_MIC25, NDUF_B7
`ENSMUSG00000037852`	Cpe	Carboxypeptidase E	2	2	1	1,362.4	Novel	AstE_AspA, CarbopepD_reg_2, CarboxypepD_reg, PEGA, Peptidase_M14, Phage_tube_C
`ENSMUSG00000004268`	Emg1	Ribosomal RNA small subunit methyltransferase NEP1	2	2	1	147.8	Novel	CHMI, EMG1
`ENSMUSG00000038876`	Echdc1	Ethylmalonyl-CoA decarboxylase	3	2	1	65.1	Ref	ECH_1, ECH_2, Prok-RING_4, Sp-DndD, WWE, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000030638`	Sh3gl3	Endophilin-A3	2	2	1	7.5	Ref	BAR, BAR_2, BAR_3, PPP5, SH3_1, SH3_2, SH3_9
`ENSMUSG00000002504`	Nherf2	Na(+)/H(+) exchange regulatory cofactor NHE-RF2	3	2	1	27.5	Ref	DUF5994, EBP50_C, GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000021792`	Prxl2a	Peroxiredoxin-like 2A	3	2	1	127.7	Novel	AhpC-TSA, AhpC-TSA_2, DUF2231, Redoxin, UCR_Fe-S_N
`ENSMUSG00000034563`	Ccpg1	Cell cycle progression protein 1	2	2	1	27.5	Ref	Csm1_N, DHR-2_Lobe_C, DUF1664, DUF948, YtxH
`ENSMUSG00000052384`	Nrros	Transforming growth factor beta activator LRRC33	2	2	1	7.5	Ref	LRRNT, LRR_1, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9
`ENSMUSG00000019978`	Epb41l2	Band 4.1-like protein 2	2	2	1	5.0	Ref	4_1_CTD, FA, FERM_C, FERM_M, FERM_N, Gly_transf_sug, SAB
`ENSMUSG00000001833`	Septin7	Septin-7	2	2	1	15.0	Novel	AAA_16, AAA_22, AAA_29, ABC_tran, AIG1, ChrB_N, DUF6683, Exonuc_VII_L, FeoB_N, Fez1, Gtr1_RagA, LMBR1, MMR_HSR1, Ras, Roc, RsgA_GTPase, Septin
`ENSMUSG00000037196`	Pacrg	Parkin coregulated gene protein homolog	2	2	1	2.5	Novel	DCB, ParcG, Peptidase_C107, RNA_pol_Rpb2_2
`ENSMUSG00000002289`	Angptl4	Angiopoietin-related protein 4	2	2	1	20.0	Ref	COG5, DUF16, DUF5798, DivIC, Fib_alpha, Fibrinogen_C, Helo_like_N, HrpB7, HrpJ, SlyX, UPF0242, XhlA
`ENSMUSG00000021546`	HNRNPK	Heterogeneous nuclear ribonucleoprotein K	4	2	1	105.2	Ref	DJ-1_PfpI, DUF719, KH_1, KH_2, KH_4, KH_5, MOEP19, ROKNT
`ENSMUSG00000028657`	Ppt1	Palmitoyl-protein thioesterase 1	3	2	1	22.5	Mixed	Palm_thioest
`ENSMUSG00000026921`	Egfl7	Epidermal growth factor-like protein 7	3	2	1	15.0	Mixed	EGF, EGF_2, EGF_CA, EMI
`ENSMUSG00000005873`	Reep5	Receptor expression-enhancing protein 5	2	2	1	217.9	Novel	Phage_holin_3_6, TB2_DP1_HVA22
`ENSMUSG00000031068`	Glrx3	Glutaredoxin-3	2	2	1	20.0	Novel	Arf, CNOT1_HEAT_N, DIM1, DUF4279, DUF953, GREB1_C, GST_N_3, GerD, Glutaredoxin, HeH, HyaE, OST3_OST6, Phosducin, RdRP, SH3BGR, TMF_TATA_bd, Thioredoxin, Thioredoxin_2, Thioredoxin_8, Thioredoxin_9, TrbC_Ftype, ZinT
`ENSMUSG00000030824`	Nucb1	Nucleobindin-1	2	2	1	22.5	Ref	EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, RNase_Y_N
`ENSMUSG00000056116`	H2-Q10	H-2 class I histocompatibility antigen, Q10 alpha chain	2	2	1	17.5	Ref	AA_permease_2, C1-set, C2-set_2, Cadherin_C_2, Ig_3, MHC_I, Mucin, ig
`ENSMUSG00000030226`	LMO3	LIM domain only protein 3	2	2	1	47.6	Ref	LIM, Siva
`ENSMUSG00000038005`	Hpf1	Histone PARylation factor 1	4	2	1	135.2	Mixed	DUF2228, DUF6027
`ENSMUSG00000063457`	RPS15	Small ribosomal subunit protein uS19	2	2	1	2,091.1	Ref	LCAT, MAP65_ASE1, Prok_Ub, Ribosomal_S19
`ENSMUSG00000028063`	Lmna	Prelamin-A/C	2	2	1	67.6	Ref	ATG16, CENP-F_leu_zip, Crescentin, DUF4407, FIVAR, Fez1, Filament, GBR2_CC, HAUS-augmin3, LTD, TPR_MLP1_2, Tht1, TolA_bind_tri, zf-NADH-PPase
`ENSMUSG00000073131`	Vma21	Vacuolar ATPase assembly integral membrane protein Vma21	2	2	1	0.0	Ref	DUF6773, ExoD, FA_desaturase, SLATT_3, VMA21
`ENSMUSG00000061032`	Rrp1	Ribosomal RNA processing protein 1 homolog A	2	2	1	370.6	Ref	DNA_pol_phi, DRIM, DUF1981, DUF4391, DUF4776, MMS19_N, Nop52, PFam54_60, Tn7_Tnp_TnsA_C
`ENSMUSG00000026333`	Gin1	Gypsy retrotransposon integrase-like protein 1	2	2	1	7.5	Ref	Cytochrom_D1, Integrase_H2C2, rve, zf-H2C2
`ENSMUSG00000037788`	Vopp1	WW domain binding protein VOPP1	2	2	1	55.1	Ref	Conotoxin_I2, FixQ, TMEM52, WBP-1, zf-His_Me_endon
`ENSMUSG00000022559`	Fbxl6	F-box/LRR-repeat protein 6	3	2	1	20.0	Ref	LRR_4, LRR_6, LRR_8
`ENSMUSG00000025878`	Uimc1	BRCA1-A complex subunit RAP80	2	2	1	5.0	Ref	DUF1645, LIDHydrolase, RAP80_UIM, Rad50_zn_hook, UBZ_FAAP20, UIM, XAF1_C, zf_UBZ
`ENSMUSG00000029610`	Aimp2	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2	2	2	1	97.7	Ref	AIMP2_LysRS_bd, GST_C, GST_C_2, GST_C_3, GST_C_6, GTP-bdg_M, Thioredoxin_16
`ENSMUSG00000037032`	Apbb1	Amyloid beta precursor protein binding family B member 1	5	2	1	285.5	Mixed	PID, PID_2, PTB, WW
`ENSMUSG00000022684`	Bfar	Bifunctional apoptosis regulator	2	2	1	7.5	Novel	Baculo_IE-1, DUF2076, Prok-RING_4, SAM_1, SAM_2, SAM_PNT, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000075704`	Txnrd2	Thioredoxin reductase 2, mitochondrial	2	2	1	37.6	Ref	AlaDh_PNT_C, DAO, FAD_binding_2, FAD_oxidored, GIDA, HI0933_like, Lycopene_cycl, Lys_Orn_oxgnase, NAD_binding_8, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Pyr_redox_dim, Thi4
`ENSMUSG00000024369`	Nelfe	Negative elongation factor E	3	2	1	17.5	Ref	Nup35_RRM_2, RPA43_OB, RRM_1, RRM_occluded
`ENSMUSG00000029472`	Anapc5	Anaphase-promoting complex subunit 5	3	2	1	42.6	Ref	ANAPC5, MapZ_EC1, TPR_1, TPR_10, TPR_12, TPR_14, TPR_19, TPR_2, TPR_4, TPR_7, VP4_haemagglut, YycI
`ENSMUSG00000066037`	HNRNPR	Heterogeneous nuclear ribonucleoprotein R	3	2	1	27.5	Ref	Nup35_RRM_2, RRM_1, RRM_5, RRM_7, RRM_occluded, Spo7_2_N, hnRNP_Q_AcD
`ENSMUSG00000041199`	Rpusd1	RNA pseudouridylate synthase domain-containing protein 1	4	2	1	92.7	Ref	PseudoU_synth_2
`ENSMUSG00000028795`	Ccdc28b	Coiled-coil domain-containing protein 28B	2	2	1	85.1	Ref	CCDC28, DUF892
`ENSMUSG00000035824`	Tk2	Thymidine kinase 2, mitochondrial	2	2	1	15.0	Ref	AAA_18, AAA_33, CoaE, RE_Bpu10I, Thymidylate_kin, dNK
`ENSMUSG00000029713`	GNB2	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	2	2	1	273.0	Ref	ANAPC4_WD40, CaM_bind, Cytochrom_D1, Ge1_WD40, NBCH_WD40, WD40, WD40_like
`ENSMUSG00000029048`	Rer1	Protein RER1	2	2	1	65.1	Ref	DUF2208, Rer1, SEP
`ENSMUSG00000028447`	Dctn3	Dynactin subunit 3	2	2	1	50.1	Novel	DUF4011, DivIC, Dynactin_p22, Pox_A_type_inc, Spidroin_MaSp, TetR_C_24
`ENSMUSG00000049600`	Zbtb45	Zinc finger and BTB domain-containing protein 45	2	2	1	25.0	Novel	BTB, DUF2256, HypA, LIM, Zn-ribbon_8, Zn_Tnp_IS1595, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HC_2, zf-Di19, zf-H2C2_2, zf-UBP, zf-met, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000025858`	Get4	Golgi to ER traffic protein 4 homolog	2	2	1	47.6	Ref	GET4, SDH_C, Vps39_1
`ENSMUSG00000028180`	ZRANB2	Zinc finger Ran-binding domain-containing protein 2	2	2	1	15.0	Ref	DUF35_N, zf-RanBP
`ENSMUSG00000028266`	LMO4	LIM domain transcription factor LMO4	3	2	1	172.8	Novel	Arc_trans_TRASH, DUF2175, DUF6510, DUF678, LIM, zf-dskA_traR, zf_UBZ
`ENSMUSG00000024853`	Sf3b2	Splicing factor 3B subunit 2	2	2	1	97.7	Ref	DUF382, PSP, SAP, SAP_new25
`ENSMUSG00000030008`	Pradc1	Protease-associated domain-containing protein 1	2	2	1	10.0	Novel	PA, PRIMA1
`ENSMUSG00000038708`	Golga4	Golgin subfamily A member 4	2	2	1	15.0	Ref	DUF3800, GRIP
`ENSMUSG00000033728`	Lrrc14	Leucine-rich repeat-containing protein 14	2	2	1	5.0	Ref	Fusion_gly, Gram_pos_anchor, I-set, IPTL-CTERM, Ig_2, Ig_3, LRR_1, LRR_4, LRR_6, LRR_8, MmeI_TRD, RIIa
`ENSMUSG00000037089`	Slc35b2	Adenosine 3'-phospho 5'-phosphosulfate transporter 1	2	2	1	35.1	Ref	DUF2070, EamA, PI3K_rbd, TPT, UAA
`ENSMUSG00000061111`	Mcrip1	Mapk-regulated corepressor-interacting protein 1	2	2	1	227.9	Ref	DUF6551, FAM195, LAP2alpha
`ENSMUSG00000057113`	Npm1	Nucleophosmin	3	2	1	70.1	Ref	CDC45, NOA36, NPM1-C, Nop14, Nucleoplasmin, RRN3, SDA1, TFIIF_alpha
`ENSMUSG00000035949`	Fbxw2	F-box/WD repeat-containing protein 2	3	2	1	52.6	Ref	ANAPC4_WD40, DUF5570, F-box, F-box-like, NBCH_WD40, WD40
`ENSMUSG00000039323`	Igfbp2	Insulin-like growth factor-binding protein 2	2	2	1	55.1	Ref	Cys_rich_CWC, IGFBP, Thyroglobulin_1
`ENSMUSG00000050043`	Tmx2	Thioredoxin-related transmembrane protein 2	2	2	1	140.2	Ref	PAP2_3, Thioredoxin
`ENSMUSG00000001844`	Zdhhc4	Palmitoyltransferase ZDHHC4	2	2	1	5.0	Novel	DHHC, DUF417
`ENSMUSG00000060780`	Lrrtm1	Leucine-rich repeat transmembrane neuronal protein 1	3	2	1	12.5	Novel	HemY_N, LRRNT, LRR_1, LRR_12, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9, Ni_hydr_CYTB, UL97
`ENSMUSG00000031516`	Dctn6	Dynactin subunit 6	3	2	1	300.5	Novel	Hexapep
`ENSMUSG00000048495`	Tyw5	tRNA wybutosine-synthesizing protein 5	2	2	1	47.6	Ref	Cupin_8, Hpr_kinase_N, JmjC, JmjC_2
`ENSMUSG00000075467`	Dnlz	DNL-type zinc finger protein	2	2	1	7.5	Ref	DUF6112, DUF6510, HypA, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-CSL, zf-DNL
`ENSMUSG00000024957`	Kcnk4	Potassium channel subfamily K member 4	3	2	1	15.0	Ref	G3P_acyltransf, IRK, Ion_trans, Ion_trans_2
`ENSMUSG00000024026`	Glo1	Lactoylglutathione lyase	2	2	1	105.2	Ref	Glyoxalase, Glyoxalase_4, Glyoxalase_6, PIG-X
`ENSMUSG00000029199`	Lias	Lipoyl synthase, mitochondrial	2	2	1	27.5	Ref	Glyco_transf_4, LIAS_N, ROS_MUCR, Radical_SAM, SVWC
`ENSMUSG00000048277`	Syngr2	Synaptogyrin-2	2	2	1	25.0	Ref	CD20, DUF1761, MARVEL
`ENSMUSG00000036613`	Eipr1	EARP and GARP complex-interacting protein 1	2	2	1	52.6	Ref	Gly-rich_Ago1, WD40
`ENSMUSG00000078451`	Ppil6	Probable inactive peptidyl-prolyl cis-trans isomerase-like 6	2	2	1	5.0	Ref	CysA_C_terminal, DUF5309, Pro_isomerase
`ENSMUSG00000057469`	E2f6	Transcription factor E2F6	2	2	1	2.5	Ref	BRCA-2_OB3, DUF5719, DUF5908, DUF603, E2F_CC-MB, E2F_TDP, TrmB, vATP-synt_E
`ENSMUSG00000022634`	Yaf2	YY1-associated factor 2	2	2	1	52.6	Ref	C1_2, Ephrin_rec_like, PROCN, RAP1, WTX, YAF2_RYBP, zf-RanBP
`ENSMUSG00000062822`	—	Uncharacterized protein C5orf34 homolog	2	2	1	5.0	Ref	DUF4520, DUF4524, Lipocalin
`ENSMUSG00000030750`	Nsmce1	Non-structural maintenance of chromosomes element 1 homolog	2	2	1	75.1	Novel	C1_1, DZR_2, SMC_Nse1, TMEMspv1-c74-12, zf-C3HC4, zf-C3HC4_2, zf-RING-like
`ENSMUSG00000020792`	Exoc7	Exocyst complex component 7	3	2	1	5.0	Ref	COG5, Exo70, RolB_RolC, Vps52
`ENSMUSG00000020912`	Krt12	Keratin, type I cytoskeletal 12	2	2	1	70.1	Novel	ADIP, APC_N_CC, ATG16, Bacillus_HBL, COG2, DUF1664, DUF4407, DivIC, ERM_helical, Filament, LPP, Mod_r, SKA1, Sec2p, Sec34, Tweety, Uso1_p115_C, V_ATPase_I, YabA
`ENSMUSG00000018398`	Septin8	Septin-8	3	2	1	150.3	Ref	AAA_16, AAA_22, AAA_23, AAA_24, AAA_29, AAA_7, ABC_tran, AIG1, ATG14, ATP_bind_1, DUF5906, DUF87, Dynamin_N, GTP_EFTU, IMD, MMR_HSR1, RHD3_GTPase, RNA_helicase, Ras, Roc, RsgA_GTPase, Septin, T2SSE
`ENSMUSG00000019970`	Sgk1	Serine/threonine-protein kinase Sgk1	3	2	1	75.1	Ref	ABC1, APH, FTA2, Kdo, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_C
`ENSMUSG00000008668`	RPS18	Small ribosomal subunit protein uS13	2	2	1	205.4	Ref	H2TH, NFACT_N, PGM_PMM_II, PP28, Ribosomal_S13
`ENSMUSG00000078695`	Cisd3	CDGSH iron-sulfur domain-containing protein 3, mitochondrial	3	2	1	147.8	Ref	Inhibitor_I71, Toxin_14, zf-CDGSH
`ENSMUSG00000002625`	Akap8l	A-kinase anchor protein 8-like	2	2	1	50.1	Ref	AKAP95, CDC45, SURF2, zf-C2H2_2
`ENSMUSG00000018572`	Phf23	PHD finger protein 23	2	2	1	10.0	Ref	C1_1, EBV-NA3, Nha1_C, PHD, PHD_2, PHD_4, Pox_Ag35, RNA_pol_3_Rpc31, Ribosomal_60s, zf-HC5HC2H, zf-RING_9
`ENSMUSG00000024949`	Sf1	Splicing factor 1	3	2	1	32.6	Ref	KH_1, KH_5, SF1-HH, zf-CCHC, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000029126`	Nsg1	Neuronal vesicle trafficking-associated protein 1	3	2	1	92.7	Mixed	Calcyon, DUF1581, DUF2448, DUF535
`ENSMUSG00000034681`	RNPS1	RNA-binding protein with serine-rich domain 1	3	2	1	42.6	Ref	Macoilin, Nup35_RRM_2, PI3K_1B_p101, RRM_1, RRM_3, RRM_5, RRM_7, RRM_occluded
`ENSMUSG00000021996`	Esd	S-formylglutathione hydrolase	2	2	1	77.6	Ref	AXE1, Abhydrolase_2, Abhydrolase_3, BD-FAE, Chlorophyllase, Chlorophyllase2, Esterase, Esterase_PHB, Hydrolase_4, Lipase_3, Peptidase_S9, Shadoo
`ENSMUSG00000026096`	Osgepl1	tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial	2	2	1	7.5	Ref	BcrAD_BadFG, CheC, DUF3659, NADH_4Fe-4S, TsaD
`ENSMUSG00000068329`	Htra2	Serine protease HTRA2, mitochondrial	2	2	1	47.6	Ref	GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Peptidase_S32, Peptidase_S46, Trypsin, Trypsin_2
`ENSMUSG00000039903`	Eva1c	Protein eva-1 homolog C	2	2	1	0.0	Ref	FAM176, Gal_Lectin, Tnp_22_trimer
`ENSMUSG00000041112`	Elmo1	Engulfment and cell motility protein 1	2	2	1	112.7	Ref	ELMO_ARM, ELMO_CED12, FERM_N, Imm42, Nup96, PH_12, ZFYVE21_C
`ENSMUSG00000040667`	Nup88	Nuclear pore complex protein Nup88	2	2	1	32.6	Ref	BLOC1_2, DUF4208, DUF4404, Exonuc_VII_L, FliJ, Importin_rep_6, Latarcin, Nup88
`ENSMUSG00000037740`	Mrps26	Small ribosomal subunit protein mS26	2	2	1	107.7	Ref	DUF1127, MAJIN, MRP-S26, PBP_sp32
`ENSMUSG00000024181`	Mrpl28	Large ribosomal subunit protein bL28m	3	2	1	285.5	Ref	Ribosomal_L28
`ENSMUSG00000026245`	Farsb	Phenylalanine--tRNA ligase beta subunit	4	2	1	47.6	Mixed	B3_4, B5, EAD7, PhetRS_B1, tRNA_synthFbeta
`ENSMUSG00000031862`	Atp13a1	Endoplasmic reticulum transmembrane helix translocase	2	2	1	10.0	Ref	Cation_ATPase, DHHC, E1-E2_ATPase, Hydrolase, Hydrolase_3
`ENSMUSG00000026701`	Prdx6	Peroxiredoxin-6	3	2	1	450.8	Ref	1-cysPrx_C, AhpC-TSA, Redoxin
`ENSMUSG00000000326`	Comt	Catechol O-methyltransferase	2	2	1	100.2	Novel	CmcI, Methyltransf_24, Methyltransf_3, Nitroreductase
`ENSMUSG00000057554`	Lgals8	Galectin-8	3	2	1	12.5	Ref	Gal-bind_lectin
`ENSMUSG00000024539`	Ptpn2	Tyrosine-protein phosphatase non-receptor type 2	2	2	1	20.0	Ref	CHASE3, DSPc, RIC3, Y_phosphatase, Y_phosphatase3
`ENSMUSG00000029319`	Coq2	4-hydroxybenzoate polyprenyltransferase, mitochondrial	2	2	1	45.1	Novel	DUF2798, UbiA
`ENSMUSG00000031948`	Kars1	Lysine--tRNA ligase	3	2	1	32.6	Ref	DUF4407, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon
`ENSMUSG00000027805`	PFN2	Profilin-2	2	2	1	470.8	Ref	DUF2076, DUF4106, Profilin
`ENSMUSG00000053453`	Thoc7	THO complex subunit 7 homolog	2	2	1	152.8	Novel	AATF-Che1, COG2, Com_YlbF, DASH_Dad2, DUF1664, DUF4407, EcoEI_R_C, FapA, Med9, THOC7, Tup_N, UPF0193, V_ATPase_I, ubiquitin
`ENSMUSG00000031371`	Haus7	HAUS augmin-like complex subunit 7	2	2	1	27.5	Ref	AATF-Che1, FlgN, Nsp1_C, Plant_NMP1, YabA
`ENSMUSG00000034757`	Tmub2	Transmembrane and ubiquitin-like domain-containing protein 2	3	2	1	72.6	Ref	CD52, DUF6232, Dsc3_N, Rad60-SLD_2, ubiquitin
`ENSMUSG00000038880`	Mrps34	Small ribosomal subunit protein mS34	2	2	1	182.8	Ref	MRP-S34, NDK, PSI_PsaJ, Peptidase_S77
`ENSMUSG00000020024`	Cep83	Centrosomal protein of 83 kDa	2	2	1	10.0	Ref	FtsL_2, HTH_53
`ENSMUSG00000032186`	Tmod2	Tropomodulin-2	4	2	1	12.5	Ref	LRR_6, Peptidase_M3_N, Tropomodulin
`ENSMUSG00000037742`	EEF1A1	Elongation factor 1-alpha 1	2	2	1	3,976.9	Novel	Dynamin_N, G-alpha, GTP_EFTU, GTP_EFTU_D2, GTP_EFTU_D3, GTP_EFTU_D4, Gtr1_RagA, MMR_HSR1
`ENSMUSG00000062006`	Rpl34	Large ribosomal subunit protein eL34	3	2	1	107.7	Ref	Ribosomal_L34e
`ENSMUSG00000057236`	RBBP4	Histone-binding protein RBBP4	5	2	1	60.1	Mixed	ANAPC4_WD40, CAF1C_H4-bd, Capsid_NCLDV, NBCH_WD40, WD40, eIF2A
`ENSMUSG00000027953`	Slc50a1	Sugar transporter SWEET1	3	2	1	57.6	Mixed	MtN3_slv, PQ-loop, YhhN
`ENSMUSG00000006392`	Med8	Mediator of RNA polymerase II transcription subunit 8	2	2	1	72.6	Ref	Baculo_PEP_C, CENP-Q, CSN4_RPN5_eIF3a, Med8, SlyX
`ENSMUSG00000007950`	Abhd8	Protein ABHD8	2	2	1	255.4	Novel	Abhydrolase_1, Abhydrolase_2, Abhydrolase_4, Abhydrolase_6, Abhydrolase_8, BAAT_C, BD-FAE, Chlorophyllase2, DLH, DUF2920, DUF4663, Esterase, FSH1, Hydrolase_4, LIP, Palm_thioest, Peptidase_S15, Peptidase_S9, Ser_hydrolase, Trypan_PARP
`ENSMUSG00000021087`	Rtn1	Reticulon-1	3	2	1	1,487.6	Mixed	Reticulon
`ENSMUSG00000029486`	Mrpl1	Large ribosomal subunit protein uL1m	2	2	1	10.0	Ref	Ribosomal_L1, Wound_ind
`ENSMUSG00000047084`	Ngrn	Neugrin	2	2	1	92.7	Ref	Dermcidin, MRP-L20, Neugrin, Ribosomal_L29, UPF0122
`ENSMUSG00000027012`	Dync1i2	Cytoplasmic dynein 1 intermediate chain 2	2	2	1	15.0	Ref	ANAPC4_WD40, DUF6465, Dynein_IC2, FYDLN_acid, SOBP, WD40
`ENSMUSG00000026767`	Mindy3	Ubiquitin carboxyl-terminal hydrolase MINDY-3	6	2	1	15.0	Mixed	EF-hand_7, MINDY-3_4_CD
`ENSMUSG00000029363`	Rfc5	Replication factor C subunit 5	2	2	1	7.5	Ref	AAA, AAA_11, AAA_14, AAA_16, AAA_19, AAA_22, AAA_24, AAA_25, AAA_3, AAA_30, AAA_5, AAA_7, AAA_assoc_2, ATPase_2, DEAD, DNA_pol3_delta, DNA_pol3_delta2, DUF815, Mg_chelatase, PhoH, RNA_helicase, Rad17, Rep_fac_C, ResIII, RuvB_N, T2SSE, TIP49, TniB, bpMoxR
`ENSMUSG00000025867`	CPLX2	Complexin-2	2	2	1	450.8	Novel	AP-5_subunit_s1, Borrelia_P83, DDHD, DUF2838, DUF4407, MDM10, RNA_polI_A34, RR_TM4-6, Spt5_N, Synaphin, VIT1
`ENSMUSG00000044573`	Acp1	Low molecular weight phosphotyrosine protein phosphatase	2	2	1	5.0	Ref	LMWPc, MmeI_Mtase, TMEM206
`ENSMUSG00000004933`	Matk	Megakaryocyte-associated tyrosine-protein kinase	4	2	1	112.7	Ref	APH, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, RIO1, SH2, SH3_1, SH3_2, SH3_9
`ENSMUSG00000013698`	Pea15	Astrocytic phosphoprotein PEA-15	3	2	1	102.7	Mixed	DED
`ENSMUSG00000021916`	Glt8d1	Glycosyltransferase 8 domain-containing protein 1	2	2	1	10.0	Ref	Glyco_transf_24, Glyco_transf_8, Trehalose_recp
`ENSMUSG00000036225`	KCTD1	BTB/POZ domain-containing protein KCTD1	2	2	1	40.1	Ref	BTB_2, DUF3504, Phage_int_SAM_1
`ENSMUSG00000020925`	Ccdc43	Coiled-coil domain-containing protein 43	2	2	1	47.6	Ref	RepL, SPECT1, TetR_C_28
`ENSMUSG00000025137`	Pcyt2	Ethanolamine-phosphate cytidylyltransferase	2	2	1	117.7	Novel	CTP_transf_like, DUF218, FAD_syn, Oxidored_molyb, ScdA_N
`ENSMUSG00000003721`	Insig2	Insulin-induced gene 2 protein	2	2	1	42.6	Novel	DUF202, DUF2895, INSIG
`ENSMUSG00000026027`	Stradb	STE20-related kinase adapter protein beta	2	2	1	40.1	Ref	DUF1824, ISET-FN3_linker, Kinase-like, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000006463`	Zdhhc24	Probable palmitoyltransferase ZDHHC24	2	2	1	7.5	Ref	CASP_dom, DHHC, Herpes_LMP2
`ENSMUSG00000011114`	Tbrg1	Transforming growth factor beta regulator 1	2	2	1	32.6	Ref	FYRC, FYRN
`ENSMUSG00000024132`	Eci1	Enoyl-CoA delta isomerase 1, mitochondrial	3	2	1	17.5	Mixed	ECH_1, ECH_2, Gasdermin_C
`ENSMUSG00000062270`	Morf4l1	Mortality factor 4-like protein 1	3	2	1	122.7	Novel	MRG, Tudor-knot
`ENSMUSG00000060743`	His3.3A	Histone H3.3A	2	2	1	190.3	Ref	CBFD_NFYB_HMF, CENP-S, CENP-T_C, GHL13, Histone, PAF
`ENSMUSG00000033020`	Polr2f	DNA-directed RNA polymerases I, II, and III subunit RPABC2	2	2	1	227.9	Ref	BDHCT_assoc, RNA_pol_Rpb6
`ENSMUSG00000058444`	Map2k5	Dual specificity mitogen-activated protein kinase kinase 5	2	2	1	17.5	Novel	ABC1, APH, Kinase-like, PB1, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SAM_KSR1, Seadorna_VP7
`ENSMUSG00000023345`	Poc1a	POC1 centriolar protein homolog A	3	2	1	27.5	Novel	WD40
`ENSMUSG00000006476`	Nsmf	NMDA receptor synaptonuclear signaling and neuronal migration factor	2	2	1	47.6	Mixed	DUF4347, IQ
`ENSMUSG00000041645`	Ddx24	ATP-dependent RNA helicase DDX24	3	2	1	62.6	Ref	AAA, AAA_22, DEAD, Helicase_C, PDE4_UCR, ResIII, Tti2
`ENSMUSG00000028351`	Brinp1	BMP/retinoic acid-inducible neural-specific protein 1	2	2	1	250.4	Novel	BRINP, MACPF
`ENSMUSG00000031347`	Cetn2	Centrin-2	2	2	1	47.6	Ref	Asp-B-Hydro_N, CNNM, Caleosin, DUF4601, DUF6694, Dockerin_1, EF-hand_1, EF-hand_11, EF-hand_14, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, Fin, HerA_C, LETM1_RBD, RTP, SRI, SUB1_ProdP9, SurA_N_2, SurA_N_3
`ENSMUSG00000060038`	Dhps	Deoxyhypusine synthase	2	2	1	55.1	Ref	Carbam_trans_N, DS
`ENSMUSG00000033735`	Spr	Sepiapterin reductase	2	2	1	187.8	Ref	KR, Nodulin-like, adh_short, adh_short_C2
`ENSMUSG00000034194`	R3hcc1	R3H and coiled-coil domain-containing protein 1	2	2	1	30.1	Ref	BUD22, CSRNP_N, DUF4407, Dicty_REP, NCBP3, R3H, SDA1
`ENSMUSG00000028991`	Mtor	Serine/threonine-protein kinase mTOR	2	2	1	22.5	Ref	ANAPC3, Adaptin_N, DUF3385, EPSP_synthase, FAT, FATC, FRB_dom, HEAT, HEAT_2, HEAT_EZ, Nipped-B_C, PI3_PI4_kinase, TAN, TPR_19, UME, V-ATPase_H_N
`ENSMUSG00000024194`	Cuta	Protein CutA	7	2	1	288.0	Mixed	CutA1, DAHP_synth_1
`ENSMUSG00000030815`	Phkg2	Phosphorylase b kinase gamma catalytic chain, liver/testis isoform	3	2	1	30.1	Novel	ABC1, APH, Choline_kinase, DUF2805, Haspin_kinase, Kdo, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Pox_ser-thr_kin, RIO1, TCAD9, YrbL-PhoP_reg
`ENSMUSG00000020183`	Cpm	Carboxypeptidase M	2	2	1	5.0	Ref	AstE_AspA, CarboxypepD_reg, DEFB136, Dioxygenase_C, Peptidase_M14
`ENSMUSG00000022151`	Ttc33	Tetratricopeptide repeat protein 33	3	2	1	50.1	Ref	ATG14, ChAPs, TPR_1, TPR_11, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_9
`ENSMUSG00000031154`	Otud5	OTU domain-containing protein 5	2	2	1	5.0	Ref	Hce2, NumbF, OTU, Peptidase_C65, SUKH-4
`ENSMUSG00000020608`	Smc6	Structural maintenance of chromosomes protein 6	2	2	1	5.0	Ref	AAA_15, AAA_18, AAA_21, AAA_23, AAA_27, AAA_29, ABC_tran, DUF3993, DUF5798, Exonuc_VII_L, FtsK_SpoIIIE, SMC_N, TPR_MLP1_2
`ENSMUSG00000015149`	Sirt2	NAD-dependent protein deacetylase sirtuin-2	3	2	1	250.4	Ref	RecQ_Zn_bind, SIR2, YdjO
`ENSMUSG00000024617`	CAMK2A	Calcium/calmodulin-dependent protein kinase type II subunit alpha	2	2	1	160.3	Ref	APH, CaMKII_AD, DUF4440, DUF6590, FTA2, Kdo, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SnoaL_2, SnoaL_3, SnoaL_4
`ENSMUSG00000044475`	Ascc1	Activating signal cointegrator 1 complex subunit 1	3	2	1	22.5	Novel	AKAP7_NLS, KH_1, TraC_F_IV
`ENSMUSG00000074247`	Dda1	DET1- and DDB1-associated protein 1	4	2	1	47.6	Ref	DDA1
`ENSMUSG00000024826`	Dpf2	Zinc finger protein ubi-d4	2	2	1	20.0	Ref	C1_1, DPF1-3_N, Lar_restr_allev, PHD, PHD_2, PHD_4, zf-C2H2, zf-C2H2_4, zf-H2C2_2, zf-PHD-like
`ENSMUSG00000041765`	Ubac2	Ubiquitin-associated domain-containing protein 2	2	2	1	27.5	Novel	FeoA, HA, Rhomboid, TMEM239, UBA
`ENSMUSG00000021660`	Btf3	Transcription factor BTF3	2	2	1	716.2	Ref	LolA, Myco_19_kDa, NAC
`ENSMUSG00000025781`	Atp5f1c	ATP synthase F(1) complex subunit gamma, mitochondrial	3	2	1	1,627.8	Ref	ACT_4, ATP-synt
`ENSMUSG00000024352`	Spata24	Spermatogenesis-associated protein 24	2	2	1	17.5	Ref	DUF4335, DUF4464, ERM_helical, Fez1, Fib_alpha, LMBR1, Lebercilin, SPATA24
`ENSMUSG00000033595`	Lgi3	Leucine-rich repeat LGI family member 3	2	2	1	60.1	Ref	EPTP, LRRCT, LRR_1, LRR_4, LRR_5, LRR_8, LRR_9, RCDG1, stn_TNFRSF12A
`ENSMUSG00000008301`	Phax	Phosphorylated adapter RNA export protein	2	2	1	20.0	Ref	DUF4692, PHAX_RNA-bd
`ENSMUSG00000025534`	Gusb	Beta-glucuronidase	2	2	1	17.5	Ref	Glyco_hydro_2, Glyco_hydro_2_C, Glyco_hydro_2_N
`ENSMUSG00000036242`	Armh4	Armadillo-like helical domain-containing protein 4	2	2	1	5.0	Ref	ARMH4, Autophagy_act_C, BUD22, CDC45, Csc2, DUF2428, FAM176, FYDLN_acid, MLANA, NOA36, Nop14, PI3K_1B_p101, RPA43_OB, Sigma70_ner, TFIIA, TRAP_alpha
`ENSMUSG00000017721`	Pigt	GPI-anchor transamidase component PIGT	2	2	1	15.0	Ref	Gpi16, PRIMA1
`ENSMUSG00000029471`	Camkk2	Calcium/calmodulin-dependent protein kinase kinase 2	2	2	1	32.6	Ref	ABC1, APH, DUF4010, HAND, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Seadorna_VP7
`ENSMUSG00000020773`	Trim47	E3 ubiquitin-protein ligase TRIM47	2	2	1	7.5	Ref	FAP, PRY, Prok-RING_4, SPRY, UBZ_FAAP20, zf-B_box, zf-C2H2_4, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Nse, zf-RING_11, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000061613`	U2AF1	Splicing factor U2AF 35 kDa subunit	3	2	1	237.9	Ref	KMP11, Nup35_RRM_2, RRM_1, Torus, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf_CCCH_4
`ENSMUSG00000015217`	Hmgb3	High mobility group protein B3	3	2	1	47.6	Ref	CHDNT, CLEC16A_C, HMG_box, HMG_box_2, HMG_box_5, RNA_pol_3_Rpc31, Ribosomal_60s, YABBY
`ENSMUSG00000060036`	Rpl3	Large ribosomal subunit protein uL3	2	2	1	2,248.9	Ref	DUF6709, Ribosomal_L3
`ENSMUSG00000053119`	Chmp3	Charged multivesicular body protein 3	2	2	1	85.1	Novel	DUF3915, PSD4, SPOUT_MTase, Snf7
`ENSMUSG00000024792`	Zfpl1	Zinc finger protein-like 1	2	2	1	80.1	Ref	DUF35_N, PHD, PHD_4, zf-4CXXC_R1, zf-B_box, zf-RING_16, zf-RING_2
`ENSMUSG00000030621`	Me3	NADP-dependent malic enzyme, mitochondrial	3	2	1	12.5	Novel	Malic_M, malic
`ENSMUSG00000031765`	MT1G	Metallothionein-1G	2	2	1	1,562.7	Ref	Metallothio, SOXp
`ENSMUSG00000033434`	Gtpbp6	Putative GTP-binding protein 6	3	2	1	12.5	Novel	AAA_16, AAA_23, ABC_tran, DO-GTPase2, Dynamin_N, FeoB_N, GTP-bdg_M, GTP-bdg_N, HflX_C, MMR_HSR1, MobB, PduV-EutP, Rad17, TniB
`ENSMUSG00000008348`	Ubc	Polyubiquitin-C	4	2	1	145.3	Novel	Big_7, CFAP298, CorA, Crinkler, DUF1942, DUF2604, DUF3861, DUF5450, DUF6393, Dsc3_N, FlgD_ig, HemeBinding_Shp, LBP_C, ORF11CD3, Plexin_RBD, ProRS-C_2, Pullulanase_N2, Rad60-SLD, Rad60-SLD_2, Staphylokinase, TBK1_ULD, TmoB, UDP-g_GGTase, Ubiquitin_2, Ubiquitin_4, Ubiquitin_5, ubiquitin
`ENSMUSG00000022415`	Syngr1	Synaptogyrin-1	2	2	1	220.4	Ref	Col_cuticle_N, DUF4064, DUF805, Fig1, MARVEL, Ost5
`ENSMUSG00000029359`	Tesc	Calcineurin B homologous protein 3	2	2	1	180.3	Ref	EF-hand_1, EF-hand_2, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, GIT_CC, M16C_assoc
`ENSMUSG00000031622`	Sin3b	Paired amphipathic helix protein Sin3b	2	2	1	77.6	Ref	DUF5077, PAH, Sin3_corepress, Sin3a_C
`ENSMUSG00000058587`	Tmod3	Tropomodulin-3	2	2	1	12.5	Ref	LRR_6, Tropomodulin, Usher
`ENSMUSG00000057897`	Camk2b	Calcium/calmodulin-dependent protein kinase type II subunit beta	2	2	1	240.4	Novel	APH, CaMKII_AD, DUF4440, FTA2, Kdo, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SnoaL_2, SnoaL_3, SnoaL_4
`ENSMUSG00000006058`	Snf8	Vacuolar-sorting protein SNF8	2	2	1	140.2	Novel	DUF4896, EAP30, EutK_C, Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C, ZapD
`ENSMUSG00000025591`	Tma16	Translation machinery-associated protein 16	2	2	1	7.5	Ref	DUF1910, Tma16
`ENSMUSG00000019188`	Hm13	Signal peptide peptidase	3	2	1	65.1	Ref	DUF4345, Peptidase_A22B, Presenilin, SPP
`ENSMUSG00000028411`	Aptx	Aprataxin	4	2	1	22.5	Ref	CBP_BcsO, DcpS_C, FHA, FHA_2, Glycoamylase, HIT, TAF8_C, zf-AN1, zf-C2H2_4, zf-C2H2_6, zf-C2HE
`ENSMUSG00000034744`	Nagk	N-acetyl-D-glucosamine kinase	4	2	1	57.6	Novel	BcrAD_BadFG, MscS_TM
`ENSMUSG00000035632`	Cnot3	CCR4-NOT transcription complex subunit 3	2	2	1	42.6	Ref	Cluap1, DUF2433, DUF4407, FlxA, Glyco_hydro_67C, NOT2_3_5, Not3, PilJ, Swi5
`ENSMUSG00000019302`	Atp6v0a1	V-type proton ATPase 116 kDa subunit a 1	2	2	1	27.5	Ref	AlkA_N, CCDC85, V_ATPase_I
`ENSMUSG00000025408`	Ddit3	DNA damage-inducible transcript 3 protein	2	2	1	165.3	Novel	CDC45, CLZ, DASH_Hsk3, DASH_Spc19, DDHD, DUF1003, DUF4407, DUF5094, DUF5595, DivIC, Fungal_TACC, GAS, Gcd10p, GrpE, Neur_chan_memb, PRKG1_interact, Phlebovirus_NSM, Presenilin, RR_TM4-6, SWIRM-assoc_2, WDCP, bZIP_1, bZIP_2, eIF3_subunit
`ENSMUSG00000039917`	Rhbdd2	Rhomboid domain-containing protein 2	2	2	1	10.0	Ref	DER1, DUF2070, Rhomboid
`ENSMUSG00000056121`	Fez2	Fasciculation and elongation protein zeta-2	3	2	1	47.6	Ref	DUF5102, FEZ, FapA, Gluconate_2-dh3, MRP-L46, NPV_P10, V_ATPase_I
`ENSMUSG00000022681`	Ntan1	Protein N-terminal asparagine amidohydrolase	8	2	1	95.2	Novel	FIST, N_Asn_amidohyd
`ENSMUSG00000028910`	Mecr	Enoyl-[acyl-carrier-protein] reductase, mitochondrial	2	2	1	27.5	Ref	ADH_N, ADH_zinc_N, ADH_zinc_N_2
`ENSMUSG00000033430`	Terf2ip	Telomeric repeat-binding factor 2-interacting protein 1	2	2	1	22.5	Ref	BRCT_2, DUF4366, HATPase_c_2, Myb_DNA-bind_2, Myb_DNA-bind_6, Rap1_C
`ENSMUSG00000024220`	Znf76	Zinc finger protein 76	2	2	1	15.0	Ref	CFSR, CopK, CpXC, FOXP-CC, FYDLN_acid, Piezo_RRas_bdg, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_aberr, zf-C2H2_jaz, zf-DNA_Pol, zf-Di19, zf-H2C2_2, zf-H2C2_5, zf-TRAF, zf-met, zf_ZIC
`ENSMUSG00000035521`	Gnptg	N-acetylglucosamine-1-phosphotransferase subunit gamma	5	2	1	100.2	Novel	DPM3, PRKCSH_1
`ENSMUSG00000025647`	Shisa5	Protein shisa-5	3	2	1	20.0	Ref	MLANA, Shisa
`ENSMUSG00000037902`	Sirpa	Tyrosine-protein phosphatase non-receptor type substrate 1	2	2	1	35.1	Ref	C1-set, C2-set_2, DUF6479, I-set, Ig_2, Ig_3, MAT1-1-2, SIT, Ureide_permease, V-set, ig
`ENSMUSG00000022962`	Gart	Trifunctional purine biosynthetic protein adenosine-3	3	2	1	5.0	Ref	AIRS, AIRS_C, ATP-grasp, ATP-grasp_2, ATP-grasp_3, ATP-grasp_4, CPSase_L_D2, Dala_Dala_lig_C, Formyl_trans_N, GARS_A, GARS_C, GARS_N, RimK
`ENSMUSG00000028034`	Fubp1	Far upstream element-binding protein 1	2	2	1	37.6	Ref	DUF1897, KH_1, KH_2, KH_4, KH_5, NAD_kinase_C, SH3_12, Secretin_N
`ENSMUSG00000021040`	Slirp	SRA stem-loop-interacting RNA-binding protein, mitochondrial	2	2	1	20.0	Ref	Nup35_RRM, Nup35_RRM_2, PB1, RRM_1, SelR
`ENSMUSG00000063802`	Hspbp1	Hsp70-binding protein 1	3	2	1	167.8	Novel	Arm, CLIP_SPH_mas, Fes1, HEAT, HEAT_EZ, PPTA, Rcd1
`ENSMUSG00000029712`	ACTL6B	Actin-like protein 6B	3	2	1	155.3	Novel	Actin, MobA_MobL, MreB_Mbl
`ENSMUSG00000024175`	Tekt4	Tektin-4	2	2	1	7.5	Ref	APG6_N, ATG16, BORCS8, BST2, Baculo_PEP_C, CLZ, DUF1664, DUF2730, DivIC, EBP50_C, Mating_N, Med9, MitMem_reg, Noelin-1, PI3K_P85_iSH2, Prefoldin, Spc24, TMF_DNA_bd, Tektin, Uso1_p115_C, XhlA
`ENSMUSG00000036748`	Cuedc2	CUE domain-containing protein 2	7	2	1	235.4	Mixed	CUE, DUF6592
`ENSMUSG00000030605`	Mfge8	Lactadherin	2	2	1	293.0	Ref	EGF, EGF_2, F5_F8_type_C, Laminin_EGF, Muskelin_N, cEGF, hEGF
`ENSMUSG00000029780`	Nt5c3a	Cytosolic 5'-nucleotidase 3A	2	2	1	37.6	Ref	ACT_7, CIDE-N, DZR_2, HAD, Hydrolase, UMPH-1, zf-ANAPC11
`ENSMUSG00000019923`	Zwint	Outer kinetochore KNL1 complex subunit ZWINT	3	2	1	1,292.2	Mixed	HEF_HK, Noelin-1, Nuf2_DHR10-like, Zwint
`ENSMUSG00000060126`	Tpt1	Translationally-controlled tumor protein	2	2	1	290.5	Novel	DUF6613, TCTP
`ENSMUSG00000006304`	Arpc2	Actin-related protein 2/3 complex subunit 2	2	2	1	270.5	Novel	ARPC4, Calici_coat, P34-Arc, Vps4_C
`ENSMUSG00000027206`	COPS2	COP9 signalosome complex subunit 2	2	2	1	35.1	Ref	FliS, PCI, TPR_1, TPR_2, TPR_7, YozE_SAM_like
`ENSMUSG00000054766`	Set	Protein SET	2	2	1	20.0	Ref	DUF6279, EMP24_GP25L, Mst1_SARAH, NAP, Nore1-SARAH, OmpH, Phage_Mu_Gam, ZapB
`ENSMUSG00000040888`	Dst	Dystonin	2	2	1	12.5	Ref	APOBEC4, DUF5344, DUF6730, Evr1_Alr, FAD_SOX, GrpE, NCKAP5, Nbl1_Borealin_N, SNAD4, Spectrin
`ENSMUSG00000066456`	Hmgn3	High mobility group nucleosome-binding domain-containing protein 3	2	2	1	235.4	Ref	HMG14_17, TT_ORF2, UPF0239
`ENSMUSG00000002767`	Mrpl2	Large ribosomal subunit protein uL2m	2	2	1	135.2	Ref	Ribosomal_L2, Ribosomal_L2_C
`ENSMUSG00000026019`	Wdr12	Ribosome biogenesis protein WDR12	2	2	1	7.5	Ref	ANAPC4_WD40, DUF6739, NBCH_WD40, NLE, NRDE-2, NUDIX_4, Pap_E4, Pneumo_att_G, RR_TM4-6, Serinc, WD40, Zip
`ENSMUSG00000030401`	Rtn2	Reticulon-2	2	2	1	37.6	Ref	Babuvirus_MP, DUF592, Reticulon, mono-CXXC
`ENSMUSG00000027215`	Cd82	CD82 antigen	2	2	1	35.1	Ref	DUF1310, DUF2070, DUF2157, DUF2981, DUF6057, OAD_gamma, Tetraspanin, Tmemb_55A
`ENSMUSG00000032121`	Tmem218	Transmembrane protein 218	2	2	1	7.5	Novel	CbiQ, DUF4395, DUF6418
`ENSMUSG00000035297`	Cops4	COP9 signalosome complex subunit 4	2	2	1	185.3	Novel	CSN4_RPN5_eIF3a, DinB, PCI, RPN7, TPR_12, TPR_14, TPR_7, TPR_8, YabA
`ENSMUSG00000034403`	Pja1	E3 ubiquitin-protein ligase Praja-1	3	2	1	87.7	Ref	FANCL_C, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000045128`	Rpl18a	Large ribosomal subunit protein eL20	3	2	1	67.6	Ref	Ribosomal_L18A
`ENSMUSG00000052926`	Rnaseh2a	Ribonuclease H2 subunit A	2	2	1	25.0	Ref	ALG11_N, PMSI1, RNase_HII, TSP_1
`ENSMUSG00000048967`	Yjefn3	YjeF N-terminal domain-containing protein 3	2	2	1	25.0	Novel	DUF4381, GRIM-19, Planc_extracel, YjeF_N
`ENSMUSG00000025041`	Nt5c2	Cytosolic purine 5'-nucleotidase	2	2	1	5.0	Ref	5_nucleotid, BCAS2, DUF2095, Filament, Filament_head, bZIP_2
`ENSMUSG00000027333`	Smox	Spermine oxidase	2	2	1	15.0	Ref	3HCDH_N, AlaDh_PNT_C, Amino_oxidase, DAO, FAD_binding_2, FAD_binding_3, FAD_oxidored, FMO-like, GIDA, Glu_dehyd_C, HI0933_like, Lycopene_cycl, MCRA, MLTD_N, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Shikimate_DH, Thi4, Trp_halogenase
`ENSMUSG00000004070`	Hmox2	Heme oxygenase 2	2	2	1	60.1	Ref	CENP-H, DUF349, Heme_oxygenase, SsgA
`ENSMUSG00000024926`	Kat5	Histone acetyltransferase KAT5	4	2	1	32.6	Mixed	Acetyltransf_1, Acetyltransf_7, MOZ_SAS, Tudor-knot, zf-MYST
`ENSMUSG00000030032`	Wdr54	WD repeat-containing protein 54	2	2	1	30.1	Ref	ANAPC4_WD40, Anillin, Lgl_C, PQQ, RAB3GAP2_N, WD40
`ENSMUSG00000005362`	Crbn	Protein cereblon	2	2	1	17.5	Ref	Baculo_ME53, LON_substr_bdg, Yippee-Mis18
`ENSMUSG00000035969`	Rusc2	AP-4 complex accessory subunit RUSC2	2	2	1	25.0	Novel	RUN, SH3_1, SH3_2, SH3_9
`ENSMUSG00000044533`	Rps2	Small ribosomal subunit protein uS5	3	2	1	97.7	Novel	Ribosomal_S5, Ribosomal_S5_C
`ENSMUSG00000038506`	Dcun1d2	DCN1-like protein 2	2	2	1	7.5	Ref	Cullin_binding, TAP_C, TrmE_N, UBA_4
`ENSMUSG00000027282`	Mtch2	Mitochondrial carrier homolog 2	4	2	1	273.0	Ref	Mito_carr
`ENSMUSG00000015672`	Mrpl32	Large ribosomal subunit protein bL32m	2	2	1	17.5	Ref	Caa3_CtaG, DUF5347, DZR, NRDD, RecR, Ribosomal_L32p, UPF0547, zf-RING_7, zf-ribbon_3
`ENSMUSG00000021759`	Plpp1	Phospholipid phosphatase 1	2	2	1	25.0	Novel	DUF418, DUF6541, PAP2, PAP2_C
`ENSMUSG00000021578`	Lrrc14b	Leucine-rich repeat-containing protein 14B	2	2	1	27.5	Ref	ATG14, CT_C_D, CxC2, Exonuc_VII_L, FAP206, LRR_1, LRR_4, LRR_6, LRR_8, Mit_KHE1, Muted, PIN_3, TMPIT, Tuberin
`ENSMUSG00000035215`	Lsm7	U6 snRNA-associated Sm-like protein LSm7	3	2	1	195.3	Novel	LSM
`ENSMUSG00000021750`	Fam107a	Actin-associated protein FAM107A	4	2	1	7.5	Novel	DUF1151, KIAA1328, RNase_Y_N
`ENSMUSG00000026254`	Eif4e2	Eukaryotic translation initiation factor 4E type 2	5	2	1	37.6	Ref	FlgD, IF4E, IHABP4_N, Stm1_N
`ENSMUSG00000033701`	Acbd6	Acyl-CoA-binding domain-containing protein 6	3	2	1	72.6	Ref	ACBP
`ENSMUSG00000006412`	Pfdn2	Prefoldin subunit 2	2	2	1	85.1	Novel	APG6_N, DUF4795, DegS, DivIVA, HalX, Ku_C, Med9, OmpH, Prefoldin, Prefoldin_2, Sigma70_r1_1, Spc24, TPD52
`ENSMUSG00000026095`	Asnsd1	Asparagine synthetase domain-containing protein 1	2	2	1	27.5	Ref	Asn_synthase, GATase_6, GATase_7, NAD_synthase, Prefoldin_2
`ENSMUSG00000026888`	Grb14	Growth factor receptor-bound protein 14	2	2	1	167.8	Novel	BPS, PH, PH_20, RA, SH2
`ENSMUSG00000054021`	Sirt5	NAD-dependent protein deacylase sirtuin-5, mitochondrial	3	2	1	20.0	Ref	GlgS, PNTB, RseA_N, SIR2
`ENSMUSG00000031913`	Vps4a	Vacuolar protein sorting-associated protein 4A	2	2	1	105.2	Novel	AAA, AAA_14, AAA_16, AAA_17, AAA_18, AAA_2, AAA_22, AAA_24, AAA_25, AAA_30, AAA_33, AAA_5, AAA_7, AAA_lid_3, ATPase, ATPase_2, DUF5906, DUF815, EF-1_beta_acid, IstB_IS21, MCM, MIT, MMR_HSR1, Mg_chelatase, NACHT, Parvo_NS1, PhoH, RNA_helicase, RuvB_N, Sigma54_activ_2, Sigma54_activat, TIP49, TPR_2, TniB, Vps4_C, YhdB, bpMoxR
`ENSMUSG00000041278`	Ttc1	Tetratricopeptide repeat protein 1	2	2	1	147.8	Ref	MG2, RPN7, SOAR, TPR_1, TPR_11, TPR_12, TPR_14, TPR_15, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9, eIF3_N
`ENSMUSG00000020585`	Laptm4a	Lysosomal-associated transmembrane protein 4A	2	2	1	606.0	Novel	Circovir2_Orf4, DUF4728, Mtp
`ENSMUSG00000006675`	P4htm	Transmembrane prolyl 4-hydroxylase	2	2	1	135.2	Novel	2OG-FeII_Oxy_3, EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, TruB_C
`ENSMUSG00000030869`	Ndufab1	Acyl carrier protein, mitochondrial	3	2	1	90.2	Mixed	PP-binding, PP-binding_2
`ENSMUSG00000039233`	Tbce	Tubulin-specific chaperone E	2	2	1	20.0	Ref	CAP_GLY, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, Ubiquitin_2
`ENSMUSG00000015087`	Rabl6	Rab-like protein 6	2	2	1	70.1	Novel	Arf, CDC45, CIS_TMP, DDHD, DUF1764, DUF5427, EOS1, MCM_bind, MMR_HSR1, Mitofilin, NatA_aux_su, Neur_chan_memb, Pox_Ag35, Presenilin, RNA_polI_A34, RNA_pol_Rpc4, RR_TM4-6, Ras, Roc, Rrn6, SAPS, SLC12, SpoIIP, Tim54, VirE, eIF3_subunit
`ENSMUSG00000021728`	Emb	Embigin	2	2	1	87.7	Ref	I-set, Ig_2, Ig_3, Ig_4, V-set, ig, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING_11, zf-RING_2, zf-RING_5, zf-rbx1
`ENSMUSG00000060450`	Rnf14	E3 ubiquitin-protein ligase RNF14	3	2	1	75.1	Ref	IBR, RWD, TBPIP, zf-C3HC4, zf-RING_11, zf-RING_2, zf-RING_5
`ENSMUSG00000079317`	TRAPPC2	Trafficking protein particle complex subunit 2	2	2	1	87.7	Ref	DUF4984, Sedlin_N, Sybindin
`ENSMUSG00000031097`	Tnni2	Troponin I, fast skeletal muscle	2	2	1	5.0	Ref	DUF2408, DUF5129, DUF919, Prominin, Troponin, Tup_N
`ENSMUSG00000008855`	Hdac5	Histone deacetylase 5	2	2	1	2.5	Ref	DDHD, HDAC4_Gln, Hist_deacetyl, TFIIA
`ENSMUSG00000032534`	Cep63	Centrosomal protein of 63 kDa	2	2	1	0.0	Ref	CEP63, DUF1843, DUF4407, MEF2_binding
`ENSMUSG00000029166`	Mapre3	Microtubule-associated protein RP/EB family member 3	3	2	1	112.7	Novel	CAMSAP_CH, CH, CH_2, EB1
`ENSMUSG00000031916`	Cog8	Conserved oligomeric Golgi complex subunit 8	2	2	1	15.0	Ref	DUF4279, Dor1, Pep_deformylase, Vps51
`ENSMUSG00000057522`	SPOP	Speckle-type POZ protein	2	2	1	15.0	Ref	BACK, BTB, MATH, YhhN
`ENSMUSG00000002395`	Use1	Vesicle transport protein USE1	5	2	1	260.4	Mixed	Adhes-Ig_like, Amnionless, CCB2_CCB4, Collectrin, DUF5383, MCU, MRP-L28, Orf78, Use1
`ENSMUSG00000052428`	TMCO1	Calcium load-activated calcium channel	2	2	1	147.8	Ref	ArsP_1, Connexin, DUF1090, DUF3671, DUF4257, DUF5683, DivIC, EMC3_TMCO1, FUN14, Kiaa1109_N, Nucleoside_tran, SieB, TMPIT, TRAM1, Vpu
`ENSMUSG00000028647`	Mycbp	c-Myc-binding protein	2	2	1	5.0	Ref	CENP-F_N, TSC22, ZapB
`ENSMUSG00000024012`	Mtch1	Mitochondrial carrier homolog 1	5	2	1	82.6	Ref	Mito_carr, YdjM
`ENSMUSG00000032178`	Ilf3	Interleukin enhancer-binding factor 3	2	2	1	25.0	Ref	DND1_DSRM, DZF, dsrm
`ENSMUSG00000043445`	Pgp	Glycerol-3-phosphate phosphatase	2	2	1	40.1	Ref	Asp_Glu_race, HAD_2, Hydrolase, Hydrolase_6, Hydrolase_like, PGP_phosphatase
`ENSMUSG00000049339`	Retreg2	Reticulophagy regulator 2	3	2	1	25.0	Ref	MASE2, Reticulon
`ENSMUSG00000032869`	Psmf1	Proteasome inhibitor PI31 subunit	2	2	1	25.0	Ref	PI31_Prot_C, PI31_Prot_N
`ENSMUSG00000037058`	Paip2	Polyadenylate-binding protein-interacting protein 2	3	2	1	195.3	Novel	PAM2
`ENSMUSG00000059824`	Dbp	D site-binding protein	3	2	1	27.5	Ref	BSD, bZIP_1, bZIP_2, bZIP_Maf
`ENSMUSG00000030083`	Abtb1	Ankyrin repeat and BTB/POZ domain-containing protein 1	2	2	1	35.1	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, BACK, BTB
`ENSMUSG00000058672`	TUBB2A	Tubulin beta-2A chain	2	2	1	1,612.8	Novel	Misat_Tub_SegII, Tubulin, Tubulin_2, Tubulin_3, Tubulin_C
`ENSMUSG00000017493`	Igfbp4	Insulin-like growth factor-binding protein 4	2	2	1	10.0	Novel	IGFBP, Thyroglobulin_1
`ENSMUSG00000076439`	Mog	Myelin-oligodendrocyte glycoprotein	2	2	1	192.8	Novel	Adeno_E4, I-set, Ig_2, Ig_3, Longin, V-set, ig
`ENSMUSG00000027487`	Cdk5rap1	Mitochondrial tRNA methylthiotransferase CDK5RAP1	2	2	1	2.5	Novel	Radical_SAM, TRAM, UPF0004
`ENSMUSG00000022552`	Sharpin	Sharpin	2	2	1	82.6	Ref	Emfourin, FeoB_Cyto, Sharpin_PH, YebG, ubiquitin, zf-RanBP
`ENSMUSG00000025616`	Usp16	Ubiquitin carboxyl-terminal hydrolase 16	2	2	1	25.0	Ref	BRCT_assoc, DUF4638, DUF5442, DapB_C, RbpA, UCH, zf-UBP, zinc_ribbon_10, zinc_ribbon_9
`ENSMUSG00000028111`	Ctsk	Cathepsin K	2	2	1	7.5	Novel	Inhibitor_I29, Peptidase_C1, Peptidase_C1_2, Peptidase_C39_2
`ENSMUSG00000025508`	Rplp2	Large ribosomal subunit protein P2	3	2	1	110.2	Ref	EF-hand_14, NolX, RelA_AH_RIS, Ribosomal_60s, TFIID_30kDa
`ENSMUSG00000020755`	Sap30bp	SAP30-binding protein	2	2	1	15.0	Novel	CCDC84, HCNGP
`ENSMUSG00000015094`	Npdc1	Neural proliferation differentiation and control protein 1	2	2	1	746.3	Ref	LapA_dom, NPDC1
`ENSMUSG00000031860`	Pbx4	Pre-B-cell leukemia transcription factor 4	2	2	1	17.5	Novel	CENP-B_N, Carbpep_Y_N, DUF1479, HTH_3, Homeobox_KN, Homeodomain, ORC3_N, PBC, YdaS_antitoxin
`ENSMUSG00000057506`	Bloc1s2	Biogenesis of lysosome-related organelles complex 1 subunit 2	2	2	1	157.8	Novel	BLOC1_2, CENP-Q, DUF948, DivIC, Fib_alpha, HEF_HK, NPV_P10, ParB, UPF0449, Uso1_p115_C, XhlA, ZapA
`ENSMUSG00000022234`	Cct5	T-complex protein 1 subunit epsilon	2	2	1	77.6	Novel	Cpn60_TCP1, aRib
`ENSMUSG00000035086`	Becn1	Beclin-1	2	2	1	12.5	Ref	ALMT, APG6, APG6_N, BH3, DASH_Hsk3, DUF3450, DUF4164, DUF4200, Exonuc_VII_L, FapA, GAS, HAUS-augmin3, MT, NAPRTase_C, PP1c_bdg, Suppressor_APC, V_ATPase_I, YabA
`ENSMUSG00000022210`	Dhrs4	Dehydrogenase/reductase SDR family member 4	2	2	1	42.6	Ref	DDAH_eukar, DFP, Epimerase, F420_oxidored, KR, YjeF_N, adh_short, adh_short_C2
`ENSMUSG00000039128`	Cdc123	Translation initiation factor eIF2 assembly protein	2	2	1	37.6	Novel	D123, Reg_prop
`ENSMUSG00000021532`	Fastkd3	FAST kinase domain-containing protein 3, mitochondrial	2	2	1	7.5	Novel	FAST_1, FAST_2, RAP
`ENSMUSG00000024308`	Tapbp	Tapasin	2	2	1	5.0	Ref	C1-set, Ig_3, Med5, ig
`ENSMUSG00000024462`	Gabbr1	Gamma-aminobutyric acid type B receptor subunit 1	3	2	1	42.6	Ref	Sushi
`ENSMUSG00000033653`	Vps8	Vacuolar protein sorting-associated protein 8 homolog	2	2	1	10.0	Ref	Clathrin, PHD, Vps8, zf-C3H2C3, zf-C3HC4, zf-C3HC4_2, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000044442`	Hemk2	Methyltransferase HEMK2	2	2	1	7.5	Ref	DUF5429, DUF938, MTS, Methyltransf_11, Methyltransf_12, Methyltransf_16, Methyltransf_24, Methyltransf_25, Methyltransf_31, Methyltransf_33, PCMT, PrmA, UPF0020
`ENSMUSG00000090015`	Znf431	Zinc finger protein 431	2	2	1	7.5	Ref	BDHCT_assoc, C1_1, C1_4, KRAB, XPA_N, ZZ, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-Paramyx-P, zf-trcl, zf_UBZ, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000025967`	Eef1b	Elongation factor 1-beta	2	2	1	85.1	Ref	EF-1_beta_acid, EF1_GNE, GST_C_3, GST_C_6
`ENSMUSG00000013465`	Nelfb	Negative elongation factor B	2	2	1	10.0	Ref	BatA, COBRA1, DDT
`ENSMUSG00000031878`	Nae1	NEDD8-activating enzyme E1 regulatory subunit	2	2	1	72.6	Ref	E1_4HB, SEP, ThiF
`ENSMUSG00000003421`	Nosip	Nitric oxide synthase-interacting protein	2	2	1	55.1	Ref	PWI, Rtf2, U-box, zf-NOSIP, zf-Nse
`ENSMUSG00000062352`	Itgb1bp1	Integrin beta-1-binding protein 1	2	2	1	15.0	Ref	DHOase, ICAP-1_inte_bdg, PID, YCII
`ENSMUSG00000005312`	Ubqln1	Ubiquilin-1	2	2	1	7.5	Ref	CUE, ComX, DnaG_DnaB_bind, Dsc3_N, HOIP-UBA, PDH_C, Rad60-SLD, Rad60-SLD_2, STI1, UBA, Ubiquitin_4, Ubiquitin_5, XPC-binding, YukD, ubiquitin
`ENSMUSG00000037217`	Syn1	Synapsin-1	2	2	1	95.2	Ref	DUF5879, Dala_Dala_lig_C, Synapsin, Synapsin_C, Synapsin_N
`ENSMUSG00000004980`	HNRNPA2B1	Heterogeneous nuclear ribonucleoproteins A2/B1	3	2	1	30.1	Ref	HnRNPA1, OB_RNB, RRM_1, RRM_7
`ENSMUSG00000015714`	Cers2	Ceramide synthase 2	2	2	1	40.1	Novel	Homeodomain, Homez, TRAM1, TRAM_LAG1_CLN8, UPF0767
`ENSMUSG00000003549`	Ercc1	DNA excision repair protein ERCC-1	2	2	1	75.1	Ref	HHH, HHH_2, HHH_5, Rad10, UPF0758_N
`ENSMUSG00000028420`	Tmem38b	Trimeric intracellular cation channel type B	2	2	1	7.5	Ref	Gly_transporter, TRIC, WDCP
`ENSMUSG00000039660`	Spout1	28S rRNA (uridine-N(3))-methyltransferase	2	2	1	25.0	Ref	AP1AR, DUF2514, Methyltrn_RNA_3
`ENSMUSG00000043259`	Fam13c	Protein FAM13C	2	2	1	7.5	Ref	DUF573, Drc1-Sld2, SOGA, SlyX, Tmemb_cc2, betaPIX_CC
`ENSMUSG00000028329`	Xpa	DNA repair protein complementing XP-A cells homolog	2	2	1	7.5	Ref	Ion_trans_N, XPA_C, XPA_N, YhfH, Zn-ribbon_8, zf_C2H2_13
`ENSMUSG00000014195`	Dnajc7	DnaJ homolog subfamily C member 7	3	2	1	160.3	Ref	ANAPC3, Alkyl_sulf_dimr, BTAD, DnaJ, MIT, NFACT-R_1, RPN7, RPT, Rapsyn_N, SHNi-TPR, Sel1, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_3, TPR_4, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000060073`	Psma3	Proteasome subunit alpha type-3	5	2	1	661.1	Ref	Arm-DNA-bind_3, Proteasome
`ENSMUSG00000041477`	Dcp1b	mRNA-decapping enzyme 1B	2	2	1	2.5	Ref	DCP1, DUF3425, WH1, mRNA_decap_C
`ENSMUSG00000042675`	YPEL3	Protein yippee-like 3	4	2	1	508.4	Mixed	RIG-I_C-RD, Yippee-Mis18
`ENSMUSG00000035443`	Thyn1	Thymocyte nuclear protein 1	5	2	1	125.2	Mixed	EVE
`ENSMUSG00000037936`	Scarb1	Scavenger receptor class B member 1	2	2	1	20.0	Ref	CD36, PorA, TMEM51
`ENSMUSG00000027361`	Gabpb1	GA-binding protein subunit beta-1	2	2	1	5.0	Ref	AAA_23, Ank, Ank_2, Ank_3, Ank_4, Ank_5, CCDC84, CREPT, GrpE, LAMTOR5
`ENSMUSG00000025393`	Atp5f1b	ATP synthase F(1) complex catalytic subunit beta, mitochondrial	2	2	1	247.9	Ref	AAA, AAA_16, AAA_19, ABC_tran, ATP-synt_ab, ATP-synt_ab_N, ATPase, ATPase_2, DUF2075, DUF5832, NACHT, NB-ARC, RsgA_GTPase, T3SS_ATPase_C
`ENSMUSG00000051504`	CD33	Myeloid cell surface antigen CD33	3	2	1	10.0	Ref	C2-set_2, DUF2490, I-set, Ig_3, V-set, ig
`ENSMUSG00000031536`	Polb	DNA polymerase beta	4	2	1	92.7	Novel	DNA_pol_B_palm, DNA_pol_B_thumb, NTP_transf_2
`ENSMUSG00000018160`	Med1	Mediator of RNA polymerase II transcription subunit 1	2	2	1	32.6	Ref	Med1, NIF3
`ENSMUSG00000024436`	Mrps18b	Small ribosomal subunit protein mS40	3	2	1	82.6	Mixed	FAM165, Ribosomal_S18
`ENSMUSG00000118346`	Tmem179b	Transmembrane protein 179B	2	2	1	25.0	Novel	CASP_dom, DUF3333, DUF6057, TMEM132D_C
`ENSMUSG00000032172`	OLFM2	Noelin-2	2	2	1	107.7	Ref	ABC_tran_CTD, CLZ, DUF1664, Ephrin, Helo_like_N, Matrilin_ccoil, Noelin-1, OLF, PepSY_2
`ENSMUSG00000017421`	Znf207	BUB3-interacting and GLEBS motif-containing protein ZNF207	2	2	1	5.0	Ref	SSP160, zf-C2H2, zf-C2H2_4, zf-FCS
`ENSMUSG00000026915`	Strbp	Spermatid perinuclear RNA-binding protein	2	2	1	45.1	Ref	DND1_DSRM, DUF936, DZF, Phage_clamp_A, SGP, dsrm
`ENSMUSG00000031060`	Rbm10	RNA-binding protein 10	2	2	1	7.5	Ref	DUF4407, G-patch, G-patch_2, GSIII_N, OCRE, PRE_C2HC, RRM_1, TackOD1, zf-C2H2_4, zf-RanBP
`ENSMUSG00000035478`	Mbd3	Methyl-CpG-binding domain protein 3	3	2	1	45.1	Ref	MBD, MBD_C, MBDa, NUMOD1, RasGAP
`ENSMUSG00000018921`	Pelp1	Proline-, glutamic acid- and leucine-rich protein 1	2	2	1	22.5	Ref	BUD22, NUC202, Nop14, RIX1, Urb2
`ENSMUSG00000029088`	Kcnip4	Kv channel-interacting protein 4	2	2	1	27.5	Ref	Caleosin, DUF3192, Dockerin_1, EF-hand_1, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, SPARC_Ca_bdg
`ENSMUSG00000031837`	Necab2	N-terminal EF-hand calcium-binding protein 2	2	2	1	17.5	Novel	ABM, DUF5790, EF-hand_1, EF-hand_10, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, HAUS-augmin3, SPARC_Ca_bdg, UPF0181
`ENSMUSG00000026270`	Capn10	Calpain-10	2	2	1	20.0	Ref	Calpain_III, Peptidase_C2
`ENSMUSG00000039745`	Htatip2	Protein HTATIP2	2	2	1	5.0	Novel	ANF_receptor, Epimerase, NAD_binding_10, NAD_binding_4, Sacchrp_dh_NADP, Semialdhyde_dh
`ENSMUSG00000074182`	Znhit6	Box C/D snoRNA protein 1	2	2	1	5.0	Ref	PolC_DP2, zf-B_box, zf-HIT
`ENSMUSG00000000308`	Ckmt1	Creatine kinase U-type, mitochondrial	3	2	1	270.5	Novel	ATP-gua_Ptrans
`ENSMUSG00000024180`	Pgap6	Post-GPI attachment to proteins factor 6	2	2	1	5.0	Ref	DUF3522, LtrA, SLATT_3
`ENSMUSG00000019738`	POLR2I	DNA-directed RNA polymerase II subunit RPB9	2	2	1	105.2	Ref	CpXC, DUF3716, DZR, Ogr_Delta, RNA_POL_M_15KD, TFIIS_C, TF_Zn_Ribbon, Zn-ribbon_8, Zn_Tnp_IS1595, Zn_Tnp_IS91, Zn_ribbon_recom
`ENSMUSG00000033272`	Slc35a4	Probable UDP-sugar transporter protein SLC35A4	3	2	1	12.5	Novel	DUF4535, EamA, Nuc_sug_transp
`ENSMUSG00000079494`	Nat8f5	Probable N-acetyltransferase family 8 member 5	2	2	1	2.5	Novel	Acetyltransf_1, Acetyltransf_10, Acetyltransf_15, Acetyltransf_3, Acetyltransf_4, Acetyltransf_7, Acetyltransf_8, Acetyltransf_9, Acetyltransf_CG, DUF4131, FR47
`ENSMUSG00000027523`	Gnas	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	2	2	1	505.9	Ref	AAA_29, Arf, G-alpha, GTP_EFTU, Gtr1_RagA, MARVEL, NESP55, Roc, TniB
`ENSMUSG00000064264`	Znf428	Zinc finger protein 428	2	2	1	110.2	Ref	PIEZO, zf-C2H2_4
`ENSMUSG00000010097`	Nxf1	Nuclear RNA export factor 1	2	2	1	17.5	Ref	DUF1319, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, NTF2, TAP_C, Tap-RNA_bind
`ENSMUSG00000001707`	Eef1e1	Eukaryotic translation elongation factor 1 epsilon-1	2	2	1	12.5	Novel	GST_C, GST_C_2, GST_C_3, GST_C_6, Tom37
`ENSMUSG00000031897`	Psmb10	Proteasome subunit beta type-10	3	2	1	205.4	Novel	NAD_Gly3P_dh_C, Pr_beta_C, Proteasome
`ENSMUSG00000035953`	Pip4p1	Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase	3	2	1	40.1	Ref	FlhC, Herpes_US9, Tmemb_55A, zinc_ribbon_12
`ENSMUSG00000035958`	Tdp2	Tyrosyl-DNA phosphodiesterase 2	2	2	1	10.0	Ref	Exo_endo_phos, UBA_4
`ENSMUSG00000036371`	Serbp1	SERPINE1 mRNA-binding protein 1	4	2	1	105.2	Ref	HABP4_PAI-RBP1, IHABP4_N
`ENSMUSG00000059743`	Fdps	Farnesyl pyrophosphate synthase	2	2	1	165.3	Ref	ANIS5_cation-bd, polyprenyl_synt
`ENSMUSG00000039533`	Mmd2	Monocyte to macrophage differentiation factor 2	2	2	1	20.0	Novel	Ceramidase, DUF2842, DUF4199, Ferlin_C, HlyIII
`ENSMUSG00000022856`	Tmem41a	Transmembrane protein 41A	2	2	1	50.1	Ref	SNARE_assoc, ThiW
`ENSMUSG00000027582`	Zgpat	Zinc finger CCCH-type with G patch domain-containing protein	2	2	1	5.0	Ref	ABC_tran_CTD, Agenet, Bacillus_HBL, CCDC-167, CcmH, G-patch, LIME1, PAG, Ribosomal_L29, zf-CCCH, zf-CCCH_2, zf-CCCH_4, zf_CCCH_4
`ENSMUSG00000003873`	Bax	Apoptosis regulator BAX	3	2	1	130.2	Novel	BID, Bcl-2, Bclx_interact, CF222
`ENSMUSG00000021619`	Atg10	Ubiquitin-like-conjugating enzyme ATG10	2	2	1	22.5	Ref	Autophagy_act_C, zinc_ribbon_4
`ENSMUSG00000027397`	Slc20a1	Sodium-dependent phosphate transporter 1	2	2	1	15.0	Ref	DUF2269, PHO4
`ENSMUSG00000079179`	—	—	2	2	2	22.5	Ref	DUF1091, TFIIA
`ENSMUSG00000036372`	TMEM258	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258	2	2	2	182.8	Ref	DUF6512, Ost5, Phage_holin_3_3, RIIa, Xre_MbcA_ParS_C
`ENSMUSG00000050708`	Ftl1	Ferritin light chain 1	2	2	3	260.4	Ref	ANAPC4, Ferritin
`ENSMUSG00000021039`	Snw1	SNW domain-containing protein 1	3	2	4	45.1	Novel	SIR4_SID, SKIP_SNW
`ENSMUSG00000025024`	Smndc1	Survival of motor neuron-related-splicing factor 30	2	2	4	27.5	Ref	ATP_sub_h, SMN, TUDOR
`ENSMUSG00000031169`	Porcn	Protein-serine O-palmitoleoyltransferase porcupine	3	2	4	40.1	Ref	DUF6726, FUSC_2, MBOAT
`ENSMUSG00000055430`	Nap1l5	Nucleosome assembly protein 1-like 5	2	2	4	393.2	Novel	ANAPC15, Ax_dynein_light, Dicty_REP, HrpB7, Mst1_SARAH, NAP, OmpH, Phage_Mu_Gam, Voldacs
`ENSMUSG00000004631`	Sgce	Epsilon-sarcoglycan	3	2	4	30.1	Ref	Sarcoglycan_2
`ENSMUSG00000047459`	DYNLRB1	Dynein light chain roadblock-type 1	2	2	4	1,139.5	Ref	DUF2326, LAMTOR5, PIH1, Robl_LC7, UPF0176_N
`ENSMUSG00000055805`	Fmnl1	Formin-like protein 1	2	2	4	10.0	Novel	FH2, I_LWEQ, TPR_6
`ENSMUSG00000015488`	Cacfd1	Calcium channel flower homolog	2	2	4	25.0	Ref	Bacteriocin_IIc, COPI_assoc, Cg6151-P, DUF4191, TMEM72
`ENSMUSG00000092607`	Scnm1	Sodium channel modifier 1	2	2	4	42.6	Ref	SCNM1_acidic, zf-SCNM1
`ENSMUSG00000056228`	Cars2	Probable cysteine--tRNA ligase, mitochondrial	2	2	4	7.5	Ref	DALR_2, tRNA-synt_1, tRNA-synt_1e, tRNA-synt_1f, tRNA-synt_1g
`ENSMUSG00000022792`	Yars2	Tyrosine--tRNA ligase, mitochondrial	2	2	4	20.0	Ref	DUF4884, S4, tRNA-synt_1b
`ENSMUSG00000063236`	—	UPF0488 protein C8orf33 homolog	2	2	4	32.6	Ref	CtsR_C, DUF4615, PDE4_UCR, PGC7_Stella
`ENSMUSG00000028634`	—	—	2	2	4	70.1	Novel	Activin_recp, SIP1
`ENSMUSG00000028837`	Psmb2	Proteasome subunit beta type-2	2	2	4	463.3	Novel	Proteasome, WSLR
`ENSMUSG00000040462`	Os9	Protein OS-9	2	2	4	77.6	Ref	IL4Ra_N, PRKCSH, PRKCSH_1, RRN3, SNAP-25
`ENSMUSG00000024962`	Vegfb	Vascular endothelial growth factor B	2	2	4	197.8	Ref	CXCXC, PDGF, VEGF_C
`ENSMUSG00000052560`	Cpne8	Copine-8	2	2	4	25.0	Ref	C2, Copine, FG-GAP_3, Glyco_hydro_106, vWA-TerF-like
`ENSMUSG00000059208`	Hnrnpm	Heterogeneous nuclear ribonucleoprotein M	2	2	4	40.1	Ref	Collagen_mid, HnRNP_M_NLS, MARF1_RRM1, RRM_1, RRM_3, RRM_5, RRM_occluded, XS
`ENSMUSG00000022556`	Hsf1	Heat shock factor protein 1	2	2	4	17.5	Ref	HMMR_C, HSF_DNA-bind, NPV_P10, Vert_HS_TF, ZapB
`ENSMUSG00000022822`	Abcc5	ATP-binding cassette sub-family C member 5	2	2	4	20.0	Novel	AAA_13, AAA_16, AAA_18, AAA_21, AAA_22, AAA_23, AAA_24, AAA_29, AAA_7, ABC_membrane, ABC_tran, DO-GTPase2, DUF87, Dynamin_N, LVIVD, MMR_HSR1, NB-ARC, PDGF, RsgA_GTPase, SMC_N, TsaE, Zeta_toxin, cobW
`ENSMUSG00000021606`	Ndufs6	NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial	2	2	4	212.9	Ref	Chitin_synth_2, Thiolase_C, zf-CHCC
`ENSMUSG00000015092`	Edf1	Endothelial differentiation-related factor 1	2	2	4	1,014.3	Novel	HTH_3, HTH_31, MBF1
`ENSMUSG00000026279`	Thap4	Peroxynitrite isomerase THAP4	2	2	4	5.0	Ref	Csm1_N, DUF4201, HrpB7, THAP, THAP4_heme-bd, TMF_DNA_bd, Tropomyosin_1
`ENSMUSG00000121778`	Ube2v1	Ubiquitin-conjugating enzyme E2 variant 1	4	2	4	370.6	Ref	UQ_con
`ENSMUSG00000042729`	Wdr74	WD repeat-containing protein 74	2	2	4	90.2	Ref	ANAPC4_WD40, CCDC106, MraZ, WD40
`ENSMUSG00000002329`	Mdp1	Magnesium-dependent phosphatase 1	2	2	4	82.6	Ref	ABM, Acid_PPase, HAD_2, Hydrolase, NIF
`ENSMUSG00000031708`	Tecr	Very-long-chain enoyl-CoA reductase	3	2	4	1,958.4	Ref	Steroid_dh
`ENSMUSG00000028759`	Hp1bp3	Heterochromatin protein 1-binding protein 3	2	2	4	15.0	Novel	CCD97-like_C, CDC45, EMC7_beta-sandw, Linker_histone, RRN3, SDA1, SURF2, YL1
`ENSMUSG00000028066`	Pmf1	Polyamine-modulated factor 1	2	2	4	22.5	Ref	BLOC1_2, BRI3BP, DUF16, DUF1664, DUF4618, Ku_PK_bind, MnmE_helical, Mod_r, Nnf1, OmpH, QueF, RmuC, SYCP2_ARLD, SlyX, TSC22, TetR_C_4, Uso1_p115_C, Wtap, betaPIX_CC
`ENSMUSG00000092074`	Dynlt1	Dynein light chain Tctex-type 1	3	2	4	7.5	Novel	Tctex-1
`ENSMUSG00000022892`	App	Amyloid-beta precursor protein	2	2	4	966.7	Ref	APP_Cu_bd, APP_E2, APP_N, APP_amyloid, Activator-TraM, Beta-APP, DUF5033, Dicty_REP, Exonuc_VII_L, Insulin_TMD, Kunitz_BPTI, PI3K_1B_p101, PilJ, Prominin, SDA1, Spore_III_AB
`ENSMUSG00000003411`	Rab3b	Ras-related protein Rab-3B	2	2	4	55.1	Ref	AAA_16, AAA_24, Arf, FeoB_N, Fin, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB
`ENSMUSG00000020738`	SUMO2	Small ubiquitin-related modifier 2	2	2	4	1,364.9	Ref	DUF5010_C, Rad60-SLD, ubiquitin
`ENSMUSG00000015291`	Gdi1	Rab GDP dissociation inhibitor alpha	2	2	4	255.4	Novel	Amino_oxidase, GDI, SH3_14
`ENSMUSG00000032551`	—	Uncharacterized protein KIAA1143 homolog	2	2	4	20.0	Ref	DUF4604, DUF4848, Peptidase_S49_N
`ENSMUSG00000031388`	Naa10	N-alpha-acetyltransferase 10	3	2	4	165.3	Ref	Acetyltransf_1, Acetyltransf_10, Acetyltransf_13, Acetyltransf_15, Acetyltransf_3, Acetyltransf_7, Acetyltransf_9, FR47
`ENSMUSG00000024234`	Mtpap	Poly(A) RNA polymerase, mitochondrial	2	2	4	17.5	Ref	Nrap_D2, PAP_assoc, RL, TUTase, tRNA_NucTransf2
`ENSMUSG00000003872`	Lin7b	Protein lin-7 homolog B	2	2	4	190.3	Novel	L27, PDZ, PDZ_2, PDZ_6, Peptidase_M50, RecC_C
`ENSMUSG00000063765`	Chadl	Chondroadherin-like protein	2	2	4	40.1	Ref	LRRCT, LRRNT, LRR_1, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9
`ENSMUSG00000027108`	Ola1	Obg-like ATPase 1	2	2	4	100.2	Ref	AAA_18, ABC_tran, Dynamin_N, FeoB_N, MMR_HSR1, MeaB, Roc, TGS, YchF-GTPase_C
`ENSMUSG00000032966`	Fkbp1a	Peptidyl-prolyl cis-trans isomerase FKBP1A	2	2	4	1,169.5	Ref	FKBP_C, Glyoxalase_4
`ENSMUSG00000027618`	Nfs1	Cysteine desulfurase	2	2	4	55.1	Ref	Aminotran_5, Beta_elim_lyase, DegT_DnrJ_EryC1, EH_Signature, Peptidase_C48, Pyridoxal_deC
`ENSMUSG00000024146`	Cript	Cysteine-rich PDZ-binding protein	2	2	4	263.0	Ref	Cas_CXXC_CXXC, Cript, DUF1272, DUF2039, DZR_2, Fin, Gin, PLATZ, Rhodanese_C, zf-RING_9, zinc_ribbon_2
`ENSMUSG00000032527`	Pccb	Propionyl-CoA carboxylase beta chain, mitochondrial	2	2	4	22.5	Ref	Carboxyl_trans, Lys_Orn_oxgnase, MdcE
`ENSMUSG00000025499`	H-RAS	Transforming protein p29	3	2	4	55.1	Ref	AAA_16, ATP_bind_1, Arf, DEC-1_N, FeoB_N, G-alpha, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, Septin
`ENSMUSG00000019883`	Echdc1	Ethylmalonyl-CoA decarboxylase	2	2	4	0.0	Ref	DUF4478, ECH_1, ECH_2, Helicase_C_4, Sp-DndD, WWE
`ENSMUSG00000015806`	Qdpr	Dihydropteridine reductase	3	2	4	768.8	Novel	Peptidase_S66, adh_short, adh_short_C2
`ENSMUSG00000031585`	Gtf2e2	General transcription factor IIE subunit 2	2	2	4	30.1	Ref	PhoQ_Sensor, TFA2_Winged_2, TFIIE_beta
`ENSMUSG00000027574`	Nkain4	Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4	2	2	4	210.4	Ref	12TM_1, DUF1003, NKAIN
`ENSMUSG00000039148`	Sart1	U4/U6.U5 tri-snRNP-associated protein 1	2	2	4	7.5	Ref	HIND, SART-1, T3SS_needle_E

GO Term Enrichment (QC-filtered domain variant genes, FDR < 0.05)

Overrepresented GO terms in genes with genuine domain architecture variants (after artifact removal). These terms indicate which biological processes, molecular functions, and cellular compartments are enriched for alternative splicing that alters protein domain composition.

All Significant GO Terms

Namespace	GO ID	Term	DV Genes	Background	FDR	Genes
BP	`GO:0006886`	intracellular protein transport	27/557	131/6664	`0.043217`	ARF1, Ap3b1, Ap3m2, Arfip2, Aspscr1, Bcap31, CLTA, Cltb, Copg1, Ift22, Klc1, RAB24, Rab13, Rab18, Rab28, Rab6a, Ramp2, STXBP1, Scamp3, Snapin, Snx17, Snx2, Stx18, Stx4, Stx5, Tmed9, Vti1b

DV Enrichment by Expression Level (CPM from upstream transcriptomics)

Baseline DV Rate

7.3%

566 / 7,727 genes

Peak DV Rate

12.4%

Very high (>=200) bin

Peak Enrichment

1.7×

vs baseline

Chi-square trend

p < 0.001

Expression Bin	Total Genes	DV Genes	DV %	Fold vs Baseline
Silent (<1) CPM	513	6	1.2%	0.2×
Low (1-10) CPM	2,201	67	3.0%	0.4×
Moderate (10-50) CPM	2,839	229	8.1%	1.1×
High (50-200) CPM	1,447	174	12.0%	1.6×
Very high (>=200) CPM	727	90	12.4%	1.7×

Bar height shows the percentage of genes that are domain-variant within each expression bin. Dashed line = overall DV rate (7.3%). If DV enrichment increases monotonically with expression, ascertainment bias (more reads = more isoform discovery) is the dominant driver. Enrichment above baseline at specific expression tiers may indicate biological enrichment beyond the detection effect. CPM from 399,309 mRNA reads (0.1% rRNA excluded).

ORF Type Distribution

NMD Prediction by ORF Type

NMD Candidates by Evidence Tier

NMD Expression Validation (CPM from upstream transcriptomics)

NMD Expression Rate

97.6%

246 / 252

Non-NMD Expression Rate

95.2%

9,391 / 9,862

Fold Difference

1.0×

non-NMD / NMD

Tier 1 NMD Expression

98.9%

91 / 92

NMD Candidates (n=252)

Non-NMD (n=9,862)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual transcript CPM values are overlaid as jittered points. NMD candidates are expressed at 97.6% (median 7.5 CPM) vs 95.2% (median 12.5 CPM) for non-NMD — no evidence of NMD-mediated degradation.

Output Files

blastp.with_title.tsv HPC 1.5 MB gene_functional_summary.tsv HPC 310.7 KB isoform_annotations.tsv HPC 2.2 MB job.yml HPC 608 B pfam.domtblout HPC 21.8 MB results_summary.json HPC 1165.9 KB

Provenance

Execution	Expression quantification summary
Completed	2026-03-01T04:44:15+00:00

RO-Crate 1.1 Workflow RO-Crate 1.0 FAIR

This analysis is packaged as a Research Object Crate with machine-readable provenance and FAIR metadata.

RO-Crate Metadata (JSON-LD)

Show/hide raw JSON-LD

{
    "@context": [
        "https://w3id.org/ro/crate/1.1/context",
        {
            "bioschemas": "https://bioschemas.org/"
        }
    ],
    "@graph": [
        {
            "@id": "ro-crate-metadata.json",
            "@type": "CreativeWork",
            "about": {
                "@id": "./"
            },
            "conformsTo": [
                {
                    "@id": "https://w3id.org/ro/crate/1.1"
                },
                {
                    "@id": "https://w3id.org/workflowhub/workflow-ro-crate/1.0"
                }
            ]
        },
        {
            "@id": "./",
            "@type": "Dataset",
            "name": "ONT Isoform Functional Annotation \u2014 Run #48",
            "description": "Pfam domain search, SwissProt homology, NMD prediction, and functional diversity classification for ONT transcript isoforms.",
            "datePublished": "2026-03-01",
            "license": {
                "@id": "https://creativecommons.org/licenses/by/4.0/"
            },
            "mainEntity": {
                "@id": "ont_isoform_annotation.cwl"
            },
            "hasPart": [
                {
                    "@id": "ont_isoform_annotation.cwl"
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        {
            "@id": "#protocol-42",
            "@type": "LabProtocol",
            "name": "Dissection and collection of tissue from 10-week-old male mice",
            "description": "Surgical dissection and collection of cortex, pituitary, and liver tissues from 10-week-old male mice, performed by Jackson Laboratory surgical team (Bar Harbor, ME, USA). Tissues snap-frozen and shipped internationally to Australia via dry shipper using FedEx.",
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
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                    "text": "# SOP: Dissection and Collection of Tissue from 10-Week-Old Male Mice\n\n**Applies to:** 10-week-old male mice (C57BL/6J, DBA/2J, or other inbred strains)\n**Tissues collected:** Cortex, pituitary, liver\n**Performed by:** Surgical team, The Jackson Laboratory, Bar Harbor, ME, USA\n**Shipping:** Dry shipper via FedEx International Priority to Australia\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nTo obtain high-quality, snap-frozen tissue samples (cortex, pituitary, liver) from 10-week-old male mice for downstream molecular analysis (RNA extraction, DNA extraction, proteomics). Tissues are collected at The Jackson Laboratory and shipped internationally to Australia under cryogenic conditions to preserve nucleic acid and protein integrity.\n\n## Scope\n\nAll personnel involved in the planning, coordination, dissection, tissue collection, and shipment of mouse tissue samples between The Jackson Laboratory (USA) and the receiving laboratory (Australia).\n\n## Responsibilities\n\n* **Principal Investigator (receiving lab):** Define experimental design, strain requirements, tissue list, and sample labelling scheme. Coordinate import permits and institutional approvals.\n* **Jackson Laboratory surgical team:** Perform euthanasia, dissection, tissue collection, snap-freezing, and packaging for shipment.\n* **Shipping coordinator:** Arrange FedEx International Priority shipment, customs documentation, and dry shipper logistics.\n* **Receiving lab personnel:** Receive shipment, verify sample integrity, transfer to long-term storage, and log receipt.\n\n## Regulatory and Ethical Requirements\n\n* All animal procedures must be approved by The Jackson Laboratory IACUC (Institutional Animal Care and Use Committee) under an active protocol.\n* Receiving institution must hold a valid **import permit** for biological materials from the relevant national authority (e.g., Australian Department of Agriculture, Fisheries and Forestry \u2014 DAFF).\n* Ensure compliance with CITES regulations (not typically applicable for laboratory mice, but verify for specific strains).\n* **Biosecurity:** Frozen tissues from SPF (Specific Pathogen Free) mice. Health monitoring reports from Jackson Laboratory should accompany the shipment.\n\n---\n\n## Materials and Equipment\n\n### At Jackson Laboratory (dissection)\n\n* Certified CO\u2082 euthanasia station or injectable anaesthetic (as per IACUC protocol)\n* Cervical dislocation tools (secondary method of euthanasia)\n* Sterile surgical instruments: fine scissors, forceps (curved and straight), scalpel with #10 and #15 blades, micro-dissecting forceps, bone rongeurs (for skull)\n* Sterile surgical drapes and pads\n* Sterile PBS (phosphate-buffered saline), ice-cold\n* Liquid nitrogen in Dewar flask\n* Pre-labelled cryovials (1.5\u20132.0 mL, cryogenic-rated, screw-cap)\n* Cryogenic labels (resistant to LN\u2082 and \u221280 \u00b0C)\n* RNAlater (optional, as backup for tissues not immediately snap-frozen)\n* Absorbent pads and biohazard waste bags\n* PPE: lab coat, gloves, face shield (for LN\u2082 handling)\n\n### For shipping\n\n* **Dry shipper** (vapour-phase liquid nitrogen dewar, e.g., MVE SC 4/2V or Taylor-Wharton CX100)\n* Cryogenic storage canes or racks\n* Secondary containment bags (leak-proof, absorbent material)\n* FedEx International Priority shipping account\n* Customs documentation: commercial invoice, packing list, import permit, health certificate, AQIS declaration\n* Temperature data logger (optional but recommended)\n\n### At receiving laboratory (Australia)\n\n* \u221280 \u00b0C ultra-low freezer with allocated space\n* Cryogenic gloves and face shield\n* Sample inventory system / database\n\n---\n\n## Procedure\n\n### 1. Pre-Dissection Planning\n\n1. Confirm **mouse strain, sex (male), and age (10 weeks \u00b1 2 days)** with Jackson Laboratory.\n2. Provide Jackson Laboratory with:\n   * Number of animals required (biological replicates)\n   * Tissue list: **cortex, pituitary, liver**\n   * Sample labelling scheme (e.g., strain_tissue_replicate: C57_CTX_1, DBA_PIT_3)\n   * Any special handling instructions (e.g., morning collection to control circadian effects)\n3. Confirm **IACUC protocol** is active and covers the planned procedures.\n4. Obtain **Australian import permit** (DAFF) for frozen animal tissues. Allow 4\u20136 weeks lead time.\n5. Arrange **FedEx International Priority** account and confirm dry shipper availability and return logistics.\n\n### 2. Animal Preparation\n\n1. House mice under standard conditions (12 h light/dark cycle, ad libitum food and water, 20\u201324 \u00b0C) for at least 1 week prior to collection to minimise stress-related transcriptomic changes.\n2. Record for each animal:\n   * Strain and stock number\n   * Date of birth and age at collection\n   * Body weight\n   * Cage number and housing conditions\n   * Any health observations or abnormalities\n3. Fast animals for **4\u20136 hours** prior to collection if liver metabolomic consistency is required (optional \u2014 confirm with PI).\n\n### 3. Euthanasia\n\n1. Euthanise by **CO\u2082 inhalation** (gradual displacement, 30\u201370% chamber volume/min) followed by **cervical dislocation** as secondary confirmation, in accordance with AVMA Guidelines and IACUC protocol.\n2. Confirm death: absence of heartbeat, respiration, and pedal reflex.\n3. Record **time of death** \u2014 proceed to dissection immediately (within 5 min) to minimise RNA degradation.\n\n### 4. Tissue Dissection and Collection\n\nPerform all dissections on a clean, sterile surface. Use separate sterile instruments for each tissue to prevent cross-contamination. Work quickly \u2014 total dissection time per animal should be **<15 minutes**.\n\n#### 4a. Brain \u2014 Cortex\n\n1. Decapitate the mouse. Remove the skin overlying the skull.\n2. Using bone rongeurs or fine scissors, carefully open the skull along the sagittal suture from the foramen magnum to the olfactory bulbs.\n3. Gently lift and peel back the skull plates to expose the brain.\n4. Using curved forceps, carefully lift the brain from the cranial cavity, severing cranial nerves and the spinal cord at the brainstem.\n5. Place the brain dorsal-side up on a cold surface (glass plate on ice or cold PBS-dampened filter paper).\n6. Using a scalpel or razor blade, remove the **cerebral cortex** by making a coronal cut posterior to the olfactory bulbs and peeling the cortical hemispheres away from the underlying structures (hippocampus, striatum, thalamus).\n7. Transfer cortex tissue immediately to a **pre-labelled cryovial**.\n8. **Snap-freeze** by plunging the cryovial into liquid nitrogen. Hold submerged for \u226530 seconds.\n\n#### 4b. Pituitary\n\n1. After removing the brain, examine the base of the skull (sella turcica).\n2. The **pituitary gland** is a small (~1 mm), pinkish structure sitting in the sella turcica.\n3. Using fine-tipped forceps, gently tease the pituitary free from the surrounding membrane and bone.\n4. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen immediately.\n5. **Note:** The pituitary is very small \u2014 take care not to lose or fragment it. If it fragments, collect all pieces.\n\n#### 4c. Liver\n\n1. Open the abdominal cavity with a midline ventral incision from pelvis to sternum.\n2. Retract the intestines to expose the liver.\n3. Identify the **left lateral lobe** (largest, most accessible lobe).\n4. Excise a **~50\u2013100 mg piece** (approximately 5 \u00d7 5 \u00d7 3 mm) from the left lateral lobe using a clean scalpel or razor blade.\n5. Briefly blot on sterile filter paper to remove excess blood.\n6. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen.\n7. If the entire liver is required, excise all lobes, record total liver weight, and snap-freeze in separate vials.\n\n### 5. Post-Dissection\n\n1. Transfer all snap-frozen cryovials from liquid nitrogen to a **\u221280 \u00b0C freezer** within 1 hour of collection.\n2. Dispose of carcasses and biological waste according to Jackson Laboratory institutional procedures.\n3. Clean and decontaminate all instruments and work surfaces.\n4. Complete the **dissection log** for each animal:\n\n   | Field | Record |\n   |---|---|\n   | Animal ID | Strain, stock #, ear tag/toe clip |\n   | Date and time of euthanasia | |\n   | Body weight (g) | |\n   | Tissues collected | Cortex, pituitary, liver |\n   | Tissue appearance | Normal / abnormal (describe) |\n   | Cryovial labels | List all vial IDs |\n   | Operator | Name and signature |\n   | Notes | Any deviations or observations |\n\n### 6. Packaging for International Shipment\n\n1. **Charge the dry shipper** with liquid nitrogen at least 24 hours before packing. Drain excess liquid \u2014 the dry shipper must contain only **vapour-phase nitrogen** (no free liquid) for air transport compliance (IATA).\n2. Verify the dry shipper holds temperature below **\u2212150 \u00b0C** and has a rated hold time sufficient for transit (typically 10\u201314 days).\n3. Place cryovials in **cryogenic canes or racks** and insert into the dry shipper.\n4. Seal the dry shipper and place in the **outer shipping container** (insulated box or hard case).\n5. Include **absorbent material** inside the secondary containment bag (regulatory requirement for biological specimens).\n6. Attach a **temperature data logger** (optional but recommended) to monitor transit conditions.\n7. Prepare shipping documentation:\n   * **Commercial invoice** (contents: frozen mouse tissue samples, no commercial value / research use only)\n   * **Packing list** (number of vials, tissue types, strain, animal IDs)\n   * **Australian import permit** (DAFF-issued)\n   * **Health monitoring report** from Jackson Laboratory (SPF status)\n   * **Dangerous goods declaration** \u2014 Class 9 UN3373 Biological Substance Category B (if required by carrier) or exempt if shipped as non-infectious, non-hazardous research specimens in a dry shipper\n   * **IATA shipper's declaration** for magnetized material (dry shipper contains no free liquid but is classified as a cryogenic vessel)\n\n### 7. Shipment\n\n1. Schedule **FedEx International Priority** pickup from Jackson Laboratory (Bar Harbor, ME, USA).\n2. Ship **Monday\u2013Wednesday** to avoid weekend delays at customs.\n3. Estimated transit time: **3\u20135 business days** (Bar Harbor \u2192 Australian destination).\n4. Provide the receiving lab with the **tracking number** and expected arrival date.\n5. Alert the receiving lab to arrange customs clearance and immediate collection upon arrival.\n\n### 8. Receipt at Australian Laboratory\n\n1. **Collect the shipment immediately** upon arrival \u2014 do not allow it to sit at the depot.\n2. Open the dry shipper and verify:\n   * All cryovials are present and match the packing list\n   * Vials are still frozen (frost visible on vials)\n   * No signs of thawing (condensation, liquid in vials)\n   * Temperature data logger reading (if included) \u2014 confirm temperature remained below \u2212150 \u00b0C throughout transit\n3. Transfer all cryovials immediately to a **\u221280 \u00b0C freezer**.\n4. Log receipt in the laboratory sample inventory:\n\n   | Field | Record |\n   |---|---|\n   | Date received | |\n   | Shipment tracking # | |\n   | Dry shipper condition | Frost present / temperature OK |\n   | Number of vials received | |\n   | Vials match packing list? | Yes / No (note discrepancies) |\n   | Storage location | Freezer ID, shelf, box, position |\n   | Received by | Name and signature |\n\n5. Return the **dry shipper** to Jackson Laboratory per agreed logistics (typically FedEx return shipment).\n\n---\n\n## Quality Control\n\n* **RNA integrity:** Downstream RNA extraction should target RIN/RQN \u2265 7.0 for all tissues. Pituitary may yield lower due to small size \u2014 RQN \u2265 5.0 acceptable.\n* **Dissection time:** Total time from euthanasia to snap-freeze should be **<15 minutes per animal**. Record actual times.\n* **Freeze-thaw:** Tissues must not undergo any freeze-thaw cycles before processing. Monitor freezer temperature logs.\n\n## Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Pituitary fragmented or lost | Record in log; may need to pool from multiple animals or exclude |\n| Tissue partially thawed on arrival | Record in log; process immediately for extraction; note in downstream QC |\n| Customs delay >48 h | Contact FedEx and customs broker; verify dry shipper hold time is sufficient |\n| Dry shipper running low | If hold time is marginal, arrange emergency transfer to local \u221280 \u00b0C |\n| Import permit expired | Do not ship; renew permit before proceeding (allow 4\u20136 weeks) |\n| Animal health abnormality | Record observation; PI to decide whether to include or exclude the animal |\n\n## Waste Disposal\n\n* Mouse carcasses: dispose per Jackson Laboratory IACUC-approved procedures (incineration or licensed biological waste contractor).\n* Sharps (scalpel blades, needles): sharps container.\n* Liquid nitrogen: allow to evaporate in well-ventilated area. Do not pour down drains.\n\n## Records to Maintain\n\n* Approved IACUC protocol number\n* Australian import permit (DAFF) \u2014 copy\n* Dissection logs for each animal\n* Shipping documentation (commercial invoice, packing list, tracking number)\n* Temperature logger data (if used)\n* Sample receipt log\n* Freezer storage map / inventory database entry\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-40",
            "@type": "LabProtocol",
            "name": "Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit",
            "description": "Simultaneous purification of genomic DNA and total RNA from the same tissue sample (\u226430 mg) using selective AllPrep DNA spin columns and RNeasy silica membrane technology. Includes on-column DNase treatment for RNA.",
            "url": {
                "@id": "https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/multianalyte-and-virus/allprep-dna-rna-mini-kit"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "# SOP: Simultaneous DNA and RNA Extraction from Animal Tissues using QIAGEN AllPrep DNA/RNA Mini Kit\n\n**Applies to:** Fresh or frozen animal tissues \u226430 mg per spin column\n**Kit:** QIAGEN AllPrep DNA/RNA Mini Kit (Cat. No. 80204)\n**Location:** __________\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nSimultaneous purification of genomic DNA and total RNA from the same tissue sample using the AllPrep DNA spin column (selective DNA binding in high-salt buffer) and the RNeasy spin column (silica membrane RNA binding). This enables paired DNA/RNA analysis from a single biopsy or tissue aliquot.\n\n## Scope\n\nAll personnel performing dual DNA/RNA extraction from animal tissues in this laboratory.\n\n## Responsibilities\n\n* **Operators:** Follow this SOP, record batch details, and maintain RNase-free practice.\n* **Supervisors:** Ensure training, PPE compliance, and kit maintenance.\n\n## Safety and RNase-Free Practice\n\n* Wear lab coat, gloves, and eye protection.\n* Work in a clean area. Treat benches and tools with RNase decontamination solution.\n* \u03b2-mercaptoethanol is toxic and has a strong odor. Handle in a fume hood.\n* Use RNase-free tubes, tips, and reagents only. Change gloves frequently.\n* Buffer RLT Plus contains guanidine isothiocyanate \u2014 harmful if swallowed or in contact with skin. Do not mix with bleach.\n\n## References\n\n* QIAGEN AllPrep DNA/RNA Mini Handbook, November 2020.\n* NASA GeneLab SOP GL-SOP-3.1 v1.0.\n\n---\n\n## Materials and Equipment\n\n**Provided in kit (Cat. No. 80204, 50 preps)**\n\n* Buffer RLT Plus\n* Buffer RW1\n* Buffer RPE (concentrate \u2014 add ethanol before first use)\n* Buffer AW1\n* Buffer AW2\n* AllPrep DNA Mini Spin Columns\n* RNeasy Mini Spin Columns\n* Collection Tubes (1.5 mL and 2 mL)\n* RNase-free Water\n\n**Not supplied**\n\n* \u03b2-mercaptoethanol (\u03b2-ME)\n* 96\u2013100% ethanol\n* 70% ethanol (freshly prepared)\n* RNase-free DNase Set (Cat. No. 79254) \u2014 for on-column DNase treatment\n* RNase/DNase-free 1.5 mL tubes\n* Microcentrifuge capable of \u226512,000 \u00d7 g\n* Homogenization tools: rotor-stator homogenizer, or TissueLyser with stainless steel beads (3\u20137 mm), or RNase-free pestle, or mortar and pestle with liquid nitrogen\n* Optional: RNase-free syringe with 20-gauge needle\n\n---\n\n## Preparations (before each extraction session)\n\n1. **Prepare Buffer RPE** (first use only)\n   * Add **4 volumes of 96\u2013100% ethanol** to the concentrate. Mark the bottle to indicate ethanol has been added.\n\n2. **Prepare fresh Lysis Buffer**\n   * Add **10 \u00b5L \u03b2-mercaptoethanol per 1 mL Buffer RLT Plus**. Mix well. Prepare only what you need; discard unused buffer.\n\n3. **Prepare DNase Mix** (per sample)\n   * Dissolve lyophilized DNase I (1500 Kunitz units) in **550 \u00b5L** RNase-free water. Mix gently. Aliquot and store at \u221220 \u00b0C (stable up to 9 months; thawed aliquots stable at 2\u20138 \u00b0C for 6 weeks).\n   * Per sample: **10 \u00b5L DNase I stock + 70 \u00b5L Buffer RDD**. Mix by gentle inversion \u2014 **do not vortex**.\n\n4. **Pre-chill microcentrifuge** to 4 \u00b0C if required for lysate clarification.\n\n5. **Heat 2 mL RNase/DNase-free water** to 70 \u00b0C for DNA elution (optional, improves yield).\n\n6. **Input tissue mass**\n\n   | Tissue type | Maximum input |\n   |---|---|\n   | Standard soft tissue | \u226430 mg |\n   | Muscle, heart, skin | \u226415 mg (LN\u2082-preserved) or \u226410 mg (RNAlater) |\n   | Liver | \u226420 mg |\n\n---\n\n## Procedure\n\n### 1. Tissue Disruption and Homogenization\n\n1. Weigh tissue (\u226430 mg). Place in a pre-chilled RNase-free tube on ice.\n2. Add **600 \u00b5L Buffer RLT Plus** (with \u03b2-ME).\n3. Homogenize immediately using one of the following:\n\n   **Option A \u2014 Rotor-stator:**\n   Homogenize for **30\u201345 s** until no visible tissue pieces remain.\n\n   **Option B \u2014 TissueLyser / bead mill:**\n   Add a single **5 mm stainless steel bead**. Run at **25 Hz for 2 \u00d7 2 min** (rotate adapter halfway).\n\n   **Option C \u2014 Mortar & pestle with liquid nitrogen:**\n   Grind tissue to a fine powder under LN\u2082. Transfer powder to a tube, let LN\u2082 evaporate, then add Buffer RLT Plus and vortex.\n\n   **Option D \u2014 Needle and syringe:**\n   Pass lysate through a **20-gauge needle** at least **5\u201310 times** until homogeneous.\n\n4. Centrifuge the lysate at **full speed (\u226512,000 \u00d7 g) for 3 min**, RT.\n5. Carefully transfer the supernatant to a new tube. **Do not disturb the pellet.**\n\n### 2. Bind Genomic DNA\n\n1. Transfer the cleared lysate onto an **AllPrep DNA Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 30 s**, RT.\n3. **Set aside the DNA column** at 4 \u00b0C (or RT for up to 1 h) \u2014 proceed to RNA purification first.\n4. **Save the flow-through** for RNA isolation.\n\n### 3. Adjust RNA Binding Conditions\n\n1. Add **1 volume of freshly prepared 70% ethanol** to the flow-through (e.g. 600 \u00b5L ethanol to 600 \u00b5L flow-through). For **liver tissue**, use **50% ethanol** instead.\n2. Mix well by pipetting. **Do not centrifuge.** A precipitate may form \u2014 this is normal; proceed immediately.\n\n### 4. Bind and Wash RNA\n\n1. Transfer up to **700 \u00b5L** of the sample onto an **RNeasy Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Repeat until all sample has passed through the column.\n4. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 5. On-Column DNase Treatment\n\n1. Pipette **80 \u00b5L DNase Mix** (10 \u00b5L DNase I + 70 \u00b5L Buffer RDD) directly onto the membrane.\n2. Incubate at **RT for 15\u201330 min** (30 min recommended for maximum removal).\n3. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 6. Wash and Dry RNA Column\n\n1. **Replace the collection tube** with a new 2 mL tube.\n2. Add **500 \u00b5L Buffer RPE** (ethanol added). Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add another **500 \u00b5L Buffer RPE**. Centrifuge at **\u22658,000 \u00d7 g for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 7. Elute RNA\n\n1. Place the RNeasy column in a new **1.5 mL collection tube**.\n2. Add **30\u201350 \u00b5L RNase-free water** directly onto the membrane.\n3. Incubate **1\u20135 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** RNase-free water for maximum yield. Pool eluates if the second elution is \u226530% of the first.\n6. Place RNA on ice immediately.\n\n### 8. Wash Genomic DNA Column\n\n1. Retrieve the AllPrep DNA column from step 2.\n2. Add **500 \u00b5L Buffer AW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add **500 \u00b5L Buffer AW2**. Centrifuge at **full speed (\u226514,000 \u00d7 g) for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 9. Elute Genomic DNA\n\n1. Place the AllPrep DNA column in a new **1.5 mL collection tube**.\n2. Add **50\u2013100 \u00b5L RNase/DNase-free water** (pre-warmed to 70 \u00b0C for improved yield) directly onto the membrane.\n3. Incubate **2 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** for maximum yield.\n\n### 10. Quantification and Quality Assessment\n\n**RNA:**\n* Measure concentration by Qubit RNA assay or NanoDrop A260.\n* Record A260/280 (expect \u22651.8) and A260/230 (expect \u22651.5) ratios.\n* Assess integrity by Fragment Analyzer, Bioanalyzer, or TapeStation (target RQN/RIN \u22657 for most applications).\n\n**DNA:**\n* Measure concentration by Qubit dsDNA assay or NanoDrop A260.\n* Record A260/280 (expect ~1.8) and A260/230 ratios.\n* Assess fragment size by TapeStation Genomic DNA assay or agarose gel if required.\n\n### 11. Storage\n\n* **RNA:** Keep on ice for immediate use. Store at **\u221280 \u00b0C** for long term.\n* **DNA:** Store at **\u221220 \u00b0C** (short term) or **\u221280 \u00b0C** (long term). Avoid repeated freeze-thaw cycles.\n\n---\n\n## Special Considerations for Muscle, Heart, and Skin Tissues\n\nThese tissues are dense and fibrous, leading to potential column clogging and lower yields.\n\n* Start with **\u226415 mg** (LN\u2082-preserved) or **\u226410 mg** (RNAlater-preserved).\n* **Pre-warm** Buffers RW1 and RPE to **37 \u00b0C** for 30 min before use.\n* Centrifuge lysate **twice** at full speed to minimize cell debris.\n* Load a maximum of **400 \u00b5L** per column pass.\n* If the column clogs: incubate wash solution on column for **5 min at 37 \u00b0C** before centrifuging.\n* Elute with **pre-warmed (37 \u00b0C) water** in two passes (50 \u00b5L + 30 \u00b5L); combine if second elution is \u226530% of first.\n* If yields remain low: process **two 15 mg aliquots separately** and combine eluates.\n\n---\n\n## Acceptance and Rejection Criteria\n\n* Elution volume and expected yield range documented per tissue type.\n* No visible carryover of debris in eluate.\n* Proceed if spectrophotometric and integrity checks meet project-specific thresholds.\n\n## Expected Yields\n\n| Tissue type | DNA yield | RNA yield |\n|---|---|---|\n| Liver (20 mg) | 10\u201330 \u00b5g | 20\u201360 \u00b5g |\n| Brain (20 mg) | 5\u201315 \u00b5g | 5\u201320 \u00b5g |\n| Kidney (20 mg) | 5\u201315 \u00b5g | 10\u201340 \u00b5g |\n| Heart/Muscle (15 mg) | 2\u201310 \u00b5g | 1\u201310 \u00b5g |\n| Spleen (20 mg) | 15\u201340 \u00b5g | 15\u201360 \u00b5g |\n\n## Troubleshooting\n\n| Problem | Possible cause | Solution |\n|---|---|---|\n| Low RNA yield | Incomplete lysis | Increase homogenization time; ensure \u03b2-ME was added to RLT Plus |\n| Low RNA yield | Column overloaded | Reduce input tissue mass |\n| Low DNA yield | Insufficient lysis | Ensure thorough mechanical disruption before loading AllPrep DNA column |\n| Low DNA yield | DNA column dried out | Do not leave DNA column at RT for >1 h |\n| Genomic DNA in RNA | DNase treatment insufficient | Extend incubation to 30 min; verify DNase I activity |\n| RNA in DNA | Carryover of flow-through | Avoid disturbing interface when transferring lysate |\n| Column clogging | Tissue debris | Pre-clear lysate by additional centrifugation; reduce input mass |\n| Low A260/230 | Guanidine salt carryover | Add an extra RPE/AW2 wash step |\n\n## Waste Disposal\n\n* Collect \u03b2-mercaptoethanol and guanidine-containing wastes according to institutional chemical waste procedures.\n* Dispose of biological materials under local biosafety rules.\n* Spin columns with bound nucleic acids are non-hazardous after elution.\n\n## Records to Maintain\n\n* Date, operator, sample ID, tissue type and mass, homogenization method, reagent lot numbers, elution volume(s), DNA and RNA concentrations, purity ratios, integrity assessment, and storage location.\n* Deviations and corrective actions.\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-37",
            "@type": "LabProtocol",
            "name": "PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)",
            "description": "Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library preparation from total RNA. Uses template-switching reverse transcription followed by PCR amplification with barcoded primers. Compatible with MinION, GridION and PromethION flow cells (R9.4.1). Superseded by SQK-PCS114 in late 2023.",
            "url": {
                "@id": "https://community.nanoporetech.com/docs/prepare/library_prep_protocols/pcr-cdna-barcoding-kit-sqk-pcb111-24"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "## Overview & Kit Contents\n\nThis protocol is based on the Oxford Nanopore Technologies **PCR-cDNA Barcoding Kit (SQK-PCB111.24)**, suitable for generating full-length barcoded cDNA sequencing libraries from total RNA.\n\n### Kit components\n- VNP primer, SSP primer (strand-switching primer)\n- Barcoded PCR primers (BC01\u2013BC24)\n- Rapid Adapter (RAP)\n- Elution Buffer (EB)\n\n### Required reagents (user-supplied)\n- Lambda Exonuclease\n- NEBNext Quick Ligation Reaction Buffer\n- T4 DNA Ligase 2M U/ml\n- 10 mM dNTP solution\n- LongAmp Hot Start Taq 2X Master Mix\n- Maxima H Minus Reverse Transcriptase (200 U/ul) with 5x RT Buffer\n- RNaseOUT, 40 U/ul\n- USER (Uracil-Specific Excision Reagent) Enzyme\n- Exonuclease I\n- AMPure XP beads\n\n### Required equipment\n- Thermal cycler, magnetic rack, Qubit fluorometer, vortex mixer, microcentrifuge"
                },
                {
                    "@type": "HowToStep",
                    "position": 2,
                    "text": "### Input RNA requirements\n\n- **Input**: 1\u201350 ng poly(A)+ mRNA or 50\u2013100 ng total RNA (RIN >= 7 recommended)\n- RNA should be DNase-treated if genomic DNA contamination is suspected\n- Prepare RNA in 7.5 uL nuclease-free water\n- Add 1 uL VNP primer (2 uM) and 1 uL 10 mM dNTPs\n- Incubate at **65 C for 5 min**, then snap-cool on ice for 1 min"
                },
                {
                    "@type": "HowToStep",
                    "position": 3,
                    "text": "### Reverse transcription and strand switching (~90 min)\n\n1. Prepare RT mix on ice (per reaction):\n   - 4 uL 5x RT Buffer\n   - 1 uL RNaseOUT (40 U/uL)\n   - 1 uL nuclease-free water\n   - 2 uL Strand-Switching Primer (SSP, 10 uM)\n   - 1 uL Maxima H Minus RT (200 U/uL)\n2. Add 10 uL RT mix to the 9.5 uL RNA/primer/dNTP mix (total 19.5 uL)\n3. Incubate: **42 C for 90 min** (RT + template switching), **85 C for 5 min** (inactivation), hold 4 C\n4. Add 1 uL RNase H, incubate **37 C for 10 min**\n5. Briefly centrifuge and place on ice"
                },
                {
                    "@type": "HowToStep",
                    "position": 4,
                    "text": "### PCR amplification with barcoded primers (~30 min)\n\n1. Prepare PCR reaction (50 uL total):\n   - 20 uL cDNA from RT step\n   - 25 uL LongAmp Taq 2X Master Mix\n   - 1.5 uL Barcode Primer (BC*XX*, 10 uM)\n   - 3.5 uL nuclease-free water\n2. Cycling conditions:\n   - **95 C, 3 min** (initial denaturation)\n   - **18 cycles**: 95 C 15 s / 62 C 15 s / 65 C 5 min\n   - **65 C, 6 min** (final extension)\n   - Hold at 4 C\n\n**Note**: 18 cycles is suitable for 50-100 ng total RNA input. Reduce to 14-15 cycles for higher inputs."
                },
                {
                    "@type": "HowToStep",
                    "position": 5,
                    "text": "### Bead clean-up and quantification\n\n1. Add 40 uL AMPure XP beads (0.8x ratio) to 50 uL PCR product\n2. Incubate at RT for 5 min\n3. Place on magnetic rack, wait for pellet to form\n4. Wash 2x with 200 uL freshly prepared 80% ethanol\n5. Air-dry pellet for ~30 s (do not over-dry)\n6. Elute in 12 uL EB\n7. Quantify with Qubit dsDNA HS assay\n8. Expected yield: 50-500 ng depending on input RNA quality and quantity"
                },
                {
                    "@type": "HowToStep",
                    "position": 6,
                    "text": "### Pool barcoded libraries and attach Rapid Adapter\n\n1. Pool equimolar amounts of barcoded cDNA libraries (target: 100-200 fmol total)\n2. Adjust volume to 11 uL with EB\n3. Add 1 uL Rapid Adapter (RAP)\n4. Mix gently by flicking, spin down briefly\n5. Incubate at **RT for 5 min**\n6. Library is ready for loading -- **do not vortex** after adapter attachment"
                },
                {
                    "@type": "HowToStep",
                    "position": 7,
                    "text": "### Flow cell priming and loading\n\n1. Prepare priming mix: 30 uL FCT + 1170 uL FCF\n2. Load 800 uL priming mix via priming port, wait 5 min\n3. Prepare loading mix: 12 uL adapted library + 37.5 uL SB + 25.5 uL LB\n4. Load remaining priming mix, then load 75 uL library via SpotON port\n5. Start sequencing in MinKNOW (Kit: SQK-PCB111.24, enable barcode demultiplexing)"
                },
                {
                    "@type": "HowToStep",
                    "position": 8,
                    "text": "## References\n\n- Oxford Nanopore Technologies, **\"PCR-cDNA Barcoding Kit (SQK-PCB111.24)\"**.\n- Earlier version: SQK-PCS111. Kit superseded by SQK-PCS114/SQK-PCB114 (V14 chemistry, R10.4.1 flow cells) in late 2023."
                }
            ]
        },
        {
            "@id": "#protocol-44",
            "@type": "LabProtocol",
            "name": "Fragment Analyzer \u2013 SS NGS Fragment 1-6000 bp",
            "description": "Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-473 kit. Suitable for QC of NGS libraries, PCR products, and size-selected DNA fragments in the 1-6000 bp range.",
            "url": {
                "@id": "https://www.agilent.com/store/en_US/Prod-DNF-473-0500/DNF-473-0500"
            },
            "version": "1.0",
            "category": "measurement",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "**Method:** `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds`\n\n**Instrument:** Agilent/Advanced Analytical Fragment Analyzer (33 cm capillary array)\n\n**Analysis Mode:** NGS\n\n---\n\n## 1. Document Control\n\n* **SOP ID:** FA-NGS-1-6000bp-33cm\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Prepared by:** __________\n* **Approved by:** __________\n* **Review cycle:** 12 months\n\n## 2. Purpose\n\nTo define the procedure for running standard-sensitivity NGS fragment analysis on the Fragment Analyzer using the **DNF-473-33** method. This method resolves DNA fragments in the **1\u20136,000 bp** range with standard sensitivity, suitable for:\n\n* QC of NGS libraries (amplicon, PCR-cDNA, shotgun)\n* Assessment of size selection efficiency (e.g. AMPure bead selection)\n* Verification of library size distribution prior to sequencing\n* Quantification of library concentration\n\n## 3. Scope\n\nApplies to all operators performing NGS library QC or fragment analysis in the 1\u20136,000 bp range using the DNF-473 kit on a Fragment Analyzer.\n\n## 4. Responsibilities\n\n* **Operator:** Execute this SOP, record run metadata, verify QC, and report issues.\n* **Lab lead:** Ensure training, instrument maintenance, and SOP review.\n\n## 5. Safety\n\n* Wear lab coat, safety glasses, and disposable gloves.\n* Handle gels, buffers, and plates per SDS.\n* Avoid contact with high voltage areas during operation. Only open instrument when software indicates safe.\n* Dispose of consumables as per local regulations.\n\n## 6. References\n\n* Fragment Analyzer user manual.\n* DNF-473 SS NGS Fragment 1-6000bp kit instructions.\n* Local laboratory policies for data retention and equipment maintenance.\n\n## 7. Materials and Equipment\n\n**Instrument and configuration**\n\n* Fragment Analyzer with **33 cm** capillary array.\n* Array serial and usage count recorded before each run.\n\n**Reagents and consumables**\n\n* SS NGS Fragment 1-6000bp gel cartridge compatible with `DNF-473-33`.\n* Buffer reservoirs and rinse solution as per kit.\n* 96-well plate(s) and plate seals or caps.\n* DNA ladder (supplied with kit or compatible sizing standard).\n* Nuclease-free water and low-retention tips.\n\n**Software**\n\n* ProSize 3.0 or later for data analysis and PDF report generation.\n\n## 8. Sample Requirements\n\n* DNA libraries in TE, EB, or nuclease-free water.\n* Recommended input: **0.2\u201350 ng/\u00b5L** (standard sensitivity range).\n* Minimum volume per well: as per plate format (typically 2 \u00b5L sample + diluent).\n* Avoid EDTA >5 mM, high salt, or detergents that interfere with electrokinetic injection.\n* For very low concentration libraries (<1 ng/\u00b5L, e.g. post-bead-selection), results may show concentration but no resolved peaks \u2014 this is expected and the TIM value is still informative.\n\n## 9. Pre-Run Checks\n\n1. Inspect capillary array for leaks, kinks, or salt crystallisation. Record **array serial** and **usage count**.\n2. Confirm instrument details in software:\n   * **Device Serial:** record\n   * **FA Version:** record\n3. Verify consumables:\n   * Correct **gel** loaded and **Gel Selection: Gel 1**.\n   * Fresh buffer and rinse trays in correct positions.\n4. Equilibrate reagents to room temperature if required by kit.\n5. Prepare plate map with samples, ladder, controls, and blanks.\n\n## 10. Plate Preparation\n\n1. Thaw and mix reagents gently. Spin down briefly.\n2. Aliquot ladder into designated well (typically position 12 of each row).\n3. Aliquot samples into designated wells per plate map.\n4. Include at least one blank (nuclease-free water) for baseline reference.\n5. Seal the plate. Briefly centrifuge to remove bubbles. Keep sealed until loading.\n\n## 11. Instrument Setup (Software)\n\nSet parameters as follows:\n\n| Parameter | Setting |\n|---|---|\n| Method Name | `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds` |\n| Analysis Mode | `NGS` |\n| Gel Prime | `No` |\n| Full Conditioning | `Yes` (start of day) |\n| Gel Selection | `Gel 1` |\n| Perform Prerun | `5.0 kV` for `30 s` |\n| Rinse | Tray `3`, Row `A`, `# Dips: 2` |\n| Marker 1 | `No` |\n| Sample Injection | `5.0 kV` for `4 s` |\n| Separation | `8.0 kV` for `40.0 min` |\n| # of Capillaries | Per array configuration |\n\n**Note:** The DNF-473 method uses **shorter separation time (40 min)** and **higher separation voltage (8 kV)** compared to the HS Large Fragment method (DNF-464: 60 min, 5 kV), reflecting the smaller target size range.\n\n## 12. Run Procedure\n\n1. Load the sealed sample plate into the instrument.\n2. Confirm tray positions and liquid levels.\n3. In software, confirm the method parameters match Section 11.\n4. Start the run. Observe for the first 2\u20133 minutes for abnormal current, leaks, or errors.\n5. Allow the **40 min** separation to complete.\n\n## 13. Data Analysis (ProSize)\n\n1. Open the completed run in ProSize 3.0.\n2. Verify ladder sizing is correct (expected peaks at kit-specified positions).\n3. For each sample, review:\n   * **Electropherogram** \u2014 peak shape, baseline, and resolution\n   * **Total Concentration (ng/\u00b5L)** \u2014 integrated from all non-marker peaks\n   * **TIM (nmol/L)** \u2014 total integrated molarity\n   * **Main peak size (bp)** \u2014 dominant fragment size\n   * **Number of peaks** \u2014 indicator of library complexity/purity\n4. Export PDF report containing per-sample peak tables, electropherograms, and summary metrics.\n\n## 14. Interpretation Guide\n\n| Observation | Interpretation | Action |\n|---|---|---|\n| Single clean peak at expected size | Library prep and/or size selection successful | Proceed to pooling/sequencing |\n| Broad peak or multiple peaks | Heterogeneous library or incomplete size selection | Consider re-selection or accept depending on application |\n| No peaks but measurable concentration | Library below detection threshold for sizing | Verify by Qubit; may still be usable if concentration is sufficient |\n| Adapter dimer peak (~100-150 bp) | Incomplete cleanup | Re-clean with AMPure beads at appropriate ratio |\n| No signal | Failed library prep, sample degraded, or loading error | Troubleshoot: check sample, re-run, or re-prep |\n\n### Typical Results for ONT PCR-cDNA Libraries\n\n| Library state | Expected concentration | Expected main peak |\n|---|---|---|\n| Pre-size-selection | 4\u201310 ng/\u00b5L | 1,000\u20132,000 bp (broad) |\n| Post-bead-selection (1.5 kb) | 0.5\u20132 ng/\u00b5L | ~1,480 bp (single peak) |\n| Post-bead-selection (4 kb) | 0.5\u20132 ng/\u00b5L | ~3,500\u20134,500 bp (single peak) |\n\n## 15. Quality Control and Acceptance\n\n* Ladder wells exhibit expected profile and sizing.\n* Baseline is stable with distinct peaks where applicable.\n* Migration time consistent across capillaries.\n* If any criterion fails, document, recondition, and repeat controls before analysing unknowns.\n\n## 16. Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Weak or absent signal | Verify injection settings, sample concentration, plate seal integrity, capillary tips in wells |\n| High noise or drifting baseline | Re-condition array, check buffer freshness and tray cleanliness |\n| Inconsistent sizing | Confirm ladder integrity, plate map accuracy, and temperature stability |\n| Frequent current faults | Inspect for bubbles, low liquid levels, or salt contamination; replace consumables |\n\n## 17. Maintenance\n\n* Perform **Full Conditioning** at the start of the day or as required by kit.\n* Replace gels and buffers per shelf-life and use counts.\n* Track **Array Usage Count** at each run and follow vendor guidance for replacement.\n* Clean trays weekly or after spills. Inspect electrodes and stage area.\n\n## 18. Records to Retain\n\nFor each run record:\n\n* Date, time, and operator\n* Device serial and FA software version\n* Array serial and usage count\n* Method name and key parameters\n* Plate map and sample IDs\n* File path to raw data and PDF reports\n* QC outcomes and any deviations\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-35",
            "@type": "LabProtocol",
            "name": "Sequencing Submission",
            "description": "Submit samples for sequencing at the genomics facility. Links kevlab samples to QC app runs after sequencing is complete.",
            "version": "1.0",
            "category": "sequencing"
        },
        {
            "@id": "#measurement-344",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1493",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-345",
            "@type": "PropertyValue",
            "name": "count",
            "value": "3",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-342",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "5.6008",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
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