ONT Isoform Functional Annotation (superseded)

Barcode 23 functional annotation

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Type

CWL

Status

succeeded

Engine

cwltool

Duration

1.3 h

Source Data

Study	Strain-specific cortex gene expression and isoform usage
Sample prep	PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) 2023-05-09
Sequencing	Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) 2023-05-12
Run Data	Run #63 (6 samples)
Samples	C57_rep1_bc10 C57_rep2_bc11 C57_rep3_bc12 DBA_rep1_bc13 DBA_rep2_bc14 DBA_rep3_bc15

Pipeline

ONT Long-Read Transcriptomics (IsoQuant)

Run #45

succeeded

ONT Isoform Functional Annotation (superseded)

Run #52 (this run)

succeeded 1 sources

Combined From

#45 — ONT Long-Read Transcriptomics (IsoQuant) succeeded

Sample Provenance

Process Chain

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

8 samples produced

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

24 samples produced

Simultaneous extraction of DNA and RNA from C57 and DBA cortex tissue using Qiagen AllPrep (2016-11-17) Labbook

Protocol: Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit

16 samples produced

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24) (2023-03-13) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection) (2023-03-14) Labbook

24 measurements across 6 samples

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) (2023-05-09) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) (2023-05-12) Labbook

QC Measurements

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection)

2023-03-14 Labbook

Sample	bp	count	ng/µL	nmol/L
C57_rep1_bc19	1493	3	5.6008	6.1563
C57_rep2_bc20	1493	4	6.2061	7.4416
C57_rep3_bc21	1485	3	5.7241	6.8672
DBA_rep1_bc22	1500	2	4.2355	4.4695
DBA_rep2_bc23	1485	2	4.0452	4.2648
DBA_rep3_bc24	1485	2	4.3327	4.7107

Workflow

ONT Isoform Functional Annotation

#cwl

Software Tools

Tool	Version	URL
cwltool	-	https://github.com/common-workflow-language/cwltool
eldudy007_isoseq:1.0.sif	-	-

Results Summary

Unique Reads

378,555

from upstream transcriptomics

rRNA Reads

274 (0.1%)

mRNA Reads

378,281

used for CPM calculation

Transcripts Annotated

10,544

Pfam Hit Rate

94.4%

SwissProt Hit Rate

95.2%

High-Confidence

8,008 (75.9%)

Tier 1–2

NMD Candidates

283 (2.7%)

Evidence Tier Distribution

Novel Isoform Discovery

Total Isoforms

10,544

Reference

9,685

Novel (IsoQuant)

859

855 with ≥3 reads, 4 low-support

Novel %

8.1%

DV Genes: Novel-Created

238

Variation only from novel isoforms

DV Genes: Novel-Contributed

Novel isoforms add to existing ref variation

DV Genes: Reference-Only

564

Variation from reference annotation alone

Long-read sequencing discovered 859 novel isoforms (8.1%). Of 866 domain variant genes, 238 (27%) show domain architecture variation only because of novel isoforms not present in the reference annotation. In 7 DV genes, only novel isoforms produced detectable ORFs.

Isoform Read Support (CPM from upstream transcriptomics)

Novel Expression Rate

100.0%

859 / 859 (855 with ≥3 reads)

Reference Expression Rate

94.8%

9,180 / 9,685

Novel Median CPM

15.9

median 6 reads (mean 11.1, range 0–272)

Ref Median CPM

13.2

Novel (n=859)

Reference (n=9,685)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual isoform CPM values are overlaid as jittered points. Log₁₀ scale; dashed line marks CPM = 1 expression threshold. Per-model CPM from OUT.transcript_model_counts.tsv

Novel Isoform Functional Impact

Domain Altered

11.5% of novel

NMD Candidates

7.1% of novel

ORF Disrupted

100

11.6% of novel

Conserved

573

66.7% — same protein

820 of 859 novel isoforms (95.5%) have SwissProt protein homologs. 26 isoforms have no detectable protein homolog. Top species: Mus musculus (705), Homo sapiens (43), Bos taurus (39), Rattus norvegicus (25), Gallus gallus (2).

High-Impact Novel Isoforms (150)

Gene	Impact	Details	ORF	Reads	CPM	SwissProt
Chn1 `transcript1043.2.nic`	Domain altered	Lost: zf-RING_9	complete	110	290.8	sp\|P30337\|CHIN_RAT 99.6% — N-chimaerin
TUBB2A `transcript270.13.nnic`	Domain altered	Lost: Tubulin_C	complete	44	116.3	sp\|P09203\|TBB1_CHICK 99.4% — Tubulin beta-1 chain
Il18 `transcript746.9.nic`	Domain altered	Gained: GT-A	complete	31	82.0	sp\|P70380\|IL18_MOUSE 100.0% — Interleukin-18
RBBP4 `transcript1265.4.nic`	Domain altered	Lost: Hexon	complete	29	76.7	sp\|Q3MHL3\|RBBP4_BOVIN 95.5% — Histone-binding protein RBBP4
Grb14 `transcript941.2.nic`	Domain altered	Lost: BPS, PH, Ubiquitin	complete	27	71.4	sp\|Q9JLM9\|GRB14_MOUSE 88.3% — Growth factor receptor-bound p...
Laptm4a `transcript70.12.nnic`	Domain altered	Lost: Circovir2_Orf4	complete	25	66.1	sp\|Q60961\|LAP4A_MOUSE 100.0% — Lysosomal-associated transmemb...
Ociad1 `transcript721.5.nic`	Domain altered	Lost: BCLP, C2H2-zf, YKT-like_S	complete	25	66.1	sp\|Q9CRD0\|OCAD1_MOUSE 100.0% — OCIA domain-containing protein...
Rbck1 `transcript2408.2.nnic`	Domain altered	Lost: Rad50_zn_hook, Ubiquitin, zf-RanBP	complete	22	58.2	sp\|Q9WUB0\|HOIL1_MOUSE 97.7% — RanBP-type and C3HC4-type zinc...
Lin7b `transcript1378.7.nic`	Domain altered	Gained: DUF2057	complete	18	47.6	sp\|O88951\|LIN7B_MOUSE 98.5% — Protein lin-7 homolog B
Bin1 `transcript227.18.nnic`	Domain altered	Lost: CK2S, DUF1664, Jnk-SapK_ap_N, OmpH, PTS_IIA, SNARE-fusion, TBD	complete	17	44.9	sp\|O08539\|BIN1_MOUSE 93.9% — Myc box-dependent-interacting ...
Cdc123 `transcript31.2.nnic`	Domain altered	Lost: Beta_propeller	complete	16	42.3	sp\|Q8CII2\|CD123_MOUSE 87.5% — Translation initiation factor ...
Cd81 `transcript3163.7.nnic`	Domain altered	Lost: DUF6768	complete	16	42.3	sp\|P35762\|CD81_MOUSE 100.0% — CD81 antigen
Tpd52l2 `transcript3228.2.nic`	Domain altered	Lost: DUF4164, Glyco_hydro_36, P-loop_NTPase, VBS-like	complete	15	39.7	sp\|Q9CYZ2\|TPD54_MOUSE 87.3% — Tumor protein D54
Khdrbs3 `transcript412.15.nnic`	Domain altered	Lost: FSA_C, STAR_dimer	complete	15	39.7	sp\|Q9R226\|KHDR3_MOUSE 100.0% — KH domain-containing, RNA-bind...
Gnptg `transcript428.17.nic`	Domain altered	Lost: AltA1	complete	15	39.7	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
PDLIM7 `transcript715.13.nic`	Domain altered +NMD	Lost: DUF4749, Rv0078B	complete	13	34.4	sp\|Q3TJD7\|PDLI7_MOUSE 100.0% — PDZ and LIM domain protein 7
Cibar1 `transcript72.4.nic`	Domain altered	Lost: DUF6447, Pec_lyase-like	complete	13	34.4	sp\|Q8BP22\|CBAR1_MOUSE 96.2% — CBY1-interacting BAR domain-co...
TSC22D1 `transcript1187.14.nnic`	Domain altered +NMD	Lost: DUF2946	complete	11	29.1	sp\|Q3MHL6\|T22D1_BOVIN 98.6% — TSC22 domain family protein 1
Psmb10 `transcript1601.8.nic`	Domain altered	Gained: 6PGD_C	complete	11	29.1	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Ubc `transcript1634.5.nnic`	Domain altered	Gained: DUF6393	complete	11	29.1	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Ociad1 `transcript730.5.nnic`	Domain altered +NMD	Lost: BCLP, C2H2-zf, YKT-like_S	complete	11	29.1	sp\|Q9CRD0\|OCAD1_MOUSE 100.0% — OCIA domain-containing protein...
TSC22D1 `transcript1186.14.nnic`	Domain altered +NMD	Lost: DUF2946	complete	10	26.4	sp\|Q3MHL6\|T22D1_BOVIN 98.6% — TSC22 domain family protein 1
Thoc7 `transcript48.14.nnic`	Domain altered	Gained: Pec_lyase-like, Tup_N, V_ATPase_I	complete	10	26.4	sp\|Q7TMY4\|THOC7_MOUSE 100.0% — THO complex subunit 7 homolog
St6galnac6 `transcript694.2.nnic`	Domain altered	Lost: Cytochrome-c	complete	10	26.4	sp\|Q9JM95\|SIA7F_MOUSE 100.0% — Alpha-N-acetylgalactosaminide ...
TSC22D1 `transcript1197.14.nnic`	Domain altered +NMD	Lost: DUF2946	complete	9	23.8	sp\|Q3MHL6\|T22D1_BOVIN 98.6% — TSC22 domain family protein 1
Cops4 `transcript925.5.nic`	Domain altered	Lost: YabA	complete	9	23.8	sp\|O88544\|CSN4_MOUSE 100.0% — COP9 signalosome complex subun...
Cct5 `transcript109.15.nic`	Domain altered	Lost: E-set	complete	8	21.2	sp\|P80316\|TCPE_MOUSE 100.0% — T-complex protein 1 subunit ep...
Nap1l1 `transcript1373.10.nnic`	Domain altered	Gained: SH3, TPR	complete	8	21.2	sp\|P28656\|NP1L1_MOUSE 99.4% — Nucleosome assembly protein 1-...
Acd `transcript1558.8.nic`	Domain altered	Gained: OGFr_III	complete	8	21.2	sp\|Q5EE38\|ACD_MOUSE 100.0% — Adrenocortical dysplasia prote...
Afg1l `transcript267.10.nnic`	Domain altered	Lost: DUF2075	complete	8	21.2	sp\|Q3V384\|AFG1L_MOUSE 99.2% — AFG1-like ATPase
Bax `transcript1397.7.nnic`	Domain altered	Lost: CF222	complete	7	18.5	sp\|Q07813\|BAX_MOUSE 100.0% — Apoptosis regulator BAX
Polb `transcript188.8.nnic`	Domain altered	Lost: HHH, WSN	complete	7	18.5	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Hlf `transcript1956.11.nic`	Domain altered	Gained: DUF4140, Ish1	complete	7	18.5	sp\|Q8BW74\|HLF_MOUSE 100.0% — Hepatic leukemia factor
Fastkd3 `transcript885.13.nic`	Domain altered	Lost: FAST_2, TPR	complete	7	18.5	sp\|Q8BSN9\|FAKD3_MOUSE 100.0% — FAST kinase domain-containing ...
Mrps15 `transcript1121.4.nic`	Domain altered	Lost: GPS2_interact	complete	6	15.9	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Rab4a `transcript1999.8.nic`	Domain altered	Lost: DUF2075	complete	6	15.9	sp\|P56371\|RAB4A_MOUSE 100.0% — Ras-related protein Rab-4A
Myl4 `transcript2538.11.nnic`	Domain altered	Gained: HTH	complete	6	15.9	sp\|P09541\|MYL4_MOUSE 100.0% — Myosin light chain 4
Hey1 `transcript35.3.nic`	Domain altered	Lost: HTH	complete	6	15.9	sp\|Q9WV93\|HEY1_MOUSE 99.6% — Hairy/enhancer-of-split relate...
Nmd3 `transcript377.3.nnic`	Domain altered	Lost: Atrophin-1, DUF3915, DUF4637, Zn_Beta_Ribbon	complete	6	15.9	sp\|Q99L48\|NMD3_MOUSE 91.1% — 60S ribosomal export protein N...
Gnptg `transcript436.17.nic`	Domain altered	Lost: AltA1	complete	6	15.9	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Sh3glb2 `transcript557.2.nnic`	Domain altered	Lost: SH3, bZIP	complete	6	15.9	sp\|Q5PPJ9\|SHLB2_RAT 98.6% — Endophilin-B2
CPLX2 `transcript558.13.nnic`	Domain altered +NMD	Lost: DUF2838	complete	6	15.9	sp\|P84088\|CPLX2_BOVIN 100.0% — Complexin-2
Pbx4 `transcript582.8.nic`	Domain altered	Lost: Carbpep_Y_N, DUF1479, HTH, P-loop_NTPase, YdaS_antitoxin	complete	6	15.9	sp\|Q99NE9\|PBX4_MOUSE 36.5% — Pre-B-cell leukemia transcript...
Rrp1 `transcript717.10.nic`	Domain altered	Lost: DUF4391, MMS19_N	complete	6	15.9	sp\|P56183\|RRP1_MOUSE 100.0% — Ribosomal RNA processing prote...
Ykt6 `transcript141.11.nnic`	Domain altered	Lost: SNARE-fusion	complete	5	13.2	sp\|Q9CQW1\|YKT6_MOUSE 100.0% — Synaptobrevin homolog YKT6
Psmb10 `transcript1602.8.nic`	Domain altered	Gained: 6PGD_C	complete	5	13.2	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
ACTL6B `transcript1959.5.nic`	Domain altered	Lost: Rep	complete	5	13.2	sp\|A4FUX8\|ACL6B_BOVIN 100.0% — Actin-like protein 6B
Mrpl48 `transcript2026.7.nic`	Domain altered	Gained: E-set	complete	5	13.2	sp\|Q8JZS9\|RM48_MOUSE 97.7% — Large ribosomal subunit protei...
Gabrd `transcript2055.4.nnic`	Domain altered	Lost: PsbY	complete	5	13.2	sp\|P22933\|GBRD_MOUSE 88.4% — Gamma-aminobutyric acid recept...
Igfbp4 `transcript2201.11.nnic`	Domain altered	Lost: GF_recep_C-rich	complete	5	13.2	sp\|P47879\|IBP4_MOUSE 100.0% — Insulin-like growth factor-bin...
Dctn6 `transcript312.8.nic`	Domain altered	Lost: Fucokinase	complete	5	13.2	sp\|Q9WUB4\|DCTN6_MOUSE 100.0% — Dynactin subunit 6
Dab1 `transcript709.4.nic`	Domain altered	Gained: Globin	complete	5	13.2	sp\|Q8CJH2\|DAB1_RAT 99.0% — Disabled homolog 1
Dab1 `transcript712.4.nic`	Domain altered	Gained: Globin	complete	5	13.2	sp\|Q8CJH2\|DAB1_RAT 99.5% — Disabled homolog 1
Nol12 `transcript756.15.nic`	Domain altered	Lost: ALC	complete	5	13.2	sp\|Q8BG17\|NOL12_MOUSE 89.9% — Nucleolar protein 12
Tpt1 `transcript1162.14.nnic`	Domain altered	Lost: DUF6613	complete	4	10.6	sp\|P63028\|TCTP_MOUSE 100.0% — Translationally-controlled tum...
Nme7 `transcript1410.1.nnic`	Domain altered	Lost: PH	complete	4	10.6	sp\|Q9QXL8\|NDK7_MOUSE 100.0% — Nucleoside diphosphate kinase ...
Ak5 `transcript1461.3.nnic`	Domain altered	Lost: MRP-S22	complete	4	10.6	sp\|Q920P5\|KAD5_MOUSE 100.0% — Adenylate kinase isoenzyme 5
Htatip2 `transcript1526.7.nnic`	Domain altered	Lost: Periplas_BP	complete	4	10.6	sp\|Q9Z2G9\|HTAI2_MOUSE 100.0% — Protein HTATIP2
Pard6a `transcript1580.8.nic`	Domain altered	Lost: Ubiquitin	complete	4	10.6	sp\|Q9Z101\|PAR6A_MOUSE 100.0% — Partitioning defective 6 homol...
Ubc `transcript1618.5.nnic`	Domain altered	Gained: DUF6393	complete	4	10.6	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Polb `transcript181.8.nnic`	Domain altered	Lost: HHH, WSN	complete	4	10.6	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Blvra `transcript1819.2.nnic`	Domain altered	Lost: NADP_Rossmann, SAM	complete	4	10.6	sp\|Q9CY64\|BIEA_MOUSE 100.0% — Biliverdin reductase A
Smap `transcript2313.7.nnic`	Domain altered	Lost: BUD22, HAD, Nop14, Triple_barrel	complete	4	10.6	sp\|Q3MHL8\|SMAP_BOVIN 98.9% — Small acidic protein
Becn1 `transcript2353.11.nic`	Domain altered	Lost: PP1c_bdg	complete	4	10.6	sp\|O88597\|BECN1_MOUSE 100.0% — Beclin-1
Cog7 `transcript2414.7.nic`	Domain altered	Lost: AbiGi, COG5, FlgN_FLiT	complete	4	10.6	sp\|Q3UM29\|COG7_MOUSE 100.0% — Conserved oligomeric Golgi com...
Nsmce1 `transcript2461.7.nnic`	Domain altered	Gained: AbiJ_NTD3, TMEMspv1-c74-12, TraH	complete	4	10.6	sp\|Q9D720\|NSE1_MOUSE 95.1% — Non-structural maintenance of ...
Gnptg `transcript431.17.nnic`	Domain altered	Lost: AltA1	complete	4	10.6	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Bcap31 `transcript504.X.nnic`	Domain altered	Gained: DoxD-like, GT-C, LMBR1	complete	4	10.6	sp\|Q61335\|BAP31_MOUSE 100.0% — B-cell receptor-associated pro...
Rgs3 `transcript553.4.nic`	Domain altered	Lost: DUF4441	complete	4	10.6	sp\|Q9DC04\|RGS3_MOUSE 100.0% — Regulator of G-protein signali...
Kxd1 `transcript691.8.nnic`	Domain altered	Lost: Sde2_N_Ubi, TolA_bind_tri, Ubiquitin, Zn_Beta_Ribbon	complete	4	10.6	sp\|Q80XH1\|KXDL1_MOUSE 71.2% — KxDL motif-containing protein ...
Sdr39u1 `transcript782.14.nic`	Domain altered	Lost: NADP_Rossmann	complete	4	10.6	sp\|Q5M8N4\|D39U1_MOUSE 100.0% — Epimerase family protein SDR39...
Cops4 `transcript921.5.nic`	Domain altered	Lost: YabA	complete	4	10.6	sp\|O88544\|CSN4_MOUSE 100.0% — COP9 signalosome complex subun...
Mrps15 `transcript1122.4.nnic`	Domain altered	Lost: DUF272, DUF4140, DUF724, GPS2_interact, MIX23, PIN	complete	3	7.9	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Setd3 `transcript1154.12.nic`	Domain altered	Lost: OB_enterotoxin	complete	3	7.9	sp\|Q91WC0\|SETD3_MOUSE 100.0% — Actin-histidine N-methyltransf...
Psmb2 `transcript1160.4.nnic`	Domain altered	Gained: TPR	complete	3	7.9	sp\|Q9R1P3\|PSB2_MOUSE 79.6% — Proteasome subunit beta type-2
Tmx2 `transcript1164.2.nnic`	Domain altered	Lost: pap2	complete	3	7.9	sp\|Q9D710\|TMX2_MOUSE 100.0% — Thioredoxin-related transmembr...
H2-Q10 `transcript1214.17.nic`	Domain altered	Lost: APC, Cadherin_C_2, Mucin	complete	3	7.9	sp\|P01898\|HA10_MOUSE 59.7% — H-2 class I histocompatibility...
Plpp1 `transcript1240.13.nnic`	Domain altered	Lost: GT-C	complete	3	7.9	sp\|Q61469\|PLPP1_MOUSE 98.9% — Phospholipid phosphatase 1
Ubac2 `transcript1335.14.nnic`	Domain altered	Gained: SH3	complete	3	7.9	sp\|Q8R1K1\|UBAC2_MOUSE 97.8% — Ubiquitin-associated domain-co...
Tradd `transcript1516.8.nic`	Domain altered	Gained: Cystine-knot	complete	3	7.9	sp\|Q3U0V2\|TRADD_MOUSE 99.4% — Tumor necrosis factor receptor...
Poc1a `transcript1787.9.nnic`	Domain altered	Lost: NTN, Nup160	complete	3	7.9	sp\|Q8JZX3\|POC1A_MOUSE 100.0% — POC1 centriolar protein homolo...
Pak1 `transcript1952.7.nnic`	Domain altered	Lost: DSHCT, DUF3741, PKinase	complete	3	7.9	sp\|O88643\|PAK1_MOUSE 96.7% — Serine/threonine-protein kinas...
ACTL6B `transcript1958.5.nic`	Domain altered	Lost: Rep	complete	3	7.9	sp\|A4FUX8\|ACL6B_BOVIN 100.0% — Actin-like protein 6B
Shisa5 `transcript1992.9.nic`	Domain altered	Gained: Fusion_gly	complete	3	7.9	sp\|Q9D7I0\|SHSA5_MOUSE 99.2% — Protein shisa-5
Ebp `transcript202.X.nnic`	Domain altered	Gained: PET122	complete	3	7.9	sp\|P70245\|EBP_MOUSE 100.0% — 3-beta-hydroxysteroid-Delta(8)...
Igfbp4 `transcript2202.11.nic`	Domain altered	Lost: GF_recep_C-rich	complete	3	7.9	sp\|P47879\|IBP4_MOUSE 100.0% — Insulin-like growth factor-bin...
Nrsn2 `transcript2418.2.nnic`	Domain altered	Gained: p35	complete	3	7.9	sp\|Q5HZK2\|NRSN2_MOUSE 100.0% — Neurensin-2
Hm13 `transcript2425.2.nnic`	Domain altered	Lost: GPCR_A	complete	3	7.9	sp\|Q9D8V0\|SPP_MOUSE 100.0% — Signal peptide peptidase
Commd1 `transcript300.11.nnic`	Domain altered	Lost: DUF4476	complete	3	7.9	sp\|Q8K4M5\|COMD1_MOUSE 100.0% — COMM domain-containing protein...
Slc35a4 `transcript391.18.nic`	Domain altered	Lost: DUF4535	complete	3	7.9	sp\|Q9D321\|S35A4_MOUSE 100.0% — Probable UDP-sugar transporter...
Khdrbs3 `transcript406.15.nnic`	Domain altered	Lost: STAR_dimer	complete	3	7.9	sp\|Q9R226\|KHDR3_MOUSE 100.0% — KH domain-containing, RNA-bind...
Thoc7 `transcript41.14.nnic`	Domain altered	Gained: Pec_lyase-like, Tup_N, V_ATPase_I	complete	3	7.9	sp\|Q7TMY4\|THOC7_MOUSE 100.0% — THO complex subunit 7 homolog
Sirt5 `transcript424.13.nic`	Domain altered	Lost: Herpes_LMP1	complete	3	7.9	sp\|Q8K2C6\|SIR5_MOUSE 100.0% — NAD-dependent protein deacylas...
Prxl2a `transcript465.14.nic`	Domain altered	Lost: 2heme_cytochrom	complete	3	7.9	sp\|Q9CYH2\|PXL2A_MOUSE 100.0% — Peroxiredoxin-like 2A
Slc50a1 `transcript607.3.nic`	Domain altered +NMD	Lost: YhhN	complete	3	7.9	sp\|Q9CXK4\|SWET1_MOUSE 100.0% — Sugar transporter SWEET1
Ift74 `transcript648.4.nnic`	Domain altered	Gained: Crescentin	complete	3	7.9	sp\|Q8BKE9\|IFT74_MOUSE 96.7% — Intraflagellar transport prote...
Eefsec `transcript927.6.nic`	Domain altered	Lost: FliS_cochap	complete	3	7.9	sp\|Q9JHW4\|SELB_MOUSE 95.8% — Selenocysteine-specific elonga...
Coq2 `transcript931.5.nic`	Domain altered	Lost: DUF2798	complete	3	7.9	sp\|Q66JT7\|COQ2_MOUSE 100.0% — 4-hydroxybenzoate polyprenyltr...
Tmem38a `transcript969.8.nic`	Domain altered	Lost: LTXXQ-like	complete	3	7.9	sp\|Q3TMP8\|TM38A_MOUSE 100.0% — Trimeric intracellular cation ...
Mbp `transcript966.18.nnic`	ORF disrupted		5prime_partial	126	333.1	—
Ndrg2 `transcript577.14.nic`	ORF disrupted		5prime_partial	85	224.7	sp\|Q9QYG0\|NDRG2_MOUSE 96.2% — Protein NDRG2
Syp `transcript116.X.nnic`	ORF disrupted		5prime_partial	66	174.5	sp\|Q62277\|SYPH_MOUSE 99.3% — Synaptophysin
HPCA `transcript1218.4.nic`	ORF disrupted		5prime_partial	50	132.2	sp\|Q4PL64\|HPCA_BOVIN 100.0% — Neuron-specific calcium-bindin...
Slc25a4 `transcript421.8.nnic`	ORF disrupted		5prime_partial	46	121.6	sp\|P48962\|ADT1_MOUSE 100.0% — ADP/ATP translocase 1
Stx4 `transcript2769.7.nic`	ORF disrupted	Lost: COG2, Csm1_N, DUF3671, DUF3698, E-set, ETRAMP, His_Kinase_A, PRP1_N, T2SSF, TPR, TPR_MLP1_2, Vac_Fusion Gained: GOLD-like	3prime_partial	44	116.3	sp\|P70452\|STX4_MOUSE 100.0% — Syntaxin-4
Stoml2 `transcript370.4.nic`	ORF disrupted		5prime_partial	44	116.3	sp\|Q99JB2\|STML2_MOUSE 100.0% — Stomatin-like protein 2, mitoc...
YWHAE `transcript1541.11.nic`	ORF disrupted	Lost: Ta0938	5prime_partial	38	100.5	sp\|P62261\|1433E_BOVIN 100.0% — 14-3-3 protein epsilon
Ntan1 `transcript184.16.nic`	ORF disrupted		5prime_partial	28	74.0	sp\|Q64311\|NTAN1_MOUSE 100.0% — Protein N-terminal asparagine ...
Araf `transcript298.X.nic`	ORF disrupted	Gained: Rubredoxin_2	5prime_partial	23	60.8	sp\|P04627\|ARAF_MOUSE 100.0% — Serine/threonine-protein kinas...
Mmd2 `transcript2165.5.nnic`	ORF disrupted	Lost: DUF2842 Gained: E-set, Ferlin_C	5prime_partial	20	52.9	sp\|Q8R189\|PAQRA_MOUSE 100.0% — Monocyte to macrophage differe...
Prkar1a `transcript2646.11.nic`	ORF disrupted		5prime_partial	20	52.9	sp\|Q9DBC7\|KAP0_MOUSE 100.0% — cAMP-dependent protein kinase ...
Sparcl1 `transcript1011.5.nic`	ORF disrupted		3prime_partial	19	50.2	sp\|P70663\|SPRL1_MOUSE 100.0% — SPARC-like protein 1
Ephx4 `transcript1024.5.nic`	ORF disrupted		5prime_partial	18	47.6	sp\|Q6IE26\|EPHX4_MOUSE 99.0% — Epoxide hydrolase 4
Tmem198 `transcript673.1.nic`	ORF disrupted		5prime_partial	16	42.3	sp\|Q8BG75\|TM198_MOUSE 100.0% — Transmembrane protein 198
Ntan1 `transcript188.16.nic`	ORF disrupted		5prime_partial	14	37.0	sp\|Q64311\|NTAN1_MOUSE 83.3% — Protein N-terminal asparagine ...
Cryzl1 `transcript1066.16.nic`	ORF disrupted		5prime_partial	13	34.4	sp\|Q921W4\|QORL1_MOUSE 100.0% — Quinone oxidoreductase-like pr...
Bcas1 `transcript2907.2.nnic`	ORF disrupted		5prime_partial	13	34.4	sp\|Q80YN3\|BCAS1_MOUSE 85.6% — Breast carcinoma-amplified seq...
P33monox `transcript579.13.nic`	ORF disrupted		5prime_partial	13	34.4	sp\|Q9DBN4\|P33MX_MOUSE 100.0% — Putative monooxygenase p33MONO...
Pcsk1n `transcript183.X.nnic`	ORF disrupted	Lost: FUSC	5prime_partial	12	31.7	sp\|Q9QXV0\|PCS1N_MOUSE 99.5% — ProSAAS
Fam32a `transcript946.8.nic`	ORF disrupted		5prime_partial	12	31.7	sp\|Q9CR80\|FA32A_MOUSE 100.0% — Protein FAM32A
Zdhhc16 `transcript950.19.nic`	ORF disrupted		3prime_partial	12	31.7	sp\|Q969W1\|ZDH16_HUMAN 98.9% — Palmitoyltransferase ZDHHC16
Gipc1 `transcript1104.8.nic`	ORF disrupted		5prime_partial	11	29.1	sp\|Q9Z0G0\|GIPC1_MOUSE 99.4% — PDZ domain-containing protein ...
Rtkn `transcript726.6.nic`	ORF disrupted		5prime_partial	11	29.1	sp\|Q8C6B2\|RTKN_MOUSE 95.5% — Rhotekin
Ccdc106 `transcript138.7.nic`	ORF disrupted	Gained: E-set	5prime_partial	10	26.4	sp\|Q3ULM0\|CC106_MOUSE 100.0% — Coiled-coil domain-containing ...
Commd8 `transcript715.5.nnic`	ORF disrupted		5prime_partial	10	26.4	sp\|Q9CZG3\|COMD8_MOUSE 99.4% — COMM domain-containing protein...
TXNL4A `transcript915.18.nnic`	ORF disrupted		3prime_partial	10	26.4	—
Cuedc2 `transcript1091.19.nnic`	ORF disrupted		3prime_partial	9	23.8	sp\|Q9CXX9\|CUED2_MOUSE 99.2% — CUE domain-containing protein ...
Nat14 `transcript122.7.nnic`	ORF disrupted		5prime_partial	9	23.8	sp\|Q8BVG8\|NAT14_MOUSE 100.0% — Probable N-acetyltransferase 1...
Paip2 `transcript340.18.nnic`	ORF disrupted	Gained: DUF3911	5prime_partial	9	23.8	sp\|Q3ZC67\|PAIP2_BOVIN 100.0% — Polyadenylate-binding protein-...
Chmp3 `transcript611.6.nnic`	ORF disrupted	Lost: DUF3915, PSD4, SPOUT Gained: Zn_Beta_Ribbon, zf-ZPR1	5prime_partial	9	23.8	sp\|Q9CQ10\|CHMP3_MOUSE 86.0% — Charged multivesicular body pr...
Rtkn `transcript727.6.nic`	ORF disrupted		5prime_partial	9	23.8	sp\|Q8C6B2\|RTKN_MOUSE 100.0% — Rhotekin
Ak6 `transcript1153.13.nic`	ORF disrupted	Lost: AAA_lid, DUF2125, DUF3245, DUF4611, Histone	5prime_partial	8	21.2	sp\|Q8VCP8\|KAD6_MOUSE 100.0% — Adenylate kinase isoenzyme 6
Snx12 `transcript678.X.nnic`	ORF disrupted		5prime_partial	8	21.2	sp\|Q9UMY4\|SNX12_HUMAN 100.0% — Sorting nexin-12
Cuedc2 `transcript1087.19.nnic`	ORF disrupted		3prime_partial	7	18.5	sp\|Q9CXX9\|CUED2_MOUSE 91.6% — CUE domain-containing protein ...
Cluap1 `transcript11.16.nnic`	ORF disrupted	Lost: BUD22, CENP-B_dimeris Gained: PBP1_TM	5prime_partial	7	18.5	sp\|Q8R3P7\|CLUA1_MOUSE 93.8% — Clusterin-associated protein 1
Pacsin3 `transcript1257.2.nic`	ORF disrupted		5prime_partial	7	18.5	sp\|Q99JB8\|PACN3_MOUSE 100.0% — Protein kinase C and casein ki...
Cadm1 `transcript712.9.nic`	ORF disrupted	Lost: DUF3439, MGC-24, Mid2, Mucin, SSP160	5prime_partial	7	18.5	sp\|Q8R5M8\|CADM1_MOUSE 90.5% — Cell adhesion molecule 1
Nfu1 `transcript868.6.nic`	ORF disrupted		5prime_partial	7	18.5	sp\|Q9QZ23\|NFU1_MOUSE 77.7% — NFU1 iron-sulfur cluster scaff...
Atxn10 `transcript1102.15.nic`	ORF disrupted		3prime_partial	6	15.9	sp\|P28658\|ATX10_MOUSE 100.0% — Ataxin-10
Sugt1 `transcript1248.14.nnic`	ORF disrupted		5prime_partial	6	15.9	sp\|Q9CX34\|SGT1_MOUSE 100.0% — Protein SGT1 homolog
Mog `transcript1253.17.nnic`	ORF disrupted	Lost: PF	5prime_partial	6	15.9	sp\|Q61885\|MOG_MOUSE 97.1% — Myelin-oligodendrocyte glycopr...
Gabrd `transcript2056.4.nic`	ORF disrupted	Lost: PsbY	3prime_partial	6	15.9	sp\|P22933\|GBRD_MOUSE 100.0% — Gamma-aminobutyric acid recept...
Serpinb6 `transcript233.13.nic`	ORF disrupted		5prime_partial	6	15.9	sp\|Q60854\|SPB6_MOUSE 100.0% — Serpin B6
Mapk3 `transcript2521.7.nic`	ORF disrupted		5prime_partial	6	15.9	sp\|Q63844\|MK03_MOUSE 100.0% — Mitogen-activated protein kina...
YPEL3 `transcript2584.7.nic`	ORF disrupted		5prime_partial	6	15.9	sp\|A6QPH8\|YPEL3_BOVIN 100.0% — Protein yippee-like 3
Ankrd13d `transcript104.19.nnic`	ORF disrupted	Lost: Ank	5prime_partial	5	13.2	sp\|Q6PD24\|AN13D_MOUSE 100.0% — Ankyrin repeat domain-containi...
Cnpy3 `transcript1467.17.nnic`	ORF disrupted	Lost: TBK1_CCD1	5prime_partial	5	13.2	sp\|Q9DAU1\|CNPY3_MOUSE 97.9% — Protein canopy homolog 3
Ntan1 `transcript181.16.nic`	ORF disrupted		5prime_partial	5	13.2	sp\|Q64311\|NTAN1_MOUSE 84.7% — Protein N-terminal asparagine ...
Septin4 `transcript1890.11.nic`	ORF disrupted		5prime_partial	5	13.2	sp\|P28661\|SEPT4_MOUSE 99.6% — Septin-4
RPL19 `transcript2136.11.nnic`	ORF disrupted		5prime_partial	5	13.2	sp\|Q3T0W9\|RL19_BOVIN 97.9% — Large ribosomal subunit protei...

Frame Impact Classification

Functional Diversity per Gene

Top Pfam Domains

Domain Architectures per Gene

Annotation QC: Pfam Artifact Detection

Related Pfam families are grouped into clans; proteins with anomalously high domain density (structural protein artifacts) are flagged separately. The cleaned set is used for all downstream analysis.

Original DV Genes

866

After QC Filtering

617

Removed (Artifacts)

249

Flagged Details

154

Flagged Artifacts (154)

Gene	Gene Name	Reason	Arch (raw)	Arch (collapsed)	Core / Variable Domains
`ENSMUSG00000032366`	TPM4	density artifact	7	0	38 / 26
`ENSMUSG00000027940`	Tpm3	density artifact	3	0	43 / 12
`ENSMUSG00000014402`	Tsg101	density artifact	3	0	38 / 5
`ENSMUSG00000027506`	Tpd52	density artifact	3	0	28 / 12
`ENSMUSG00000026761`	Orc4	density artifact	3	0	43 / 12
`ENSMUSG00000026927`	Entr1	density artifact	3	0	27 / 1
`ENSMUSG00000007207`	Stx1a	density artifact	2	0	53 / 0
`ENSMUSG00000030805`	Stx4	density artifact	2	0	31 / 0
`ENSMUSG00000079477`	RAB7A	density artifact	2	0	26 / 0
`ENSMUSG00000002985`	Apoe	density artifact	2	0	42 / 0
`ENSMUSG00000019303`	Psmc3ip	density artifact	2	0	71 / 0
`ENSMUSG00000030603`	Psmc4	density artifact	2	0	36 / 0
`ENSMUSG00000129504`	Nefm	density artifact	2	0	33 / 0
`ENSMUSG00000069208`	Znf431	density artifact	2	0	29 / 0
`ENSMUSG00000001175`	CALM1	density artifact	2	0	31 / 0
`ENSMUSG00000059689`	Znf32	density artifact	2	0	40 / 0
`ENSMUSG00000026966`	Ssna1	density artifact	1	0	55 / 0
`ENSMUSG00000019370`	CALM1	density artifact	1	0	30 / 0
`ENSMUSG00000020486`	Septin4	clan collapse	4	1	13 / 3
`ENSMUSG00000038286`	Bphl	clan collapse	3	1	12 / 0
`ENSMUSG00000057236`	RBBP4	clan collapse	4	2	3 / 3
`ENSMUSG00000055720`	Ubl7	clan collapse	3	1	6 / 0
`ENSMUSG00000034209`	Rasl10a	clan collapse	3	1	4 / 3
`ENSMUSG00000026201`	Stk16	clan collapse	4	2	7 / 2
`ENSMUSG00000022971`	Ifnar2	clan collapse	2	1	4 / 0
`ENSMUSG00000078923`	Ube2v1	clan collapse	2	1	2 / 0
`ENSMUSG00000069835`	Sat2	clan collapse	2	1	8 / 0
`ENSMUSG00000068250`	Amn1	clan collapse	2	1	4 / 0
`ENSMUSG00000038374`	RBM8A	clan collapse	2	1	4 / 0
`ENSMUSG00000052752`	TRAF7	clan collapse	2	1	25 / 0
`ENSMUSG00000053565`	Eif3k	clan collapse	2	1	1 / 2
`ENSMUSG00000024516`	Sec11c	clan collapse	2	1	2 / 0
`ENSMUSG00000007603`	Dus3l	clan collapse	2	1	5 / 1
`ENSMUSG00000022024`	Sugt1	clan collapse	2	1	18 / 0
`ENSMUSG00000005150`	Wdr83	clan collapse	2	1	6 / 0
`ENSMUSG00000026972`	Arrdc1	clan collapse	2	1	4 / 0
`ENSMUSG00000040532`	Abhd11	clan collapse	2	1	10 / 4
`ENSMUSG00000024617`	CAMK2A	clan collapse	2	1	12 / 0
`ENSMUSG00000116594`	SEPTIN5	clan collapse	2	1	16 / 0
`ENSMUSG00000037373`	Ctbp1	clan collapse	2	1	6 / 0
`ENSMUSG00000037894`	H2AZ1	clan collapse	2	1	3 / 0
`ENSMUSG00000025470`	Znf511	clan collapse	2	1	5 / 0
`ENSMUSG00000021772`	Nkiras1	clan collapse	2	1	9 / 0
`ENSMUSG00000045136`	TUBB2B	clan collapse	2	1	3 / 0
`ENSMUSG00000039183`	Nubp2	clan collapse	2	1	2 / 19
`ENSMUSG00000040136`	Abcc8	clan collapse	2	1	11 / 0
`ENSMUSG00000058240`	Cryzl1	clan collapse	2	1	2 / 1
`ENSMUSG00000000253`	Gmpr	clan collapse	2	1	4 / 0
`ENSMUSG00000033429`	Mcee	clan collapse	2	1	5 / 0
`ENSMUSG00000047766`	Lrrc49	clan collapse	2	1	4 / 0
`ENSMUSG00000011658`	Fuz	clan collapse	2	1	3 / 0
`ENSMUSG00000087260`	Lamtor5	clan collapse	2	1	2 / 0
`ENSMUSG00000028683`	Eif2b3	clan collapse	2	1	7 / 0
`ENSMUSG00000049225`	Pdp1	clan collapse	2	1	1 / 1
`ENSMUSG00000032786`	Alas1	clan collapse	2	1	5 / 0
`ENSMUSG00000025499`	H-RAS	clan collapse	2	1	13 / 0
`ENSMUSG00000021111`	Papola	clan collapse	2	1	4 / 0
`ENSMUSG00000079657`	Rab26	clan collapse	2	1	17 / 0
`ENSMUSG00000032475`	Nck1	clan collapse	2	1	8 / 0
`ENSMUSG00000019977`	Hbs1l	clan collapse	2	1	8 / 0
`ENSMUSG00000028057`	Rit1	clan collapse	2	1	5 / 0
`ENSMUSG00000020873`	Slc35b1	clan collapse	2	1	5 / 0
`ENSMUSG00000017307`	Acot8	clan collapse	2	1	3 / 0
`ENSMUSG00000019054`	Fis1	clan collapse	2	1	8 / 0
`ENSMUSG00000024645`	Timm21	clan collapse	2	1	2 / 0
`ENSMUSG00000000711`	RAB5B	clan collapse	2	1	9 / 0
`ENSMUSG00000039826`	Trub2	clan collapse	2	1	8 / 0
`ENSMUSG00000026349`	Ccnt2	clan collapse	2	1	3 / 0
`ENSMUSG00000030815`	Phkg2	clan collapse	2	1	14 / 0
`ENSMUSG00000028954`	Nub1	clan collapse	2	1	11 / 0
`ENSMUSG00000023175`	Bsg	clan collapse	2	1	7 / 0
`ENSMUSG00000006728`	Cdk4	clan collapse	2	1	8 / 0
`ENSMUSG00000027998`	Plrg1	clan collapse	2	1	9 / 0
`ENSMUSG00000028099`	Polr3c	clan collapse	2	1	3 / 0
`ENSMUSG00000071379`	Hpcal1	clan collapse	2	1	11 / 0
`ENSMUSG00000002524`	Puf60	clan collapse	2	1	5 / 0
`ENSMUSG00000027523`	Gnas	clan collapse	2	1	7 / 0
`ENSMUSG00000020309`	Chac2	clan collapse	2	1	2 / 0
`ENSMUSG00000025736`	Jmjd8	clan collapse	2	1	5 / 0
`ENSMUSG00000025731`	Mettl26	clan collapse	2	1	6 / 1
`ENSMUSG00000031641`	Cbr4	clan collapse	2	1	6 / 0
`ENSMUSG00000021062`	Rab15	clan collapse	2	1	20 / 0
`ENSMUSG00000029538`	Srsf9	clan collapse	2	1	5 / 0
`ENSMUSG00000042831`	Alkbh6	clan collapse	2	1	1 / 1
`ENSMUSG00000006782`	Cnp	clan collapse	2	1	23 / 0
`ENSMUSG00000031505`	Naxd	clan collapse	2	1	3 / 0
`ENSMUSG00000029034`	Ints11	clan collapse	2	1	7 / 0
`ENSMUSG00000038975`	Rabggtb	clan collapse	2	1	3 / 0
`ENSMUSG00000022474`	Pmm1	clan collapse	2	1	3 / 0
`ENSMUSG00000003346`	Abhd17a	clan collapse	2	1	14 / 0
`ENSMUSG00000019173`	Rab5c	clan collapse	2	1	9 / 0
`ENSMUSG00000059974`	Ntm	clan collapse	2	1	12 / 0
`ENSMUSG00000004933`	Matk	clan collapse	2	1	9 / 0
`ENSMUSG00000044628`	Rnf208	clan collapse	2	1	10 / 0
`ENSMUSG00000045211`	Nudt18	clan collapse	2	1	2 / 0
`ENSMUSG00000028252`	Ccnc	clan collapse	2	1	4 / 0
`ENSMUSG00000030512`	SNRPA1	clan collapse	2	1	4 / 0
`ENSMUSG00000039048`	Foxred1	clan collapse	2	1	9 / 0
`ENSMUSG00000026825`	DNM1	clan collapse	2	1	6 / 0
`ENSMUSG00000032336`	Nptn	clan collapse	2	1	8 / 2
`ENSMUSG00000037062`	Sh3glb1	clan collapse	2	1	6 / 1
`ENSMUSG00000024217`	Snrpc	clan collapse	2	1	3 / 0
`ENSMUSG00000021982`	Cdadc1	clan collapse	2	1	4 / 0
`ENSMUSG00000036067`	Slc2a6	clan collapse	2	1	3 / 0
`ENSMUSG00000090841`	Myl6	clan collapse	2	1	6 / 0
`ENSMUSG00000022570`	Gfus	clan collapse	2	1	6 / 0
`ENSMUSG00000030701`	Plekhb1	clan collapse	2	1	3 / 0
`ENSMUSG00000039345`	Mettl22	clan collapse	2	1	6 / 0
`ENSMUSG00000024862`	KLC2	clan collapse	2	1	38 / 0
`ENSMUSG00000113262`	Dohh	clan collapse	2	1	6 / 0
`ENSMUSG00000057411`	Antkmt	clan collapse	2	1	4 / 1
`ENSMUSG00000073423`	Znf414	clan collapse	2	1	6 / 1
`ENSMUSG00000024878`	Zng1	clan collapse	2	1	15 / 0
`ENSMUSG00000026426`	Arl8a	clan collapse	2	1	8 / 0
`ENSMUSG00000063065`	Mapk3	clan collapse	2	1	9 / 0
`ENSMUSG00000042396`	Rbm7	clan collapse	2	1	4 / 0
`ENSMUSG00000000184`	Ccnd2	clan collapse	2	1	3 / 0
`ENSMUSG00000004032`	Gstm5	clan collapse	2	1	6 / 0
`ENSMUSG00000026172`	Bcs1l	clan collapse	2	1	22 / 2
`ENSMUSG00000019295`	Tmem129	clan collapse	2	1	4 / 0
`ENSMUSG00000024168`	Tmem204	clan collapse	2	1	7 / 0
`ENSMUSG00000077450`	Rab11b	clan collapse	2	1	24 / 0
`ENSMUSG00000052456`	Get3	clan collapse	2	1	15 / 0
`ENSMUSG00000030714`	Sgf29	clan collapse	2	1	4 / 0
`ENSMUSG00000093445`	Lrch4	clan collapse	2	1	7 / 0
`ENSMUSG00000063235`	Ptpmt1	clan collapse	2	1	5 / 0
`ENSMUSG00000022635`	Zcrb1	clan collapse	2	1	6 / 0
`ENSMUSG00000073422`	Hsd17b8	clan collapse	2	1	6 / 0
`ENSMUSG00000051730`	Mettl5	clan collapse	2	1	23 / 0
`ENSMUSG00000038671`	Arfrp1	clan collapse	2	1	9 / 0
`ENSMUSG00000070880`	Gad1	clan collapse	2	1	6 / 0
`ENSMUSG00000021094`	Dhrs7	clan collapse	2	1	5 / 0
`ENSMUSG00000024944`	Arl2	clan collapse	2	1	10 / 0
`ENSMUSG00000021548`	Ccnh	clan collapse	2	1	3 / 0
`ENSMUSG00000029012`	Orc5	clan collapse	2	1	8 / 0
`ENSMUSG00000025939`	Ube2w	clan collapse	2	1	3 / 0
`ENSMUSG00000042540`	Acot5	clan collapse	2	1	5 / 0
`ENSMUSG00000031144`	Syp	clan collapse	2	1	2 / 0
`ENSMUSG00000020385`	Clk4	clan collapse	2	1	5 / 0
`ENSMUSG00000051483`	Cbr1	clan collapse	2	1	5 / 0
`ENSMUSG00000017715`	Pgs1	clan collapse	2	1	3 / 0
`ENSMUSG00000040952`	Rps19	clan collapse	2	1	3 / 0
`ENSMUSG00000039382`	Wdr45	clan collapse	2	1	4 / 0
`ENSMUSG00000053483`	Usp21	clan collapse	2	1	3 / 0
`ENSMUSG00000042729`	Wdr74	clan collapse	2	1	4 / 0
`ENSMUSG00000015806`	Qdpr	clan collapse	2	1	5 / 0
`ENSMUSG00000006369`	Fbln1	clan collapse	2	1	6 / 0
`ENSMUSG00000013622`	Atraid	clan collapse	2	1	4 / 0
`ENSMUSG00000022453`	Naga	clan collapse	2	1	4 / 0
`ENSMUSG00000056367`	Actr3b	clan collapse	2	1	5 / 0
`ENSMUSG00000020571`	Pdia6	clan collapse	2	1	14 / 0
`ENSMUSG00000020612`	Prkar1a	clan collapse	2	1	4 / 0
`ENSMUSG00000046434`	HNRNPA1	clan collapse	2	1	6 / 0
`ENSMUSG00000053062`	Jam2	clan collapse	2	1	9 / 0

Domain Variants by Evidence Tier

Variable Domain Landscape

Total Variable Domains

359

Domain Loss Events

360

Domain Gain Events

Loss : Gain Ratio

15.0:1

Domain loss dominates gain ~15:1, consistent with alternative splicing predominantly removing exons encoding specific domains rather than inserting novel domain-encoding exons.

All Variable Domains (359)

Domain	DV Genes	Gain	Loss	Genes
`eIF2A`	3	0	3	GNB2, Poc1a, RBBP4
`DUF4407`	3	1	2	Dtnb, Ing4, Hnrnpc
`DUF1664`	3	0	3	Bin1, Ccpg1, Coro1a
`Ge1_WD40`	2	0	2	Fbxw2, Poc1a
`Prok-RING_4`	2	0	2	Mkrn1, Rnf13
`YabA`	2	0	2	Cops4, Hnrnpc
`DUF4200`	2	0	2	Ing4, Sybu
`Exonuc_VII_L`	2	0	2	Septin8, Sybu
`FAM76`	2	0	2	Srsf5, Sybu
`CDC45`	2	0	2	Aplp2, Smap
`Mucin`	2	0	2	Cadm1, H2-Q10
`AAA_16`	2	0	2	Ak6, Pstk
`AAA_25`	2	0	2	Ak6, Pstk
`Baculo_F`	2	0	2	Shisa5, Stx5
`Herpes_DNAp_acc`	2	0	2	Cacnb3, Eef1d
`Jnk-SapK_ap_N`	2	0	2	Bin1, Stx5
`Presenilin`	2	1	1	Cd47, RNPS1
`IGFBP`	2	0	2	Igfbp2, Igfbp4
`PPP4R2`	2	0	2	Anapc15, Apbb1
`G6B`	2	0	2	Ccdc106, Mrpl48
`ubiquitin`	2	0	2	Kxd1, Tecr
`Uso1_p115_C`	2	0	2	Bloc1s2, Tpd52l1
`LMBR1`	1	0	1	Psma3
`DUF155`	1	0	1	Tmed5
`Mtf2_C`	1	0	1	Tmed5
`Chromo`	1	0	1	Tmed5
`DUF3573`	1	0	1	Tmed5
`CHAP`	1	0	1	Fbxw2
`MASE3`	1	0	1	Hm13
`ANAPC4_WD40`	1	0	1	RBBP4
`NBCH_WD40`	1	0	1	RBBP4
`DUF2946`	1	0	1	TSC22D1
`YbgT_YccB`	1	0	1	Atp6v0b
`DUF3742`	1	0	1	Atp6v0b
`PP1c_bdg`	1	0	1	Becn1
`Ribosomal_S6`	1	0	1	Ppil2
`DNA_primase_lrg`	1	0	1	Rpl18a
`DNA_ligase_ZBD`	1	0	1	Rnf13
`Neur_chan_memb`	1	0	1	Rnf13
`PHD`	1	0	1	Rnf13
`zf-RING_4`	1	0	1	Rnf13
`CTU2`	1	0	1	Rnf13
`zf-rbx1`	1	0	1	Rnf13
`zf-C3HC4_3`	1	0	1	Rnf13
`HTH_63`	1	0	1	Rnf13
`zf-ANAPC11`	1	0	1	Rnf13
`RINGv`	1	0	1	Rnf13
`SID-1_RNA_chan`	1	0	1	Rnf13
`zf-RING_16`	1	0	1	Rnf13
`zf-C3H2C3`	1	0	1	Rnf13
`Malic_M`	1	0	1	Me3
`DUF418`	1	0	1	Plpp1
`COX4`	1	0	1	Plpp1
`DUF6541`	1	0	1	Plpp1
`CCER1`	1	0	1	Dnajc7
`IspA`	1	0	1	Dnajc7
`TPR-S`	1	0	1	Dnajc7
`TPR_14`	1	0	1	Cops4
`Redoxin`	1	0	1	Rpl34
`DUF641`	1	0	1	Sybu
`BST2`	1	0	1	Sybu
`GPS2_interact`	1	0	1	Mrps15
`DUF3911`	1	0	1	Paip2
`DUF4748`	1	0	1	Pnkd
`PA26`	1	1	0	Hnrnpc
`Nup88`	1	1	0	Hnrnpc
`TMPIT`	1	1	0	Hnrnpc
`Cytadhesin_P30`	1	1	0	Hnrnpc
`FUT8_N_cat`	1	0	1	Hnrnpc
`CDC24`	1	0	1	Aplp2
`DabA`	1	0	1	Aplp2
`Kunitz_BPTI`	1	0	1	Aplp2
`DUF5069`	1	0	1	Psma7
`gpW`	1	0	1	Psma7
`PHD_4`	1	0	1	Phf23
`Ribosomal_60s`	1	0	1	Phf23
`Nha1_C`	1	0	1	Phf23
`tRNA_anti-like`	1	0	1	Nars1
`tRNA_anti_2`	1	0	1	Nars1
`LPP20`	1	0	1	Stau1
`CDP-OH_P_tran_2`	1	0	1	Mtch2
`FXR_C3`	1	0	1	Fxr1
`eIF-3_zeta`	1	0	1	Fxr1
`DUF4629`	1	0	1	Fxr1
`GlcNAc-1_reg`	1	0	1	Tcp1
`PAP2_3`	1	0	1	Tmx2
`Phage_holin_3_6`	1	0	1	Rtn4
`PCAF_N`	1	0	1	Pea15
`Tfb5`	1	0	1	Ndufab1
`PRY`	1	0	1	Trim9
`SPRY`	1	0	1	Trim9
`VP1_VP3`	1	0	1	Serbp1
`DAG_kinase_N`	1	0	1	Zfand3
`Nucleo_P87`	1	0	1	Zfand3
`FYVE`	1	0	1	Zfand3
`AA_permease_2`	1	0	1	H2-Q10
`Cadherin_C_2`	1	0	1	H2-Q10
`DUF4164`	1	1	0	Tpd52l2
`Glyco_hydro_36`	1	1	0	Tpd52l2
`AAA_13`	1	0	1	Tpd52l2
`AAA_22`	1	0	1	Pstk
`AAA_33`	1	0	1	Pstk
`AAA_18`	1	0	1	Pstk
`DO-GTPase1`	1	0	1	Pstk
`MeaB`	1	0	1	Pstk
`DEAD_assoc`	1	0	1	Pstk
`Zeta_toxin`	1	0	1	Pstk
`ParA`	1	0	1	Pstk
`APS_kinase`	1	0	1	Pstk
`BUD22`	1	0	1	Smap
`Nop14`	1	0	1	Smap
`RNA_polI_A34`	1	0	1	Smap
`YrbL-PhoP_reg`	1	0	1	RPS24
`CaM_bind`	1	0	1	GNB2
`DUF3584`	1	0	1	ENSMUSG00000063236
`PGC7_Stella`	1	0	1	ENSMUSG00000063236
`PolC_DP2`	1	0	1	ENSMUSG00000063236
`Cytochrom_D1`	1	0	1	Poc1a
`WD40_like`	1	0	1	Poc1a
`Proteasome_A_N`	1	0	1	Poc1a
`Nup160`	1	0	1	Poc1a
`YkyA`	1	0	1	Dtnb
`SOGA`	1	0	1	Dtnb
`Seryl_tRNA_N`	1	0	1	Dtnb
`FapA`	1	0	1	Dtnb
`FUSC`	1	0	1	Dtnb
`GET2`	1	0	1	Dtnb
`ZZ`	1	0	1	Dtnb
`DUF4988`	1	0	1	Dtnb
`LIP`	1	0	1	Abhd12
`BD-FAE`	1	0	1	Abhd12
`Abhydrolase_2`	1	0	1	Abhd12
`Peptidase_S9`	1	0	1	Abhd12
`RAP`	1	0	1	Abhd12
`Peptidase_M90`	1	0	1	KCTD17
`FXMRP1_C_core`	1	0	1	Drap1
`ZapB`	1	0	1	Fgfr1op2
`FIN1`	1	0	1	Fgfr1op2
`TPR_MLP1_2`	1	0	1	Fgfr1op2
`Occludin_ELL`	1	0	1	Fgfr1op2
`Sec20`	1	0	1	Fgfr1op2
`Snapin_Pallidin`	1	0	1	Fgfr1op2
`Prefoldin_2`	1	0	1	Fgfr1op2
`MAT1`	1	0	1	Fgfr1op2
`Phage_GP20`	1	0	1	Fgfr1op2
`DUF4446`	1	0	1	Fgfr1op2
`CHD5`	1	0	1	Fgfr1op2
`betaPIX_CC`	1	0	1	Fgfr1op2
`LUC7`	1	0	1	Fgfr1op2
`bZIP_1`	1	0	1	Fgfr1op2
`DUF2570`	1	0	1	Fgfr1op2
`WXG100`	1	0	1	Fgfr1op2
`Cauli_AT`	1	0	1	Fgfr1op2
`TolA_bind_tri`	1	0	1	Fgfr1op2
`UPF0242`	1	0	1	Fgfr1op2
`DiS_P_DiS`	1	0	1	YPEL3
`Elf1`	1	0	1	YPEL3
`GRP`	1	0	1	Raly
`RhlB`	1	0	1	Raly
`Filament_head`	1	0	1	Raly
`Herpes_capsid`	1	0	1	Raly
`DUF4193`	1	0	1	Shisa4
`TMF_TATA_bd`	1	1	0	Klc1
`MIT`	1	1	0	Klc1
`TSP_1`	1	0	1	Rnaseh2a
`PMSI1`	1	0	1	Rnaseh2a
`TBD`	1	0	1	Bin1
`BAR_3`	1	0	1	Bin1
`V-SNARE`	1	0	1	Bin1
`OmpH`	1	0	1	Bin1
`Arfaptin`	1	0	1	Bin1
`CK2S`	1	0	1	Bin1
`Neurensin`	1	0	1	Tmem134
`DUF2207`	1	0	1	Tmem134
`DUF3040`	1	0	1	Tmem134
`DUF308`	1	0	1	Tmem134
`SdpI`	1	0	1	Tmem134
`PAP2_C`	1	0	1	Plpp5
`LBP_C`	1	0	1	Plpp5
`CcmH`	1	0	1	SMIM29
`DUF2371`	1	0	1	SMIM29
`BCCT`	1	0	1	SMIM29
`COX14`	1	0	1	SMIM29
`PaREP1`	1	0	1	SMIM29
`ABC2_membrane_3`	1	0	1	SMIM29
`DUF2457`	1	1	0	RNPS1
`DUF4191`	1	0	1	Cacfd1
`DUF2231`	1	0	1	Cacfd1
`Herpes_LMP1`	1	0	1	Sirt5
`ANF_receptor`	1	0	1	Htatip2
`LapA_dom`	1	0	1	Gpm6b
`DUF6304`	1	0	1	Rundc3a
`RED_N`	1	0	1	Rbm5
`DASH_Spc19`	1	0	1	Ing4
`IFP_35_N`	1	0	1	Ing4
`DASH_Dad2`	1	0	1	Ing4
`PHD_2`	1	0	1	Ing4
`AP3D1`	1	0	1	Ing4
`DUF1376`	1	0	1	Ing4
`ABC_tran_CTD`	1	0	1	Ing4
`DUF1192`	1	0	1	Ing4
`HCMV_UL139`	1	0	1	Ing4
`AICARFT_IMPCHas`	1	0	1	Rsph3a
`SelR`	1	0	1	Slirp
`Pox_F16`	1	0	1	Mettl23
`PrmA`	1	0	1	Mettl23
`DUF6161`	1	0	1	CLTA
`FAM184`	1	0	1	CLTA
`CLZ`	1	0	1	Coro1a
`GBP_C`	1	0	1	Stmn4
`CCDC84`	1	0	1	Stmn4
`AAA_23`	1	0	1	Stmn4
`Cwf_Cwc_15`	1	0	1	Anapc15
`RXT2_N`	1	0	1	Anapc15
`Na_trans_assoc`	1	0	1	Anapc15
`DUF4746`	1	0	1	Anapc15
`EBV-NA3`	1	0	1	Anapc15
`NOA36`	1	0	1	Anapc15
`Drc1-Sld2`	1	0	1	Anapc15
`Nop53`	1	0	1	Anapc15
`Autophagy_act_C`	1	0	1	Anapc15
`Ig_2`	1	0	1	Scn1b
`ig`	1	0	1	Scn1b
`DUF6264`	1	0	1	Scn1b
`Protocadherin`	1	0	1	Scn1b
`DUF5326`	1	0	1	Scn1b
`ATG16`	1	0	1	Septin8
`Microtub_bd`	1	0	1	Septin8
`PRR20`	1	0	1	Fam241b
`DUF4632`	1	0	1	Ddx39a
`YhhN`	1	0	1	Tusc3
`Bax1-I`	1	0	1	Tusc3
`DUF6350`	1	0	1	Tusc3
`Yip1`	1	0	1	Tusc3
`DUF349`	1	0	1	Hmox2
`RE_HindIII`	1	0	1	Exog
`XRN1_DBM`	1	0	1	Tmub2
`C2-set`	1	0	1	CD33
`Cytochrom_C_2`	1	0	1	Eef1d
`DUF4749`	1	0	1	PDLIM7
`Rv0078B`	1	0	1	PDLIM7
`DUF948`	1	0	1	Ccpg1
`Rubredoxin_2`	1	0	1	Araf
`DNA_pol_phi`	1	0	1	Apbb1
`LCD1`	1	1	0	Thyn1
`Ribophorin_I`	1	0	1	Rpusd1
`ODAPH`	1	1	0	Mcts1
`Cons_hypoth698`	1	0	1	Lamp5
`Asp4`	1	0	1	Lamp5
`Renin_r`	1	0	1	Lamp5
`DUF819`	1	0	1	Lamp5
`bZIP_Maf`	1	0	1	Sh3glb2
`DUF417`	1	0	1	Zdhhc4
`MKRN1_C`	1	0	1	Mkrn1
`zf-RING_10`	1	1	0	Mkrn1
`B56`	1	0	1	Mkrn1
`TPR_17`	1	0	1	Anapc5
`Ig_3`	1	0	1	Lsr
`Thump_like`	1	0	1	Naa10
`Kp4`	1	1	0	Ociad1
`BCLP`	1	1	0	Ociad1
`zf-C2H2_11`	1	1	0	Ociad1
`DUF5931`	1	0	1	Cox18
`MGC-24`	1	0	1	Cadm1
`Mid2`	1	0	1	Cadm1
`DUF4726`	1	0	1	Hspbp1
`ROK`	1	1	0	Nagk
`DUF6277`	1	0	1	Psap
`Tricorn_PDZ`	1	0	1	Htra2
`DUF6777`	1	0	1	Nherf2
`2_5_RNA_ligase2`	1	1	0	Ubc
`TraK`	1	1	0	Ubc
`DUF969`	1	1	0	Ubc
`DMA`	1	1	0	Ubc
`DUF5830`	1	0	1	ZNF286A
`DUF6076`	1	0	1	ZNF286A
`zinc_ribbon_9`	1	0	1	ZNF286A
`zf-LYAR`	1	0	1	ZNF286A
`SOXp`	1	0	1	MT1G
`DUF2813`	1	0	1	Pak1
`Ubiquitin_5`	1	0	1	Kxd1
`Ubiquitin_4`	1	0	1	Kxd1
`Ribosomal_L40e`	1	0	1	Kxd1
`Rad60-SLD`	1	0	1	Kxd1
`DUF2604`	1	0	1	Kxd1
`Ubiquitin_2`	1	0	1	Kxd1
`TBK1_ULD`	1	0	1	Kxd1
`Sde2_N_Ubi`	1	0	1	Kxd1
`Rad60-SLD_2`	1	0	1	Kxd1
`Syntaxin_2`	1	0	1	Stx5
`DUF4795`	1	0	1	Stx5
`WASH_WAHD`	1	0	1	Stx5
`QWRF`	1	0	1	Stx5
`Prominin`	1	0	1	Stx5
`Cep3`	1	0	1	Stx5
`Nuf2_DHR10-like`	1	0	1	Stx5
`DUF5898`	1	0	1	Stk16
`SARAF`	1	0	1	Stk16
`HHH`	1	0	1	Polb
`HHH_5`	1	0	1	Polb
`IMS_HHH`	1	0	1	Polb
`WSN`	1	0	1	Polb
`DUF3701`	1	0	1	Polb
`Cdd1`	1	0	1	Polb
`HHH_8`	1	0	1	Polb
`HHH_3`	1	0	1	Polb
`HHH_2`	1	0	1	Polb
`DNA_pol_lambd_f`	1	0	1	Polb
`MMM1`	1	0	1	Meaf6
`RuvB_N`	1	0	1	Ak6
`TsaE`	1	0	1	Ak6
`Mg_chelatase`	1	0	1	Ak6
`IstB_IS21`	1	0	1	Ak6
`ATPase`	1	0	1	Ak6
`PduV-EutP`	1	0	1	Ak6
`AAA_3`	1	0	1	Ak6
`AAA_30`	1	0	1	Ak6
`Thymidylate_kin`	1	0	1	Ak6
`NACHT`	1	0	1	Ak6
`KTI12`	1	0	1	Ak6
`NTPase_1`	1	0	1	Ak6
`AAA_14`	1	0	1	Ak6
`AAA_24`	1	0	1	Ak6
`AAA_5`	1	0	1	Ak6
`CBF`	1	0	1	Nap1l1
`GEN1_C`	1	0	1	Nap1l1
`YopD`	1	1	0	Fam107a
`Cas9_C`	1	0	1	Aspscr1
`CtnDOT_TraJ`	1	0	1	Tpd52l1
`Fib_alpha`	1	0	1	Tpd52l1
`WEMBL`	1	0	1	Tpd52l1
`PDZ`	1	0	1	Trappc4
`TUTase`	1	0	1	Mtpap
`AltA1`	1	0	1	Gnptg
`Bac_chlorC`	1	0	1	Pcbp2
`MAJIN`	1	0	1	Srsf5
`PRP38_assoc`	1	0	1	Srsf5
`Ephrin`	1	0	1	OLFM2
`PepSY_2`	1	0	1	OLFM2
`RR_TM4-6`	1	0	1	Zcchc17
`Nup35_RRM_2`	1	0	1	HNRNPA2B1
`Peptidase_M99_m`	1	0	1	Micu1
`R3H`	1	0	1	Arpp21
`NUDIX_5`	1	1	0	Hpf1
`Mo25`	1	0	1	Chmp2a
`DARPP-32`	1	0	1	Mterf4
`PsbY`	1	0	1	Gabrd
`AAR2`	1	0	1	Use1
`CHDNT`	1	0	1	Hmgb3
`RNA_pol_3_Rpc31`	1	0	1	Hmgb3
`CLEC16A_C`	1	0	1	Hmgb3
`UPF0449`	1	0	1	Bloc1s2
`Sec1`	1	0	1	Bloc1s2
`DivIC`	1	0	1	Bloc1s2
`ZapA`	1	0	1	Bloc1s2
`CENP-Q`	1	0	1	Bloc1s2
`CCD97-like_C`	1	0	1	Ttc33
`DUF2112`	1	0	1	Ttc33
`zf-RanBP`	1	0	1	Ttc33

Domain Variant Genes (617 genes, multiple architectures after QC)

All domain variant genes retaining multiple domain architectures after clan collapse and density artifact removal. Use the search box to filter by gene ID, name, function, or domain.

Showing 617 of 617 genes

Gene	Name	Function	Isoforms	Arch	Tier	CPM	Source	Core Domains
`ENSMUSG00000021288`	Klc1	Kinesin light chain 1	5	5	1	372.7	Mixed	ANAPC3, COG2, DUF4919, DUF627, GHL5, GlfT2_domain3, JIP_LZII, PPR, PPR_2, RNPP_C, RPN7, SHNi-TPR, SOGA, TPR_1, TPR_10, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_4, TPR_7, TPR_8, TPR_MalT, YabA
`ENSMUSG00000021759`	Plpp1	Phospholipid phosphatase 1	4	4	1	39.7	Novel	PAP2, PAP2_C
`ENSMUSG00000000827`	Tpd52l2	Tumor protein D54	6	4	1	23.8	Mixed	HIP1_clath_bdg, TPD52, VASP_tetra
`ENSMUSG00000022332`	Khdrbs3	KH domain-containing, RNA-binding, signal transduction-associated protein 3	5	4	1	105.7	Mixed	FSA_C, KH_1, Qua1, STAR_dimer, Sam68-YY
`ENSMUSG00000008348`	Ubc	Polyubiquitin-C	6	4	1	174.5	Novel	Big_7, CFAP298, CorA, Crinkler, DUF1942, DUF2604, DUF3861, DUF5450, DUF6393, Dsc3_N, FlgD_ig, HemeBinding_Shp, LBP_C, ORF11CD3, Phage_sheath_1N, Plexin_RBD, ProRS-C_2, Pullulanase_N2, Rad60-SLD, Rad60-SLD_2, Staphylokinase, TBK1_ULD, TmoB, UDP-g_GGTase, Ubiquitin_2, Ubiquitin_4, Ubiquitin_5, ubiquitin
`ENSMUSG00000023973`	Cnpy3	Protein canopy homolog 3	4	4	1	108.4	Mixed	DUF3456, SapB_1, TBK1_CCD1
`ENSMUSG00000063406`	Tmed5	Transmembrane emp24 domain-containing protein 5	3	3	1	15.9	Ref	EMP24_GP25L
`ENSMUSG00000028478`	CLTA	Clathrin light chain A	4	3	1	555.1	Ref	CENP-Q, Clathrin_lg_ch, ORC3_N
`ENSMUSG00000004207`	Psap	Prosaposin	4	3	1	647.7	Mixed	CYSTM, DUF3456, DUF6487, SapA, SapB_1, SapB_2
`ENSMUSG00000025142`	Aspscr1	Tether containing UBX domain for GLUT4	3	3	1	50.2	Ref	QWRF, RBD, TUG-UBL1, UBX
`ENSMUSG00000022010`	TSC22D1	TSC22 domain family protein 1	5	3	1	700.5	Mixed	Chibby, DUF5660, DUF6262, DivIC, Fzo_mitofusin, K-box, SOGA, TSC22, YabA, ZapB, bZIP_1, bZIP_2
`ENSMUSG00000024462`	Gabbr1	Gamma-aminobutyric acid type B receptor subunit 1	3	3	1	44.9	Ref	7tm_3, ANF_receptor, CCDC-167, COX1, DUF3995, DUF6377, Kei1, NEMP, PKcGMP_CC, Peripla_BP_6, Sushi, UPF0242
`ENSMUSG00000033379`	Atp6v0b	V-type proton ATPase 21 kDa proteolipid subunit c''	3	3	1	1,379.9	Mixed	ATP-synt_C, G0-G1_switch_2, PIRT
`ENSMUSG00000003872`	Lin7b	Protein lin-7 homolog B	3	3	1	185.0	Novel	DUF2057, L27, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000035086`	Becn1	Beclin-1	3	3	1	21.1	Mixed	ALMT, APG6, APG6_N, BH3, DASH_Hsk3, DUF3450, DUF4164, DUF4200, Exonuc_VII_L, FapA, GAS, HAUS-augmin3, MT, NAPRTase_C, PI_PP_I, Suppressor_APC, VPS38, V_ATPase_I, YabA
`ENSMUSG00000036503`	Rnf13	E3 ubiquitin-protein ligase RNF13	3	3	1	18.5	Ref	Baculo_11_kDa, FeoB_associated, LapA_dom, Ninjurin, PA, zf-C3HC4, zf-C3HC4_2, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000028861`	Mrps15	Small ribosomal subunit protein uS15m	3	3	1	137.5	Novel	DUF272, DUF4140, DUF724, MIX23, PIN_4, Ribosomal_S15
`ENSMUSG00000060373`	Hnrnpc	Heterogeneous nuclear ribonucleoproteins C1/C2	5	3	1	142.8	Ref	RRM_1, SKA1, Saf4_Yju2, XhlA
`ENSMUSG00000031996`	Aplp2	Amyloid beta precursor like protein 2	3	3	1	185.0	Mixed	APP_Cu_bd, APP_E2, APP_N, APP_amyloid, Activator-TraM, RapH_N, VHL_C
`ENSMUSG00000027566`	Psma7	Proteasome subunit alpha type-7	3	3	1	639.7	Mixed	Proteasome, Proteasome_A_N
`ENSMUSG00000020458`	Rtn4	Reticulon-4	3	3	1	467.9	Novel	DUF4736, DUF639, Reticulon
`ENSMUSG00000044477`	Zfand3	AN1-type zinc finger protein 3	3	3	1	18.5	Ref	zf-A20, zf-AN1
`ENSMUSG00000045251`	ZNF688	Zinc finger protein 688	4	3	1	23.8	Mixed	FYVE_2, KRAB, OrfB_Zn_ribbon, TFIIS_C, Vps36-NZF-N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-ISL3, zf-LYAR, zf-met, zf_UBZ, zinc_ribbon_9
`ENSMUSG00000035642`	Aamdc	Mth938 domain-containing protein	3	3	1	87.2	Ref	DUF498, Indigoidine_A, UPF0180
`ENSMUSG00000063236`	—	UPF0488 protein C8orf33 homolog	3	3	1	68.7	Ref	CtsR_C, DUF4615, PDE4_UCR
`ENSMUSG00000024914`	Drap1	Dr1-associated corepressor	3	3	1	177.1	Ref	CBFD_NFYB_HMF, Histone, PPP4R2, TAF4
`ENSMUSG00000040242`	Fgfr1op2	FGFR1 oncogene partner 2 homolog	3	3	1	68.7	Ref	Anthrax_toxA, DASH_Dam1, DUF16, Exonuc_VII_L, FUSC, FapA, Glyoxalase_8, Mgr1, NYD-SP28_assoc, PCRF, SIKE, TSC22, Tho2
`ENSMUSG00000024381`	Bin1	Myc box-dependent-interacting protein 1	3	3	1	95.2	Mixed	BAR, BLOC1_2, Baculo_PEP_C, DUF5667, DUF948, Laminin_II, PTS_IIA, SH3_1, SH3_2, SH3_9, THP2, YtxH
`ENSMUSG00000031570`	Plpp5	Phospholipid phosphatase 5	4	3	1	26.4	Ref	Anthrone_oxy, CoxIIa, PAP2
`ENSMUSG00000034681`	RNPS1	RNA-binding protein with serine-rich domain 1	6	3	1	113.7	Mixed	Macoilin, Nup35_RRM_2, PI3K_1B_p101, RRM_1, RRM_3, RRM_5, RRM_7, RRM_occluded
`ENSMUSG00000031342`	Gpm6b	Neuronal membrane glycoprotein M6-b	3	3	1	37.0	Ref	DUF2755, DUF373, Myelin_PLP, PspB
`ENSMUSG00000006575`	Rundc3a	RUN domain-containing protein 3A	4	3	1	150.7	Ref	DUF3450, DUF5595, Phage_GP20, RUN, UPF0242, WT1
`ENSMUSG00000030330`	Ing4	Inhibitor of growth protein 4	3	3	1	150.7	Ref	DUF6482, ING, LMBR1, PHD, SOGA, zf-HC5HC2H
`ENSMUSG00000021040`	Slirp	SRA stem-loop-interacting RNA-binding protein, mitochondrial	3	3	1	23.8	Ref	Nup35_RRM, Nup35_RRM_2, PB1, RRM_1
`ENSMUSG00000022044`	Stmn4	Stathmin-4	4	3	1	703.2	Ref	ATG14, DHR-2_Lobe_C, EF-hand_14, Exonuc_VII_L, FUT8_N_cat, Pex19, Ribonuc_2-5A, Stathmin, UPF0242
`ENSMUSG00000030649`	Anapc15	Anaphase-promoting complex subunit 15	3	3	1	18.5	Mixed	ANAPC15, Astro_capsid_p, BUD22, CDC45, DUF2457, Med8, Nop14, Pox_Ag35, Presenilin, RNA_pol_Rpc4, TFB6, V_ATPase_I
`ENSMUSG00000019194`	Scn1b	Sodium channel regulatory subunit beta-1	3	3	1	142.8	Mixed	Ig_3, V-set
`ENSMUSG00000118664`	Tusc3	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3	3	3	1	63.4	Mixed	DUF4131, Gram_pos_anchor, OST3_OST6, Thioredoxin
`ENSMUSG00000000355`	Mcts1	Malignant T-cell-amplified sequence 1	5	3	1	60.8	Mixed	DUF1947, Methyltr_RsmF_N, PUA, Pre-PUA
`ENSMUSG00000027270`	Lamp5	Lysosome-associated membrane glycoprotein 5	3	3	1	512.8	Mixed	Chordopox_A13L, DUF4083, Lamp, VAS1_LD
`ENSMUSG00000026860`	Sh3glb2	Endophilin-B2	7	3	1	261.7	Mixed	BAR, BAR_2, BAR_3, IMD, SH3_1, SH3_2, SH3_9
`ENSMUSG00000029922`	Mkrn1	E3 ubiquitin-protein ligase makorin-1	5	3	1	39.7	Mixed	Torus, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf-CCCH_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1, zf_CCCH_4
`ENSMUSG00000032076`	Cadm1	Cell adhesion molecule 1	4	3	1	5.3	Mixed	Adeno_E3_CR1, Adhes-Ig_like, C1-set, C2-set, C2-set_2, DUF3439, I-set, Ig_2, Ig_3, Ig_4, Ig_C17orf99, Izumo-Ig, SSP160, Syndecan, V-set, ig
`ENSMUSG00000068329`	Htra2	Serine protease HTRA2, mitochondrial	3	3	1	47.6	Ref	GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Peptidase_S32, Peptidase_S46, Trypsin, Trypsin_2
`ENSMUSG00000002504`	Nherf2	Na(+)/H(+) exchange regulatory cofactor NHE-RF2	4	3	1	42.3	Ref	DUF1450, DUF5994, DUF6288, EBP50_C, GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000047342`	ZNF286A	Zinc finger protein 286A	3	3	1	15.9	Ref	C1_4, DNA_RNApol_7kD, DZR, HVO_2753_ZBP, HalOD2, KRAB, LIM, OSTMP1, TFIIS_C, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-met, zf-trcl, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000030774`	Pak1	Serine/threonine-protein kinase PAK 1	4	3	1	89.9	Novel	ABC1, APH, DSHCT, DUF3741, Kdo, Kinase-like, PBD, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal
`ENSMUSG00000055553`	Kxd1	KxDL motif-containing protein 1	4	3	1	116.3	Mixed	KxDL, TolA_bind_tri
`ENSMUSG00000022884`	EIF4A2	Eukaryotic initiation factor 4A-II	4	3	1	967.5	Ref	AAA_19, AAA_22, AAA_30, CMS1, DEAD, Fanconi_A, Flavi_DEAD, Helicase_C, Helicase_RecD, ResIII, UvrD-helicase, tRNA-synt_1b
`ENSMUSG00000028772`	Zcchc17	Zinc finger CCHC domain-containing protein 17	4	3	1	15.9	Novel	CDC45, Coilin_N, CpXC, DUF3827, DUF4604, Hid1, Menin, S1, SR-25, zf-CCHC
`ENSMUSG00000038005`	Hpf1	Histone PARylation factor 1	5	3	1	163.9	Mixed	DUF2228, DUF6027
`ENSMUSG00000057506`	Bloc1s2	Biogenesis of lysosome-related organelles complex 1 subunit 2	3	3	1	182.4	Mixed	BLOC1_2, DUF948, Fib_alpha, HEF_HK, NPV_P10, ParB, XhlA
`ENSMUSG00000063179`	Pstk	L-seryl-tRNA(Sec) kinase	3	3	4	29.1	Novel	KTI12, LMBR1
`ENSMUSG00000071454`	Dtnb	Dystrobrevin beta	3	3	4	7.9	Mixed	CRISPR_Cse2, EF-hand_2, EF-hand_3, MerR-DNA-bind
`ENSMUSG00000062753`	SMIM29	Small integral membrane protein 29	3	3	4	39.7	Ref	DUF3267, DUF3619, DUF4245, Hol_Tox
`ENSMUSG00000000296`	Tpd52l1	Tumor protein D53	3	3	4	21.1	Mixed	Cnn_1N, CorA, DUF1818, DUF3458_C, DUF745, NUDE_C, TMEM247, TPD52, Uds1
`ENSMUSG00000015217`	Hmgb3	High mobility group protein B3	3	3	4	42.3	Mixed	HMG_box, HMG_box_2, HMG_box_5, Ribosomal_60s, YABBY
`ENSMUSG00000053799`	Exoc6	Exocyst complex component 6	2	2	1	5.3	Ref	AKAP7_NLS, COG5, DUF5856, Sec15
`ENSMUSG00000001018`	Snapin	SNARE-associated protein Snapin	2	2	1	81.9	Ref	CATRA-C, COG2, CorA, DUF1664, DUF5798, E2F_TDP, NPV_P10, Snapin_Pallidin
`ENSMUSG00000037386`	Rims2	Regulating synaptic membrane exocytosis protein 2	2	2	1	26.4	Ref	C2, PDZ, PDZ_6, SOGA
`ENSMUSG00000048076`	ARF1	ADP-ribosylation factor 1	2	2	1	742.8	Ref	6PF2K, Arf, DO-GTPase2, DUF1806, G-alpha, Gtr1_RagA, MMR_HSR1, Ras, Roc, SRPRB
`ENSMUSG00000030881`	Arfip2	Arfaptin-2	2	2	1	68.7	Ref	Arfaptin, BAR, BAR_2, BAR_3, Blo-t-5, TPR_MLP1_2
`ENSMUSG00000002015`	Bcap31	B-cell receptor-associated protein 31	2	2	1	393.9	Novel	APG6_N, ATG14, Bap31, Bap31_Bap29_C, CENP-H, DUF3498, DUF4140, DUF4337, DUF4407, DUF724, DUF730, DUF948, ERM_C, GAS, KASH_CCD, LMBR1, LMF1, PIG-U, Rx_N, Sec34, TolA_bind_tri, YabA
`ENSMUSG00000034951`	Cog7	Conserved oligomeric Golgi complex subunit 7	2	2	1	26.4	Novel	AbiGi, COG5, COG7, FlgN
`ENSMUSG00000021124`	Vti1b	Vesicle transport through interaction with t-SNAREs homolog 1B	2	2	1	71.4	Ref	4HB_MCP_1, CCDC-167, Cir_Bir_Yir, DASH_Duo1, DUF1759, DUF3376, DUF3958, DUF6245, DUF745, Exonuc_VII_L, Fusion_gly, KxDL, Laminin_II, OmpH, Sec20, Snapin_Pallidin, Syntaxin-6_N, UPF0449, V-SNARE, V-SNARE_C, YqjK
`ENSMUSG00000047547`	Cltb	Clathrin light chain B	2	2	1	256.4	Ref	CENP-Q, Clathrin_lg_ch, DUF6161, GED
`ENSMUSG00000034484`	Snx2	Sorting nexin-2	2	2	1	39.7	Novel	4HB_MCP_1, Allexi_40kDa, Arfaptin, BAR_3_WASP_bdg, BLOC1_2, DUF1870, DUF3775, DUF4618, DUF4795, Dynamin_N, Exonuc_VII_L, FAM92, FapA, Fez1, Fib_alpha, FliD_N, GrpE, HMMR_N, HR1, PX, Prefoldin_2, Sec34, Sorting_nexin, Vps5
`ENSMUSG00000020903`	Stx8	Syntaxin-8	2	2	1	13.2	Ref	DUF1731, DUF2095, Otopetrin, Prominin, SNARE, Sgf11, TFIIS_M, Use1, zf-ANAPC11, zf_C2HC_14
`ENSMUSG00000010110`	Stx5	Syntaxin-5	3	2	1	26.4	Ref	Apolipoprotein, BLOC1_2, DUF16, DUF1664, DUF5063, DUF5082, Fib_alpha, IZUMO, LPP, MCPsignal, NPV_P10, SKA1, SNARE, Spectrin, Syntaxin, Syntaxin-5_N, Troponin, Vac_Fusion
`ENSMUSG00000038000`	Acd	Adrenocortical dysplasia protein	2	2	1	13.2	Novel	OGFr_III, TEBP_beta, TPP1
`ENSMUSG00000031539`	Ap3m2	AP-3 complex subunit mu-2	3	2	1	7.9	Ref	Adap_comp_sub, Clat_adaptor_s, DUF11
`ENSMUSG00000029128`	Rab28	Ras-related protein Rab-28	2	2	1	105.7	Ref	Arf, DLIC, G-alpha, Gtr1_RagA, PduV-EutP, Ras, Roc, WHAMM-JMY_N, XFP_N
`ENSMUSG00000021486`	RAB24	Ras-related protein Rab-24	2	2	1	756.1	Ref	AAA_24, PRELI, Ras, Roc
`ENSMUSG00000034789`	Rab24	Ras-related protein Rab-24	2	2	1	87.2	Novel	Arf, FeoB_N, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, tRNA_anti-codon
`ENSMUSG00000001240`	Ramp2	Receptor activity-modifying protein 2	2	2	1	95.2	Ref	ExbD, RAMP
`ENSMUSG00000020409`	Slu7	Pre-mRNA-splicing factor SLU7	2	2	1	0.0	Novel	Cadherin_tail, DNA_pol_D_N, Rtf2, Slu7, zf-CCHC, zf-CCHC_4
`ENSMUSG00000030058`	Copg1	Coatomer subunit gamma-1	2	2	4	198.3	Ref	Adaptin_N, COP-gamma_platf, Cm_res_leader, Cnd1, Cnd3, Coatomer_g_Cpla, HEAT, HEAT_2, HEAT_EZ, HEAT_PBS, MOR2-PAG1_C, PDS5, UNC45-central
`ENSMUSG00000026797`	STXBP1	Syntaxin-binding protein 1	2	2	4	436.2	Ref	DUF5344, FRB_dom, Sec1, YlbD_coat
`ENSMUSG00000024610`	Cd74	H-2 class II histocompatibility antigen gamma chain	2	2	4	5.3	Ref	DUF2722, DUF6082, MHC2-interact, MHCassoc_trimer, Thyroglobulin_1
`ENSMUSG00000029146`	Snx17	Sorting nexin-17	2	2	4	108.4	Ref	DUF2777, FERM_M, Nod1, PX, RA, Ras_bdg_2, SNX17_FERM_C
`ENSMUSG00000020232`	Hmg20b	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related	3	2	1	34.4	Ref	AAA_11, CTD9, DASH_Duo1, DUF3496, DUF4763, HMG_box, HMG_box_2, PI3K_P85_iSH2, TetR_C_15
`ENSMUSG00000060073`	Psma3	Proteasome subunit alpha type-3	4	2	1	623.9	Ref	Arm-DNA-bind_3, Proteasome, Proteasome_A_N
`ENSMUSG00000060450`	Rnf14	E3 ubiquitin-protein ligase RNF14	3	2	1	89.9	Ref	IBR, RWD, TBPIP, zf-C3HC4, zf-RING_11, zf-RING_2, zf-RING_5
`ENSMUSG00000021792`	Prxl2a	Peroxiredoxin-like 2A	3	2	1	179.8	Novel	AhpC-TSA, AhpC-TSA_2, DUF2231, Redoxin, UCR_Fe-S_N
`ENSMUSG00000040276`	Pacsin1	Protein kinase C and casein kinase substrate in neurons protein 1	3	2	1	13.2	Ref	DUF2605, FCH, RPS31, SH3_1, SH3_2, SH3_9, Terpene_syn_C_2
`ENSMUSG00000035949`	Fbxw2	F-box/WD repeat-containing protein 2	3	2	1	47.6	Ref	ANAPC4_WD40, DUF5570, F-box, F-box-like, NBCH_WD40, WD40
`ENSMUSG00000037706`	Cd81	CD81 antigen	2	2	1	1,485.7	Novel	DUF6768, Tetraspanin
`ENSMUSG00000019188`	Hm13	Signal peptide peptidase	4	2	1	81.9	Mixed	DUF4345, Peptidase_A22B, Presenilin, SPP
`ENSMUSG00000029755`	Dlx5	Homeobox protein DLX-5	2	2	1	34.4	Ref	Cauli_DNA-bind, DLL_N, Homeobox_KN, Homeodomain, YdaS_antitoxin
`ENSMUSG00000057236`	RBBP4	Histone-binding protein RBBP4	7	2	1	15.9	Mixed	CAF1C_H4-bd, Capsid_NCLDV, WD40
`ENSMUSG00000079494`	Nat8f5	Probable N-acetyltransferase family 8 member 5	2	2	1	0.0	Novel	Acetyltransf_1, Acetyltransf_10, Acetyltransf_15, Acetyltransf_3, Acetyltransf_4, Acetyltransf_7, Acetyltransf_8, Acetyltransf_9, Acetyltransf_CG, DUF4131, FR47
`ENSMUSG00000004268`	Emg1	Ribosomal RNA small subunit methyltransferase NEP1	2	2	1	108.4	Novel	CHMI, EMG1
`ENSMUSG00000061032`	Rrp1	Ribosomal RNA processing protein 1 homolog A	2	2	1	510.2	Novel	DNA_pol_phi, DRIM, DUF1981, DUF4391, DUF4776, MMS19_N, Nop52, PFam54_60, Tn7_Tnp_TnsA_C
`ENSMUSG00000041765`	Ubac2	Ubiquitin-associated domain-containing protein 2	2	2	1	39.7	Novel	FeoA, HA, Rhomboid, UBA
`ENSMUSG00000025967`	Eef1b	Elongation factor 1-beta	2	2	1	71.4	Ref	EF-1_beta_acid, EF1_GNE, GST_C_3, GST_C_6
`ENSMUSG00000029657`	Hsph1	Heat shock protein 105 kDa	2	2	1	208.8	Ref	CCDC90-like, CHAD, F-box-like_2, FGGY_C, FtsA, HSP70, MreB_Mbl, Spectrin
`ENSMUSG00000035776`	Cd99l2	CD99 antigen-like protein 2	2	2	1	2.6	Ref	ACT_7, CD99L2, Podoplanin, Ribosomal_L23eN
`ENSMUSG00000030226`	LMO3	LIM domain only protein 3	2	2	1	58.2	Ref	LIM, Siva
`ENSMUSG00000027937`	Jtb	Protein JTB	2	2	1	148.0	Novel	DUF1505, DUF3703, JTB
`ENSMUSG00000033526`	Ppip5k1	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1	2	2	1	13.2	Ref	PPIP5K2_N, RimK
`ENSMUSG00000031622`	Sin3b	Paired amphipathic helix protein Sin3b	2	2	1	121.6	Ref	DUF5077, PAH, Sin3_corepress, Sin3a_C
`ENSMUSG00000031156`	Slc35a2	UDP-galactose translocator	2	2	1	18.5	Ref	EamA, Nuc_sug_transp, PUNUT, TPT, TraL, UAA
`ENSMUSG00000001105`	Ift20	Intraflagellar transport protein 20 homolog	2	2	1	396.5	Ref	4HB_MCP_1, DUF3135, HRDC, IFT20, Nnf1, TMPIT, YabA
`ENSMUSG00000006288`	Ttc5	Tetratricopeptide repeat protein 5	2	2	1	95.2	Ref	ANAPC3, Abi_C, CK2S, DUF6584, MAS20, RPN7, TPR-S, TPR_1, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_8, TPR_9, TTC5_OB
`ENSMUSG00000041112`	Elmo1	Engulfment and cell motility protein 1	2	2	1	169.2	Ref	ELMO_ARM, ELMO_CED12, FERM_N, Imm42, Nup96, PH_12, ZFYVE21_C
`ENSMUSG00000021156`	Zmynd11	Zinc finger MYND domain-containing protein 11	2	2	1	5.3	Ref	Bacillus_HBL, Bromodomain, CCDC-167, Cep57_MT_bd, DUF1866, DUF5740, DUF6626, HSP70, PSD4, PWWP, Spore_III_AB, TPX2, zf-MYND
`ENSMUSG00000026888`	Grb14	Growth factor receptor-bound protein 14	2	2	1	81.9	Novel	BPS, PH, PH_20, RA, SH2
`ENSMUSG00000027012`	Dync1i2	Cytoplasmic dynein 1 intermediate chain 2	2	2	1	10.6	Ref	ANAPC4_WD40, DUF6465, Dynein_IC2, FYDLN_acid, SOBP, WD40
`ENSMUSG00000020912`	Krt12	Keratin, type I cytoskeletal 12	2	2	1	47.6	Novel	ADIP, APC_N_CC, ATG16, Bacillus_HBL, COG2, DUF1664, DUF4407, DivIC, ERM_helical, Filament, LPP, Mod_r, SKA1, Sec2p, Sec34, Tweety, Uso1_p115_C, V_ATPase_I, YabA, zf-C4H2
`ENSMUSG00000004187`	Kifc2	Kinesin-like protein KIFC2	2	2	1	18.5	Novel	Baculo_PEP_C, DUF4407, DUF6779, Fib_alpha, Kinesin, Microtub_bd, Rubis-subs-bind, TACC_C, THOC7
`ENSMUSG00000027466`	Rbck1	RanBP-type and C3HC4-type zinc finger-containing protein 1	2	2	1	21.1	Novel	IBR, Rad50_zn_hook, ubiquitin, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-RanBP
`ENSMUSG00000045867`	Cradd	Death domain-containing protein CRADD	2	2	1	13.2	Ref	CARD, Death, Gam, TadE, Tmemb_18A
`ENSMUSG00000025825`	Iscu	Iron-sulfur cluster assembly enzyme ISCU	2	2	1	227.3	Ref	Jag_N, NifU_N
`ENSMUSG00000037966`	Ninj1	Ninjurin-1	2	2	1	47.6	Ref	DUF4675, Ninjurin
`ENSMUSG00000007570`	FANCE	Fanconi anemia group E protein	2	2	1	5.3	Ref	Bystin, FA_FANCE
`ENSMUSG00000020946`	Gosr2	Golgi SNAP receptor complex member 2	2	2	1	23.8	Ref	CLP_protease, DUF6730, EF-G-binding_N, Sec20, V-SNARE, V-SNARE_C
`ENSMUSG00000029276`	Glmn	Glomulin	2	2	1	13.2	Ref	DUF3911, Kinetochor_Ybp2
`ENSMUSG00000078919`	Dpm1	Dolichol-phosphate mannosyltransferase subunit 1	2	2	1	34.4	Ref	Glyco_tranf_2_2, Glyco_tranf_2_3, Glyco_tranf_2_4, Glyco_transf_21, Glycos_transf_2, MapZ_C2
`ENSMUSG00000022771`	Ppil2	RING-type E3 ubiquitin-protein ligase PPIL2	3	2	1	13.2	Novel	Pro_isomerase, Rtf2, SUB1_ProdP9, U-box, zf-NOSIP
`ENSMUSG00000063457`	RPS15	Small ribosomal subunit protein uS19	2	2	1	1,229.2	Ref	LCAT, MAP65_ASE1, Prok_Ub, Ribosomal_S19
`ENSMUSG00000044533`	Rps2	Small ribosomal subunit protein uS5	3	2	1	84.6	Novel	Ribosomal_S5, Ribosomal_S5_C
`ENSMUSG00000045128`	Rpl18a	Large ribosomal subunit protein eL20	4	2	1	26.4	Ref	Ribosomal_L18A
`ENSMUSG00000030621`	Me3	NADP-dependent malic enzyme, mitochondrial	3	2	1	10.6	Novel	malic
`ENSMUSG00000053768`	Chchd3	MICOS complex subunit Mic19	2	2	1	68.7	Novel	CHCH, DUF1690, DUF885, ECH_2, Fes1, MIC19_MIC25, NDUF_B7
`ENSMUSG00000031887`	Tradd	Tumor necrosis factor receptor type 1-associated DEATH domain protein	2	2	1	0.0	Novel	D_CNTX, Death, TPR_14, TRADD_N
`ENSMUSG00000029199`	Lias	Lipoyl synthase, mitochondrial	2	2	1	47.6	Ref	Glyco_transf_4, LIAS_N, ROS_MUCR, Radical_SAM, SVWC
`ENSMUSG00000014195`	Dnajc7	DnaJ homolog subfamily C member 7	3	2	1	259.1	Ref	ANAPC3, Alkyl_sulf_dimr, BTAD, DnaJ, MIT, NFACT-R_1, RPN7, RPT, Rapsyn_N, SHNi-TPR, Sel1, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_3, TPR_4, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000035297`	Cops4	COP9 signalosome complex subunit 4	3	2	1	190.3	Novel	CSN4_RPN5_eIF3a, DinB, PCI, RPN7, TPR_12, TPR_7, TPR_8
`ENSMUSG00000026632`	Tatdn3	Putative deoxyribonuclease TATDN3	2	2	1	31.7	Ref	MogR_DNAbind, TatD_DNase
`ENSMUSG00000035849`	Krt222	Keratin-like protein KRT222	2	2	1	29.1	Ref	Allexi_40kDa, BCAS2, Baculo_PEP_C, Band_7, DUF1664, DUF2381, DUF4140, DUF5830, DUF6594, DUF745, FUSC, Fez1, Filament, FlaC_arch, KMP11, Macoilin, TPR_MLP1_2, V_ATPase_I
`ENSMUSG00000059361`	Nrsn2	Neurensin-2	2	2	1	243.2	Novel	DUF2207, Neurensin, Phage_holin_3_6
`ENSMUSG00000078584`	—	Uncharacterized protein C1orf50 homolog	2	2	1	5.3	Ref	DUF2452, DUF3975, DUF5993, Gallidermin
`ENSMUSG00000021660`	Btf3	Transcription factor BTF3	2	2	1	605.4	Ref	LolA, Myco_19_kDa, NAC
`ENSMUSG00000022415`	Syngr1	Synaptogyrin-1	2	2	1	264.4	Ref	Col_cuticle_N, DUF4064, DUF805, Fig1, MARVEL, Ost5
`ENSMUSG00000006058`	Snf8	Vacuolar-sorting protein SNF8	2	2	1	145.4	Ref	DUF4896, EAP30, EutK_C, Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C, ZapD
`ENSMUSG00000062006`	Rpl34	Large ribosomal subunit protein eL34	3	2	1	50.2	Ref	Ribosomal_L34e
`ENSMUSG00000094936`	Rbm4b	RNA-binding protein 4B	2	2	1	10.6	Ref	Calcipressin, CompInhib_SCIN, DbpA, Nup35_RRM_2, RNA_bind, RRM_1, RRM_5, RRM_7, RRM_occluded, RSD-2, zf-CCHC, zf-CCHC_2, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000023021`	Cers5	Ceramide synthase 5	2	2	1	13.2	Ref	DUF1003, Homeodomain, TRAM_LAG1_CLN8
`ENSMUSG00000022340`	Sybu	Syntabulin	3	2	1	15.9	Novel	CALCOCO1, CC2-LZ, DUF16, DUF1635, DUF745, FPP, FapA, Fez1, HR1, PKcGMP_CC, Sec2p, Sec8_exocyst, Syntaphilin, TMPIT, TPR_MLP1_2, UPF0242, UPF0561
`ENSMUSG00000020925`	Ccdc43	Coiled-coil domain-containing protein 43	2	2	1	37.0	Ref	RepL, SPECT1, TetR_C_28
`ENSMUSG00000022892`	App	Amyloid-beta precursor protein	2	2	1	1,083.9	Ref	APP_Cu_bd, APP_E2, APP_N, APP_amyloid, Activator-TraM, Beta-APP, DUF5033, Dicty_REP, Exonuc_VII_L, Insulin_TMD, Kunitz_BPTI, PI3K_1B_p101, PilJ, SDA1, Spore_III_AB
`ENSMUSG00000021585`	Cast	Calpastatin	2	2	1	0.0	Ref	Calpain_inhib, RsdA_SigD_bd
`ENSMUSG00000027215`	Cd82	CD82 antigen	2	2	1	47.6	Ref	DUF1310, DUF2070, DUF2157, DUF2981, DUF6057, OAD_gamma, Tetraspanin, Tmemb_55A
`ENSMUSG00000031897`	Psmb10	Proteasome subunit beta type-10	3	2	1	174.5	Novel	NAD_Gly3P_dh_C, Pr_beta_C, Proteasome
`ENSMUSG00000037058`	Paip2	Polyadenylate-binding protein-interacting protein 2	3	2	1	367.5	Novel	PAM2
`ENSMUSG00000034403`	Pja1	E3 ubiquitin-protein ligase Praja-1	2	2	1	92.5	Ref	Amidase, FANCL_C, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000068134`	Zfp120	Zinc finger protein 120	2	2	1	2.6	Ref	Baculo_LEF-11, C1_4, Csm1, DUF285, DUF5830, DnaB, GATA, KRAB, NCD1, REV1_C, RNA_POL_M_15KD, SWIB, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zinc_ribbon_15
`ENSMUSG00000027184`	Caprin1	Caprin-1	2	2	1	95.2	Ref	Caprin-1_C, Caprin-1_dimer
`ENSMUSG00000050088`	—	UPF0669 protein C6orf120 homolog	3	2	1	21.1	Ref	Colicin_immun, Tox-MPTase2, UPF0669
`ENSMUSG00000026179`	Pnkd	Probable thioesterase PNKD	3	2	1	145.4	Ref	HAGH_C, Lactamase_B, Lactamase_B_2, Lactamase_B_3
`ENSMUSG00000032251`	Irak1bp1	Interleukin-1 receptor-associated kinase 1-binding protein 1	2	2	1	29.1	Ref	MerB, SIMPL, fvmX5
`ENSMUSG00000030282`	Cmas	N-acylneuraminate cytidylyltransferase	2	2	1	126.9	Novel	CTP_transf_3, DUF1593, DUF2590, Hydrolase_3, NTP_transf_3
`ENSMUSG00000028423`	Nfx1	Transcriptional repressor NF-X1	2	2	1	15.9	Ref	IP_trans, R3H, zf-NF-X1
`ENSMUSG00000021996`	Esd	S-formylglutathione hydrolase	2	2	1	89.9	Ref	AXE1, Abhydrolase_2, Abhydrolase_3, BD-FAE, Chlorophyllase, Chlorophyllase2, Esterase, Esterase_PHB, Hydrolase_4, Lipase_3, Peptidase_S9, Shadoo
`ENSMUSG00000020849`	YWHAE	14-3-3 protein epsilon	2	2	1	198.3	Novel	14-3-3, Orbi_VP5, Ta0938
`ENSMUSG00000037936`	Scarb1	Scavenger receptor class B member 1	2	2	1	34.4	Ref	CD36, PorA, TMEM51
`ENSMUSG00000022234`	Cct5	T-complex protein 1 subunit epsilon	2	2	1	89.9	Novel	Cpn60_TCP1, aRib
`ENSMUSG00000056962`	Jmjd6	Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6	2	2	1	15.9	Novel	AF-4, CCDC106, Cupin_8, IMUP, JmjC, JmjC_2, SR-25
`ENSMUSG00000018572`	Phf23	PHD finger protein 23	3	2	1	23.8	Ref	C1_1, EBV-NA3, PHD, PHD_2, Pox_Ag35, RNA_pol_3_Rpc31, zf-HC5HC2H, zf-RING_9
`ENSMUSG00000024587`	Nars1	Asparagine--tRNA ligase, cytoplasmic	3	2	1	163.9	Ref	Cir_N, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon
`ENSMUSG00000022792`	Yars2	Tyrosine--tRNA ligase, mitochondrial	2	2	1	21.1	Ref	DUF4884, S4, tRNA-synt_1b
`ENSMUSG00000038141`	TMEM181	Transmembrane protein 181	2	2	1	2.6	Ref	Abhydrolase_9_N, DUF6709, MIG-14_Wnt-bd, YoqO
`ENSMUSG00000060475`	Wtap	Pre-mRNA-splicing regulator WTAP	2	2	1	10.6	Ref	ADIP, ALMT, ATG14, DUF2120, DUF6468, DUF6594, SKA2, SPACA9, Wtap
`ENSMUSG00000024012`	Mtch1	Mitochondrial carrier homolog 1	5	2	1	124.2	Ref	Mito_carr, YdjM
`ENSMUSG00000027282`	Mtch2	Mitochondrial carrier homolog 2	3	2	1	301.4	Ref	Mito_carr
`ENSMUSG00000035984`	Nme5	Nucleoside diphosphate kinase homolog 5	2	2	1	39.7	Ref	Dpy-30, NDK
`ENSMUSG00000020372`	GNB2L1	Small ribosomal subunit protein RACK1	2	2	1	121.6	Ref	ANAPC4_WD40, Cytochrom_D1, DUF1416, Ge1_WD40, IKI3, NBCH_WD40, Nup160, PD40, WD40, WD40_like, eIF2A
`ENSMUSG00000029823`	Luc7l2	Putative RNA-binding protein Luc7-like 2	2	2	1	7.9	Ref	DUF948, LUC7, Methyltransf_32
`ENSMUSG00000027680`	Fxr1	RNA-binding protein FXR1	3	2	1	10.6	Ref	Agenet, FXMRP1_C_core, FXR_C1, KH_1, KH_2, KH_4, KH_9, Tudor_2, Tudor_FRX1
`ENSMUSG00000032186`	Tmod2	Tropomodulin-2	2	2	1	29.1	Ref	LRR_6, Peptidase_M3_N, Tropomodulin
`ENSMUSG00000019809`	Pex3	Peroxisomal biogenesis factor 3	2	2	1	21.1	Ref	DUF6672, Peroxin-3
`ENSMUSG00000025980`	Hspd1	60 kDa heat shock protein, mitochondrial	3	2	1	224.7	Mixed	Cpn60_TCP1, EAD9
`ENSMUSG00000028907`	Utp11	Probable U3 small nucleolar RNA-associated protein 11	2	2	1	18.5	Novel	BCL9, SAM_1, SLATT_5, Utp11
`ENSMUSG00000003531`	Dgcr6	Protein DGCR6	3	2	1	142.8	Ref	DGCR6, DUF2458
`ENSMUSG00000060126`	Tpt1	Translationally-controlled tumor protein	3	2	1	230.0	Novel	DUF6613, TCTP
`ENSMUSG00000025616`	Usp16	Ubiquitin carboxyl-terminal hydrolase 16	2	2	1	15.9	Ref	BRCT_assoc, DUF4638, DUF5442, DapB_C, RbpA, UCH, zf-UBP, zinc_ribbon_10, zinc_ribbon_9
`ENSMUSG00000022757`	TFG	Protein TFG	2	2	1	140.1	Novel	FAM181, MucBP, Ndc1_Nup, PB1
`ENSMUSG00000041278`	Ttc1	Tetratricopeptide repeat protein 1	2	2	1	227.3	Ref	MG2, RPN7, SOAR, TPR_1, TPR_11, TPR_12, TPR_14, TPR_15, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9, eIF3_N
`ENSMUSG00000017421`	Znf207	BUB3-interacting and GLEBS motif-containing protein ZNF207	2	2	1	2.6	Ref	SSP160, zf-C2H2, zf-C2H2_4, zf-FCS
`ENSMUSG00000068039`	Tcp1	T-complex protein 1 subunit alpha	3	2	1	60.8	Ref	Cpn60_TCP1
`ENSMUSG00000039533`	Mmd2	Monocyte to macrophage differentiation factor 2	2	2	1	29.1	Novel	DUF2842, Ferlin_C, HlyIII, Neurexophilin
`ENSMUSG00000050043`	Tmx2	Thioredoxin-related transmembrane protein 2	3	2	1	243.2	Mixed	Thioredoxin
`ENSMUSG00000040044`	Orc3	Origin recognition complex subunit 3	2	2	1	18.5	Ref	Consortin_C, Fe_dep_repr_C, ORC3_N, ORC3_ins, ORC_WH_C, TAFH
`ENSMUSG00000006519`	Cyba	Cytochrome b-245 light chain	2	2	1	39.7	Ref	COPI_assoc, Cg6151-P, Cytochrom_B558a, DUF6030, SdpI, TgpA_N
`ENSMUSG00000039058`	Ak5	Adenylate kinase isoenzyme 5	2	2	1	97.8	Novel	AAA, AAA_16, AAA_17, AAA_18, AAA_19, AAA_22, AAA_24, AAA_28, AAA_33, AAA_5, ADK, ATPase, Cytidylate_kin, Cytidylate_kin2, Dpy-30, Hydin_ADK, MRP-S22, NTPase_1, Ploopntkinase3, Rad17, SKI, SRP54, Thymidylate_kin, Zeta_toxin, dNK
`ENSMUSG00000013698`	Pea15	Astrocytic phosphoprotein PEA-15	3	2	1	158.6	Mixed	DED
`ENSMUSG00000061111`	Mcrip1	Mapk-regulated corepressor-interacting protein 1	2	2	1	290.8	Ref	DUF6551, FAM195, LAP2alpha
`ENSMUSG00000030869`	Ndufab1	Acyl carrier protein, mitochondrial	3	2	1	89.9	Ref	PP-binding, PP-binding_2
`ENSMUSG00000021071`	Trim9	E3 ubiquitin-protein ligase TRIM9	3	2	1	47.6	Ref	DUF2203, Prok-RING_4, Trm112p, fn3, zf-B_box, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000029471`	Camkk2	Calcium/calmodulin-dependent protein kinase kinase 2	2	2	1	29.1	Ref	ABC1, APH, DUF4010, HAND, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Seadorna_VP7
`ENSMUSG00000036371`	Serbp1	SERPINE1 mRNA-binding protein 1	4	2	1	108.4	Ref	HABP4_PAI-RBP1, IHABP4_N
`ENSMUSG00000024826`	Dpf2	Zinc finger protein ubi-d4	2	2	1	37.0	Ref	C1_1, DPF1-3_N, Lar_restr_allev, PHD, PHD_2, PHD_4, zf-C2H2, zf-C2H2_4, zf-H2C2_2, zf-PHD-like
`ENSMUSG00000031841`	Cdh13	Cadherin-13	2	2	1	7.9	Novel	BACON_2, Big_9, Cadherin, Cadherin_2, Cadherin_3, Cadherin_4, Cadherin_pro, Cu-binding_MopE, RET_CLD1, RibLong
`ENSMUSG00000006476`	Nsmf	NMDA receptor synaptonuclear signaling and neuronal migration factor	2	2	1	50.2	Ref	DUF4347, IQ
`ENSMUSG00000027942`	—	Protein C1orf43 homolog	3	2	1	13.2	Ref	6PGD, NICE-3
`ENSMUSG00000002289`	Angptl4	Angiopoietin-related protein 4	2	2	1	5.3	Ref	COG5, DUF16, DUF5798, DivIC, Fib_alpha, Fibrinogen_C, Helo_like_N, HrpB7, HrpJ, SlyX, UPF0242, XhlA
`ENSMUSG00000022336`	EIF3E	Eukaryotic translation initiation factor 3 subunit E	2	2	1	256.4	Novel	CSN8_PSD8_EIF3K, HD_assoc, PARP_reg, PCI, SPESP1, VMAP-M1, eIF3_N
`ENSMUSG00000091803`	Cox16	Cytochrome c oxidase assembly protein COX16 homolog, mitochondrial	2	2	1	58.2	Novel	ADAMTS_CR_2, ADAM_CR, COX16, Chitin_synth_1N, LRR_5
`ENSMUSG00000020646`	Mboat2	Membrane-bound glycerophospholipid O-acyltransferase 2	2	2	1	13.2	Ref	MBOAT, Plasmod_dom_1, Rota_NSP4
`ENSMUSG00000056116`	H2-Q10	H-2 class I histocompatibility antigen, Q10 alpha chain	3	2	1	18.5	Mixed	C1-set, C2-set_2, Ig_3, MHC_I, ig
`ENSMUSG00000054309`	Cpsf3	Cleavage and polyadenylation specificity factor subunit 3	2	2	1	13.2	Ref	Beta-Casp, Beta_lactamase3, CPSF73-100_C, DUF1611, Gp58, Lactamase_B, Lactamase_B_2, Lactamase_B_3, Lactamase_B_6, RMMBL
`ENSMUSG00000014856`	Tmem208	Transmembrane protein 208	3	2	1	113.7	Ref	DUF2207, DUF4191, Halogen_Hydrol, TMEM208_SND2
`ENSMUSG00000033760`	Rbm4b	RNA-binding protein 4B	2	2	1	18.5	Ref	Calcipressin, DbpA, RNA_bind, RRM_1, RRM_5, RRM_7, RRM_occluded, zf-CCHC, zf-CCHC_2, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000019467`	Arhgef25	Rho guanine nucleotide exchange factor 25	2	2	1	29.1	Ref	PH, PH_10, RhoGEF, SAP18
`ENSMUSG00000019978`	Epb41l2	Band 4.1-like protein 2	2	2	1	5.3	Ref	4_1_CTD, FA, FERM_C, FERM_M, FERM_N, Gly_transf_sug, SAB
`ENSMUSG00000040712`	Camta2	Calmodulin-binding transcription activator 2	2	2	1	2.6	Novel	4HB_MCP_1, AGRB_N, Ank, Ank_2, Ank_3, Ank_4, Ank_5, CG-1, IQ, RhoGEF67_u2, SesA, SgrT, TIG
`ENSMUSG00000058267`	Mrps14	Small ribosomal subunit protein uS14m	2	2	1	153.3	Ref	PSS, Ribosomal_S14
`ENSMUSG00000074165`	ZNF878	Zinc finger protein 878	2	2	1	18.5	Ref	C1_4, DUF4476, DUF968, KRAB, Malic_M, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-trcl, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000054484`	Tmem62	Transmembrane protein 62	2	2	1	10.6	Ref	Big_13, Big_7, Metallophos, PIG-P, Polyoma_coat2
`ENSMUSG00000028034`	Fubp1	Far upstream element-binding protein 1	2	2	1	39.7	Ref	DUF1897, KH_1, KH_2, KH_4, KH_5, NAD_kinase_C, SH3_12, Secretin_N
`ENSMUSG00000058587`	Tmod3	Tropomodulin-3	2	2	1	15.9	Ref	LRR_6, Tropomodulin, Usher
`ENSMUSG00000030663`	Smap	Small acidic protein	3	2	1	428.3	Mixed	SMAP
`ENSMUSG00000025290`	RPS24	Small ribosomal subunit protein eS24	3	2	1	1,023.0	Ref	Ribosomal_S24e
`ENSMUSG00000042305`	Tmem183	Transmembrane protein 183	2	2	1	13.2	Ref	DUF6652, F-box-like
`ENSMUSG00000029610`	Aimp2	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2	2	2	1	163.9	Ref	AIMP2_LysRS_bd, GST_C, GST_C_2, GST_C_3, GST_C_6, GTP-bdg_M, Thioredoxin_16
`ENSMUSG00000019139`	Isyna1	Inositol-3-phosphate synthase 1	2	2	1	44.9	Ref	Inos-1-P_synth, NAD_binding_5, UTP15_C
`ENSMUSG00000021546`	HNRNPK	Heterogeneous nuclear ribonucleoprotein K	5	2	1	105.7	Mixed	DJ-1_PfpI, DUF719, KH_1, KH_2, KH_4, KH_5, MOEP19, ROKNT
`ENSMUSG00000061559`	Skic8	Superkiller complex protein 8	2	2	1	145.4	Ref	ANAPC4_WD40, Cytochrom_D1, DUF2415, GSDH, Ge1_WD40, NBCH_WD40, Nse4-Nse3_bdg, Nucleoporin_N, Nup160, PD40, WD40, WD40_like, eIF2A
`ENSMUSG00000020451`	Limk2	LIM domain kinase 2	2	2	1	0.0	Ref	ABC1, APH, DUF6510, Haspin_kinase, Kdo, LIM, PDZ, PDZ_2, PDZ_6, PK_Tyr_Ser-Thr, Peptidase_M50, Pkinase, zf-RRN7
`ENSMUSG00000029713`	GNB2	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	3	2	1	267.0	Ref	ANAPC4_WD40, Cytochrom_D1, Ge1_WD40, NBCH_WD40, WD40, WD40_like
`ENSMUSG00000024299`	Adamts10	A disintegrin and metalloproteinase with thrombospondin motifs 10	2	2	1	2.6	Ref	ADAMTS_CR_3, ADAMTS_spacer1, CCER1, DAGK_cat, P53_TAD, PLAC, Pep_M12B_propep, TSP1_ADAMTS, TSP1_CCN, TSP1_spondin, TSP_1, zf-RING_5
`ENSMUSG00000005873`	Reep5	Receptor expression-enhancing protein 5	2	2	1	222.1	Novel	Phage_holin_3_6, TB2_DP1_HVA22
`ENSMUSG00000121778`	Ube2v1	Ubiquitin-conjugating enzyme E2 variant 1	2	2	1	589.5	Ref	Lipoprotein_21, UQ_con
`ENSMUSG00000025647`	Shisa5	Protein shisa-5	4	2	1	34.4	Novel	MLANA, Shisa
`ENSMUSG00000023345`	Poc1a	POC1 centriolar protein homolog A	3	2	1	13.2	Mixed	ANAPC4_WD40, NBCH_WD40, WD40
`ENSMUSG00000028447`	Dctn3	Dynactin subunit 3	2	2	1	23.8	Ref	DUF4011, Dynactin_p22, Pox_A_type_inc, Spidroin_MaSp
`ENSMUSG00000026857`	Ntmt1	N-terminal Xaa-Pro-Lys N-methyltransferase 1	2	2	1	108.4	Ref	Bin3, CTD4, Methyltransf_11, Methyltransf_12, Methyltransf_2, Methyltransf_23, Methyltransf_25, Methyltransf_29, Methyltransf_31, Methyltransf_PK, Ubie_methyltran
`ENSMUSG00000020817`	Rabep1	Rab GTPase-binding effector protein 1	2	2	1	10.6	Ref	CALCOCO1, CCDC90-like, Cep57_MT_bd, DUF1664, DUF2802, DUF4407, DUF5955, DUF745, Fez1, Fib_alpha, Fib_succ_major, Filament, HAUS6_N, HIP1_clath_bdg, Rab5-bind, Rabaptin, SpoOE-like, TruB_C_2, UPF0242
`ENSMUSG00000027582`	Zgpat	Zinc finger CCCH-type with G patch domain-containing protein	2	2	1	13.2	Ref	ABC_tran_CTD, Agenet, Bacillus_HBL, CCDC-167, G-patch, Ribosomal_L29, zf-CCCH, zf-CCCH_2, zf-CCCH_4, zf_CCCH_4
`ENSMUSG00000028218`	Cibar1	CBY1-interacting BAR domain-containing protein 1	2	2	1	119.0	Novel	DUF6447, FAM92, FapA, P4Ha_N, Syntaxin-6_N, Vps5
`ENSMUSG00000032046`	Abhd12	Lysophosphatidylserine lipase ABHD12	3	2	1	208.8	Ref	Abhydrolase_1, Abhydrolase_11, Abhydrolase_3, Abhydrolase_6, Hydrolase_4, Peptidase_S15, Say1_Mug180, Thioesterase
`ENSMUSG00000074247`	Dda1	DET1- and DDB1-associated protein 1	2	2	1	37.0	Ref	DDA1, DUF3138, Lgl_C
`ENSMUSG00000121485`	Gpr137b	Integral membrane protein GPR137B	2	2	1	21.1	Ref	DUF4059, Lycopene_cyc, SirB
`ENSMUSG00000051355`	Commd1	COMM domain-containing protein 1	2	2	1	21.1	Novel	COMMD1_N, COMM_domain, DUF4476, SAM_KSR1
`ENSMUSG00000016664`	Pacsin2	Protein kinase C and casein kinase substrate in neurons protein 2	2	2	1	2.6	Ref	ATG17_like, FCH, NPF, SH3BP5, SH3_1, SH3_2, SH3_9, SR-25, Tweety
`ENSMUSG00000005078`	Jkamp	JNK1/MAPK8-associated membrane protein	2	2	1	105.7	Ref	JAMP, TMEM210
`ENSMUSG00000003352`	Cacnb3	Voltage-dependent L-type calcium channel subunit beta-3	3	2	1	21.1	Novel	DUF6374, Guanylate_kin, SH3_1, VGCC_beta4Aa_N
`ENSMUSG00000030108`	Slc6a13	Sodium- and chloride-dependent GABA transporter 2	2	2	1	13.2	Ref	DUF2829, SNF
`ENSMUSG00000033287`	KCTD17	BTB/POZ domain-containing protein KCTD17	3	2	1	74.0	Novel	BTB, BTB_2, SAM_LFY
`ENSMUSG00000030990`	Pgap2	Acyltransferase PGAP2	2	2	1	23.8	Ref	AA_permease_2, Frag1, TMEM138, YtpI
`ENSMUSG00000033216`	Eefsec	Selenocysteine-specific elongation factor	2	2	1	0.0	Novel	DO-GTPase2, FliS_cochap, GTP_EFTU, GTP_EFTU_D2, Gtr1_RagA, MMR_HSR1, PduV-EutP, RIBIOP_C, RsgA_GTPase, SRPRB, cobW
`ENSMUSG00000020327`	Fgf22	Fibroblast growth factor 22	2	2	1	0.0	Ref	DUF4529, FGF, Fascin
`ENSMUSG00000014232`	Cluap1	Clusterin-associated protein 1	2	2	1	52.9	Novel	ATG14, BUD22, CENP-B_dimeris, Cluap1, Jnk-SapK_ap_N, PBP1_TM, RasGAP_C, Tweety
`ENSMUSG00000030401`	Rtn2	Reticulon-2	2	2	1	44.9	Ref	Babuvirus_MP, DUF592, Reticulon, mono-CXXC
`ENSMUSG00000027238`	FRMD5	FERM domain-containing protein 5	2	2	1	7.9	Ref	FA, FERM_C, FERM_M, FERM_N, YcxB
`ENSMUSG00000003873`	Bax	Apoptosis regulator BAX	3	2	1	111.0	Novel	BID, Bcl-2, Bclx_interact, CF222
`ENSMUSG00000028837`	Psmb2	Proteasome subunit beta type-2	2	2	1	380.7	Novel	Proteasome, WSLR
`ENSMUSG00000031068`	Glrx3	Glutaredoxin-3	2	2	1	44.9	Novel	Arf, CNOT1_HEAT_N, DIM1, DUF4279, DUF953, GREB1_C, GST_N_3, GerD, Glutaredoxin, HeH, HyaE, OST3_OST6, Phosducin, RdRP, SH3BGR, TMF_TATA_bd, Thioredoxin, Thioredoxin_2, Thioredoxin_8, Thioredoxin_9, TrbC_Ftype, ZinT
`ENSMUSG00000037740`	Mrps26	Small ribosomal subunit protein mS26	2	2	1	97.8	Ref	DUF1127, MAJIN, MRP-S26, PBP_sp32
`ENSMUSG00000042675`	YPEL3	Protein yippee-like 3	3	2	1	423.0	Mixed	RIG-I_C-RD, Yippee-Mis18
`ENSMUSG00000024926`	Kat5	Histone acetyltransferase KAT5	3	2	1	44.9	Ref	Acetyltransf_1, Acetyltransf_7, MOZ_SAS, Tudor-knot, zf-MYST
`ENSMUSG00000026254`	Eif4e2	Eukaryotic translation initiation factor 4E type 2	4	2	1	13.2	Ref	FlgD, IF4E, IHABP4_N, Stm1_N
`ENSMUSG00000039233`	Tbce	Tubulin-specific chaperone E	2	2	1	34.4	Ref	CAP_GLY, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, Ubiquitin_2
`ENSMUSG00000027593`	Raly	RNA-binding protein Raly	3	2	1	126.9	Ref	RRM_1, RRM_5
`ENSMUSG00000020308`	Tpgs1	Tubulin polyglutamylase complex subunit 1	2	2	1	203.6	Novel	DUF2267, Dpy-30, RIIa
`ENSMUSG00000019738`	POLR2I	DNA-directed RNA polymerase II subunit RPB9	2	2	1	71.4	Ref	CpXC, DUF3716, DZR, Ogr_Delta, RNA_POL_M_15KD, TFIIS_C, TF_Zn_Ribbon, Zn-ribbon_8, Zn_Tnp_IS1595, Zn_Tnp_IS91, Zn_ribbon_recom
`ENSMUSG00000027206`	COPS2	COP9 signalosome complex subunit 2	2	2	1	81.9	Ref	FliS, PCI, TPR_1, TPR_2, TPR_7, YozE_SAM_like
`ENSMUSG00000024048`	Rlc-a	Myosin regulatory light chain RLC-A	2	2	1	87.2	Ref	Ccdc124, DDE_Tnp_IS66_C, DUF3741, EF-hand_1, EF-hand_11, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, GPHH, SPARC_Ca_bdg, SurA_N_2, Syntaxin, UPF0154
`ENSMUSG00000052926`	Rnaseh2a	Ribonuclease H2 subunit A	3	2	1	55.5	Ref	ALG11_N, RNase_HII
`ENSMUSG00000001211`	Agpat3	1-acyl-sn-glycerol-3-phosphate acyltransferase gamma	2	2	1	52.9	Ref	Acyltransf_C, Acyltransferase, Phage_glycop_gL
`ENSMUSG00000024845`	Tmem134	Transmembrane protein 134	3	2	1	79.3	Ref	TMEM_230_134
`ENSMUSG00000000743`	Chmp1a	Charged multivesicular body protein 1a	2	2	1	156.0	Novel	CsiV, Cytochrom_B562, DUF6247, Enkurin, Fes1, Ist1, Josephin, Snf7
`ENSMUSG00000021773`	Comtd1	Catechol O-methyltransferase domain-containing protein 1	2	2	1	13.2	Ref	DNA_binding_1, MTS, Methyltransf_18, Methyltransf_24, Methyltransf_25, Methyltransf_3, Methyltransf_31, PCMT
`ENSMUSG00000060038`	Dhps	Deoxyhypusine synthase	5	2	1	129.5	Mixed	Carbam_trans_N, DS
`ENSMUSG00000039278`	Pcsk1n	ProSAAS	2	2	1	44.9	Novel	FUSC, ProSAAS
`ENSMUSG00000053291`	Rab4b	Ras-related protein Rab-4B	2	2	1	264.4	Ref	AAA_16, AAA_22, AAA_7, ATPase, ATPase_2, Arf, DUF2075, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB, Septin, UvrD-helicase, cobW
`ENSMUSG00000026643`	Nmt2	Glycylpeptide N-tetradecanoyltransferase 2	2	2	1	52.9	Ref	Acetyltransf_1, Acetyltransf_9, NMT, NMT_C, TFIIF_alpha
`ENSMUSG00000030922`	Lyrm1	LYR motif-containing protein 1	2	2	1	7.9	Ref	Complex1_LYR, Complex1_LYR_2, DUF948, SKA2
`ENSMUSG00000028411`	Aptx	Aprataxin	2	2	1	44.9	Ref	CBP_BcsO, DcpS_C, FHA, FHA_2, Glycoamylase, HIT, TAF8_C, zf-AN1, zf-C2H2_4, zf-C2H2_6, zf-C2HE
`ENSMUSG00000004267`	Eno2	Gamma-enolase	2	2	1	372.7	Ref	Enolase_C, Enolase_N, IAT_beta, MAAL_C, MR_MLE_C
`ENSMUSG00000006418`	Rnf114	E3 ubiquitin-protein ligase RNF114	2	2	1	89.9	Ref	DND1_DSRM, DUF5810, DZR_2, FOXP-CC, Methyltransf_15, Prok-RING_4, Rtf2, U-box, zf-BED, zf-C2H2, zf-C2H2_12, zf-C2H2_4, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Di19, zf-H2C2_2, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX, zf_C2HC_14
`ENSMUSG00000033272`	Slc35a4	Probable UDP-sugar transporter protein SLC35A4	3	2	1	15.9	Novel	DUF4535, EamA, Nuc_sug_transp
`ENSMUSG00000018322`	Tomm34	Mitochondrial import receptor subunit TOM34	2	2	1	129.5	Ref	ANAPC3, BTAD, DUF4919, Fis1_TPR_C, MIT, PSII_Pbs27, SHNi-TPR, SRP_TPR_like, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9, TPR_MalT
`ENSMUSG00000079317`	TRAPPC2	Trafficking protein particle complex subunit 2	2	2	1	171.8	Ref	DUF4984, Sedlin_N, Sybindin
`ENSMUSG00000029440`	Psmd9	26S proteasome non-ATPase regulatory subunit 9	2	2	1	7.9	Ref	DUF4133, GRASP55_65, Nas2_N, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000026696`	VAMP4	Vesicle-associated membrane protein 4	2	2	1	34.4	Ref	DUF1664, DUF948, EMP24_GP25L, Fzo_mitofusin, MCU, NPV_P10, Nuf2_DHR10-like, Synaptobrevin, TFR_dimer
`ENSMUSG00000015488`	Cacfd1	Calcium channel flower homolog	3	2	1	29.1	Ref	Bacteriocin_IIc, COPI_assoc, Cg6151-P, TMEM72
`ENSMUSG00000069601`	ANK3	Ankyrin-3	2	2	1	42.3	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, Asp_protease_2, Death, HTH_23, IlvN, RbsD_FucU, Staufen_C, UPA_2, ZU5
`ENSMUSG00000024026`	Glo1	Lactoylglutathione lyase	3	2	1	89.9	Ref	Glyoxalase, Glyoxalase_4, Glyoxalase_6, PIG-X
`ENSMUSG00000031516`	Dctn6	Dynactin subunit 6	2	2	1	304.0	Novel	Fucokinase, Hexapep
`ENSMUSG00000043445`	Pgp	Glycerol-3-phosphate phosphatase	2	2	1	52.9	Ref	Asp_Glu_race, HAD_2, Hydrolase, Hydrolase_6, Hydrolase_like, PGP_phosphatase
`ENSMUSG00000054021`	Sirt5	NAD-dependent protein deacylase sirtuin-5, mitochondrial	3	2	1	31.7	Mixed	GlgS, PNTB, RseA_N, SIR2
`ENSMUSG00000021532`	Fastkd3	FAST kinase domain-containing protein 3, mitochondrial	2	2	1	10.6	Novel	FAST_1, FAST_2, RAP
`ENSMUSG00000025512`	Chid1	Chitinase domain-containing protein 1	2	2	1	2.6	Ref	CoA_binding_2, Glyco_hydro_18, Glyco_hydro_85
`ENSMUSG00000060743`	His3.3A	Histone H3.3A	2	2	1	158.6	Ref	CBFD_NFYB_HMF, CENP-S, CENP-T_C, GHL13, Histone, PAF
`ENSMUSG00000024436`	Mrps18b	Small ribosomal subunit protein mS40	2	2	1	79.3	Ref	FAM165, HTH_21, Ribosomal_S18, zf-RING_10
`ENSMUSG00000022800`	Fyttd1	UAP56-interacting factor	2	2	1	13.2	Ref	DUF1583_N, FYTT, RNA_bind, Rpp20
`ENSMUSG00000055447`	Cd47	Leukocyte surface antigen CD47	4	2	1	89.9	Ref	CD47, Ig_3, Mpo1-like, TssN, V-set_CD47
`ENSMUSG00000024194`	Cuta	Protein CutA	6	2	1	174.5	Mixed	CutA1, DAHP_synth_1
`ENSMUSG00000029048`	Rer1	Protein RER1	2	2	1	50.2	Ref	DUF2208, Rer1, SEP
`ENSMUSG00000026058`	Khdrbs2	KH domain-containing, RNA-binding, signal transduction-associated protein 2	2	2	1	5.3	Ref	KH_1, Qua1, STAR_dimer, Sam68-YY
`ENSMUSG00000039745`	Htatip2	Protein HTATIP2	3	2	1	21.1	Mixed	Epimerase, NAD_binding_10, NAD_binding_4, Sacchrp_dh_NADP, Semialdhyde_dh
`ENSMUSG00000030795`	Fus	RNA-binding protein FUS	2	2	1	18.5	Ref	KleE, RRM_1, zf-RanBP
`ENSMUSG00000031948`	Kars1	Lysine--tRNA ligase	2	2	1	52.9	Ref	DUF4407, HSP70, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon
`ENSMUSG00000049600`	Zbtb45	Zinc finger and BTB domain-containing protein 45	2	2	1	31.7	Novel	BTB, DUF2256, HypA, LIM, Zn-ribbon_8, Zn_Tnp_IS1595, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HC_2, zf-Di19, zf-H2C2_2, zf-UBP, zf-met, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000016481`	Cr1l	Complement component receptor 1-like protein	2	2	1	29.1	Ref	ASFV_J13L, Adeno_E3_CR2, Cyt_c_ox_IV, DUF3290, EphA2_TM, MP_p6, PspB, RIFIN, Sushi, Vpu, cEGF
`ENSMUSG00000016503`	Gtf3a	Transcription factor IIIA	2	2	1	81.9	Ref	C1_2, CCAP, CHORD, FOXP-CC, Ribosomal_S14, Spt46, zf-C2H2, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_aberr, zf-C2H2_jaz, zf-C2HC_2, zf-C2HE, zf-Di19, zf-H2C2_2, zf-H2C2_5, zf-met, zf_Hakai, zf_ZIC
`ENSMUSG00000036775`	Decr2	Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]	2	2	1	31.7	Ref	DUF4675, F420_oxidored, KR, adh_short, adh_short_C2
`ENSMUSG00000003949`	Hlf	Hepatic leukemia factor	2	2	1	81.9	Novel	Cnn_1N, DUF4140, Ish1, bZIP_1, bZIP_2, bZIP_Maf
`ENSMUSG00000050914`	Ankrd37	Ankyrin repeat domain-containing protein 37	2	2	1	37.0	Novel	Ank, Ank_2, Ank_3, Ank_4, Ank_5, Myf5
`ENSMUSG00000021619`	Atg10	Ubiquitin-like-conjugating enzyme ATG10	2	2	1	29.1	Ref	Autophagy_act_C, zinc_ribbon_4
`ENSMUSG00000075704`	Txnrd2	Thioredoxin reductase 2, mitochondrial	2	2	1	26.4	Ref	AlaDh_PNT_C, DAO, FAD_binding_2, FAD_oxidored, GIDA, HI0933_like, Lycopene_cycl, Lys_Orn_oxgnase, NAD_binding_8, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Pyr_redox_dim, Thi4
`ENSMUSG00000056770`	Setd3	Actin-histidine N-methyltransferase	2	2	1	68.7	Novel	Pertussis_S2S3, Rubis-subs-bind, SET
`ENSMUSG00000042472`	Znf410	Zinc finger protein 410	2	2	1	23.8	Ref	CHORD, FYVE, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_aberr, zf-C2H2_jaz, zf-C2HC_2, zf-H2C2_2, zf-RRN7, zf-met
`ENSMUSG00000022681`	Ntan1	Protein N-terminal asparagine amidohydrolase	9	2	1	103.1	Novel	FIST, N_Asn_amidohyd
`ENSMUSG00000020585`	Laptm4a	Lysosomal-associated transmembrane protein 4A	2	2	1	729.6	Novel	Circovir2_Orf4, DUF4728, Mtp
`ENSMUSG00000032580`	Rbm5	RNA-binding protein 5	3	2	1	10.6	Ref	G-patch, G-patch_2, GSIII_N, OCRE, PHM7_cyt, RRM_1, zf-RanBP
`ENSMUSG00000040888`	Dst	Dystonin	2	2	1	7.9	Ref	APOBEC4, DUF5344, DUF6730, Evr1_Alr, FAD_SOX, GrpE, NCKAP5, Nbl1_Borealin_N, SNAD4, Spectrin
`ENSMUSG00000050471`	Fam118b	Protein FAM118B	2	2	1	2.6	Ref	DUF5443, SIR2_2
`ENSMUSG00000014767`	Tbp	TATA-box-binding protein	2	2	1	7.9	Ref	ANAPC_CDC26, AcnX_swivel_put, Pox_A6, Presenilin, TBP, TFIIA, Tombus_movement, zf-HIT, zf-MYND
`ENSMUSG00000023806`	Rsph3a	Radial spoke head protein 3 homolog A	3	2	1	113.7	Ref	Radial_spoke_3
`ENSMUSG00000022634`	Yaf2	YY1-associated factor 2	2	2	1	44.9	Ref	C1_2, Ephrin_rec_like, PROCN, RAP1, WTX, YAF2_RYBP, zf-RanBP
`ENSMUSG00000090266`	Mettl23	Histone-arginine methyltransferase METTL23	3	2	1	68.7	Ref	Methyltransf_16
`ENSMUSG00000058672`	TUBB2A	Tubulin beta-2A chain	2	2	1	2,366.0	Novel	Misat_Tub_SegII, Tubulin, Tubulin_2, Tubulin_3, Tubulin_C
`ENSMUSG00000019302`	Atp6v0a1	V-type proton ATPase 116 kDa subunit a 1	2	2	1	52.9	Ref	AlkA_N, CCDC85, V_ATPase_I
`ENSMUSG00000039323`	Igfbp2	Insulin-like growth factor-binding protein 2	3	2	1	50.2	Mixed	Cys_rich_CWC, Thyroglobulin_1
`ENSMUSG00000018442`	Derl2	Derlin-2	2	2	1	21.1	Ref	DER1, Oleosin
`ENSMUSG00000042271`	Nxt2	NTF2-related export protein 2	2	2	1	23.8	Ref	NTF2, QRPTase_N
`ENSMUSG00000026163`	Sphkap	A-kinase anchor protein SPHKAP	2	2	1	58.2	Ref	AKAP_110, DUF1133, FAM212, MAGP
`ENSMUSG00000030707`	Coro1a	Coronin-1A	3	2	1	214.1	Novel	AKNA, ANAPC4_WD40, BLOC1_2, DUF1465, DUF1899, NBCH_WD40, Trimer_CC, WD40, WD40_4, XhlA, eIF2A
`ENSMUSG00000033732`	Sf3b3	Splicing factor 3B subunit 3	2	2	1	10.6	Ref	CPSF_A, MMS1_N
`ENSMUSG00000030706`	Mrpl48	Large ribosomal subunit protein mL48	3	2	1	21.1	Novel	Ribosomal_S10
`ENSMUSG00000039128`	Cdc123	Translation initiation factor eIF2 assembly protein	2	2	1	39.7	Novel	D123, Reg_prop
`ENSMUSG00000028657`	Ppt1	Palmitoyl-protein thioesterase 1	2	2	1	47.6	Ref	Abhydrolase_1, Palm_thioest, U71
`ENSMUSG00000027489`	Necab3	N-terminal EF-hand calcium-binding protein 3	2	2	1	97.8	Novel	ABM, Cactin_mid, DnaI_N, EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, Jnk-SapK_ap_N
`ENSMUSG00000026833`	Olfm1	Noelin	2	2	1	756.1	Ref	BLOC1_2, CENP-Q, CLZ, Csm1_N, DUF3450, Enkurin, Ephrin, FtsZ_C, MitMem_reg, Noelin-1, PilJ, SlyX, XhlA, ZapB
`ENSMUSG00000031791`	Tmem38a	Trimeric intracellular cation channel type A	2	2	1	148.0	Novel	Metal_resist, TRIC
`ENSMUSG00000057531`	Dtnbp1	Dysbindin	2	2	1	63.4	Ref	DUF2730, Dysbindin, FUSC, Peptidase_S46, TRPM_tetra
`ENSMUSG00000042726`	Trafd1	TRAF-type zinc finger domain-containing protein 1	2	2	1	29.1	Ref	PIP49_C, TRAF6_Z2, XAF1_C, zf-TRAF, zf_UBZ
`ENSMUSG00000018398`	Septin8	Septin-8	3	2	1	182.4	Ref	AAA_16, AAA_22, AAA_23, AAA_24, AAA_29, AAA_7, ABC_tran, AIG1, ATG14, ATP_bind_1, DUF5906, DUF87, Dynamin_N, GTP_EFTU, IMD, MMR_HSR1, RHD3_GTPase, RNA_helicase, Ras, Roc, RsgA_GTPase, Septin, T2SSE
`ENSMUSG00000019478`	Rab4a	Ras-related protein Rab-4A	2	2	1	97.8	Novel	AAA_16, AAA_22, AAA_7, ATP_bind_1, ATPase, ATPase_2, Arf, DUF2075, GTP_EFTU, Gtr1_RagA, MMR_HSR1, NACHT, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB, Septin
`ENSMUSG00000005338`	Cadm3	Cell adhesion molecule 3	2	2	1	55.5	Ref	C1-set, C2-set, C2-set_2, DEC1, I-set, Ig_2, Ig_3, Ig_4, Izumo-Ig, SBD, Syndecan, V-set, ig
`ENSMUSG00000020083`	Fam241b	Protein FAM241B	3	2	1	97.8	Ref	DUF4605
`ENSMUSG00000020289`	Nprl3	GATOR1 complex protein NPRL3	2	2	1	5.3	Ref	NPR2, NPR3, Pur_DNA_glyco
`ENSMUSG00000005481`	Ddx39a	ATP-dependent RNA helicase DDX39A	4	2	1	15.9	Ref	CMS1, DEAD, Helicase_C, ResIII
`ENSMUSG00000045969`	Ing1	Inhibitor of growth protein 1	2	2	1	18.5	Ref	CCDC106, CDC45, CIS_TMP, DDHD, DUF4661, DUF6739, Dsh_C, Hid1, ING, KH_5, MAS20, MCM_bind, NPR3, PHD, PHD_2, Presenilin, SRP-alpha_N, Snu56_snRNP, ZYG-11_interact, Zip, zf-C4pol, zf-HC5HC2H
`ENSMUSG00000056228`	Cars2	Probable cysteine--tRNA ligase, mitochondrial	3	2	1	15.9	Ref	DALR_2, tRNA-synt_1, tRNA-synt_1e, tRNA-synt_1f, tRNA-synt_1g
`ENSMUSG00000025508`	Rplp2	Large ribosomal subunit protein P2	2	2	1	76.7	Ref	EF-hand_14, NolX, RelA_AH_RIS, Ribosomal_60s, TFIID_30kDa
`ENSMUSG00000033735`	Spr	Sepiapterin reductase	2	2	1	195.6	Ref	BRINP, KR, Nodulin-like, adh_short, adh_short_C2
`ENSMUSG00000004070`	Hmox2	Heme oxygenase 2	3	2	1	55.5	Mixed	CENP-H, Heme_oxygenase, SsgA
`ENSMUSG00000022257`	Laptm4b	Lysosomal-associated transmembrane protein 4B	2	2	1	203.6	Ref	AC_N, DUF4728, DUF6419, Mtp, Saf_2TM, Tetraspanin
`ENSMUSG00000042787`	Exog	Nuclease EXOG, mitochondrial	3	2	1	5.3	Ref	DUF6730, Endonuclease_NS, Exog_C, YvrJ
`ENSMUSG00000034757`	Tmub2	Transmembrane and ubiquitin-like domain-containing protein 2	3	2	1	84.6	Ref	CD52, DUF6232, Dsc3_N, Rad60-SLD_2, ubiquitin
`ENSMUSG00000051504`	CD33	Myeloid cell surface antigen CD33	3	2	1	21.1	Ref	C2-set_2, DUF2490, I-set, Ig_3, V-set, ig
`ENSMUSG00000026095`	Asnsd1	Asparagine synthetase domain-containing protein 1	2	2	1	39.7	Ref	Asn_synthase, GATase_6, GATase_7, NAD_synthase, Prefoldin_2
`ENSMUSG00000022223`	Sdr39u1	Epimerase family protein SDR39U1	2	2	1	163.9	Novel	DUF1731, Epimerase, GDP_Man_Dehyd, NAD_binding_10, Pyr_redox, RmlD_sub_bind
`ENSMUSG00000020024`	Cep83	Centrosomal protein of 83 kDa	2	2	1	10.6	Ref	FtsL_2, HTH_53
`ENSMUSG00000004069`	Dnaja3	DnaJ homolog subfamily A member 3, mitochondrial	2	2	1	34.4	Ref	Anti-TRAP, DnaJ, DnaJ_C, DnaJ_CXXCXGXG, Endonuclease_7, FtrD-like
`ENSMUSG00000055762`	Eef1d	Elongation factor 1-delta	5	2	1	71.4	Mixed	CCD48, EF-1_beta_acid, EF1_GNE, JIP_LZII, KASH_CCD, Lzipper-MIP1, PRKG1_interact, PilJ, RF-1, Striatin, TSC22, TolA_bind_tri, Trimer_CC
`ENSMUSG00000073411`	H2-D1	H-2 class I histocompatibility antigen, D-B alpha chain	2	2	1	50.2	Ref	C1-set, C2-set_2, Ig_2, Ig_3, MHC_I, MHC_I_C, Omp85, ig
`ENSMUSG00000005986`	Ankrd13d	Ankyrin repeat domain-containing protein 13D	2	2	1	39.7	Novel	Ank, Ank_2, Ank_3, Ank_4, Ank_5, GPCR_chapero_1, RAP80_UIM, UIM
`ENSMUSG00000031168`	Ebp	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase	2	2	1	103.1	Novel	EBP, PET122
`ENSMUSG00000021493`	PDLIM7	PDZ and LIM domain protein 7	5	2	1	100.5	Mixed	PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000025591`	Tma16	Translation machinery-associated protein 16	2	2	1	2.6	Ref	DUF1910, Tma16
`ENSMUSG00000034563`	Ccpg1	Cell cycle progression protein 1	3	2	1	47.6	Ref	Csm1_N, DHR-2_Lobe_C, YtxH
`ENSMUSG00000001127`	Araf	Serine/threonine-protein kinase A-Raf	5	2	1	237.9	Novel	C1_1, RBD, TUG-UBL1, zf-RING-like, zf-RING_2
`ENSMUSG00000037032`	Apbb1	Amyloid beta precursor protein binding family B member 1	5	2	1	446.8	Ref	PID, PID_2, PTB, WW
`ENSMUSG00000036381`	P2ry14	P2Y purinoceptor 14	2	2	1	2.6	Ref	7tm_1, Bac_export_2, DUF4834, Ni_hydr_CYTB, PDR_CDR, TMIE
`ENSMUSG00000047084`	Ngrn	Neugrin	2	2	1	95.2	Ref	Dermcidin, MRP-L20, Neugrin, Ribosomal_L29, UPF0122
`ENSMUSG00000015749`	Anp32e	Acidic leucine-rich nuclear phosphoprotein 32 family member E	2	2	1	34.4	Ref	BUD22, FBXL18_LRR, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, SAPS
`ENSMUSG00000032869`	Psmf1	Proteasome inhibitor PI31 subunit	2	2	1	15.9	Ref	PI31_Prot_C, PI31_Prot_N
`ENSMUSG00000029359`	Tesc	Calcineurin B homologous protein 3	2	2	1	214.1	Ref	EF-hand_1, EF-hand_2, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, GIT_CC, M16C_assoc
`ENSMUSG00000032425`	ZNF717	Zinc finger protein 717	2	2	1	2.6	Novel	ADK_lid, BolA, C1_4, DUF5830, GAGA, KRAB, MciZ, XPA_N, Zn-ribbon_8, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-LYAR, zf-met, zinc_ribbon_15
`ENSMUSG00000033595`	Lgi3	Leucine-rich repeat LGI family member 3	2	2	1	68.7	Ref	EPTP, LRRCT, LRR_1, LRR_4, LRR_5, LRR_8, LRR_9, RCDG1, stn_TNFRSF12A
`ENSMUSG00000025867`	CPLX2	Complexin-2	2	2	1	544.6	Novel	AP-5_subunit_s1, Borrelia_P83, DDHD, DUF2838, DUF4407, MDM10, RNA_polI_A34, RR_TM4-6, Spt5_N, Synaphin, VIT1
`ENSMUSG00000025534`	Gusb	Beta-glucuronidase	2	2	1	7.9	Ref	Glyco_hydro_2, Glyco_hydro_2_C, Glyco_hydro_2_N
`ENSMUSG00000039660`	Spout1	28S rRNA (uridine-N(3))-methyltransferase	2	2	1	42.3	Ref	AP1AR, DUF2514, Methyltrn_RNA_3
`ENSMUSG00000035443`	Thyn1	Thymocyte nuclear protein 1	6	2	1	92.5	Mixed	EVE
`ENSMUSG00000018405`	Mrm1	rRNA methyltransferase 1, mitochondrial	2	2	1	7.9	Novel	Methyltrn_RNA_4, SpoU_methylase, SpoU_sub_bind
`ENSMUSG00000019232`	Etnppl	Ethanolamine-phosphate phospho-lyase	2	2	1	2.6	Novel	Aminotran_3, HAD_2, NUC130_3NT
`ENSMUSG00000008305`	Tle1	Transducin-like enhancer protein 1	2	2	1	10.6	Ref	ANAPC4_WD40, Cytochrom_D1, HALZ, NBCH_WD40, Nup160, Ribosomal_S18, Spc24, TLE_N, Transketolase_N, WD40, WD40_like
`ENSMUSG00000026333`	Gin1	Gypsy retrotransposon integrase-like protein 1	2	2	1	10.6	Ref	Cytochrom_D1, Integrase_H2C2, rve, zf-H2C2
`ENSMUSG00000028063`	Lmna	Prelamin-A/C	2	2	1	108.4	Ref	ATG16, CENP-F_leu_zip, Cnn_1N, Crescentin, DUF4407, FIVAR, Fez1, Filament, GBR2_CC, LTD, TPR_MLP1_2, Tht1, TolA_bind_tri, YabA, zf-NADH-PPase
`ENSMUSG00000005312`	Ubqln1	Ubiquilin-1	2	2	1	23.8	Ref	CUE, ComX, DnaG_DnaB_bind, Dsc3_N, HOIP-UBA, PDH_C, Rad60-SLD, Rad60-SLD_2, STI1, UBA, Ubiquitin_4, Ubiquitin_5, XPC-binding, YukD, ubiquitin
`ENSMUSG00000041199`	Rpusd1	RNA pseudouridylate synthase domain-containing protein 1	4	2	1	92.5	Ref	PseudoU_synth_2
`ENSMUSG00000039217`	Il18	Interleukin-18	2	2	1	13.2	Novel	DUF273, IL1, NCA2
`ENSMUSG00000039917`	Rhbdd2	Rhomboid domain-containing protein 2	2	2	1	10.6	Ref	DER1, DUF2070, Rhomboid
`ENSMUSG00000059810`	Rgs3	Regulator of G-protein signaling 3	2	2	1	7.9	Novel	DUF4441, RGS
`ENSMUSG00000073079`	Srp54	Signal recognition particle subunit SRP54	2	2	1	18.5	Ref	6PF2K, AAA, AAA_16, AAA_17, AAA_18, AAA_22, AAA_28, AAA_30, AAA_31, AAA_33, APS_kinase, ATP_bind_1, ArsA_ATPase, CLP1_P, CbiA, DUF2209, DUF826, G-alpha, MMR_HSR1, MeaB, MobB, PduV-EutP, ResIII, SRP54, SRP54_N, SRPRB, SRP_SPB, Thymidylate_kin, Torsin, Zeta_toxin, cobW
`ENSMUSG00000026921`	Egfl7	Epidermal growth factor-like protein 7	4	2	1	34.4	Mixed	EGF, EGF_2, EGF_CA, EMI, SlyX
`ENSMUSG00000031371`	Haus7	HAUS augmin-like complex subunit 7	2	2	1	21.1	Ref	AATF-Che1, FlgN, Nsp1_C, Plant_NMP1, YabA
`ENSMUSG00000024853`	Sf3b2	Splicing factor 3B subunit 2	2	2	1	89.9	Ref	DUF382, PSP, SAP, SAP_new25
`ENSMUSG00000029763`	Exoc4	Exocyst complex component 4	2	2	1	23.8	Ref	COG5, Cauli_AT, DASH_Duo1, Dor1, Ead_Ea22, PEP-utilisers_N, Prominin, Sec8_exocyst, T3SS_needle_E, Vps51, Vps54_N, Zw10
`ENSMUSG00000039419`	Cntnap2	Contactin-associated protein-like 2	2	2	1	13.2	Ref	Adeno_E3_CR2, COLFI, EGF, F5_F8_type_C, Laminin_G_1, Laminin_G_2, Laminin_G_3, MAP17, MARVEL, Syndecan, Tmemb_9, UstYa, hEGF
`ENSMUSG00000041353`	FAM156A	Protein FAM156A/FAM156B	2	2	1	26.4	Ref	FAM104, PGC7_Stella
`ENSMUSG00000005362`	Crbn	Protein cereblon	2	2	1	23.8	Ref	Baculo_ME53, LON_substr_bdg, Yippee-Mis18
`ENSMUSG00000021236`	Entpd5	Ectonucleoside triphosphate diphosphohydrolase 5	2	2	1	5.3	Ref	GDA1_CD39, zf-rbx1
`ENSMUSG00000026270`	Capn10	Calpain-10	2	2	1	37.0	Ref	Calpain_III, Peptidase_C2
`ENSMUSG00000014602`	Kif1a	Kinesin-like protein KIF1A	2	2	1	293.4	Ref	DUF3694, FHA, KIF1B, Kinesin, Kinesin_assoc, Microtub_bd, PH, PH_11, Pox_Ag35, Yop-YscD_cpl
`ENSMUSG00000019254`	Ppp1r12c	Protein phosphatase 1 regulatory subunit 12C	2	2	1	2.6	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, PRKG1_interact
`ENSMUSG00000040667`	Nup88	Nuclear pore complex protein Nup88	2	2	1	26.4	Ref	BLOC1_2, DUF4208, DUF4404, Exonuc_VII_L, FliJ, Importin_rep_6, Latarcin, Nup88
`ENSMUSG00000031697`	Orc6	Origin recognition complex subunit 6	2	2	1	74.0	Ref	Cyclin_C, EAD9, ORC6
`ENSMUSG00000032482`	Cspg5	Chondroitin sulfate proteoglycan 5	4	2	1	198.3	Ref	Chon_Sulph_att, Neural_ProG_Cyt
`ENSMUSG00000024873`	CNIH2	Protein cornichon homolog 2	2	2	1	148.0	Novel	Cornichon, PhoR
`ENSMUSG00000026811`	St6galnac6	Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6	2	2	1	55.5	Novel	Cytochrome-c551, Glyco_transf_29
`ENSMUSG00000001844`	Zdhhc4	Palmitoyltransferase ZDHHC4	3	2	1	15.9	Novel	DHHC
`ENSMUSG00000025264`	Tsr2	Pre-rRNA-processing protein TSR2 homolog	2	2	1	31.7	Ref	DUF6279, LPD22, WGG
`ENSMUSG00000078695`	Cisd3	CDGSH iron-sulfur domain-containing protein 3, mitochondrial	3	2	1	116.3	Ref	Inhibitor_I71, Toxin_14, zf-CDGSH
`ENSMUSG00000024797`	Vps51	Vacuolar protein sorting-associated protein 51 homolog	2	2	1	21.1	Ref	COG2, COG5, DUF3829, Dor1, GIT_CC, Occludin_ELL, Sec5, TMTC_DUF1736, Vps51, Vps53_N, Vps54_N, Zw10
`ENSMUSG00000025245`	Lztfl1	Leucine zipper transcription factor-like protein 1	2	2	1	13.2	Ref	DNA_pol3_a_NI, FUSC, Hom_end_hint, Leu_zip, MAD, Methyltransf_14, Mit_ribos_Mrp51, SesA
`ENSMUSG00000029780`	Nt5c3a	Cytosolic 5'-nucleotidase 3A	2	2	1	66.1	Ref	ACT_7, CIDE-N, DZR_2, HAD, Hydrolase, UMPH-1, zf-ANAPC11
`ENSMUSG00000032504`	Pdcd6ip	Programmed cell death 6-interacting protein	2	2	1	31.7	Ref	AAT, ALIX_LYPXL_bnd, BRO1, DUF5082, Ly49, Tex_N
`ENSMUSG00000025728`	Pigq	Phosphatidylinositol N-acetylglucosaminyltransferase subunit Q	2	2	1	18.5	Ref	DUF6050, Gpi1
`ENSMUSG00000029472`	Anapc5	Anaphase-promoting complex subunit 5	3	2	1	58.2	Ref	ANAPC5, MapZ_EC1, TPR_1, TPR_10, TPR_12, TPR_14, TPR_19, TPR_2, TPR_4, TPR_7, VP4_haemagglut, YycI
`ENSMUSG00000026575`	Nme7	Nucleoside diphosphate kinase homolog 7	2	2	1	7.9	Novel	Arg_repressor, DM10_dom, NDK
`ENSMUSG00000001247`	Lsr	Lipolysis-stimulated lipoprotein receptor	3	2	1	7.9	Ref	LSR, V-set
`ENSMUSG00000028519`	Dab1	Disabled homolog 1	3	2	1	23.8	Novel	Bac_globin, PID
`ENSMUSG00000025495`	Ptdss2	Phosphatidylserine synthase 2	2	2	1	10.6	Ref	DUF3382, Gemini_mov, HemY_N, PSS, TctB
`ENSMUSG00000029152`	Ociad1	OCIA domain-containing protein 1	6	2	1	565.7	Mixed	OCIA
`ENSMUSG00000015094`	Npdc1	Neural proliferation differentiation and control protein 1	2	2	1	1,171.1	Ref	LapA_dom, NPDC1
`ENSMUSG00000074129`	Rpl13a	Large ribosomal subunit protein uL13	2	2	1	1,414.3	Ref	Ribosomal_L13, SLT_L
`ENSMUSG00000031347`	Cetn2	Centrin-2	2	2	1	71.4	Ref	Asp-B-Hydro_N, CNNM, Caleosin, DUF4601, DUF6694, Dockerin_1, EF-hand_1, EF-hand_11, EF-hand_14, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, Fin, HerA_C, LETM1_RBD, RTP, SRI, SUB1_ProdP9, SurA_N_2, SurA_N_3
`ENSMUSG00000049044`	Rapgef4	Rap guanine nucleotide exchange factor 4	2	2	1	23.8	Ref	DEP, RasGEF, RasGEF_N, cNMP_binding
`ENSMUSG00000024369`	Nelfe	Negative elongation factor E	3	2	1	42.3	Ref	Nup35_RRM_2, RPA43_OB, RRM_1, RRM_occluded
`ENSMUSG00000056260`	Lrif1	Ligand-dependent nuclear receptor-interacting factor 1	2	2	1	21.1	Ref	DUF4342, LRIF1, Phage_rep_O, TetR_C_19
`ENSMUSG00000026199`	Ankzf1	tRNA endonuclease ANKZF1	2	2	1	10.6	Ref	ANAPC_CDC26, Ank, Ank_2, Ank_3, Ank_4, Ank_5, Cellulose_synt, DUF2514, DUF3464, Eapp_C, FAM176, FYDLN_acid, HscB_4_cys, Plasmodium_Vir, Presenilin, RNA_pol_3_Rpc31, RPA43_OB, RR_TM4-6, Rrn6, Sec62, SprA-related, TERB2, VATC, bVLRF1, zf-C2H2_jaz
`ENSMUSG00000035505`	Cox18	Cytochrome c oxidase assembly protein COX18, mitochondrial	3	2	1	18.5	Novel	60KD_IMP
`ENSMUSG00000059436`	Max	Protein max	2	2	1	63.4	Ref	ATG16, Csm1_N, DUF4140, DUF4763, DUF5595, DUF5716, DivIC, FapA, Filament, HALZ, HLH, JAKMIP_CC3, Jnk-SapK_ap_N, SLX9, SlyX, V_ATPase_I, ZapB, bZIP_2
`ENSMUSG00000030086`	Chchd6	MICOS complex subunit Mic25	2	2	1	552.5	Ref	ATG14, Activator-TraM, CHCH, DUF1690, DUF3150, DUF4337, DUF6462, Exonuc_VII_L, FapA, IZUMO, MIC19_MIC25, NDUF_B7, hDGE_amylase
`ENSMUSG00000006675`	P4htm	Transmembrane prolyl 4-hydroxylase	2	2	1	193.0	Ref	2OG-FeII_Oxy_3, EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, TruB_C
`ENSMUSG00000015804`	Med28	Mediator of RNA polymerase II transcription subunit 28	2	2	1	81.9	Ref	DUF6530, Filament, HSCB_C, HTH_34, Med28, NPV_P10, PSD2, Sec2p
`ENSMUSG00000027193`	Api5	Apoptosis inhibitor 5	2	2	1	44.9	Ref	API5, Adaptin_N, CAS_CSE1, DUF3966, Dak2, HEAT_2, MMS19_N
`ENSMUSG00000028576`	Ift74	Intraflagellar transport protein 74 homolog	2	2	1	29.1	Novel	ATG17_like, Crescentin, DUF1192, Fez1, Spc7, Spectrin
`ENSMUSG00000026430`	Rassf5	Ras association domain-containing protein 5	2	2	1	5.3	Ref	4HB_MCP_1, C1_1, C1_2, LexA_DNA_bind, Mst1_SARAH, Myb_DNA-bind_4, Nore1-SARAH, PHD, RA
`ENSMUSG00000037089`	Slc35b2	Adenosine 3'-phospho 5'-phosphosulfate transporter 1	2	2	1	42.3	Ref	DUF2070, EamA, PI3K_rbd, TPT, UAA
`ENSMUSG00000063802`	Hspbp1	Hsp70-binding protein 1	4	2	1	237.9	Novel	Arm, CLIP_SPH_mas, Fes1, HEAT, HEAT_EZ, PPTA, Rcd1
`ENSMUSG00000024957`	Kcnk4	Potassium channel subfamily K member 4	2	2	1	10.6	Novel	G3P_acyltransf, IRK, Ion_trans, Ion_trans_2, PDZ_3
`ENSMUSG00000067873`	Htatsf1	17S U2 SnRNP complex component HTATSF1	2	2	1	37.0	Ref	Nup35_RRM, RRM_1, RRM_7
`ENSMUSG00000034744`	Nagk	N-acetyl-D-glucosamine kinase	5	2	1	81.9	Novel	BcrAD_BadFG, MscS_TM
`ENSMUSG00000048967`	Yjefn3	YjeF N-terminal domain-containing protein 3	2	2	1	29.1	Novel	DUF4381, GRIM-19, Planc_extracel, YjeF_N
`ENSMUSG00000038880`	Mrps34	Small ribosomal subunit protein mS34	2	2	1	169.2	Ref	MRP-S34, NDK, PSI_PsaJ, Peptidase_S77
`ENSMUSG00000019906`	Lin7a	Protein lin-7 homolog A	2	2	1	18.5	Ref	7TMR-HDED, CENP-Q, CREPT, DUF932, L27, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Tricorn_PDZ
`ENSMUSG00000030032`	Wdr54	WD repeat-containing protein 54	2	2	1	37.0	Ref	ANAPC4_WD40, Anillin, Lgl_C, PQQ, RAB3GAP2_N, WD40
`ENSMUSG00000066456`	Hmgn3	High mobility group nucleosome-binding domain-containing protein 3	2	2	1	261.7	Ref	HMG14_17, TT_ORF2, UPF0239
`ENSMUSG00000025646`	Atrip	ATR-interacting protein	2	2	1	15.9	Ref	FapA, Fez1, Golgin_A5, HIP1_clath_bdg, HMMR_N, Lebercilin, Med21, OmpH, RNase_H_2, RNase_T, Tropomyosin_1
`ENSMUSG00000030685`	Kctd13	BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1	2	2	1	235.3	Novel	BTB, BTB_2, BTB_3, DUF4719
`ENSMUSG00000068264`	AP5S1	AP-5 complex subunit sigma-1	2	2	1	10.6	Ref	AP-5_subunit_s1, Corona_5a
`ENSMUSG00000074899`	SPTBN5	Spectrin beta chain, non-erythrocytic 5	2	2	1	2.6	Novel	AHSP, CAMSAP_CH, CH, DUF4248, Spectrin, Spt20
`ENSMUSG00000029126`	Nsg1	Neuronal vesicle trafficking-associated protein 1	3	2	1	129.5	Mixed	Calcyon, DUF1581, DUF2448, DUF535
`ENSMUSG00000033099`	Nol12	Nucleolar protein 12	2	2	1	7.9	Novel	ALC, FKBP_N, Nop25, Nup54_C
`ENSMUSG00000031765`	MT1G	Metallothionein-1G	3	2	1	832.7	Ref	Metallothio
`ENSMUSG00000027286`	Lrrc57	Leucine-rich repeat-containing protein 57	4	2	1	63.4	Ref	CSTF_C, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9
`ENSMUSG00000002625`	Akap8l	A-kinase anchor protein 8-like	2	2	1	50.2	Ref	AKAP95, CDC45, SURF2, zf-C2H2_2
`ENSMUSG00000061613`	U2AF1	Splicing factor U2AF 35 kDa subunit	3	2	1	163.9	Ref	KMP11, Nup35_RRM_2, RRM_1, Torus, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf_CCCH_4
`ENSMUSG00000030605`	Mfge8	Lactadherin	2	2	1	330.4	Ref	EGF, EGF_2, F5_F8_type_C, Laminin_EGF, Muskelin_N, cEGF, hEGF
`ENSMUSG00000036932`	Aifm1	Apoptosis-inducing factor 1, mitochondrial	2	2	1	23.8	Ref	AIF-MLS, AIF_C, Amino_oxidase, DUF3918, FAD_oxidored, HI0933_like, Lys_Orn_oxgnase, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Reductase_C, TPP_enzyme_M, Trp_halogenase
`ENSMUSG00000060036`	Rpl3	Large ribosomal subunit protein uL3	2	2	1	2,757.2	Ref	DUF6709, Ribosomal_L3
`ENSMUSG00000026201`	Stk16	Serine/threonine-protein kinase 16	4	2	1	116.3	Novel	ABC1, APH, Kdo, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, TCAD9
`ENSMUSG00000038387`	Rras	Ras-related protein R-Ras	2	2	1	15.9	Novel	AAA_22, ATP_bind_1, Arf, GTP_EFTU, Leu_Phe_trans, MMR_HSR1, Ras, Ribosomal_S30AE, Roc, RsgA_GTPase
`ENSMUSG00000031536`	Polb	DNA polymerase beta	3	2	1	124.2	Novel	DNA_pol_B_palm, DNA_pol_B_thumb, NTP_transf_2
`ENSMUSG00000068739`	Sars1	Serine--tRNA ligase, cytoplasmic	2	2	1	60.8	Ref	NOD, PG_binding_2, Seryl_tRNA_N, VOMI, tRNA-synt_2b
`ENSMUSG00000039615`	Stub1	E3 ubiquitin-protein ligase CHIP	2	2	1	42.3	Ref	ANAPC3, CHIP_TPR_N, NYD-SP12_N, SHNi-TPR, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_20, TPR_6, TPR_7, TPR_8, TPR_9, U-box, zf-NOSIP
`ENSMUSG00000024142`	Mlst8	Target of rapamycin complex subunit LST8	3	2	1	23.8	Ref	ANAPC4_WD40, BRICHOS, DUF3463, DUF5854, Ge1_WD40, Hira, NBCH_WD40, Nup160, WD40
`ENSMUSG00000028545`	Bend5	BEN domain-containing protein 5	2	2	1	15.9	Ref	AAA_23, BEN, DASH_Spc34, DUF349, DUF5917, DUF6594, Docking, LMBR1, OmpH, PIN_12, Sec8_exocyst, Sipho_Gp157, T4SS, TSNAXIP1_N
`ENSMUSG00000039069`	MTG2	Mitochondrial ribosome-associated GTPase 2	2	2	1	7.9	Ref	Arf, Dynamin_N, FeoB_N, GTP1_OBG, MMR_HSR1, Ras, cobW
`ENSMUSG00000027787`	Nmd3	60S ribosomal export protein NMD3	2	2	1	21.1	Novel	Atrophin-1, DUF3915, DUF4637, DZR, HGTP_anticodon2, NMD3
`ENSMUSG00000097466`	Htr1b	5-hydroxytryptamine receptor 1B	2	2	1	0.0	Ref	7TM_GPCR_Srw, 7TM_GPCR_Srx, 7TM_GPCR_Str, 7tm_1, DUF1725, DUF6773
`ENSMUSG00000031878`	Nae1	NEDD8-activating enzyme E1 regulatory subunit	2	2	1	84.6	Ref	E1_4HB, SEP, ThiF
`ENSMUSG00000033342`	Plppr5	Phospholipid phosphatase-related protein type 5	2	2	1	2.6	Ref	DUF6724, Mid2, PAP2, Syndecan, YwiC
`ENSMUSG00000052026`	Slc6a7	Sodium-dependent proline transporter	2	2	1	21.1	Novel	Phage_holin_2_3, SNF
`ENSMUSG00000060703`	Cd302	CD302 antigen	2	2	1	166.5	Ref	DUF973, IN_DBD_C, Lectin_C, UL45
`ENSMUSG00000028863`	Meaf6	Chromatin modification-related protein MEAF6	3	2	1	21.1	Novel	BBS2_C, NuA4
`ENSMUSG00000090015`	Znf431	Zinc finger protein 431	2	2	1	7.9	Ref	BDHCT_assoc, C1_1, C1_4, KRAB, XPA_N, ZZ, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-Paramyx-P, zf-trcl, zf_UBZ, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000017493`	Igfbp4	Insulin-like growth factor-binding protein 4	3	2	1	10.6	Novel	Thyroglobulin_1
`ENSMUSG00000026701`	Prdx6	Peroxiredoxin-6	3	2	1	391.2	Ref	1-cysPrx_C, AhpC-TSA, Redoxin
`ENSMUSG00000027805`	PFN2	Profilin-2	2	2	1	621.2	Ref	DUF2076, DUF4106, Profilin
`ENSMUSG00000063765`	Chadl	Chondroadherin-like protein	2	2	1	37.0	Ref	LRRCT, LRRNT, LRR_1, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9
`ENSMUSG00000027618`	Nfs1	Cysteine desulfurase	2	2	1	52.9	Ref	Aminotran_5, Beta_elim_lyase, DegT_DnrJ_EryC1, EH_Signature, Peptidase_C48, Pyridoxal_deC
`ENSMUSG00000015126`	Tsr3	18S rRNA aminocarboxypropyltransferase	7	2	1	92.5	Ref	RLI, Ribo_biogen_C
`ENSMUSG00000002996`	Hbp1	HMG box-containing protein 1	2	2	1	5.3	Ref	AXH, HMG_box, HMG_box_2, MATalpha_HMGbox
`ENSMUSG00000029319`	Coq2	4-hydroxybenzoate polyprenyltransferase, mitochondrial	2	2	1	66.1	Novel	DUF2798, UbiA
`ENSMUSG00000078941`	Ak6	Adenylate kinase isoenzyme 6	4	2	1	169.2	Mixed	AAA, AAA_17, AAA_18, AAA_22, AAA_28, AAA_33, ADK, Bromo_TP, CENP-S, CENP-T_C, Cytidylate_kin, Cytidylate_kin2, DUF2125, DUF3245, DUF4611, Histone, LonB_AAA-LID, RNA_helicase, SKI, TFIID-31kDa, dNK
`ENSMUSG00000048796`	Cyb561d1	Probable transmembrane reductase CYB561D1	2	2	1	5.3	Ref	Cytochrom_B561, DUF5325, DUF6249
`ENSMUSG00000059743`	Fdps	Farnesyl pyrophosphate synthase	2	2	1	317.2	Ref	ANIS5_cation-bd, polyprenyl_synt
`ENSMUSG00000036748`	Cuedc2	CUE domain-containing protein 2	7	2	1	306.7	Mixed	CUE, DUF6592
`ENSMUSG00000058799`	Nap1l1	Nucleosome assembly protein 1-like 1	3	2	1	417.7	Novel	NAP
`ENSMUSG00000021750`	Fam107a	Actin-associated protein FAM107A	6	2	1	7.9	Novel	DUF1151, KIAA1328, RNase_Y_N
`ENSMUSG00000022556`	Hsf1	Heat shock factor protein 1	2	2	1	29.1	Ref	HMMR_C, HSF_DNA-bind, NPV_P10, Vert_HS_TF, ZapB
`ENSMUSG00000002458`	Rgs19	Regulator of G-protein signaling 19	2	2	1	21.1	Novel	DUF4616, Herpes_LMP1, RGS
`ENSMUSG00000015672`	Mrpl32	Large ribosomal subunit protein bL32m	2	2	1	21.1	Ref	Caa3_CtaG, DUF5347, DZR, NRDD, RecR, Ribosomal_L32p, UPF0547, zf-RING_7, zf-ribbon_3
`ENSMUSG00000029712`	ACTL6B	Actin-like protein 6B	4	2	1	132.2	Novel	Actin, MobA_MobL, MreB_Mbl
`ENSMUSG00000026211`	Obsl1	Obscurin-like protein 1	2	2	1	5.3	Ref	BiPBP_C, C1-set, C2-set_2, DUF6081, Herpes_gE_N, I-set, Ig_2, Ig_3, Ig_4, Ig_5, Ig_6, Ig_C17orf99, Izumo-Ig, Receptor_2B4, V-set, fn3, ig
`ENSMUSG00000039285`	Azi2	5-azacytidine-induced protein 2	2	2	1	18.5	Novel	CENP-F_leu_zip, Cnn_1N, Csm1_N, DMPK_coil, DUF1090, DUF1319, DUF1967, DUF4407, DivIC, FlaC_arch, HIP1_clath_bdg, Herpes_BLRF2, PRKG1_interact, Prefoldin_2, SurA_N, TBD, TMF_TATA_bd, TPR_MLP1_2, UPF0242, YlqD, bZIP_2
`ENSMUSG00000032112`	Trappc4	Trafficking protein particle complex subunit 4	3	2	1	219.4	Mixed	PDZ_6, Sedlin_N, Sybindin
`ENSMUSG00000055692`	Tmem191	Transmembrane protein 191	2	2	1	34.4	Ref	APG6_N, ArsA_ATPase, CheZ, DUF1664, FAM184, GAS, H-kinase_dim, KASH_CCD, Peptidase_M35, TMEM191C
`ENSMUSG00000003208`	Yju2	Splicing factor YJU2	2	2	1	13.2	Ref	IER, Saf4_Yju2, V_ATPase_I
`ENSMUSG00000027333`	Smox	Spermine oxidase	4	2	1	37.0	Mixed	3HCDH_N, AlaDh_PNT_C, Amino_oxidase, DAO, FAD_binding_2, FAD_binding_3, FAD_oxidored, FMO-like, GIDA, Glu_dehyd_C, HI0933_like, Lycopene_cycl, MCRA, MLTD_N, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Shikimate_DH, Thi4, Trp_halogenase
`ENSMUSG00000005417`	Mprip	Myosin phosphatase Rho-interacting protein	2	2	1	10.6	Ref	Apolipoprotein, Baculo_PEP_C, Brr6_like_C_C, DUF3015, DUF6507, FAM184, OmpH, PH, PH_11, RICH, zf-PARP
`ENSMUSG00000026096`	Osgepl1	tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial	2	2	1	13.2	Ref	BcrAD_BadFG, CheC, DUF3659, NADH_4Fe-4S, TsaD
`ENSMUSG00000021007`	Spata7	Spermatogenesis-associated protein 7 homolog	2	2	1	5.3	Ref	APG5, HSD3
`ENSMUSG00000073435`	Nme3	Nucleoside diphosphate kinase 3	2	2	1	42.3	Ref	Amidohydro_2, NDK, PSI_PsaJ, Peptidase_S77
`ENSMUSG00000022159`	Rab2b	Ras-related protein Rab-2B	2	2	1	21.1	Ref	AAA_22, Arf, DAP3, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, TetR_C_16
`ENSMUSG00000035521`	Gnptg	N-acetylglucosamine-1-phosphotransferase subunit gamma	6	2	1	137.5	Mixed	DPM3, PRKCSH, PRKCSH_1
`ENSMUSG00000056851`	Pcbp2	Poly(rC)-binding protein 2	3	2	1	87.2	Ref	KH_1, KH_2, KH_4, KH_5, MOEP19
`ENSMUSG00000021134`	Srsf5	Serine/arginine-rich splicing factor 5	4	2	1	187.7	Ref	DbpA, Nup35_RRM_2, RRM_1, RRM_3, RRM_5, RRM_occluded
`ENSMUSG00000050711`	Scg2	Secretogranin-2	2	2	1	47.6	Ref	Granin, IF2_N, eIF3_N
`ENSMUSG00000018395`	Kif3a	Kinesin-like protein KIF3A	2	2	1	126.9	Novel	AAA_22, AAA_30, DUF1492, DUF4407, Kinesin, Kinesin_assoc, Microtub_bd, PIF1
`ENSMUSG00000054863`	TAFA5	Chemokine-like protein TAFA-5	2	2	1	7.9	Ref	BSP_II, CDC45, CENP-B_dimeris, DUF4637, PRR20, Radial_spoke, TAFA, WRNPLPNID
`ENSMUSG00000001999`	Blvra	Biliverdin reductase A	2	2	1	119.0	Novel	Biliv-reduc_cat, GFO_IDH_MocA, HYPK_UBA, NAD_binding_3, SAM_KSR1
`ENSMUSG00000056121`	Fez2	Fasciculation and elongation protein zeta-2	2	2	1	34.4	Ref	DUF5102, FEZ, FapA, Gluconate_2-dh3, MRP-L46, NPV_P10, V_ATPase_I
`ENSMUSG00000030083`	Abtb1	Ankyrin repeat and BTB/POZ domain-containing protein 1	2	2	1	29.1	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, BACK, BTB
`ENSMUSG00000032172`	OLFM2	Noelin-2	3	2	1	166.5	Mixed	ABC_tran_CTD, CLZ, DUF1664, Helo_like_N, Matrilin_ccoil, Noelin-1, OLF
`ENSMUSG00000071655`	Ubxn1	UBX domain-containing protein 1	3	2	1	325.2	Ref	CDC45, Presenilin, Selenoprotein_S, UBA, UBX, eIF3_subunit
`ENSMUSG00000035228`	Ccdc106	Coiled-coil domain-containing protein 106	3	2	1	31.7	Novel	CCDC106, SOGA
`ENSMUSG00000071644`	Eef1g	Elongation factor 1-gamma	2	2	1	79.3	Novel	DUF4616, EF1G, GST_C, GST_C_2, GST_C_3, GST_N, GST_N_2, GST_N_3
`ENSMUSG00000000861`	Bcl11a	BCL11 transcription factor A	2	2	1	31.7	Ref	Astro_capsid_p, BSP_II, C1_4, DDHD, DNA_pol_phi, DUF2256, DUF3268, DUF4820, FAM32A, FYVE, Importin_rep_6, NOA36, Nop14, PPP4R2, RNA_pol_3_Rpc31, Ribosomal_60s, TUTase, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-H2C2_2, zf-H2C2_5, zf-HIT, zf_UBZ
`ENSMUSG00000004980`	HNRNPA2B1	Heterogeneous nuclear ribonucleoproteins A2/B1	3	2	1	21.1	Mixed	HnRNPA1, OB_RNB, RRM_1, RRM_7
`ENSMUSG00000027091`	Zc3h15	Zinc finger CCCH domain-containing protein 15	2	2	1	179.8	Ref	DFRP_C, Torus, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf-CCCH_4, zf_CCCH_4
`ENSMUSG00000053453`	Thoc7	THO complex subunit 7 homolog	3	2	1	137.5	Novel	AATF-Che1, COG2, Com_YlbF, DASH_Dad2, DUF1664, DUF4407, EcoEI_R_C, FapA, Med9, THOC7, Tup_N, UPF0193, V_ATPase_I, ubiquitin
`ENSMUSG00000030750`	Nsmce1	Non-structural maintenance of chromosomes element 1 homolog	2	2	1	84.6	Novel	AbiJ_NTD3, C1_1, DZR_2, SMC_Nse1, TMEMspv1-c74-12, TraH, zf-C3HC4, zf-C3HC4_2, zf-RING-like
`ENSMUSG00000038302`	Afg1l	AFG1-like ATPase	2	2	1	2.6	Novel	AAA, AAA_16, AAA_22, AFG1_ATPase, Bac_DnaA, DUF2075, ResIII, UvrD-helicase
`ENSMUSG00000075467`	Dnlz	DNL-type zinc finger protein	2	2	1	5.3	Ref	DUF6112, DUF6510, HypA, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-CSL, zf-DNL
`ENSMUSG00000025781`	Atp5f1c	ATP synthase F(1) complex subunit gamma, mitochondrial	3	2	1	1,985.3	Ref	ACT_4, ATP-synt
`ENSMUSG00000024962`	Vegfb	Vascular endothelial growth factor B	3	2	1	166.5	Mixed	CXCXC, PDGF, VEGF_C
`ENSMUSG00000038244`	Mical2	[F-actin]-monooxygenase MICAL2	2	2	1	44.9	Ref	AlaDh_PNT_C, DAO, FAD_binding_2, FAD_binding_3, FAD_oxidored, GCS, GIDA, HI0933_like, LIM, Lycopene_cycl, NAD_binding_7, NAD_binding_8, Pyr_redox, Pyr_redox_2, Pyr_redox_3, TNFR_c6, Thi4, ThiF, zf-C3HC4_2, zf-RRN7
`ENSMUSG00000079557`	Marchf2	E3 ubiquitin-protein ligase MARCHF2	4	2	1	119.0	Mixed	Baculo_IE-1, RINGv, Vpu, zf-C3HC4, zf-RING_2, zf-rbx1
`ENSMUSG00000005699`	Pard6a	Partitioning defective 6 homolog alpha	5	2	1	81.9	Novel	PB1, PDZ, PDZ_6
`ENSMUSG00000005483`	Dnajb1	DnaJ homolog subfamily B member 1	2	2	1	13.2	Ref	DnaJ, DnaJ_C
`ENSMUSG00000007891`	Ctsd	Cathepsin D	2	2	1	745.5	Novel	A1_Propeptide, Asp, GCIP, TAXi_C, TAXi_N
`ENSMUSG00000034867`	Ankrd27	Ankyrin repeat domain-containing protein 27	2	2	1	5.3	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, DUF6660, EcoEI_R_C, IDO, PEGA, Ribonuc_red_sm, Sec8_exocyst, VPS9
`ENSMUSG00000008167`	FBXW9	F-box/WD repeat-containing protein 9	2	2	1	15.9	Ref	F-box, F-box-like, PQQ_3, WD40, WD40_2, WD40_like
`ENSMUSG00000032120`	C2cd2l	Phospholipid transfer protein C2CD2L	2	2	1	52.9	Ref	C2, PetN, SMP_C2CD2L
`ENSMUSG00000020111`	Micu1	Calcium uptake protein 1, mitochondrial	3	2	1	34.4	Novel	Acyl-thio_N, DUF6559, Dockerin_1, EF-hand_1, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8
`ENSMUSG00000021916`	Glt8d1	Glycosyltransferase 8 domain-containing protein 1	2	2	1	7.9	Ref	Glyco_transf_24, Glyco_transf_8, Trehalose_recp
`ENSMUSG00000076439`	Mog	Myelin-oligodendrocyte glycoprotein	2	2	1	208.8	Novel	Adeno_E4, I-set, Ig_2, Ig_3, Longin, V-set, ig
`ENSMUSG00000032503`	Arpp21	cAMP-regulated phosphoprotein 21	4	2	1	92.5	Ref	Birna_VP5, PIRT
`ENSMUSG00000027953`	Slc50a1	Sugar transporter SWEET1	3	2	1	103.1	Novel	MtN3_slv, PQ-loop, YhhN
`ENSMUSG00000056486`	Chn1	N-chimaerin	2	2	1	719.0	Novel	C1_1, FYVE_2, PHD, PHD_2, RhoGAP, zf-RING-like, zf-RING_9
`ENSMUSG00000006392`	Med8	Mediator of RNA polymerase II transcription subunit 8	2	2	1	81.9	Ref	Baculo_PEP_C, CENP-Q, CSN4_RPN5_eIF3a, Med8, SlyX
`ENSMUSG00000031860`	Pbx4	Pre-B-cell leukemia transcription factor 4	2	2	1	31.7	Novel	CENP-B_N, Carbpep_Y_N, DUF1479, HTH_3, Homeobox_KN, Homeodomain, ORC3_N, PBC, YdaS_antitoxin
`ENSMUSG00000033916`	Chmp2a	Charged multivesicular body protein 2a	3	2	1	378.0	Ref	FapA, Snf7, YlqD
`ENSMUSG00000040390`	Map3k10	Mitogen-activated protein kinase kinase kinase 10	2	2	1	21.1	Novel	ABC1, Epsilon_antitox, OmpH, PEARLI-4, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, RNase_Y_N, SH3_1, SH3_2, SH3_9
`ENSMUSG00000027962`	Vcam1	Vascular cell adhesion protein 1	2	2	1	0.0	Ref	Adhes-Ig_like, C1-set, C2-set, C2-set_2, DUF2808, I-set, ICAM_N, Ig_2, Ig_3, Ig_4, Ig_5, Ig_6, Izumo-Ig, Receptor_2B4, V-set, ig
`ENSMUSG00000029054`	Gabrd	Gamma-aminobutyric acid receptor subunit delta	3	2	1	103.1	Novel	Neur_chan_LBD, Neur_chan_memb
`ENSMUSG00000052214`	Opa3	Optic atrophy 3 protein homolog	2	2	1	31.7	Ref	Band_7, Cep57_MT_bd, DUF2730, DUF6779, DnaG_DnaB_bind, FUSC, MgtE_N, OPA3
`ENSMUSG00000035958`	Tdp2	Tyrosyl-DNA phosphodiesterase 2	2	2	1	7.9	Ref	Exo_endo_phos, UBA_4
`ENSMUSG00000025137`	Pcyt2	Ethanolamine-phosphate cytidylyltransferase	2	2	1	119.0	Novel	CTP_transf_like, DUF218, FAD_syn, Oxidored_molyb, ScdA_N
`ENSMUSG00000002395`	Use1	Vesicle transport protein USE1	3	2	1	163.9	Novel	Adhes-Ig_like, Amnionless, CCB2_CCB4, Collectrin, DUF5383, MCU, MRP-L28, Orf78, Use1
`ENSMUSG00000040383`	Aqr	RNA helicase aquarius	2	2	1	7.9	Ref	AAA, AAA_11, AAA_12, AAA_16, AAA_19, AAA_22, AAA_30, Aquarius_N, DEAD, DUF2075, DUF4240, DnaB_C, GT-D, Parvo_NS1, PhoH, RecA, ResIII, UvrD-helicase, UvrD_C_2
`ENSMUSG00000029433`	Diablo	Diablo IAP-binding mitochondrial protein	2	2	1	34.4	Ref	Hc1, MAGE, Smac_DIABLO
`ENSMUSG00000053119`	Chmp3	Charged multivesicular body protein 3	2	2	1	76.7	Novel	DUF3915, Mcm10, PSD4, SPOUT_MTase, Snf7, zf-ZPR1
`ENSMUSG00000002741`	Ykt6	Synaptobrevin homolog YKT6	2	2	1	34.4	Novel	Longin, Synaptobrevin
`ENSMUSG00000028420`	Tmem38b	Trimeric intracellular cation channel type B	2	2	1	5.3	Ref	Gly_transporter, TRIC, WDCP
`ENSMUSG00000032356`	Rasgrf1	Ras-specific guanine nucleotide-releasing factor 1	2	2	1	134.8	Ref	AAA_11, DUF3877, IQ, PH, PH_11, PH_13, PH_17, PH_20, RasGEF, RasGEF_N, RhoGEF
`ENSMUSG00000033029`	—	UPF0193 protein EVG1 homolog	2	2	1	7.9	Ref	AAA_23, UPF0193
`ENSMUSG00000051855`	Mest	Mesoderm-specific transcript protein	2	2	1	21.1	Ref	Abhydrolase_1, Abhydrolase_6, DUF1057, Hydrolase_4, REV
`ENSMUSG00000026816`	Gtf3c5	General transcription factor 3C polypeptide 5	2	2	1	7.9	Ref	DUF4642, FAM176, Tau95, Tau95_N
`ENSMUSG00000040289`	Hey1	Hairy/enhancer-of-split related with YRPW motif protein 1	2	2	1	81.9	Novel	HLH, HTH_Crp_2, Hairy_orange, dCache_2
`ENSMUSG00000034361`	Cpne2	Copine-2	2	2	1	5.3	Ref	C2, Copine, VMAP-M1, vWA-TerF-like
`ENSMUSG00000027487`	Cdk5rap1	Mitochondrial tRNA methylthiotransferase CDK5RAP1	2	2	1	2.6	Ref	Radical_SAM, TRAM, UPF0004
`ENSMUSG00000049744`	Arhgap15	Rho GTPase-activating protein 15	2	2	1	29.1	Novel	Hexokinase_2, PH, PH_9, RhoGAP
`ENSMUSG00000022856`	Tmem41a	Transmembrane protein 41A	2	2	1	84.6	Ref	SNARE_assoc, ThiW
`ENSMUSG00000015290`	Ubl4a	Ubiquitin-like protein 4A	2	2	1	60.8	Ref	Dsc3_N, Rad60-SLD, TBK1_ULD, Tugs, ubiquitin
`ENSMUSG00000041912`	Tdrkh	Tudor and KH domain-containing protein	2	2	1	13.2	Ref	KH_1, KH_2, KH_4, KH_5, MOEP19, Ribosomal_S12, TUDOR
`ENSMUSG00000069255`	Dusp22	Dual specificity protein phosphatase 22	2	2	1	18.5	Ref	DSPc, EZH2_N, Init_tRNA_PT, PTPlike_phytase, Y_phosphatase
`ENSMUSG00000022151`	Ttc33	Tetratricopeptide repeat protein 33	3	2	1	89.9	Ref	ATG14, ChAPs, TPR_1, TPR_11, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_9
`ENSMUSG00000031708`	Tecr	Very-long-chain enoyl-CoA reductase	4	2	1	2,215.3	Ref	Steroid_dh
`ENSMUSG00000003421`	Nosip	Nitric oxide synthase-interacting protein	2	2	1	63.4	Ref	PWI, Rtf2, U-box, zf-NOSIP, zf-Nse
`ENSMUSG00000021147`	Wdr37	WD repeat-containing protein 37	2	2	1	13.2	Ref	ANAPC4_WD40, CCDC90-like, DivIVA, FAM76, HALZ, IZUMO, NBCH_WD40, Nup160, WD40, YabA
`ENSMUSG00000042078`	Svop	Synaptic vesicle 2-related protein	2	2	1	47.6	Ref	DUF1673, MFS_1, MFS_2, Sugar_tr
`ENSMUSG00000021910`	Nisch	Nischarin	2	2	1	29.1	Ref	LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, PX
`ENSMUSG00000018567`	GABARAP	Gamma-aminobutyric acid receptor-associated protein	2	2	1	816.9	Ref	APG12, ATG8, DUF6501, PRIMA1
`ENSMUSG00000018189`	Uchl5	Ubiquitin carboxyl-terminal hydrolase isozyme L5	3	2	1	37.0	Mixed	BBP1_C, DUF2785, PNPase, Peptidase_C12, UCH_C
`ENSMUSG00000044811`	Cd300c2	CMRF-35-like molecule 4	2	2	1	15.9	Ref	Apq12, Ig_3, V-set, ig
`ENSMUSG00000061086`	Myl4	Myosin light chain 4	2	2	1	119.0	Novel	EF-hand_1, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, HTH_20
`ENSMUSG00000041459`	Tardbp	TAR DNA-binding protein 43	2	2	1	15.9	Ref	Nup35_RRM_2, RL, RRM_1, RRM_3, RRM_5, RRM_7, TDP43_N, Trypsin
`ENSMUSG00000015023`	Ddx19a	ATP-dependent RNA helicase DDX19A	2	2	1	15.9	Ref	DEAD, DUF1811, Helicase_C, ResIII
`ENSMUSG00000038876`	Echdc1	Ethylmalonyl-CoA decarboxylase	3	2	1	55.5	Ref	ECH_1, ECH_2, Prok-RING_4, Sp-DndD, WWE, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000002329`	Mdp1	Magnesium-dependent phosphatase 1	2	2	2	126.9	Ref	ABM, Acid_PPase, HAD_2, Hydrolase, NIF
`ENSMUSG00000024227`	—	—	2	2	2	5.3	Novel	GAP1-M, IQ_SEC7_PH, PH, PH_11, PH_13, PH_16, PH_8, PH_9
`ENSMUSG00000044221`	Grsf1	G-rich sequence factor 1	2	2	4	29.1	Novel	RRM_1, zf-C2H2_assoc
`ENSMUSG00000027574`	Nkain4	Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4	2	2	4	174.5	Ref	12TM_1, DUF1003, NKAIN
`ENSMUSG00000032966`	Fkbp1a	Peptidyl-prolyl cis-trans isomerase FKBP1A	2	2	4	1,874.3	Ref	FKBP_C, Glyoxalase_4
`ENSMUSG00000028634`	—	—	2	2	4	76.7	Novel	Activin_recp, SIP1
`ENSMUSG00000026027`	Stradb	STE20-related kinase adapter protein beta	2	2	4	26.4	Ref	DUF1824, ISET-FN3_linker, Kinase-like, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000024792`	Zfpl1	Zinc finger protein-like 1	2	2	4	66.1	Ref	PHD, PHD_4, zf-4CXXC_R1, zf-B_box, zf-RING_16, zf-RING_2
`ENSMUSG00000036225`	KCTD1	BTB/POZ domain-containing protein KCTD1	2	2	4	34.4	Ref	BTB_2, DUF3504, Phage_int_SAM_1
`ENSMUSG00000039536`	Stau1	Double-stranded RNA-binding protein Staufen homolog 1	3	2	4	42.3	Ref	DND1_DSRM, Staufen_C, dsrm
`ENSMUSG00000026424`	Gpr37l1	G-protein coupled receptor 37-like 1	2	2	4	341.0	Novel	7tm_1, Prominin
`ENSMUSG00000034429`	ZNF707	Zinc finger protein 707	2	2	4	44.9	Ref	BolA, C1_4, CpXC, DpnI, HypA, KRAB, Zn-ribbon_8, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_jaz, zf-H2C2_2, zf-LYAR, zf-RING_7, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000055430`	Nap1l5	Nucleosome assembly protein 1-like 5	2	2	4	430.9	Novel	ANAPC15, Ax_dynein_light, Dicty_REP, HrpB7, Mst1_SARAH, NAP, OmpH, Phage_Mu_Gam, Voldacs
`ENSMUSG00000020832`	Eral1	GTPase Era, mitochondrial	2	2	4	21.1	Ref	AAA_16, AAA_28, AIG1, Arf, Dynamin_N, FeoB_N, G-alpha, GTP_EFTU, KH_1, KH_2, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB, TniB, cobW
`ENSMUSG00000021606`	Ndufs6	NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial	2	2	4	214.1	Ref	Chitin_synth_2, Thiolase_C, zf-CHCC
`ENSMUSG00000020738`	SUMO2	Small ubiquitin-related modifier 2	2	2	4	1,562.3	Ref	DUF5010_C, Rad60-SLD, ubiquitin
`ENSMUSG00000074874`	Ctla2a	Protein CTLA-2-alpha	2	2	4	7.9	Ref	GST_N_5, Inhibitor_I29, Mem_trans
`ENSMUSG00000036372`	TMEM258	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258	2	2	4	140.1	Novel	DUF6512, Ilm1, Ost5, Phage_holin_3_3
`ENSMUSG00000041889`	Shisa4	Protein shisa-4	3	2	4	227.3	Mixed	Shisa
`ENSMUSG00000031585`	Gtf2e2	General transcription factor IIE subunit 2	2	2	4	39.7	Ref	PhoQ_Sensor, TFA2_Winged_2, TFIIE_beta
`ENSMUSG00000021276`	Cinp	Cyclin-dependent kinase 2-interacting protein	2	2	4	63.4	Ref	DUF5089, HAUS-augmin3, Tmemb_cc2, WDCP
`ENSMUSG00000028431`	Elp1	Elongator complex protein 1	2	2	4	29.1	Ref	ANAPC4_WD40, CSF-1, IKI3
`ENSMUSG00000015222`	Map2	Microtubule-associated protein 2	2	2	4	15.9	Novel	MAP2_projctn, RII_binding_1
`ENSMUSG00000031728`	Znf821	Zinc finger protein 821	2	2	4	10.6	Ref	CDC45, CPSF73-100_C, DUF5679, DUF5810, DUF6011, DZR, HMBD, Rad50_zn_hook, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_9, zf-C2H2_jaz, zf-Di19, zf-H2C2_2, zf-HYPF, zf-met
`ENSMUSG00000021087`	rtn1-a	Reticulon-1-A	2	2	4	2,030.2	Novel	DUF4736, Reticulon
`ENSMUSG00000028653`	Trit1	tRNA dimethylallyltransferase	2	2	4	7.9	Ref	AAA, AAA_16, AAA_18, AAA_22, AAA_23, AAA_25, AAA_28, AAA_33, AAA_5, ABC_tran, Cytidylate_kin, DUF87, Hpr_kinase_C, IPPT, IPT, KTI12, LZ3wCH, Mst1_SARAH, OrsD, PRK, RNA_helicase, zf-C2H2_jaz, zf-met
`ENSMUSG00000004892`	Bcan	Brevican core protein	2	2	4	134.8	Novel	EGF, EGF_2, EGF_3, EGF_CA, I-set, Ig_2, Ig_3, Izumo-Ig, Laminin_EGF, Lectin_C, Sushi, V-set, Xlink, hEGF, ig
`ENSMUSG00000030122`	Ptms	Parathymosin	2	2	4	55.5	Novel	CCSAP, CDC27, FAM60A, Myc_N, Nop14, Pox_Ag35, Prothymosin, RR_TM4-6, RXT2_N, Rhabdo_ncap, Sec16, Sporozoite_P67, Vfa1
`ENSMUSG00000024146`	Cript	Cysteine-rich PDZ-binding protein	2	2	4	301.4	Ref	Cas_CXXC_CXXC, Cript, DUF1272, DUF2039, DZR_2, Fin, Gin, PLATZ, Rhodanese_C, zf-RING_9, zinc_ribbon_2
`ENSMUSG00000068921`	Dap3	Small ribosomal subunit protein mS29	2	2	4	13.2	Ref	AAA_25, DAP3, DUF1841, DUF3944, DUF4688
`ENSMUSG00000092607`	Scnm1	Sodium channel modifier 1	2	2	4	21.1	Ref	SCNM1_acidic, zf-SCNM1
`ENSMUSG00000022536`	Glyr1	Cytokine-like nuclear factor N-PAC	2	2	4	7.9	Ref	2-Hacid_dh_C, 3HCDH_N, AT_hook, ApbA, CholecysA-Rec_N, F420_oxidored, NAD_Gly3P_dh_N, NAD_binding_11, NAD_binding_2, PWWP, Sacchrp_dh_NADP, Shikimate_DH
`ENSMUSG00000058297`	Spock2	Testican-2	2	2	4	282.9	Ref	EF-hand_6, EF-hand_7, Kazal_1, Kazal_2, SPARC_Ca_bdg, Thyroglobulin_1
`ENSMUSG00000031388`	Naa10	N-alpha-acetyltransferase 10	3	2	4	161.3	Ref	Acetyltransf_1, Acetyltransf_10, Acetyltransf_13, Acetyltransf_15, Acetyltransf_3, Acetyltransf_7, Acetyltransf_9, FR47
`ENSMUSG00000025041`	Nt5c2	Cytosolic purine 5'-nucleotidase	2	2	4	2.6	Ref	5_nucleotid, BCAS2, DUF2095, Filament, Filament_head, bZIP_2
`ENSMUSG00000025735`	Rhbdl1	Rhomboid-related protein 1	2	2	4	74.0	Novel	DUF5580, HPP, Rhomboid
`ENSMUSG00000027316`	Gfra4	GDNF family receptor alpha-4	6	2	4	50.2	Mixed	GDNF, MmgE_PrpD
`ENSMUSG00000069049`	Eif2s3y	Eukaryotic translation initiation factor 2 subunit 3, Y-linked	2	2	4	23.8	Novel	Dynamin_N, G-alpha, GTP_EFTU, GTP_EFTU_D2, MMR_HSR1, MMR_HSR1_Xtn, RsgA_GTPase, eIF2_C
`ENSMUSG00000024234`	Mtpap	Poly(A) RNA polymerase, mitochondrial	3	2	4	31.7	Ref	Nrap_D2, PAP_assoc, RL, tRNA_NucTransf2
`ENSMUSG00000064181`	Rab3ip	Rab-3A-interacting protein	2	2	4	29.1	Ref	ATG16, Borrelia_P83, Crescentin, DUF5082, FAM76, FapA, HMMR_N, Sec2p, TMF_TATA_bd, TPR_MLP1_2, UPF0242
`ENSMUSG00000040462`	Os9	Protein OS-9	2	2	4	111.0	Ref	PRKCSH, PRKCSH_1, RRN3, SNAP-25
`ENSMUSG00000034194`	R3hcc1	R3H and coiled-coil domain-containing protein 1	2	2	4	29.1	Ref	BUD22, CSRNP_N, DUF4407, Dicty_REP, NCBP3, R3H, SDA1
`ENSMUSG00000028759`	Hp1bp3	Heterochromatin protein 1-binding protein 3	2	2	4	15.9	Ref	CCD97-like_C, EMC7_beta-sandw, Linker_histone, Nop14, RRN3, SDA1, SURF2, YL1
`ENSMUSG00000057113`	Npm1	Nucleophosmin	2	2	4	81.9	Ref	CDC45, NOA36, NPM1-C, Nop14, Nucleoplasmin, SDA1, TFIIF_alpha, YL1
`ENSMUSG00000026273`	Mterf4	Transcription termination factor 4, mitochondrial	3	2	4	47.6	Ref	mTERF
`ENSMUSG00000023723`	Mrps23	Small ribosomal subunit protein mS23	3	2	4	177.1	Ref	DUF2573, MRP-S23, MRP-S25
`ENSMUSG00000058833`	Rex1bd	Required for excision 1-B domain-containing protein	2	2	4	230.0	Ref	DNA_repr_REX1B, EST1, FliJ, MscS_porin
`ENSMUSG00000039771`	POLR2J	DNA-directed RNA polymerase II subunit RPB11	2	2	4	132.2	Ref	RNA_pol_L, RNA_pol_L_2, SHNi-TPR
`ENSMUSG00000003411`	Rab3b	Ras-related protein Rab-3B	3	2	4	44.9	Ref	AAA_16, AAA_24, Arf, FeoB_N, Fin, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB

GO Term Enrichment (QC-filtered domain variant genes, FDR < 0.05)

Overrepresented GO terms in genes with genuine domain architecture variants (after artifact removal). These terms indicate which biological processes, molecular functions, and cellular compartments are enriched for alternative splicing that alters protein domain composition.

All Significant GO Terms

Namespace	GO ID	Term	DV Genes	Background	FDR	Genes
BP	`GO:0006886`	intracellular protein transport	28/608	126/6867	`0.028639`	ARF1, Acd, Ap3m2, Arfip2, Aspscr1, Bcap31, CLTA, Cd74, Cltb, Cog7, Copg1, Exoc6, Klc1, Psap, RAB24, Rab24, Rab28, Ramp2, Rims2, STXBP1, Slu7, Snapin, Snx17, Snx2, Stx5, Stx8, Tmed5, Vti1b

DV Enrichment by Expression Level (CPM from upstream transcriptomics)

Baseline DV Rate

7.7%

617 / 7,970 genes

Peak DV Rate

12.9%

High (50-200) bin

Peak Enrichment

1.7×

vs baseline

Chi-square trend

p < 0.001

Expression Bin	Total Genes	DV Genes	DV %	Fold vs Baseline
Silent (<1) CPM	534	9	1.7%	0.2×
Low (1-10) CPM	2,210	68	3.1%	0.4×
Moderate (10-50) CPM	2,909	246	8.5%	1.1×
High (50-200) CPM	1,565	202	12.9%	1.7×
Very high (>=200) CPM	752	92	12.2%	1.6×

Bar height shows the percentage of genes that are domain-variant within each expression bin. Dashed line = overall DV rate (7.7%). If DV enrichment increases monotonically with expression, ascertainment bias (more reads = more isoform discovery) is the dominant driver. Enrichment above baseline at specific expression tiers may indicate biological enrichment beyond the detection effect. CPM from 378,281 mRNA reads (0.1% rRNA excluded).

ORF Type Distribution

NMD Prediction by ORF Type

NMD Candidates by Evidence Tier

NMD Expression Validation (CPM from upstream transcriptomics)

NMD Expression Rate

97.9%

277 / 283

Non-NMD Expression Rate

95.1%

9,762 / 10,261

Fold Difference

1.0×

non-NMD / NMD

Tier 1 NMD Expression

97.2%

106 / 109

NMD Candidates (n=283)

Non-NMD (n=10,261)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual transcript CPM values are overlaid as jittered points. NMD candidates are expressed at 97.9% (median 7.9 CPM) vs 95.1% (median 13.2 CPM) for non-NMD — no evidence of NMD-mediated degradation.

Output Files

blastp.with_title.tsv HPC 1.6 MB gene_functional_summary.tsv HPC 320.3 KB isoform_annotations.tsv HPC 2.3 MB job.yml HPC 608 B pfam.domtblout HPC 23.2 MB results_summary.json HPC 1241.3 KB

Provenance

Execution	Expression quantification summary
Completed	2026-03-01T04:03:10+00:00

RO-Crate 1.1 Workflow RO-Crate 1.0 FAIR

This analysis is packaged as a Research Object Crate with machine-readable provenance and FAIR metadata.

RO-Crate Metadata (JSON-LD)

Show/hide raw JSON-LD

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                }
            ]
        },
        {
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            "@type": "Dataset",
            "name": "ONT Isoform Functional Annotation \u2014 Run #52",
            "description": "Pfam domain search, SwissProt homology, NMD prediction, and functional diversity classification for ONT transcript isoforms.",
            "datePublished": "2026-03-01",
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                "@id": "https://creativecommons.org/licenses/by/4.0/"
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        {
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            "name": "cerebral cortex",
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            "name": "total RNA extract",
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        {
            "@id": "http://purl.obolibrary.org/obo/OBI_0001479",
            "@type": "DefinedTerm",
            "name": "specimen from organism",
            "identifier": "OBI:0001479",
            "termCode": "OBI:0001479"
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        {
            "@id": "http://purl.obolibrary.org/obo/UBERON_0000007",
            "@type": "DefinedTerm",
            "name": "pituitary gland",
            "identifier": "UBERON:0000007",
            "termCode": "UBERON:0000007"
        },
        {
            "@id": "http://purl.obolibrary.org/obo/UBERON_0002107",
            "@type": "DefinedTerm",
            "name": "liver",
            "identifier": "UBERON:0002107",
            "termCode": "UBERON:0002107"
        },
        {
            "@id": "http://purl.obolibrary.org/obo/OBI_0600047",
            "@type": "DefinedTerm",
            "name": "sequencing assay",
            "identifier": "OBI:0600047",
            "termCode": "OBI:0600047"
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        {
            "@id": "http://purl.obolibrary.org/obo/OBI_0000711",
            "@type": "DefinedTerm",
            "name": "library preparation",
            "identifier": "OBI:0000711",
            "termCode": "OBI:0000711"
        },
        {
            "@id": "http://purl.obolibrary.org/obo/OBI_0666667",
            "@type": "DefinedTerm",
            "name": "nucleic acid extraction",
            "identifier": "OBI:0666667",
            "termCode": "OBI:0666667"
        },
        {
            "@id": "http://purl.obolibrary.org/obo/OBI_0000659",
            "@type": "DefinedTerm",
            "name": "specimen collection process",
            "identifier": "OBI:0000659",
            "termCode": "OBI:0000659"
        },
        {
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            "@type": "DefinedTerm",
            "name": "assay",
            "identifier": "OBI:0000070",
            "termCode": "OBI:0000070"
        },
        {
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            "@type": "PropertyValue",
            "name": "strain",
            "value": "C57BL/6J",
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            "@type": "PropertyValue",
            "name": "strain",
            "value": "C57BL/6J",
            "propertyID": "https://www.informatics.jax.org/strain/MGI:3028467"
        },
        {
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            "@type": "PropertyValue",
            "name": "strain",
            "value": "C57BL/6J",
            "propertyID": "https://www.informatics.jax.org/strain/MGI:3028467"
        },
        {
            "@id": "#annotation-267",
            "@type": "PropertyValue",
            "name": "strain",
            "value": "DBA/2J",
            "propertyID": "https://www.informatics.jax.org/strain/MGI:2684695"
        },
        {
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                    "position": 1,
                    "text": "# SOP: Dissection and Collection of Tissue from 10-Week-Old Male Mice\n\n**Applies to:** 10-week-old male mice (C57BL/6J, DBA/2J, or other inbred strains)\n**Tissues collected:** Cortex, pituitary, liver\n**Performed by:** Surgical team, The Jackson Laboratory, Bar Harbor, ME, USA\n**Shipping:** Dry shipper via FedEx International Priority to Australia\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nTo obtain high-quality, snap-frozen tissue samples (cortex, pituitary, liver) from 10-week-old male mice for downstream molecular analysis (RNA extraction, DNA extraction, proteomics). Tissues are collected at The Jackson Laboratory and shipped internationally to Australia under cryogenic conditions to preserve nucleic acid and protein integrity.\n\n## Scope\n\nAll personnel involved in the planning, coordination, dissection, tissue collection, and shipment of mouse tissue samples between The Jackson Laboratory (USA) and the receiving laboratory (Australia).\n\n## Responsibilities\n\n* **Principal Investigator (receiving lab):** Define experimental design, strain requirements, tissue list, and sample labelling scheme. Coordinate import permits and institutional approvals.\n* **Jackson Laboratory surgical team:** Perform euthanasia, dissection, tissue collection, snap-freezing, and packaging for shipment.\n* **Shipping coordinator:** Arrange FedEx International Priority shipment, customs documentation, and dry shipper logistics.\n* **Receiving lab personnel:** Receive shipment, verify sample integrity, transfer to long-term storage, and log receipt.\n\n## Regulatory and Ethical Requirements\n\n* All animal procedures must be approved by The Jackson Laboratory IACUC (Institutional Animal Care and Use Committee) under an active protocol.\n* Receiving institution must hold a valid **import permit** for biological materials from the relevant national authority (e.g., Australian Department of Agriculture, Fisheries and Forestry \u2014 DAFF).\n* Ensure compliance with CITES regulations (not typically applicable for laboratory mice, but verify for specific strains).\n* **Biosecurity:** Frozen tissues from SPF (Specific Pathogen Free) mice. Health monitoring reports from Jackson Laboratory should accompany the shipment.\n\n---\n\n## Materials and Equipment\n\n### At Jackson Laboratory (dissection)\n\n* Certified CO\u2082 euthanasia station or injectable anaesthetic (as per IACUC protocol)\n* Cervical dislocation tools (secondary method of euthanasia)\n* Sterile surgical instruments: fine scissors, forceps (curved and straight), scalpel with #10 and #15 blades, micro-dissecting forceps, bone rongeurs (for skull)\n* Sterile surgical drapes and pads\n* Sterile PBS (phosphate-buffered saline), ice-cold\n* Liquid nitrogen in Dewar flask\n* Pre-labelled cryovials (1.5\u20132.0 mL, cryogenic-rated, screw-cap)\n* Cryogenic labels (resistant to LN\u2082 and \u221280 \u00b0C)\n* RNAlater (optional, as backup for tissues not immediately snap-frozen)\n* Absorbent pads and biohazard waste bags\n* PPE: lab coat, gloves, face shield (for LN\u2082 handling)\n\n### For shipping\n\n* **Dry shipper** (vapour-phase liquid nitrogen dewar, e.g., MVE SC 4/2V or Taylor-Wharton CX100)\n* Cryogenic storage canes or racks\n* Secondary containment bags (leak-proof, absorbent material)\n* FedEx International Priority shipping account\n* Customs documentation: commercial invoice, packing list, import permit, health certificate, AQIS declaration\n* Temperature data logger (optional but recommended)\n\n### At receiving laboratory (Australia)\n\n* \u221280 \u00b0C ultra-low freezer with allocated space\n* Cryogenic gloves and face shield\n* Sample inventory system / database\n\n---\n\n## Procedure\n\n### 1. Pre-Dissection Planning\n\n1. Confirm **mouse strain, sex (male), and age (10 weeks \u00b1 2 days)** with Jackson Laboratory.\n2. Provide Jackson Laboratory with:\n   * Number of animals required (biological replicates)\n   * Tissue list: **cortex, pituitary, liver**\n   * Sample labelling scheme (e.g., strain_tissue_replicate: C57_CTX_1, DBA_PIT_3)\n   * Any special handling instructions (e.g., morning collection to control circadian effects)\n3. Confirm **IACUC protocol** is active and covers the planned procedures.\n4. Obtain **Australian import permit** (DAFF) for frozen animal tissues. Allow 4\u20136 weeks lead time.\n5. Arrange **FedEx International Priority** account and confirm dry shipper availability and return logistics.\n\n### 2. Animal Preparation\n\n1. House mice under standard conditions (12 h light/dark cycle, ad libitum food and water, 20\u201324 \u00b0C) for at least 1 week prior to collection to minimise stress-related transcriptomic changes.\n2. Record for each animal:\n   * Strain and stock number\n   * Date of birth and age at collection\n   * Body weight\n   * Cage number and housing conditions\n   * Any health observations or abnormalities\n3. Fast animals for **4\u20136 hours** prior to collection if liver metabolomic consistency is required (optional \u2014 confirm with PI).\n\n### 3. Euthanasia\n\n1. Euthanise by **CO\u2082 inhalation** (gradual displacement, 30\u201370% chamber volume/min) followed by **cervical dislocation** as secondary confirmation, in accordance with AVMA Guidelines and IACUC protocol.\n2. Confirm death: absence of heartbeat, respiration, and pedal reflex.\n3. Record **time of death** \u2014 proceed to dissection immediately (within 5 min) to minimise RNA degradation.\n\n### 4. Tissue Dissection and Collection\n\nPerform all dissections on a clean, sterile surface. Use separate sterile instruments for each tissue to prevent cross-contamination. Work quickly \u2014 total dissection time per animal should be **<15 minutes**.\n\n#### 4a. Brain \u2014 Cortex\n\n1. Decapitate the mouse. Remove the skin overlying the skull.\n2. Using bone rongeurs or fine scissors, carefully open the skull along the sagittal suture from the foramen magnum to the olfactory bulbs.\n3. Gently lift and peel back the skull plates to expose the brain.\n4. Using curved forceps, carefully lift the brain from the cranial cavity, severing cranial nerves and the spinal cord at the brainstem.\n5. Place the brain dorsal-side up on a cold surface (glass plate on ice or cold PBS-dampened filter paper).\n6. Using a scalpel or razor blade, remove the **cerebral cortex** by making a coronal cut posterior to the olfactory bulbs and peeling the cortical hemispheres away from the underlying structures (hippocampus, striatum, thalamus).\n7. Transfer cortex tissue immediately to a **pre-labelled cryovial**.\n8. **Snap-freeze** by plunging the cryovial into liquid nitrogen. Hold submerged for \u226530 seconds.\n\n#### 4b. Pituitary\n\n1. After removing the brain, examine the base of the skull (sella turcica).\n2. The **pituitary gland** is a small (~1 mm), pinkish structure sitting in the sella turcica.\n3. Using fine-tipped forceps, gently tease the pituitary free from the surrounding membrane and bone.\n4. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen immediately.\n5. **Note:** The pituitary is very small \u2014 take care not to lose or fragment it. If it fragments, collect all pieces.\n\n#### 4c. Liver\n\n1. Open the abdominal cavity with a midline ventral incision from pelvis to sternum.\n2. Retract the intestines to expose the liver.\n3. Identify the **left lateral lobe** (largest, most accessible lobe).\n4. Excise a **~50\u2013100 mg piece** (approximately 5 \u00d7 5 \u00d7 3 mm) from the left lateral lobe using a clean scalpel or razor blade.\n5. Briefly blot on sterile filter paper to remove excess blood.\n6. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen.\n7. If the entire liver is required, excise all lobes, record total liver weight, and snap-freeze in separate vials.\n\n### 5. Post-Dissection\n\n1. Transfer all snap-frozen cryovials from liquid nitrogen to a **\u221280 \u00b0C freezer** within 1 hour of collection.\n2. Dispose of carcasses and biological waste according to Jackson Laboratory institutional procedures.\n3. Clean and decontaminate all instruments and work surfaces.\n4. Complete the **dissection log** for each animal:\n\n   | Field | Record |\n   |---|---|\n   | Animal ID | Strain, stock #, ear tag/toe clip |\n   | Date and time of euthanasia | |\n   | Body weight (g) | |\n   | Tissues collected | Cortex, pituitary, liver |\n   | Tissue appearance | Normal / abnormal (describe) |\n   | Cryovial labels | List all vial IDs |\n   | Operator | Name and signature |\n   | Notes | Any deviations or observations |\n\n### 6. Packaging for International Shipment\n\n1. **Charge the dry shipper** with liquid nitrogen at least 24 hours before packing. Drain excess liquid \u2014 the dry shipper must contain only **vapour-phase nitrogen** (no free liquid) for air transport compliance (IATA).\n2. Verify the dry shipper holds temperature below **\u2212150 \u00b0C** and has a rated hold time sufficient for transit (typically 10\u201314 days).\n3. Place cryovials in **cryogenic canes or racks** and insert into the dry shipper.\n4. Seal the dry shipper and place in the **outer shipping container** (insulated box or hard case).\n5. Include **absorbent material** inside the secondary containment bag (regulatory requirement for biological specimens).\n6. Attach a **temperature data logger** (optional but recommended) to monitor transit conditions.\n7. Prepare shipping documentation:\n   * **Commercial invoice** (contents: frozen mouse tissue samples, no commercial value / research use only)\n   * **Packing list** (number of vials, tissue types, strain, animal IDs)\n   * **Australian import permit** (DAFF-issued)\n   * **Health monitoring report** from Jackson Laboratory (SPF status)\n   * **Dangerous goods declaration** \u2014 Class 9 UN3373 Biological Substance Category B (if required by carrier) or exempt if shipped as non-infectious, non-hazardous research specimens in a dry shipper\n   * **IATA shipper's declaration** for magnetized material (dry shipper contains no free liquid but is classified as a cryogenic vessel)\n\n### 7. Shipment\n\n1. Schedule **FedEx International Priority** pickup from Jackson Laboratory (Bar Harbor, ME, USA).\n2. Ship **Monday\u2013Wednesday** to avoid weekend delays at customs.\n3. Estimated transit time: **3\u20135 business days** (Bar Harbor \u2192 Australian destination).\n4. Provide the receiving lab with the **tracking number** and expected arrival date.\n5. Alert the receiving lab to arrange customs clearance and immediate collection upon arrival.\n\n### 8. Receipt at Australian Laboratory\n\n1. **Collect the shipment immediately** upon arrival \u2014 do not allow it to sit at the depot.\n2. Open the dry shipper and verify:\n   * All cryovials are present and match the packing list\n   * Vials are still frozen (frost visible on vials)\n   * No signs of thawing (condensation, liquid in vials)\n   * Temperature data logger reading (if included) \u2014 confirm temperature remained below \u2212150 \u00b0C throughout transit\n3. Transfer all cryovials immediately to a **\u221280 \u00b0C freezer**.\n4. Log receipt in the laboratory sample inventory:\n\n   | Field | Record |\n   |---|---|\n   | Date received | |\n   | Shipment tracking # | |\n   | Dry shipper condition | Frost present / temperature OK |\n   | Number of vials received | |\n   | Vials match packing list? | Yes / No (note discrepancies) |\n   | Storage location | Freezer ID, shelf, box, position |\n   | Received by | Name and signature |\n\n5. Return the **dry shipper** to Jackson Laboratory per agreed logistics (typically FedEx return shipment).\n\n---\n\n## Quality Control\n\n* **RNA integrity:** Downstream RNA extraction should target RIN/RQN \u2265 7.0 for all tissues. Pituitary may yield lower due to small size \u2014 RQN \u2265 5.0 acceptable.\n* **Dissection time:** Total time from euthanasia to snap-freeze should be **<15 minutes per animal**. Record actual times.\n* **Freeze-thaw:** Tissues must not undergo any freeze-thaw cycles before processing. Monitor freezer temperature logs.\n\n## Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Pituitary fragmented or lost | Record in log; may need to pool from multiple animals or exclude |\n| Tissue partially thawed on arrival | Record in log; process immediately for extraction; note in downstream QC |\n| Customs delay >48 h | Contact FedEx and customs broker; verify dry shipper hold time is sufficient |\n| Dry shipper running low | If hold time is marginal, arrange emergency transfer to local \u221280 \u00b0C |\n| Import permit expired | Do not ship; renew permit before proceeding (allow 4\u20136 weeks) |\n| Animal health abnormality | Record observation; PI to decide whether to include or exclude the animal |\n\n## Waste Disposal\n\n* Mouse carcasses: dispose per Jackson Laboratory IACUC-approved procedures (incineration or licensed biological waste contractor).\n* Sharps (scalpel blades, needles): sharps container.\n* Liquid nitrogen: allow to evaporate in well-ventilated area. Do not pour down drains.\n\n## Records to Maintain\n\n* Approved IACUC protocol number\n* Australian import permit (DAFF) \u2014 copy\n* Dissection logs for each animal\n* Shipping documentation (commercial invoice, packing list, tracking number)\n* Temperature logger data (if used)\n* Sample receipt log\n* Freezer storage map / inventory database entry\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-40",
            "@type": "LabProtocol",
            "name": "Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit",
            "description": "Simultaneous purification of genomic DNA and total RNA from the same tissue sample (\u226430 mg) using selective AllPrep DNA spin columns and RNeasy silica membrane technology. Includes on-column DNase treatment for RNA.",
            "url": {
                "@id": "https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/multianalyte-and-virus/allprep-dna-rna-mini-kit"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "# SOP: Simultaneous DNA and RNA Extraction from Animal Tissues using QIAGEN AllPrep DNA/RNA Mini Kit\n\n**Applies to:** Fresh or frozen animal tissues \u226430 mg per spin column\n**Kit:** QIAGEN AllPrep DNA/RNA Mini Kit (Cat. No. 80204)\n**Location:** __________\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nSimultaneous purification of genomic DNA and total RNA from the same tissue sample using the AllPrep DNA spin column (selective DNA binding in high-salt buffer) and the RNeasy spin column (silica membrane RNA binding). This enables paired DNA/RNA analysis from a single biopsy or tissue aliquot.\n\n## Scope\n\nAll personnel performing dual DNA/RNA extraction from animal tissues in this laboratory.\n\n## Responsibilities\n\n* **Operators:** Follow this SOP, record batch details, and maintain RNase-free practice.\n* **Supervisors:** Ensure training, PPE compliance, and kit maintenance.\n\n## Safety and RNase-Free Practice\n\n* Wear lab coat, gloves, and eye protection.\n* Work in a clean area. Treat benches and tools with RNase decontamination solution.\n* \u03b2-mercaptoethanol is toxic and has a strong odor. Handle in a fume hood.\n* Use RNase-free tubes, tips, and reagents only. Change gloves frequently.\n* Buffer RLT Plus contains guanidine isothiocyanate \u2014 harmful if swallowed or in contact with skin. Do not mix with bleach.\n\n## References\n\n* QIAGEN AllPrep DNA/RNA Mini Handbook, November 2020.\n* NASA GeneLab SOP GL-SOP-3.1 v1.0.\n\n---\n\n## Materials and Equipment\n\n**Provided in kit (Cat. No. 80204, 50 preps)**\n\n* Buffer RLT Plus\n* Buffer RW1\n* Buffer RPE (concentrate \u2014 add ethanol before first use)\n* Buffer AW1\n* Buffer AW2\n* AllPrep DNA Mini Spin Columns\n* RNeasy Mini Spin Columns\n* Collection Tubes (1.5 mL and 2 mL)\n* RNase-free Water\n\n**Not supplied**\n\n* \u03b2-mercaptoethanol (\u03b2-ME)\n* 96\u2013100% ethanol\n* 70% ethanol (freshly prepared)\n* RNase-free DNase Set (Cat. No. 79254) \u2014 for on-column DNase treatment\n* RNase/DNase-free 1.5 mL tubes\n* Microcentrifuge capable of \u226512,000 \u00d7 g\n* Homogenization tools: rotor-stator homogenizer, or TissueLyser with stainless steel beads (3\u20137 mm), or RNase-free pestle, or mortar and pestle with liquid nitrogen\n* Optional: RNase-free syringe with 20-gauge needle\n\n---\n\n## Preparations (before each extraction session)\n\n1. **Prepare Buffer RPE** (first use only)\n   * Add **4 volumes of 96\u2013100% ethanol** to the concentrate. Mark the bottle to indicate ethanol has been added.\n\n2. **Prepare fresh Lysis Buffer**\n   * Add **10 \u00b5L \u03b2-mercaptoethanol per 1 mL Buffer RLT Plus**. Mix well. Prepare only what you need; discard unused buffer.\n\n3. **Prepare DNase Mix** (per sample)\n   * Dissolve lyophilized DNase I (1500 Kunitz units) in **550 \u00b5L** RNase-free water. Mix gently. Aliquot and store at \u221220 \u00b0C (stable up to 9 months; thawed aliquots stable at 2\u20138 \u00b0C for 6 weeks).\n   * Per sample: **10 \u00b5L DNase I stock + 70 \u00b5L Buffer RDD**. Mix by gentle inversion \u2014 **do not vortex**.\n\n4. **Pre-chill microcentrifuge** to 4 \u00b0C if required for lysate clarification.\n\n5. **Heat 2 mL RNase/DNase-free water** to 70 \u00b0C for DNA elution (optional, improves yield).\n\n6. **Input tissue mass**\n\n   | Tissue type | Maximum input |\n   |---|---|\n   | Standard soft tissue | \u226430 mg |\n   | Muscle, heart, skin | \u226415 mg (LN\u2082-preserved) or \u226410 mg (RNAlater) |\n   | Liver | \u226420 mg |\n\n---\n\n## Procedure\n\n### 1. Tissue Disruption and Homogenization\n\n1. Weigh tissue (\u226430 mg). Place in a pre-chilled RNase-free tube on ice.\n2. Add **600 \u00b5L Buffer RLT Plus** (with \u03b2-ME).\n3. Homogenize immediately using one of the following:\n\n   **Option A \u2014 Rotor-stator:**\n   Homogenize for **30\u201345 s** until no visible tissue pieces remain.\n\n   **Option B \u2014 TissueLyser / bead mill:**\n   Add a single **5 mm stainless steel bead**. Run at **25 Hz for 2 \u00d7 2 min** (rotate adapter halfway).\n\n   **Option C \u2014 Mortar & pestle with liquid nitrogen:**\n   Grind tissue to a fine powder under LN\u2082. Transfer powder to a tube, let LN\u2082 evaporate, then add Buffer RLT Plus and vortex.\n\n   **Option D \u2014 Needle and syringe:**\n   Pass lysate through a **20-gauge needle** at least **5\u201310 times** until homogeneous.\n\n4. Centrifuge the lysate at **full speed (\u226512,000 \u00d7 g) for 3 min**, RT.\n5. Carefully transfer the supernatant to a new tube. **Do not disturb the pellet.**\n\n### 2. Bind Genomic DNA\n\n1. Transfer the cleared lysate onto an **AllPrep DNA Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 30 s**, RT.\n3. **Set aside the DNA column** at 4 \u00b0C (or RT for up to 1 h) \u2014 proceed to RNA purification first.\n4. **Save the flow-through** for RNA isolation.\n\n### 3. Adjust RNA Binding Conditions\n\n1. Add **1 volume of freshly prepared 70% ethanol** to the flow-through (e.g. 600 \u00b5L ethanol to 600 \u00b5L flow-through). For **liver tissue**, use **50% ethanol** instead.\n2. Mix well by pipetting. **Do not centrifuge.** A precipitate may form \u2014 this is normal; proceed immediately.\n\n### 4. Bind and Wash RNA\n\n1. Transfer up to **700 \u00b5L** of the sample onto an **RNeasy Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Repeat until all sample has passed through the column.\n4. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 5. On-Column DNase Treatment\n\n1. Pipette **80 \u00b5L DNase Mix** (10 \u00b5L DNase I + 70 \u00b5L Buffer RDD) directly onto the membrane.\n2. Incubate at **RT for 15\u201330 min** (30 min recommended for maximum removal).\n3. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 6. Wash and Dry RNA Column\n\n1. **Replace the collection tube** with a new 2 mL tube.\n2. Add **500 \u00b5L Buffer RPE** (ethanol added). Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add another **500 \u00b5L Buffer RPE**. Centrifuge at **\u22658,000 \u00d7 g for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 7. Elute RNA\n\n1. Place the RNeasy column in a new **1.5 mL collection tube**.\n2. Add **30\u201350 \u00b5L RNase-free water** directly onto the membrane.\n3. Incubate **1\u20135 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** RNase-free water for maximum yield. Pool eluates if the second elution is \u226530% of the first.\n6. Place RNA on ice immediately.\n\n### 8. Wash Genomic DNA Column\n\n1. Retrieve the AllPrep DNA column from step 2.\n2. Add **500 \u00b5L Buffer AW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add **500 \u00b5L Buffer AW2**. Centrifuge at **full speed (\u226514,000 \u00d7 g) for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 9. Elute Genomic DNA\n\n1. Place the AllPrep DNA column in a new **1.5 mL collection tube**.\n2. Add **50\u2013100 \u00b5L RNase/DNase-free water** (pre-warmed to 70 \u00b0C for improved yield) directly onto the membrane.\n3. Incubate **2 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** for maximum yield.\n\n### 10. Quantification and Quality Assessment\n\n**RNA:**\n* Measure concentration by Qubit RNA assay or NanoDrop A260.\n* Record A260/280 (expect \u22651.8) and A260/230 (expect \u22651.5) ratios.\n* Assess integrity by Fragment Analyzer, Bioanalyzer, or TapeStation (target RQN/RIN \u22657 for most applications).\n\n**DNA:**\n* Measure concentration by Qubit dsDNA assay or NanoDrop A260.\n* Record A260/280 (expect ~1.8) and A260/230 ratios.\n* Assess fragment size by TapeStation Genomic DNA assay or agarose gel if required.\n\n### 11. Storage\n\n* **RNA:** Keep on ice for immediate use. Store at **\u221280 \u00b0C** for long term.\n* **DNA:** Store at **\u221220 \u00b0C** (short term) or **\u221280 \u00b0C** (long term). Avoid repeated freeze-thaw cycles.\n\n---\n\n## Special Considerations for Muscle, Heart, and Skin Tissues\n\nThese tissues are dense and fibrous, leading to potential column clogging and lower yields.\n\n* Start with **\u226415 mg** (LN\u2082-preserved) or **\u226410 mg** (RNAlater-preserved).\n* **Pre-warm** Buffers RW1 and RPE to **37 \u00b0C** for 30 min before use.\n* Centrifuge lysate **twice** at full speed to minimize cell debris.\n* Load a maximum of **400 \u00b5L** per column pass.\n* If the column clogs: incubate wash solution on column for **5 min at 37 \u00b0C** before centrifuging.\n* Elute with **pre-warmed (37 \u00b0C) water** in two passes (50 \u00b5L + 30 \u00b5L); combine if second elution is \u226530% of first.\n* If yields remain low: process **two 15 mg aliquots separately** and combine eluates.\n\n---\n\n## Acceptance and Rejection Criteria\n\n* Elution volume and expected yield range documented per tissue type.\n* No visible carryover of debris in eluate.\n* Proceed if spectrophotometric and integrity checks meet project-specific thresholds.\n\n## Expected Yields\n\n| Tissue type | DNA yield | RNA yield |\n|---|---|---|\n| Liver (20 mg) | 10\u201330 \u00b5g | 20\u201360 \u00b5g |\n| Brain (20 mg) | 5\u201315 \u00b5g | 5\u201320 \u00b5g |\n| Kidney (20 mg) | 5\u201315 \u00b5g | 10\u201340 \u00b5g |\n| Heart/Muscle (15 mg) | 2\u201310 \u00b5g | 1\u201310 \u00b5g |\n| Spleen (20 mg) | 15\u201340 \u00b5g | 15\u201360 \u00b5g |\n\n## Troubleshooting\n\n| Problem | Possible cause | Solution |\n|---|---|---|\n| Low RNA yield | Incomplete lysis | Increase homogenization time; ensure \u03b2-ME was added to RLT Plus |\n| Low RNA yield | Column overloaded | Reduce input tissue mass |\n| Low DNA yield | Insufficient lysis | Ensure thorough mechanical disruption before loading AllPrep DNA column |\n| Low DNA yield | DNA column dried out | Do not leave DNA column at RT for >1 h |\n| Genomic DNA in RNA | DNase treatment insufficient | Extend incubation to 30 min; verify DNase I activity |\n| RNA in DNA | Carryover of flow-through | Avoid disturbing interface when transferring lysate |\n| Column clogging | Tissue debris | Pre-clear lysate by additional centrifugation; reduce input mass |\n| Low A260/230 | Guanidine salt carryover | Add an extra RPE/AW2 wash step |\n\n## Waste Disposal\n\n* Collect \u03b2-mercaptoethanol and guanidine-containing wastes according to institutional chemical waste procedures.\n* Dispose of biological materials under local biosafety rules.\n* Spin columns with bound nucleic acids are non-hazardous after elution.\n\n## Records to Maintain\n\n* Date, operator, sample ID, tissue type and mass, homogenization method, reagent lot numbers, elution volume(s), DNA and RNA concentrations, purity ratios, integrity assessment, and storage location.\n* Deviations and corrective actions.\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-37",
            "@type": "LabProtocol",
            "name": "PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)",
            "description": "Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library preparation from total RNA. Uses template-switching reverse transcription followed by PCR amplification with barcoded primers. Compatible with MinION, GridION and PromethION flow cells (R9.4.1). Superseded by SQK-PCS114 in late 2023.",
            "url": {
                "@id": "https://community.nanoporetech.com/docs/prepare/library_prep_protocols/pcr-cdna-barcoding-kit-sqk-pcb111-24"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "## Overview & Kit Contents\n\nThis protocol is based on the Oxford Nanopore Technologies **PCR-cDNA Barcoding Kit (SQK-PCB111.24)**, suitable for generating full-length barcoded cDNA sequencing libraries from total RNA.\n\n### Kit components\n- VNP primer, SSP primer (strand-switching primer)\n- Barcoded PCR primers (BC01\u2013BC24)\n- Rapid Adapter (RAP)\n- Elution Buffer (EB)\n\n### Required reagents (user-supplied)\n- Lambda Exonuclease\n- NEBNext Quick Ligation Reaction Buffer\n- T4 DNA Ligase 2M U/ml\n- 10 mM dNTP solution\n- LongAmp Hot Start Taq 2X Master Mix\n- Maxima H Minus Reverse Transcriptase (200 U/ul) with 5x RT Buffer\n- RNaseOUT, 40 U/ul\n- USER (Uracil-Specific Excision Reagent) Enzyme\n- Exonuclease I\n- AMPure XP beads\n\n### Required equipment\n- Thermal cycler, magnetic rack, Qubit fluorometer, vortex mixer, microcentrifuge"
                },
                {
                    "@type": "HowToStep",
                    "position": 2,
                    "text": "### Input RNA requirements\n\n- **Input**: 1\u201350 ng poly(A)+ mRNA or 50\u2013100 ng total RNA (RIN >= 7 recommended)\n- RNA should be DNase-treated if genomic DNA contamination is suspected\n- Prepare RNA in 7.5 uL nuclease-free water\n- Add 1 uL VNP primer (2 uM) and 1 uL 10 mM dNTPs\n- Incubate at **65 C for 5 min**, then snap-cool on ice for 1 min"
                },
                {
                    "@type": "HowToStep",
                    "position": 3,
                    "text": "### Reverse transcription and strand switching (~90 min)\n\n1. Prepare RT mix on ice (per reaction):\n   - 4 uL 5x RT Buffer\n   - 1 uL RNaseOUT (40 U/uL)\n   - 1 uL nuclease-free water\n   - 2 uL Strand-Switching Primer (SSP, 10 uM)\n   - 1 uL Maxima H Minus RT (200 U/uL)\n2. Add 10 uL RT mix to the 9.5 uL RNA/primer/dNTP mix (total 19.5 uL)\n3. Incubate: **42 C for 90 min** (RT + template switching), **85 C for 5 min** (inactivation), hold 4 C\n4. Add 1 uL RNase H, incubate **37 C for 10 min**\n5. Briefly centrifuge and place on ice"
                },
                {
                    "@type": "HowToStep",
                    "position": 4,
                    "text": "### PCR amplification with barcoded primers (~30 min)\n\n1. Prepare PCR reaction (50 uL total):\n   - 20 uL cDNA from RT step\n   - 25 uL LongAmp Taq 2X Master Mix\n   - 1.5 uL Barcode Primer (BC*XX*, 10 uM)\n   - 3.5 uL nuclease-free water\n2. Cycling conditions:\n   - **95 C, 3 min** (initial denaturation)\n   - **18 cycles**: 95 C 15 s / 62 C 15 s / 65 C 5 min\n   - **65 C, 6 min** (final extension)\n   - Hold at 4 C\n\n**Note**: 18 cycles is suitable for 50-100 ng total RNA input. Reduce to 14-15 cycles for higher inputs."
                },
                {
                    "@type": "HowToStep",
                    "position": 5,
                    "text": "### Bead clean-up and quantification\n\n1. Add 40 uL AMPure XP beads (0.8x ratio) to 50 uL PCR product\n2. Incubate at RT for 5 min\n3. Place on magnetic rack, wait for pellet to form\n4. Wash 2x with 200 uL freshly prepared 80% ethanol\n5. Air-dry pellet for ~30 s (do not over-dry)\n6. Elute in 12 uL EB\n7. Quantify with Qubit dsDNA HS assay\n8. Expected yield: 50-500 ng depending on input RNA quality and quantity"
                },
                {
                    "@type": "HowToStep",
                    "position": 6,
                    "text": "### Pool barcoded libraries and attach Rapid Adapter\n\n1. Pool equimolar amounts of barcoded cDNA libraries (target: 100-200 fmol total)\n2. Adjust volume to 11 uL with EB\n3. Add 1 uL Rapid Adapter (RAP)\n4. Mix gently by flicking, spin down briefly\n5. Incubate at **RT for 5 min**\n6. Library is ready for loading -- **do not vortex** after adapter attachment"
                },
                {
                    "@type": "HowToStep",
                    "position": 7,
                    "text": "### Flow cell priming and loading\n\n1. Prepare priming mix: 30 uL FCT + 1170 uL FCF\n2. Load 800 uL priming mix via priming port, wait 5 min\n3. Prepare loading mix: 12 uL adapted library + 37.5 uL SB + 25.5 uL LB\n4. Load remaining priming mix, then load 75 uL library via SpotON port\n5. Start sequencing in MinKNOW (Kit: SQK-PCB111.24, enable barcode demultiplexing)"
                },
                {
                    "@type": "HowToStep",
                    "position": 8,
                    "text": "## References\n\n- Oxford Nanopore Technologies, **\"PCR-cDNA Barcoding Kit (SQK-PCB111.24)\"**.\n- Earlier version: SQK-PCS111. Kit superseded by SQK-PCS114/SQK-PCB114 (V14 chemistry, R10.4.1 flow cells) in late 2023."
                }
            ]
        },
        {
            "@id": "#protocol-44",
            "@type": "LabProtocol",
            "name": "Fragment Analyzer \u2013 SS NGS Fragment 1-6000 bp",
            "description": "Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-473 kit. Suitable for QC of NGS libraries, PCR products, and size-selected DNA fragments in the 1-6000 bp range.",
            "url": {
                "@id": "https://www.agilent.com/store/en_US/Prod-DNF-473-0500/DNF-473-0500"
            },
            "version": "1.0",
            "category": "measurement",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "**Method:** `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds`\n\n**Instrument:** Agilent/Advanced Analytical Fragment Analyzer (33 cm capillary array)\n\n**Analysis Mode:** NGS\n\n---\n\n## 1. Document Control\n\n* **SOP ID:** FA-NGS-1-6000bp-33cm\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Prepared by:** __________\n* **Approved by:** __________\n* **Review cycle:** 12 months\n\n## 2. Purpose\n\nTo define the procedure for running standard-sensitivity NGS fragment analysis on the Fragment Analyzer using the **DNF-473-33** method. This method resolves DNA fragments in the **1\u20136,000 bp** range with standard sensitivity, suitable for:\n\n* QC of NGS libraries (amplicon, PCR-cDNA, shotgun)\n* Assessment of size selection efficiency (e.g. AMPure bead selection)\n* Verification of library size distribution prior to sequencing\n* Quantification of library concentration\n\n## 3. Scope\n\nApplies to all operators performing NGS library QC or fragment analysis in the 1\u20136,000 bp range using the DNF-473 kit on a Fragment Analyzer.\n\n## 4. Responsibilities\n\n* **Operator:** Execute this SOP, record run metadata, verify QC, and report issues.\n* **Lab lead:** Ensure training, instrument maintenance, and SOP review.\n\n## 5. Safety\n\n* Wear lab coat, safety glasses, and disposable gloves.\n* Handle gels, buffers, and plates per SDS.\n* Avoid contact with high voltage areas during operation. Only open instrument when software indicates safe.\n* Dispose of consumables as per local regulations.\n\n## 6. References\n\n* Fragment Analyzer user manual.\n* DNF-473 SS NGS Fragment 1-6000bp kit instructions.\n* Local laboratory policies for data retention and equipment maintenance.\n\n## 7. Materials and Equipment\n\n**Instrument and configuration**\n\n* Fragment Analyzer with **33 cm** capillary array.\n* Array serial and usage count recorded before each run.\n\n**Reagents and consumables**\n\n* SS NGS Fragment 1-6000bp gel cartridge compatible with `DNF-473-33`.\n* Buffer reservoirs and rinse solution as per kit.\n* 96-well plate(s) and plate seals or caps.\n* DNA ladder (supplied with kit or compatible sizing standard).\n* Nuclease-free water and low-retention tips.\n\n**Software**\n\n* ProSize 3.0 or later for data analysis and PDF report generation.\n\n## 8. Sample Requirements\n\n* DNA libraries in TE, EB, or nuclease-free water.\n* Recommended input: **0.2\u201350 ng/\u00b5L** (standard sensitivity range).\n* Minimum volume per well: as per plate format (typically 2 \u00b5L sample + diluent).\n* Avoid EDTA >5 mM, high salt, or detergents that interfere with electrokinetic injection.\n* For very low concentration libraries (<1 ng/\u00b5L, e.g. post-bead-selection), results may show concentration but no resolved peaks \u2014 this is expected and the TIM value is still informative.\n\n## 9. Pre-Run Checks\n\n1. Inspect capillary array for leaks, kinks, or salt crystallisation. Record **array serial** and **usage count**.\n2. Confirm instrument details in software:\n   * **Device Serial:** record\n   * **FA Version:** record\n3. Verify consumables:\n   * Correct **gel** loaded and **Gel Selection: Gel 1**.\n   * Fresh buffer and rinse trays in correct positions.\n4. Equilibrate reagents to room temperature if required by kit.\n5. Prepare plate map with samples, ladder, controls, and blanks.\n\n## 10. Plate Preparation\n\n1. Thaw and mix reagents gently. Spin down briefly.\n2. Aliquot ladder into designated well (typically position 12 of each row).\n3. Aliquot samples into designated wells per plate map.\n4. Include at least one blank (nuclease-free water) for baseline reference.\n5. Seal the plate. Briefly centrifuge to remove bubbles. Keep sealed until loading.\n\n## 11. Instrument Setup (Software)\n\nSet parameters as follows:\n\n| Parameter | Setting |\n|---|---|\n| Method Name | `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds` |\n| Analysis Mode | `NGS` |\n| Gel Prime | `No` |\n| Full Conditioning | `Yes` (start of day) |\n| Gel Selection | `Gel 1` |\n| Perform Prerun | `5.0 kV` for `30 s` |\n| Rinse | Tray `3`, Row `A`, `# Dips: 2` |\n| Marker 1 | `No` |\n| Sample Injection | `5.0 kV` for `4 s` |\n| Separation | `8.0 kV` for `40.0 min` |\n| # of Capillaries | Per array configuration |\n\n**Note:** The DNF-473 method uses **shorter separation time (40 min)** and **higher separation voltage (8 kV)** compared to the HS Large Fragment method (DNF-464: 60 min, 5 kV), reflecting the smaller target size range.\n\n## 12. Run Procedure\n\n1. Load the sealed sample plate into the instrument.\n2. Confirm tray positions and liquid levels.\n3. In software, confirm the method parameters match Section 11.\n4. Start the run. Observe for the first 2\u20133 minutes for abnormal current, leaks, or errors.\n5. Allow the **40 min** separation to complete.\n\n## 13. Data Analysis (ProSize)\n\n1. Open the completed run in ProSize 3.0.\n2. Verify ladder sizing is correct (expected peaks at kit-specified positions).\n3. For each sample, review:\n   * **Electropherogram** \u2014 peak shape, baseline, and resolution\n   * **Total Concentration (ng/\u00b5L)** \u2014 integrated from all non-marker peaks\n   * **TIM (nmol/L)** \u2014 total integrated molarity\n   * **Main peak size (bp)** \u2014 dominant fragment size\n   * **Number of peaks** \u2014 indicator of library complexity/purity\n4. Export PDF report containing per-sample peak tables, electropherograms, and summary metrics.\n\n## 14. Interpretation Guide\n\n| Observation | Interpretation | Action |\n|---|---|---|\n| Single clean peak at expected size | Library prep and/or size selection successful | Proceed to pooling/sequencing |\n| Broad peak or multiple peaks | Heterogeneous library or incomplete size selection | Consider re-selection or accept depending on application |\n| No peaks but measurable concentration | Library below detection threshold for sizing | Verify by Qubit; may still be usable if concentration is sufficient |\n| Adapter dimer peak (~100-150 bp) | Incomplete cleanup | Re-clean with AMPure beads at appropriate ratio |\n| No signal | Failed library prep, sample degraded, or loading error | Troubleshoot: check sample, re-run, or re-prep |\n\n### Typical Results for ONT PCR-cDNA Libraries\n\n| Library state | Expected concentration | Expected main peak |\n|---|---|---|\n| Pre-size-selection | 4\u201310 ng/\u00b5L | 1,000\u20132,000 bp (broad) |\n| Post-bead-selection (1.5 kb) | 0.5\u20132 ng/\u00b5L | ~1,480 bp (single peak) |\n| Post-bead-selection (4 kb) | 0.5\u20132 ng/\u00b5L | ~3,500\u20134,500 bp (single peak) |\n\n## 15. Quality Control and Acceptance\n\n* Ladder wells exhibit expected profile and sizing.\n* Baseline is stable with distinct peaks where applicable.\n* Migration time consistent across capillaries.\n* If any criterion fails, document, recondition, and repeat controls before analysing unknowns.\n\n## 16. Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Weak or absent signal | Verify injection settings, sample concentration, plate seal integrity, capillary tips in wells |\n| High noise or drifting baseline | Re-condition array, check buffer freshness and tray cleanliness |\n| Inconsistent sizing | Confirm ladder integrity, plate map accuracy, and temperature stability |\n| Frequent current faults | Inspect for bubbles, low liquid levels, or salt contamination; replace consumables |\n\n## 17. Maintenance\n\n* Perform **Full Conditioning** at the start of the day or as required by kit.\n* Replace gels and buffers per shelf-life and use counts.\n* Track **Array Usage Count** at each run and follow vendor guidance for replacement.\n* Clean trays weekly or after spills. Inspect electrodes and stage area.\n\n## 18. Records to Retain\n\nFor each run record:\n\n* Date, time, and operator\n* Device serial and FA software version\n* Array serial and usage count\n* Method name and key parameters\n* Plate map and sample IDs\n* File path to raw data and PDF reports\n* QC outcomes and any deviations\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-35",
            "@type": "LabProtocol",
            "name": "Sequencing Submission",
            "description": "Submit samples for sequencing at the genomics facility. Links kevlab samples to QC app runs after sequencing is complete.",
            "version": "1.0",
            "category": "sequencing"
        },
        {
            "@id": "#measurement-344",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1493",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-345",
            "@type": "PropertyValue",
            "name": "count",
            "value": "3",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-342",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "5.6008",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-343",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "6.1563",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#measurement-348",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1493",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-349",
            "@type": "PropertyValue",
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            "unitText": "count"
        },
        {
            "@id": "#measurement-346",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "6.2061",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-347",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "7.4416",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#measurement-352",
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            "name": "bp",
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            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-353",
            "@type": "PropertyValue",
            "name": "count",
            "value": "3",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-350",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "5.7241",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-351",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "6.8672",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#measurement-356",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1500",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-357",
            "@type": "PropertyValue",
            "name": "count",
            "value": "2",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-354",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "4.2355",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-355",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "4.4695",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#measurement-360",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1485",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-361",
            "@type": "PropertyValue",
            "name": "count",
            "value": "2",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-358",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "4.0452",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-359",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "4.2648",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#measurement-364",
            "@type": "PropertyValue",
            "name": "bp",
            "value": "1485",
            "unitCode": "UO:0000244",
            "unitText": "bp"
        },
        {
            "@id": "#measurement-365",
            "@type": "PropertyValue",
            "name": "count",
            "value": "2",
            "unitCode": "UO:0000189",
            "unitText": "count"
        },
        {
            "@id": "#measurement-362",
            "@type": "PropertyValue",
            "name": "ng/\u00b5L",
            "value": "4.3327",
            "unitCode": "UO:0010050",
            "unitText": "ng/\u00b5L"
        },
        {
            "@id": "#measurement-363",
            "@type": "PropertyValue",
            "name": "nmol/L",
            "value": "4.7107",
            "unitCode": "UO:0000065",
            "unitText": "nmol/L"
        },
        {
            "@id": "#sample-433",
            "@type": "BioSample",
            "name": "1-C57-CORTEX",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001479",
            "organism": "Mus musculus",
            "materialType": "source_material",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-181"
                },
                {
                    "@id": "#annotation-180"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-413"
            }
        },
        {
            "@id": "#sample-435",
            "@type": "BioSample",
            "name": "1-C57-LIVER",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001479",
            "organism": "Mus musculus",
            "materialType": "source_material",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-187"
                },
                {
                    "@id": "#annotation-186"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-413"
            }
        },
        {
            "@id": "#sample-434",
            "@type": "BioSample",
            "name": "1-C57-PITUITARY",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001479",
            "organism": "Mus musculus",
            "materialType": "source_material",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-184"
                },
                {
                    "@id": "#annotation-183"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-413"
            }
        },
        {
            "@id": "#sample-397",
            "@type": "BioSample",
            "name": "1-D",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001051",
            "description": "C57 DNA",
            "organism": "Mus musculus",
            "materialType": "extract",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-217"
                },
                {
                    "@id": "#annotation-216"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-433"
            }
        },
        {
            "@id": "#sample-421",
            "@type": "BioSample",
            "name": "1-DBA-CORTEX",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001479",
            "organism": "Mus musculus",
            "materialType": "source_material",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-145"
                },
                {
                    "@id": "#annotation-144"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-417"
            }
        },
        {
            "@id": "#sample-423",
            "@type": "BioSample",
            "name": "1-DBA-LIVER",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001479",
            "organism": "Mus musculus",
            "materialType": "source_material",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-151"
                },
                {
                    "@id": "#annotation-150"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-417"
            }
        },
        {
            "@id": "#sample-422",
            "@type": "BioSample",
            "name": "1-DBA-PITUITARY",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001479",
            "organism": "Mus musculus",
            "materialType": "source_material",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-148"
                },
                {
                    "@id": "#annotation-147"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-417"
            }
        },
        {
            "@id": "#sample-405",
            "@type": "BioSample",
            "name": "1-R",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0000895",
            "description": "C57 RNA",
            "organism": "Mus musculus",
            "materialType": "extract",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-241"
                },
                {
                    "@id": "#annotation-240"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-433"
            }
        },
        {
            "@id": "#sample-404",
            "@type": "BioSample",
            "name": "10-D",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0001051",
            "description": "DBA DNA",
            "organism": "Mus musculus",
            "materialType": "extract",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-238"
                },
                {
                    "@id": "#annotation-237"
                }
            ],
            "isBasedOn": {
                "@id": "#sample-430"
            }
        },
        {
            "@id": "#sample-412",
            "@type": "BioSample",
            "name": "10-R",
            "additionalType": "http://purl.obolibrary.org/obo/OBI_0000895",
            "description": "DBA RNA",
            "organism": "Mus musculus",
            "materialType": "extract",
            "taxonomicRange": {
                "@id": "http://purl.obolibrary.org/obo/NCBITaxon_10090"
            },
            "additionalProperty": [
                {
                    "@id": "#annotation-262"
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                {
                    "@id": "#sample-421"
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                {
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                {
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                {
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                {
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                "@id": "#protocol-37"
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            "instrument": [
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                {
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                {
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                {
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                {
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                {
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                {
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                {
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                {
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                {
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        {
            "@id": "#process-121",
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                "@id": "#protocol-37"
            },
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            ]
        },
        {
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            "instrument": [
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                    "@id": "#protocol-35"
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                {
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            "category": "sequencing",
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                {
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                {
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}