ONT Isoform Functional Annotation (superseded)

Barcode 22 functional annotation

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Type

CWL

Status

succeeded

Engine

cwltool

Duration

1.6 h

Source Data

Study	Strain-specific cortex gene expression and isoform usage
Sample prep	PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) 2023-05-09
Sequencing	Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) 2023-05-12
Run Data	Run #63 (6 samples)
Samples	C57_rep1_bc10 C57_rep2_bc11 C57_rep3_bc12 DBA_rep1_bc13 DBA_rep2_bc14 DBA_rep3_bc15

Pipeline

ONT Long-Read Transcriptomics (IsoQuant)

Run #44

succeeded

ONT Isoform Functional Annotation (superseded)

Run #51 (this run)

succeeded 1 sources

Combined From

#44 — ONT Long-Read Transcriptomics (IsoQuant) succeeded

Sample Provenance

Process Chain

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

8 samples produced

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

24 samples produced

Simultaneous extraction of DNA and RNA from C57 and DBA cortex tissue using Qiagen AllPrep (2016-11-17) Labbook

Protocol: Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit

16 samples produced

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24) (2023-03-13) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection) (2023-03-14) Labbook

24 measurements across 6 samples

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) (2023-05-09) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) (2023-05-12) Labbook

QC Measurements

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection)

2023-03-14 Labbook

Sample	bp	count	ng/µL	nmol/L
C57_rep1_bc19	1493	3	5.6008	6.1563
C57_rep2_bc20	1493	4	6.2061	7.4416
C57_rep3_bc21	1485	3	5.7241	6.8672
DBA_rep1_bc22	1500	2	4.2355	4.4695
DBA_rep2_bc23	1485	2	4.0452	4.2648
DBA_rep3_bc24	1485	2	4.3327	4.7107

Workflow

ONT Isoform Functional Annotation

#cwl

Software Tools

Tool	Version	URL
cwltool	-	https://github.com/common-workflow-language/cwltool
eldudy007_isoseq:1.0.sif	-	-

Results Summary

Unique Reads

510,986

from upstream transcriptomics

rRNA Reads

440 (0.1%)

mRNA Reads

510,546

used for CPM calculation

Transcripts Annotated

11,402

Pfam Hit Rate

93.8%

SwissProt Hit Rate

94.7%

High-Confidence

8,604 (75.5%)

Tier 1–2

NMD Candidates

315 (2.8%)

Evidence Tier Distribution

Novel Isoform Discovery

Total Isoforms

11,402

Reference

10,340

Novel (IsoQuant)

1,062

1,052 with ≥3 reads, 10 low-support

Novel %

9.3%

DV Genes: Novel-Created

307

Variation only from novel isoforms

DV Genes: Novel-Contributed

Novel isoforms add to existing ref variation

DV Genes: Reference-Only

654

Variation from reference annotation alone

Long-read sequencing discovered 1,062 novel isoforms (9.3%). Of 1,032 domain variant genes, 307 (30%) show domain architecture variation only because of novel isoforms not present in the reference annotation. In 8 DV genes, only novel isoforms produced detectable ORFs.

Isoform Read Support (CPM from upstream transcriptomics)

Novel Expression Rate

100.0%

1,062 / 1,062 (1,052 with ≥3 reads)

Reference Expression Rate

95.9%

9,915 / 10,340

Novel Median CPM

11.8

median 6 reads (mean 11.3, range 0–391)

Ref Median CPM

9.8

Novel (n=1,062)

Reference (n=10,340)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual isoform CPM values are overlaid as jittered points. Log₁₀ scale; dashed line marks CPM = 1 expression threshold. Per-model CPM from OUT.transcript_model_counts.tsv

Novel Isoform Functional Impact

Domain Altered

108

10.2% of novel

NMD Candidates

7.0% of novel

ORF Disrupted

134

12.6% of novel

Conserved

709

66.8% — same protein

1005 of 1062 novel isoforms (94.6%) have SwissProt protein homologs. 37 isoforms have no detectable protein homolog. Top species: Mus musculus (882), Homo sapiens (50), Bos taurus (38), Rattus norvegicus (26), Macaca fascicularis (3).

High-Impact Novel Isoforms (150)

Gene	Impact	Details	ORF	Reads	CPM	SwissProt
Chn1 `transcript1228.2.nic`	Domain altered	Lost: zf-RING_9	complete	139	272.3	sp\|P30337\|CHIN_RAT 99.6% — N-chimaerin
RBBP4 `transcript1494.4.nic`	Domain altered	Lost: Hexon	complete	53	103.8	sp\|Q3MHL3\|RBBP4_BOVIN 95.5% — Histone-binding protein RBBP4
YWHAE `transcript1796.11.nic`	Domain altered	Lost: Ta0938	complete	52	101.9	sp\|P62261\|1433E_BOVIN 100.0% — 14-3-3 protein epsilon
Il18 `transcript848.9.nic`	Domain altered	Gained: GT-A	complete	39	76.4	sp\|P70380\|IL18_MOUSE 100.0% — Interleukin-18
Nap1l1 `transcript1535.10.nnic`	Domain altered	Gained: SH3, TPR	complete	35	68.6	sp\|P28656\|NP1L1_MOUSE 99.4% — Nucleosome assembly protein 1-...
MYL12B `transcript2078.17.nnic`	Domain altered	Lost: DUF2267, DUF2666, DUF3741, GPHH, NADP_Rossmann, Trigger_C, UPF0154	complete	34	66.6	sp\|O14950\|ML12B_HUMAN 63.4% — Myosin regulatory light chain ...
Grb14 `transcript1113.2.nic`	Domain altered	Lost: BPS, PH, Ubiquitin	complete	33	64.6	sp\|Q9JLM9\|GRB14_MOUSE 88.3% — Growth factor receptor-bound p...
Lin7b `transcript1623.7.nic`	Domain altered	Gained: DUF2057	complete	26	50.9	sp\|O88951\|LIN7B_MOUSE 98.5% — Protein lin-7 homolog B
Ovol2 `transcript2604.2.nic`	Domain altered	Gained: Rubredoxin_2, YjdM_Zn_Ribbon	complete	25	49.0	sp\|Q8CIV7\|OVOL2_MOUSE 100.0% — Transcription factor Ovo-like ...
Bin1 `transcript234.18.nnic`	Domain altered	Lost: CK2S, DUF1664, Jnk-SapK_ap_N, OmpH, PTS_IIA, SNARE-fusion, TBD	complete	24	47.0	sp\|O08539\|BIN1_MOUSE 93.9% — Myc box-dependent-interacting ...
Rbck1 `transcript2779.2.nnic`	Domain altered	Lost: Rad50_zn_hook, Ubiquitin, zf-RanBP	complete	24	47.0	sp\|Q9WUB0\|HOIL1_MOUSE 97.7% — RanBP-type and C3HC4-type zinc...
Tpt1 `transcript1349.14.nnic`	Domain altered	Lost: DUF6613	complete	22	43.1	sp\|P63028\|TCTP_MOUSE 100.0% — Translationally-controlled tum...
Cd81 `transcript3714.7.nnic`	Domain altered	Lost: DUF6768	complete	21	41.1	sp\|P35762\|CD81_MOUSE 100.0% — CD81 antigen
Cdc123 `transcript27.2.nnic`	Domain altered	Lost: Beta_propeller	complete	19	37.2	sp\|Q8CII2\|CD123_MOUSE 87.5% — Translation initiation factor ...
Araf `transcript379.X.nnic`	Domain altered	Lost: PKinase	complete	19	37.2	sp\|P04627\|ARAF_MOUSE 100.0% — Serine/threonine-protein kinas...
Laptm4a `transcript63.12.nnic`	Domain altered	Lost: Circovir2_Orf4	complete	18	35.3	sp\|Q60961\|LAP4A_MOUSE 100.0% — Lysosomal-associated transmemb...
Khdrbs3 `transcript439.15.nnic`	Domain altered	Lost: FSA_C, STAR_dimer	complete	15	29.4	sp\|Q9R226\|KHDR3_MOUSE 100.0% — KH domain-containing, RNA-bind...
Psmb10 `transcript1844.8.nic`	Domain altered	Gained: 6PGD_C	complete	14	27.4	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Dctn6 `transcript310.8.nic`	Domain altered	Lost: Fucokinase	complete	14	27.4	sp\|Q9WUB4\|DCTN6_MOUSE 100.0% — Dynactin subunit 6
Sharpin `transcript622.15.nic`	Domain altered	Lost: Emfourin, PH	complete	13	25.5	sp\|Q91WA6\|SHRPN_MOUSE 100.0% — Sharpin
Pbx4 `transcript637.8.nic`	Domain altered	Lost: Carbpep_Y_N, DUF6779, EF_hand, HTH, Sec15, YdaS_antitoxin	complete	13	25.5	sp\|Q99NE9\|PBX4_MOUSE 36.5% — Pre-B-cell leukemia transcript...
Ubc `transcript1868.5.nnic`	Domain altered	Gained: DUF6393	complete	12	23.5	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Epn1 `transcript192.7.nnic`	Domain altered	Gained: DUF658	complete	12	23.5	sp\|Q80VP1\|EPN1_MOUSE 91.5% — Epsin-1
Gnptg `transcript476.17.nic`	Domain altered	Lost: AltA1	complete	12	23.5	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Zdhhc4 `transcript2513.5.nic`	Domain altered	Gained: DoxD-like	complete	11	21.6	sp\|Q9D6H5\|ZDHC4_MOUSE 100.0% — Palmitoyltransferase ZDHHC4
Tpd52l2 `transcript3745.2.nic`	Domain altered	Lost: DUF4164, Glyco_hydro_36, P-loop_NTPase, VBS-like	complete	11	21.6	sp\|Q9CYZ2\|TPD54_MOUSE 87.3% — Tumor protein D54
Mrps15 `transcript1283.4.nic`	Domain altered	Lost: GPS2_interact	complete	9	17.6	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Bax `transcript1642.7.nnic`	Domain altered	Lost: CF222	complete	9	17.6	sp\|Q07813\|BAX_MOUSE 100.0% — Apoptosis regulator BAX
Arl6ip1 `transcript2734.7.nnic`	Domain altered +NMD	Gained: Phage_holin_3_6	complete	9	17.6	sp\|Q9JKW0\|AR6P1_MOUSE 100.0% — ADP-ribosylation factor-like p...
Nsmce1 `transcript2903.7.nnic`	Domain altered	Gained: TMEMspv1-c74-12	complete	9	17.6	sp\|Q9D720\|NSE1_MOUSE 95.1% — Non-structural maintenance of ...
Bloc1s5 `transcript426.13.nnic`	Domain altered	Lost: Exonuc_VII_L, Stathmin, TPR	complete	9	17.6	sp\|Q8R015\|BL1S5_MOUSE 100.0% — Biogenesis of lysosome-related...
Kxd1 `transcript801.8.nnic`	Domain altered	Lost: TolA_bind_tri	complete	9	17.6	sp\|Q80XH1\|KXDL1_MOUSE 71.2% — KxDL motif-containing protein ...
Gap43 `transcript924.16.nnic`	Domain altered	Lost: EF_hand, GMC, Neuromodulin_N	complete	9	17.6	—
Cops4 `transcript1090.5.nic`	Domain altered	Lost: YabA	complete	8	15.7	sp\|O88544\|CSN4_MOUSE 100.0% — COP9 signalosome complex subun...
Sap30bp `transcript3284.11.nnic`	Domain altered	Gained: CCDC84	complete	8	15.7	sp\|Q02614\|S30BP_MOUSE 94.8% — SAP30-binding protein
Dab1 `transcript816.4.nic`	Domain altered	Gained: Globin	complete	8	15.7	sp\|Q8CJH2\|DAB1_RAT 99.0% — Disabled homolog 1
Dab1 `transcript817.4.nic`	Domain altered	Gained: Globin	complete	8	15.7	sp\|Q8CJH2\|DAB1_RAT 99.5% — Disabled homolog 1
Cct5 `transcript113.15.nic`	Domain altered	Lost: E-set	complete	7	13.7	sp\|P80316\|TCPE_MOUSE 100.0% — T-complex protein 1 subunit ep...
Smap `transcript2717.7.nnic`	Domain altered	Lost: BUD22, HAD, Nop14, Triple_barrel	complete	7	13.7	sp\|Q3MHL8\|SMAP_BOVIN 98.9% — Small acidic protein
Iqcb1 `transcript862.16.nic`	Domain altered	Lost: MLD	complete	7	13.7	sp\|Q8BP00\|IQCB1_MOUSE 100.0% — IQ calmodulin-binding motif-co...
Coq2 `transcript1095.5.nic`	Domain altered	Lost: DUF2798	complete	6	11.8	sp\|Q66JT7\|COQ2_MOUSE 100.0% — 4-hydroxybenzoate polyprenyltr...
Nap1l1 `transcript1531.10.nnic`	Domain altered	Gained: SH3, TPR	complete	6	11.8	sp\|P28656\|NP1L1_MOUSE 99.4% — Nucleosome assembly protein 1-...
Nap1l1 `transcript1539.10.nnic`	Domain altered	Gained: Nucleoplasmin	complete	6	11.8	sp\|P28656\|NP1L1_MOUSE 99.0% — Nucleosome assembly protein 1-...
Psmb10 `transcript1847.8.nic`	Domain altered	Gained: 6PGD_C	complete	6	11.8	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Lrrc28 `transcript1991.7.nic`	Domain altered	Lost: SPAN-X	complete	6	11.8	sp\|Q3TX51\|LRC28_MOUSE 100.0% — Leucine-rich repeat-containing...
ACTL6B `transcript2217.5.nic`	Domain altered	Lost: Rep	complete	6	11.8	sp\|A4FUX8\|ACL6B_BOVIN 100.0% — Actin-like protein 6B
Tsnax `transcript2330.8.nnic`	Domain altered	Lost: Phage_GPO	complete	6	11.8	sp\|Q9QZE7\|TSNAX_MOUSE 100.0% — Translin-associated protein X
Pagr1a `transcript3156.7.nnic`	Domain altered	Lost: Nop53, PAXIP1_C	complete	6	11.8	sp\|E9PUL5\|PRRT2_MOUSE 100.0% — Proline-rich transmembrane pro...
Araf `transcript391.X.nic`	Domain altered	Lost: PKinase	complete	6	11.8	sp\|P04627\|ARAF_MOUSE 100.0% — Serine/threonine-protein kinas...
Creb3 `transcript466.4.nnic`	Domain altered	Gained: GIT_CC	complete	6	11.8	sp\|Q61817\|CREB3_MOUSE 100.0% — Cyclic AMP-responsive element-...
Plekhj1 `transcript1042.10.nic`	Domain altered	Gained: Pinin_SDK_N	complete	5	9.8	sp\|Q9D240\|PKHJ1_MOUSE 92.7% — Pleckstrin homology domain-con...
Exosc1 `transcript1174.19.nnic`	Domain altered	Lost: Hybrid	complete	5	9.8	sp\|Q9DAA6\|EXOS1_MOUSE 97.8% — Exosome complex component CSL4
Gfra2 `transcript1278.14.nnic`	Domain altered	Gained: Apolipoprotein, HD-ZIP_N	complete	5	9.8	sp\|O08842\|GFRA2_MOUSE 99.5% — GDNF family receptor alpha-2
Htatip2 `transcript1816.7.nic`	Domain altered	Lost: Periplas_BP	complete	5	9.8	sp\|Q9Z2G9\|HTAI2_MOUSE 100.0% — Protein HTATIP2
Ubc `transcript1827.5.nnic`	Domain altered	Gained: DUF6393	complete	5	9.8	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Ubc `transcript1854.5.nnic`	Domain altered	Gained: DUF6393	complete	5	9.8	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Slc7a6os `transcript1861.8.nic`	Domain altered	Gained: Nop14	complete	5	9.8	sp\|Q7TPE5\|S7A6O_MOUSE 100.0% — Probable RNA polymerase II nuc...
Blvra `transcript2104.2.nnic`	Domain altered	Lost: NADP_Rossmann, SAM	complete	5	9.8	sp\|Q9CY64\|BIEA_MOUSE 100.0% — Biliverdin reductase A
Afg1l `transcript285.10.nnic`	Domain altered	Lost: DUF2075	complete	5	9.8	sp\|Q3V384\|AFG1L_MOUSE 100.0% — AFG1-like ATPase
Spock2 `transcript390.10.nnic`	Domain altered	Lost: Thyroglobulin_1	complete	5	9.8	sp\|Q9ER58\|TICN2_MOUSE 99.6% — Testican-2
CPLX2 `transcript618.13.nnic`	Domain altered +NMD	Lost: DUF2838	complete	5	9.8	sp\|P84088\|CPLX2_BOVIN 100.0% — Complexin-2
Iqcb1 `transcript864.16.nic`	Domain altered	Lost: MLD	complete	5	9.8	sp\|Q8BP00\|IQCB1_MOUSE 100.0% — IQ calmodulin-binding motif-co...
Tmem38a `transcript1085.8.nic`	Domain altered	Lost: LTXXQ-like	complete	4	7.8	sp\|Q3TMP8\|TM38A_MOUSE 100.0% — Trimeric intracellular cation ...
Hspb6 `transcript1146.7.nnic`	Domain altered	Lost: Crystallin	complete	4	7.8	sp\|Q5EBG6\|HSPB6_MOUSE 100.0% — Heat shock protein beta-6
Setd3 `transcript1269.12.nic`	Domain altered	Lost: OB_enterotoxin	complete	4	7.8	sp\|Q91WC0\|SETD3_MOUSE 100.0% — Actin-histidine N-methyltransf...
Mrps15 `transcript1282.4.nnic`	Domain altered	Lost: DUF272, DUF4140, DUF724, GPS2_interact, MIX23, PIN	complete	4	7.8	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Tspan31 `transcript1686.10.nnic`	Domain altered	Lost: Ac76, DUF6524, Herpes_LMP1, PHO4	complete	4	7.8	sp\|Q9CQ88\|TSN31_MOUSE 100.0% — Tetraspanin-31
Ndufs2 `transcript1799.1.nnic`	Domain altered	Lost: NiFeSe_Hases	complete	4	7.8	sp\|Q91WD5\|NDUS2_MOUSE 100.0% — NADH dehydrogenase [ubiquinone...
Snf8 `transcript2378.11.nic`	Domain altered	Lost: Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C, ZapD	complete	4	7.8	sp\|Q9CZ28\|SNF8_MOUSE 100.0% — Vacuolar-sorting protein SNF8
Abhd12 `transcript2708.2.nnic`	Domain altered	Gained: TRP_2	complete	4	7.8	sp\|Q99LR1\|ABD12_MOUSE 100.0% — Lysophosphatidylserine lipase ...
Aarsd1 `transcript2758.11.nic`	Domain altered	Lost: tRNA_synt_II	complete	4	7.8	sp\|Q3THG9\|AASD1_MOUSE 100.0% — Alanyl-tRNA editing protein Aa...
Cttn `transcript3754.7.nnic`	Domain altered	Lost: Histone	complete	4	7.8	sp\|Q60598\|SRC8_MOUSE 86.3% — Src substrate cortactin
Dap3 `transcript664.3.nic`	Domain altered	Lost: DUF4688	complete	4	7.8	sp\|Q9ER88\|RT29_MOUSE 100.0% — Small ribosomal subunit protei...
Cuta `transcript698.17.nic`	Domain altered +NMD	Lost: TIM_barrel	complete	4	7.8	sp\|Q9CQ89\|CUTA_MOUSE 93.2% — Protein CutA
Znf354b `transcript707.11.nnic`	Domain altered	Lost: BolA, C2H2-zf, RING, Ribosomal_L17, Tbcl_zf, XPA_N, Zn-ribbon_8, Zn_Beta_Ribbon, zinc_ribbon_15	complete	4	7.8	sp\|Q9QXT9\|Z354B_MOUSE 95.7% — Zinc finger protein 354B
Rnf181 `transcript713.6.nnic`	Domain altered	Lost: BIR-like, C2H2-zf, Rad50_zn_hook	complete	4	7.8	sp\|Q9CY62\|RN181_MOUSE 84.8% — E3 ubiquitin-protein ligase RN...
Rogdi `transcript74.16.nic`	Domain altered	Gained: Hemopexin	complete	4	7.8	sp\|Q3TDK6\|ROGDI_MOUSE 100.0% — Protein rogdi homolog
Ift74 `transcript743.4.nnic`	Domain altered	Gained: Crescentin	complete	4	7.8	sp\|Q8BKE9\|IFT74_MOUSE 96.7% — Intraflagellar transport prote...
Nol12 `transcript843.15.nic`	Domain altered	Lost: ALC	complete	4	7.8	sp\|Q8BG17\|NOL12_MOUSE 89.9% — Nucleolar protein 12
Sdr39u1 `transcript879.14.nic`	Domain altered	Lost: NADP_Rossmann	complete	4	7.8	sp\|Q5M8N4\|D39U1_MOUSE 100.0% — Epimerase family protein SDR39...
Sdr39u1 `transcript890.14.nic`	Domain altered	Lost: NADP_Rossmann	complete	4	7.8	sp\|Q5M8N4\|D39U1_MOUSE 100.0% — Epimerase family protein SDR39...
Pacrg `transcript96.17.nic`	Domain altered	Gained: Peptidase_PA, RNA_pol_Rpb2_2	complete	4	7.8	sp\|Q9DAK2\|PACRG_MOUSE 100.0% — Parkin coregulated gene protei...
Cers2 `transcript982.3.nic`	Domain altered	Lost: HTH, TRAM1, UPF0767	complete	4	7.8	sp\|Q924Z4\|CERS2_MOUSE 100.0% — Ceramide synthase 2
Fastkd3 `transcript984.13.nic`	Domain altered	Lost: FAST_2, TPR	complete	4	7.8	sp\|Q8BSN9\|FAKD3_MOUSE 100.0% — FAST kinase domain-containing ...
Aagab `transcript1273.9.nic`	Domain altered	Lost: FMN-binding	complete	3	5.9	sp\|Q8R2R3\|AAGAB_MOUSE 96.0% — Alpha- and gamma-adaptin-bindi...
KCTD1 `transcript132.18.nic`	Domain altered	Lost: DNA-mend, l-integrase_N	complete	3	5.9	sp\|Q719H9\|KCTD1_HUMAN 100.0% — BTB/POZ domain-containing prot...
Ankrd27 `transcript1320.7.nnic`	Domain altered	Lost: EcoEI_R_C, Ferritin, IDO-like, Sec8_exocyst	complete	3	5.9	sp\|Q3UMR0\|ANR27_MOUSE 100.0% — Ankyrin repeat domain-containi...
H2-Q10 `transcript1377.17.nic`	Domain altered	Lost: APC, Cadherin_C_2, Mucin	complete	3	5.9	sp\|P01898\|HA10_MOUSE 59.7% — H-2 class I histocompatibility...
Nme7 `transcript1667.1.nnic`	Domain altered	Lost: PH	complete	3	5.9	sp\|Q9QXL8\|NDK7_MOUSE 100.0% — Nucleoside diphosphate kinase ...
Acd `transcript1799.8.nnic`	Domain altered	Gained: OGFr_III	complete	3	5.9	sp\|Q5EE38\|ACD_MOUSE 100.0% — Adrenocortical dysplasia prote...
Me3 `transcript2185.7.nic`	Domain altered	Lost: NADP_Rossmann	complete	3	5.9	sp\|Q8BMF3\|MAON_MOUSE 100.0% — NADP-dependent malic enzyme, m...
Kcnk1 `transcript2358.8.nnic`	Domain altered	Lost: DUF6097	complete	3	5.9	sp\|O08581\|KCNK1_MOUSE 60.7% — Potassium channel subfamily K ...
Yipf2 `transcript241.9.nic`	Domain altered	Lost: CAT_RBD	complete	3	5.9	sp\|Q99LP8\|YIPF2_MOUSE 100.0% — Protein YIPF2
Mmd2 `transcript2437.5.nnic`	Domain altered	Gained: Ferlin_C	complete	3	5.9	sp\|Q8R189\|PAQRA_MOUSE 100.0% — Monocyte to macrophage differe...
C1qtnf12 `transcript2458.4.nic`	Domain altered	Lost: C1q_TNF	complete	3	5.9	sp\|Q8R2Z0\|ADIPL_MOUSE 100.0% — Adipolin
B3glct `transcript2680.5.nnic`	Domain altered	Lost: DUF3939	complete	3	5.9	sp\|Q8BHT6\|B3GLT_MOUSE 100.0% — Beta-1,3-glucosyltransferase
becn1 `transcript2745.11.nic`	Domain altered	Lost: CCDC-167, PP1c_bdg, Sec2p Gained: BCLiA, PI_PP_I	complete	3	5.9	sp\|O88597\|BECN1_MOUSE 100.0% — Beclin-1
Myl4 `transcript2981.11.nnic`	Domain altered	Gained: HTH	complete	3	5.9	sp\|P09541\|MYL4_MOUSE 100.0% — Myosin light chain 4
Phkg2 `transcript3211.7.nic`	Domain altered	Lost: FliG	complete	3	5.9	sp\|Q9DB30\|PHKG2_MOUSE 100.0% — Phosphorylase b kinase gamma c...
Rab40b `transcript3702.11.nnic`	Domain altered	Lost: P-loop_NTPase	complete	3	5.9	sp\|Q8VHP8\|RB40B_MOUSE 100.0% — Ras-related protein Rab-40B
Eif2a `transcript378.3.nnic`	Domain altered	Gained: DUF4407	complete	3	5.9	sp\|Q8BJW6\|EIF2A_MOUSE 100.0% — Eukaryotic translation initiat...
Strn4 `transcript379.7.nic`	Domain altered	Lost: CENP-H, Striatin	complete	3	5.9	sp\|P58404\|STRN4_MOUSE 100.0% — Striatin-4
Rps2 `transcript417.17.nic`	Domain altered +NMD	Lost: S5	complete	3	5.9	sp\|P25444\|RS2_MOUSE 100.0% — Small ribosomal subunit protei...
Ppp5c `transcript496.7.nnic`	Domain altered	Lost: AAA_lid, MIT	complete	3	5.9	sp\|Q60676\|PPP5_MOUSE 100.0% — Serine/threonine-protein phosp...
Rgs3 `transcript628.4.nic`	Domain altered	Lost: DUF4441	complete	3	5.9	sp\|Q9DC04\|RGS3_MOUSE 100.0% — Regulator of G-protein signali...
Tmem91 `transcript790.7.nnic`	Domain altered	Lost: AWPM-19	complete	3	5.9	sp\|Q8C581\|TMM91_MOUSE 100.0% — Transmembrane protein 91
Rtkn `transcript819.6.nic`	Domain altered	Lost: HR1	complete	3	5.9	sp\|Q8C6B2\|RTKN_MOUSE 100.0% — Rhotekin
Adrm1 `transcript866.3.nnic`	Domain altered	Lost: DUF1631	complete	3	5.9	sp\|Q9JKV1\|ADRM1_MOUSE 89.9% — Proteasomal ubiquitin receptor...
CALM1 `transcript1104.12.nnic`	ORF disrupted	Lost: AhpD-like, Cache, DUF2267, DUF3349, DUF5663, RTX_C, UBA Gained: LTXXQ-like	5prime_partial	205	401.5	sp\|P0DP23\|CALM1_HUMAN 100.0% — Calmodulin-1
Mbp `transcript1055.18.nnic`	ORF disrupted		5prime_partial	178	348.7	—
Nisch `transcript428.14.nic`	ORF disrupted		5prime_partial	61	119.5	sp\|Q80TM9\|NISCH_MOUSE 100.0% — Nischarin
Eno2 `transcript1541.6.nnic`	ORF disrupted	Lost: MBB, TIM_barrel	5prime_partial	59	115.6	sp\|P17183\|ENOG_MOUSE 94.4% — Gamma-enolase
Syp `transcript144.X.nnic`	ORF disrupted		5prime_partial	54	105.8	sp\|Q62277\|SYPH_MOUSE 100.0% — Synaptophysin
TUBB2A `transcript293.13.nnic`	ORF disrupted	Lost: Tubulin_C	5prime_partial	46	90.1	sp\|P09203\|TBB1_CHICK 99.4% — Tubulin beta-1 chain
Stoml2 `transcript430.4.nic`	ORF disrupted		5prime_partial	42	82.3	sp\|Q99JB2\|STML2_MOUSE 100.0% — Stomatin-like protein 2, mitoc...
Nptn `transcript1106.9.nic`	ORF disrupted		5prime_partial	39	76.4	sp\|P97546\|NPTN_RAT 98.7% — Neuroplastin
Ntan1 `transcript232.16.nic`	ORF disrupted		5prime_partial	36	70.5	sp\|Q64311\|NTAN1_MOUSE 100.0% — Protein N-terminal asparagine ...
Stx4 `transcript3264.7.nic`	ORF disrupted	Lost: COG2, Csm1_N, DUF3671, DUF3698, E-set, ETRAMP, His_Kinase_A, PRP1_N, T2SSF, TPR, TPR_MLP1_2, Vac_Fusion Gained: GOLD-like	3prime_partial	36	70.5	sp\|P70452\|STX4_MOUSE 100.0% — Syntaxin-4
P33monox `transcript643.13.nic`	ORF disrupted		5prime_partial	28	54.8	sp\|Q9DBN4\|P33MX_MOUSE 100.0% — Putative monooxygenase p33MONO...
HNRNPA2B1 `transcript515.6.nnic`	ORF disrupted	Lost: HnRNPA1, OB Gained: Concanavalin	5prime_partial	27	52.9	sp\|Q2HJ60\|ROA2_BOVIN 98.4% — Heterogeneous nuclear ribonucl...
Sparcl1 `transcript1171.5.nic`	ORF disrupted		3prime_partial	25	49.0	sp\|P70663\|SPRL1_MOUSE 100.0% — SPARC-like protein 1
Cryzl1 `transcript1256.16.nic`	ORF disrupted		5prime_partial	24	47.0	sp\|Q921W4\|QORL1_MOUSE 100.0% — Quinone oxidoreductase-like pr...
Napa `transcript324.7.nnic`	ORF disrupted		5prime_partial	22	43.1	sp\|Q9DB05\|SNAA_MOUSE 100.0% — Alpha-soluble NSF attachment p...
Pcsk1n `transcript229.X.nnic`	ORF disrupted	Lost: FUSC	5prime_partial	21	41.1	sp\|Q9QXV0\|PCS1N_MOUSE 99.5% — ProSAAS
Glrx3 `transcript3416.7.nic`	ORF disrupted	Lost: DUF4279, DUF953, GREB1_C, TrbC_Ftype Gained: Calycin, HeH, P-loop_NTPase, RdRP	5prime_partial	21	41.1	sp\|Q9CQM9\|GLRX3_MOUSE 100.0% — Glutaredoxin-3
Unc50 `transcript277.1.nic`	ORF disrupted		5prime_partial	20	39.2	sp\|Q9CQ61\|UNC50_MOUSE 100.0% — Protein unc-50 homolog
Araf `transcript381.X.nic`	ORF disrupted	Lost: PKinase	5prime_partial	18	35.3	sp\|P04627\|ARAF_MOUSE 100.0% — Serine/threonine-protein kinas...
Cuedc2 `transcript1339.19.nnic`	ORF disrupted		3prime_partial	17	33.3	sp\|Q9CXX9\|CUED2_MOUSE 91.6% — CUE domain-containing protein ...
Ccdc106 `transcript176.7.nic`	ORF disrupted	Gained: E-set	5prime_partial	17	33.3	sp\|Q3ULM0\|CC106_MOUSE 100.0% — Coiled-coil domain-containing ...
Tmem198 `transcript828.1.nic`	ORF disrupted		3prime_partial	17	33.3	sp\|Q8BG75\|TM198_MOUSE 100.0% — Transmembrane protein 198
Nme1 `transcript2290.11.nic`	ORF disrupted	Gained: DUF6486	5prime_partial	16	31.3	sp\|P15532\|NDKA_MOUSE 85.1% — Nucleoside diphosphate kinase ...
Tango2 `transcript452.16.nic`	ORF disrupted		5prime_partial	16	31.3	sp\|P54797\|TNG2_MOUSE 100.0% — Transport and Golgi organizati...
Septin4 `transcript2197.11.nic`	ORF disrupted		5prime_partial	15	29.4	sp\|P28661\|SEPT4_MOUSE 99.6% — Septin-4
Rtkn `transcript817.6.nic`	ORF disrupted		5prime_partial	15	29.4	sp\|Q8C6B2\|RTKN_MOUSE 95.5% — Rhotekin
Fam32a `transcript1059.8.nic`	ORF disrupted		5prime_partial	14	27.4	sp\|Q9CR80\|FA32A_MOUSE 100.0% — Protein FAM32A
Ephx4 `transcript1191.5.nic`	ORF disrupted		5prime_partial	14	27.4	sp\|Q6IE26\|EPHX4_MOUSE 99.0% — Epoxide hydrolase 4
Emc7 `transcript1742.2.nnic`	ORF disrupted		5prime_partial	14	27.4	sp\|Q9EP72\|EMC7_MOUSE 99.4% — Endoplasmic reticulum membrane...
Nat14 `transcript153.7.nnic`	ORF disrupted		5prime_partial	13	25.5	sp\|Q8BVG8\|NAT14_MOUSE 100.0% — Probable N-acetyltransferase 1...
Ntan1 `transcript236.16.nic`	ORF disrupted		5prime_partial	13	25.5	sp\|Q64311\|NTAN1_MOUSE 83.3% — Protein N-terminal asparagine ...
Urm1 `transcript612.2.nnic`	ORF disrupted		5prime_partial	13	25.5	sp\|Q9D2P4\|URM1_MOUSE 100.0% — Ubiquitin-related modifier 1
YPEL3 `transcript2990.7.nic`	ORF disrupted		5prime_partial	12	23.5	sp\|A6QPH8\|YPEL3_BOVIN 100.0% — Protein yippee-like 3
U2af1l4 `transcript1156.7.nic`	ORF disrupted		5prime_partial	11	21.6	sp\|Q8BGJ9\|U2AF4_MOUSE 100.0% — Splicing factor U2AF 26 kDa su...
Cadm1 `transcript807.9.nic`	ORF disrupted	Lost: DUF3439, MGC-24, Mid2, Mucin, SSP160	5prime_partial	11	21.6	sp\|Q8R5M8\|CADM1_MOUSE 90.5% — Cell adhesion molecule 1
Map3k10 `transcript872.7.nic`	ORF disrupted	Gained: Epsilon_antitox	5prime_partial	11	21.6	sp\|Q66L42\|M3K10_MOUSE 100.0% — Mitogen-activated protein kina...
Armc10 `transcript96.5.nnic`	ORF disrupted	Lost: TPR Gained: DUF5056	5prime_partial	11	21.6	—
Srebf2 `transcript1085.15.nnic`	ORF disrupted		5prime_partial	10	19.6	sp\|Q3U1N2\|SRBP2_MOUSE 100.0% — Sterol regulatory element-bind...
Pepd `transcript1298.7.nic`	ORF disrupted	Lost: AMP_N-like Gained: DUF3793	5prime_partial	9	17.6	sp\|Q11136\|PEPD_MOUSE 100.0% — Xaa-Pro dipeptidase
Lrrk2 `transcript1409.15.nic`	ORF disrupted	Lost: Ank, COR, LRR, P-loop_NTPase, PKinase, TPR Gained: MazG	5prime_partial	9	17.6	sp\|Q5S006\|LRRK2_MOUSE 100.0% — Leucine-rich repeat serine/thr...
Gabbr1 `transcript1422.17.nnic`	ORF disrupted	Lost: CCDC-167, COX1-like, DUF3995, DUF6377, GPCR_A, Kei1, NEMP, PKcGMP_CC, Periplas_BP, UPF0242	5prime_partial	9	17.6	sp\|Q9WV18\|GABR1_MOUSE 100.0% — Gamma-aminobutyric acid type B...
Zcchc17 `transcript1576.4.nnic`	ORF disrupted		5prime_partial	9	17.6	—

Frame Impact Classification

Functional Diversity per Gene

Top Pfam Domains

Domain Architectures per Gene

Annotation QC: Pfam Artifact Detection

Related Pfam families are grouped into clans; proteins with anomalously high domain density (structural protein artifacts) are flagged separately. The cleaned set is used for all downstream analysis.

Original DV Genes

1,032

After QC Filtering

745

Removed (Artifacts)

287

Flagged Details

162

Flagged Artifacts (162)

Gene	Gene Name	Reason	Arch (raw)	Arch (collapsed)	Core / Variable Domains
`ENSMUSG00000032366`	TPM4	density artifact	5	0	42 / 18
`ENSMUSG00000002985`	Apoe	density artifact	5	0	39 / 14
`ENSMUSG00000027940`	Tpm3	density artifact	4	0	45 / 13
`ENSMUSG00000014402`	Tsg101	density artifact	4	0	43 / 5
`ENSMUSG00000034160`	Ogt	density artifact	3	0	25 / 20
`ENSMUSG00000027506`	Tpd52	density artifact	3	0	28 / 12
`ENSMUSG00000055835`	Zfp1	density artifact	3	0	35 / 3
`ENSMUSG00000129504`	Nefm	density artifact	3	0	32 / 1
`ENSMUSG00000026927`	Entr1	density artifact	2	0	28 / 0
`ENSMUSG00000069208`	Znf431	density artifact	2	0	29 / 0
`ENSMUSG00000007207`	Stx1a	density artifact	2	0	53 / 0
`ENSMUSG00000026761`	Orc4	density artifact	2	0	52 / 0
`ENSMUSG00000045409`	Trim39	density artifact	2	0	36 / 0
`ENSMUSG00000019303`	Psmc3ip	density artifact	2	0	71 / 0
`ENSMUSG00000052033`	PFDN4	density artifact	2	0	50 / 0
`ENSMUSG00000031851`	Ntpcr	density artifact	2	0	41 / 0
`ENSMUSG00000064037`	Gpn1	density artifact	2	0	39 / 0
`ENSMUSG00000040242`	Fgfr1op2	density artifact	2	0	31 / 0
`ENSMUSG00000020708`	PSMC5	density artifact	2	0	37 / 0
`ENSMUSG00000001175`	CALM1	density artifact	2	0	31 / 0
`ENSMUSG00000078941`	Ak6	density artifact	2	0	38 / 0
`ENSMUSG00000024983`	Vti1a	density artifact	2	0	27 / 0
`ENSMUSG00000059689`	Znf32	density artifact	2	0	38 / 2
`ENSMUSG00000042097`	Znf239	density artifact	1	0	31 / 0
`ENSMUSG00000026966`	Ssna1	density artifact	1	0	55 / 0
`ENSMUSG00000020486`	Septin4	clan collapse	4	1	13 / 3
`ENSMUSG00000011658`	Fuz	clan collapse	4	1	3 / 0
`ENSMUSG00000051504`	CD33	clan collapse	5	2	7 / 0
`ENSMUSG00000033429`	Mcee	clan collapse	3	1	4 / 1
`ENSMUSG00000078765`	U2af1l4	clan collapse	3	1	7 / 0
`ENSMUSG00000032112`	Trappc4	clan collapse	4	2	4 / 0
`ENSMUSG00000069049`	Eif2s3y	clan collapse	4	2	8 / 0
`ENSMUSG00000026201`	Stk16	clan collapse	4	2	6 / 3
`ENSMUSG00000024645`	Timm21	clan collapse	2	1	2 / 0
`ENSMUSG00000038671`	Arfrp1	clan collapse	2	1	9 / 0
`ENSMUSG00000037062`	Sh3glb1	clan collapse	2	1	6 / 1
`ENSMUSG00000042729`	Wdr74	clan collapse	2	1	4 / 0
`ENSMUSG00000025173`	Wdr45b	clan collapse	2	1	7 / 0
`ENSMUSG00000026189`	Pecr	clan collapse	2	1	7 / 0
`ENSMUSG00000032475`	Nck1	clan collapse	2	1	8 / 0
`ENSMUSG00000025731`	Mettl26	clan collapse	2	1	6 / 1
`ENSMUSG00000030960`	Eef1akmt2	clan collapse	2	1	14 / 0
`ENSMUSG00000030272`	Camk1	clan collapse	2	1	9 / 0
`ENSMUSG00000029538`	Srsf9	clan collapse	2	1	5 / 0
`ENSMUSG00000033931`	Rbm34	clan collapse	2	1	7 / 0
`ENSMUSG00000026659`	Dusp12	clan collapse	2	1	4 / 0
`ENSMUSG00000000253`	Gmpr	clan collapse	2	1	1 / 3
`ENSMUSG00000025217`	Btrc	clan collapse	2	1	15 / 2
`ENSMUSG00000031144`	Syp	clan collapse	2	1	2 / 0
`ENSMUSG00000039018`	Mtg1	clan collapse	2	1	21 / 0
`ENSMUSG00000028032`	Papss1	clan collapse	2	1	14 / 0
`ENSMUSG00000007458`	M6pr	clan collapse	2	1	3 / 0
`ENSMUSG00000019718`	L3hypdh	clan collapse	2	1	2 / 0
`ENSMUSG00000090841`	Myl6	clan collapse	2	1	6 / 0
`ENSMUSG00000002778`	Kdelr1	clan collapse	2	1	2 / 0
`ENSMUSG00000029012`	Orc5	clan collapse	2	1	8 / 0
`ENSMUSG00000046434`	HNRNPA1	clan collapse	2	1	6 / 0
`ENSMUSG00000042540`	Acot5	clan collapse	2	1	5 / 0
`ENSMUSG00000025794`	Rpl14	clan collapse	2	1	3 / 0
`ENSMUSG00000005150`	Wdr83	clan collapse	2	1	4 / 2
`ENSMUSG00000019054`	Fis1	clan collapse	2	1	8 / 0
`ENSMUSG00000021094`	Dhrs7	clan collapse	2	1	5 / 0
`ENSMUSG00000017307`	Acot8	clan collapse	2	1	3 / 0
`ENSMUSG00000020736`	Nt5c	clan collapse	2	1	3 / 0
`ENSMUSG00000030512`	SNRPA1	clan collapse	2	1	4 / 0
`ENSMUSG00000045211`	Nudt18	clan collapse	2	1	2 / 0
`ENSMUSG00000052752`	TRAF7	clan collapse	2	1	25 / 0
`ENSMUSG00000063065`	Mapk3	clan collapse	2	1	9 / 0
`ENSMUSG00000051730`	Mettl5	clan collapse	2	1	23 / 0
`ENSMUSG00000044628`	Rnf208	clan collapse	2	1	10 / 0
`ENSMUSG00000025499`	H-RAS	clan collapse	2	1	13 / 0
`ENSMUSG00000026034`	Clk1	clan collapse	2	1	3 / 0
`ENSMUSG00000022474`	Pmm1	clan collapse	2	1	3 / 0
`ENSMUSG00000021852`	Slc35f4	clan collapse	2	1	2 / 0
`ENSMUSG00000020390`	UBE2B	clan collapse	2	1	3 / 0
`ENSMUSG00000024878`	Zng1	clan collapse	2	1	15 / 0
`ENSMUSG00000032583`	Mon1a	clan collapse	2	1	4 / 0
`ENSMUSG00000047067`	Dusp28	clan collapse	2	1	4 / 0
`ENSMUSG00000028954`	Nub1	clan collapse	2	1	11 / 0
`ENSMUSG00000031812`	Map1lc3b	clan collapse	2	1	3 / 0
`ENSMUSG00000040532`	Abhd11	clan collapse	2	1	10 / 4
`ENSMUSG00000053483`	Usp21	clan collapse	2	1	3 / 0
`ENSMUSG00000034807`	Colgalt1	clan collapse	2	1	4 / 0
`ENSMUSG00000039826`	Trub2	clan collapse	2	1	8 / 0
`ENSMUSG00000003559`	As3mt	clan collapse	2	1	18 / 0
`ENSMUSG00000047766`	Lrrc49	clan collapse	2	1	4 / 0
`ENSMUSG00000029166`	Mapre3	clan collapse	2	1	4 / 0
`ENSMUSG00000058240`	Cryzl1	clan collapse	2	1	3 / 0
`ENSMUSG00000006782`	Cnp	clan collapse	2	1	23 / 0
`ENSMUSG00000028099`	Polr3c	clan collapse	2	1	3 / 0
`ENSMUSG00000022677`	Cep20	clan collapse	2	1	2 / 0
`ENSMUSG00000021982`	Cdadc1	clan collapse	2	1	4 / 0
`ENSMUSG00000020385`	Clk4	clan collapse	2	1	5 / 0
`ENSMUSG00000039637`	Coro7	clan collapse	2	1	10 / 0
`ENSMUSG00000020873`	Slc35b1	clan collapse	2	1	5 / 0
`ENSMUSG00000059974`	Ntm	clan collapse	2	1	12 / 0
`ENSMUSG00000041429`	Nthl1	clan collapse	2	1	4 / 0
`ENSMUSG00000006728`	Cdk4	clan collapse	2	1	8 / 0
`ENSMUSG00000004032`	Gstm5	clan collapse	2	1	6 / 0
`ENSMUSG00000034744`	Nagk	clan collapse	2	1	1 / 1
`ENSMUSG00000078923`	Ube2v1	clan collapse	2	1	2 / 0
`ENSMUSG00000031505`	Naxd	clan collapse	2	1	3 / 0
`ENSMUSG00000032336`	Nptn	clan collapse	2	1	10 / 0
`ENSMUSG00000022130`	Tgds	clan collapse	2	1	9 / 0
`ENSMUSG00000054855`	Rnd1	clan collapse	2	1	6 / 0
`ENSMUSG00000052456`	Get3	clan collapse	2	1	15 / 0
`ENSMUSG00000021111`	Papola	clan collapse	2	1	4 / 0
`ENSMUSG00000026427`	Eif2d	clan collapse	2	1	8 / 1
`ENSMUSG00000024799`	Tm7sf2	clan collapse	2	1	4 / 0
`ENSMUSG00000025939`	Ube2w	clan collapse	2	1	3 / 0
`ENSMUSG00000028969`	CDK5	clan collapse	2	1	7 / 0
`ENSMUSG00000029068`	Ccnl2	clan collapse	2	1	5 / 0
`ENSMUSG00000024168`	Tmem204	clan collapse	2	1	7 / 0
`ENSMUSG00000003346`	Abhd17a	clan collapse	2	1	14 / 0
`ENSMUSG00000024524`	GNAL	clan collapse	2	1	9 / 0
`ENSMUSG00000058291`	Zfp39	clan collapse	2	1	9 / 1
`ENSMUSG00000071379`	Hpcal1	clan collapse	2	1	11 / 0
`ENSMUSG00000021548`	Ccnh	clan collapse	2	1	3 / 0
`ENSMUSG00000022024`	Sugt1	clan collapse	2	1	18 / 0
`ENSMUSG00000028252`	Ccnc	clan collapse	2	1	4 / 0
`ENSMUSG00000051007`	Gatd1	clan collapse	2	1	2 / 0
`ENSMUSG00000045136`	TUBB2B	clan collapse	2	1	3 / 0
`ENSMUSG00000039345`	Mettl22	clan collapse	2	1	6 / 0
`ENSMUSG00000022635`	Zcrb1	clan collapse	2	1	6 / 0
`ENSMUSG00000030298`	Sec13	clan collapse	2	1	5 / 0
`ENSMUSG00000037894`	H2AZ1	clan collapse	2	1	3 / 0
`ENSMUSG00000063229`	Ldha	clan collapse	2	1	4 / 0
`ENSMUSG00000034209`	Rasl10a	clan collapse	2	1	7 / 0
`ENSMUSG00000030701`	Plekhb1	clan collapse	2	1	3 / 0
`ENSMUSG00000027998`	Plrg1	clan collapse	2	1	9 / 0
`ENSMUSG00000018593`	Sparc	clan collapse	2	1	7 / 1
`ENSMUSG00000016637`	Ift27	clan collapse	2	1	7 / 0
`ENSMUSG00000017715`	Pgs1	clan collapse	2	1	3 / 0
`ENSMUSG00000036067`	Slc2a6	clan collapse	2	1	2 / 1
`ENSMUSG00000024576`	CSNK1A1	clan collapse	2	1	7 / 0
`ENSMUSG00000032786`	Alas1	clan collapse	2	1	5 / 0
`ENSMUSG00000068250`	Amn1	clan collapse	2	1	3 / 1
`ENSMUSG00000021772`	Nkiras1	clan collapse	2	1	9 / 0
`ENSMUSG00000024862`	KLC2	clan collapse	2	1	38 / 0
`ENSMUSG00000035142`	Nubpl	clan collapse	2	1	10 / 0
`ENSMUSG00000038975`	Rabggtb	clan collapse	2	1	3 / 0
`ENSMUSG00000042831`	Alkbh6	clan collapse	2	1	1 / 1
`ENSMUSG00000049225`	Pdp1	clan collapse	2	1	2 / 0
`ENSMUSG00000024516`	Sec11c	clan collapse	2	1	2 / 0
`ENSMUSG00000069835`	Sat2	clan collapse	2	1	8 / 0
`ENSMUSG00000037351`	Actr1b	clan collapse	2	1	2 / 0
`ENSMUSG00000041438`	Utp4	clan collapse	2	1	10 / 0
`ENSMUSG00000022971`	Ifnar2	clan collapse	2	1	4 / 0
`ENSMUSG00000055720`	Ubl7	clan collapse	2	1	6 / 0
`ENSMUSG00000002524`	Puf60	clan collapse	2	1	5 / 0
`ENSMUSG00000019295`	Tmem129	clan collapse	2	1	4 / 0
`ENSMUSG00000038286`	Bphl	clan collapse	2	1	11 / 1
`ENSMUSG00000023051`	Tarbp2	clan collapse	2	1	4 / 0
`ENSMUSG00000013622`	Atraid	clan collapse	2	1	4 / 0
`ENSMUSG00000022453`	Naga	clan collapse	2	1	4 / 0
`ENSMUSG00000014554`	Dguok	clan collapse	2	1	5 / 0
`ENSMUSG00000087260`	Lamtor5	clan collapse	2	1	2 / 0
`ENSMUSG00000056673`	Kdm5d	clan collapse	2	1	12 / 0
`ENSMUSG00000019188`	Hm13	clan collapse	2	1	4 / 0
`ENSMUSG00000023755`	Rhebl1	clan collapse	2	1	8 / 0
`ENSMUSG00000022503`	Nubp1	clan collapse	2	1	25 / 0
`ENSMUSG00000022570`	Gfus	clan collapse	2	1	6 / 0

Domain Variants by Evidence Tier

Variable Domain Landscape

Total Variable Domains

409

Domain Loss Events

396

Domain Gain Events

Loss : Gain Ratio

7.6:1

Domain loss dominates gain ~8:1, consistent with alternative splicing predominantly removing exons encoding specific domains rather than inserting novel domain-encoding exons.

All Variable Domains (409)

Domain	DV Genes	Gain	Loss	Genes
`DUF2207`	4	0	4	Armc10, Set, Tmem134, Tmem208
`Exonuc_VII_L`	3	0	3	Bloc1s5, Olfm1, Septin8
`DUF1664`	3	0	3	Bin1, Immt, Olfm1
`ABC1`	3	1	2	Rps6kb2, Speg, Araf
`Pkinase_fungal`	3	1	2	Rps6kb2, Speg, Araf
`Presenilin`	3	2	1	U2AF2, Cd47, RNPS1
`Kinase-like`	3	1	2	Rps6kb2, Stk16, Araf
`CCDC-167`	2	1	1	becn1, Gabbr1
`Jnk-SapK_ap_N`	2	0	2	Bin1, Stx5
`Syntaxin_2`	2	0	2	Olfm1, Stx5
`PDZ`	2	0	2	ENSMUSG00000024227, Htra2
`PDZ_6`	2	0	2	ENSMUSG00000024227, Htra2
`Peptidase_M50`	2	0	2	ENSMUSG00000024227, Htra2
`PDZ_2`	2	0	2	ENSMUSG00000024227, Htra2
`AAA_13`	2	0	2	Olfm1, Tpd52l2
`ZapB`	2	0	2	Olfm1, Set
`Csm1_N`	2	0	2	Olfm1, Stx4
`FUSC`	2	0	2	Dtnbp1, Olfm1
`Peptidase_S46`	2	0	2	Cops7b, Dtnbp1
`Elf1`	2	0	2	Cacfd1, YPEL3
`Epimerase`	2	0	2	Qdpr, Sdr39u1
`Kdo`	2	1	1	Speg, Araf
`Pkinase`	2	1	1	Speg, Araf
`APH`	2	1	1	Speg, Araf
`PK_Tyr_Ser-Thr`	2	1	1	Speg, Araf
`FTA2`	2	1	1	Rps6kb2, Phkg2
`EMP24_GP25L`	2	0	2	Set, VAMP4
`DUF4407`	2	1	1	Eif2a, Hnrnpc
`PH_13`	2	0	2	ENSMUSG00000024227, Rasgrf1
`OmpH`	2	0	2	Bin1, Set
`DUF4191`	2	0	2	Cacfd1, Tmem208
`TMF_TATA_bd`	1	0	1	Klc1
`DUF349`	1	0	1	Hmox2
`PKcGMP_CC`	1	1	0	Gabbr1
`Peripla_BP_6`	1	1	0	Gabbr1
`7tm_3`	1	1	0	Gabbr1
`ANF_receptor`	1	1	0	Gabbr1
`DUF6377`	1	1	0	Gabbr1
`NEMP`	1	1	0	Gabbr1
`UPF0242`	1	1	0	Gabbr1
`COX1`	1	1	0	Gabbr1
`DUF3995`	1	1	0	Gabbr1
`Kei1`	1	1	0	Gabbr1
`GvpO`	1	0	1	Nfs1
`Peptidase_C48`	1	0	1	Nfs1
`EH_Signature`	1	0	1	Nfs1
`Pyridoxal_deC`	1	0	1	Nfs1
`SPRY`	1	0	1	Trim9
`PRY`	1	0	1	Trim9
`Nuf2_DHR10-like`	1	0	1	Stx5
`DUF4795`	1	0	1	Stx5
`Cep3`	1	0	1	Stx5
`Prominin`	1	0	1	Stx5
`QWRF`	1	0	1	Stx5
`WASH_WAHD`	1	0	1	Stx5
`Baculo_F`	1	0	1	Stx5
`PhageMetallopep`	1	1	0	Mindy3
`Redoxin`	1	0	1	Rpl34
`DUF202`	1	0	1	Ndfip2
`HLH`	1	0	1	Mrpl58
`TUTase`	1	0	1	Mtpap
`CXCXC`	1	0	1	Vegfb
`VEGF_C`	1	0	1	Vegfb
`DUF3911`	1	0	1	Paip2
`DUF6592`	1	0	1	Cuedc2
`AAR2`	1	0	1	Use1
`CDP-OH_P_tran_2`	1	0	1	Mtch2
`FXMRP1_C_core`	1	0	1	Drap1
`Glucosaminidase`	1	0	1	Tardbp
`SelR`	1	0	1	Slirp
`zf-RING_10`	1	0	1	Mkrn1
`GlcNAc-1_reg`	1	0	1	Tcp1
`MLD`	1	0	1	Iqcb1
`GPS2_interact`	1	0	1	Mrps15
`UPF0181`	1	0	1	Sf1
`DUF4726`	1	0	1	Hspbp1
`Stathmin`	1	0	1	Bloc1s5
`APC_rep`	1	0	1	Bloc1s5
`Phage_int_SAM_1`	1	0	1	KCTD1
`DUF3504`	1	0	1	KCTD1
`DUF621`	1	0	1	Gabrd
`PPP4R2`	1	0	1	Apbb1
`DNA_pol_phi`	1	0	1	Apbb1
`GRASP55_65`	1	0	1	Htra2
`Peptidase_S32`	1	0	1	Htra2
`Methyltransf_10`	1	0	1	Tfdp1
`Neurexophilin`	1	0	1	Tfdp1
`DUF4199`	1	0	1	Mmd2
`Ceramidase`	1	0	1	Mmd2
`DUF2813`	1	0	1	Pak1
`Microtub_bd`	1	0	1	Septin8
`ATG16`	1	0	1	Septin8
`TPR_6`	1	0	1	Anapc5
`TPR_11`	1	0	1	Anapc5
`Ham1p_like`	1	0	1	Anapc5
`Lys_Orn_oxgnase`	1	0	1	Txnrd2
`Lycopene_cycl`	1	0	1	Txnrd2
`VPS38`	1	0	1	becn1
`PI_PP_I`	1	0	1	becn1
`PP1c_bdg`	1	0	1	becn1
`Sec2p`	1	0	1	becn1
`Glu_dehyd_C`	1	0	1	Smox
`Trp_halogenase`	1	0	1	Smox
`MLTD_N`	1	0	1	Smox
`NESP55`	1	0	1	Gnas
`Peptidase_S49_N`	1	0	1	Gnas
`MARVEL`	1	0	1	Gnas
`AAA_24`	1	0	1	Gnas
`MMR_HSR1`	1	0	1	Gnas
`DUF4520`	1	0	1	ENSMUSG00000062822
`Lipocalin`	1	0	1	ENSMUSG00000062822
`DUF3772`	1	0	1	RPL19
`Sin3_corepress`	1	0	1	Sin3b
`Sin3a_C`	1	0	1	Sin3b
`DUF5077`	1	0	1	Sin3b
`HTH_20`	1	0	1	Myl4
`SH3_12`	1	0	1	Fubp1
`Occludin_ELL`	1	0	1	Olfm1
`PilJ`	1	0	1	Olfm1
`OLF`	1	0	1	Olfm1
`Helo_like_N`	1	0	1	Olfm1
`DUF4446`	1	0	1	Olfm1
`EzrA`	1	0	1	Olfm1
`DUF2203`	1	0	1	Olfm1
`TBCC_N`	1	0	1	Olfm1
`SlyX`	1	0	1	Olfm1
`Nup54`	1	0	1	Olfm1
`PriC`	1	0	1	Olfm1
`Laminin_II`	1	0	1	Olfm1
`H-kinase_dim`	1	0	1	Olfm1
`NPV_P10`	1	0	1	Olfm1
`Enkurin`	1	0	1	Olfm1
`ABC_tran_CTD`	1	0	1	Olfm1
`HSBP1`	1	0	1	Olfm1
`ATG17_like`	1	0	1	Olfm1
`Myosin_tail_1`	1	0	1	Olfm1
`Cytochrom_B562`	1	0	1	Olfm1
`DUF5917`	1	0	1	Olfm1
`Baculo_PEP_C`	1	0	1	Olfm1
`DUF6161`	1	0	1	CLTA
`FAM184`	1	0	1	CLTA
`TT_ORF2a`	1	0	1	Rab36
`DUF3188`	1	0	1	Nudt6
`DUF2417`	1	0	1	Tmub2
`XRN1_DBM`	1	0	1	Tmub2
`DUF2730`	1	0	1	Dtnbp1
`GWT1`	1	0	1	Armc10
`Atx10homo_assoc`	1	0	1	Armc10
`CrtO`	1	0	1	Armc10
`DUF5056`	1	0	1	Armc10
`Arm_2`	1	0	1	Armc10
`DUF3899`	1	0	1	Armc10
`Arm`	1	0	1	Armc10
`V-ATPase_H_C`	1	0	1	Armc10
`DUF6548`	1	0	1	Armc10
`Orf78`	1	0	1	Armc10
`U-box`	1	0	1	Stub1
`zf-NOSIP`	1	0	1	Stub1
`TPR_20`	1	0	1	Stub1
`DiS_P_DiS`	1	0	1	YPEL3
`RINGv`	1	0	1	Rnf13
`DNA_ligase_ZBD`	1	0	1	Rnf13
`SCHIP-1`	1	0	1	Rnf13
`CTU2`	1	0	1	Rnf13
`zf-RING_16`	1	0	1	Rnf13
`zf-C3HC4_3`	1	0	1	Rnf13
`PHD`	1	0	1	Rnf13
`zf-C3H2C3`	1	0	1	Rnf13
`FAM176`	1	0	1	Rnf13
`zf-RING_4`	1	0	1	Rnf13
`Prok-RING_4`	1	0	1	Rnf13
`HTH_63`	1	0	1	Rnf13
`Baculo_11_kDa`	1	0	1	Rnf13
`zf-ANAPC11`	1	0	1	Rnf13
`zf-rbx1`	1	0	1	Rnf13
`Lipoprotein_21`	1	0	1	Ube2v1
`Ribosomal_L9_N`	1	0	1	P4htm
`Yip1`	1	0	1	Tusc3
`DUF6350`	1	0	1	Tusc3
`YhhN`	1	0	1	Tusc3
`Bax1-I`	1	0	1	Tusc3
`SR1P`	1	0	1	Stx18
`PRMT5`	1	0	1	Qdpr
`YopD`	1	1	0	Fam107a
`LMBR1`	1	0	1	Psma3
`DUF5320`	1	0	1	Snf8
`ZapD`	1	0	1	Snf8
`Cytochrom_C_2`	1	0	1	Eef1d
`Herpes_DNAp_acc`	1	0	1	Eef1d
`Mo25`	1	0	1	Chmp2a
`GEN1_C`	1	0	1	Nap1l1
`CBF`	1	0	1	Nap1l1
`NPL`	1	1	0	Nap1l1
`Mid2`	1	0	1	Cadm1
`MGC-24`	1	0	1	Cadm1
`Mucin`	1	0	1	Cadm1
`C1-set`	1	0	1	Speg
`Receptor_2B4`	1	0	1	Speg
`Izumo-Ig`	1	0	1	Speg
`Pox_ser-thr_kin`	1	0	1	Speg
`Ig_6`	1	0	1	Speg
`fn3`	1	0	1	Speg
`Ig_4`	1	0	1	Speg
`CCDC85`	1	0	1	Atp6v0a1
`AlkA_N`	1	0	1	Atp6v0a1
`FliG_N`	1	0	1	Phkg2
`PMSI1`	1	0	1	Rnaseh2a
`TSP_1`	1	0	1	Rnaseh2a
`CoV_nucleocap`	1	0	1	U2AF2
`NPR3`	1	0	1	U2AF2
`SMN`	1	0	1	U2AF2
`DNA_primase_lrg`	1	1	0	Rpl18a
`Cactin_mid`	1	0	1	Necab3
`CCDC23`	1	1	0	Josd2
`Rubredoxin_2`	1	1	0	Araf
`DUF2457`	1	0	1	RNPS1
`Nup35_RRM_2`	1	0	1	HNRNPA2B1
`Laminin_G_1`	1	0	1	HNRNPA2B1
`CDC24`	1	0	1	Aplp2
`APP_amyloid`	1	0	1	Aplp2
`RapH_N`	1	0	1	Aplp2
`DUF1285`	1	0	1	GAL3ST4
`Phage_holin_3_6`	1	0	1	Rtn4
`FAM76`	1	0	1	Srsf5
`MAJIN`	1	0	1	Srsf5
`PRP38_assoc`	1	0	1	Srsf5
`FAM104`	1	0	1	FAM156A
`Het-C`	1	0	1	Sgce
`DUF2057`	1	0	1	Lin7b
`DUF4748`	1	0	1	Pnkd
`DUF948`	1	0	1	VAMP4
`DUF5070`	1	1	0	Rbm3
`MogR_DNAbind`	1	0	1	Tatdn3
`PCAF_N`	1	0	1	Pea15
`MetOD2`	1	0	1	Naa10
`FSA_C`	1	0	1	Khdrbs3
`RR_TM4-6`	1	0	1	Zcchc17
`CCDC84`	1	0	1	Stmn4
`AAA_23`	1	0	1	Stmn4
`GBP_C`	1	0	1	Stmn4
`KleE`	1	0	1	Fus
`DUF1106`	1	1	0	RPL22
`VP1_VP3`	1	0	1	Serbp1
`COX4`	1	0	1	Plpp1
`DUF6541`	1	0	1	Plpp1
`DUF418`	1	0	1	Plpp1
`IQ`	1	0	1	Rasgrf1
`RhoGEF`	1	0	1	Rasgrf1
`PH_17`	1	0	1	Rasgrf1
`AAA_11`	1	0	1	Rasgrf1
`DUF3877`	1	0	1	Rasgrf1
`RasGEF`	1	0	1	Rasgrf1
`RasGEF_N`	1	0	1	Rasgrf1
`DUF4133`	1	0	1	Psmd9
`PH_16`	1	0	1	ENSMUSG00000024227
`HTH_ABP1_N`	1	0	1	ENSMUSG00000024227
`PH_20`	1	0	1	ENSMUSG00000024227
`CtnDOT_TraJ`	1	0	1	Tpd52l1
`Binary_toxB_2`	1	0	1	Tpd52l1
`Uso1_p115_C`	1	0	1	Tpd52l1
`Fib_alpha`	1	0	1	Tpd52l1
`Reg_prop`	1	0	1	Cdc123
`CK2S`	1	0	1	Bin1
`BAR_3`	1	0	1	Bin1
`V-SNARE`	1	0	1	Bin1
`TBD`	1	0	1	Bin1
`Arfaptin`	1	0	1	Bin1
`DUF3138`	1	0	1	Dda1
`Lgl_C`	1	0	1	Dda1
`Anthrone_oxy`	1	0	1	Plpp5
`MeaB`	1	0	1	Pstk
`AAA_22`	1	0	1	Pstk
`DO-GTPase1`	1	0	1	Pstk
`AAA_33`	1	0	1	Pstk
`ParA`	1	0	1	Pstk
`AAA_25`	1	0	1	Pstk
`APS_kinase`	1	0	1	Pstk
`AAA_18`	1	0	1	Pstk
`Zeta_toxin`	1	0	1	Pstk
`AAA_16`	1	0	1	Pstk
`DEAD_assoc`	1	0	1	Pstk
`SRP9-21`	1	0	1	SUMO2
`DUF5010_C`	1	0	1	SUMO2
`DUF3671`	1	0	1	Stx4
`T2SSF`	1	0	1	Stx4
`HisKA_3`	1	0	1	Stx4
`DUF6584`	1	0	1	Stx4
`TPR_MLP1_2`	1	0	1	Stx4
`PRP1_N`	1	0	1	Stx4
`Vac_Fusion`	1	0	1	Stx4
`COG2`	1	0	1	Stx4
`DUF4094`	1	0	1	Stx4
`Psg1`	1	0	1	Stx4
`ETRAMP`	1	0	1	Stx4
`Rx_N`	1	0	1	Immt
`FAT`	1	0	1	Immt
`ARGLU`	1	0	1	Immt
`SBP_bac_11`	1	0	1	Immt
`DUF2613`	1	0	1	Tspan17
`DNA_pol_lambd_f`	1	0	1	Polb
`HHH`	1	0	1	Polb
`5_3_exonuc`	1	1	0	Polb
`HHH_3`	1	0	1	Polb
`HHH_2`	1	0	1	Polb
`Cdd1`	1	0	1	Polb
`IMS_HHH`	1	0	1	Polb
`DUF3701`	1	0	1	Polb
`WSN`	1	0	1	Polb
`HHH_5`	1	0	1	Polb
`bZIP_Maf`	1	1	0	Sh3glb2
`DAG_kinase_N`	1	0	1	Zfand3
`FYVE`	1	0	1	Zfand3
`Nucleo_P87`	1	0	1	Zfand3
`ABC_cobalt`	1	0	1	Cacfd1
`DUF1450`	1	0	1	Nherf2
`DUF6288`	1	0	1	Nherf2
`PRIMA1`	1	0	1	Tmem19
`AIG1`	1	0	1	Eef1a2
`Septin`	1	0	1	Eef1a2
`TetR_C_24`	1	0	1	Dctn3
`DivIC`	1	0	1	Dctn3
`DUF2755`	1	0	1	Gpm6b
`Glyco_hydro_36`	1	0	1	Tpd52l2
`DUF4164`	1	0	1	Tpd52l2
`Ge1_WD40`	1	0	1	Fbxw2
`CHAP`	1	0	1	Fbxw2
`Nup88`	1	0	1	Hnrnpc
`Cytadhesin_P30`	1	0	1	Hnrnpc
`PA26`	1	1	0	Hnrnpc
`TMPIT`	1	0	1	Hnrnpc
`RmlD_sub_bind`	1	0	1	Sdr39u1
`NAD_binding_10`	1	0	1	Sdr39u1
`Pyr_redox`	1	0	1	Sdr39u1
`GDP_Man_Dehyd`	1	0	1	Sdr39u1
`LCD1`	1	1	0	Thyn1
`PX`	1	0	1	Sgk1
`PTN_MK_N`	1	0	1	Shisa5
`SOBP`	1	0	1	Dync1i2
`DUF3489`	1	0	1	Rpl13a
`BAR_2`	1	0	1	Arfip2
`DUF3040`	1	0	1	Tmem134
`Virul_fac_BrkB`	1	1	0	Tmem134
`DUF6232`	1	1	0	Tmem134
`ThrE_2`	1	1	0	Tmem134
`Neurensin`	1	0	1	Tmem134
`DUF308`	1	0	1	Tmem134
`TMEM171`	1	1	0	Tmem134
`SdpI`	1	0	1	Tmem134
`DPM3`	1	1	0	Tmem134
`zf-RanBP`	1	0	1	Ttc33
`CCD97-like_C`	1	0	1	Ttc33
`DUF2112`	1	0	1	Ttc33
`ExbD`	1	0	1	Ramp2
`ESCRT-II`	1	0	1	Ramp2
`SARAF`	1	1	0	Stk16
`DUF5898`	1	1	0	Stk16
`F-box-like`	1	0	1	Fbxl6
`F-box`	1	0	1	Fbxl6
`eIF-3_zeta`	1	0	1	Fxr1
`DUF4629`	1	0	1	Fxr1
`Tfb5`	1	0	1	Ndufab1
`YrbL-PhoP_reg`	1	0	1	RPS24
`AGOG`	1	0	1	Cops7b
`PTPRCAP`	1	0	1	Rps6kb2
`DUF6264`	1	0	1	Rps6kb2
`DUF3446`	1	1	0	Cd47
`SSP160`	1	1	0	Cd47
`SR-25`	1	1	0	Cd47
`IMUP`	1	1	0	Cd47
`Raf1_HTH`	1	1	0	Ubc
`Phage_sheath_1N`	1	0	1	Ubc
`DMA`	1	0	1	Ubc
`Sde2_N_Ubi`	1	1	0	Ubc
`DUF969`	1	0	1	Ubc
`TraK`	1	0	1	Ubc
`2_5_RNA_ligase2`	1	0	1	Ubc
`GDYXXLXY`	1	1	0	Ubc
`FlbT`	1	1	0	Hdhd2
`STAS`	1	1	0	Hdhd2
`FleQ`	1	1	0	Hdhd2
`Tegument_dsDNA`	1	0	1	Cars1
`DUF1645`	1	0	1	Uimc1
`ubiquitin`	1	0	1	Tecr
`MapZ_C2`	1	0	1	Dpm1
`T2SSM`	1	1	0	UBE2I
`DUF6493`	1	0	1	Mbd3
`HSP70`	1	0	1	Kars1
`Ribophorin_I`	1	0	1	Rpusd1
`DUF3742`	1	0	1	Atp6v0b
`YbgT_YccB`	1	0	1	Atp6v0b
`DUF815`	1	0	1	Dap3
`DUF4688`	1	0	1	Dap3
`HXXEE`	1	0	1	Set
`EMP70`	1	0	1	Set
`DUF6279`	1	0	1	Set
`DUF3149`	1	0	1	Set
`DUF1129`	1	0	1	Set
`RseC_MucC`	1	0	1	Set
`Nore1-SARAH`	1	0	1	Set
`DUF6677`	1	0	1	Clu
`FAM192A_Fyv6_N`	1	0	1	Clu
`RIO1`	1	0	1	CAMK2A
`DUF6590`	1	0	1	CAMK2A
`UPF0449`	1	0	1	Bloc1s2
`Sec1`	1	0	1	Bloc1s2
`nos_propeller`	1	0	1	Slc3a2
`AHD`	1	0	1	Slc3a2
`Bee_toxin`	1	0	1	Tmem33
`PRR20`	1	0	1	Fam241b

Domain Variant Genes (745 genes, multiple architectures after QC)

All domain variant genes retaining multiple domain architectures after clan collapse and density artifact removal. Use the search box to filter by gene ID, name, function, or domain.

Showing 745 of 745 genes

Gene	Name	Function	Isoforms	Arch	Tier	CPM	Source	Core Domains
`ENSMUSG00000008348`	Ubc	Polyubiquitin-C	7	5	1	152.8	Novel	Big_7, CFAP298, CorA, Crinkler, DUF1942, DUF2604, DUF3861, DUF5450, DUF6393, Dsc3_N, FlgD_ig, HemeBinding_Shp, LBP_C, ORF11CD3, Plexin_RBD, ProRS-C_2, Pullulanase_N2, Rad60-SLD, Rad60-SLD_2, Staphylokinase, TBK1_ULD, TmoB, UDP-g_GGTase, Ubiquitin_2, Ubiquitin_4, Ubiquitin_5, ubiquitin
`ENSMUSG00000021288`	Klc1	Kinesin light chain 1	4	4	1	403.5	Mixed	ANAPC3, COG2, DUF4919, DUF627, GHL5, GlfT2_domain3, JIP_LZII, PPR, PPR_2, RNPP_C, RPN7, SHNi-TPR, SOGA, TPR_1, TPR_10, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_4, TPR_7, TPR_8, TPR_MalT, YabA
`ENSMUSG00000029054`	Gabrd	Gamma-aminobutyric acid receptor subunit delta	4	4	1	115.6	Mixed	Neur_chan_LBD, Neur_chan_memb, PgaD, PsbY
`ENSMUSG00000006675`	P4htm	Transmembrane prolyl 4-hydroxylase	4	4	1	211.5	Mixed	2OG-FeII_Oxy_3, EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, TruB_C
`ENSMUSG00000006058`	Snf8	Vacuolar-sorting protein SNF8	4	4	1	166.5	Mixed	DUF4896, EAP30, EutK_C, Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C
`ENSMUSG00000027489`	Necab3	N-terminal EF-hand calcium-binding protein 3	4	4	1	109.7	Novel	ABM, DnaI_N, EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, Jnk-SapK_ap_N
`ENSMUSG00000030805`	Stx4	Syntaxin-4	4	4	1	23.5	Novel	AAA_13, ATG17_like, BLOC1_2, Baculo_PEP_C, COG5, DUF16, DUF1664, DUF3698, DUF5798, Desmo_N, EMP24_GP25L, ERp29, GlutR_dimer, Lectin_N, LuxT_C, NPV_P10, PilO, SNARE, Syntaxin, Syntaxin_2
`ENSMUSG00000000296`	Tpd52l1	Tumor protein D53	4	4	4	19.6	Mixed	Cnn_1N, CorA, DUF1818, DUF3458_C, DUF745, NUDE_C, TMEM247, TPD52, Uds1, WEMBL
`ENSMUSG00000027618`	Nfs1	Cysteine desulfurase	3	3	1	50.9	Mixed	Aminotran_5, Beta_elim_lyase, DegT_DnrJ_EryC1
`ENSMUSG00000024914`	Drap1	Dr1-associated corepressor	3	3	1	266.4	Ref	CBFD_NFYB_HMF, Histone, PPP4R2, TAF4
`ENSMUSG00000041459`	Tardbp	TAR DNA-binding protein 43	3	3	1	0.0	Ref	Nup35_RRM_2, RL, RRM_1, RRM_3, RRM_5, RRM_7, TDP43_N, Trypsin
`ENSMUSG00000021040`	Slirp	SRA stem-loop-interacting RNA-binding protein, mitochondrial	3	3	1	27.4	Ref	Nup35_RRM, Nup35_RRM_2, PB1, RRM_1
`ENSMUSG00000028861`	Mrps15	Small ribosomal subunit protein uS15m	3	3	1	184.1	Novel	DUF272, DUF4140, DUF724, MIX23, PIN_4, Ribosomal_S15
`ENSMUSG00000035642`	Aamdc	Mth938 domain-containing protein	3	3	1	107.7	Ref	DUF498, Indigoidine_A, UPF0180
`ENSMUSG00000038982`	Bloc1s5	Biogenesis of lysosome-related organelles complex 1 subunit 5	3	3	1	3.9	Mixed	DUF5557, Muted
`ENSMUSG00000039533`	Mmd2	Monocyte to macrophage differentiation factor 2	3	3	1	33.3	Novel	DUF2842, Ferlin_C, HlyIII
`ENSMUSG00000022884`	EIF4A2	Eukaryotic initiation factor 4A-II	4	3	1	850.1	Ref	AAA_19, AAA_22, AAA_30, CMS1, DEAD, Fanconi_A, Flavi_DEAD, Helicase_C, Helicase_RecD, ResIII, UvrD-helicase, tRNA-synt_1b
`ENSMUSG00000029472`	Anapc5	Anaphase-promoting complex subunit 5	4	3	1	80.3	Ref	ANAPC5, MapZ_EC1, TPR_1, TPR_10, TPR_12, TPR_14, TPR_17, TPR_19, TPR_2, TPR_4, TPR_7, VP4_haemagglut, YycI
`ENSMUSG00000035086`	becn1	Beclin-1	4	3	1	21.5	Mixed	ALMT, APG6, APG6_N, BH3, DASH_Hsk3, DUF3450, DUF4164, DUF4200, Exonuc_VII_L, FapA, GAS, HAUS-augmin3, MT, NAPRTase_C, Suppressor_APC, V_ATPase_I, YabA
`ENSMUSG00000027523`	Gnas	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	3	3	1	513.2	Ref	AAA_29, Arf, G-alpha, GTP_EFTU, Gtr1_RagA, Roc, TniB
`ENSMUSG00000062822`	—	Uncharacterized protein C5orf34 homolog	3	3	1	3.9	Mixed	DUF4524
`ENSMUSG00000026833`	Olfm1	Noelin	3	3	1	871.6	Ref	BLOC1_2, CENP-Q, CLZ, DUF3450, Ephrin, FtsZ_C, MitMem_reg, Noelin-1, XhlA
`ENSMUSG00000028478`	CLTA	Clathrin light chain A	4	3	1	528.8	Ref	CENP-Q, Clathrin_lg_ch, ORC3_N
`ENSMUSG00000034757`	Tmub2	Transmembrane and ubiquitin-like domain-containing protein 2	3	3	1	82.3	Ref	CD52, DUF6232, Dsc3_N, Rad60-SLD_2, ubiquitin
`ENSMUSG00000038525`	Armc10	Armadillo repeat-containing protein 10	3	3	1	13.7	Novel	—
`ENSMUSG00000036503`	Rnf13	E3 ubiquitin-protein ligase RNF13	3	3	1	15.7	Ref	FeoB_associated, LapA_dom, Neur_chan_memb, Ninjurin, PA, SID-1_RNA_chan, zf-C3HC4, zf-C3HC4_2, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000118664`	Tusc3	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3	3	3	1	70.5	Mixed	DUF4131, Gram_pos_anchor, OST3_OST6, Thioredoxin
`ENSMUSG00000058799`	Nap1l1	Nucleosome assembly protein 1-like 1	6	3	1	313.4	Novel	NAP
`ENSMUSG00000032076`	Cadm1	Cell adhesion molecule 1	4	3	1	9.8	Mixed	Adeno_E3_CR1, Adhes-Ig_like, C1-set, C2-set, C2-set_2, DUF3439, I-set, Ig_2, Ig_3, Ig_4, Ig_C17orf99, Izumo-Ig, SSP160, Syndecan, V-set, ig
`ENSMUSG00000030815`	Phkg2	Phosphorylase b kinase gamma catalytic chain, liver/testis isoform	6	3	1	41.1	Mixed	ABC1, APH, Choline_kinase, DUF2805, Haspin_kinase, Kdo, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Pox_ser-thr_kin, RIO1, TCAD9, YrbL-PhoP_reg
`ENSMUSG00000001127`	Araf	Serine/threonine-protein kinase A-Raf	6	3	1	178.2	Mixed	C1_1, RBD, TUG-UBL1, zf-RING-like, zf-RING_2
`ENSMUSG00000034681`	RNPS1	RNA-binding protein with serine-rich domain 1	7	3	1	94.0	Mixed	Macoilin, Nup35_RRM_2, PI3K_1B_p101, RRM_1, RRM_3, RRM_5, RRM_7, RRM_occluded
`ENSMUSG00000004980`	HNRNPA2B1	Heterogeneous nuclear ribonucleoproteins A2/B1	4	3	1	31.3	Novel	HnRNPA1, OB_RNB, RRM_1, RRM_7
`ENSMUSG00000031996`	Aplp2	Amyloid beta precursor like protein 2	3	3	1	148.9	Novel	APP_Cu_bd, APP_E2, APP_N, Activator-TraM, CDC45, DabA, Kunitz_BPTI, VHL_C
`ENSMUSG00000085227`	GAL3ST4	Galactose-3-O-sulfotransferase 4	3	3	1	7.8	Ref	FmdE, Gal-3-0_sulfotr
`ENSMUSG00000020458`	Rtn4	Reticulon-4	3	3	1	423.1	Novel	DUF4736, DUF639, Reticulon
`ENSMUSG00000019923`	Zwint	Outer kinetochore KNL1 complex subunit ZWINT	4	3	1	1,394.6	Mixed	FHIPEP, HEF_HK, Noelin-1, Nuf2_DHR10-like, Zwint
`ENSMUSG00000031388`	Naa10	N-alpha-acetyltransferase 10	4	3	1	164.5	Ref	Acetyltransf_1, Acetyltransf_10, Acetyltransf_13, Acetyltransf_15, Acetyltransf_3, Acetyltransf_7, Acetyltransf_9, FR47, Thump_like
`ENSMUSG00000022332`	Khdrbs3	KH domain-containing, RNA-binding, signal transduction-associated protein 3	3	3	1	97.9	Mixed	KH_1, Qua1, STAR_dimer, Sam68-YY
`ENSMUSG00000028772`	Zcchc17	Zinc finger CCHC domain-containing protein 17	4	3	1	19.6	Novel	CDC45, Coilin_N, CpXC, DUF3827, DUF4604, Hid1, Menin, S1, SR-25, zf-CCHC
`ENSMUSG00000022044`	Stmn4	Stathmin-4	4	3	1	560.2	Ref	ATG14, DHR-2_Lobe_C, EF-hand_14, Exonuc_VII_L, FUT8_N_cat, Pex19, Ribonuc_2-5A, Stathmin, UPF0242
`ENSMUSG00000021759`	Plpp1	Phospholipid phosphatase 1	3	3	1	41.1	Novel	PAP2, PAP2_C
`ENSMUSG00000024381`	Bin1	Myc box-dependent-interacting protein 1	3	3	1	90.1	Mixed	BAR, BLOC1_2, Baculo_PEP_C, DUF5667, DUF948, Laminin_II, PTS_IIA, SH3_1, SH3_2, SH3_9, THP2, YtxH
`ENSMUSG00000053453`	Thoc7	THO complex subunit 7 homolog	4	3	1	144.9	Mixed	AATF-Che1, COG2, Com_YlbF, DASH_Dad2, DUF1664, DUF4407, EcoEI_R_C, FapA, Med9, THOC7, Tup_N, UPF0193, V_ATPase_I, ubiquitin
`ENSMUSG00000031536`	Polb	DNA polymerase beta	5	3	1	86.2	Novel	DNA_pol_B_palm, DNA_pol_B_thumb, ExoD, HHH_8, NTP_transf_2
`ENSMUSG00000044477`	Zfand3	AN1-type zinc finger protein 3	3	3	1	15.7	Ref	zf-A20, zf-AN1
`ENSMUSG00000028447`	Dctn3	Dynactin subunit 3	3	3	1	37.2	Mixed	DUF4011, Dynactin_p22, Pox_A_type_inc, Spidroin_MaSp
`ENSMUSG00000031342`	Gpm6b	Neuronal membrane glycoprotein M6-b	5	3	1	37.2	Ref	DUF373, LapA_dom, Myelin_PLP, PspB
`ENSMUSG00000000827`	Tpd52l2	Tumor protein D54	4	3	1	13.7	Mixed	HIP1_clath_bdg, TPD52, VASP_tetra
`ENSMUSG00000060373`	Hnrnpc	Heterogeneous nuclear ribonucleoproteins C1/C2	6	3	1	94.0	Ref	FUT8_N_cat, RRM_1, SKA1, Saf4_Yju2, XhlA, YabA
`ENSMUSG00000027012`	Dync1i2	Cytoplasmic dynein 1 intermediate chain 2	4	3	1	19.6	Ref	ANAPC4_WD40, DUF6465, Dynein_IC2, FYDLN_acid, WD40
`ENSMUSG00000074129`	Rpl13a	Large ribosomal subunit protein uL13	3	3	1	2,046.8	Ref	Ribosomal_L13, SLT_L
`ENSMUSG00000024845`	Tmem134	Transmembrane protein 134	5	3	1	97.9	Ref	TMEM_230_134
`ENSMUSG00000027680`	Fxr1	RNA-binding protein FXR1	3	3	1	9.8	Ref	Agenet, FXMRP1_C_core, FXR_C1, FXR_C3, KH_1, KH_2, KH_4, KH_9, Tudor_2, Tudor_FRX1
`ENSMUSG00000055447`	Cd47	Leukocyte surface antigen CD47	5	3	1	80.3	Ref	CD47, Ig_3, Mpo1-like, TssN, V-set_CD47
`ENSMUSG00000025878`	Uimc1	BRCA1-A complex subunit RAP80	3	3	1	9.8	Ref	LIDHydrolase, RAP80_UIM, Rad50_zn_hook, UBZ_FAAP20, UIM, XAF1_C, zf_UBZ
`ENSMUSG00000045251`	ZNF688	Zinc finger protein 688	4	3	1	15.7	Mixed	FYVE_2, KRAB, OrfB_Zn_ribbon, TFIIS_C, Vps36-NZF-N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-ISL3, zf-LYAR, zf-met, zf_UBZ, zinc_ribbon_9
`ENSMUSG00000033379`	Atp6v0b	V-type proton ATPase 21 kDa proteolipid subunit c''	3	3	1	1,408.3	Ref	ATP-synt_C, G0-G1_switch_2, PIRT
`ENSMUSG00000054766`	Set	Protein SET	3	3	1	17.6	Ref	Mst1_SARAH, NAP, Phage_Mu_Gam
`ENSMUSG00000024617`	CAMK2A	Calcium/calmodulin-dependent protein kinase type II subunit alpha	3	3	1	172.4	Mixed	APH, CaMKII_AD, DUF4440, FTA2, Kdo, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SnoaL_2, SnoaL_3, SnoaL_4
`ENSMUSG00000057506`	Bloc1s2	Biogenesis of lysosome-related organelles complex 1 subunit 2	4	3	1	180.2	Mixed	BLOC1_2, CENP-Q, DUF948, DivIC, Fib_alpha, HEF_HK, NPV_P10, ParB, Uso1_p115_C, XhlA, ZapA
`ENSMUSG00000024227`	—	—	3	3	2	3.9	Novel	GAP1-M, IQ_SEC7_PH, PH, PH_11, PH_8
`ENSMUSG00000031570`	Plpp5	Phospholipid phosphatase 5	3	3	4	25.5	Ref	CoxIIa, PAP2
`ENSMUSG00000063179`	Pstk	L-seryl-tRNA(Sec) kinase	3	3	4	19.6	Novel	KTI12, LMBR1
`ENSMUSG00000020738`	SUMO2	Small ubiquitin-related modifier 2	3	3	4	1,604.2	Ref	Rad60-SLD, ubiquitin
`ENSMUSG00000015488`	Cacfd1	Calcium channel flower homolog	4	3	4	15.7	Mixed	Bacteriocin_IIc, COPI_assoc, Cg6151-P, TMEM72
`ENSMUSG00000029309`	Sparcl1	SPARC-like protein 1	2	2	1	1,282.9	Novel	CdiI_2, EF-hand_1, EF-hand_6, FOLN, Kazal_1, Kazal_2, SPARC_Ca_bdg
`ENSMUSG00000068134`	Zfp120	Zinc finger protein 120	2	2	1	2.0	Ref	Baculo_LEF-11, DUF285, DnaB, KRAB, NCD1, REV1_C, SWIB
`ENSMUSG00000004070`	Hmox2	Heme oxygenase 2	3	2	1	49.0	Mixed	CENP-H, Heme_oxygenase, SsgA
`ENSMUSG00000024462`	Gabbr1	Gamma-aminobutyric acid type B receptor subunit 1	5	2	1	64.6	Mixed	Sushi
`ENSMUSG00000025393`	Atp5f1b	ATP synthase F(1) complex catalytic subunit beta, mitochondrial	2	2	1	278.1	Ref	AAA, AAA_16, AAA_19, ABC_tran, ATP-synt_ab, ATP-synt_ab_N, ATPase, ATPase_2, DUF2075, DUF5832, NACHT, NB-ARC, RsgA_GTPase, T3SS_ATPase_C
`ENSMUSG00000028218`	Cibar1	CBY1-interacting BAR domain-containing protein 1	2	2	1	139.1	Novel	DUF6447, FAM92, FapA, P4Ha_N, Syntaxin-6_N, Vps5
`ENSMUSG00000060038`	Dhps	Deoxyhypusine synthase	2	2	1	84.2	Ref	Carbam_trans_N, DS
`ENSMUSG00000022111`	Uchl3	Ubiquitin carboxyl-terminal hydrolase isozyme L3	2	2	1	170.4	Novel	HpaB, Peptidase_C12
`ENSMUSG00000021071`	Trim9	E3 ubiquitin-protein ligase TRIM9	3	2	1	68.6	Ref	DUF2203, Prok-RING_4, Trm112p, fn3, zf-B_box, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000063931`	Pepd	Xaa-Pro dipeptidase	2	2	1	78.3	Novel	AMP_N, DUF3793, Peptidase_M24
`ENSMUSG00000030980`	Knop1	Lysine-rich nucleolar protein 1	2	2	1	2.0	Ref	DUF5771, SMAP
`ENSMUSG00000000355`	Mcts1	Malignant T-cell-amplified sequence 1	3	2	1	107.7	Novel	DUF1947, Methyltr_RsmF_N, PUA, Pre-PUA
`ENSMUSG00000022800`	Fyttd1	UAP56-interacting factor	2	2	1	35.3	Ref	DUF1583_N, FYTT, RNA_bind, Rpp20
`ENSMUSG00000109865`	Hspa14	Heat shock 70 kDa protein 14	2	2	1	64.6	Novel	Actin, DUF3944, FtsA, HSP70, MreB_Mbl, StbA
`ENSMUSG00000010110`	Stx5	Syntaxin-5	3	2	1	19.6	Ref	Apolipoprotein, BLOC1_2, DUF16, DUF1664, DUF5063, DUF5082, Fib_alpha, IZUMO, LPP, MCPsignal, NPV_P10, SKA1, SNARE, Spectrin, Syntaxin, Syntaxin-5_N, Troponin, Vac_Fusion
`ENSMUSG00000021124`	Vti1b	Vesicle transport through interaction with t-SNAREs homolog 1B	2	2	1	90.1	Ref	4HB_MCP_1, CCDC-167, Cir_Bir_Yir, DASH_Duo1, DUF1759, DUF3376, DUF3958, DUF6245, DUF745, Exonuc_VII_L, Fusion_gly, KxDL, Laminin_II, OmpH, Sec20, Snapin_Pallidin, Syntaxin-6_N, UPF0449, V-SNARE, V-SNARE_C, YqjK
`ENSMUSG00000026767`	Mindy3	Ubiquitin carboxyl-terminal hydrolase MINDY-3	5	2	1	13.7	Mixed	EF-hand_7, MINDY-3_4_CD
`ENSMUSG00000032869`	Psmf1	Proteasome inhibitor PI31 subunit	2	2	1	31.3	Ref	PI31_Prot_C, PI31_Prot_N
`ENSMUSG00000034614`	Pik3ip1	Phosphoinositide-3-kinase-interacting protein 1	2	2	1	41.1	Ref	Kringle, TMEM40
`ENSMUSG00000022749`	Tbc1d23	TBC1 domain family member 23	2	2	1	9.8	Ref	RabGAP-TBC, Rhodanese, TBC1D23_C
`ENSMUSG00000062006`	Rpl34	Large ribosomal subunit protein eL34	3	2	1	86.2	Ref	Ribosomal_L34e
`ENSMUSG00000020849`	YWHAE	14-3-3 protein epsilon	2	2	1	199.8	Novel	14-3-3, Orbi_VP5, Ta0938
`ENSMUSG00000038195`	Rilp	Rab-interacting lysosomal protein	2	2	1	5.9	Ref	DUF6215, GSK-3_bind, Jnk-SapK_ap_N, Macoilin, RILP, V_ATPase_I
`ENSMUSG00000049044`	Rapgef4	Rap guanine nucleotide exchange factor 4	2	2	1	23.5	Ref	DEP, RasGEF, RasGEF_N, cNMP_binding
`ENSMUSG00000039745`	Htatip2	Protein HTATIP2	2	2	1	11.8	Novel	ANF_receptor, Epimerase, NAD_binding_10, NAD_binding_4, Sacchrp_dh_NADP, Semialdhyde_dh
`ENSMUSG00000029433`	Diablo	Diablo IAP-binding mitochondrial protein	2	2	1	33.3	Ref	Hc1, MAGE, Smac_DIABLO
`ENSMUSG00000053253`	Ndfip2	NEDD4 family-interacting protein 2 (Fragment)	3	2	1	33.3	Ref	DUF2370, DUF2614
`ENSMUSG00000007610`	Gtpbp3	5-taurinomethyluridine-[tRNA] synthase subunit GTPB3, mitochondrial	2	2	1	3.9	Ref	AAA_16, AAA_18, AAA_24, AAA_28, ABC_tran, Dynamin_N, FeoB_N, MMR_HSR1, MnmE_helical, Paramyx_P_V_C, RsgA_GTPase, TrmE_N
`ENSMUSG00000039917`	Rhbdd2	Rhomboid domain-containing protein 2	2	2	1	19.6	Ref	DER1, DUF2070, Rhomboid
`ENSMUSG00000054737`	Znf182	Zinc finger protein 182	2	2	1	0.0	Ref	C1_1, C1_4, CpXC, DTC, DUF4934, DUF899, HTH_18, KRAB, LIM, MBD, XPA_N, YhfH, Zn-ribbon_8, Zn_ribbon_SprT, zf-AN1, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-LYAR, zf_UBZ, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000027534`	Snx16	Sorting nexin-16	2	2	1	21.5	Ref	DUF641, HIP1_clath_bdg, LRRFIP, PV-1, PX
`ENSMUSG00000024234`	Mtpap	Poly(A) RNA polymerase, mitochondrial	3	2	1	19.6	Ref	Nrap_D2, PAP_assoc, RL, tRNA_NucTransf2
`ENSMUSG00000024962`	Vegfb	Vascular endothelial growth factor B	4	2	1	152.8	Mixed	PDGF
`ENSMUSG00000037058`	Paip2	Polyadenylate-binding protein-interacting protein 2	3	2	1	280.1	Novel	PAM2
`ENSMUSG00000036748`	Cuedc2	CUE domain-containing protein 2	5	2	1	223.3	Mixed	CUE
`ENSMUSG00000066456`	Hmgn3	High mobility group nucleosome-binding domain-containing protein 3	2	2	1	235.0	Ref	HMG14_17, TT_ORF2, UPF0239
`ENSMUSG00000004267`	Eno2	Gamma-enolase	3	2	1	378.0	Novel	Enolase_C, Enolase_N, IAT_beta, MAAL_C, MR_MLE_C
`ENSMUSG00000032376`	Usp3	Ubiquitin carboxyl-terminal hydrolase 3	2	2	1	7.8	Ref	UCH, UCH_1, zf-UBP
`ENSMUSG00000033526`	Ppip5k1	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1	2	2	1	19.6	Ref	PPIP5K2_N, RimK
`ENSMUSG00000019738`	POLR2I	DNA-directed RNA polymerase II subunit RPB9	2	2	1	70.5	Ref	CpXC, DUF3716, DZR, Ogr_Delta, RNA_POL_M_15KD, TFIIS_C, TF_Zn_Ribbon, Zn-ribbon_8, Zn_Tnp_IS1595, Zn_Tnp_IS91, Zn_ribbon_recom
`ENSMUSG00000031878`	Nae1	NEDD8-activating enzyme E1 regulatory subunit	2	2	1	80.3	Ref	E1_4HB, SEP, ThiF
`ENSMUSG00000061028`	Clasrp	CLK4-associating serine/arginine rich protein	2	2	1	3.9	Ref	DRY_EERY, S-antigen, SDP_N, Trnau1ap
`ENSMUSG00000042182`	Bend6	BEN domain-containing protein 6	2	2	1	25.5	Ref	BEN, CCDC-167, DUF5725, FlaC_arch, K-box, Prefoldin_2, TSC22, bZIP_2
`ENSMUSG00000022210`	Dhrs4	Dehydrogenase/reductase SDR family member 4	2	2	1	31.3	Ref	DDAH_eukar, DFP, Epimerase, F420_oxidored, KR, YjeF_N, adh_short, adh_short_C2
`ENSMUSG00000014767`	Tbp	TATA-box-binding protein	2	2	1	3.9	Ref	ANAPC_CDC26, AcnX_swivel_put, Pox_A6, Presenilin, TBP, TFIIA, Tombus_movement, zf-HIT, zf-MYND
`ENSMUSG00000022702`	Hira	Protein HIRA	2	2	1	7.8	Ref	ANAPC4_WD40, HIRA_B, Hira, Mcl1_mid, NBCH_WD40, STAC2_u1, WD40, WD40_like
`ENSMUSG00000078584`	—	Uncharacterized protein C1orf50 homolog	2	2	1	3.9	Ref	DUF2452, DUF3975, DUF5993, Gallidermin
`ENSMUSG00000002395`	Use1	Vesicle transport protein USE1	5	2	1	258.5	Mixed	Adhes-Ig_like, Amnionless, CCB2_CCB4, Collectrin, DUF5383, MCU, MRP-L28, Orf78, Use1
`ENSMUSG00000027091`	Zc3h15	Zinc finger CCCH domain-containing protein 15	2	2	1	176.3	Ref	DFRP_C, Torus, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf-CCCH_4, zf_CCCH_4
`ENSMUSG00000057236`	RBBP4	Histone-binding protein RBBP4	7	2	1	31.3	Mixed	ANAPC4_WD40, CAF1C_H4-bd, Capsid_NCLDV, NBCH_WD40, WD40, eIF2A
`ENSMUSG00000027282`	Mtch2	Mitochondrial carrier homolog 2	3	2	1	272.3	Ref	Mito_carr
`ENSMUSG00000020925`	Ccdc43	Coiled-coil domain-containing protein 43	2	2	1	49.0	Ref	RepL, SPECT1, TetR_C_28
`ENSMUSG00000027270`	Lamp5	Lysosome-associated membrane glycoprotein 5	2	2	1	477.9	Ref	Chordopox_A13L, Cons_hypoth698, DUF4083, DUF819, Lamp, VAS1_LD
`ENSMUSG00000027406`	Idh3B	Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial	3	2	1	609.2	Novel	AAA_3, Iso_dh
`ENSMUSG00000054863`	TAFA5	Chemokine-like protein TAFA-5	2	2	1	11.8	Ref	BSP_II, CDC45, CENP-B_dimeris, DUF4637, PRR20, Radial_spoke, TAFA, WRNPLPNID
`ENSMUSG00000029922`	Mkrn1	E3 ubiquitin-protein ligase makorin-1	4	2	1	21.5	Mixed	B56, MKRN1_C, Prok-RING_4, Torus, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf-CCCH_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1, zf_CCCH_4
`ENSMUSG00000033342`	Plppr5	Phospholipid phosphatase-related protein type 5	2	2	1	0.0	Ref	DUF6724, Mid2, PAP2, Syndecan, YwiC
`ENSMUSG00000071655`	Ubxn1	UBX domain-containing protein 1	3	2	1	237.0	Ref	CDC45, Presenilin, Selenoprotein_S, UBA, UBX, eIF3_subunit
`ENSMUSG00000030654`	Arl6ip1	ADP-ribosylation factor-like protein 6-interacting protein 1	2	2	1	573.9	Novel	Phage_holin_3_6, Reticulon, TraG_N
`ENSMUSG00000068039`	Tcp1	T-complex protein 1 subunit alpha	3	2	1	78.3	Ref	Cpn60_TCP1
`ENSMUSG00000022837`	Iqcb1	IQ calmodulin-binding motif-containing protein 1	3	2	1	13.7	Novel	Bac_export_1, Condensin2nSMC, DUF1765, IQ
`ENSMUSG00000057672`	Pkn1	Serine/threonine-protein kinase N1	2	2	1	15.7	Ref	7tm_1, ABC1, C2, DUF4231, HR1, Haspin_kinase, Kinase-like, PK_Tyr_Ser-Thr, PL48, Pkinase, Pkinase_C, Uds1
`ENSMUSG00000035863`	Palm	Paralemmin-1	2	2	1	76.4	Ref	FeoB_Cyto, Paralemmin
`ENSMUSG00000056486`	Chn1	N-chimaerin	2	2	1	667.9	Novel	C1_1, FYVE_2, PHD, PHD_2, RhoGAP, zf-RING-like, zf-RING_9
`ENSMUSG00000026019`	Wdr12	Ribosome biogenesis protein WDR12	2	2	1	7.8	Ref	ANAPC4_WD40, DUF6739, NBCH_WD40, NLE, NRDE-2, NUDIX_4, Pap_E4, Pneumo_att_G, RR_TM4-6, Serinc, WD40, Zip
`ENSMUSG00000055078`	Gabra5	Gamma-aminobutyric acid receptor subunit alpha-5	2	2	1	43.1	Ref	CytochromB561_N, FtsX, Neur_chan_LBD, Neur_chan_memb
`ENSMUSG00000040097`	Flywch1	FLYWCH-type zinc finger-containing protein 1	2	2	1	168.4	Novel	CoV_nucleocap, DUF2845, FLYWCH, FLYWCH_N, FLYWCH_u, GspH, NADH_oxidored, SDP_N, ScfRs, YdfZ, Zn_ribbon_recom
`ENSMUSG00000037936`	Scarb1	Scavenger receptor class B member 1	2	2	1	29.4	Ref	CD36, PorA, TMEM51
`ENSMUSG00000038880`	Mrps34	Small ribosomal subunit protein mS34	2	2	1	213.5	Ref	MRP-S34, NDK, PSI_PsaJ, Peptidase_S77
`ENSMUSG00000024949`	Sf1	Splicing factor 1	3	2	1	41.1	Ref	KH_1, KH_5, SF1-HH, zf-CCHC, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000022540`	Rogdi	Protein rogdi homolog	2	2	1	282.1	Novel	Hemopexin, Rogdi_lz
`ENSMUSG00000033287`	KCTD17	BTB/POZ domain-containing protein KCTD17	2	2	1	50.9	Novel	BTB, BTB_2, Peptidase_M90, SAM_LFY
`ENSMUSG00000028647`	Mycbp	c-Myc-binding protein	2	2	1	11.8	Ref	CENP-F_N, TSC22, ZapB
`ENSMUSG00000038876`	Echdc1	Ethylmalonyl-CoA decarboxylase	3	2	1	84.2	Ref	ECH_1, ECH_2, Prok-RING_4, Sp-DndD, WWE, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000063802`	Hspbp1	Hsp70-binding protein 1	4	2	1	205.7	Novel	Arm, CLIP_SPH_mas, Fes1, HEAT, HEAT_EZ, PPTA, Rcd1
`ENSMUSG00000024853`	Sf3b2	Splicing factor 3B subunit 2	2	2	1	96.0	Ref	DUF382, PSP, SAP, SAP_new25
`ENSMUSG00000036225`	KCTD1	BTB/POZ domain-containing protein KCTD1	3	2	1	35.3	Mixed	BTB_2
`ENSMUSG00000063445`	Nmral1	NmrA-like family domain-containing protein 1	2	2	1	49.0	Novel	3Beta_HSD, BRK, Epimerase, F420_oxidored, HTH_3, Helo_like_N, KR, NAD_binding_10, NmrA, Semialdhyde_dh, Shikimate_DH, TrkA_N, adh_short
`ENSMUSG00000002058`	Unc119	Protein unc-119 homolog A	2	2	1	9.8	Ref	GMP_PDE_delta, IIGP, Trypan_PARP
`ENSMUSG00000037032`	Apbb1	Amyloid beta precursor protein binding family B member 1	5	2	1	417.2	Ref	PID, PID_2, PTB, WW
`ENSMUSG00000068329`	Htra2	Serine protease HTRA2, mitochondrial	3	2	1	54.8	Mixed	Peptidase_S46, Trypsin, Trypsin_2
`ENSMUSG00000030086`	Chchd6	MICOS complex subunit Mic25	2	2	1	536.7	Ref	ATG14, Activator-TraM, CHCH, DUF1690, DUF3150, DUF4337, DUF6462, Exonuc_VII_L, FapA, IZUMO, MIC19_MIC25, NDUF_B7, hDGE_amylase
`ENSMUSG00000038482`	Tfdp1	Transcription factor Dp-1	3	2	1	13.7	Ref	DP, E2F_TDP
`ENSMUSG00000022721`	Trmt2a	tRNA (uracil-5-)-methyltransferase homolog A	2	2	1	11.8	Ref	AviRa, CMAS, Cons_hypoth95, MTS, MetW, Methyltransf_11, Methyltransf_12, Methyltransf_15, Methyltransf_23, Methyltransf_25, Methyltransf_31, Methyltransf_32, Methyltransf_4, N6_N4_Mtase, NodS, PCMT, PrmA, RRM_1, TPMT, Ubie_methyltran, tRNA_U5-meth_tr
`ENSMUSG00000022552`	Sharpin	Sharpin	2	2	1	68.6	Novel	Emfourin, Sharpin_PH, ubiquitin, zf-RanBP
`ENSMUSG00000036775`	Decr2	Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]	2	2	1	7.8	Ref	DUF4675, F420_oxidored, KR, adh_short, adh_short_C2
`ENSMUSG00000094936`	Rbm4b	RNA-binding protein 4B	2	2	1	13.7	Ref	Calcipressin, CompInhib_SCIN, DbpA, Nup35_RRM_2, RNA_bind, RRM_1, RRM_5, RRM_7, RRM_occluded, RSD-2, zf-CCHC, zf-CCHC_2, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000021493`	PDLIM7	PDZ and LIM domain protein 7	3	2	1	121.4	Novel	DUF4749, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Rv0078B
`ENSMUSG00000020585`	Laptm4a	Lysosomal-associated transmembrane protein 4A	2	2	1	570.0	Novel	Circovir2_Orf4, DUF4728, Mtp
`ENSMUSG00000030774`	Pak1	Serine/threonine-protein kinase PAK 1	3	2	1	82.3	Novel	ABC1, APH, DSHCT, DUF3741, Kdo, Kinase-like, PBD, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal
`ENSMUSG00000028466`	Creb3	Cyclic AMP-responsive element-binding protein 3	2	2	1	58.8	Novel	DUF2721, DUF6262, GIT_CC, ZapB, bZIP_1, bZIP_2, bZIP_Maf
`ENSMUSG00000060743`	His3.3A	Histone H3.3A	2	2	1	164.5	Ref	CBFD_NFYB_HMF, CENP-S, CENP-T_C, GHL13, Histone, PAF
`ENSMUSG00000064264`	Znf428	Zinc finger protein 428	2	2	1	90.1	Ref	PIEZO, zf-C2H2_4
`ENSMUSG00000004567`	Mcoln1	Mucolipin-1	2	2	1	50.9	Ref	DUF1866, DUF389, Ion_trans, PKD_channel
`ENSMUSG00000039069`	MTG2	Mitochondrial ribosome-associated GTPase 2	2	2	1	3.9	Novel	Arf, Dynamin_N, FeoB_N, GTP1_OBG, MMR_HSR1, Ras, cobW
`ENSMUSG00000018398`	Septin8	Septin-8	3	2	1	205.7	Ref	AAA_16, AAA_22, AAA_23, AAA_24, AAA_29, AAA_7, ABC_tran, AIG1, ATG14, ATP_bind_1, DUF5906, DUF87, Dynamin_N, GTP_EFTU, IMD, MMR_HSR1, RHD3_GTPase, RNA_helicase, Ras, Roc, RsgA_GTPase, Septin, T2SSE
`ENSMUSG00000030605`	Mfge8	Lactadherin	2	2	1	274.2	Ref	EGF, EGF_2, F5_F8_type_C, Laminin_EGF, Muskelin_N, cEGF, hEGF
`ENSMUSG00000037196`	Pacrg	Parkin coregulated gene protein homolog	2	2	1	17.6	Novel	DCB, ParcG, Peptidase_C107, RNA_pol_Rpb2_2
`ENSMUSG00000038302`	Afg1l	AFG1-like ATPase	2	2	1	3.9	Novel	AAA, AAA_16, AAA_22, AFG1_ATPase, Bac_DnaA, DUF2075, ResIII, UvrD-helicase
`ENSMUSG00000006476`	Nsmf	NMDA receptor synaptonuclear signaling and neuronal migration factor	2	2	1	47.0	Mixed	DUF4347, IQ
`ENSMUSG00000028545`	Bend5	BEN domain-containing protein 5	2	2	1	11.8	Ref	AAA_23, BEN, DASH_Spc34, DUF349, DUF5917, DUF6594, Docking, LMBR1, OmpH, PIN_12, Sec8_exocyst, Sipho_Gp157, T4SS, TSNAXIP1_N
`ENSMUSG00000048967`	Yjefn3	YjeF N-terminal domain-containing protein 3	2	2	1	29.4	Novel	DUF4381, GRIM-19, Planc_extracel, YjeF_N
`ENSMUSG00000027805`	PFN2	Profilin-2	2	2	1	534.7	Ref	DUF2076, DUF4106, Profilin
`ENSMUSG00000053119`	Chmp3	Charged multivesicular body protein 3	2	2	1	56.8	Ref	DUF3915, PSD4, PYC_OADA, SPOUT_MTase, Snf7
`ENSMUSG00000040746`	Rnf167	E3 ubiquitin-protein ligase RNF167	2	2	1	111.6	Novel	CTU2, FAM176, FANCL_C, PA, PHD, Prok-RING_1, Prok-RING_4, RINGv, RecR, zf-ANAPC11, zf-C3H2C3, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING-like, zf-RING_11, zf-RING_15, zf-RING_16, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-UDP, zf-rbx1
`ENSMUSG00000025508`	Rplp2	Large ribosomal subunit protein P2	3	2	1	111.6	Ref	EF-hand_14, NolX, RelA_AH_RIS, Ribosomal_60s, TFIID_30kDa
`ENSMUSG00000037788`	Vopp1	WW domain binding protein VOPP1	2	2	1	60.7	Ref	Conotoxin_I2, FixQ, TMEM52, WBP-1, zf-His_Me_endon
`ENSMUSG00000040390`	Map3k10	Mitogen-activated protein kinase kinase kinase 10	2	2	1	33.3	Novel	ABC1, Epsilon_antitox, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SH3_1, SH3_2, SH3_9
`ENSMUSG00000010936`	Vac14	Protein VAC14 homolog	2	2	1	15.7	Ref	Cnd1, HEAT, HEAT_2, HEAT_EZ, MMS22L_C, TAF6_C, Vac14_Fab1_bd, Vac14_Fig4_bd
`ENSMUSG00000013155`	Enkd1	Enkurin domain-containing protein 1	2	2	1	5.9	Ref	Chibby, Enkurin
`ENSMUSG00000029610`	Aimp2	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2	2	2	1	101.9	Ref	AIMP2_LysRS_bd, GST_C, GST_C_2, GST_C_3, GST_C_6, GTP-bdg_M, Thioredoxin_16
`ENSMUSG00000047342`	ZNF286A	Zinc finger protein 286A	2	2	1	17.6	Ref	C1_4, DNA_RNApol_7kD, DUF5830, DUF6076, DZR, HVO_2753_ZBP, HalOD2, KRAB, LIM, OSTMP1, TFIIS_C, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-met, zf-trcl, zf_UBZ, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000025264`	Tsr2	Pre-rRNA-processing protein TSR2 homolog	2	2	1	21.5	Ref	DUF6279, LPD22, WGG
`ENSMUSG00000030126`	Tmcc1	Transmembrane and coiled-coil domains protein 1	2	2	1	17.6	Ref	C2orf69, CCDC28, CbiA, DUF1664, DUF334, DUF4131, DUF6264, Exonuc_VII_L, LMBR1, Latarcin, OmpH, Rootletin, SYMPK_PTA1_N, TMPIT, Tmemb_cc2, Tup_N, Vma12
`ENSMUSG00000032305`	Fam219b	Protein FAM219B	2	2	1	2.0	Ref	DUF6749, FAM219A
`ENSMUSG00000024118`	Tedc2	Tubulin epsilon and delta complex protein 2	2	2	1	7.8	Ref	DUF1633, DUF4693
`ENSMUSG00000024797`	Vps51	Vacuolar protein sorting-associated protein 51 homolog	2	2	1	27.4	Ref	COG2, COG5, DUF3829, Dor1, GIT_CC, Occludin_ELL, Sec5, TMTC_DUF1736, Vps51, Vps53_N, Vps54_N, Zw10
`ENSMUSG00000075704`	Txnrd2	Thioredoxin reductase 2, mitochondrial	3	2	1	33.3	Mixed	AlaDh_PNT_C, DAO, FAD_binding_2, FAD_oxidored, GIDA, HI0933_like, NAD_binding_8, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Pyr_redox_dim, Thi4
`ENSMUSG00000008305`	Tle1	Transducin-like enhancer protein 1	2	2	1	15.7	Ref	ANAPC4_WD40, Cytochrom_D1, HALZ, NBCH_WD40, Nup160, Ribosomal_S18, Spc24, TLE_N, Transketolase_N, WD40, WD40_like
`ENSMUSG00000052915`	Msl1	Male-specific lethal 1 homolog	2	2	1	66.6	Ref	ATG14, DUF3287, DivIC, Herpes_UL6, KLRAQ, Laminin_I, MSL1_dimer, PEHE
`ENSMUSG00000027333`	Smox	Spermine oxidase	3	2	1	25.5	Ref	3HCDH_N, AlaDh_PNT_C, Amino_oxidase, DAO, FAD_binding_2, FAD_binding_3, FAD_oxidored, FMO-like, GIDA, HI0933_like, Lycopene_cycl, MCRA, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Shikimate_DH, Thi4
`ENSMUSG00000026171`	Rnf25	E3 ubiquitin-protein ligase RNF25	2	2	1	31.3	Ref	CpXC, DUF3785, Papo_T_antigen, RWD, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000037601`	Nme1	Nucleoside diphosphate kinase A	2	2	1	137.1	Novel	DUF6486, NDK
`ENSMUSG00000026575`	Nme7	Nucleoside diphosphate kinase homolog 7	2	2	1	5.9	Novel	Arg_repressor, DM10_dom, NDK
`ENSMUSG00000026245`	Farsb	Phenylalanine--tRNA ligase beta subunit	3	2	1	45.0	Ref	B3_4, B5, EAD7, PhetRS_B1, tRNA_synthFbeta
`ENSMUSG00000014232`	Cluap1	Clusterin-associated protein 1	2	2	1	39.2	Novel	ATG14, BUD22, CENP-B_dimeris, Cluap1, Jnk-SapK_ap_N, PBP1_TM, RasGAP_C, Tweety
`ENSMUSG00000049680`	Urgcp	Up-regulator of cell proliferation	2	2	1	3.9	Ref	AAA_16, ARPC4, MMR_HSR1, PXA, RHD3_GTPase
`ENSMUSG00000026888`	Grb14	Growth factor receptor-bound protein 14	2	2	1	88.1	Novel	BPS, PH, PH_20, RA, SH2
`ENSMUSG00000079557`	Marchf2	E3 ubiquitin-protein ligase MARCHF2	3	2	1	84.2	Mixed	Baculo_IE-1, RINGv, Vpu, zf-C3HC4, zf-RING_2, zf-rbx1
`ENSMUSG00000037740`	Mrps26	Small ribosomal subunit protein mS26	2	2	1	103.8	Ref	DUF1127, MAJIN, MRP-S26, PBP_sp32
`ENSMUSG00000024055`	Cyp4f6	Cytochrome P450 4F6	2	2	1	11.8	Ref	Diacid_rec, TetR_C_28, p450
`ENSMUSG00000076432`	Ywhaq	14-3-3 protein theta	2	2	1	158.7	Ref	14-3-3, AAA_15, FliM, XhlA
`ENSMUSG00000025786`	Zdhhc3	Palmitoyltransferase ZDHHC3	2	2	1	52.9	Ref	DHHC, DUF3615, DUF6404, DUF898, GPDPase_memb
`ENSMUSG00000017404`	RPL19	Large ribosomal subunit protein eL19	3	2	1	2,013.5	Novel	Ribosomal_L19e
`ENSMUSG00000001056`	Nhp2	H/ACA ribonucleoprotein complex subunit 2	2	2	1	84.2	Ref	ETC_C1_NDUFA5, Phage_RpbA, Ribosomal_L31e, Ribosomal_L7Ae, UPF0203, zinc_ribbon_12
`ENSMUSG00000060126`	Tpt1	Translationally-controlled tumor protein	3	2	1	205.7	Novel	DUF6613, TCTP
`ENSMUSG00000045007`	Tubg2	Tubulin gamma-2 chain	2	2	1	117.5	Novel	Misat_Tub_SegII, Sp-DndD, Tubulin, Tubulin_2, Tubulin_C
`ENSMUSG00000050711`	Scg2	Secretogranin-2	2	2	1	66.6	Ref	Granin, IF2_N, eIF3_N
`ENSMUSG00000029713`	GNB2	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	2	2	1	348.6	Ref	ANAPC4_WD40, CaM_bind, Cytochrom_D1, Ge1_WD40, NBCH_WD40, WD40, WD40_like
`ENSMUSG00000001844`	Zdhhc4	Palmitoyltransferase ZDHHC4	2	2	1	27.4	Novel	DHHC, DUF417
`ENSMUSG00000027206`	COPS2	COP9 signalosome complex subunit 2	2	2	1	60.7	Ref	FliS, PCI, TPR_1, TPR_2, TPR_7, YozE_SAM_like
`ENSMUSG00000027502`	Rtf2	Replication termination factor 2	2	2	1	68.6	Novel	ELH, Rtf2, zf-NOSIP
`ENSMUSG00000020308`	Tpgs1	Tubulin polyglutamylase complex subunit 1	2	2	1	213.5	Novel	DUF2267, Dpy-30, RIIa
`ENSMUSG00000048330`	Ric3	Protein RIC-3	3	2	1	9.8	Ref	CHASE3, RIC3
`ENSMUSG00000031622`	Sin3b	Paired amphipathic helix protein Sin3b	3	2	1	103.8	Ref	PAH
`ENSMUSG00000067150`	Xpo5	Exportin-5	2	2	1	23.5	Ref	Exportin-5, IBN_N, IMD, UCH, Xpo1
`ENSMUSG00000028152`	TSPAN5	Tetraspanin-5	2	2	1	144.9	Ref	DUF4378, Maf_N, Phage_holin_3_6, Tetraspanin
`ENSMUSG00000061086`	Myl4	Myosin light chain 4	4	2	1	144.9	Novel	EF-hand_1, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9
`ENSMUSG00000024194`	Cuta	Protein CutA	9	2	1	219.4	Mixed	CutA1, DAHP_synth_1
`ENSMUSG00000027108`	Ola1	Obg-like ATPase 1	2	2	1	101.9	Ref	AAA_18, ABC_tran, Dynamin_N, FeoB_N, MMR_HSR1, MeaB, Roc, TGS, YchF-GTPase_C
`ENSMUSG00000028034`	Fubp1	Far upstream element-binding protein 1	3	2	1	31.3	Ref	DUF1897, KH_1, KH_2, KH_4, KH_5, NAD_kinase_C, Secretin_N
`ENSMUSG00000028945`	Rheb	GTP-binding protein Rheb	2	2	1	70.5	Novel	AAA_14, Arf, DUF1842, GTP_EFTU, MMR_HSR1, Ras, RhoGAP_pG1_pG2, Roc, RsgA_GTPase, SRPRB
`ENSMUSG00000030304`	Ergic2	Endoplasmic reticulum-Golgi intermediate compartment protein 2	2	2	1	23.5	Ref	COPIIcoated_ERV, ERGIC_N
`ENSMUSG00000032965`	Ift57	Intraflagellar transport protein 57 homolog	2	2	1	33.3	Ref	ATG14, DASH_Dad1, DUF1664, DUF4407, DUF745, Fez1, HAD_2, HrpB7, IFT57, KxDL, Laminin_II, MCPsignal, Mod_r, MscS_porin, VASP_tetra, WXG100
`ENSMUSG00000042389`	Tsen2	tRNA-splicing endonuclease subunit Sen2	3	2	1	3.9	Mixed	FLYWCH_u, tRNA_int_endo, tRNA_int_endo_N
`ENSMUSG00000030613`	Ccdc90b	Coiled-coil domain-containing protein 90B, mitochondrial	2	2	1	17.6	Ref	Activator-TraM, CCDC90-like, DUF3509, Lipoprotein_7, MHC_I_2, PRKG1_interact, Vac_Fusion, YabA
`ENSMUSG00000039278`	Pcsk1n	ProSAAS	2	2	1	88.1	Novel	FUSC, ProSAAS
`ENSMUSG00000028657`	Ppt1	Palmitoyl-protein thioesterase 1	2	2	1	33.3	Ref	Abhydrolase_1, Palm_thioest, U71
`ENSMUSG00000031358`	Msl3	MSL complex subunit 3	2	2	1	3.9	Ref	Chromo, MRG, Pih1_fungal_CS, Tudor-knot
`ENSMUSG00000002428`	Hltf	Helicase-like transcription factor	2	2	1	9.8	Ref	AAA_34, HIRAN, Helicase_C, Prok-RING_4, ResIII, SNF2-rel_dom, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Nse, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX
`ENSMUSG00000039419`	Cntnap2	Contactin-associated protein-like 2	2	2	1	19.6	Ref	Adeno_E3_CR2, COLFI, EGF, F5_F8_type_C, Laminin_G_1, Laminin_G_2, Laminin_G_3, MAP17, MARVEL, Syndecan, Tmemb_9, UstYa, hEGF
`ENSMUSG00000029601`	Iqcd	Dynein regulatory complex protein 10	2	2	1	2.0	Novel	DUF4604, DUF6739, IQ, Nucleoporin_FG2, Phlebovirus_NSM, Wound_ind
`ENSMUSG00000022791`	Tnk2	Activated CDC42 kinase 1	2	2	1	23.5	Novel	HTH_8, Inhibitor_Mig-6, UBA, UBA_4
`ENSMUSG00000030663`	Smap	Small acidic protein	2	2	1	311.4	Novel	BUD22, CDC45, Nop14, RNA_polI_A34, SMAP
`ENSMUSG00000028862`	Map3k6	Mitogen-activated protein kinase kinase kinase 6	2	2	1	25.5	Ref	ASK_PH, DRHyd-ASK, Glu-tRNAGln, HisK-N-like, MAP3K_TRAF_bd, NTP_transf_5, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SAM_KSR1, TPR-S, YrbL-PhoP_reg
`ENSMUSG00000035953`	Pip4p1	Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase	3	2	1	25.5	Ref	FlhC, Herpes_US9, Tmemb_55A, zinc_ribbon_12
`ENSMUSG00000035776`	Cd99l2	CD99 antigen-like protein 2	2	2	1	5.9	Ref	ACT_7, CD99L2, Podoplanin, Ribosomal_L23eN
`ENSMUSG00000055553`	Kxd1	KxDL motif-containing protein 1	3	2	1	113.6	Novel	KxDL, TolA_bind_tri
`ENSMUSG00000031865`	Dctn1	Dynactin subunit 1	2	2	1	56.8	Novel	ADIP, AIP3, ATG14, ATG17_like, ATG7_N, Baculo_PEP_C, CAP_GLY, DUF2443, DUF2935, DUF4172, Dynactin, Fez1, Filament, HMMR_N, HsbA, JIP_LZII, MCC-bdg_PDZ, NPV_P10, Pox_A_type_inc, Spike_torovirin, Ufd2P_core
`ENSMUSG00000025474`	Tubgcp2	Gamma-tubulin complex component 2	2	2	1	2.0	Novel	GCP_C_terminal, zf-Tim10_DDP
`ENSMUSG00000050310`	Rictor	Rapamycin-insensitive companion of mTOR	2	2	1	23.5	Ref	7TM_GPCR_Srbc, DUF3385, RICTOR_N
`ENSMUSG00000016503`	Gtf3a	Transcription factor IIIA	2	2	1	68.6	Ref	C1_2, CCAP, CHORD, FOXP-CC, Ribosomal_S14, Spt46, zf-C2H2, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_aberr, zf-C2H2_jaz, zf-C2HC_2, zf-C2HE, zf-Di19, zf-H2C2_2, zf-H2C2_5, zf-met, zf_Hakai, zf_ZIC
`ENSMUSG00000022634`	Yaf2	YY1-associated factor 2	2	2	1	43.1	Ref	C1_2, Ephrin_rec_like, PROCN, RAP1, WTX, YAF2_RYBP, zf-RanBP
`ENSMUSG00000031516`	Dctn6	Dynactin subunit 6	2	2	1	334.9	Novel	Fucokinase, Hexapep
`ENSMUSG00000042354`	Gnl3	Guanine nucleotide-binding protein-like 3	2	2	1	17.6	Ref	BRD4_CDT, Dynamin_N, FeoB_N, GN3L_Grn1, GTP_EFTU, MMR_HSR1, MeaB, RsgA_GTPase
`ENSMUSG00000041417`	Pik3r1	Phosphatidylinositol 3-kinase regulatory subunit alpha	2	2	1	43.1	Ref	APG6_N, BRE1, Bap31_Bap29_C, DUF1664, DUF2203, DUF412, DUF4806, LMBR1, Laminin_I, Mod_r, Occludin_ELL, Orf78, PI3K_P85_iSH2, PI_PP_I, PRT_C, RhoGAP, SAYSvFN, SH2, SH3_1, SH3_2, SH3_9, Seryl_tRNA_N, Transcrip_act, VirB3
`ENSMUSG00000031916`	Cog8	Conserved oligomeric Golgi complex subunit 8	2	2	1	5.9	Ref	DUF4279, Dor1, Pep_deformylase, Vps51
`ENSMUSG00000032172`	OLFM2	Noelin-2	2	2	1	146.9	Ref	ABC_tran_CTD, CLZ, DUF1664, Ephrin, Helo_like_N, Matrilin_ccoil, Noelin-1, OLF, PepSY_2
`ENSMUSG00000021996`	Esd	S-formylglutathione hydrolase	2	2	1	90.1	Ref	AXE1, Abhydrolase_2, Abhydrolase_3, BD-FAE, Chlorophyllase, Chlorophyllase2, Esterase, Esterase_PHB, Hydrolase_4, Lipase_3, Peptidase_S9, Shadoo
`ENSMUSG00000020175`	Rab36	Ras-related protein Rab-36	3	2	1	9.8	Ref	AAA_14, Arf, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB
`ENSMUSG00000021546`	HNRNPK	Heterogeneous nuclear ribonucleoprotein K	3	2	1	68.6	Ref	DJ-1_PfpI, DUF719, KH_1, KH_2, KH_4, KH_5, MOEP19, ROKNT
`ENSMUSG00000030401`	Rtn2	Reticulon-2	2	2	1	49.0	Ref	Babuvirus_MP, DUF592, Reticulon, mono-CXXC
`ENSMUSG00000050174`	Nudt6	Nucleoside diphosphate-linked moiety X motif 6	3	2	1	5.9	Novel	NUDIX, NUDIX_4, Nudix_hydro
`ENSMUSG00000036214`	Polr1has	RNA polymerase I subunit H	2	2	1	13.7	Ref	C4, DUF4131, DUF5520, PRIMA1
`ENSMUSG00000026614`	Slc30a10	Calcium/manganese antiporter SLC30A10	2	2	1	0.0	Ref	Cation_efflux, DUF2530, HTH_SUN2, Holin_BlyA
`ENSMUSG00000063457`	RPS15	Small ribosomal subunit protein uS19	2	2	1	1,400.5	Ref	LCAT, MAP65_ASE1, Prok_Ub, Ribosomal_S19
`ENSMUSG00000059456`	Ptk2b	Protein-tyrosine kinase 2-beta	2	2	1	190.0	Ref	FERM_F2, FERM_M, FERM_N_2, Focal_AT, Kinase-like, NTF3_N, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000057531`	Dtnbp1	Dysbindin	3	2	1	60.7	Novel	Dysbindin, POTRA
`ENSMUSG00000041278`	Ttc1	Tetratricopeptide repeat protein 1	2	2	1	152.8	Ref	MG2, RPN7, SOAR, TPR_1, TPR_11, TPR_12, TPR_14, TPR_15, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9, eIF3_N
`ENSMUSG00000022103`	Gfra2	GDNF family receptor alpha-2	2	2	1	86.2	Novel	Apo-CIII, GDNF, HD-ZIP_N
`ENSMUSG00000039615`	Stub1	E3 ubiquitin-protein ligase CHIP	3	2	1	50.9	Ref	ANAPC3, CHIP_TPR_N, NYD-SP12_N, SHNi-TPR, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000039660`	Spout1	28S rRNA (uridine-N(3))-methyltransferase	2	2	1	31.3	Ref	AP1AR, DUF2514, Methyltrn_RNA_3
`ENSMUSG00000052151`	Plpp2	Phospholipid phosphatase 2	2	2	1	3.9	Ref	DUF2976, LrgB, MNHE, PAP2, PAP2_C, TraL, UbiA, Virul_fac_BrkB
`ENSMUSG00000042675`	YPEL3	Protein yippee-like 3	5	2	1	381.9	Mixed	RIG-I_C-RD, Yippee-Mis18
`ENSMUSG00000030990`	Pgap2	Acyltransferase PGAP2	2	2	1	11.8	Ref	AA_permease_2, Frag1, TMEM138, YtpI
`ENSMUSG00000041112`	Elmo1	Engulfment and cell motility protein 1	2	2	1	152.8	Ref	ELMO_ARM, ELMO_CED12, FERM_N, Imm42, Nup96, PH_12, ZFYVE21_C
`ENSMUSG00000000560`	Gabra2	Gamma-aminobutyric acid receptor subunit alpha-2	2	2	1	7.8	Ref	CytochromB561_N, DUF4985, Neur_chan_LBD, Neur_chan_memb
`ENSMUSG00000029319`	Coq2	4-hydroxybenzoate polyprenyltransferase, mitochondrial	2	2	1	47.0	Novel	DUF2798, UbiA
`ENSMUSG00000029131`	Dnajb6	DnaJ homolog subfamily B member 6	2	2	1	427.0	Ref	DnaJ, eIF3_subunit
`ENSMUSG00000014195`	Dnajc7	DnaJ homolog subfamily C member 7	2	2	1	217.4	Ref	ANAPC3, Alkyl_sulf_dimr, BTAD, CCER1, DnaJ, IspA, MIT, NFACT-R_1, RPN7, RPT, Rapsyn_N, SHNi-TPR, Sel1, TPR-S, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_3, TPR_4, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000039217`	Il18	Interleukin-18	2	2	1	5.9	Novel	DUF273, IL1, NCA2
`ENSMUSG00000031728`	Znf821	Zinc finger protein 821	2	2	1	5.9	Ref	CDC45, CPSF73-100_C, DUF5679, DUF5810, DUF6011, DZR, HMBD, Rad50_zn_hook, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_9, zf-C2H2_jaz, zf-Di19, zf-H2C2_2, zf-HYPF, zf-met
`ENSMUSG00000033106`	Slc7a6os	Probable RNA polymerase II nuclear localization protein SLC7A6OS	2	2	1	9.8	Novel	Iwr1, Nop14
`ENSMUSG00000040048`	Ndufb10	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10	2	2	1	785.4	Ref	DUF3498, DUF6040, GCR1_C, NDUFB10
`ENSMUSG00000043445`	Pgp	Glycerol-3-phosphate phosphatase	2	2	1	70.5	Ref	Asp_Glu_race, HAD_2, Hydrolase, Hydrolase_6, Hydrolase_like, PGP_phosphatase
`ENSMUSG00000029053`	Prkcz	Protein kinase C zeta type	2	2	1	84.2	Ref	ABC1, APH, C1_1, C1_2, DZR, FTA2, Haspin_kinase, Kdo, Kinase-like, PB1, PK_Tyr_Ser-Thr, Pkinase, Pkinase_C
`ENSMUSG00000014602`	Kif1a	Kinesin-like protein KIF1A	2	2	1	333.0	Ref	DUF3694, FHA, KIF1B, Kinesin, Kinesin_assoc, Microtub_bd, PH, PH_11, Pox_Ag35, Yop-YscD_cpl
`ENSMUSG00000061702`	Tmem91	Transmembrane protein 91	2	2	1	19.6	Novel	AWPM-19, CD225
`ENSMUSG00000027551`	Zfp64	Zinc finger protein 64	2	2	1	19.6	Ref	BolA, Peptidase_C74, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_3rep, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-H2C2_5, zf-met
`ENSMUSG00000030337`	Vamp1	Vesicle-associated membrane protein 1	2	2	1	60.7	Ref	CD34_antigen, DUF4736, MCU, Orf78, PRIMA1, SNARE, Synaptobrevin
`ENSMUSG00000032182`	Yipf2	Protein YIPF2	3	2	1	5.9	Novel	CAT_RBD, DUF2070, Sec62, Yip1
`ENSMUSG00000031078`	Cttn	Src substrate cortactin	2	2	1	11.8	Novel	CCDC47, DUF3602, HS1_rep, MCU, PV-1, SH3_1, SH3_2, SH3_9, TAF4, TMEM210
`ENSMUSG00000035357`	Pdzrn3	E3 ubiquitin-protein ligase PDZRN3	2	2	1	17.6	Ref	PDZ, PDZ_2, PDZ_6, Peptidase_M50, T2SSppdC, Tricorn_PDZ
`ENSMUSG00000038390`	Gpr162	Probable G-protein coupled receptor 162	2	2	1	31.3	Ref	7TM_GPCR_Srv, 7TM_GPCR_Srx, 7tm_1, PetG, Srg
`ENSMUSG00000020792`	Exoc7	Exocyst complex component 7	4	2	1	7.8	Mixed	COG5, Exo70, RolB_RolC, Sec34, Vps52
`ENSMUSG00000032673`	Prorsd1	Prolyl-tRNA synthetase associated domain-containing protein 1	2	2	1	9.8	Ref	DpnI_C, PRORP, SinI, tRNA_edit
`ENSMUSG00000035835`	Plppr3	Phospholipid phosphatase-related protein type 3	2	2	1	19.6	Ref	BSP_II, CENP-B_dimeris, DUF4746, PAP2, RNA_pol_3_Rpc31
`ENSMUSG00000029125`	Stx18	Syntaxin-18	3	2	1	17.6	Ref	DUF6279, Phage_CP76, Syntaxin-18_N, TipAS
`ENSMUSG00000021276`	Cinp	Cyclin-dependent kinase 2-interacting protein	2	2	1	31.3	Ref	DUF5089, HAUS-augmin3, Tmemb_cc2, WDCP
`ENSMUSG00000020335`	Znf354b	Zinc finger protein 354B	2	2	1	5.9	Novel	BolA, C1_4, HypA, KRAB, LIM, Ribosomal_L17, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-H2C2_5, zf-met, zf_C2H2_13, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000020755`	Sap30bp	SAP30-binding protein	2	2	1	21.5	Novel	CCDC84, HCNGP
`ENSMUSG00000022340`	Sybu	Syntabulin	2	2	1	3.9	Novel	BST2, CALCOCO1, CC2-LZ, DUF16, DUF1635, DUF4200, DUF641, DUF745, Exonuc_VII_L, FAM76, FPP, FapA, Fez1, HR1, PKcGMP_CC, Sec2p, Sec8_exocyst, Syntaphilin, TMPIT, TPR_MLP1_2, UPF0242, UPF0561
`ENSMUSG00000030922`	Lyrm1	LYR motif-containing protein 1	2	2	1	15.7	Ref	Complex1_LYR, Complex1_LYR_2, DUF948, SKA2
`ENSMUSG00000027706`	Sec62	Translocation protein SEC62	2	2	1	27.4	Novel	BUD22, CIS_TMP, DEP, Dsh_C, EOS1, RR_TM4-6, SLC12, Sec62, YhhN
`ENSMUSG00000020923`	Ubtf	Nucleolar transcription factor 1	2	2	1	56.8	Ref	Chlor_dismutase, DUF4473, HMG_box, HMG_box_2, HMG_box_5, Ins134_P3_kin_N, NDUF_C2, Protamine_like
`ENSMUSG00000015806`	Qdpr	Dihydropteridine reductase	3	2	1	693.4	Ref	Peptidase_S66, adh_short, adh_short_C2
`ENSMUSG00000003549`	Ercc1	DNA excision repair protein ERCC-1	2	2	1	82.3	Ref	HHH, HHH_2, HHH_5, Rad10, UPF0758_N
`ENSMUSG00000021532`	Fastkd3	FAST kinase domain-containing protein 3, mitochondrial	3	2	1	9.8	Novel	FAST_1, FAST_2, RAP
`ENSMUSG00000021750`	Fam107a	Actin-associated protein FAM107A	6	2	1	17.6	Novel	DUF1151, KIAA1328, RNase_Y_N
`ENSMUSG00000060073`	Psma3	Proteasome subunit alpha type-3	4	2	1	658.1	Ref	Arm-DNA-bind_3, Proteasome, Proteasome_A_N
`ENSMUSG00000026418`	Tnni1	Troponin I, slow skeletal muscle	2	2	1	2.0	Novel	Apis_Csd, Atrophin-1, Auts2, BES1_N, DUF2951, DUF3915, FAM27, PDCD7, Troponin, XkdW
`ENSMUSG00000031897`	Psmb10	Proteasome subunit beta type-10	3	2	1	166.5	Novel	NAD_Gly3P_dh_C, Pr_beta_C, Proteasome
`ENSMUSG00000024180`	Pgap6	Post-GPI attachment to proteins factor 6	2	2	1	5.9	Ref	DUF3522, LtrA, SLATT_3
`ENSMUSG00000055762`	Eef1d	Elongation factor 1-delta	7	2	1	54.8	Mixed	CCD48, EF-1_beta_acid, EF1_GNE, JIP_LZII, KASH_CCD, Lzipper-MIP1, PRKG1_interact, PilJ, RF-1, Striatin, TSC22, TolA_bind_tri, Trimer_CC
`ENSMUSG00000040276`	Pacsin1	Protein kinase C and casein kinase substrate in neurons protein 1	4	2	1	39.2	Ref	DUF2605, FCH, RPS31, SH3_1, SH3_2, SH3_9, Terpene_syn_C_2
`ENSMUSG00000007950`	Abhd8	Protein ABHD8	2	2	1	266.4	Novel	Abhydrolase_1, Abhydrolase_2, Abhydrolase_4, Abhydrolase_6, Abhydrolase_8, BD-FAE, Chlorophyllase2, DLH, DUF2920, DUF4663, FSH1, HofP, Hydrolase_4, LIP, Peptidase_S15, Peptidase_S9, Ser_hydrolase, Trypan_PARP
`ENSMUSG00000033916`	Chmp2a	Charged multivesicular body protein 2a	3	2	1	477.9	Ref	FapA, Snf7, YlqD
`ENSMUSG00000060703`	Cd302	CD302 antigen	2	2	1	166.5	Ref	DUF973, IN_DBD_C, Lectin_C, UL45
`ENSMUSG00000002625`	Akap8l	A-kinase anchor protein 8-like	2	2	1	31.3	Ref	AKAP95, CDC45, SURF2, zf-C2H2_2
`ENSMUSG00000032121`	Tmem218	Transmembrane protein 218	2	2	1	5.9	Novel	CbiQ, DUF4395, DUF6418
`ENSMUSG00000031485`	Plpbp	Pyridoxal phosphate homeostasis protein	2	2	1	37.2	Ref	Ala_racemase_N, Homez
`ENSMUSG00000034430`	Zxdc	Zinc finger protein ZXDC	2	2	1	5.9	Ref	FOXP-CC, Ogr_Delta, Spt46, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_aberr, zf-H2C2_2, zf_ZIC
`ENSMUSG00000027942`	—	Protein C1orf43 homolog	3	2	1	11.8	Mixed	6PGD, NICE-3
`ENSMUSG00000089917`	Uckl1	Uridine-cytidine kinase-like 1	2	2	1	31.3	Ref	AAA_17, AAA_18, AAA_33, ABC_tran, CPT, CoaE, Cytidylate_kin, PRK, UPRTase, Zeta_toxin
`ENSMUSG00000025495`	Ptdss2	Phosphatidylserine synthase 2	2	2	1	7.8	Ref	DUF3382, Gemini_mov, HemY_N, PSS, TctB
`ENSMUSG00000020409`	Slu7	Pre-mRNA-splicing factor SLU7	2	2	1	7.8	Novel	Cadherin_tail, DNA_pol_D_N, Rtf2, Slu7, zf-CCHC, zf-CCHC_4
`ENSMUSG00000037089`	Slc35b2	Adenosine 3'-phospho 5'-phosphosulfate transporter 1	2	2	1	27.4	Ref	DUF2070, EamA, PI3K_rbd, TPT, UAA
`ENSMUSG00000028049`	Scamp3	Secretory carrier-associated membrane protein 3	2	2	1	115.6	Ref	HCV_NS5a_C, LicD, MIC19_MIC25, Phage_holin_3_6, SCAMP
`ENSMUSG00000032940`	Rbm11	Splicing regulator RBM11	2	2	1	0.0	Ref	Cas_Cas2CT1978, DUF5746, Med19, RRM_1, RRM_5
`ENSMUSG00000051504`	CD33	Myeloid cell surface antigen CD33	5	2	1	9.8	Ref	C2-set, C2-set_2, DUF2490, I-set, Ig_3, V-set, ig
`ENSMUSG00000060475`	Wtap	Pre-mRNA-splicing regulator WTAP	2	2	1	5.9	Ref	ADIP, ALMT, ATG14, Bcr-Abl_Oligo, CENP-H, DUF2000, DUF2120, DUF6468, DUF6594, SKA2, SPACA9, Wtap
`ENSMUSG00000003421`	Nosip	Nitric oxide synthase-interacting protein	2	2	1	68.6	Ref	PWI, Rtf2, U-box, zf-NOSIP, zf-Nse
`ENSMUSG00000056121`	Fez2	Fasciculation and elongation protein zeta-2	2	2	1	35.3	Ref	DUF5102, FEZ, FapA, Gluconate_2-dh3, MRP-L46, NPV_P10, V_ATPase_I
`ENSMUSG00000026207`	Speg	Striated muscle-specific serine/threonine-protein kinase	3	2	1	45.0	Mixed	C2-set_2, I-set, Ig_2, Ig_3, SPEG_u2, V-set, ig
`ENSMUSG00000004268`	Emg1	Ribosomal RNA small subunit methyltransferase NEP1	2	2	1	115.6	Novel	CHMI, EMG1
`ENSMUSG00000018405`	Mrm1	rRNA methyltransferase 1, mitochondrial	2	2	1	5.9	Novel	Methyltrn_RNA_4, SpoU_methylase, SpoU_sub_bind
`ENSMUSG00000044533`	Rps2	Small ribosomal subunit protein uS5	3	2	1	82.3	Novel	Ribosomal_S5, Ribosomal_S5_C
`ENSMUSG00000044221`	Grsf1	G-rich sequence factor 1	2	2	1	19.6	Novel	RRM_1, zf-C2H2_assoc
`ENSMUSG00000029291`	Rufy3	Protein RUFY3	2	2	1	13.7	Ref	DUF2344, DUF5595, DUF641, RUN, Ribosomal_S8, SlyX, Trimer_CC
`ENSMUSG00000035984`	Nme5	Nucleoside diphosphate kinase homolog 5	2	2	1	37.2	Ref	Dpy-30, NDK
`ENSMUSG00000019302`	Atp6v0a1	V-type proton ATPase 116 kDa subunit a 1	3	2	1	56.8	Ref	V_ATPase_I
`ENSMUSG00000028524`	Sgip1	SH3-containing GRB2-like protein 3-interacting protein 1	2	2	1	37.2	Ref	ATG29_N, Adap_comp_sub, TGBp3, muHD
`ENSMUSG00000052926`	Rnaseh2a	Ribonuclease H2 subunit A	3	2	1	39.2	Ref	ALG11_N, RNase_HII
`ENSMUSG00000020608`	Smc6	Structural maintenance of chromosomes protein 6	2	2	1	7.8	Ref	AAA_15, AAA_18, AAA_21, AAA_23, AAA_27, AAA_29, ABC_tran, DUF3993, DUF5798, Exonuc_VII_L, FtsK_SpoIIIE, SMC_N, TPR_MLP1_2
`ENSMUSG00000021486`	RAB24	Ras-related protein Rab-24	2	2	1	711.0	Ref	AAA_24, PRELI, Ras, Roc
`ENSMUSG00000030435`	U2AF2	Splicing factor U2AF 65 kDa subunit	3	2	1	15.7	Novel	AIRS, CDC45, RRM_1, RRM_7, RRM_occluded, Transformer
`ENSMUSG00000028949`	SMARCD3	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3	2	2	1	9.8	Ref	DUF5529, SWIB
`ENSMUSG00000045128`	Rpl18a	Large ribosomal subunit protein eL20	5	2	1	43.1	Ref	Ribosomal_L18A
`ENSMUSG00000035297`	Cops4	COP9 signalosome complex subunit 4	2	2	1	201.7	Novel	CSN4_RPN5_eIF3a, DinB, PCI, RPN7, TPR_12, TPR_14, TPR_7, TPR_8, YabA
`ENSMUSG00000049721`	Gal3st1	Galactosylceramide sulfotransferase	2	2	1	11.8	Ref	Gal-3-0_sulfotr, Sulfotransfer_3, TCL1_MTCP1
`ENSMUSG00000029408`	Abcb9	ABC-type oligopeptide transporter ABCB9	2	2	1	0.0	Novel	AAA, AAA_10, AAA_15, AAA_16, AAA_21, AAA_22, AAA_23, AAA_29, AAA_30, ABC_ATPase, ABC_membrane, ABC_membrane_3, ABC_tran, ATP_bind_1, DUF4191, MCU, RsgA_GTPase, SMC_N, SbcC_Walker_B
`ENSMUSG00000002205`	Vrk3	Serine/threonine-protein kinase VRK3	2	2	1	41.1	Novel	Cys_rich_CPXG, DNA_ligase_ZBD, DUF2116, DZR, DZR_2, HMBD, HypA, Nudix_N_2, PK_Tyr_Ser-Thr, Pkinase, RNA_POL_M_15KD, UPF0547, YacG, zf-C2H2, zf-Di19, zf-NADH-PPase, zf-ribbon_3, zf_UBZ, zinc-ribbons_6, zinc_ribbon_15, zinc_ribbon_2
`ENSMUSG00000024826`	Dpf2	Zinc finger protein ubi-d4	2	2	1	31.3	Ref	C1_1, DPF1-3_N, Lar_restr_allev, PHD, PHD_2, PHD_4, zf-C2H2, zf-C2H2_4, zf-H2C2_2, zf-PHD-like
`ENSMUSG00000038695`	Josd2	Josephin-2	6	2	1	129.3	Mixed	Josephin
`ENSMUSG00000041268`	Dmxl2	DmX-like protein 2	2	2	1	50.9	Ref	ANAPC4_WD40, DJ-1_PfpI, Opiods_neuropep, Rav1p_C, WD40, eIF2A
`ENSMUSG00000036352`	Ubac1	Ubiquitin-associated domain-containing protein 1	2	2	1	60.7	Ref	APG6_N, CENP-H, DUF4878, HEAT_2, Rad60-SLD_2, RecX, STI1, TCP, UBA, UBA_2, UBA_3, WIYLD, ubiquitin
`ENSMUSG00000024308`	Tapbp	Tapasin	2	2	1	9.8	Ref	C1-set, Ig_3, Med5, ig
`ENSMUSG00000036381`	P2ry14	P2Y purinoceptor 14	2	2	1	2.0	Ref	7tm_1, Bac_export_2, DUF4834, Ni_hydr_CYTB, PDR_CDR, TMIE
`ENSMUSG00000027829`	Ccnl1	Cyclin-L1	2	2	1	9.8	Ref	Cyclin_C, Cyclin_C_2, Cyclin_N, K_channel_TID, Robl_LC7, TFIIB
`ENSMUSG00000021661`	Ankra2	Ankyrin repeat family A protein 2	2	2	1	11.8	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, Fer4_14, GIP1_N
`ENSMUSG00000006288`	Ttc5	Tetratricopeptide repeat protein 5	2	2	1	66.6	Ref	ANAPC3, Abi_C, CK2S, DUF6584, MAS20, RPN7, TPR-S, TPR_1, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_8, TPR_9, TTC5_OB
`ENSMUSG00000028519`	Dab1	Disabled homolog 1	3	2	1	23.5	Novel	Bac_globin, PID
`ENSMUSG00000034361`	Cpne2	Copine-2	2	2	1	13.7	Ref	C2, Copine, VMAP-M1, vWA-TerF-like
`ENSMUSG00000032046`	Abhd12	Lysophosphatidylserine lipase ABHD12	2	2	1	164.5	Novel	Abhydrolase_1, Abhydrolase_11, Abhydrolase_2, Abhydrolase_3, Abhydrolase_6, BD-FAE, DUF900, Hydrolase_4, LIP, Peptidase_S15, Peptidase_S9, Say1_Mug180, TRP_2, Thioesterase
`ENSMUSG00000029471`	Camkk2	Calcium/calmodulin-dependent protein kinase kinase 2	2	2	1	29.4	Ref	ABC1, APH, DUF4010, HAND, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Seadorna_VP7
`ENSMUSG00000002289`	Angptl4	Angiopoietin-related protein 4	2	2	1	5.9	Ref	COG5, DUF16, DUF5798, DivIC, Fib_alpha, Fibrinogen_C, Helo_like_N, HrpB7, HrpJ, SlyX, UPF0242, XhlA
`ENSMUSG00000003099`	Ppp5c	Serine/threonine-protein phosphatase 5	2	2	1	199.8	Novel	AAA_assoc_2, ANAPC3, MIT, Metallophos, PPP5, TPR_1, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000018669`	Cdk5rap3	CDK5 regulatory subunit-associated protein 3	2	2	1	11.8	Novel	CDK5RAP3, DUF3502, MbeD_MobD
`ENSMUSG00000027937`	Jtb	Protein JTB	2	2	1	135.1	Novel	DUF1505, DUF3703, JTB
`ENSMUSG00000029780`	Nt5c3a	Cytosolic 5'-nucleotidase 3A	2	2	1	70.5	Ref	ACT_7, CIDE-N, DZR_2, HAD, Hydrolase, UMPH-1, zf-ANAPC11
`ENSMUSG00000025041`	Nt5c2	Cytosolic purine 5'-nucleotidase	2	2	1	3.9	Ref	5_nucleotid, BCAS2, DUF2095, Filament, Filament_head, bZIP_2
`ENSMUSG00000044811`	Cd300c2	CMRF-35-like molecule 4	2	2	1	35.3	Ref	Apq12, Ig_3, V-set, ig
`ENSMUSG00000033998`	Kcnk1	Potassium channel subfamily K member 1	3	2	1	146.9	Novel	DUF6097, Ion_trans, Ion_trans_2
`ENSMUSG00000030032`	Wdr54	WD repeat-containing protein 54	2	2	1	21.5	Ref	ANAPC4_WD40, Anillin, Lgl_C, PQQ, RAB3GAP2_N, WD40
`ENSMUSG00000078580`	ZNF764	Zinc finger protein 764	2	2	1	3.9	Ref	DUF6076, DUF899, FYDLN_acid, HVO_2753_ZBP, HypA, KRAB, PhnJ, Zn-ribbon_8, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_9, zf-C2H2_jaz, zf-Di19, zf-H2C2_2, zf-LYAR, zf-met, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000021134`	Srsf5	Serine/arginine-rich splicing factor 5	4	2	1	146.9	Ref	DbpA, Nup35_RRM_2, RRM_1, RRM_3, RRM_5, RRM_occluded
`ENSMUSG00000041353`	FAM156A	Protein FAM156A/FAM156B	3	2	1	13.7	Ref	PGC7_Stella
`ENSMUSG00000024064`	Galnt14	Polypeptide N-acetylgalactosaminyltransferase 14	2	2	1	19.6	Ref	DUF4519, Glyco_tranf_2_2, Glyco_transf_7C, Glycos_transf_2, Ricin_B_lectin
`ENSMUSG00000056116`	H2-Q10	H-2 class I histocompatibility antigen, Q10 alpha chain	4	2	1	27.4	Mixed	AA_permease_2, C1-set, C2-set_2, Cadherin_C_2, Ig_3, MHC_I, Mucin, ig
`ENSMUSG00000026279`	Thap4	Peroxynitrite isomerase THAP4	2	2	1	13.7	Ref	Csm1_N, DUF4201, HrpB7, Med21, THAP, THAP4_heme-bd, TMF_DNA_bd, Tropomyosin_1, YscO
`ENSMUSG00000061613`	U2AF1	Splicing factor U2AF 35 kDa subunit	3	2	1	225.2	Ref	KMP11, Nup35_RRM_2, RRM_1, Torus, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf_CCCH_4
`ENSMUSG00000032842`	Abcc10	ATP-binding cassette sub-family C member 10	2	2	1	5.9	Ref	AAA_16, AAA_21, AAA_22, AAA_23, AAA_29, AAA_30, ABC_membrane, ABC_tran, DO-GTPase2, DUF87, Dynamin_N, KAP_NTPase, MMR_HSR1, NB-ARC, RsgA_GTPase, SMC_N, Zeta_toxin
`ENSMUSG00000025920`	Stau2	Double-stranded RNA-binding protein Staufen homolog 2	2	2	1	31.3	Ref	DND1_DSRM, LPP20, Staufen_C, dsrm
`ENSMUSG00000018189`	Uchl5	Ubiquitin carboxyl-terminal hydrolase isozyme L5	2	2	1	33.3	Novel	DUF2785, PNPase, Peptidase_C12, Rep_fac_C, S-methyl_trans, UCH_C
`ENSMUSG00000034867`	Ankrd27	Ankyrin repeat domain-containing protein 27	2	2	1	2.0	Novel	EcoEI_R_C, IDO, Ribonuc_red_sm, Sec8_exocyst, VPS9
`ENSMUSG00000005069`	Pex5	Peroxisomal targeting signal 1 receptor	2	2	1	9.8	Ref	AAA_lid_7, ABATE, ANAPC3, DUF1641, Nsp1_C, PPTA, SHNi-TPR, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_20, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000009207`	Lnpk	Endoplasmic reticulum junction formation protein lunapark	2	2	1	7.8	Ref	DUF2207, Osmo_CC, zinc_ribbon_10
`ENSMUSG00000003872`	Lin7b	Protein lin-7 homolog B	3	2	1	239.0	Novel	L27, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000026179`	Pnkd	Probable thioesterase PNKD	3	2	1	172.4	Ref	HAGH_C, Lactamase_B, Lactamase_B_2, Lactamase_B_3
`ENSMUSG00000024792`	Zfpl1	Zinc finger protein-like 1	2	2	1	64.6	Ref	DUF35_N, PHD, PHD_4, zf-4CXXC_R1, zf-B_box, zf-RING_16, zf-RING_2
`ENSMUSG00000030704`	Rab6a	Ras-related protein Rab-6A	2	2	1	27.4	Ref	Arf, CPT, FeoB_N, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB, TetR_C_16
`ENSMUSG00000071645`	Tut1	Speckle targeted PIP5K1A-regulated poly(A) polymerase	2	2	1	15.7	Ref	PAP_assoc, RL, RRM_1, RRM_7, TUTase, zf-C2H2_2, zf-C2H2_jaz, zf-met
`ENSMUSG00000017421`	Znf207	BUB3-interacting and GLEBS motif-containing protein ZNF207	2	2	1	13.7	Ref	SSP160, zf-C2H2, zf-C2H2_4, zf-FCS
`ENSMUSG00000026696`	VAMP4	Vesicle-associated membrane protein 4	3	2	1	41.1	Ref	DUF1664, Fzo_mitofusin, MCU, NPV_P10, Nuf2_DHR10-like, Synaptobrevin, TFR_dimer
`ENSMUSG00000040667`	Nup88	Nuclear pore complex protein Nup88	2	2	1	17.6	Ref	BLOC1_2, DUF4208, DUF4404, Exonuc_VII_L, FliJ, Importin_rep_6, Latarcin, Nup88
`ENSMUSG00000013593`	Ndufs2	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial	2	2	1	284.0	Novel	Complex1_49kDa, NiFeSe_Hases
`ENSMUSG00000022789`	Dnm1l	Dynamin-1-like protein	2	2	1	23.5	Ref	AAA_16, AAA_28, Dynamin_M, Dynamin_N, FtsK_SpoIIIE, GED, MCM6_C, MMR_HSR1, Pro_3_hydrox_C, TIMELESS
`ENSMUSG00000032504`	Pdcd6ip	Programmed cell death 6-interacting protein	2	2	1	27.4	Ref	AAT, ALIX_LYPXL_bnd, BRO1, DUF5082, Ly49, Tex_N
`ENSMUSG00000006095`	Tbcb	Tubulin-folding cofactor B	2	2	1	376.1	Ref	CAP_GLY, RSV_NS2, Ubiquitin_2
`ENSMUSG00000024026`	Glo1	Lactoylglutathione lyase	3	2	1	117.5	Ref	Glyoxalase, Glyoxalase_4, Glyoxalase_6, PIG-X
`ENSMUSG00000031167`	Rbm3	RNA-binding protein 3	5	2	1	397.6	Ref	RRM_1, RRM_7, RRM_occluded
`ENSMUSG00000026632`	Tatdn3	Putative deoxyribonuclease TATDN3	3	2	1	17.6	Ref	TatD_DNase
`ENSMUSG00000037257`	Aagab	Alpha- and gamma-adaptin-binding protein p34	2	2	1	2.0	Novel	Adaptin_binding, FMN_bind_2
`ENSMUSG00000074182`	Znhit6	Box C/D snoRNA protein 1	2	2	1	5.9	Ref	PolC_DP2, zf-B_box, zf-HIT
`ENSMUSG00000026270`	Capn10	Calpain-10	2	2	1	19.6	Ref	Calpain_III, Peptidase_C2
`ENSMUSG00000020932`	Gfap	Glial fibrillary acidic protein	2	2	1	70.5	Ref	Apolipoprotein, BLOC1_2, CENP-F_leu_zip, DUF1664, DUF4407, Fib_alpha, Filament, Filament_head, GAS, HU-CCDC81_bac_2, Rsa3, Snu56_snRNP, TPR_MLP1_2
`ENSMUSG00000027810`	Eif2a	Eukaryotic translation initiation factor 2A	3	2	1	25.5	Novel	SR-25, eIF2A
`ENSMUSG00000034403`	Pja1	E3 ubiquitin-protein ligase Praja-1	2	2	1	54.8	Ref	Amidase, FANCL_C, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000028991`	Mtor	Serine/threonine-protein kinase mTOR	2	2	1	56.8	Ref	ANAPC3, Adaptin_N, DUF3385, EPSP_synthase, FAT, FATC, FRB_dom, HEAT, HEAT_2, HEAT_EZ, Nipped-B_C, PI3_PI4_kinase, TAN, TPR_19, UME
`ENSMUSG00000028677`	Rnf220	E3 ubiquitin-protein ligase Rnf220	2	2	1	9.8	Ref	DZR, DZR_2, Endonuclease_7, Pox_A_type_inc, Prok-RING_4, RNF220, Rad50_zn_hook, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Nse, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-ribbon_3
`ENSMUSG00000035228`	Ccdc106	Coiled-coil domain-containing protein 106	4	2	1	31.3	Novel	CCDC106, G6B, SOGA
`ENSMUSG00000019471`	Cdc37	Hsp90 co-chaperone Cdc37	2	2	1	58.8	Novel	APG6_N, ATG16, Amidohydro_1, CATRA-C, CDC37_C, CDC37_M, CDC37_N, DUF1402, KfrA_N, LMBR1, Med4
`ENSMUSG00000057554`	Lgals8	Galectin-8	2	2	1	3.9	Ref	Gal-bind_lectin, WIF
`ENSMUSG00000044442`	Hemk2	Methyltransferase HEMK2	2	2	1	11.8	Ref	DUF5429, DUF938, MTS, Methyltransf_11, Methyltransf_12, Methyltransf_16, Methyltransf_24, Methyltransf_25, Methyltransf_31, Methyltransf_33, PCMT, PrmA, UPF0020
`ENSMUSG00000117789`	Brms1	Breast cancer metastasis-suppressor 1 homolog	2	2	1	2.0	Novel	CDC45, DASH_Spc19, Dynactin_p62, EBP50_C, Glyco_transf_49, GspL_C, PIP5K, Sds3, Vfa1, Zip
`ENSMUSG00000025781`	Atp5f1c	ATP synthase F(1) complex subunit gamma, mitochondrial	3	2	1	1,747.1	Ref	ACT_4, ATP-synt
`ENSMUSG00000032177`	Pde4a	3',5'-cyclic-AMP phosphodiesterase 4A	2	2	1	37.2	Ref	Emfourin, Lig_C, PDE4_UCR, PDEase_I
`ENSMUSG00000034321`	Exosc1	Exosome complex component CSL4	2	2	1	23.5	Novel	ECR1_N, EXOSC1
`ENSMUSG00000022234`	Cct5	T-complex protein 1 subunit epsilon	2	2	1	45.0	Novel	Cpn60_TCP1, aRib
`ENSMUSG00000013698`	Pea15	Astrocytic phosphoprotein PEA-15	3	2	1	133.2	Mixed	DED
`ENSMUSG00000058297`	Spock2	Testican-2	2	2	1	336.9	Novel	EF-hand_6, EF-hand_7, Kazal_1, Kazal_2, SPARC_Ca_bdg, Thyroglobulin_1
`ENSMUSG00000006392`	Med8	Mediator of RNA polymerase II transcription subunit 8	2	2	1	90.1	Ref	Baculo_PEP_C, CENP-Q, CSN4_RPN5_eIF3a, Med8, SlyX
`ENSMUSG00000031628`	Casp3	Caspase-3	2	2	1	3.9	Ref	Peptidase_C14, SICA_beta
`ENSMUSG00000037279`	Ovol2	Transcription factor Ovo-like 2	2	2	1	2.0	Novel	A2L_zn_ribbon, ADK_lid, C1_1, C5HCH, FYVE, FpoO, HEPN_RES_NTD1, Rubredoxin_2, XPA_N, YjdM_Zn_Ribbon, Zn-ribbon_8, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_3rep, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-H2C2_5, zf-MYST, zf-met, zf_C2H2_ZHX, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000017664`	Slc35c2	Solute carrier family 35 member C2	5	2	1	37.2	Mixed	EamA, SPC25, TPT, UAA
`ENSMUSG00000021619`	Atg10	Ubiquitin-like-conjugating enzyme ATG10	2	2	1	23.5	Ref	Autophagy_act_C, zinc_ribbon_4
`ENSMUSG00000050043`	Tmx2	Thioredoxin-related transmembrane protein 2	2	2	1	172.4	Ref	PAP2_3, Thioredoxin
`ENSMUSG00000021007`	Spata7	Spermatogenesis-associated protein 7 homolog	2	2	1	15.7	Ref	APG5, HSD3
`ENSMUSG00000033735`	Spr	Sepiapterin reductase	2	2	1	146.9	Ref	KR, Nodulin-like, adh_short, adh_short_C2
`ENSMUSG00000063236`	—	UPF0488 protein C8orf33 homolog	2	2	1	60.7	Ref	CtsR_C, DUF3584, DUF4615, PDE4_UCR, PolC_DP2
`ENSMUSG00000028863`	Meaf6	Chromatin modification-related protein MEAF6	3	2	1	17.6	Mixed	BBS2_C, NuA4
`ENSMUSG00000047261`	Gap43	Neuromodulin	2	2	1	513.2	Novel	HEPN_AbiU2, IQ, Neuromodulin, Neuromodulin_N
`ENSMUSG00000021087`	rtn1-a	Reticulon-1-A	3	2	1	1,770.7	Ref	DUF4736, Reticulon
`ENSMUSG00000038965`	UBE2L3	Ubiquitin-conjugating enzyme E2 L3	2	2	1	3.9	Ref	DUF1154, Prok-E2_B, RWD, UEV, UQ_con
`ENSMUSG00000026254`	Eif4e2	Eukaryotic translation initiation factor 4E type 2	5	2	1	15.7	Ref	FlgD, IF4E, IHABP4_N, Stm1_N
`ENSMUSG00000058267`	Mrps14	Small ribosomal subunit protein uS14m	2	2	1	180.2	Ref	PSS, Ribosomal_S14
`ENSMUSG00000031068`	Glrx3	Glutaredoxin-3	2	2	1	37.2	Novel	Arf, CNOT1_HEAT_N, DIM1, DUF4279, DUF953, GREB1_C, GST_N_3, GerD, Glutaredoxin, HeH, HyaE, OST3_OST6, Phosducin, RdRP, SH3BGR, TMF_TATA_bd, Thioredoxin, Thioredoxin_2, Thioredoxin_8, Thioredoxin_9, TrbC_Ftype, ZinT
`ENSMUSG00000008167`	FBXW9	F-box/WD repeat-containing protein 9	2	2	1	13.7	Ref	F-box, F-box-like, PQQ_3, WD40, WD40_2, WD40_like
`ENSMUSG00000015714`	Cers2	Ceramide synthase 2	2	2	1	49.0	Novel	Homeodomain, Homez, TRAM1, TRAM_LAG1_CLN8, UPF0767
`ENSMUSG00000030795`	Fus	RNA-binding protein FUS	3	2	1	19.6	Ref	RRM_1, zf-RanBP
`ENSMUSG00000033595`	Lgi3	Leucine-rich repeat LGI family member 3	2	2	1	76.4	Ref	EPTP, LRRCT, LRR_1, LRR_4, LRR_5, LRR_8, LRR_9, RCDG1, stn_TNFRSF12A
`ENSMUSG00000070564`	Ntn5	Netrin-5	2	2	1	11.8	Ref	Laminin_EGF, Laminin_N, NTR
`ENSMUSG00000030846`	TIAL1	Nucleolysin TIAR	3	2	1	21.5	Ref	Calcipressin, DbpA, Nup35_RRM, Nup35_RRM_2, RRM_1, RRM_3, RRM_7, RRM_occluded, XOO_2897-deam
`ENSMUSG00000037706`	Cd81	CD81 antigen	2	2	1	1,269.2	Novel	DUF6768, Tetraspanin
`ENSMUSG00000026027`	Stradb	STE20-related kinase adapter protein beta	2	2	1	13.7	Ref	DUF1824, ISET-FN3_linker, Kinase-like, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000027624`	Epb41l1	Band 4.1-like protein 1	2	2	1	15.7	Ref	4_1_CTD, DUF6426, FA, FERM_C, FERM_M, FERM_N, SAB
`ENSMUSG00000028936`	RPL22	Large ribosomal subunit protein eL22	5	2	1	176.3	Mixed	Ribosomal_L22e, SAM_KSR1
`ENSMUSG00000028180`	ZRANB2	Zinc finger Ran-binding domain-containing protein 2	2	2	1	13.7	Ref	DUF35_N, DZR, zf-RanBP
`ENSMUSG00000036371`	Serbp1	SERPINE1 mRNA-binding protein 1	4	2	1	146.9	Ref	HABP4_PAI-RBP1, IHABP4_N
`ENSMUSG00000107068`	Pagr1a	PAXIP1-associated glutamate-rich protein 1A	2	2	1	90.1	Novel	CD225, Nop53, PAXIP1_C
`ENSMUSG00000026020`	Nop58	Nucleolar protein 58	2	2	1	13.7	Ref	Cellulose_synt, DUF2247, MC6, NOP5NT, Nop
`ENSMUSG00000021660`	Btf3	Transcription factor BTF3	2	2	1	703.2	Ref	LolA, Myco_19_kDa, NAC
`ENSMUSG00000028789`	Azin2	Antizyme inhibitor 2	2	2	1	3.9	Ref	AP_endonuc_2, FeoC, Orn_Arg_deC_N, Orn_DAP_Arg_deC
`ENSMUSG00000015094`	Npdc1	Neural proliferation differentiation and control protein 1	2	2	1	1,059.6	Ref	LapA_dom, NPDC1
`ENSMUSG00000022961`	Son	Protein SON	2	2	1	52.9	Ref	DND1_DSRM, G-patch, G-patch_2, RSRP, dsrm
`ENSMUSG00000038000`	Acd	Adrenocortical dysplasia protein	3	2	1	19.6	Novel	OGFr_III, TEBP_beta, TPP1
`ENSMUSG00000060036`	Rpl3	Large ribosomal subunit protein uL3	2	2	1	2,595.3	Ref	DUF6709, Ribosomal_L3
`ENSMUSG00000032356`	Rasgrf1	Ras-specific guanine nucleotide-releasing factor 1	3	2	1	174.3	Ref	PH, PH_11, PH_20
`ENSMUSG00000005312`	Ubqln1	Ubiquilin-1	2	2	1	19.6	Ref	CUE, ComX, DnaG_DnaB_bind, Dsc3_N, HOIP-UBA, PDH_C, Rad60-SLD, Rad60-SLD_2, STI1, UBA, Ubiquitin_4, Ubiquitin_5, XPC-binding, YukD, ubiquitin
`ENSMUSG00000025420`	Katnal2	Katanin p60 ATPase-containing subunit A-like 2	2	2	1	3.9	Ref	AAA, AAA_14, AAA_16, AAA_18, AAA_22, AAA_24, AAA_25, AAA_28, AAA_33, AAA_5, AAA_7, AAA_lid_3, DUF815, IstB_IS21, LisH, Mg_chelatase, NACHT, NB-ARC, Parvo_NS1, PhoH, RNA_helicase, RuvB_N, TIP49, TsaE, bpMoxR
`ENSMUSG00000055026`	Gabrg3	Gamma-aminobutyric acid receptor subunit gamma-3	2	2	1	13.7	Ref	Neur_chan_LBD, Neur_chan_memb, YesK
`ENSMUSG00000074874`	Ctla2a	Protein CTLA-2-alpha	2	2	1	7.8	Ref	GST_N_5, Inhibitor_I29, Mem_trans
`ENSMUSG00000029440`	Psmd9	26S proteasome non-ATPase regulatory subunit 9	3	2	1	23.5	Mixed	GRASP55_65, Nas2_N, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000028759`	Hp1bp3	Heterochromatin protein 1-binding protein 3	2	2	1	11.8	Ref	CCD97-like_C, EMC7_beta-sandw, Linker_histone, Nop14, RRN3, SDA1, SURF2, YL1
`ENSMUSG00000006736`	Tspan31	Tetraspanin-31	2	2	1	139.1	Novel	Ac76, DUF6524, Herpes_LMP1, PHO4, Tetraspanin, YrzO
`ENSMUSG00000006575`	Rundc3a	RUN domain-containing protein 3A	3	2	1	111.6	Ref	DUF3450, DUF5595, Phage_GP20, RUN, UPF0242, WT1
`ENSMUSG00000002343`	Armc6	Armadillo repeat-containing protein 6	2	2	1	52.9	Novel	Arm, Atx10homo_assoc, CRS1_YhbY, ELMO_ARM, Mis14, UBA_3
`ENSMUSG00000040888`	Dst	Dystonin	2	2	1	13.7	Ref	APOBEC4, DUF5344, DUF6730, Evr1_Alr, FAD_SOX, GrpE, NCKAP5, Nbl1_Borealin_N, SNAD4, Spectrin
`ENSMUSG00000036564`	ndrg4	Protein NDRG4	3	2	1	152.8	Ref	Abhydrolase_1, Endotoxin_N, Ndr
`ENSMUSG00000033434`	Gtpbp6	Putative GTP-binding protein 6	4	2	1	17.6	Novel	AAA_16, AAA_23, ABC_tran, DO-GTPase2, Dynamin_N, FeoB_N, GTP-bdg_M, GTP-bdg_N, HflX_C, MMR_HSR1, MobB, PduV-EutP, Rad17, TniB, cobW
`ENSMUSG00000029755`	Dlx5	Homeobox protein DLX-5	2	2	1	23.5	Ref	Cauli_DNA-bind, DLL_N, Homeobox_KN, Homeodomain, YdaS_antitoxin
`ENSMUSG00000022856`	Tmem41a	Transmembrane protein 41A	2	2	1	35.3	Ref	SNARE_assoc, ThiW
`ENSMUSG00000025646`	Atrip	ATR-interacting protein	2	2	1	15.7	Ref	FapA, Fez1, Golgin_A5, HIP1_clath_bdg, HMMR_N, Lebercilin, Med21, OmpH, RNase_H_2, RNase_T, Tropomyosin_1
`ENSMUSG00000004207`	Psap	Prosaposin	3	2	1	521.0	Ref	CYSTM, DUF3456, DUF6487, SapA, SapB_1, SapB_2
`ENSMUSG00000026344`	Lypd1	Ly6/PLAUR domain-containing protein 1	2	2	1	31.3	Ref	7tm_1, ATP-synt_I, Peptidase_M50B, UPAR_LY6_2
`ENSMUSG00000022336`	EIF3E	Eukaryotic translation initiation factor 3 subunit E	2	2	1	229.2	Novel	CSN8_PSD8_EIF3K, HD_assoc, PARP_reg, PCI, SPESP1, VMAP-M1, eIF3_N
`ENSMUSG00000030621`	Me3	NADP-dependent malic enzyme, mitochondrial	4	2	1	3.9	Novel	Malic_M, malic
`ENSMUSG00000053799`	Exoc6	Exocyst complex component 6	2	2	1	5.9	Ref	AKAP7_NLS, COG5, DUF5856, Sec15
`ENSMUSG00000034868`	MYL12B	Myosin regulatory light chain 12B	2	2	1	350.6	Novel	2-Hacid_dh_C, DDE_Tnp_IS66_C, DUF2267, DUF2666, DUF3741, EF-hand_1, EF-hand_11, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, GPHH, SPARC_Ca_bdg, SurA_N_2, UPF0154
`ENSMUSG00000031843`	Mphosph6	M-phase phosphoprotein 6	2	2	1	64.6	Ref	MPP6, TilS
`ENSMUSG00000026858`	Miga2	Mitoguardin 2	2	2	1	5.9	Ref	DUF3824, Miga
`ENSMUSG00000031922`	Cep57	Centrosomal protein of 57 kDa	2	2	1	7.8	Ref	ADIP, Cep57_CLD, Cep57_MT_bd, Crescentin, DUF1664, Occludin_ELL, TMF_TATA_bd, TPR_MLP1_2, Tankyrase_bdg_C
`ENSMUSG00000037364`	Srrt	Serrate RNA effector molecule homolog	2	2	1	11.8	Ref	ARS2, DUF4187, DUF5923, FAM184, Hid1, RRM_1, RR_TM4-6, SAPS, SERRATE_Ars2_N, SLC12, Zip
`ENSMUSG00000025170`	Rab40b	Ras-related protein Rab-40B	3	2	1	43.1	Novel	Arf, Ras, Roc, SOCS_box
`ENSMUSG00000090015`	Znf431	Zinc finger protein 431	2	2	1	2.0	Ref	BDHCT_assoc, C1_1, C1_4, KRAB, XPA_N, ZZ, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-Paramyx-P, zf-trcl, zf_UBZ, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000024436`	Mrps18b	Small ribosomal subunit protein mS40	2	2	1	129.3	Ref	FAM165, HTH_21, Ribosomal_S18, zf-RING_10
`ENSMUSG00000050088`	—	UPF0669 protein C6orf120 homolog	3	2	1	7.8	Ref	Colicin_immun, Tox-MPTase2, UPF0669
`ENSMUSG00000056055`	Sag	S-arrestin	2	2	1	2.0	Novel	Arrestin_C, Arrestin_N, Transglut_C
`ENSMUSG00000039128`	Cdc123	Translation initiation factor eIF2 assembly protein	3	2	1	45.0	Mixed	D123
`ENSMUSG00000018395`	Kif3a	Kinesin-like protein KIF3A	2	2	1	76.4	Novel	AAA_22, AAA_30, DUF1492, DUF4407, DUF5677, Kinesin, Kinesin_assoc, Microtub_bd, PIF1
`ENSMUSG00000026701`	Prdx6	Peroxiredoxin-6	4	2	1	407.4	Ref	1-cysPrx_C, AhpC-TSA, Redoxin
`ENSMUSG00000007097`	Atp1a2	Sodium/potassium-transporting ATPase subunit alpha-2	2	2	1	82.3	Novel	Cation_ATPase, Cation_ATPase_C, Cation_ATPase_N, E1-E2_ATPase, HAD, Hydrolase, Hydrolase_3, Oxidored_q3, Sigma70_r4
`ENSMUSG00000039431`	Mtmr7	Phosphatidylinositol-3-phosphate phosphatase MTMR7	2	2	1	29.4	Ref	DSPc, DUF5320, FapA, GRAM, Myotub-related, Vps36_ESCRT-II, Y_phosphatase, Y_phosphatase3
`ENSMUSG00000074247`	Dda1	DET1- and DDB1-associated protein 1	3	2	1	49.0	Ref	DDA1
`ENSMUSG00000030108`	Slc6a13	Sodium- and chloride-dependent GABA transporter 2	2	2	1	9.8	Ref	DUF2829, SNF
`ENSMUSG00000027166`	Dnajc24	DnaJ homolog subfamily C member 24	2	2	1	41.1	Ref	DnaJ, Peptidase_S77, zf-CSL
`ENSMUSG00000004187`	Kifc2	Kinesin-like protein KIFC2	2	2	1	49.0	Novel	Baculo_PEP_C, DUF4407, DUF6779, Fib_alpha, Kinesin, Microtub_bd, Rubis-subs-bind, TACC_C, THOC7
`ENSMUSG00000042165`	Adrm1	Proteasomal ubiquitin receptor ADRM1	2	2	1	105.8	Novel	ASXH, DUF1631, Proteasom_Rpn13, RPN13_C
`ENSMUSG00000025967`	Eef1b	Elongation factor 1-beta	2	2	1	105.8	Ref	EF-1_beta_acid, EF1_GNE, GST_C_3, GST_C_6
`ENSMUSG00000052337`	Immt	MICOS complex subunit Mic60	3	2	1	52.9	Ref	Mitofilin
`ENSMUSG00000060149`	Znf431	Zinc finger protein 431	2	2	1	3.9	Ref	ATR13, AraC_N, BTHB, Baculo_LEF-11, CHU_C, Crinkler, Csm1, DUF2172, DUF5770, DUF5785, DUF5791, DUF6434, DUF6455, DnaB, KRAB, LZ3wCH, Linker_histone, McyA_C, NCD1, RNA_POL_M_15KD, SSXT, Sld7_C, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2HE, zf-H2C2_2, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000025875`	Tspan17	Tetraspanin-17	3	2	1	41.1	Mixed	Tetraspanin
`ENSMUSG00000033701`	Acbd6	Acyl-CoA-binding domain-containing protein 6	2	2	1	37.2	Ref	ACBP, Ank, Ank_2, Ank_3, Ank_4, Ank_5
`ENSMUSG00000024926`	Kat5	Histone acetyltransferase KAT5	3	2	1	23.5	Ref	Acetyltransf_1, Acetyltransf_7, MOZ_SAS, Tudor-knot, zf-MYST
`ENSMUSG00000025534`	Gusb	Beta-glucuronidase	2	2	1	15.7	Ref	Glyco_hydro_2, Glyco_hydro_2_C, Glyco_hydro_2_N
`ENSMUSG00000030083`	Abtb1	Ankyrin repeat and BTB/POZ domain-containing protein 1	2	2	1	50.9	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, BACK, BTB
`ENSMUSG00000031153`	Gripap1	GRIP1-associated protein 1	2	2	1	21.5	Ref	Geminin, PI3K_P85_iSH2, Rootletin, UQCC3
`ENSMUSG00000038141`	TMEM181	Transmembrane protein 181	2	2	1	11.8	Ref	Abhydrolase_9_N, DUF6709, MIG-14_Wnt-bd, YoqO
`ENSMUSG00000026860`	Sh3glb2	Endophilin-B2	5	2	1	193.9	Mixed	BAR, BAR_2, BAR_3, IMD, SH3_1, SH3_2, SH3_9
`ENSMUSG00000030750`	Nsmce1	Non-structural maintenance of chromosomes element 1 homolog	2	2	1	92.1	Novel	C1_1, DZR_2, SMC_Nse1, TMEMspv1-c74-12, zf-C3HC4, zf-C3HC4_2, zf-RING-like
`ENSMUSG00000045969`	Ing1	Inhibitor of growth protein 1	2	2	1	13.7	Ref	CCDC106, CDC45, CIS_TMP, DDHD, DUF4661, DUF6739, Dsh_C, Hid1, ING, KH_5, MAS20, MCM_bind, NPR3, PHD, PHD_2, Presenilin, SRP-alpha_N, Snu56_snRNP, ZYG-11_interact, Zip, zf-C4pol, zf-HC5HC2H
`ENSMUSG00000014771`	Pdcd2	Programmed cell death protein 2	2	2	1	9.8	Novel	PDCD2_C, zf-CSL, zf-HIT, zf-MYND
`ENSMUSG00000032186`	Tmod2	Tropomodulin-2	3	2	1	25.5	Ref	LRR_6, Peptidase_M3_N, Tropomodulin
`ENSMUSG00000002504`	Nherf2	Na(+)/H(+) exchange regulatory cofactor NHE-RF2	3	2	1	15.7	Ref	DUF5994, EBP50_C, GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000069520`	Tmem19	Transmembrane protein 19	3	2	1	2.0	Ref	DUF92
`ENSMUSG00000015149`	Sirt2	NAD-dependent protein deacetylase sirtuin-2	3	2	1	286.0	Ref	RecQ_Zn_bind, SIR2, YdjO
`ENSMUSG00000016349`	Eef1a2	Elongation factor 1-alpha 2	3	2	1	29.4	Novel	Dynamin_N, G-alpha, GTP_EFTU, GTP_EFTU_D2, GTP_EFTU_D3, GTP_EFTU_D4, MMR_HSR1
`ENSMUSG00000043419`	Rnf227	RING finger protein 227	2	2	1	336.9	Ref	DUF4632, FANCL_C, Prok-RING_4, RHH_4, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000026095`	Asnsd1	Asparagine synthetase domain-containing protein 1	2	2	1	13.7	Ref	Asn_synthase, GATase_6, GATase_7, NAD_synthase, Prefoldin_2
`ENSMUSG00000059796`	EIF4A1	Eukaryotic initiation factor 4A-I	2	2	1	468.1	Ref	AAA_19, AAA_22, AAA_30, CDC37_C, DEAD, Flavi_DEAD, Helicase_C, Helicase_RecD, PhoH, ResIII, UvrD-helicase, tRNA-synt_1b
`ENSMUSG00000021713`	Ppwd1	Peptidylprolyl isomerase domain and WD repeat-containing protein 1	2	2	1	2.0	Ref	ANAPC4_WD40, DUF505, Ge1_WD40, NBCH_WD40, Pro_isomerase, WD40
`ENSMUSG00000033020`	Polr2f	DNA-directed RNA polymerases I, II, and III subunit RPABC2	2	2	1	137.1	Ref	BDHCT_assoc, RNA_pol_Rpb6
`ENSMUSG00000053768`	Chchd3	MICOS complex subunit Mic19	2	2	1	60.7	Novel	CHCH, DUF1690, DUF885, ECH_2, Fes1, MIC19_MIC25, NDUF_B7
`ENSMUSG00000058325`	Dock1	Dedicator of cytokinesis protein 1	2	2	1	3.9	Ref	DHR-2_Lobe_A, DHR-2_Lobe_B, DHR-2_Lobe_C, DOCK-C2, DOCK_N, DUF5643, SH3_1, SH3_2, SH3_9
`ENSMUSG00000028576`	Ift74	Intraflagellar transport protein 74 homolog	2	2	1	23.5	Novel	ATG17_like, Crescentin, DUF1192, Fez1, Spc7, Spectrin
`ENSMUSG00000006418`	Rnf114	E3 ubiquitin-protein ligase RNF114	2	2	1	68.6	Ref	DND1_DSRM, DUF5810, DZR_2, FOXP-CC, Methyltransf_15, Prok-RING_4, Rtf2, U-box, zf-BED, zf-C2H2, zf-C2H2_12, zf-C2H2_4, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Di19, zf-H2C2_2, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX, zf_C2HC_14
`ENSMUSG00000039697`	Ncoa7	Nuclear receptor coactivator 7	2	2	1	5.9	Ref	HAD_SAK_2, LysM, SOBP, SURF2, TLD
`ENSMUSG00000035949`	Fbxw2	F-box/WD repeat-containing protein 2	3	2	1	49.0	Ref	ANAPC4_WD40, DUF5570, F-box, F-box-like, NBCH_WD40, WD40
`ENSMUSG00000004069`	Dnaja3	DnaJ homolog subfamily A member 3, mitochondrial	2	2	1	25.5	Ref	Anti-TRAP, DnaJ, DnaJ_C, DnaJ_CXXCXGXG, Endonuclease_7, FtrD-like
`ENSMUSG00000021147`	Wdr37	WD repeat-containing protein 37	2	2	1	5.9	Ref	ANAPC4_WD40, CCDC90-like, DivIVA, FAM76, HALZ, IZUMO, NBCH_WD40, Nup160, WD40, YabA
`ENSMUSG00000108030`	RPL27A	Large ribosomal subunit protein uL15	2	2	1	3.9	Ref	Ribosomal_L27A, TraL
`ENSMUSG00000001999`	Blvra	Biliverdin reductase A	2	2	1	90.1	Novel	Biliv-reduc_cat, GFO_IDH_MocA, HYPK_UBA, NAD_binding_3, SAM_KSR1
`ENSMUSG00000032112`	Trappc4	Trafficking protein particle complex subunit 4	4	2	1	315.3	Mixed	PDZ, PDZ_6, Sedlin_N, Sybindin
`ENSMUSG00000036273`	Lrrk2	Leucine-rich repeat serine/threonine-protein kinase 2	2	2	1	5.9	Novel	AAA_16, AAA_22, AAA_23, AAA_29, AAA_33, AAA_7, ABC_tran, Ank_2, Ank_3, Ank_5, Arf, Arm, COR, FtsK_SpoIIIE, G-alpha, Gtr1_RagA, HEAT_EZ, Kdo, LRR_4, LRR_6, LRR_8, LRR_9, MMR_HSR1, MobB, NACHT, NB-ARC, PK_Tyr_Ser-Thr, PduV-EutP, Pkinase, Ras, ResIII, Roc, SRP54, T2SSE, TniB, TrwB_AAD_bind, cobW, dUTPase_2
`ENSMUSG00000022223`	Sdr39u1	Epimerase family protein SDR39U1	3	2	1	97.9	Novel	DUF1731
`ENSMUSG00000025591`	Tma16	Translation machinery-associated protein 16	2	2	1	7.8	Ref	DUF1910, Tma16
`ENSMUSG00000030638`	Sh3gl3	Endophilin-A3	2	2	1	15.7	Ref	BAR, BAR_2, BAR_3, PPP5, SH3_1, SH3_2, SH3_9
`ENSMUSG00000027286`	Lrrc57	Leucine-rich repeat-containing protein 57	4	2	1	50.9	Mixed	CSTF_C, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9
`ENSMUSG00000024037`	Wdr4	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4	2	2	1	7.8	Ref	Nup160, WD40
`ENSMUSG00000027900`	Dram2	DNA damage-regulated autophagy modulator protein 2	3	2	1	5.9	Ref	Bac_export_2, DUF2613, Frag1
`ENSMUSG00000079317`	TRAPPC2	Trafficking protein particle complex subunit 2	2	2	1	141.0	Ref	DUF4984, Sedlin_N, Sybindin
`ENSMUSG00000015217`	Hmgb3	High mobility group protein B3	4	2	1	31.3	Ref	CHDNT, CLEC16A_C, HMG_box, HMG_box_2, HMG_box_5, RNA_pol_3_Rpc31, Ribosomal_60s, YABBY
`ENSMUSG00000035443`	Thyn1	Thymocyte nuclear protein 1	5	2	1	80.3	Mixed	EVE
`ENSMUSG00000044066`	Cep68	Centrosomal protein of 68 kDa	2	2	1	17.6	Ref	Spectrin, zf-Mss51
`ENSMUSG00000018572`	Phf23	PHD finger protein 23	2	2	1	17.6	Ref	C1_1, EBV-NA3, Nha1_C, PHD, PHD_2, PHD_4, Pox_Ag35, RNA_pol_3_Rpc31, Ribosomal_60s, zf-HC5HC2H, zf-RING_9
`ENSMUSG00000027962`	Vcam1	Vascular cell adhesion protein 1	2	2	1	0.0	Ref	Adhes-Ig_like, C1-set, C2-set, C2-set_2, DUF2808, I-set, ICAM_N, Ig_2, Ig_3, Ig_4, Ig_5, Ig_6, Izumo-Ig, Receptor_2B4, V-set, ig
`ENSMUSG00000035521`	Gnptg	N-acetylglucosamine-1-phosphotransferase subunit gamma	3	2	1	129.3	Novel	AltA1, DPM3, PRKCSH, PRKCSH_1
`ENSMUSG00000029128`	Rab28	Ras-related protein Rab-28	2	2	1	56.8	Ref	Arf, DLIC, G-alpha, Gtr1_RagA, PduV-EutP, Ras, Roc, WHAMM-JMY_N, XFP_N
`ENSMUSG00000024539`	Ptpn2	Tyrosine-protein phosphatase non-receptor type 2	2	2	1	25.5	Ref	CHASE3, DSPc, RIC3, Y_phosphatase, Y_phosphatase3
`ENSMUSG00000056770`	Setd3	Actin-histidine N-methyltransferase	2	2	1	60.7	Novel	Pertussis_S2S3, Rubis-subs-bind, SET
`ENSMUSG00000050471`	Fam118b	Protein FAM118B	2	2	1	7.8	Ref	DUF5443, SIR2_2
`ENSMUSG00000019970`	Sgk1	Serine/threonine-protein kinase Sgk1	3	2	1	74.4	Ref	ABC1, APH, FTA2, Kdo, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_C
`ENSMUSG00000033854`	Kcnk10	Potassium channel subfamily K member 10	2	2	1	3.9	Ref	DUF2390, DUF6594, ECF-ribofla_trS, Ion_trans, Ion_trans_2, PDZ_3, Xpo1
`ENSMUSG00000024939`	Fam89b	Leucine repeat adapter protein 25	2	2	1	31.3	Ref	LURAP, MAGI_u5
`ENSMUSG00000058672`	TUBB2A	Tubulin beta-2A chain	2	2	1	1,943.0	Novel	Misat_Tub_SegII, Tubulin, Tubulin_2, Tubulin_3, Tubulin_C
`ENSMUSG00000025647`	Shisa5	Protein shisa-5	3	2	1	29.4	Ref	MLANA, Shisa
`ENSMUSG00000024142`	Mlst8	Target of rapamycin complex subunit LST8	2	2	1	9.8	Ref	ANAPC4_WD40, BRICHOS, DUF3463, DUF5854, Ge1_WD40, Hira, NBCH_WD40, Nup160, WD40
`ENSMUSG00000003531`	Dgcr6	Protein DGCR6	4	2	1	144.9	Mixed	DGCR6, DUF2458
`ENSMUSG00000014856`	Tmem208	Transmembrane protein 208	5	2	1	111.6	Mixed	Halogen_Hydrol, TMEM208_SND2
`ENSMUSG00000031860`	Pbx4	Pre-B-cell leukemia transcription factor 4	2	2	1	13.7	Novel	Carbpep_Y_N, DUF6779, EF-hand_2, HTH_3, Homeobox_KN, Homeodomain, PBC, Sec15, YdaS_antitoxin
`ENSMUSG00000033392`	Clasp2	CLIP-associating protein 2	2	2	1	105.8	Ref	CLASP_N, Cas_Cas4, Cnd1, DUF4042, DUF6700, HEAT, HEAT_2, HEAT_EZ, Ipi1_N, MMS19_C, MMS19_N, ParcG, RTP1_C1, Sec7_N, TAN, TFCD_C, Tom37, Vac14_Fab1_bd
`ENSMUSG00000074865`	Znf431	Zinc finger protein 431	2	2	1	3.9	Ref	AvrPphF-ORF-2, Cu_amine_oxidN2, DUF3262, DUF3760, DUF4780, DUF5671, DUF6173, DUF6669, KRAB
`ENSMUSG00000031791`	Tmem38a	Trimeric intracellular cation channel type A	2	2	1	86.2	Novel	Metal_resist, TRIC
`ENSMUSG00000061111`	Mcrip1	Mapk-regulated corepressor-interacting protein 1	2	2	1	276.2	Ref	DUF6551, FAM195, LAP2alpha
`ENSMUSG00000075528`	Aarsd1	Alanyl-tRNA editing protein Aarsd1	2	2	1	150.8	Novel	tRNA-synt_2c, tRNA_SAD
`ENSMUSG00000027466`	Rbck1	RanBP-type and C3HC4-type zinc finger-containing protein 1	2	2	1	15.7	Novel	IBR, Rad50_zn_hook, ubiquitin, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-RanBP
`ENSMUSG00000030556`	Lrrc28	Leucine-rich repeat-containing protein 28	2	2	1	2.0	Novel	LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, SPAN-X
`ENSMUSG00000032332`	Col12a1	Collagen alpha-1(XII) chain	2	2	1	2.0	Ref	AAA_lid_4, Arylsulfotran_N, CBM_4_9, Collagen, Copine, DJ-1_PfpI, DUF1410, DUF3520, DUF4998, DUF6467, Glyco_hydro_36C, Interfer-bind, NDNF, Pur_ac_phosph_N, Toxin_R_bind_N, VWA, VWA_2, VWA_3, fn3
`ENSMUSG00000029126`	Nsg1	Neuronal vesicle trafficking-associated protein 1	3	2	1	144.9	Mixed	Calcyon, DUF1581, DUF2448, DUF535
`ENSMUSG00000000861`	Bcl11a	BCL11 transcription factor A	2	2	1	41.1	Ref	Astro_capsid_p, BSP_II, C1_4, DDHD, DNA_pol_phi, DUF2256, DUF3268, DUF4820, FAM32A, FYVE, Importin_rep_6, NOA36, Nop14, PPP4R2, RNA_pol_3_Rpc31, Ribosomal_60s, TUTase, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-H2C2_2, zf-H2C2_5, zf-HIT, zf_UBZ
`ENSMUSG00000030881`	Arfip2	Arfaptin-2	3	2	1	54.8	Ref	Arfaptin, BAR, BAR_3, Blo-t-5, TPR_MLP1_2
`ENSMUSG00000028111`	Ctsk	Cathepsin K	2	2	1	21.5	Novel	Inhibitor_I29, Peptidase_C1, Peptidase_C1_2, Peptidase_C39_2
`ENSMUSG00000056962`	Jmjd6	Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6	2	2	1	35.3	Novel	AF-4, CCDC106, Cupin_8, IMUP, JmjC, JmjC_2, SR-25
`ENSMUSG00000059810`	Rgs3	Regulator of G-protein signaling 3	2	2	1	5.9	Novel	DUF4441, RGS
`ENSMUSG00000024587`	Nars1	Asparagine--tRNA ligase, cytoplasmic	2	2	1	174.3	Ref	Cir_N, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon, tRNA_anti-like, tRNA_anti_2
`ENSMUSG00000022760`	Thap7	THAP domain-containing protein 7	2	2	1	35.3	Ref	DUF2509, OrfB_IS605, THAP, Tho2
`ENSMUSG00000037902`	Sirpa	Tyrosine-protein phosphatase non-receptor type substrate 1	2	2	1	17.6	Ref	C1-set, C2-set_2, DUF6479, I-set, Ig_2, Ig_3, MAT1-1-2, SIT, Ureide_permease, V-set, ig
`ENSMUSG00000063047`	Zfp59	Zinc finger protein 59	2	2	1	7.8	Ref	ATG13, DUF3850, HVO_2753_ZBP, HypA, KRAB, NinF, PHP, XPA_N, Zn-ribbon_8, Zn_Tnp_IS1595, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_3rep, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-met, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000022151`	Ttc33	Tetratricopeptide repeat protein 33	3	2	1	68.6	Ref	ATG14, ChAPs, TPR_1, TPR_11, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_9
`ENSMUSG00000036186`	Dipk1b	Divergent protein kinase domain 1B	2	2	1	103.8	Novel	CarbpepA_inh, PIP49_C, PIP49_N, UPF0175
`ENSMUSG00000030417`	Pdcd5	Programmed cell death protein 5	2	2	1	199.8	Ref	EF-1_beta_acid, PCI, dsDNA_bind
`ENSMUSG00000069049`	Eif2s3y	Eukaryotic translation initiation factor 2 subunit 3, Y-linked	4	2	1	11.8	Novel	Dynamin_N, G-alpha, GTP_EFTU, GTP_EFTU_D2, MMR_HSR1, MMR_HSR1_Xtn, RsgA_GTPase, eIF2_C
`ENSMUSG00000051855`	Mest	Mesoderm-specific transcript protein	2	2	1	19.6	Ref	Abhydrolase_1, Abhydrolase_6, DUF1057, Hydrolase_4, REV
`ENSMUSG00000033099`	Nol12	Nucleolar protein 12	2	2	1	7.8	Novel	ALC, FKBP_N, Nop25, Nup54_C
`ENSMUSG00000078695`	Cisd3	CDGSH iron-sulfur domain-containing protein 3, mitochondrial	2	2	1	115.6	Ref	Inhibitor_I71, Toxin_14, zf-CDGSH
`ENSMUSG00000061032`	Rrp1	Ribosomal RNA processing protein 1 homolog A	2	2	1	403.5	Ref	DNA_pol_phi, DRIM, DUF1981, DUF4391, DUF4776, MMS19_N, Nop52, PFam54_60, Tn7_Tnp_TnsA_C
`ENSMUSG00000019194`	Scn1b	Sodium channel regulatory subunit beta-1	2	2	1	144.9	Novel	DUF6264, Ig_2, Ig_3, Protocadherin, V-set, ig
`ENSMUSG00000001240`	Ramp2	Receptor activity-modifying protein 2	3	2	1	90.1	Ref	RAMP
`ENSMUSG00000063889`	Crem	cAMP-responsive element modulator	2	2	1	9.8	Ref	KfrA_N, TMCO5, Uso1_p115_C, bZIP_1, bZIP_2, bZIP_Maf, pKID
`ENSMUSG00000026201`	Stk16	Serine/threonine-protein kinase 16	5	2	1	105.8	Novel	ABC1, APH, Kdo, PK_Tyr_Ser-Thr, Pkinase, TCAD9
`ENSMUSG00000072772`	Grcc10	Protein C10	2	2	1	687.5	Ref	FAST_2, HicA_toxin, P_C10
`ENSMUSG00000022559`	Fbxl6	F-box/LRR-repeat protein 6	3	2	1	27.4	Ref	LRR_4, LRR_6, LRR_8
`ENSMUSG00000023571`	C1qtnf12	Adipolin	2	2	1	54.8	Novel	C1q, Collagen, TNF
`ENSMUSG00000004633`	CHN2	Beta-chimaerin	2	2	1	15.7	Ref	C1_1, FYVE_2, RhoGAP, SH2, zf-RING-like
`ENSMUSG00000022515`	Anks3	Ankyrin repeat and SAM domain-containing protein 3	2	2	1	13.7	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, FH2, Lebercilin, Macoilin, SAM_1, SAM_2, YlqD
`ENSMUSG00000031393`	Mecp2	Methyl-CpG-binding protein 2	2	2	1	17.6	Ref	K_channel_TID, MBD
`ENSMUSG00000064289`	Tank	TRAF family member-associated NF-kappa-B activator	2	2	1	19.6	Ref	DUF5082, OEP, PKcGMP_CC, TBD, Tetraspanin
`ENSMUSG00000027935`	Rab13	Ras-related protein Rab-13	2	2	1	11.8	Ref	AAA, AAA_16, AAA_22, AAA_25, AAA_33, AAA_7, ATP_bind_3, Arf, GTP_EFTU, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, RuvX, SRPRB, Septin, TMP-TENI, TniB
`ENSMUSG00000030869`	Ndufab1	Acyl carrier protein, mitochondrial	3	2	1	84.2	Mixed	PP-binding, PP-binding_2
`ENSMUSG00000056820`	Tsnax	Translin-associated protein X	2	2	1	266.4	Novel	Phage_GPO, Translin
`ENSMUSG00000032316`	Clk3	Dual specificity protein kinase CLK3	2	2	1	25.5	Ref	HTH_48, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000025512`	Chid1	Chitinase domain-containing protein 1	2	2	1	3.9	Ref	CoA_binding_2, Glyco_hydro_18, Glyco_hydro_85
`ENSMUSG00000028411`	Aptx	Aprataxin	2	2	1	45.0	Ref	CBP_BcsO, DcpS_C, FHA, FHA_2, Glycoamylase, HIT, TAF8_C, zf-AN1, zf-C2H2_4, zf-C2H2_6, zf-C2HE
`ENSMUSG00000062352`	Itgb1bp1	Integrin beta-1-binding protein 1	2	2	1	19.6	Novel	DHOase, ICAP-1_inte_bdg, PID, YCII
`ENSMUSG00000073411`	H2-D1	H-2 class I histocompatibility antigen, D-B alpha chain	2	2	1	50.9	Ref	C1-set, C2-set_2, Ig_2, Ig_3, MHC_I, MHC_I_C, Omp85, ig
`ENSMUSG00000022415`	Syngr1	Synaptogyrin-1	2	2	1	239.0	Ref	Col_cuticle_N, DUF4064, DUF805, Fig1, MARVEL, Ost5
`ENSMUSG00000025290`	RPS24	Small ribosomal subunit protein eS24	3	2	1	1,143.9	Ref	Ribosomal_S24e
`ENSMUSG00000028729`	Ebna1bp2	Probable rRNA-processing protein EBP2	2	2	1	15.7	Ref	Ebp2, Sec39, eIF3_N
`ENSMUSG00000036854`	Hspb6	Heat shock protein beta-6	2	2	1	17.6	Novel	ArsA_HSP20, Crystallin, HSP20, SelB-wing_2
`ENSMUSG00000047084`	Ngrn	Neugrin	2	2	1	103.8	Ref	Dermcidin, MRP-L20, Neugrin, Ribosomal_L29, UPF0122
`ENSMUSG00000026921`	Egfl7	Epidermal growth factor-like protein 7	2	2	1	39.2	Mixed	EGF, EGF_2, EGF_CA, EMI, SlyX
`ENSMUSG00000059436`	Max	Protein max	2	2	1	41.1	Ref	ATG16, Csm1_N, DUF4140, DUF4763, DUF5595, DUF5716, DivIC, FapA, Filament, HALZ, HLH, JAKMIP_CC3, Jnk-SapK_ap_N, SLX9, SlyX, V_ATPase_I, ZapB, bZIP_2
`ENSMUSG00000002329`	Mdp1	Magnesium-dependent phosphatase 1	2	2	1	56.8	Ref	ABM, Acid_PPase, HAD_2, Hydrolase, NIF
`ENSMUSG00000072946`	Ptgr2	Prostaglandin reductase 2	4	2	1	19.6	Ref	ADH_N_2, ADH_zinc_N, ADH_zinc_N_2
`ENSMUSG00000072115`	Ang	Angiogenin	2	2	1	9.8	Ref	Daz, RnaseA
`ENSMUSG00000026240`	Cops7b	COP9 signalosome complex subunit 7b	3	2	1	3.9	Ref	Band_3_cyto, CSN7a_helixI, DUF4464, PCI
`ENSMUSG00000034563`	Ccpg1	Cell cycle progression protein 1	2	2	1	27.4	Ref	Csm1_N, DHR-2_Lobe_C, DUF1664, DUF948, YtxH
`ENSMUSG00000024830`	Rps6kb2	Ribosomal protein S6 kinase beta-2	3	2	1	2.0	Mixed	DUF6499, PK_Tyr_Ser-Thr, Pkinase, Pkinase_C
`ENSMUSG00000003873`	Bax	Apoptosis regulator BAX	3	2	1	121.4	Novel	BID, Bcl-2, Bclx_interact, CF222
`ENSMUSG00000022681`	Ntan1	Protein N-terminal asparagine amidohydrolase	7	2	1	107.7	Novel	FIST, N_Asn_amidohyd
`ENSMUSG00000038005`	Hpf1	Histone PARylation factor 1	5	2	1	146.9	Mixed	DUF2228, DUF6027
`ENSMUSG00000025421`	Hdhd2	Haloacid dehalogenase-like hydrolase domain-containing protein 2	5	2	1	127.3	Ref	Acid_phosphat_B, DUF3155, HAD_2, Hydrolase, Hydrolase_6, Hydrolase_like
`ENSMUSG00000041912`	Tdrkh	Tudor and KH domain-containing protein	2	2	1	13.7	Ref	KH_1, KH_2, KH_4, KH_5, MOEP19, Ribosomal_S12, TUDOR
`ENSMUSG00000010755`	Cars1	Cysteine--tRNA ligase, cytoplasmic	3	2	1	35.3	Ref	DUF2175, tRNA-synt_1, tRNA-synt_1c, tRNA-synt_1e, tRNA-synt_1g
`ENSMUSG00000054690`	Emcn	Endomucin	2	2	1	2.0	Ref	DUF2207, DUF6479, Endomucin, IPP-2, Psg1, RIFIN, Stevor
`ENSMUSG00000024369`	Nelfe	Negative elongation factor E	3	2	1	15.7	Ref	Nup35_RRM_2, RPA43_OB, RRM_1, RRM_occluded
`ENSMUSG00000026553`	Copa	Coatomer subunit alpha	2	2	1	39.2	Ref	ANAPC4_WD40, COPI_C, Coatomer_WDAD, NBCH_WD40, Noda_Vmethyltr, Nup160, TPR_14, WD40
`ENSMUSG00000031371`	Haus7	HAUS augmin-like complex subunit 7	2	2	1	33.3	Ref	AATF-Che1, FlgN, Nsp1_C, Plant_NMP1, YabA
`ENSMUSG00000024012`	Mtch1	Mitochondrial carrier homolog 1	6	2	1	92.1	Ref	Mito_carr, YdjM
`ENSMUSG00000031708`	Tecr	Very-long-chain enoyl-CoA reductase	3	2	1	2,029.2	Ref	Steroid_dh
`ENSMUSG00000021519`	Mterf3	Transcription termination factor 3, mitochondrial	2	2	1	49.0	Ref	Pex14_N, mTERF
`ENSMUSG00000022043`	Trim35	E3 ubiquitin-protein ligase TRIM35	2	2	1	66.6	Ref	ATG16, Baculo_IE-1, CCDC90-like, DUF2203, DUF4200, Fez1, PRY, Prok-RING_4, SPRY, zf-B_box, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX
`ENSMUSG00000048076`	ARF1	ADP-ribosylation factor 1	2	2	1	552.3	Ref	6PF2K, Arf, DO-GTPase2, DUF1806, G-alpha, Gtr1_RagA, MMR_HSR1, Ras, Roc, SRPRB
`ENSMUSG00000035203`	Epn1	Epsin-1	2	2	1	50.9	Novel	ANTH, DUF658, ENTH, UIM, VHS
`ENSMUSG00000029199`	Lias	Lipoyl synthase, mitochondrial	2	2	1	56.8	Ref	Glyco_transf_4, LIAS_N, ROS_MUCR, Radical_SAM, SVWC
`ENSMUSG00000029657`	Hsph1	Heat shock protein 105 kDa	2	2	1	188.0	Ref	CCDC90-like, CHAD, F-box-like_2, FGGY_C, FtsA, HSP70, MreB_Mbl, Spectrin
`ENSMUSG00000030850`	Ate1	Arginyl-tRNA--protein transferase 1	2	2	1	2.0	Ref	ATE_C, ATE_N, DUF945
`ENSMUSG00000031072`	Lto1	Protein LTO1 homolog	2	2	1	27.4	Ref	FAD_binding_1, Yae1_N
`ENSMUSG00000067873`	Htatsf1	17S U2 SnRNP complex component HTATSF1	2	2	1	43.1	Ref	Nup35_RRM, RRM_1, RRM_7
`ENSMUSG00000058587`	Tmod3	Tropomodulin-3	2	2	1	31.3	Ref	LRR_6, Tropomodulin, Usher
`ENSMUSG00000030374`	Strn4	Striatin-4	2	2	1	19.6	Novel	ANAPC4_WD40, CENP-H, Ge1_WD40, NBCH_WD40, Nucleoporin_N, Nup160, Striatin, WD40
`ENSMUSG00000078919`	Dpm1	Dolichol-phosphate mannosyltransferase subunit 1	3	2	1	43.1	Ref	Glyco_tranf_2_2, Glyco_tranf_2_3, Glyco_tranf_2_4, Glyco_transf_21, Glycos_transf_2
`ENSMUSG00000045867`	Cradd	Death domain-containing protein CRADD	2	2	1	13.7	Ref	CARD, Death, Gam, TadE, Tmemb_18A
`ENSMUSG00000018196`	Glrx2	Glutaredoxin-2, mitochondrial	2	2	1	25.5	Ref	DSBA, DUF2153, DUF5935, GST_N_3, Glutaredoxin, Thioredoxin_2, Thioredoxin_3
`ENSMUSG00000029048`	Rer1	Protein RER1	2	2	1	86.2	Ref	DUF2208, Rer1, SEP
`ENSMUSG00000039285`	Azi2	5-azacytidine-induced protein 2	2	2	1	7.8	Novel	CENP-F_leu_zip, Cnn_1N, Csm1_N, DMPK_coil, DUF1090, DUF1319, DUF1967, DUF4407, DivIC, FlaC_arch, HIP1_clath_bdg, Herpes_BLRF2, PRKG1_interact, Prefoldin_2, SurA_N, TBD, TMF_TATA_bd, TPR_MLP1_2, UPF0242, YlqD, bZIP_2
`ENSMUSG00000024160`	Spsb3	SPRY domain-containing SOCS box protein 3	3	2	1	82.3	Ref	SOCS_box, SPRY
`ENSMUSG00000030058`	Copg1	Coatomer subunit gamma-1	2	2	1	203.7	Ref	Adaptin_N, Arm, COP-gamma_platf, Cm_res_leader, Cnd1, Cnd3, Coatomer_g_Cpla, HEAT, HEAT_2, HEAT_EZ, HEAT_PBS, MOR2-PAG1_C, PDS5, UNC45-central
`ENSMUSG00000031347`	Cetn2	Centrin-2	2	2	1	66.6	Ref	Asp-B-Hydro_N, CNNM, Caleosin, DUF4601, DUF6694, Dockerin_1, EF-hand_1, EF-hand_11, EF-hand_14, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, Fin, HerA_C, LETM1_RBD, RTP, SRI, SUB1_ProdP9, SurA_N_2, SurA_N_3
`ENSMUSG00000055692`	Tmem191	Transmembrane protein 191	2	2	1	33.3	Novel	ATG16, CheZ, DUF1664, DUF4407, FUSC, GAS, KASH_CCD, OmpH, RNase_Y_N, Sec20, Spc24, Spc7, TMEM191C, pRN1_helical
`ENSMUSG00000034930`	Rtkn	Rhotekin	4	2	1	5.9	Novel	Anillin, HR1, PH
`ENSMUSG00000054477`	Kcnn2	Small conductance calcium-activated potassium channel protein 2	2	2	1	19.6	Novel	AATF-Che1, CaMBD, DUF4988, DUF6775, Desmo_N, IRK, Ion_trans_2, Med9, PMP1_2, Prefoldin_2, Prominin, SK_channel, SlyX, YvrJ
`ENSMUSG00000027804`	Ppid	Peptidyl-prolyl cis-trans isomerase D	2	2	1	92.1	Novel	ANAPC3, Pro_isomerase, TPR_1, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000015120`	UBE2I	SUMO-conjugating enzyme UBC9	8	2	1	107.7	Ref	Prok-E2_B, UQ_con
`ENSMUSG00000026790`	Odf2	Outer dense fiber protein 2	2	2	1	5.9	Ref	ADIP, Crescentin, Filament, GAS, Mt_ATP-synt_D, NAT, TMF_DNA_bd, TMF_TATA_bd
`ENSMUSG00000034194`	R3hcc1	R3H and coiled-coil domain-containing protein 1	2	2	1	29.4	Ref	BUD22, CSRNP_N, DUF4407, Dicty_REP, NCBP3, R3H, SDA1
`ENSMUSG00000035478`	Mbd3	Methyl-CpG-binding domain protein 3	4	2	1	37.2	Mixed	MBD, MBD_C, MBDa, NUMOD1, RasGAP
`ENSMUSG00000048550`	Thnsl1	Threonine synthase-like 1	2	2	1	0.0	Ref	AAA, AAA_17, AAA_18, AAA_19, AAA_22, AAA_24, AAA_28, AAA_33, Acid_PPase, CPT, Cytidylate_kin, IstB_IS21, Mg_chelatase, PALP, RNA12, Rad17, RuvB_N, SKI, Thr_synth_N, Zeta_toxin, bpMoxR, dNK
`ENSMUSG00000031948`	Kars1	Lysine--tRNA ligase	3	2	1	56.8	Ref	DUF4407, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon
`ENSMUSG00000035278`	Plekhj1	Pleckstrin homology domain-containing family J member 1	2	2	1	160.6	Novel	PH, PH_13, PH_16, Pinin_SDK_N
`ENSMUSG00000005986`	Ankrd13d	Ankyrin repeat domain-containing protein 13D	2	2	1	39.2	Novel	Ank, Ank_2, Ank_3, Ank_4, Ank_5, GPCR_chapero_1, UIM
`ENSMUSG00000041199`	Rpusd1	RNA pseudouridylate synthase domain-containing protein 1	4	2	1	90.1	Ref	PseudoU_synth_2
`ENSMUSG00000051950`	B3glct	Beta-1,3-glucosyltransferase	2	2	1	0.0	Novel	DUF3939, DUF604, Fringe, Galactosyl_T, Glycos_transf_2
`ENSMUSG00000026797`	STXBP1	Syntaxin-binding protein 1	2	2	1	432.9	Ref	DUF5344, FRB_dom, Sec1, YlbD_coat
`ENSMUSG00000047635`	Mtrfr	Mitochondrial translation release factor in rescue	2	2	1	37.2	Ref	DUF3095, PDCD7, RF-1
`ENSMUSG00000075467`	Dnlz	DNL-type zinc finger protein	2	2	1	7.8	Ref	DUF6112, DUF6510, HypA, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-CSL, zf-DNL
`ENSMUSG00000021773`	Comtd1	Catechol O-methyltransferase domain-containing protein 1	2	2	1	19.6	Ref	DNA_binding_1, MTS, Methyltransf_18, Methyltransf_24, Methyltransf_25, Methyltransf_3, Methyltransf_31, PCMT
`ENSMUSG00000068921`	Dap3	Small ribosomal subunit protein mS29	4	2	1	29.4	Mixed	AAA_25, DAP3, DUF1841, DUF3944
`ENSMUSG00000047547`	Cltb	Clathrin light chain B	2	2	1	266.4	Ref	CENP-Q, Clathrin_lg_ch, DUF6161, GED
`ENSMUSG00000022037`	Clu	Clusterin	3	2	1	1,100.8	Mixed	Clusterin, LXG, Laminin_II, Mod_r, Mt_ATP-synt_B, SOAR, Syntaxin_2, T3SS_needle_E, YlqD, pAdhesive_17
`ENSMUSG00000053801`	Grwd1	Glutamate-rich WD repeat-containing protein 1	2	2	1	7.8	Ref	ANAPC4_WD40, CAF1C_H4-bd, CENP-B_dimeris, IKI3, Voldacs, WD40
`ENSMUSG00000025867`	CPLX2	Complexin-2	2	2	1	642.4	Novel	AP-5_subunit_s1, Borrelia_P83, DDHD, DUF2838, DUF4407, MDM10, RNA_polI_A34, RR_TM4-6, Spt5_N, Synaphin, VIT1
`ENSMUSG00000007987`	Ift22	Intraflagellar transport protein 22 homolog	2	2	1	25.5	Ref	AAA, Arf, FMN_red, G-alpha, GTP_EFTU, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, SRPRB
`ENSMUSG00000029363`	Rfc5	Replication factor C subunit 5	2	2	1	7.8	Ref	AAA, AAA_11, AAA_14, AAA_16, AAA_19, AAA_22, AAA_24, AAA_25, AAA_3, AAA_30, AAA_5, AAA_7, AAA_assoc_2, ATPase_2, DEAD, DNA_pol3_delta, DNA_pol3_delta2, DUF815, Mg_chelatase, PhoH, RNA_helicase, Rad17, Rep_fac_C, ResIII, RuvB_N, T2SSE, TIP49, TniB, bpMoxR
`ENSMUSG00000029712`	ACTL6B	Actin-like protein 6B	4	2	1	170.4	Novel	Actin, MobA_MobL, MreB_Mbl
`ENSMUSG00000037236`	Matr3	Matrin-3	2	2	1	119.5	Ref	CCDC53, CTU2, FAM60A, NPR3, Pneumo_att_G, RRM_1, RRM_5, U62_UL91, Utp14, zf-C2H2_jaz, zf-U1
`ENSMUSG00000039357`	Fut11	GDP-fucose protein O-fucosyltransferase 4	2	2	1	3.9	Ref	Glyco_tran_10_N, Glyco_transf_10
`ENSMUSG00000030824`	Nucb1	Nucleobindin-1	2	2	1	39.2	Ref	EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, RNase_Y_N
`ENSMUSG00000020912`	Krt12	Keratin, type I cytoskeletal 12	2	2	1	43.1	Novel	ADIP, APC_N_CC, ATG16, Bacillus_HBL, COG2, DUF1664, DUF4407, DivIC, ERM_helical, Filament, LPP, Mod_r, SKA1, Sec2p, Sec34, Tweety, Uso1_p115_C, V_ATPase_I, YabA, zf-C4H2
`ENSMUSG00000042078`	Svop	Synaptic vesicle 2-related protein	2	2	1	37.2	Novel	DUF1673, DUF2070, MFS_1, MFS_2, Sugar_tr
`ENSMUSG00000029359`	Tesc	Calcineurin B homologous protein 3	2	2	1	280.1	Ref	EF-hand_1, EF-hand_2, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, GIT_CC, M16C_assoc
`ENSMUSG00000015126`	Tsr3	18S rRNA aminocarboxypropyltransferase	5	2	1	94.0	Ref	RLI, Ribo_biogen_C
`ENSMUSG00000029924`	Slc37a3	Sugar phosphate exchanger 3	2	2	1	15.7	Ref	DUF485, MFS_1, OATP, SpoIIIAC, Sugar_tr, TRI12
`ENSMUSG00000055850`	Rnf181	E3 ubiquitin-protein ligase RNF181	2	2	1	184.1	Novel	BIR, FANCL_C, PHD, Prok-RING_1, Prok-RING_4, RINGv, Rad50_zn_hook, UBZ_FAAP20, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000010095`	Slc3a2	Amino acid transporter heavy chain SLC3A2	3	2	1	96.0	Ref	Alpha-amylase, DUF3459, Malt_amylase_C, SLC3A2_N, YlxR
`ENSMUSG00000054021`	Sirt5	NAD-dependent protein deacylase sirtuin-5, mitochondrial	2	2	1	19.6	Ref	GlgS, PNTB, RseA_N, SIR2
`ENSMUSG00000020629`	Adi1	Acireductone dioxygenase	2	2	1	101.9	Ref	ARD, AraC_binding, Cupin_1, Cupin_2, Cupin_3, Cupin_8, DMSP_lyase, DUF4078, JmjC_2
`ENSMUSG00000056737`	Capg	Macrophage-capping protein	2	2	1	11.8	Ref	Gelsolin, TB
`ENSMUSG00000121345`	NSFL1C	NSFL1 cofactor p47	3	2	1	15.7	Ref	Cadherin, DUF179, UBA_4, zf-U1
`ENSMUSG00000037720`	Tmem33	Transmembrane protein 33	4	2	1	13.7	Ref	TMEM33_Pom33
`ENSMUSG00000031781`	Ciapin1	Anamorsin	2	2	1	13.7	Novel	BBS2_C, CIAPIN1, Methyltransf_11, Methyltransf_12, Methyltransf_23, Methyltransf_25, Methyltransf_29, Methyltransf_31, RGS12_usC, Tat
`ENSMUSG00000030714`	Sgf29	SAGA-associated factor 29	2	2	1	9.8	Ref	DUF1325, Filament, TUDOR, Tudor_3
`ENSMUSG00000020083`	Fam241b	Protein FAM241B	3	2	1	70.5	Ref	DUF4605
`ENSMUSG00000026096`	Osgepl1	tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial	2	2	1	17.6	Ref	BcrAD_BadFG, CheC, NADH_4Fe-4S, TsaD
`ENSMUSG00000110444`	Znf431	Zinc finger protein 431	2	2	2	5.9	Ref	BHD_1, C1_4, Cas6_N, FRB_dom, GATA, KRAB, NCD1, Tna_leader, Utp12, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2HC_2, zf-H2C2_2, zf-trcl, zf_C2H2_13, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000074899`	SPTBN5	Spectrin beta chain, non-erythrocytic 5	2	2	2	0.0	Novel	ABC_tran_CTD, AHSP, HEAT_PBS, Spectrin
`ENSMUSG00000050708`	Ftl1	Ferritin light chain 1	2	2	3	235.0	Ref	ANAPC4, Ferritin
`ENSMUSG00000031585`	Gtf2e2	General transcription factor IIE subunit 2	2	2	4	25.5	Ref	PhoQ_Sensor, TFA2_Winged_2, TFIIE_beta
`ENSMUSG00000040462`	Os9	Protein OS-9	2	2	4	111.6	Ref	PRKCSH, PRKCSH_1, RRN3, SNAP-25
`ENSMUSG00000033728`	Lrrc14	Leucine-rich repeat-containing protein 14	2	2	4	9.8	Novel	LRR_1, LRR_4, LRR_6, LRR_8, MmeI_TRD, RIIa, zf-3CxxC
`ENSMUSG00000018858`	Mrpl58	Large ribosomal subunit protein mL62	3	2	4	64.6	Ref	NOG1_N, RF-1, pEK499_p136
`ENSMUSG00000021039`	Snw1	SNW domain-containing protein 1	3	2	4	66.6	Novel	SIR4_SID, SKIP_SNW
`ENSMUSG00000024352`	Spata24	Spermatogenesis-associated protein 24	2	2	4	15.7	Ref	DUF4335, DUF4464, ERM_helical, Fez1, Fib_alpha, LMBR1, Lebercilin, SPATA24
`ENSMUSG00000028634`	—	—	2	2	4	74.4	Novel	Activin_recp, SIP1
`ENSMUSG00000027593`	Raly	RNA-binding protein Raly	2	2	4	107.7	Ref	Filament_head, GRP, Herpes_capsid, RRM_1, RRM_5, RhlB
`ENSMUSG00000071454`	Dtnb	Dystrobrevin beta	2	2	4	9.8	Novel	CRISPR_Cse2, DUF4407, DUF4988, EF-hand_2, EF-hand_3, FUSC, FapA, GET2, MerR-DNA-bind, SOGA, Seryl_tRNA_N, YkyA, ZZ
`ENSMUSG00000020327`	Fgf22	Fibroblast growth factor 22	2	2	4	2.0	Ref	DUF4529, FGF, Fascin
`ENSMUSG00000031858`	Mau2	MAU2 chromatid cohesion factor homolog	2	2	4	7.8	Ref	AmyA-A_glucT_m, Cohesin_load, HemY_N, Ribosomal_60s, TPR_1, TPR_10, TPR_12, TPR_2, TPR_8, TPR_MalT
`ENSMUSG00000004892`	Bcan	Brevican core protein	2	2	4	88.1	Novel	EGF, EGF_2, EGF_3, EGF_CA, I-set, Ig_2, Ig_3, Izumo-Ig, Laminin_EGF, Lectin_C, Sushi, V-set, Xlink, hEGF, ig
`ENSMUSG00000039148`	Sart1	U4/U6.U5 tri-snRNP-associated protein 1	2	2	4	3.9	Ref	HIND, SART-1, T3SS_needle_E
`ENSMUSG00000079426`	ARPC4	Actin-related protein 2/3 complex subunit 4	2	2	4	190.0	Ref	ARPC4, Cmr7A, GPS2_interact, Sm_like
`ENSMUSG00000063765`	Chadl	Chondroadherin-like protein	2	2	4	33.3	Ref	LRRCT, LRRNT, LRR_1, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9
`ENSMUSG00000012126`	Ubxn11	UBX domain-containing protein 11	2	2	4	5.9	Novel	DUF1635, DUF883, Exonuc_VII_L, FAM76, MnmE_helical, OmpH, SEP, TMPIT, TRAF_BIRC3_bd, UBX
`ENSMUSG00000064181`	Rab3ip	Rab-3A-interacting protein	2	2	4	39.2	Ref	ATG16, Borrelia_P83, Crescentin, DUF5082, FAM76, FapA, HMMR_N, Sec2p, TMF_TATA_bd, TPR_MLP1_2, UPF0242
`ENSMUSG00000039536`	Stau1	Double-stranded RNA-binding protein Staufen homolog 1	4	2	4	25.5	Ref	DND1_DSRM, LPP20, Staufen_C, dsrm
`ENSMUSG00000057113`	Npm1	Nucleophosmin	2	2	4	64.6	Ref	CDC45, NOA36, NPM1-C, Nop14, Nucleoplasmin, SDA1, TFIIF_alpha, YL1
`ENSMUSG00000022193`	Psmb5	Proteasome subunit beta type-5	2	2	4	1,145.8	Ref	DUF3991, Proteasome
`ENSMUSG00000022536`	Glyr1	Cytokine-like nuclear factor N-PAC	2	2	4	13.7	Ref	2-Hacid_dh_C, 3HCDH_N, AT_hook, ApbA, CholecysA-Rec_N, F420_oxidored, NAD_Gly3P_dh_N, NAD_binding_11, NAD_binding_2, PWWP, Sacchrp_dh_NADP, Shikimate_DH
`ENSMUSG00000039771`	POLR2J	DNA-directed RNA polymerase II subunit RPB11	2	2	4	199.8	Ref	RNA_pol_L, RNA_pol_L_2, SHNi-TPR
`ENSMUSG00000062753`	Smim29	Small integral membrane protein 29	2	2	4	52.9	Ref	ABC2_membrane_3, BCCT, COX14, CcmH, DUF2371, DUF3267, DUF3619, DUF4245, Hol_Tox, PaREP1
`ENSMUSG00000121778`	Ube2v1	Ubiquitin-conjugating enzyme E2 variant 1	4	2	4	470.1	Ref	UQ_con
`ENSMUSG00000024146`	Cript	Cysteine-rich PDZ-binding protein	2	2	4	274.2	Ref	Cas_CXXC_CXXC, Cript, DUF1272, DUF2039, DZR_2, Fin, Gin, PLATZ, Rhodanese_C, zf-RING_9, zinc_ribbon_2
`ENSMUSG00000055430`	Nap1l5	Nucleosome assembly protein 1-like 5	2	2	4	360.4	Novel	ANAPC15, Ax_dynein_light, Dicty_REP, HrpB7, Mst1_SARAH, NAP, OmpH, Phage_Mu_Gam, Voldacs
`ENSMUSG00000029146`	Snx17	Sorting nexin-17	2	2	4	125.4	Ref	Cation_ATPase_N, DUF2777, FERM_M, Nod1, PX, RA, Ras_bdg_2, SNX17_FERM_C
`ENSMUSG00000026414`	Tnnt2	Troponin T, cardiac muscle	2	2	4	7.8	Novel	DUF4741, PLN_propep, Troponin
`ENSMUSG00000003411`	Rab3b	Ras-related protein Rab-3B	3	2	4	45.0	Ref	AAA_16, AAA_24, Arf, FeoB_N, Fin, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB
`ENSMUSG00000032966`	Fkbp1a	Peptidyl-prolyl cis-trans isomerase FKBP1A	2	2	4	1,627.7	Ref	FKBP_C, Glyoxalase_4
`ENSMUSG00000014873`	Surf2	Surfeit locus protein 2	2	2	4	43.1	Novel	PAE, SURF2
`ENSMUSG00000004631`	Sgce	Epsilon-sarcoglycan	3	2	4	29.4	Ref	Sarcoglycan_2
`ENSMUSG00000025138`	Sirt7	NAD-dependent protein deacetylase sirtuin-7	2	2	4	13.7	Ref	Glyco_transf_61, SIR2, TPP_enzyme_M
`ENSMUSG00000092607`	Scnm1	Sodium channel modifier 1	2	2	4	31.3	Ref	SCNM1_acidic, zf-SCNM1
`ENSMUSG00000026018`	Ica1l	Islet cell autoantigen 1-like protein	2	2	4	3.9	Ref	Arfaptin, Glyco_hydro_15, ICA69, Nuf2_DHR10-like
`ENSMUSG00000032551`	—	Uncharacterized protein KIAA1143 homolog	2	2	4	15.7	Ref	DUF4604, DUF4848, Peptidase_S49_N
`ENSMUSG00000118125`	Fam89b	Leucine repeat adapter protein 25	2	2	4	13.7	Novel	Auto_anti-p27, LURAP, MAGI_u5, OrfB_Zn_ribbon
`ENSMUSG00000032527`	Pccb	Propionyl-CoA carboxylase beta chain, mitochondrial	2	2	4	21.5	Ref	Carboxyl_trans, Lys_Orn_oxgnase, MdcE
`ENSMUSG00000021606`	Ndufs6	NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial	2	2	4	197.8	Ref	Chitin_synth_2, Thiolase_C, zf-CHCC
`ENSMUSG00000019210`	Atp6v1e1	V-type proton ATPase subunit E 1	2	2	4	1,725.6	Novel	ATP-synt_B, DUF4288, Folliculin, NifW, O-antigen_lig, Par3_HAL_N_term, vATP-synt_E
`ENSMUSG00000020832`	Eral1	GTPase Era, mitochondrial	2	2	4	19.6	Ref	AAA_16, AAA_28, AIG1, Arf, Dynamin_N, FeoB_N, G-alpha, GTP_EFTU, KH_1, KH_2, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB, TniB, cobW
`ENSMUSG00000050947`	Amigo1	Amphoterin-induced protein 1	2	2	4	27.4	Ref	I-set, Ig_2, Ig_3, LRR_1, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9, Reticulon, V-set, ig
`ENSMUSG00000001018`	Snapin	SNARE-associated protein Snapin	2	2	4	66.6	Ref	CATRA-C, DUF5798, E2F_TDP, Laminin_II, Snapin_Pallidin
`ENSMUSG00000024109`	Nrxn1	Neurexin-1-beta	2	2	4	5.9	Ref	CD34_antigen, COX14, Colicin_V, DUF202, DUF3687, Orf78, Syndecan
`ENSMUSG00000034429`	ZNF707	Zinc finger protein 707	2	2	4	33.3	Ref	BolA, C1_4, CpXC, DpnI, HypA, KRAB, Zn-ribbon_8, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_jaz, zf-H2C2_2, zf-LYAR, zf-RING_7, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000057147`	Dph6	Diphthine--ammonia ligase	2	2	4	2.0	Ref	Diphthami_syn_2, tRNA_Me_trans
`ENSMUSG00000001383`	ZMAT2	Zinc finger matrin-type protein 2	2	2	4	113.6	Novel	Cgr1, DDRGK, DUF4250, DUF6277, FOXP-CC, SNRNP27, TUTase, Tra1_central, zf-C2H2, zf-C2H2_jaz, zf-U1, zf-met
`ENSMUSG00000027574`	Nkain4	Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4	2	2	4	139.1	Ref	12TM_1, DUF1003, NKAIN
`ENSMUSG00000003235`	Eif2b5	Translation initiation factor eIF2B subunit epsilon	2	2	4	21.5	Novel	DUF4954, Fucokinase, Hexapep, Hexapep_2, K_channel_TID, W2
`ENSMUSG00000025735`	Rhbdl1	Rhomboid-related protein 1	2	2	4	62.7	Novel	DUF5580, HPP, Rhomboid
`ENSMUSG00000049600`	Zbtb45	Zinc finger and BTB domain-containing protein 45	2	2	4	25.5	Novel	BTB, DUF2256, HypA, LIM, Zn-ribbon_8, Zn_Tnp_IS1595, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-Di19, zf-H2C2_2, zf-met, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000056228`	Cars2	Probable cysteine--tRNA ligase, mitochondrial	2	2	4	9.8	Ref	DALR_2, tRNA-synt_1, tRNA-synt_1e, tRNA-synt_1f, tRNA-synt_1g
`ENSMUSG00000059772`	Slx1b	Structure-specific endonuclease subunit SLX1	2	2	4	5.9	Ref	FANCL_C, GIY-YIG, PHD, zf-RING-like, zinc-ribbons_6
`ENSMUSG00000028066`	Pmf1	Polyamine-modulated factor 1	2	2	4	11.8	Ref	BLOC1_2, BRI3BP, DUF16, DUF1664, DUF4618, Ku_PK_bind, MnmE_helical, Mod_r, Nnf1, OmpH, QueF, RmuC, SYCP2_ARLD, SlyX, TSC22, TetR_C_4, Uso1_p115_C, Wtap, betaPIX_CC
`ENSMUSG00000025503`	Taldo1	Transaldolase	2	2	4	448.5	Ref	TAL_FSA, Totivirus_coat
`ENSMUSG00000058833`	Rex1bd	Required for excision 1-B domain-containing protein	2	2	4	276.2	Ref	DNA_repr_REX1B, EST1, FliJ, MscS_porin
`ENSMUSG00000033972`	Zfp54	Zinc finger protein 54	2	2	4	3.9	Novel	C1_4, KRAB, tRNA-synt_1_2, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_jaz, zf-H2C2_2, zf-met
`ENSMUSG00000063480`	SNU13	NHP2-like protein 1	2	2	4	49.0	Novel	DUF4094, Fes1, PELOTA_1, RNase_P_pop3, Ribosomal_L7Ae
`ENSMUSG00000034484`	Snx2	Sorting nexin-2	2	2	4	45.0	Novel	4HB_MCP_1, Allexi_40kDa, Arfaptin, BAR_3_WASP_bdg, BLOC1_2, DUF1870, DUF3775, DUF4618, DUF4795, Dynamin_N, Exonuc_VII_L, FAM92, Fez1, Fib_alpha, FliD_N, GrpE, HMMR_N, HR1, PX, Prefoldin_2, Sec34, Sorting_nexin, Vps5
`ENSMUSG00000033720`	Sfxn5	Sideroflexin-5	3	2	4	64.6	Ref	DUF1980, SFXNs
`ENSMUSG00000020496`	Rnf187	E3 ubiquitin-protein ligase RNF187	2	2	4	278.1	Novel	DUF1801, FYDLN_acid, Fer2, Prok-RING_4, zf-B_box, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-C3HC4_5, zf-Nse, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000026424`	Gpr37l1	G-protein coupled receptor 37-like 1	2	2	4	262.5	Novel	7tm_1, Prominin

DV Enrichment by Expression Level (CPM from upstream transcriptomics)

Baseline DV Rate

8.9%

745 / 8,382 genes

Peak DV Rate

14.4%

High (50-200) bin

Peak Enrichment

1.6×

vs baseline

Chi-square trend

p < 0.001

Expression Bin	Total Genes	DV Genes	DV %	Fold vs Baseline
Silent (<1) CPM	468	10	2.1%	0.2×
Low (1-10) CPM	2,942	138	4.7%	0.5×
Moderate (10-50) CPM	2,741	279	10.2%	1.1×
High (50-200) CPM	1,500	216	14.4%	1.6×
Very high (>=200) CPM	731	102	14.0%	1.6×

Bar height shows the percentage of genes that are domain-variant within each expression bin. Dashed line = overall DV rate (8.9%). If DV enrichment increases monotonically with expression, ascertainment bias (more reads = more isoform discovery) is the dominant driver. Enrichment above baseline at specific expression tiers may indicate biological enrichment beyond the detection effect. CPM from 510,546 mRNA reads (0.1% rRNA excluded).

ORF Type Distribution

NMD Prediction by ORF Type

NMD Candidates by Evidence Tier

NMD Expression Validation (CPM from upstream transcriptomics)

NMD Expression Rate

97.5%

307 / 315

Non-NMD Expression Rate

96.2%

10,670 / 11,087

Fold Difference

1.0×

non-NMD / NMD

Tier 1 NMD Expression

96.3%

105 / 109

NMD Candidates (n=315)

Non-NMD (n=11,087)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual transcript CPM values are overlaid as jittered points. NMD candidates are expressed at 97.5% (median 5.9 CPM) vs 96.2% (median 11.8 CPM) for non-NMD — no evidence of NMD-mediated degradation.

Output Files

blastp.with_title.tsv HPC 1.7 MB gene_functional_summary.tsv HPC 336.5 KB isoform_annotations.tsv HPC 2.5 MB job.yml HPC 608 B pfam.domtblout HPC 25.4 MB results_summary.json HPC 1382.4 KB

Provenance

Execution	Expression quantification summary
Completed	2026-03-01T04:20:35+00:00

RO-Crate 1.1 Workflow RO-Crate 1.0 FAIR

This analysis is packaged as a Research Object Crate with machine-readable provenance and FAIR metadata.

RO-Crate Metadata (JSON-LD)

Show/hide raw JSON-LD

{
    "@context": [
        "https://w3id.org/ro/crate/1.1/context",
        {
            "bioschemas": "https://bioschemas.org/"
        }
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                    "@id": "https://w3id.org/ro/crate/1.1"
                },
                {
                    "@id": "https://w3id.org/workflowhub/workflow-ro-crate/1.0"
                }
            ]
        },
        {
            "@id": "./",
            "@type": "Dataset",
            "name": "ONT Isoform Functional Annotation \u2014 Run #51",
            "description": "Pfam domain search, SwissProt homology, NMD prediction, and functional diversity classification for ONT transcript isoforms.",
            "datePublished": "2026-03-01",
            "license": {
                "@id": "https://creativecommons.org/licenses/by/4.0/"
            },
            "mainEntity": {
                "@id": "ont_isoform_annotation.cwl"
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                    "@id": "isoform_annotations.tsv"
                },
                {
                    "@id": "gene_functional_summary.tsv"
                },
                {
                    "@id": "blastp.with_title.tsv"
                },
                {
                    "@id": "pfam.domtblout"
                },
                {
                    "@id": "results_summary.json"
                },
                {
                    "@id": "summary_extractor.py"
                }
            ],
            "mentions": [
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                {
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                    "@id": "#process-115"
                },
                {
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            "@type": [
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            "name": "ONT Isoform Functional Annotation",
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        {
            "@id": "#protocol-42",
            "@type": "LabProtocol",
            "name": "Dissection and collection of tissue from 10-week-old male mice",
            "description": "Surgical dissection and collection of cortex, pituitary, and liver tissues from 10-week-old male mice, performed by Jackson Laboratory surgical team (Bar Harbor, ME, USA). Tissues snap-frozen and shipped internationally to Australia via dry shipper using FedEx.",
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
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                    "text": "# SOP: Dissection and Collection of Tissue from 10-Week-Old Male Mice\n\n**Applies to:** 10-week-old male mice (C57BL/6J, DBA/2J, or other inbred strains)\n**Tissues collected:** Cortex, pituitary, liver\n**Performed by:** Surgical team, The Jackson Laboratory, Bar Harbor, ME, USA\n**Shipping:** Dry shipper via FedEx International Priority to Australia\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nTo obtain high-quality, snap-frozen tissue samples (cortex, pituitary, liver) from 10-week-old male mice for downstream molecular analysis (RNA extraction, DNA extraction, proteomics). Tissues are collected at The Jackson Laboratory and shipped internationally to Australia under cryogenic conditions to preserve nucleic acid and protein integrity.\n\n## Scope\n\nAll personnel involved in the planning, coordination, dissection, tissue collection, and shipment of mouse tissue samples between The Jackson Laboratory (USA) and the receiving laboratory (Australia).\n\n## Responsibilities\n\n* **Principal Investigator (receiving lab):** Define experimental design, strain requirements, tissue list, and sample labelling scheme. Coordinate import permits and institutional approvals.\n* **Jackson Laboratory surgical team:** Perform euthanasia, dissection, tissue collection, snap-freezing, and packaging for shipment.\n* **Shipping coordinator:** Arrange FedEx International Priority shipment, customs documentation, and dry shipper logistics.\n* **Receiving lab personnel:** Receive shipment, verify sample integrity, transfer to long-term storage, and log receipt.\n\n## Regulatory and Ethical Requirements\n\n* All animal procedures must be approved by The Jackson Laboratory IACUC (Institutional Animal Care and Use Committee) under an active protocol.\n* Receiving institution must hold a valid **import permit** for biological materials from the relevant national authority (e.g., Australian Department of Agriculture, Fisheries and Forestry \u2014 DAFF).\n* Ensure compliance with CITES regulations (not typically applicable for laboratory mice, but verify for specific strains).\n* **Biosecurity:** Frozen tissues from SPF (Specific Pathogen Free) mice. Health monitoring reports from Jackson Laboratory should accompany the shipment.\n\n---\n\n## Materials and Equipment\n\n### At Jackson Laboratory (dissection)\n\n* Certified CO\u2082 euthanasia station or injectable anaesthetic (as per IACUC protocol)\n* Cervical dislocation tools (secondary method of euthanasia)\n* Sterile surgical instruments: fine scissors, forceps (curved and straight), scalpel with #10 and #15 blades, micro-dissecting forceps, bone rongeurs (for skull)\n* Sterile surgical drapes and pads\n* Sterile PBS (phosphate-buffered saline), ice-cold\n* Liquid nitrogen in Dewar flask\n* Pre-labelled cryovials (1.5\u20132.0 mL, cryogenic-rated, screw-cap)\n* Cryogenic labels (resistant to LN\u2082 and \u221280 \u00b0C)\n* RNAlater (optional, as backup for tissues not immediately snap-frozen)\n* Absorbent pads and biohazard waste bags\n* PPE: lab coat, gloves, face shield (for LN\u2082 handling)\n\n### For shipping\n\n* **Dry shipper** (vapour-phase liquid nitrogen dewar, e.g., MVE SC 4/2V or Taylor-Wharton CX100)\n* Cryogenic storage canes or racks\n* Secondary containment bags (leak-proof, absorbent material)\n* FedEx International Priority shipping account\n* Customs documentation: commercial invoice, packing list, import permit, health certificate, AQIS declaration\n* Temperature data logger (optional but recommended)\n\n### At receiving laboratory (Australia)\n\n* \u221280 \u00b0C ultra-low freezer with allocated space\n* Cryogenic gloves and face shield\n* Sample inventory system / database\n\n---\n\n## Procedure\n\n### 1. Pre-Dissection Planning\n\n1. Confirm **mouse strain, sex (male), and age (10 weeks \u00b1 2 days)** with Jackson Laboratory.\n2. Provide Jackson Laboratory with:\n   * Number of animals required (biological replicates)\n   * Tissue list: **cortex, pituitary, liver**\n   * Sample labelling scheme (e.g., strain_tissue_replicate: C57_CTX_1, DBA_PIT_3)\n   * Any special handling instructions (e.g., morning collection to control circadian effects)\n3. Confirm **IACUC protocol** is active and covers the planned procedures.\n4. Obtain **Australian import permit** (DAFF) for frozen animal tissues. Allow 4\u20136 weeks lead time.\n5. Arrange **FedEx International Priority** account and confirm dry shipper availability and return logistics.\n\n### 2. Animal Preparation\n\n1. House mice under standard conditions (12 h light/dark cycle, ad libitum food and water, 20\u201324 \u00b0C) for at least 1 week prior to collection to minimise stress-related transcriptomic changes.\n2. Record for each animal:\n   * Strain and stock number\n   * Date of birth and age at collection\n   * Body weight\n   * Cage number and housing conditions\n   * Any health observations or abnormalities\n3. Fast animals for **4\u20136 hours** prior to collection if liver metabolomic consistency is required (optional \u2014 confirm with PI).\n\n### 3. Euthanasia\n\n1. Euthanise by **CO\u2082 inhalation** (gradual displacement, 30\u201370% chamber volume/min) followed by **cervical dislocation** as secondary confirmation, in accordance with AVMA Guidelines and IACUC protocol.\n2. Confirm death: absence of heartbeat, respiration, and pedal reflex.\n3. Record **time of death** \u2014 proceed to dissection immediately (within 5 min) to minimise RNA degradation.\n\n### 4. Tissue Dissection and Collection\n\nPerform all dissections on a clean, sterile surface. Use separate sterile instruments for each tissue to prevent cross-contamination. Work quickly \u2014 total dissection time per animal should be **<15 minutes**.\n\n#### 4a. Brain \u2014 Cortex\n\n1. Decapitate the mouse. Remove the skin overlying the skull.\n2. Using bone rongeurs or fine scissors, carefully open the skull along the sagittal suture from the foramen magnum to the olfactory bulbs.\n3. Gently lift and peel back the skull plates to expose the brain.\n4. Using curved forceps, carefully lift the brain from the cranial cavity, severing cranial nerves and the spinal cord at the brainstem.\n5. Place the brain dorsal-side up on a cold surface (glass plate on ice or cold PBS-dampened filter paper).\n6. Using a scalpel or razor blade, remove the **cerebral cortex** by making a coronal cut posterior to the olfactory bulbs and peeling the cortical hemispheres away from the underlying structures (hippocampus, striatum, thalamus).\n7. Transfer cortex tissue immediately to a **pre-labelled cryovial**.\n8. **Snap-freeze** by plunging the cryovial into liquid nitrogen. Hold submerged for \u226530 seconds.\n\n#### 4b. Pituitary\n\n1. After removing the brain, examine the base of the skull (sella turcica).\n2. The **pituitary gland** is a small (~1 mm), pinkish structure sitting in the sella turcica.\n3. Using fine-tipped forceps, gently tease the pituitary free from the surrounding membrane and bone.\n4. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen immediately.\n5. **Note:** The pituitary is very small \u2014 take care not to lose or fragment it. If it fragments, collect all pieces.\n\n#### 4c. Liver\n\n1. Open the abdominal cavity with a midline ventral incision from pelvis to sternum.\n2. Retract the intestines to expose the liver.\n3. Identify the **left lateral lobe** (largest, most accessible lobe).\n4. Excise a **~50\u2013100 mg piece** (approximately 5 \u00d7 5 \u00d7 3 mm) from the left lateral lobe using a clean scalpel or razor blade.\n5. Briefly blot on sterile filter paper to remove excess blood.\n6. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen.\n7. If the entire liver is required, excise all lobes, record total liver weight, and snap-freeze in separate vials.\n\n### 5. Post-Dissection\n\n1. Transfer all snap-frozen cryovials from liquid nitrogen to a **\u221280 \u00b0C freezer** within 1 hour of collection.\n2. Dispose of carcasses and biological waste according to Jackson Laboratory institutional procedures.\n3. Clean and decontaminate all instruments and work surfaces.\n4. Complete the **dissection log** for each animal:\n\n   | Field | Record |\n   |---|---|\n   | Animal ID | Strain, stock #, ear tag/toe clip |\n   | Date and time of euthanasia | |\n   | Body weight (g) | |\n   | Tissues collected | Cortex, pituitary, liver |\n   | Tissue appearance | Normal / abnormal (describe) |\n   | Cryovial labels | List all vial IDs |\n   | Operator | Name and signature |\n   | Notes | Any deviations or observations |\n\n### 6. Packaging for International Shipment\n\n1. **Charge the dry shipper** with liquid nitrogen at least 24 hours before packing. Drain excess liquid \u2014 the dry shipper must contain only **vapour-phase nitrogen** (no free liquid) for air transport compliance (IATA).\n2. Verify the dry shipper holds temperature below **\u2212150 \u00b0C** and has a rated hold time sufficient for transit (typically 10\u201314 days).\n3. Place cryovials in **cryogenic canes or racks** and insert into the dry shipper.\n4. Seal the dry shipper and place in the **outer shipping container** (insulated box or hard case).\n5. Include **absorbent material** inside the secondary containment bag (regulatory requirement for biological specimens).\n6. Attach a **temperature data logger** (optional but recommended) to monitor transit conditions.\n7. Prepare shipping documentation:\n   * **Commercial invoice** (contents: frozen mouse tissue samples, no commercial value / research use only)\n   * **Packing list** (number of vials, tissue types, strain, animal IDs)\n   * **Australian import permit** (DAFF-issued)\n   * **Health monitoring report** from Jackson Laboratory (SPF status)\n   * **Dangerous goods declaration** \u2014 Class 9 UN3373 Biological Substance Category B (if required by carrier) or exempt if shipped as non-infectious, non-hazardous research specimens in a dry shipper\n   * **IATA shipper's declaration** for magnetized material (dry shipper contains no free liquid but is classified as a cryogenic vessel)\n\n### 7. Shipment\n\n1. Schedule **FedEx International Priority** pickup from Jackson Laboratory (Bar Harbor, ME, USA).\n2. Ship **Monday\u2013Wednesday** to avoid weekend delays at customs.\n3. Estimated transit time: **3\u20135 business days** (Bar Harbor \u2192 Australian destination).\n4. Provide the receiving lab with the **tracking number** and expected arrival date.\n5. Alert the receiving lab to arrange customs clearance and immediate collection upon arrival.\n\n### 8. Receipt at Australian Laboratory\n\n1. **Collect the shipment immediately** upon arrival \u2014 do not allow it to sit at the depot.\n2. Open the dry shipper and verify:\n   * All cryovials are present and match the packing list\n   * Vials are still frozen (frost visible on vials)\n   * No signs of thawing (condensation, liquid in vials)\n   * Temperature data logger reading (if included) \u2014 confirm temperature remained below \u2212150 \u00b0C throughout transit\n3. Transfer all cryovials immediately to a **\u221280 \u00b0C freezer**.\n4. Log receipt in the laboratory sample inventory:\n\n   | Field | Record |\n   |---|---|\n   | Date received | |\n   | Shipment tracking # | |\n   | Dry shipper condition | Frost present / temperature OK |\n   | Number of vials received | |\n   | Vials match packing list? | Yes / No (note discrepancies) |\n   | Storage location | Freezer ID, shelf, box, position |\n   | Received by | Name and signature |\n\n5. Return the **dry shipper** to Jackson Laboratory per agreed logistics (typically FedEx return shipment).\n\n---\n\n## Quality Control\n\n* **RNA integrity:** Downstream RNA extraction should target RIN/RQN \u2265 7.0 for all tissues. Pituitary may yield lower due to small size \u2014 RQN \u2265 5.0 acceptable.\n* **Dissection time:** Total time from euthanasia to snap-freeze should be **<15 minutes per animal**. Record actual times.\n* **Freeze-thaw:** Tissues must not undergo any freeze-thaw cycles before processing. Monitor freezer temperature logs.\n\n## Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Pituitary fragmented or lost | Record in log; may need to pool from multiple animals or exclude |\n| Tissue partially thawed on arrival | Record in log; process immediately for extraction; note in downstream QC |\n| Customs delay >48 h | Contact FedEx and customs broker; verify dry shipper hold time is sufficient |\n| Dry shipper running low | If hold time is marginal, arrange emergency transfer to local \u221280 \u00b0C |\n| Import permit expired | Do not ship; renew permit before proceeding (allow 4\u20136 weeks) |\n| Animal health abnormality | Record observation; PI to decide whether to include or exclude the animal |\n\n## Waste Disposal\n\n* Mouse carcasses: dispose per Jackson Laboratory IACUC-approved procedures (incineration or licensed biological waste contractor).\n* Sharps (scalpel blades, needles): sharps container.\n* Liquid nitrogen: allow to evaporate in well-ventilated area. Do not pour down drains.\n\n## Records to Maintain\n\n* Approved IACUC protocol number\n* Australian import permit (DAFF) \u2014 copy\n* Dissection logs for each animal\n* Shipping documentation (commercial invoice, packing list, tracking number)\n* Temperature logger data (if used)\n* Sample receipt log\n* Freezer storage map / inventory database entry\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-40",
            "@type": "LabProtocol",
            "name": "Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit",
            "description": "Simultaneous purification of genomic DNA and total RNA from the same tissue sample (\u226430 mg) using selective AllPrep DNA spin columns and RNeasy silica membrane technology. Includes on-column DNase treatment for RNA.",
            "url": {
                "@id": "https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/multianalyte-and-virus/allprep-dna-rna-mini-kit"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "# SOP: Simultaneous DNA and RNA Extraction from Animal Tissues using QIAGEN AllPrep DNA/RNA Mini Kit\n\n**Applies to:** Fresh or frozen animal tissues \u226430 mg per spin column\n**Kit:** QIAGEN AllPrep DNA/RNA Mini Kit (Cat. No. 80204)\n**Location:** __________\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nSimultaneous purification of genomic DNA and total RNA from the same tissue sample using the AllPrep DNA spin column (selective DNA binding in high-salt buffer) and the RNeasy spin column (silica membrane RNA binding). This enables paired DNA/RNA analysis from a single biopsy or tissue aliquot.\n\n## Scope\n\nAll personnel performing dual DNA/RNA extraction from animal tissues in this laboratory.\n\n## Responsibilities\n\n* **Operators:** Follow this SOP, record batch details, and maintain RNase-free practice.\n* **Supervisors:** Ensure training, PPE compliance, and kit maintenance.\n\n## Safety and RNase-Free Practice\n\n* Wear lab coat, gloves, and eye protection.\n* Work in a clean area. Treat benches and tools with RNase decontamination solution.\n* \u03b2-mercaptoethanol is toxic and has a strong odor. Handle in a fume hood.\n* Use RNase-free tubes, tips, and reagents only. Change gloves frequently.\n* Buffer RLT Plus contains guanidine isothiocyanate \u2014 harmful if swallowed or in contact with skin. Do not mix with bleach.\n\n## References\n\n* QIAGEN AllPrep DNA/RNA Mini Handbook, November 2020.\n* NASA GeneLab SOP GL-SOP-3.1 v1.0.\n\n---\n\n## Materials and Equipment\n\n**Provided in kit (Cat. No. 80204, 50 preps)**\n\n* Buffer RLT Plus\n* Buffer RW1\n* Buffer RPE (concentrate \u2014 add ethanol before first use)\n* Buffer AW1\n* Buffer AW2\n* AllPrep DNA Mini Spin Columns\n* RNeasy Mini Spin Columns\n* Collection Tubes (1.5 mL and 2 mL)\n* RNase-free Water\n\n**Not supplied**\n\n* \u03b2-mercaptoethanol (\u03b2-ME)\n* 96\u2013100% ethanol\n* 70% ethanol (freshly prepared)\n* RNase-free DNase Set (Cat. No. 79254) \u2014 for on-column DNase treatment\n* RNase/DNase-free 1.5 mL tubes\n* Microcentrifuge capable of \u226512,000 \u00d7 g\n* Homogenization tools: rotor-stator homogenizer, or TissueLyser with stainless steel beads (3\u20137 mm), or RNase-free pestle, or mortar and pestle with liquid nitrogen\n* Optional: RNase-free syringe with 20-gauge needle\n\n---\n\n## Preparations (before each extraction session)\n\n1. **Prepare Buffer RPE** (first use only)\n   * Add **4 volumes of 96\u2013100% ethanol** to the concentrate. Mark the bottle to indicate ethanol has been added.\n\n2. **Prepare fresh Lysis Buffer**\n   * Add **10 \u00b5L \u03b2-mercaptoethanol per 1 mL Buffer RLT Plus**. Mix well. Prepare only what you need; discard unused buffer.\n\n3. **Prepare DNase Mix** (per sample)\n   * Dissolve lyophilized DNase I (1500 Kunitz units) in **550 \u00b5L** RNase-free water. Mix gently. Aliquot and store at \u221220 \u00b0C (stable up to 9 months; thawed aliquots stable at 2\u20138 \u00b0C for 6 weeks).\n   * Per sample: **10 \u00b5L DNase I stock + 70 \u00b5L Buffer RDD**. Mix by gentle inversion \u2014 **do not vortex**.\n\n4. **Pre-chill microcentrifuge** to 4 \u00b0C if required for lysate clarification.\n\n5. **Heat 2 mL RNase/DNase-free water** to 70 \u00b0C for DNA elution (optional, improves yield).\n\n6. **Input tissue mass**\n\n   | Tissue type | Maximum input |\n   |---|---|\n   | Standard soft tissue | \u226430 mg |\n   | Muscle, heart, skin | \u226415 mg (LN\u2082-preserved) or \u226410 mg (RNAlater) |\n   | Liver | \u226420 mg |\n\n---\n\n## Procedure\n\n### 1. Tissue Disruption and Homogenization\n\n1. Weigh tissue (\u226430 mg). Place in a pre-chilled RNase-free tube on ice.\n2. Add **600 \u00b5L Buffer RLT Plus** (with \u03b2-ME).\n3. Homogenize immediately using one of the following:\n\n   **Option A \u2014 Rotor-stator:**\n   Homogenize for **30\u201345 s** until no visible tissue pieces remain.\n\n   **Option B \u2014 TissueLyser / bead mill:**\n   Add a single **5 mm stainless steel bead**. Run at **25 Hz for 2 \u00d7 2 min** (rotate adapter halfway).\n\n   **Option C \u2014 Mortar & pestle with liquid nitrogen:**\n   Grind tissue to a fine powder under LN\u2082. Transfer powder to a tube, let LN\u2082 evaporate, then add Buffer RLT Plus and vortex.\n\n   **Option D \u2014 Needle and syringe:**\n   Pass lysate through a **20-gauge needle** at least **5\u201310 times** until homogeneous.\n\n4. Centrifuge the lysate at **full speed (\u226512,000 \u00d7 g) for 3 min**, RT.\n5. Carefully transfer the supernatant to a new tube. **Do not disturb the pellet.**\n\n### 2. Bind Genomic DNA\n\n1. Transfer the cleared lysate onto an **AllPrep DNA Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 30 s**, RT.\n3. **Set aside the DNA column** at 4 \u00b0C (or RT for up to 1 h) \u2014 proceed to RNA purification first.\n4. **Save the flow-through** for RNA isolation.\n\n### 3. Adjust RNA Binding Conditions\n\n1. Add **1 volume of freshly prepared 70% ethanol** to the flow-through (e.g. 600 \u00b5L ethanol to 600 \u00b5L flow-through). For **liver tissue**, use **50% ethanol** instead.\n2. Mix well by pipetting. **Do not centrifuge.** A precipitate may form \u2014 this is normal; proceed immediately.\n\n### 4. Bind and Wash RNA\n\n1. Transfer up to **700 \u00b5L** of the sample onto an **RNeasy Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Repeat until all sample has passed through the column.\n4. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 5. On-Column DNase Treatment\n\n1. Pipette **80 \u00b5L DNase Mix** (10 \u00b5L DNase I + 70 \u00b5L Buffer RDD) directly onto the membrane.\n2. Incubate at **RT for 15\u201330 min** (30 min recommended for maximum removal).\n3. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 6. Wash and Dry RNA Column\n\n1. **Replace the collection tube** with a new 2 mL tube.\n2. Add **500 \u00b5L Buffer RPE** (ethanol added). Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add another **500 \u00b5L Buffer RPE**. Centrifuge at **\u22658,000 \u00d7 g for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 7. Elute RNA\n\n1. Place the RNeasy column in a new **1.5 mL collection tube**.\n2. Add **30\u201350 \u00b5L RNase-free water** directly onto the membrane.\n3. Incubate **1\u20135 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** RNase-free water for maximum yield. Pool eluates if the second elution is \u226530% of the first.\n6. Place RNA on ice immediately.\n\n### 8. Wash Genomic DNA Column\n\n1. Retrieve the AllPrep DNA column from step 2.\n2. Add **500 \u00b5L Buffer AW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add **500 \u00b5L Buffer AW2**. Centrifuge at **full speed (\u226514,000 \u00d7 g) for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 9. Elute Genomic DNA\n\n1. Place the AllPrep DNA column in a new **1.5 mL collection tube**.\n2. Add **50\u2013100 \u00b5L RNase/DNase-free water** (pre-warmed to 70 \u00b0C for improved yield) directly onto the membrane.\n3. Incubate **2 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** for maximum yield.\n\n### 10. Quantification and Quality Assessment\n\n**RNA:**\n* Measure concentration by Qubit RNA assay or NanoDrop A260.\n* Record A260/280 (expect \u22651.8) and A260/230 (expect \u22651.5) ratios.\n* Assess integrity by Fragment Analyzer, Bioanalyzer, or TapeStation (target RQN/RIN \u22657 for most applications).\n\n**DNA:**\n* Measure concentration by Qubit dsDNA assay or NanoDrop A260.\n* Record A260/280 (expect ~1.8) and A260/230 ratios.\n* Assess fragment size by TapeStation Genomic DNA assay or agarose gel if required.\n\n### 11. Storage\n\n* **RNA:** Keep on ice for immediate use. Store at **\u221280 \u00b0C** for long term.\n* **DNA:** Store at **\u221220 \u00b0C** (short term) or **\u221280 \u00b0C** (long term). Avoid repeated freeze-thaw cycles.\n\n---\n\n## Special Considerations for Muscle, Heart, and Skin Tissues\n\nThese tissues are dense and fibrous, leading to potential column clogging and lower yields.\n\n* Start with **\u226415 mg** (LN\u2082-preserved) or **\u226410 mg** (RNAlater-preserved).\n* **Pre-warm** Buffers RW1 and RPE to **37 \u00b0C** for 30 min before use.\n* Centrifuge lysate **twice** at full speed to minimize cell debris.\n* Load a maximum of **400 \u00b5L** per column pass.\n* If the column clogs: incubate wash solution on column for **5 min at 37 \u00b0C** before centrifuging.\n* Elute with **pre-warmed (37 \u00b0C) water** in two passes (50 \u00b5L + 30 \u00b5L); combine if second elution is \u226530% of first.\n* If yields remain low: process **two 15 mg aliquots separately** and combine eluates.\n\n---\n\n## Acceptance and Rejection Criteria\n\n* Elution volume and expected yield range documented per tissue type.\n* No visible carryover of debris in eluate.\n* Proceed if spectrophotometric and integrity checks meet project-specific thresholds.\n\n## Expected Yields\n\n| Tissue type | DNA yield | RNA yield |\n|---|---|---|\n| Liver (20 mg) | 10\u201330 \u00b5g | 20\u201360 \u00b5g |\n| Brain (20 mg) | 5\u201315 \u00b5g | 5\u201320 \u00b5g |\n| Kidney (20 mg) | 5\u201315 \u00b5g | 10\u201340 \u00b5g |\n| Heart/Muscle (15 mg) | 2\u201310 \u00b5g | 1\u201310 \u00b5g |\n| Spleen (20 mg) | 15\u201340 \u00b5g | 15\u201360 \u00b5g |\n\n## Troubleshooting\n\n| Problem | Possible cause | Solution |\n|---|---|---|\n| Low RNA yield | Incomplete lysis | Increase homogenization time; ensure \u03b2-ME was added to RLT Plus |\n| Low RNA yield | Column overloaded | Reduce input tissue mass |\n| Low DNA yield | Insufficient lysis | Ensure thorough mechanical disruption before loading AllPrep DNA column |\n| Low DNA yield | DNA column dried out | Do not leave DNA column at RT for >1 h |\n| Genomic DNA in RNA | DNase treatment insufficient | Extend incubation to 30 min; verify DNase I activity |\n| RNA in DNA | Carryover of flow-through | Avoid disturbing interface when transferring lysate |\n| Column clogging | Tissue debris | Pre-clear lysate by additional centrifugation; reduce input mass |\n| Low A260/230 | Guanidine salt carryover | Add an extra RPE/AW2 wash step |\n\n## Waste Disposal\n\n* Collect \u03b2-mercaptoethanol and guanidine-containing wastes according to institutional chemical waste procedures.\n* Dispose of biological materials under local biosafety rules.\n* Spin columns with bound nucleic acids are non-hazardous after elution.\n\n## Records to Maintain\n\n* Date, operator, sample ID, tissue type and mass, homogenization method, reagent lot numbers, elution volume(s), DNA and RNA concentrations, purity ratios, integrity assessment, and storage location.\n* Deviations and corrective actions.\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-37",
            "@type": "LabProtocol",
            "name": "PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)",
            "description": "Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library preparation from total RNA. Uses template-switching reverse transcription followed by PCR amplification with barcoded primers. Compatible with MinION, GridION and PromethION flow cells (R9.4.1). Superseded by SQK-PCS114 in late 2023.",
            "url": {
                "@id": "https://community.nanoporetech.com/docs/prepare/library_prep_protocols/pcr-cdna-barcoding-kit-sqk-pcb111-24"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "## Overview & Kit Contents\n\nThis protocol is based on the Oxford Nanopore Technologies **PCR-cDNA Barcoding Kit (SQK-PCB111.24)**, suitable for generating full-length barcoded cDNA sequencing libraries from total RNA.\n\n### Kit components\n- VNP primer, SSP primer (strand-switching primer)\n- Barcoded PCR primers (BC01\u2013BC24)\n- Rapid Adapter (RAP)\n- Elution Buffer (EB)\n\n### Required reagents (user-supplied)\n- Lambda Exonuclease\n- NEBNext Quick Ligation Reaction Buffer\n- T4 DNA Ligase 2M U/ml\n- 10 mM dNTP solution\n- LongAmp Hot Start Taq 2X Master Mix\n- Maxima H Minus Reverse Transcriptase (200 U/ul) with 5x RT Buffer\n- RNaseOUT, 40 U/ul\n- USER (Uracil-Specific Excision Reagent) Enzyme\n- Exonuclease I\n- AMPure XP beads\n\n### Required equipment\n- Thermal cycler, magnetic rack, Qubit fluorometer, vortex mixer, microcentrifuge"
                },
                {
                    "@type": "HowToStep",
                    "position": 2,
                    "text": "### Input RNA requirements\n\n- **Input**: 1\u201350 ng poly(A)+ mRNA or 50\u2013100 ng total RNA (RIN >= 7 recommended)\n- RNA should be DNase-treated if genomic DNA contamination is suspected\n- Prepare RNA in 7.5 uL nuclease-free water\n- Add 1 uL VNP primer (2 uM) and 1 uL 10 mM dNTPs\n- Incubate at **65 C for 5 min**, then snap-cool on ice for 1 min"
                },
                {
                    "@type": "HowToStep",
                    "position": 3,
                    "text": "### Reverse transcription and strand switching (~90 min)\n\n1. Prepare RT mix on ice (per reaction):\n   - 4 uL 5x RT Buffer\n   - 1 uL RNaseOUT (40 U/uL)\n   - 1 uL nuclease-free water\n   - 2 uL Strand-Switching Primer (SSP, 10 uM)\n   - 1 uL Maxima H Minus RT (200 U/uL)\n2. Add 10 uL RT mix to the 9.5 uL RNA/primer/dNTP mix (total 19.5 uL)\n3. Incubate: **42 C for 90 min** (RT + template switching), **85 C for 5 min** (inactivation), hold 4 C\n4. Add 1 uL RNase H, incubate **37 C for 10 min**\n5. Briefly centrifuge and place on ice"
                },
                {
                    "@type": "HowToStep",
                    "position": 4,
                    "text": "### PCR amplification with barcoded primers (~30 min)\n\n1. Prepare PCR reaction (50 uL total):\n   - 20 uL cDNA from RT step\n   - 25 uL LongAmp Taq 2X Master Mix\n   - 1.5 uL Barcode Primer (BC*XX*, 10 uM)\n   - 3.5 uL nuclease-free water\n2. Cycling conditions:\n   - **95 C, 3 min** (initial denaturation)\n   - **18 cycles**: 95 C 15 s / 62 C 15 s / 65 C 5 min\n   - **65 C, 6 min** (final extension)\n   - Hold at 4 C\n\n**Note**: 18 cycles is suitable for 50-100 ng total RNA input. Reduce to 14-15 cycles for higher inputs."
                },
                {
                    "@type": "HowToStep",
                    "position": 5,
                    "text": "### Bead clean-up and quantification\n\n1. Add 40 uL AMPure XP beads (0.8x ratio) to 50 uL PCR product\n2. Incubate at RT for 5 min\n3. Place on magnetic rack, wait for pellet to form\n4. Wash 2x with 200 uL freshly prepared 80% ethanol\n5. Air-dry pellet for ~30 s (do not over-dry)\n6. Elute in 12 uL EB\n7. Quantify with Qubit dsDNA HS assay\n8. Expected yield: 50-500 ng depending on input RNA quality and quantity"
                },
                {
                    "@type": "HowToStep",
                    "position": 6,
                    "text": "### Pool barcoded libraries and attach Rapid Adapter\n\n1. Pool equimolar amounts of barcoded cDNA libraries (target: 100-200 fmol total)\n2. Adjust volume to 11 uL with EB\n3. Add 1 uL Rapid Adapter (RAP)\n4. Mix gently by flicking, spin down briefly\n5. Incubate at **RT for 5 min**\n6. Library is ready for loading -- **do not vortex** after adapter attachment"
                },
                {
                    "@type": "HowToStep",
                    "position": 7,
                    "text": "### Flow cell priming and loading\n\n1. Prepare priming mix: 30 uL FCT + 1170 uL FCF\n2. Load 800 uL priming mix via priming port, wait 5 min\n3. Prepare loading mix: 12 uL adapted library + 37.5 uL SB + 25.5 uL LB\n4. Load remaining priming mix, then load 75 uL library via SpotON port\n5. Start sequencing in MinKNOW (Kit: SQK-PCB111.24, enable barcode demultiplexing)"
                },
                {
                    "@type": "HowToStep",
                    "position": 8,
                    "text": "## References\n\n- Oxford Nanopore Technologies, **\"PCR-cDNA Barcoding Kit (SQK-PCB111.24)\"**.\n- Earlier version: SQK-PCS111. Kit superseded by SQK-PCS114/SQK-PCB114 (V14 chemistry, R10.4.1 flow cells) in late 2023."
                }
            ]
        },
        {
            "@id": "#protocol-44",
            "@type": "LabProtocol",
            "name": "Fragment Analyzer \u2013 SS NGS Fragment 1-6000 bp",
            "description": "Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-473 kit. Suitable for QC of NGS libraries, PCR products, and size-selected DNA fragments in the 1-6000 bp range.",
            "url": {
                "@id": "https://www.agilent.com/store/en_US/Prod-DNF-473-0500/DNF-473-0500"
            },
            "version": "1.0",
            "category": "measurement",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "**Method:** `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds`\n\n**Instrument:** Agilent/Advanced Analytical Fragment Analyzer (33 cm capillary array)\n\n**Analysis Mode:** NGS\n\n---\n\n## 1. Document Control\n\n* **SOP ID:** FA-NGS-1-6000bp-33cm\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Prepared by:** __________\n* **Approved by:** __________\n* **Review cycle:** 12 months\n\n## 2. Purpose\n\nTo define the procedure for running standard-sensitivity NGS fragment analysis on the Fragment Analyzer using the **DNF-473-33** method. This method resolves DNA fragments in the **1\u20136,000 bp** range with standard sensitivity, suitable for:\n\n* QC of NGS libraries (amplicon, PCR-cDNA, shotgun)\n* Assessment of size selection efficiency (e.g. AMPure bead selection)\n* Verification of library size distribution prior to sequencing\n* Quantification of library concentration\n\n## 3. Scope\n\nApplies to all operators performing NGS library QC or fragment analysis in the 1\u20136,000 bp range using the DNF-473 kit on a Fragment Analyzer.\n\n## 4. Responsibilities\n\n* **Operator:** Execute this SOP, record run metadata, verify QC, and report issues.\n* **Lab lead:** Ensure training, instrument maintenance, and SOP review.\n\n## 5. Safety\n\n* Wear lab coat, safety glasses, and disposable gloves.\n* Handle gels, buffers, and plates per SDS.\n* Avoid contact with high voltage areas during operation. Only open instrument when software indicates safe.\n* Dispose of consumables as per local regulations.\n\n## 6. References\n\n* Fragment Analyzer user manual.\n* DNF-473 SS NGS Fragment 1-6000bp kit instructions.\n* Local laboratory policies for data retention and equipment maintenance.\n\n## 7. Materials and Equipment\n\n**Instrument and configuration**\n\n* Fragment Analyzer with **33 cm** capillary array.\n* Array serial and usage count recorded before each run.\n\n**Reagents and consumables**\n\n* SS NGS Fragment 1-6000bp gel cartridge compatible with `DNF-473-33`.\n* Buffer reservoirs and rinse solution as per kit.\n* 96-well plate(s) and plate seals or caps.\n* DNA ladder (supplied with kit or compatible sizing standard).\n* Nuclease-free water and low-retention tips.\n\n**Software**\n\n* ProSize 3.0 or later for data analysis and PDF report generation.\n\n## 8. Sample Requirements\n\n* DNA libraries in TE, EB, or nuclease-free water.\n* Recommended input: **0.2\u201350 ng/\u00b5L** (standard sensitivity range).\n* Minimum volume per well: as per plate format (typically 2 \u00b5L sample + diluent).\n* Avoid EDTA >5 mM, high salt, or detergents that interfere with electrokinetic injection.\n* For very low concentration libraries (<1 ng/\u00b5L, e.g. post-bead-selection), results may show concentration but no resolved peaks \u2014 this is expected and the TIM value is still informative.\n\n## 9. Pre-Run Checks\n\n1. Inspect capillary array for leaks, kinks, or salt crystallisation. Record **array serial** and **usage count**.\n2. Confirm instrument details in software:\n   * **Device Serial:** record\n   * **FA Version:** record\n3. Verify consumables:\n   * Correct **gel** loaded and **Gel Selection: Gel 1**.\n   * Fresh buffer and rinse trays in correct positions.\n4. Equilibrate reagents to room temperature if required by kit.\n5. Prepare plate map with samples, ladder, controls, and blanks.\n\n## 10. Plate Preparation\n\n1. Thaw and mix reagents gently. Spin down briefly.\n2. Aliquot ladder into designated well (typically position 12 of each row).\n3. Aliquot samples into designated wells per plate map.\n4. Include at least one blank (nuclease-free water) for baseline reference.\n5. Seal the plate. Briefly centrifuge to remove bubbles. Keep sealed until loading.\n\n## 11. Instrument Setup (Software)\n\nSet parameters as follows:\n\n| Parameter | Setting |\n|---|---|\n| Method Name | `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds` |\n| Analysis Mode | `NGS` |\n| Gel Prime | `No` |\n| Full Conditioning | `Yes` (start of day) |\n| Gel Selection | `Gel 1` |\n| Perform Prerun | `5.0 kV` for `30 s` |\n| Rinse | Tray `3`, Row `A`, `# Dips: 2` |\n| Marker 1 | `No` |\n| Sample Injection | `5.0 kV` for `4 s` |\n| Separation | `8.0 kV` for `40.0 min` |\n| # of Capillaries | Per array configuration |\n\n**Note:** The DNF-473 method uses **shorter separation time (40 min)** and **higher separation voltage (8 kV)** compared to the HS Large Fragment method (DNF-464: 60 min, 5 kV), reflecting the smaller target size range.\n\n## 12. Run Procedure\n\n1. Load the sealed sample plate into the instrument.\n2. Confirm tray positions and liquid levels.\n3. In software, confirm the method parameters match Section 11.\n4. Start the run. Observe for the first 2\u20133 minutes for abnormal current, leaks, or errors.\n5. Allow the **40 min** separation to complete.\n\n## 13. Data Analysis (ProSize)\n\n1. Open the completed run in ProSize 3.0.\n2. Verify ladder sizing is correct (expected peaks at kit-specified positions).\n3. For each sample, review:\n   * **Electropherogram** \u2014 peak shape, baseline, and resolution\n   * **Total Concentration (ng/\u00b5L)** \u2014 integrated from all non-marker peaks\n   * **TIM (nmol/L)** \u2014 total integrated molarity\n   * **Main peak size (bp)** \u2014 dominant fragment size\n   * **Number of peaks** \u2014 indicator of library complexity/purity\n4. Export PDF report containing per-sample peak tables, electropherograms, and summary metrics.\n\n## 14. Interpretation Guide\n\n| Observation | Interpretation | Action |\n|---|---|---|\n| Single clean peak at expected size | Library prep and/or size selection successful | Proceed to pooling/sequencing |\n| Broad peak or multiple peaks | Heterogeneous library or incomplete size selection | Consider re-selection or accept depending on application |\n| No peaks but measurable concentration | Library below detection threshold for sizing | Verify by Qubit; may still be usable if concentration is sufficient |\n| Adapter dimer peak (~100-150 bp) | Incomplete cleanup | Re-clean with AMPure beads at appropriate ratio |\n| No signal | Failed library prep, sample degraded, or loading error | Troubleshoot: check sample, re-run, or re-prep |\n\n### Typical Results for ONT PCR-cDNA Libraries\n\n| Library state | Expected concentration | Expected main peak |\n|---|---|---|\n| Pre-size-selection | 4\u201310 ng/\u00b5L | 1,000\u20132,000 bp (broad) |\n| Post-bead-selection (1.5 kb) | 0.5\u20132 ng/\u00b5L | ~1,480 bp (single peak) |\n| Post-bead-selection (4 kb) | 0.5\u20132 ng/\u00b5L | ~3,500\u20134,500 bp (single peak) |\n\n## 15. Quality Control and Acceptance\n\n* Ladder wells exhibit expected profile and sizing.\n* Baseline is stable with distinct peaks where applicable.\n* Migration time consistent across capillaries.\n* If any criterion fails, document, recondition, and repeat controls before analysing unknowns.\n\n## 16. Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Weak or absent signal | Verify injection settings, sample concentration, plate seal integrity, capillary tips in wells |\n| High noise or drifting baseline | Re-condition array, check buffer freshness and tray cleanliness |\n| Inconsistent sizing | Confirm ladder integrity, plate map accuracy, and temperature stability |\n| Frequent current faults | Inspect for bubbles, low liquid levels, or salt contamination; replace consumables |\n\n## 17. Maintenance\n\n* Perform **Full Conditioning** at the start of the day or as required by kit.\n* Replace gels and buffers per shelf-life and use counts.\n* Track **Array Usage Count** at each run and follow vendor guidance for replacement.\n* Clean trays weekly or after spills. Inspect electrodes and stage area.\n\n## 18. Records to Retain\n\nFor each run record:\n\n* Date, time, and operator\n* Device serial and FA software version\n* Array serial and usage count\n* Method name and key parameters\n* Plate map and sample IDs\n* File path to raw data and PDF reports\n* QC outcomes and any deviations\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-35",
            "@type": "LabProtocol",
            "name": "Sequencing Submission",
            "description": "Submit samples for sequencing at the genomics facility. Links kevlab samples to QC app runs after sequencing is complete.",
            "version": "1.0",
            "category": "sequencing"
        },
        {
            "@id": "#measurement-344",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1493",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-345",
            "@type": "PropertyValue",
            "name": "count",
            "value": "3",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-342",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "5.6008",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-343",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "6.1563",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#measurement-348",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1493",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-349",
            "@type": "PropertyValue",
            "name": "count",
            "value": "4",
            "unitCode": "UO:0000189",
            "unitText": "count"
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