ONT Isoform Functional Annotation (superseded)

Barcode 24 functional annotation

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Type

CWL

Status

succeeded

Engine

cwltool

Duration

1.7 h

Source Data

Study	Strain-specific cortex gene expression and isoform usage
Sample prep	PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) 2023-05-09
Sequencing	Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) 2023-05-12
Run Data	Run #63 (6 samples)
Samples	C57_rep1_bc10 C57_rep2_bc11 C57_rep3_bc12 DBA_rep1_bc13 DBA_rep2_bc14 DBA_rep3_bc15

Pipeline

ONT Long-Read Transcriptomics (IsoQuant)

Run #46

succeeded

ONT Isoform Functional Annotation (superseded)

Run #53 (this run)

succeeded 1 sources

Combined From

#46 — ONT Long-Read Transcriptomics (IsoQuant) succeeded

Sample Provenance

Process Chain

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

8 samples produced

Dissection and collection of tissue from 10-week-old male mice (C57/BL6J and DBA/2J) continued (2016-10-17) Labbook

Protocol: Dissection and collection of tissue from 10-week-old male mice

24 samples produced

Simultaneous extraction of DNA and RNA from C57 and DBA cortex tissue using Qiagen AllPrep (2016-11-17) Labbook

Protocol: Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit

16 samples produced

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (SQK-PCB111.24) (2023-03-13) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection) (2023-03-14) Labbook

24 measurements across 6 samples

PCR-cDNA barcoding library preparation for C57/DBA mouse RNA (run 3, bc10-15) (2023-05-09) Labbook

Protocol: PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)

6 samples produced

Nanopore PCR-cDNA sequencing of C57/DBA mouse RNA (run 3) (2023-05-12) Labbook

QC Measurements

Fragment Analyzer analysis of C57/DBA PCR-cDNA libraries (pre-selection)

2023-03-14 Labbook

Sample	bp	count	ng/µL	nmol/L
C57_rep1_bc19	1493	3	5.6008	6.1563
C57_rep2_bc20	1493	4	6.2061	7.4416
C57_rep3_bc21	1485	3	5.7241	6.8672
DBA_rep1_bc22	1500	2	4.2355	4.4695
DBA_rep2_bc23	1485	2	4.0452	4.2648
DBA_rep3_bc24	1485	2	4.3327	4.7107

Workflow

ONT Isoform Functional Annotation

#cwl

Software Tools

Tool	Version	URL
cwltool	-	https://github.com/common-workflow-language/cwltool
eldudy007_isoseq:1.0.sif	-	-

Results Summary

Unique Reads

454,809

from upstream transcriptomics

rRNA Reads

304 (0.1%)

mRNA Reads

454,505

used for CPM calculation

Transcripts Annotated

11,042

Pfam Hit Rate

94.4%

SwissProt Hit Rate

95.0%

High-Confidence

8,316 (75.3%)

Tier 1–2

NMD Candidates

289 (2.6%)

Evidence Tier Distribution

Novel Isoform Discovery

Total Isoforms

11,042

Reference

10,083

Novel (IsoQuant)

959

952 with ≥3 reads, 7 low-support

Novel %

8.7%

DV Genes: Novel-Created

266

Variation only from novel isoforms

DV Genes: Novel-Contributed

Novel isoforms add to existing ref variation

DV Genes: Reference-Only

608

Variation from reference annotation alone

Long-read sequencing discovered 959 novel isoforms (8.7%). Of 938 domain variant genes, 266 (28%) show domain architecture variation only because of novel isoforms not present in the reference annotation. In 5 DV genes, only novel isoforms produced detectable ORFs.

Isoform Read Support (CPM from upstream transcriptomics)

Novel Expression Rate

100.0%

959 / 959 (952 with ≥3 reads)

Reference Expression Rate

94.8%

9,561 / 10,083

Novel Median CPM

13.2

median 6 reads (mean 12.1, range 0–605)

Ref Median CPM

11.0

Novel (n=959)

Reference (n=10,083)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual isoform CPM values are overlaid as jittered points. Log₁₀ scale; dashed line marks CPM = 1 expression threshold. Per-model CPM from OUT.transcript_model_counts.tsv

Novel Isoform Functional Impact

Domain Altered

105

10.9% of novel

NMD Candidates

7.3% of novel

ORF Disrupted

121

12.6% of novel

Conserved

631

65.8% — same protein

912 of 959 novel isoforms (95.1%) have SwissProt protein homologs. 32 isoforms have no detectable protein homolog. Top species: Mus musculus (791), Homo sapiens (44), Bos taurus (37), Rattus norvegicus (28), Pongo abelii (2).

High-Impact Novel Isoforms (150)

Gene	Impact	Details	ORF	Reads	CPM	SwissProt
Chn1 `transcript1126.2.nic`	Domain altered	Lost: zf-RING_9	complete	141	310.2	sp\|P30337\|CHIN_RAT 99.6% — N-chimaerin
YWHAE `transcript1676.11.nic`	Domain altered	Lost: Ta0938	complete	88	193.6	sp\|P62261\|1433E_BOVIN 100.0% — 14-3-3 protein epsilon
Il18 `transcript835.9.nic`	Domain altered	Gained: GT-A	complete	50	110.0	sp\|P70380\|IL18_MOUSE 100.0% — Interleukin-18
RBBP4 `transcript1404.4.nic`	Domain altered	Lost: Hexon	complete	40	88.0	sp\|Q3MHL3\|RBBP4_BOVIN 95.5% — Histone-binding protein RBBP4
Grb14 `transcript1032.2.nic`	Domain altered	Lost: BPS, PH, Ubiquitin	complete	32	70.4	sp\|Q9JLM9\|GRB14_MOUSE 88.3% — Growth factor receptor-bound p...
Bin1 `transcript228.18.nnic`	Domain altered	Lost: CK2S, DUF1664, Jnk-SapK_ap_N, OmpH, PTS_IIA, SNARE-fusion, TBD	complete	32	70.4	sp\|O08539\|BIN1_MOUSE 93.9% — Myc box-dependent-interacting ...
MYL12B `transcript1998.17.nnic`	Domain altered	Lost: DUF2267, DUF2666, DUF3741, GPHH, NADP_Rossmann, Trigger_C, UPF0154	complete	23	50.6	sp\|O14950\|ML12B_HUMAN 63.4% — Myosin regulatory light chain ...
Cdc123 `transcript31.2.nnic`	Domain altered	Lost: Beta_propeller	complete	22	48.4	sp\|Q8CII2\|CD123_MOUSE 87.5% — Translation initiation factor ...
Igfbp4 `transcript2452.11.nic`	Domain altered	Lost: GF_recep_C-rich	complete	21	46.2	sp\|P47879\|IBP4_MOUSE 100.0% — Insulin-like growth factor-bin...
Gap43 `transcript896.16.nnic`	Domain altered	Lost: EF_hand, GMC, Neuromodulin_N	complete	21	46.2	—
Tpt1 `transcript1230.14.nnic`	Domain altered	Lost: DUF6613	complete	20	44.0	sp\|P63028\|TCTP_MOUSE 100.0% — Translationally-controlled tum...
Psmb10 `transcript1634.8.nic`	Domain altered	Gained: 6PGD_C	complete	20	44.0	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Cd81 `transcript3554.7.nnic`	Domain altered	Lost: DUF6768	complete	20	44.0	sp\|P35762\|CD81_MOUSE 100.0% — CD81 antigen
Laptm4a `transcript71.12.nnic`	Domain altered	Lost: Circovir2_Orf4	complete	20	44.0	sp\|Q60961\|LAP4A_MOUSE 100.0% — Lysosomal-associated transmemb...
Mmd2 `transcript2323.5.nnic`	Domain altered	Gained: Ferlin_C	complete	19	41.8	sp\|Q8R189\|PAQRA_MOUSE 100.0% — Monocyte to macrophage differe...
Thoc7 `transcript47.14.nnic`	Domain altered	Gained: Pec_lyase-like, Tup_N, V_ATPase_I	complete	19	41.8	sp\|Q7TMY4\|THOC7_MOUSE 100.0% — THO complex subunit 7 homolog
Nap1l1 `transcript1576.10.nnic`	Domain altered	Gained: SH3, TPR	complete	18	39.6	sp\|P28656\|NP1L1_MOUSE 99.4% — Nucleosome assembly protein 1-...
Nsg1 `transcript570.5.nic`	Domain altered	Gained: DUF1581, DUF2448, DUF535	complete	18	39.6	sp\|Q62092\|NSG1_MOUSE 100.0% — Neuronal vesicle trafficking-a...
Epn1 `transcript177.7.nnic`	Domain altered	Gained: DUF658	complete	17	37.4	sp\|Q80VP1\|EPN1_MOUSE 91.5% — Epsin-1
Khdrbs3 `transcript416.15.nnic`	Domain altered	Lost: FSA_C, STAR_dimer	complete	16	35.2	sp\|Q9R226\|KHDR3_MOUSE 100.0% — KH domain-containing, RNA-bind...
Nap1l1 `transcript1578.10.nnic`	Domain altered	Gained: Nucleoplasmin	complete	14	30.8	sp\|P28656\|NP1L1_MOUSE 99.0% — Nucleosome assembly protein 1-...
Kxd1 `transcript756.8.nnic`	Domain altered	Lost: Sde2_N_Ubi, TolA_bind_tri, Ubiquitin, Zn_Beta_Ribbon	complete	14	30.8	sp\|Q80XH1\|KXDL1_MOUSE 71.2% — KxDL motif-containing protein ...
Ubc `transcript1778.5.nnic`	Domain altered	Gained: DUF6393	complete	13	28.6	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Nsmce1 `transcript2794.7.nnic`	Domain altered	Gained: AbiJ_NTD3, TMEMspv1-c74-12, TraH	complete	13	28.6	sp\|Q9D720\|NSE1_MOUSE 95.1% — Non-structural maintenance of ...
Cops4 `transcript1027.5.nic`	Domain altered	Lost: YabA	complete	12	26.4	sp\|O88544\|CSN4_MOUSE 100.0% — COP9 signalosome complex subun...
Rbck1 `transcript2616.2.nnic`	Domain altered	Lost: Rad50_zn_hook, Ubiquitin, zf-RanBP	complete	12	26.4	sp\|Q9WUB0\|HOIL1_MOUSE 97.7% — RanBP-type and C3HC4-type zinc...
Ovol2 `transcript2436.2.nic`	Domain altered	Gained: Rubredoxin_2, YjdM_Zn_Ribbon	complete	11	24.2	sp\|Q8CIV7\|OVOL2_MOUSE 100.0% — Transcription factor Ovo-like ...
Mrps15 `transcript1260.4.nic`	Domain altered	Lost: GPS2_interact	complete	10	22.0	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
CPLX2 `transcript552.13.nnic`	Domain altered	Lost: DUF2838	complete	10	22.0	sp\|P84088\|CPLX2_BOVIN 100.0% — Complexin-2
Sharpin `transcript604.15.nic`	Domain altered	Lost: Emfourin, PH	complete	10	22.0	sp\|Q91WA6\|SHRPN_MOUSE 100.0% — Sharpin
Ckmt1 `transcript1845.2.nnic`	Domain altered	Lost: ATP-gua_PtransN	complete	9	19.8	sp\|P30275\|KCRU_MOUSE 100.0% — Creatine kinase U-type, mitoch...
Polb `transcript193.8.nnic`	Domain altered	Lost: HHH, WSN	complete	9	19.8	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Chmp3 `transcript661.6.nic`	Domain altered	Lost: SPOUT	complete	9	19.8	sp\|Q9CQ10\|CHMP3_MOUSE 100.0% — Charged multivesicular body pr...
Bax `transcript1583.7.nnic`	Domain altered	Lost: CF222	complete	8	17.6	sp\|Q07813\|BAX_MOUSE 100.0% — Apoptosis regulator BAX
Smap `transcript2609.7.nnic`	Domain altered	Lost: BUD22, HAD, NOA36, Nop14, Triple_barrel	complete	8	17.6	sp\|Q3MHL8\|SMAP_BOVIN 98.9% — Small acidic protein
Farsb `transcript831.1.nic`	Domain altered	Lost: Death	complete	8	17.6	sp\|Q9WUA2\|SYFB_MOUSE 100.0% — Phenylalanine--tRNA ligase bet...
Nap1l1 `transcript1571.10.nnic`	Domain altered	Gained: SH3, TPR	complete	7	15.4	sp\|P28656\|NP1L1_MOUSE 99.4% — Nucleosome assembly protein 1-...
Psmb10 `transcript1636.8.nic`	Domain altered	Gained: 6PGD_C	complete	7	15.4	sp\|O35955\|PSB10_MOUSE 100.0% — Proteasome subunit beta type-1...
Dctn6 `transcript326.8.nic`	Domain altered	Lost: Fucokinase	complete	7	15.4	sp\|Q9WUB4\|DCTN6_MOUSE 100.0% — Dynactin subunit 6
Gnptg `transcript465.17.nic`	Domain altered	Lost: AltA1	complete	7	15.4	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Iqcb1 `transcript821.16.nic`	Domain altered	Lost: MLD	complete	7	15.4	sp\|Q8BP00\|IQCB1_MOUSE 100.0% — IQ calmodulin-binding motif-co...
Fam107a `transcript97.14.nnic`	Domain altered	Lost: RNase_Y_N	complete	7	15.4	sp\|Q78TU8\|F107A_MOUSE 100.0% — Actin-associated protein FAM10...
Tmem175 `transcript1200.5.nic`	Domain altered	Lost: Yip1	complete	6	13.2	sp\|Q9CXY1\|TM175_MOUSE 100.0% — Endosomal/lysosomal proton cha...
Ndufs2 `transcript1769.1.nnic`	Domain altered	Lost: NiFeSe_Hases	complete	6	13.2	sp\|Q91WD5\|NDUS2_MOUSE 70.0% — NADH dehydrogenase [ubiquinone...
Zdhhc4 `transcript2388.5.nic`	Domain altered	Gained: DoxD-like	complete	6	13.2	sp\|Q9D6H5\|ZDHC4_MOUSE 100.0% — Palmitoyltransferase ZDHHC4
Kat5 `transcript359.19.nic`	Domain altered	Lost: SH3	complete	6	13.2	sp\|Q5RBG4\|KAT5_PONAB 100.0% — Histone acetyltransferase KAT5
Hey1 `transcript40.3.nic`	Domain altered	Lost: HTH	complete	6	13.2	sp\|Q9WV93\|HEY1_MOUSE 99.6% — Hairy/enhancer-of-split relate...
Fez1 `transcript433.9.nnic`	Domain altered	Gained: Sema4F_C	complete	6	13.2	sp\|P97577\|FEZ1_RAT 94.5% — Fasciculation and elongation p...
Ogdhl `transcript444.14.nnic`	Domain altered	Lost: THDP-binding, TKC_like	complete	6	13.2	sp\|D3ZQD3\|OGDHL_RAT 89.5% — 2-oxoglutarate dehydrogenase-l...
Rrp1 `transcript824.10.nic`	Domain altered	Lost: DUF4391, MMS19_N	complete	6	13.2	sp\|P56183\|RRP1_MOUSE 100.0% — Ribosomal RNA processing prote...
Pacrg `transcript84.17.nic`	Domain altered	Gained: Peptidase_PA, RNA_pol_Rpb2_2	complete	6	13.2	sp\|Q9DAK2\|PACRG_MOUSE 100.0% — Parkin coregulated gene protei...
Zc3h14 `transcript989.12.nic`	Domain altered	Lost: PWI-like	complete	6	13.2	sp\|Q8BJ05\|ZC3HE_MOUSE 77.4% — Zinc finger CCCH domain-contai...
Tipin `transcript1363.9.nnic`	Domain altered +NMD	Gained: His_Kinase_A	complete	5	11.0	sp\|Q91WA1\|TIPIN_MOUSE 96.4% — TIMELESS-interacting protein
Zcchc17 `transcript1530.4.nnic`	Domain altered	Lost: RR_TM4-6 Gained: DUF4604, Golgi_traff	complete	5	11.0	sp\|Q9ESX4\|ZCC17_MOUSE 100.0% — Zinc finger CCHC domain-contai...
Ubc `transcript1766.5.nnic`	Domain altered	Gained: DUF6393	complete	5	11.0	sp\|P0CG50\|UBC_MOUSE 100.0% — Polyubiquitin-C
Myl4 `transcript2862.11.nnic`	Domain altered	Gained: HTH	complete	5	11.0	sp\|P09541\|MYL4_MOUSE 100.0% — Myosin light chain 4
Commd1 `transcript319.11.nnic`	Domain altered	Lost: DUF4476	complete	5	11.0	sp\|Q8K4M5\|COMD1_MOUSE 100.0% — COMM domain-containing protein...
Prxl2a `transcript487.14.nic`	Domain altered	Lost: 2heme_cytochrom	complete	5	11.0	sp\|Q9CYH2\|PXL2A_MOUSE 100.0% — Peroxiredoxin-like 2A
Sh3glb2 `transcript597.2.nnic`	Domain altered	Lost: SH3, bZIP	complete	5	11.0	sp\|Q5PPJ9\|SHLB2_RAT 98.6% — Endophilin-B2
Ldb2 `transcript602.5.nnic`	Domain altered	Lost: LID, Vpu	complete	5	11.0	sp\|O55203\|LDB2_MOUSE 100.0% — LIM domain-binding protein 2
Coq2 `transcript1033.5.nic`	Domain altered	Lost: DUF2798	complete	4	8.8	sp\|Q66JT7\|COQ2_MOUSE 100.0% — 4-hydroxybenzoate polyprenyltr...
Clybl `transcript1458.14.nnic`	Domain altered	Lost: Omega_toxin	complete	4	8.8	sp\|Q8R4N0\|CLYBL_MOUSE 100.0% — Citramalyl-CoA lyase, mitochon...
Polb `transcript183.8.nnic`	Domain altered	Lost: HHH, WSN	complete	4	8.8	sp\|Q8K409\|DPOLB_MOUSE 100.0% — DNA polymerase beta
Bfar `transcript189.16.nic`	Domain altered	Gained: DUF2076	complete	4	8.8	sp\|Q8R079\|BFAR_MOUSE 99.7% — Bifunctional apoptosis regulat...
Blvra `transcript1961.2.nnic`	Domain altered	Lost: NADP_Rossmann, SAM	complete	4	8.8	sp\|Q9CY64\|BIEA_MOUSE 100.0% — Biliverdin reductase A
ACTL6B `transcript2126.5.nic`	Domain altered	Lost: Rep	complete	4	8.8	sp\|A4FUX8\|ACL6B_BOVIN 100.0% — Actin-like protein 6B
Snf8 `transcript2245.11.nic`	Domain altered	Lost: Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C, ZapD	complete	4	8.8	sp\|Q9CZ28\|SNF8_MOUSE 100.0% — Vacuolar-sorting protein SNF8
Becn1 `transcript2606.11.nic`	Domain altered	Lost: PP1c_bdg Gained: BCLiA, PI_PP_I	complete	4	8.8	sp\|O88597\|BECN1_MOUSE 100.0% — Beclin-1
Rpn2 `transcript2899.2.nic`	Domain altered	Lost: DUF5859, TPR, Ubiquitin	complete	4	8.8	sp\|Q9DBG6\|RPN2_MOUSE 100.0% — Dolichyl-diphosphooligosacchar...
Pagr1a `transcript3012.7.nnic`	Domain altered	Lost: Nop53, PAXIP1_C	complete	4	8.8	sp\|E9PUL5\|PRRT2_MOUSE 100.0% — Proline-rich transmembrane pro...
Bloc1s5 `transcript398.13.nnic`	Domain altered	Lost: Exonuc_VII_L, Stathmin, TPR	complete	4	8.8	sp\|Q8R015\|BL1S5_MOUSE 100.0% — Biogenesis of lysosome-related...
Tmem192 `transcript578.8.nic`	Domain altered +NMD	Gained: TMEM173	complete	4	8.8	sp\|Q9CXT7\|TM192_MOUSE 100.0% — Transmembrane protein 192
Tmem91 `transcript753.7.nnic`	Domain altered	Lost: AWPM-19	complete	4	8.8	sp\|Q8C581\|TMM91_MOUSE 100.0% — Transmembrane protein 91
Nol12 `transcript799.15.nic`	Domain altered	Lost: ALC	complete	4	8.8	sp\|Q8BG17\|NOL12_MOUSE 89.9% — Nucleolar protein 12
Cyp4f4 `transcript873.17.nnic`	Domain altered	Gained: PAS_Fold	complete	4	8.8	sp\|P51869\|CP4F4_RAT 91.0% — Cytochrome P450 4F4
Agbl4 `transcript919.4.nic`	Domain altered	Lost: ZnExoPePases	complete	4	8.8	sp\|Q09LZ8\|CBPC6_MOUSE 100.0% — Cytosolic carboxypeptidase 6
Nudt9 `transcript1067.5.nnic`	Domain altered	Gained: DUF6778	complete	3	6.6	sp\|Q8BVU5\|NUDT9_MOUSE 100.0% — ADP-ribose pyrophosphatase, mi...
Mrps15 `transcript1258.4.nnic`	Domain altered	Lost: DUF272, DUF4140, DUF724, GPS2_interact, MIX23, PIN	complete	3	6.6	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Mrps15 `transcript1261.4.nnic`	Domain altered	Lost: DUF272, DUF4140, DUF724, GPS2_interact, MIX23, PIN	complete	3	6.6	sp\|Q9DC71\|RT15_MOUSE 100.0% — Small ribosomal subunit protei...
Uchl5 `transcript1409.1.nic`	Domain altered +NMD	Gained: BBP1_C	complete	3	6.6	sp\|Q9WUP7\|UCHL5_MOUSE 100.0% — Ubiquitin carboxyl-terminal hy...
Msrb2 `transcript148.2.nnic`	Domain altered	Gained: Zn_Beta_Ribbon	complete	3	6.6	sp\|Q78J03\|MSRB2_MOUSE 100.0% — Methionine-R-sulfoxide reducta...
Kcnmb4 `transcript1603.10.nnic`	Domain altered +NMD	Lost: LapA_dom	complete	3	6.6	sp\|Q9JIN6\|KCMB4_MOUSE 100.0% — Calcium-activated potassium ch...
Nme7 `transcript1630.1.nnic`	Domain altered	Lost: PH	complete	3	6.6	sp\|Q9QXL8\|NDK7_MOUSE 100.0% — Nucleoside diphosphate kinase ...
Mrps31 `transcript173.8.nnic`	Domain altered	Gained: GREB1	complete	3	6.6	sp\|Q61733\|RT31_MOUSE 100.0% — Small ribosomal subunit protei...
Bcl7b `transcript1958.5.nnic`	Domain altered	Lost: BCL_N	complete	3	6.6	—
Gins4 `transcript211.8.nnic`	Domain altered	Lost: SLD5_C-like	complete	3	6.6	sp\|Q99LZ3\|SLD5_MOUSE 100.0% — DNA replication complex GINS p...
Aamdc `transcript2161.7.nnic`	Domain altered	Gained: HTH, PucR	complete	3	6.6	sp\|Q8R0P4\|AAMDC_MOUSE 97.5% — Mth938 domain-containing prote...
Bpgm `transcript239.6.nnic`	Domain altered	Lost: HTH	complete	3	6.6	sp\|P15327\|PMGE_MOUSE 100.0% — Bisphosphoglycerate mutase
Abhd12 `transcript2549.2.nic`	Domain altered	Lost: PDDEXK	complete	3	6.6	sp\|Q99LR1\|ABD12_MOUSE 100.0% — Lysophosphatidylserine lipase ...
Cntnap1 `transcript2582.11.nic`	Domain altered	Lost: EGF, PRIMA1	complete	3	6.6	sp\|O54991\|CNTP1_MOUSE 100.0% — Contactin-associated protein 1
Polg2 `transcript2960.11.nic`	Domain altered	Gained: EGF	complete	3	6.6	sp\|Q9QZM2\|DPOG2_MOUSE 100.0% — DNA polymerase subunit gamma-2
Sap30bp `transcript3164.11.nic`	Domain altered	Gained: CCDC84, Rib_recp_KP_reg	complete	3	6.6	sp\|Q9UHR5\|S30BP_HUMAN 98.0% — SAP30-binding protein
Slc35a4 `transcript380.18.nic`	Domain altered	Lost: DUF4535	complete	3	6.6	sp\|Q9D321\|S35A4_MOUSE 100.0% — Probable UDP-sugar transporter...
Tnnc1 `transcript396.14.nnic`	Domain altered	Lost: DUF6694, HRDC-like, Peptidase_CA, UPF0154	complete	3	6.6	sp\|P19123\|TNNC1_MOUSE 99.1% — Troponin C, slow skeletal and ...
Snx15 `transcript439.19.nic`	Domain altered	Lost: Gloverin	complete	3	6.6	sp\|Q91WE1\|SNX15_MOUSE 96.4% — Sorting nexin-15
Hepacam `transcript446.9.nnic`	Domain altered	Lost: Mog1p_PsbP	complete	3	6.6	sp\|Q640R3\|HECAM_MOUSE 100.0% — Hepatic and glial cell adhesio...
Gnptg `transcript467.17.nnic`	Domain altered	Lost: AltA1	complete	3	6.6	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Gnptg `transcript469.17.nnic`	Domain altered	Lost: AltA1	complete	3	6.6	sp\|Q6S5C2\|GNPTG_MOUSE 100.0% — N-acetylglucosamine-1-phosphot...
Pycr3 `transcript531.15.nnic`	Domain altered	Lost: Chorion_3, NADP_Rossmann	complete	3	6.6	sp\|Q9DCC4\|P5CR3_MOUSE 100.0% — Pyrroline-5-carboxylate reduct...
Rgs3 `transcript622.4.nic`	Domain altered	Lost: DUF4441	complete	3	6.6	sp\|Q9DC04\|RGS3_MOUSE 100.0% — Regulator of G-protein signali...
Sdr39u1 `transcript803.14.nic`	Domain altered	Lost: NADP_Rossmann	complete	3	6.6	sp\|Q5M8N4\|D39U1_MOUSE 100.0% — Epimerase family protein SDR39...
Iqcb1 `transcript822.16.nic`	Domain altered	Lost: MLD	complete	3	6.6	sp\|Q8BP00\|IQCB1_MOUSE 100.0% — IQ calmodulin-binding motif-co...
Cers2 `transcript937.3.nic`	Domain altered	Lost: HTH, TRAM1, UPF0767	complete	3	6.6	sp\|Q924Z4\|CERS2_MOUSE 100.0% — Ceramide synthase 2
Klc1 `transcript1467.12.nic`	Domain altered	Lost: COG2, TMF_TATA_bd, YabA Gained: DUF4919, MIT	complete	2	4.4	sp\|P37285\|KLC1_RAT 96.9% — Kinesin light chain 1
Abtb1 `transcript1059.6.nic`	Domain altered	Lost: Ank	complete	1	2.2	sp\|Q99LJ2\|ABTB1_MOUSE 99.7% — Ankyrin repeat and BTB/POZ dom...
Mbp `transcript1004.18.nnic`	ORF disrupted		5prime_partial	145	319.0	—
Ndrg2 `transcript591.14.nic`	ORF disrupted		5prime_partial	61	134.2	sp\|Q9QYG0\|NDRG2_MOUSE 96.2% — Protein NDRG2
Stx4 `transcript3097.7.nic`	ORF disrupted	Lost: COG2, Csm1_N, DUF3671, DUF3698, E-set, ETRAMP, His_Kinase_A, PRP1_N, T2SSF, TPR, TPR_MLP1_2, Vac_Fusion Gained: GOLD-like	3prime_partial	52	114.4	sp\|P70452\|STX4_MOUSE 100.0% — Syntaxin-4
Eno2 `transcript1542.6.nnic`	ORF disrupted	Lost: MBB, TIM_barrel	5prime_partial	49	107.8	sp\|P17183\|ENOG_MOUSE 94.4% — Gamma-enolase
Stoml2 `transcript423.4.nic`	ORF disrupted		5prime_partial	40	88.0	sp\|Q99JB2\|STML2_MOUSE 100.0% — Stomatin-like protein 2, mitoc...
Nptn `transcript1090.9.nic`	ORF disrupted		5prime_partial	35	77.0	sp\|P97546\|NPTN_RAT 98.7% — Neuroplastin
Cryzl1 `transcript1233.16.nic`	ORF disrupted		5prime_partial	27	59.4	sp\|Q921W4\|QORL1_MOUSE 100.0% — Quinone oxidoreductase-like pr...
Cibar1 `transcript69.4.nic`	ORF disrupted	Lost: Pec_lyase-like	5prime_partial	22	48.4	sp\|Q8BP22\|CBAR1_MOUSE 96.2% — CBY1-interacting BAR domain-co...
Nme1 `transcript2162.11.nic`	ORF disrupted	Gained: DUF6486	5prime_partial	19	41.8	sp\|P15532\|NDKA_MOUSE 85.1% — Nucleoside diphosphate kinase ...
Commd8 `transcript776.5.nnic`	ORF disrupted		5prime_partial	18	39.6	sp\|Q9CZG3\|COMD8_MOUSE 99.4% — COMM domain-containing protein...
Ntan1 `transcript213.16.nic`	ORF disrupted		5prime_partial	17	37.4	sp\|Q64311\|NTAN1_MOUSE 83.3% — Protein N-terminal asparagine ...
Prkar1a `transcript3004.11.nic`	ORF disrupted		5prime_partial	17	37.4	sp\|Q9DBC7\|KAP0_MOUSE 100.0% — cAMP-dependent protein kinase ...
Ifitm10 `transcript3499.7.nnic`	ORF disrupted		5prime_partial	16	35.2	—
Psmd8 `transcript957.7.nnic`	ORF disrupted		5prime_partial	16	35.2	sp\|Q9CX56\|PSMD8_MOUSE 80.2% — 26S proteasome non-ATPase regu...
Yaf2 `transcript1340.15.nic`	ORF disrupted	Lost: EGF, RING, WTX	5prime_partial	14	30.8	sp\|Q99LW6\|YAF2_MOUSE 100.0% — YY1-associated factor 2
Tpd52l2 `transcript3517.2.nic`	ORF disrupted	Lost: VBS-like	5prime_partial	13	28.6	sp\|Q9CYZ2\|TPD54_MOUSE 87.3% — Tumor protein D54
Uxs1 `transcript367.1.nic`	ORF disrupted	Gained: His_Kinase_A, OB, Pox_A_type_inc	5prime_partial	13	28.6	sp\|Q91XL3\|UXS1_MOUSE 100.0% — UDP-glucuronic acid decarboxyl...
Sucla2 `transcript1213.14.nnic`	ORF disrupted		5prime_partial	12	26.4	sp\|Q9Z2I9\|SUCB1_MOUSE 94.1% — Succinate--CoA ligase [ADP-for...
Ccdc106 `transcript153.7.nic`	ORF disrupted	Gained: E-set	5prime_partial	12	26.4	sp\|Q3ULM0\|CC106_MOUSE 100.0% — Coiled-coil domain-containing ...
Eef1b `transcript543.1.nnic`	ORF disrupted		5prime_partial	12	26.4	sp\|O70251\|EF1B_MOUSE 100.0% — Elongation factor 1-beta
Ntan1 `transcript209.16.nic`	ORF disrupted		5prime_partial	11	24.2	sp\|Q64311\|NTAN1_MOUSE 84.7% — Protein N-terminal asparagine ...
RPL19 `transcript2359.11.nnic`	ORF disrupted		5prime_partial	11	24.2	sp\|Q3T0W9\|RL19_BOVIN 97.9% — Large ribosomal subunit protei...
P33monox `transcript604.13.nic`	ORF disrupted		5prime_partial	11	24.2	sp\|Q9DBN4\|P33MX_MOUSE 100.0% — Putative monooxygenase p33MONO...
TXNL4A `transcript950.18.nnic`	ORF disrupted		3prime_partial	11	24.2	—
Pepd `transcript1260.7.nic`	ORF disrupted	Lost: AMP_N-like Gained: DUF3793	5prime_partial	10	22.0	sp\|Q11136\|PEPD_MOUSE 100.0% — Xaa-Pro dipeptidase
Mog `transcript1408.17.nnic`	ORF disrupted	Lost: PF	5prime_partial	10	22.0	sp\|Q61885\|MOG_MOUSE 97.1% — Myelin-oligodendrocyte glycopr...
YPEL3 `transcript2937.7.nic`	ORF disrupted		5prime_partial	10	22.0	sp\|A6QPH8\|YPEL3_BOVIN 100.0% — Protein yippee-like 3
Cadm1 `transcript789.9.nic`	ORF disrupted	Lost: DUF3439, Mid2, Mucin, SSP160, Tir_receptor_C	5prime_partial	10	22.0	sp\|Q8R5M8\|CADM1_MOUSE 90.5% — Cell adhesion molecule 1
Ephx4 `transcript1129.5.nic`	ORF disrupted		5prime_partial	9	19.8	sp\|Q6IE26\|EPHX4_MOUSE 99.0% — Epoxide hydrolase 4
Dhps `transcript1257.8.nic`	ORF disrupted		5prime_partial	9	19.8	sp\|Q3TXU5\|DHYS_MOUSE 100.0% — Deoxyhypusine synthase
Tsg101 `transcript1703.7.nic`	ORF disrupted	Gained: BLOC1_2, DUF3987, ERM_helical, Exonuc_VII_L	5prime_partial	9	19.8	sp\|Q61187\|TS101_MOUSE 100.0% — Tumor susceptibility gene 101 ...
Rtn4 `transcript379.11.nic`	ORF disrupted	Lost: DUF4736, Phage_holin_3_6	5prime_partial	9	19.8	sp\|Q99P72\|RTN4_MOUSE 100.0% — Reticulon-4
Urm1 `transcript546.2.nnic`	ORF disrupted		5prime_partial	9	19.8	sp\|Q9D2P4\|URM1_MOUSE 100.0% — Ubiquitin-related modifier 1
Yif1b `transcript967.7.nic`	ORF disrupted		5prime_partial	9	19.8	sp\|Q9CX30\|YIF1B_MOUSE 95.5% — Protein YIF1B
U2af1l4 `transcript1112.7.nic`	ORF disrupted		5prime_partial	8	17.6	sp\|Q8BGJ9\|U2AF4_MOUSE 96.3% — Splicing factor U2AF 26 kDa su...
Panx2 `transcript1235.15.nic`	ORF disrupted		5prime_partial	8	17.6	sp\|Q6IMP4\|PANX2_MOUSE 100.0% — Pannexin-2
Emg1 `transcript1460.6.nic`	ORF disrupted	Gained: MIF	5prime_partial	8	17.6	sp\|O35130\|NEP1_MOUSE 100.0% — Ribosomal RNA small subunit me...
Fam32a `transcript958.8.nic`	ORF disrupted		5prime_partial	8	17.6	sp\|Q9CR80\|FA32A_MOUSE 100.0% — Protein FAM32A
Cyp46a1 `transcript1233.12.nnic`	ORF disrupted		5prime_partial	7	15.4	sp\|Q9WVK8\|CP46A_MOUSE 100.0% — Cholesterol 24-hydroxylase
Hpcal1 `transcript138.12.nnic`	ORF disrupted		3prime_partial	7	15.4	sp\|P62748\|HPCL1_MOUSE 100.0% — Hippocalcin-like protein 1
Emc7 `transcript1609.2.nnic`	ORF disrupted		5prime_partial	7	15.4	sp\|Q9EP72\|EMC7_MOUSE 99.4% — Endoplasmic reticulum membrane...
Idh3B `transcript2055.2.nnic`	ORF disrupted	Lost: P-loop_NTPase	5prime_partial	7	15.4	sp\|Q68FX0\|IDH3B_RAT 89.1% — Isocitrate dehydrogenase [NAD]...
Fbxo44 `transcript2090.4.nic`	ORF disrupted		5prime_partial	7	15.4	sp\|Q8BK26\|FBX44_MOUSE 99.6% — F-box only protein 44
Ifitm10 `transcript3498.7.nic`	ORF disrupted	Lost: EF_hand, ScdA_N	5prime_partial	7	15.4	sp\|Q8BR26\|IFM10_MOUSE 100.0% — Interferon-induced transmembra...
Mcts1 `transcript417.X.nnic`	ORF disrupted	Lost: NSUN_ferredox	5prime_partial	7	15.4	sp\|Q4G009\|MCTS1_RAT 100.0% — Malignant T-cell-amplified seq...

Frame Impact Classification

Functional Diversity per Gene

Top Pfam Domains

Domain Architectures per Gene

Annotation QC: Pfam Artifact Detection

Related Pfam families are grouped into clans; proteins with anomalously high domain density (structural protein artifacts) are flagged separately. The cleaned set is used for all downstream analysis.

Original DV Genes

938

After QC Filtering

673

Removed (Artifacts)

265

Flagged Details

150

Flagged Artifacts (150)

Gene	Gene Name	Reason	Arch (raw)	Arch (collapsed)	Core / Variable Domains
`ENSMUSG00000032366`	TPM1	density artifact	7	0	39 / 28
`ENSMUSG00000027940`	Tpm3	density artifact	4	0	45 / 13
`ENSMUSG00000014402`	Tsg101	density artifact	4	0	43 / 5
`ENSMUSG00000055835`	Zfp1	density artifact	2	0	36 / 0
`ENSMUSG00000028464`	TPM2	density artifact	2	0	54 / 0
`ENSMUSG00000059689`	Znf32	density artifact	2	0	40 / 0
`ENSMUSG00000031851`	Ntpcr	density artifact	2	0	41 / 0
`ENSMUSG00000069208`	Znf431	density artifact	2	0	29 / 0
`ENSMUSG00000002985`	Apoe	density artifact	2	0	42 / 0
`ENSMUSG00000026761`	Orc4	density artifact	2	0	52 / 0
`ENSMUSG00000027273`	SNAP25	density artifact	2	0	28 / 0
`ENSMUSG00000026927`	Entr1	density artifact	2	0	28 / 0
`ENSMUSG00000007207`	Stx1a	density artifact	2	0	53 / 0
`ENSMUSG00000019370`	CALM1	density artifact	1	0	30 / 0
`ENSMUSG00000026966`	Ssna1	density artifact	1	0	55 / 0
`ENSMUSG00000042097`	Znf239	density artifact	1	0	31 / 0
`ENSMUSG00000020486`	Septin4	clan collapse	4	1	13 / 3
`ENSMUSG00000021548`	Ccnh	clan collapse	3	1	3 / 0
`ENSMUSG00000041438`	Utp4	clan collapse	3	1	7 / 1
`ENSMUSG00000051504`	CD33	clan collapse	4	2	7 / 0
`ENSMUSG00000011658`	Fuz	clan collapse	3	1	3 / 0
`ENSMUSG00000023051`	Tarbp2	clan collapse	3	1	3 / 1
`ENSMUSG00000026201`	Stk16	clan collapse	4	2	7 / 2
`ENSMUSG00000038671`	Arfrp1	clan collapse	3	1	7 / 2
`ENSMUSG00000034209`	Rasl10a	clan collapse	3	1	4 / 3
`ENSMUSG00000039183`	Nubp2	clan collapse	3	1	21 / 1
`ENSMUSG00000034744`	Nagk	clan collapse	2	1	1 / 1
`ENSMUSG00000020794`	UBE2G1	clan collapse	2	1	2 / 0
`ENSMUSG00000030701`	Plekhb1	clan collapse	2	1	2 / 1
`ENSMUSG00000033429`	Mcee	clan collapse	2	1	5 / 0
`ENSMUSG00000015806`	Qdpr	clan collapse	2	1	5 / 0
`ENSMUSG00000024217`	Snrpc	clan collapse	2	1	3 / 0
`ENSMUSG00000031505`	Naxd	clan collapse	2	1	3 / 0
`ENSMUSG00000032786`	Alas1	clan collapse	2	1	5 / 0
`ENSMUSG00000069835`	Sat2	clan collapse	2	1	8 / 0
`ENSMUSG00000026034`	Clk1	clan collapse	2	1	3 / 0
`ENSMUSG00000087260`	Lamtor5	clan collapse	2	1	2 / 0
`ENSMUSG00000013622`	Atraid	clan collapse	2	1	4 / 0
`ENSMUSG00000014554`	Dguok	clan collapse	2	1	5 / 0
`ENSMUSG00000025731`	Mettl26	clan collapse	2	1	6 / 1
`ENSMUSG00000049225`	Pdp1	clan collapse	2	1	2 / 0
`ENSMUSG00000028032`	Papss1	clan collapse	2	1	14 / 0
`ENSMUSG00000052456`	Get3	clan collapse	2	1	15 / 0
`ENSMUSG00000005150`	Wdr83	clan collapse	2	1	6 / 0
`ENSMUSG00000038374`	RBM8A	clan collapse	2	1	4 / 0
`ENSMUSG00000061232`	H2-K1	clan collapse	2	1	9 / 0
`ENSMUSG00000025499`	H-RAS	clan collapse	2	1	13 / 0
`ENSMUSG00000037894`	H2AZ1	clan collapse	2	1	3 / 0
`ENSMUSG00000055720`	Ubl7	clan collapse	2	1	6 / 0
`ENSMUSG00000042540`	Acot5	clan collapse	2	1	5 / 0
`ENSMUSG00000031641`	Cbr4	clan collapse	2	1	6 / 0
`ENSMUSG00000021982`	Cdadc1	clan collapse	2	1	4 / 0
`ENSMUSG00000024246`	Thumpd2	clan collapse	2	1	13 / 0
`ENSMUSG00000024190`	Dusp1	clan collapse	2	1	5 / 0
`ENSMUSG00000022110`	Sucla2	clan collapse	2	1	6 / 0
`ENSMUSG00000017715`	Pgs1	clan collapse	2	1	3 / 0
`ENSMUSG00000022721`	Trmt2a	clan collapse	2	1	21 / 0
`ENSMUSG00000020385`	Clk4	clan collapse	2	1	5 / 0
`ENSMUSG00000039637`	Coro7	clan collapse	2	1	10 / 0
`ENSMUSG00000068250`	Amn1	clan collapse	2	1	3 / 1
`ENSMUSG00000025086`	Trub1	clan collapse	2	1	2 / 0
`ENSMUSG00000073423`	Znf414	clan collapse	2	1	6 / 1
`ENSMUSG00000032475`	Nck1	clan collapse	2	1	8 / 0
`ENSMUSG00000030512`	SNRPA1	clan collapse	2	1	4 / 0
`ENSMUSG00000042104`	UGGT2	clan collapse	2	1	2 / 0
`ENSMUSG00000017307`	Acot8	clan collapse	2	1	2 / 1
`ENSMUSG00000039826`	Trub2	clan collapse	2	1	8 / 0
`ENSMUSG00000040532`	Abhd11	clan collapse	2	1	10 / 4
`ENSMUSG00000020873`	Slc35b1	clan collapse	2	1	5 / 0
`ENSMUSG00000007458`	M6pr	clan collapse	2	1	3 / 0
`ENSMUSG00000030272`	Camk1	clan collapse	2	1	9 / 0
`ENSMUSG00000045211`	Nudt18	clan collapse	2	1	2 / 0
`ENSMUSG00000033931`	Rbm34	clan collapse	2	1	7 / 0
`ENSMUSG00000078765`	U2af1l4	clan collapse	2	1	7 / 0
`ENSMUSG00000016637`	Ift27	clan collapse	2	1	7 / 0
`ENSMUSG00000029534`	ST7	clan collapse	2	1	8 / 0
`ENSMUSG00000058240`	Cryzl1	clan collapse	2	1	3 / 0
`ENSMUSG00000042729`	Wdr74	clan collapse	2	1	3 / 1
`ENSMUSG00000022635`	Zcrb1	clan collapse	2	1	6 / 0
`ENSMUSG00000018593`	Sparc	clan collapse	2	1	7 / 1
`ENSMUSG00000006728`	Cdk4	clan collapse	2	1	8 / 0
`ENSMUSG00000022756`	Slc7a4	clan collapse	2	1	4 / 0
`ENSMUSG00000009555`	Cdk9	clan collapse	2	1	6 / 0
`ENSMUSG00000006782`	Cnp	clan collapse	2	1	24 / 0
`ENSMUSG00000038975`	Rabggtb	clan collapse	2	1	3 / 0
`ENSMUSG00000022454`	Nell2	clan collapse	2	1	14 / 0
`ENSMUSG00000057411`	Antkmt	clan collapse	2	1	5 / 0
`ENSMUSG00000072214`	Septin5	clan collapse	2	1	14 / 0
`ENSMUSG00000032336`	Nptn	clan collapse	2	1	8 / 2
`ENSMUSG00000038286`	Bphl	clan collapse	2	1	12 / 0
`ENSMUSG00000002524`	Puf60	clan collapse	2	1	5 / 0
`ENSMUSG00000090841`	Myl6	clan collapse	2	1	6 / 0
`ENSMUSG00000022453`	Naga	clan collapse	2	1	4 / 0
`ENSMUSG00000004032`	Gstm5	clan collapse	2	1	6 / 0
`ENSMUSG00000051007`	Gatd1	clan collapse	2	1	2 / 0
`ENSMUSG00000023175`	Bsg	clan collapse	2	1	7 / 0
`ENSMUSG00000027998`	Plrg1	clan collapse	2	1	9 / 0
`ENSMUSG00000020612`	Prkar1a	clan collapse	2	1	4 / 0
`ENSMUSG00000063065`	Mapk3	clan collapse	2	1	9 / 0
`ENSMUSG00000021772`	Nkiras1	clan collapse	2	1	9 / 0
`ENSMUSG00000029203`	UBE2K	clan collapse	2	1	6 / 0
`ENSMUSG00000022456`	Septin3	clan collapse	2	1	25 / 0
`ENSMUSG00000047766`	Lrrc49	clan collapse	2	1	2 / 2
`ENSMUSG00000021134`	Srsf5	clan collapse	2	1	6 / 0
`ENSMUSG00000051730`	Mettl5	clan collapse	2	1	23 / 0
`ENSMUSG00000063428`	Ddo	clan collapse	2	1	8 / 0
`ENSMUSG00000053801`	Grwd1	clan collapse	2	1	6 / 0
`ENSMUSG00000022210`	Dhrs4	clan collapse	2	1	7 / 0
`ENSMUSG00000031812`	Map1lc3b	clan collapse	2	1	3 / 0
`ENSMUSG00000038784`	Cnot4	clan collapse	2	1	14 / 0
`ENSMUSG00000036775`	Decr2	clan collapse	2	1	5 / 0
`ENSMUSG00000059974`	Ntm	clan collapse	2	1	12 / 0
`ENSMUSG00000024516`	Sec11c	clan collapse	2	1	2 / 0
`ENSMUSG00000027679`	Dnajc19	clan collapse	2	1	2 / 0
`ENSMUSG00000007944`	Ttc9b	clan collapse	2	1	15 / 0
`ENSMUSG00000029462`	VPS29	clan collapse	2	1	2 / 0
`ENSMUSG00000039450`	Dcxr	clan collapse	2	1	12 / 0
`ENSMUSG00000027523`	Gnas	clan collapse	2	1	9 / 0
`ENSMUSG00000028969`	CDK5	clan collapse	2	1	7 / 0
`ENSMUSG00000051234`	Rnf7	clan collapse	2	1	8 / 0
`ENSMUSG00000056367`	Actr3b	clan collapse	2	1	5 / 0
`ENSMUSG00000025939`	Ube2w	clan collapse	2	1	3 / 0
`ENSMUSG00000046434`	HNRNPA1	clan collapse	2	1	6 / 0
`ENSMUSG00000025742`	Prps2	clan collapse	2	1	4 / 0
`ENSMUSG00000019054`	Fis1	clan collapse	2	1	8 / 0
`ENSMUSG00000045136`	TUBB2B	clan collapse	2	1	3 / 0
`ENSMUSG00000063235`	Ptpmt1	clan collapse	2	1	5 / 0
`ENSMUSG00000022570`	Gfus	clan collapse	2	1	6 / 0
`ENSMUSG00000039382`	Wdr45	clan collapse	2	1	4 / 0
`ENSMUSG00000028898`	Trnau1ap	clan collapse	2	1	3 / 0
`ENSMUSG00000037062`	Sh3glb1	clan collapse	2	1	6 / 1
`ENSMUSG00000035649`	Zcchc7	clan collapse	2	1	2 / 0
`ENSMUSG00000021111`	Papola	clan collapse	2	1	4 / 0
`ENSMUSG00000021877`	Arf4	clan collapse	2	1	9 / 1
`ENSMUSG00000044628`	Rnf208	clan collapse	2	1	10 / 0
`ENSMUSG00000028607`	Cpt2	clan collapse	2	1	2 / 0
`ENSMUSG00000000253`	Gmpr	clan collapse	2	1	4 / 0
`ENSMUSG00000028252`	Ccnc	clan collapse	2	1	4 / 0
`ENSMUSG00000026856`	Dolpp1	clan collapse	2	1	3 / 0
`ENSMUSG00000022474`	Pmm1	clan collapse	2	1	3 / 0
`ENSMUSG00000024862`	KLC2	clan collapse	2	1	38 / 0
`ENSMUSG00000029538`	Srsf9	clan collapse	2	1	5 / 0
`ENSMUSG00000055707`	Klhl26	clan collapse	2	1	8 / 0
`ENSMUSG00000019795`	Pcmt1	clan collapse	2	1	10 / 2
`ENSMUSG00000000184`	Ccnd2	clan collapse	2	1	1 / 2
`ENSMUSG00000027498`	Cstf1	clan collapse	2	1	8 / 0
`ENSMUSG00000042831`	Alkbh6	clan collapse	2	1	1 / 1
`ENSMUSG00000101892`	—	clan collapse	2	1	10 / 0
`ENSMUSG00000024576`	CSNK1A1	clan collapse	2	1	7 / 0
`ENSMUSG00000032540`	Abhd5	clan collapse	2	1	5 / 0

Domain Variants by Evidence Tier

Variable Domain Landscape

Total Variable Domains

445

Domain Loss Events

472

Domain Gain Events

Loss : Gain Ratio

27.8:1

Domain loss dominates gain ~28:1, consistent with alternative splicing predominantly removing exons encoding specific domains rather than inserting novel domain-encoding exons.

All Variable Domains (445)

Domain	DV Genes	Gain	Loss	Genes
`FapA`	5	0	5	Azi2, Ccdc30, Dtnbp1, Olfm1, Thoc7
`DUF1664`	5	0	5	Bin1, Ccdc30, Ccpg1, Immt, Olfm1
`ABC_tran_CTD`	3	0	3	Ing4, Olfm1, SPTBN5
`NPV_P10`	3	0	3	Dtnbp1, Olfm1, Tmem191
`TPR_MLP1_2`	3	0	3	CREM, Fgfr1op2, Stx4
`YabA`	3	0	3	Ccdc90b, Hnrnpc, Klc1
`Csm1_N`	3	0	3	Ccdc30, Olfm1, Stx4
`ATPase`	2	0	2	Ak6, Aqr
`Syntaxin_2`	2	0	2	Olfm1, Stx5
`Prominin`	2	0	2	App, Stx5
`Jnk-SapK_ap_N`	2	0	2	Bin1, Stx5
`Sec20`	2	0	2	Fgfr1op2, Tmem191
`ubiquitin`	2	0	2	Kxd1, Tecr
`DivIC`	2	0	2	Azi2, Bloc1s2
`Uso1_p115_C`	2	0	2	Bloc1s2, Dtnbp1
`ZapB`	2	0	2	Fgfr1op2, Olfm1
`UPF0242`	2	0	2	Azi2, Fgfr1op2
`DUF4446`	2	0	2	Fgfr1op2, Olfm1
`Occludin_ELL`	2	0	2	Fgfr1op2, Olfm1
`TMPIT`	2	0	2	Hnrnpc, Olfm1
`PRKG1_interact`	2	0	2	Azi2, Ccdc90b
`FlaC_arch`	2	0	2	Azi2, Ccdc30
`TMF_TATA_bd`	2	1	1	Azi2, Klc1
`SKI`	2	0	2	Ak6, Pstk
`Ank_5`	2	0	2	Abtb1, Acbd6
`Ank_2`	2	0	2	Abtb1, Acbd6
`Ank_3`	2	0	2	Abtb1, Acbd6
`Ank`	2	0	2	Abtb1, Acbd6
`Ank_4`	2	0	2	Abtb1, Acbd6
`DUF2076`	2	0	2	Bcas1, Ccdc30
`PRY`	2	1	1	Trim9, Fam107a
`Presenilin`	2	1	1	Cd47, RNPS1
`PRR20`	2	0	2	Fam241b, Tmem11
`ResIII`	1	0	1	Ddx5
`TFIID-18kDa`	1	0	1	Ddx5
`PCAF_N`	1	0	1	Pea15
`T2SSM`	1	1	0	UBE2I
`Sema4F_C`	1	0	1	Fez1
`Thump_like`	1	0	1	Naa10
`EH_Signature`	1	0	1	Nfs1
`Pyridoxal_deC`	1	0	1	Nfs1
`Peptidase_C48`	1	0	1	Nfs1
`GvpO`	1	0	1	Nfs1
`Binary_toxB_2`	1	0	1	Tpd52l1
`bZIP_Maf`	1	1	0	Sh3glb2
`YhhN`	1	0	1	Tusc3
`DUF6350`	1	0	1	Tusc3
`Bax1-I`	1	0	1	Tusc3
`Yip1`	1	0	1	Tusc3
`Phage_sheath_1N`	1	0	1	Ubc
`TPR_17`	1	0	1	Anapc5
`DUF4088`	1	0	1	Aqr
`GT-D`	1	0	1	Aqr
`DUF4240`	1	0	1	Aqr
`HTH_20`	1	0	1	Myl4
`TT_ORF2a`	1	0	1	Rab36
`DUF4193`	1	0	1	Shisa4
`SelR`	1	0	1	Slirp
`QWRF`	1	0	1	Stx5
`DUF4795`	1	0	1	Stx5
`Nuf2_DHR10-like`	1	0	1	Stx5
`WASH_WAHD`	1	0	1	Stx5
`Cep3`	1	0	1	Stx5
`Baculo_F`	1	0	1	Stx5
`ATG14`	1	0	1	Tmem191
`B56`	1	0	1	Mkrn1
`Prok-RING_4`	1	0	1	Mkrn1
`MKRN1_C`	1	0	1	Mkrn1
`HHH_3`	1	0	1	Polb
`HHH`	1	0	1	Polb
`HHH_8`	1	0	1	Polb
`HHH_5`	1	0	1	Polb
`HHH_2`	1	0	1	Polb
`IMS_HHH`	1	0	1	Polb
`WSN`	1	0	1	Polb
`DUF3701`	1	0	1	Polb
`DNA_pol_lambd_f`	1	0	1	Polb
`Cdd1`	1	0	1	Polb
`DUF6493`	1	0	1	Mbd3
`CDP-OH_P_tran_2`	1	0	1	Mtch2
`ESCRT-II`	1	1	0	Ramp2
`ExbD`	1	0	1	Ramp2
`DUF1376`	1	0	1	Ing4
`DASH_Spc19`	1	0	1	Ing4
`HCMV_UL139`	1	0	1	Ing4
`DUF1192`	1	0	1	Ing4
`AP3D1`	1	0	1	Ing4
`DASH_Dad2`	1	0	1	Ing4
`DUF4200`	1	0	1	Ing4
`DUF4407`	1	0	1	Ing4
`IFP_35_N`	1	0	1	Ing4
`PHD_2`	1	0	1	Ing4
`NAD_binding_2`	1	0	1	Blvrb
`DXP_reductoisom`	1	0	1	Blvrb
`Mito_fiss_reg`	1	0	1	Blvrb
`DFP`	1	0	1	Blvrb
`adh_short`	1	0	1	Blvrb
`CENP-Q`	1	0	1	Bloc1s2
`ZapA`	1	0	1	Bloc1s2
`UPF0449`	1	0	1	Bloc1s2
`TolA_bind_tri`	1	0	1	Fgfr1op2
`Prefoldin_2`	1	0	1	Fgfr1op2
`bZIP_1`	1	0	1	Fgfr1op2
`FIN1`	1	0	1	Fgfr1op2
`Cauli_AT`	1	0	1	Fgfr1op2
`Phage_GP20`	1	0	1	Fgfr1op2
`LUC7`	1	0	1	Fgfr1op2
`MAT1`	1	0	1	Fgfr1op2
`CHD5`	1	0	1	Fgfr1op2
`WXG100`	1	0	1	Fgfr1op2
`betaPIX_CC`	1	0	1	Fgfr1op2
`Snapin_Pallidin`	1	0	1	Fgfr1op2
`DUF2570`	1	0	1	Fgfr1op2
`EAD7`	1	0	1	Farsb
`DNA_pol_phi`	1	0	1	Apbb1
`PPP4R2`	1	0	1	Apbb1
`CHMI`	1	0	1	Emg1
`Nha1_C`	1	0	1	Phf23
`EBV-NA3`	1	0	1	Phf23
`Ribosomal_60s`	1	0	1	Phf23
`PHD_4`	1	0	1	Phf23
`Muted`	1	0	1	Dtnbp1
`Sec34`	1	0	1	Dtnbp1
`TRPM_tetra`	1	0	1	Dtnbp1
`Peptidase_S46`	1	0	1	Dtnbp1
`POTRA`	1	0	1	Dtnbp1
`TM1506`	1	1	0	UBA52
`Pilin_N`	1	0	1	Tmem276
`DUF6232`	1	0	1	Tmem276
`Nup35_RRM_2`	1	0	1	HNRNPA2B1
`NOA36`	1	0	1	Smap
`G6B`	1	0	1	Ccdc106
`LCD1`	1	1	0	Thyn1
`DUF2057`	1	0	1	Lin7b
`RecC_C`	1	0	1	Lin7b
`Jag_N`	1	0	1	Iscu
`Sec3-PIP2_bind`	1	0	1	Osbpl9
`BIR`	1	0	1	Rnf181
`Peptidase_S9`	1	0	1	Abhd12
`Abhydrolase_2`	1	0	1	Abhd12
`RAP`	1	0	1	Abhd12
`BD-FAE`	1	0	1	Abhd12
`DUF900`	1	0	1	Abhd12
`TRP_2`	1	0	1	Abhd12
`LIP`	1	0	1	Abhd12
`DUF6592`	1	0	1	Cuedc2
`PWI`	1	0	1	Zc3h14
`zf-CCCH_3`	1	0	1	Zc3h14
`E7`	1	0	1	NCALD
`SCNM1_acidic`	1	0	1	Scnm1
`Pox_F16`	1	0	1	Mettl23
`PrmA`	1	0	1	Mettl23
`IRK`	1	0	1	Glo1
`Cytadhesin_P30`	1	0	1	Hnrnpc
`Nup88`	1	0	1	Hnrnpc
`FUT8_N_cat`	1	0	1	Hnrnpc
`DUF3489`	1	0	1	Rpl13a
`DUF6777`	1	0	1	Nherf2
`DUF1319`	1	0	1	Azi2
`Cep57_MT_bd`	1	0	1	Azi2
`DUF1967`	1	0	1	Azi2
`Herpes_BLRF2`	1	0	1	Azi2
`DUF4629`	1	0	1	Fxr1
`eIF-3_zeta`	1	0	1	Fxr1
`Lycopene_cycl`	1	0	1	Txnrd2
`NAD_binding_7`	1	0	1	Txnrd2
`STAS`	1	1	0	Hdhd2
`FlbT`	1	1	0	Hdhd2
`FleQ`	1	1	0	Hdhd2
`Ribophorin_I`	1	0	1	Rpusd1
`DEAD_assoc`	1	0	1	Pstk
`GPS2_interact`	1	0	1	Mrps15
`VOMI`	1	0	1	Sars1
`PG_binding_2`	1	0	1	Sars1
`DUF2207`	1	0	1	Tmem134
`DUF3040`	1	0	1	Tmem134
`DUF308`	1	0	1	Tmem134
`SdpI`	1	0	1	Tmem134
`Neurensin`	1	0	1	Tmem134
`XRN1_DBM`	1	0	1	Tmub2
`DUF2813`	1	0	1	Pak1
`AAA_33`	1	0	1	Ak6
`NACHT`	1	0	1	Ak6
`PduV-EutP`	1	0	1	Ak6
`ADK`	1	0	1	Ak6
`Cytidylate_kin`	1	0	1	Ak6
`TsaE`	1	0	1	Ak6
`dNK`	1	0	1	Ak6
`Thymidylate_kin`	1	0	1	Ak6
`RuvB_N`	1	0	1	Ak6
`AAA_5`	1	0	1	Ak6
`AAA_30`	1	0	1	Ak6
`IstB_IS21`	1	0	1	Ak6
`AAA`	1	0	1	Ak6
`Mg_chelatase`	1	0	1	Ak6
`AAA_25`	1	0	1	Ak6
`AAA_22`	1	0	1	Ak6
`KTI12`	1	0	1	Ak6
`Cytidylate_kin2`	1	0	1	Ak6
`AAA_16`	1	0	1	Ak6
`AAA_14`	1	0	1	Ak6
`NTPase_1`	1	0	1	Ak6
`AAA_18`	1	0	1	Ak6
`AAA_24`	1	0	1	Ak6
`AAA_3`	1	0	1	Ak6
`AAA_28`	1	0	1	Ak6
`RNA_helicase`	1	0	1	Ak6
`AAA_17`	1	0	1	Ak6
`hEGF`	1	0	1	Bcan
`Sushi`	1	0	1	Bcan
`Laminin_EGF`	1	0	1	Bcan
`EGF`	1	0	1	Bcan
`EGF_2`	1	0	1	Bcan
`EGF_3`	1	0	1	Bcan
`EGF_CA`	1	0	1	Bcan
`Vpu`	1	0	1	Marchf2
`FAM184`	1	0	1	CLTA
`DUF6161`	1	0	1	CLTA
`GREB1`	1	0	1	Mrps31
`DUF2203`	1	0	1	Olfm1
`HSBP1`	1	0	1	Olfm1
`IL2`	1	0	1	Olfm1
`PilJ`	1	0	1	Olfm1
`Laminin_II`	1	0	1	Olfm1
`ATG16`	1	0	1	Olfm1
`FtsZ_C`	1	0	1	Olfm1
`SlyX`	1	0	1	Olfm1
`Fib_alpha`	1	0	1	Olfm1
`Baculo_PEP_C`	1	0	1	Olfm1
`Myosin_tail_1`	1	0	1	Olfm1
`Enkurin`	1	0	1	Olfm1
`EzrA`	1	0	1	Olfm1
`PriC`	1	0	1	Olfm1
`Helo_like_N`	1	0	1	Olfm1
`Cytochrom_B562`	1	0	1	Olfm1
`DUF5917`	1	0	1	Olfm1
`H-kinase_dim`	1	0	1	Olfm1
`Exonuc_VII_L`	1	0	1	Olfm1
`Csm2_III-A`	1	0	1	Olfm1
`Nup54`	1	0	1	Olfm1
`OLF`	1	0	1	Olfm1
`Spectrin`	1	0	1	Olfm1
`TBCC_N`	1	0	1	Olfm1
`FUSC`	1	0	1	Olfm1
`ATG17_like`	1	0	1	Olfm1
`Peptidase_M99_m`	1	0	1	Micu1
`EMP24_GP25L`	1	0	1	Stx4
`HisKA_3`	1	0	1	Stx4
`T2SSF`	1	0	1	Stx4
`Vac_Fusion`	1	0	1	Stx4
`Psg1`	1	0	1	Stx4
`DUF6584`	1	0	1	Stx4
`ETRAMP`	1	0	1	Stx4
`COG2`	1	0	1	Stx4
`PRP1_N`	1	0	1	Stx4
`DUF3671`	1	0	1	Stx4
`SDA1`	1	0	1	App
`Dicty_REP`	1	0	1	App
`PI3K_1B_p101`	1	0	1	App
`MerR`	1	0	1	Aamdc
`PucR`	1	0	1	Aamdc
`DUF4748`	1	0	1	Pnkd
`Zn_ribbon_SprT`	1	0	1	Znf431
`BDHCT_assoc`	1	0	1	Znf431
`zf-C2H2_11`	1	0	1	Znf431
`zf-Paramyx-P`	1	0	1	Znf431
`zf-C2H2`	1	0	1	Znf431
`zf-C2H2_6`	1	0	1	Znf431
`zf_UBZ`	1	0	1	Znf431
`zinc_ribbon_15`	1	0	1	Znf431
`ZZ`	1	0	1	Znf431
`C1_1`	1	0	1	Znf431
`C1_4`	1	0	1	Znf431
`zf-C2H2_jaz`	1	0	1	Znf431
`zf-C2H2_4`	1	0	1	Znf431
`zf-H2C2_2`	1	0	1	Znf431
`zf-trcl`	1	0	1	Znf431
`KRAB`	1	0	1	Znf431
`XPA_N`	1	0	1	Znf431
`Zn-ribbon_8`	1	0	1	Znf431
`Ribosomal_S6`	1	0	1	Ppil2
`RGS12_usC`	1	0	1	Ciapin1
`TSP_1`	1	0	1	Rnaseh2a
`PMSI1`	1	0	1	Rnaseh2a
`Nse4-Nse3_bdg`	1	0	1	Skic8
`PAS_3`	1	0	1	Cyp4f4
`DUF4919`	1	0	1	Klc1
`MIT`	1	1	0	Klc1
`SPRY`	1	0	1	Trim9
`ARGLU`	1	0	1	Immt
`SBP_bac_11`	1	0	1	Immt
`Rx_N`	1	0	1	Immt
`FAT`	1	0	1	Immt
`PA`	1	0	1	Rnf167
`Cactin_mid`	1	0	1	Necab3
`PhageMetallopep`	1	1	0	Mindy3
`YbgT_YccB`	1	0	1	Atp6v0b
`DUF3742`	1	0	1	Atp6v0b
`DUF948`	1	0	1	Ccpg1
`LIM`	1	0	1	Pdlim7
`zf-ribbon_3`	1	0	1	Pdlim7
`TF_Zn_Ribbon`	1	0	1	Pdlim7
`DZR`	1	0	1	Pdlim7
`SARAF`	1	0	1	Stk16
`DUF5898`	1	0	1	Stk16
`DUF573`	1	0	1	Fam13c
`SOGA`	1	0	1	Fam13c
`BAR_2`	1	0	1	Arfip2
`DiS_P_DiS`	1	0	1	YPEL3
`Elf1`	1	0	1	YPEL3
`EF-hand_2`	1	0	1	Tesc
`GIT_CC`	1	0	1	Tesc
`CHAP`	1	0	1	Fbxw2
`Ge1_WD40`	1	0	1	Fbxw2
`DUF1003`	1	0	1	Nkain4
`MmgE_PrpD`	1	0	1	Gfra4
`DUF4726`	1	0	1	Hspbp1
`DUF2231`	1	0	1	Cacfd1
`DUF4191`	1	0	1	Cacfd1
`Rubredoxin_2`	1	0	1	Araf
`VPS38`	1	0	1	Becn1
`PI_PP_I`	1	0	1	Becn1
`PP1c_bdg`	1	0	1	Becn1
`NPL`	1	1	0	Nap1l1
`GEN1_C`	1	0	1	Nap1l1
`CBF`	1	0	1	Nap1l1
`FSA_C`	1	0	1	Khdrbs3
`DUF4884`	1	0	1	Yars2
`S4`	1	0	1	Yars2
`FA_desaturase`	1	0	1	Pigp
`LPP20`	1	0	1	Stau1
`CUPID`	1	0	1	ENSMUSG00000078570
`FTCD_C`	1	0	1	ENSMUSG00000078570
`YcbB`	1	0	1	Usp16
`SWIRM-assoc_2`	1	0	1	Usp16
`DUF5442`	1	0	1	Usp16
`GvpL_GvpF`	1	0	1	Usp16
`V_ATPase_I`	1	0	1	Thoc7
`Tup_N`	1	0	1	Thoc7
`Hpr_kinase_N`	1	0	1	Tyw5
`CCDC23`	1	1	0	Josd2
`FAD-SLDH`	1	0	1	Rab3b
`RhlB`	1	0	1	Raly
`Filament_head`	1	0	1	Raly
`GRP`	1	0	1	Raly
`Herpes_capsid`	1	0	1	Raly
`DUF1129`	1	0	1	Tmem175
`SRP54_N`	1	0	1	Pex19
`Tht1`	1	0	1	Pex19
`mTERF`	1	0	1	Pex19
`HHH_4`	1	0	1	Pex19
`DUF4199`	1	0	1	Pex19
`DUF4391`	1	0	1	Rrp1
`MMS19_N`	1	0	1	Rrp1
`RIO1`	1	0	1	CAMK2A
`C1_2`	1	0	1	Yaf2
`Ephrin_rec_like`	1	0	1	Yaf2
`WTX`	1	0	1	Yaf2
`LMBR1`	1	1	0	Psma3
`Proteasome_A_N`	1	0	1	Psma3
`DUF6778`	1	0	1	Nudt9
`Tfb5`	1	0	1	Ndufab1
`pKID`	1	0	1	CREM
`TMCO5`	1	0	1	CREM
`IMCp`	1	0	1	CREM
`Mo25`	1	0	1	Chmp2a
`SPOUT_MTase`	1	0	1	Chmp3
`zf-ZPR1`	1	0	1	Chmp3
`Mcm10`	1	0	1	Chmp3
`FYDLN_acid`	1	0	1	Dync1i2
`DUF6465`	1	1	0	Dync1i2
`Anthrone_oxy`	1	0	1	Plpp5
`CK2S`	1	0	1	Bin1
`OmpH`	1	0	1	Bin1
`Arfaptin`	1	0	1	Bin1
`BAR_3`	1	0	1	Bin1
`TBD`	1	0	1	Bin1
`V-SNARE`	1	0	1	Bin1
`VP1_VP3`	1	0	1	Serbp1
`Tudor-knot`	1	0	1	Kat5
`Plant_tran`	1	0	1	Ccdc30
`CorA`	1	0	1	Ccdc30
`CCDC90-like`	1	0	1	Ccdc30
`Fungal_TACC`	1	0	1	Ccdc30
`Sipho_Gp157`	1	0	1	Ccdc30
`CENP-F_leu_zip`	1	0	1	Ccdc30
`T7SS_ESX_EspC`	1	0	1	Ccdc30
`DUF6262`	1	0	1	Ccdc30
`HALZ`	1	0	1	Ccdc30
`DUF5098`	1	0	1	Ccdc30
`FlxA`	1	0	1	Ccdc30
`LEDGF`	1	0	1	Psip1
`Rad50_zn_hook`	1	0	1	Rbck1
`zf-RING_2`	1	0	1	Rbck1
`DUF5959`	1	0	1	Rbck1
`zf-C3HC4`	1	0	1	Rbck1
`zf-RING_11`	1	0	1	Rbck1
`zf-RING_5`	1	0	1	Rbck1
`zf-RING_UBOX`	1	0	1	Rbck1
`zf-C3HC4_4`	1	0	1	Rbck1
`zf-C3HC4_2`	1	0	1	Rbck1
`Rad60-SLD`	1	0	1	Kxd1
`Ubiquitin_4`	1	0	1	Kxd1
`DUF2604`	1	0	1	Kxd1
`Ribosomal_L40e`	1	0	1	Kxd1
`TBK1_ULD`	1	0	1	Kxd1
`Ubiquitin_2`	1	0	1	Kxd1
`Sde2_N_Ubi`	1	0	1	Kxd1
`Rad60-SLD_2`	1	0	1	Kxd1
`Ubiquitin_5`	1	0	1	Kxd1
`HATPase_c_2`	1	0	1	Tipin
`DUF724`	1	0	1	Ccdc90b
`MHC_I_2`	1	0	1	Ccdc90b
`Activator-TraM`	1	0	1	Ccdc90b
`Lipoprotein_7`	1	0	1	Ccdc90b
`Herpes_DNAp_acc`	1	0	1	Eef1d
`Cytochrom_C_2`	1	0	1	Eef1d
`GlcNAc-1_reg`	1	0	1	Tcp1
`MLD`	1	0	1	Iqcb1
`Nucleo_P87`	1	0	1	Zfand3
`DAG_kinase_N`	1	0	1	Zfand3
`FYVE`	1	0	1	Zfand3
`zf-RanBP`	1	0	1	Ttc33
`CCD97-like_C`	1	0	1	Ttc33
`DUF2112`	1	0	1	Ttc33
`MASE3`	1	0	1	Hm13
`DNA_primase_lrg`	1	0	1	Rpl18a
`UPF0181`	1	0	1	Sf1
`Mid2`	1	0	1	Cadm1
`Tir_receptor_C`	1	0	1	Cadm1
`Mucin`	1	0	1	Cadm1
`RR_TM4-6`	1	0	1	Zcchc17
`DUF3827`	1	0	1	Zcchc17
`DUF2755`	1	0	1	Gpm6b
`GBP_C`	1	0	1	Stmn4
`CCDC84`	1	0	1	Stmn4
`AAA_23`	1	0	1	Stmn4
`Redoxin`	1	0	1	Rpl34
`TMEM173`	1	0	1	Tmem192
`ZapD`	1	0	1	Snf8
`IQ`	1	0	1	Gap43
`Neuromodulin_N`	1	0	1	Gap43
`Neuromodulin`	1	0	1	Gap43
`tRNA_anti-like`	1	0	1	Nars1
`tRNA_anti_2`	1	0	1	Nars1

Domain Variant Genes (673 genes, multiple architectures after QC)

All domain variant genes retaining multiple domain architectures after clan collapse and density artifact removal. Use the search box to filter by gene ID, name, function, or domain.

Showing 673 of 673 genes

Gene	Name	Function	Isoforms	Arch	Tier	CPM	Source	Core Domains
`ENSMUSG00000021288`	Klc1	Kinesin light chain 1	5	5	1	301.4	Mixed	ANAPC3, COG2, DUF627, GHL5, GlfT2_domain3, JIP_LZII, PPR, PPR_2, RNPP_C, RPN7, SHNi-TPR, SOGA, TPR_1, TPR_10, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_4, TPR_7, TPR_8, TPR_MalT
`ENSMUSG00000055692`	Tmem191	Transmembrane protein 191	4	4	1	37.4	Novel	ATG16, CheZ, Cnn_1N, DUF1664, DUF4407, FUSC, GAS, KASH_CCD, OmpH, RNase_Y_N, Spc24, Spc7, TMEM191C, pRN1_helical
`ENSMUSG00000035642`	Aamdc	Mth938 domain-containing protein	4	4	1	90.2	Mixed	DUF498, Indigoidine_A, UPF0180
`ENSMUSG00000028772`	Zcchc17	Zinc finger CCHC domain-containing protein 17	4	4	1	13.2	Novel	CDC45, Coilin_N, CpXC, DUF4604, Hid1, Menin, S1, SR-25, zf-CCHC
`ENSMUSG00000027618`	Nfs1	Cysteine desulfurase	3	3	1	46.2	Mixed	Aminotran_5, Beta_elim_lyase, DegT_DnrJ_EryC1
`ENSMUSG00000026860`	Sh3glb2	Endophilin-B2	6	3	1	206.8	Mixed	BAR, BAR_2, BAR_3, IMD, SH3_1, SH3_2, SH3_9
`ENSMUSG00000118664`	Tusc3	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TUSC3	3	3	1	50.6	Mixed	DUF4131, Gram_pos_anchor, OST3_OST6, Thioredoxin
`ENSMUSG00000008348`	Ubc	Polyubiquitin-C	5	3	1	129.8	Novel	Big_7, CFAP298, CorA, Crinkler, DUF1942, DUF2604, DUF3861, DUF5450, DUF6393, Dsc3_N, FlgD_ig, HemeBinding_Shp, LBP_C, ORF11CD3, Plexin_RBD, ProRS-C_2, Pullulanase_N2, Rad60-SLD, Rad60-SLD_2, Staphylokinase, TBK1_ULD, TmoB, UDP-g_GGTase, Ubiquitin_2, Ubiquitin_4, Ubiquitin_5, ubiquitin
`ENSMUSG00000040383`	Aqr	RNA helicase aquarius	3	3	1	19.8	Ref	AAA, AAA_11, AAA_12, AAA_16, AAA_19, AAA_22, AAA_30, Aquarius_N, DEAD, DUF2075, DnaB_C, Parvo_NS1, PhoH, RecA, ResIII, UvrD-helicase, UvrD_C_2
`ENSMUSG00000021040`	Slirp	SRA stem-loop-interacting RNA-binding protein, mitochondrial	3	3	1	22.0	Ref	Nup35_RRM, Nup35_RRM_2, PB1, RRM_1
`ENSMUSG00000029922`	Mkrn1	E3 ubiquitin-protein ligase makorin-1	4	3	1	22.0	Ref	Torus, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_4, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf-CCCH_4, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1, zf_CCCH_4
`ENSMUSG00000001240`	Ramp2	Receptor activity-modifying protein 2	5	3	1	129.8	Ref	RAMP
`ENSMUSG00000030330`	Ing4	Inhibitor of growth protein 4	3	3	1	112.2	Ref	DUF6482, ING, LMBR1, PHD, SOGA, zf-HC5HC2H
`ENSMUSG00000057506`	Bloc1s2	Biogenesis of lysosome-related organelles complex 1 subunit 2	4	3	1	202.4	Mixed	BLOC1_2, DUF948, Fib_alpha, HEF_HK, NPV_P10, ParB, Sec1, XhlA
`ENSMUSG00000040242`	Fgfr1op2	FGFR1 oncogene partner 2 homolog	3	3	1	48.4	Ref	Anthrax_toxA, DASH_Dam1, DUF16, Exonuc_VII_L, FUSC, FapA, Glyoxalase_8, Mgr1, NYD-SP28_assoc, PCRF, SIKE, TSC22, Tho2
`ENSMUSG00000018572`	Phf23	PHD finger protein 23	3	3	1	30.8	Ref	C1_1, PHD, PHD_2, Pox_Ag35, RNA_pol_3_Rpc31, zf-HC5HC2H, zf-RING_9
`ENSMUSG00000057531`	Dtnbp1	Dysbindin	3	3	1	52.8	Mixed	DUF2730, Dysbindin, FUSC
`ENSMUSG00000030663`	Smap	Small acidic protein	3	3	1	363.0	Mixed	BUD22, CDC45, Nop14, RNA_polI_A34, SMAP
`ENSMUSG00000004267`	Eno2	Gamma-enolase	4	3	1	358.6	Mixed	Enolase_C, Enolase_N, IAT_beta, MAAL_C, MR_MLE_C
`ENSMUSG00000003872`	Lin7b	Protein lin-7 homolog B	4	3	1	235.4	Novel	L27, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000045251`	ZNF688	Zinc finger protein 688	4	3	1	24.2	Mixed	FYVE_2, KRAB, OrfB_Zn_ribbon, TFIIS_C, Vps36-NZF-N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-ISL3, zf-LYAR, zf-met, zf_UBZ, zinc_ribbon_9
`ENSMUSG00000055850`	Rnf181	E3 ubiquitin-protein ligase RNF181	3	3	1	180.4	Novel	FANCL_C, PHD, Prok-RING_1, Prok-RING_4, RINGv, Rad50_zn_hook, UBZ_FAAP20, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000032046`	Abhd12	Lysophosphatidylserine lipase ABHD12	3	3	1	156.2	Novel	Abhydrolase_1, Abhydrolase_11, Abhydrolase_3, Abhydrolase_6, Hydrolase_4, Peptidase_S15, Say1_Mug180, Thioesterase
`ENSMUSG00000024142`	Mlst8	Target of rapamycin complex subunit LST8	3	3	1	22.0	Ref	ANAPC4_WD40, BRICHOS, DUF3463, DUF5854, Ge1_WD40, Hira, NBCH_WD40, Nup160, WD40
`ENSMUSG00000024026`	Glo1	Lactoylglutathione lyase	3	3	1	118.8	Ref	Glyoxalase, Glyoxalase_4, Glyoxalase_6, PIG-X
`ENSMUSG00000074129`	Rpl13a	Large ribosomal subunit protein uL13	3	3	1	1,791.0	Ref	Ribosomal_L13, SLT_L
`ENSMUSG00000002504`	Nherf2	Na(+)/H(+) exchange regulatory cofactor NHE-RF2	4	3	1	8.8	Ref	DUF1450, DUF5994, DUF6288, EBP50_C, GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50
`ENSMUSG00000039285`	Azi2	5-azacytidine-induced protein 2	3	3	1	19.8	Mixed	CENP-F_leu_zip, Cnn_1N, Csm1_N, DMPK_coil, DUF1090, DUF4407, HIP1_clath_bdg, Prefoldin_2, SurA_N, TBD, TPR_MLP1_2, YlqD, bZIP_2
`ENSMUSG00000027680`	Fxr1	RNA-binding protein FXR1	4	3	1	13.2	Ref	Agenet, FXMRP1_C_core, FXR_C1, FXR_C3, KH_1, KH_2, KH_4, KH_9, Tudor_2, Tudor_FRX1
`ENSMUSG00000028861`	Mrps15	Small ribosomal subunit protein uS15m	4	3	1	169.4	Novel	DUF272, DUF4140, DUF724, MIX23, PIN_4, Ribosomal_S15
`ENSMUSG00000068739`	Sars1	Serine--tRNA ligase, cytoplasmic	3	3	1	59.4	Ref	NOD, Seryl_tRNA_N, tRNA-synt_2b
`ENSMUSG00000028478`	CLTA	Clathrin light chain A	4	3	1	558.8	Ref	CENP-Q, Clathrin_lg_ch, ORC3_N
`ENSMUSG00000026833`	Olfm1	Noelin	3	3	1	851.5	Ref	BLOC1_2, CENP-Q, CLZ, DUF3450, Ephrin, MitMem_reg, Noelin-1, XhlA
`ENSMUSG00000030805`	Stx4	Syntaxin-4	3	3	1	28.6	Novel	AAA_13, ATG17_like, BLOC1_2, Baculo_PEP_C, COG5, DUF16, DUF1664, DUF3698, DUF5798, Desmo_N, ERp29, GlutR_dimer, Lectin_N, LuxT_C, NPV_P10, PilO, SNARE, Syntaxin, Syntaxin_2
`ENSMUSG00000022892`	App	Amyloid-beta precursor protein	3	3	1	1,007.7	Ref	APP_Cu_bd, APP_E2, APP_N, APP_amyloid, Activator-TraM, Beta-APP, DUF5033, Exonuc_VII_L, Insulin_TMD, Kunitz_BPTI, PilJ, Spore_III_AB
`ENSMUSG00000033793`	Atp6v1h	V-type proton ATPase subunit H	4	3	1	356.4	Mixed	Arm, Arm_2, DUF2326, HEAT, Mlp, SPOB_ab, UCH, V-ATPase_H_C, V-ATPase_H_N, YlbD_coat
`ENSMUSG00000022884`	EIF4A2	Eukaryotic initiation factor 4A-II	4	3	1	816.3	Ref	AAA_19, AAA_22, AAA_30, CMS1, DEAD, Fanconi_A, Flavi_DEAD, Helicase_C, Helicase_RecD, ResIII, UvrD-helicase, tRNA-synt_1b
`ENSMUSG00000027489`	Necab3	N-terminal EF-hand calcium-binding protein 3	4	3	1	107.8	Novel	ABM, DnaI_N, EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, Jnk-SapK_ap_N
`ENSMUSG00000033379`	Atp6v0b	V-type proton ATPase 21 kDa proteolipid subunit c''	3	3	1	1,432.3	Mixed	ATP-synt_C, G0-G1_switch_2, PIRT
`ENSMUSG00000021493`	Pdlim7	PDZ and LIM domain protein 7	4	3	1	189.2	Mixed	DUF4749, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Rv0078B
`ENSMUSG00000043259`	Fam13c	Protein FAM13C	3	3	1	4.4	Ref	Drc1-Sld2, SlyX, Tmemb_cc2, betaPIX_CC
`ENSMUSG00000015126`	Tsr3	18S rRNA aminocarboxypropyltransferase	6	3	1	81.4	Ref	RLI, Ribo_biogen_C
`ENSMUSG00000035086`	Becn1	Beclin-1	4	3	1	28.6	Mixed	ALMT, APG6, APG6_N, BH3, DASH_Hsk3, DUF3450, DUF4164, DUF4200, Exonuc_VII_L, FapA, GAS, HAUS-augmin3, MT, NAPRTase_C, Suppressor_APC, V_ATPase_I, YabA
`ENSMUSG00000058799`	Nap1l1	Nucleosome assembly protein 1-like 1	5	3	1	352.0	Novel	NAP
`ENSMUSG00000022332`	Khdrbs3	KH domain-containing, RNA-binding, signal transduction-associated protein 3	3	3	1	103.4	Mixed	KH_1, Qua1, STAR_dimer, Sam68-YY
`ENSMUSG00000022792`	Yars2	Tyrosine--tRNA ligase, mitochondrial	3	3	1	28.6	Ref	tRNA-synt_1b
`ENSMUSG00000025616`	Usp16	Ubiquitin carboxyl-terminal hydrolase 16	3	3	1	15.4	Ref	BRCT_assoc, DUF4638, DapB_C, RbpA, UCH, zf-UBP, zinc_ribbon_10, zinc_ribbon_9
`ENSMUSG00000053453`	Thoc7	THO complex subunit 7 homolog	3	3	1	140.8	Novel	AATF-Che1, COG2, Com_YlbF, DASH_Dad2, DUF1664, DUF4407, EcoEI_R_C, Med9, THOC7, UPF0193, ubiquitin
`ENSMUSG00000061032`	Rrp1	Ribosomal RNA processing protein 1 homolog A	3	3	1	404.8	Mixed	DNA_pol_phi, DRIM, DUF1981, DUF4776, Nop52, PFam54_60, Tn7_Tnp_TnsA_C
`ENSMUSG00000063889`	CREM	cAMP-responsive element modulator	3	3	1	19.8	Ref	KfrA_N, bZIP_1, bZIP_2, bZIP_Maf
`ENSMUSG00000053119`	Chmp3	Charged multivesicular body protein 3	3	3	1	52.8	Novel	DUF3915, PSD4, Snf7
`ENSMUSG00000027012`	Dync1i2	Cytoplasmic dynein 1 intermediate chain 2	5	3	1	17.6	Ref	ANAPC4_WD40, Dynein_IC2, SOBP, WD40
`ENSMUSG00000024381`	Bin1	Myc box-dependent-interacting protein 1	3	3	1	68.2	Mixed	BAR, BLOC1_2, Baculo_PEP_C, DUF5667, DUF948, Laminin_II, PTS_IIA, SH3_1, SH3_2, SH3_9, THP2, YtxH
`ENSMUSG00000021750`	Fam107a	Actin-associated protein FAM107A	7	3	1	8.8	Novel	DUF1151, KIAA1328, RNase_Y_N
`ENSMUSG00000028637`	Ccdc30	Coiled-coil domain-containing protein 30	3	3	1	6.6	Ref	DUF4686, RcsC
`ENSMUSG00000027466`	Rbck1	RanBP-type and C3HC4-type zinc finger-containing protein 1	3	3	1	26.4	Novel	IBR, ubiquitin, zf-RanBP
`ENSMUSG00000055553`	Kxd1	KxDL motif-containing protein 1	4	3	1	110.0	Mixed	KxDL, TolA_bind_tri
`ENSMUSG00000030613`	Ccdc90b	Coiled-coil domain-containing protein 90B, mitochondrial	3	3	1	22.0	Mixed	CCDC90-like, DUF3509, Vac_Fusion
`ENSMUSG00000044477`	Zfand3	AN1-type zinc finger protein 3	3	3	1	22.0	Ref	zf-A20, zf-AN1
`ENSMUSG00000032076`	Cadm1	Cell adhesion molecule 1	4	3	1	11.0	Mixed	Adeno_E3_CR1, Adhes-Ig_like, C1-set, C2-set, C2-set_2, DUF3439, I-set, Ig_2, Ig_3, Ig_4, Ig_C17orf99, Izumo-Ig, SSP160, Syndecan, V-set, ig
`ENSMUSG00000022044`	Stmn4	Stathmin-4	4	3	1	616.1	Ref	ATG14, DHR-2_Lobe_C, EF-hand_14, Exonuc_VII_L, FUT8_N_cat, Pex19, Ribonuc_2-5A, Stathmin, UPF0242
`ENSMUSG00000025521`	Tmem192	Transmembrane protein 192	3	3	1	33.0	Mixed	Flu_M2, O-ag_pol_Wzy, TMEM192
`ENSMUSG00000006058`	Snf8	Vacuolar-sorting protein SNF8	3	3	1	132.0	Mixed	DUF4896, EAP30, EutK_C, Phage_Mu_Gam, Plasmid_RAQPRD, RPA_interact_C
`ENSMUSG00000000296`	Tpd52l1	Tumor protein D53	3	3	4	11.0	Novel	Cnn_1N, CorA, DUF1818, DUF3458_C, DUF745, NUDE_C, TMEM247, TPD52, Uds1, WEMBL
`ENSMUSG00000015217`	Hmgb3	High mobility group protein B3	3	3	4	52.8	Mixed	CHDNT, CLEC16A_C, HMG_box, HMG_box_2, HMG_box_5, RNA_pol_3_Rpc31, Ribosomal_60s, YABBY
`ENSMUSG00000063179`	Pstk	L-seryl-tRNA(Sec) kinase	3	3	4	13.2	Novel	AAA_16, AAA_18, AAA_22, AAA_25, AAA_33, APS_kinase, DO-GTPase1, KTI12, LMBR1, MeaB, ParA, Zeta_toxin
`ENSMUSG00000003411`	Rab3b	Ras-related protein Rab-3B	4	3	4	55.0	Mixed	AAA_16, AAA_24, Arf, FeoB_N, Fin, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB
`ENSMUSG00000003464`	Pex19	Peroxisomal biogenesis factor 19	3	3	4	114.4	Ref	DUF3450, GlutR_dimer, Laminin_I, Pex19, Rod-binding
`ENSMUSG00000031570`	Plpp5	Phospholipid phosphatase 5	3	3	4	22.0	Ref	CoxIIa, PAP2
`ENSMUSG00000035521`	Gnptg	N-acetylglucosamine-1-phosphotransferase subunit gamma	6	2	1	114.4	Novel	AltA1, DPM3, PRKCSH, PRKCSH_1
`ENSMUSG00000020719`	Ddx5	Probable ATP-dependent RNA helicase DDX5	3	2	1	288.2	Novel	DEAD, ERCC3_RAD25_C, Helicase_C, Helicase_C_2, P68HR
`ENSMUSG00000013698`	Pea15	Astrocytic phosphoprotein PEA-15	3	2	1	107.8	Mixed	DED
`ENSMUSG00000030649`	Anapc15	Anaphase-promoting complex subunit 15	2	2	1	15.4	Ref	ANAPC15, Astro_capsid_p, BUD22, CDC45, DUF2457, Flavodoxin_4, Med8, Methyltransf_24, Methyltransf_3, Nop14, PCMT, Pox_Ag35, Presenilin, RNA_pol_Rpc4, TFB6, V_ATPase_I
`ENSMUSG00000015120`	UBE2I	SUMO-conjugating enzyme UBC9	6	2	1	83.6	Ref	Prok-E2_B, UQ_con
`ENSMUSG00000022200`	Golph3	Golgi phosphoprotein 3	2	2	1	26.4	Ref	GPP34, Peptidase_M15_2
`ENSMUSG00000032118`	Fez1	Fasciculation and elongation protein zeta-1	3	2	1	602.9	Novel	DUF5102, EURL, FEZ
`ENSMUSG00000025508`	Rplp2	Large ribosomal subunit protein P2	3	2	1	63.8	Ref	EF-hand_14, NolX, RelA_AH_RIS, Ribosomal_60s, TFIID_30kDa
`ENSMUSG00000008734`	Gprc5b	G-protein coupled receptor family C group 5 member B	2	2	1	24.2	Novel	7tm_3, DUF1467
`ENSMUSG00000073125`	Xlr3b	X-linked lymphocyte-regulated protein 3B	2	2	1	6.6	Ref	Anticodon_2, CHD5, CPG4, Cep57_MT_bd, Cor1, DUF1374, DUF1931, DUF349, Exonuc_VII_L, HEPN_RiboL-PSP, RHH_1, YlqD
`ENSMUSG00000018442`	Derl2	Derlin-2	2	2	1	11.0	Ref	DER1, Oleosin
`ENSMUSG00000002625`	Akap8l	A-kinase anchor protein 8-like	2	2	1	30.8	Ref	AKAP95, CDC45, SURF2, zf-C2H2_2
`ENSMUSG00000051355`	Commd1	COMM domain-containing protein 1	2	2	1	28.6	Novel	COMMD1_N, COMM_domain, DUF4476, SAM_KSR1
`ENSMUSG00000017493`	Igfbp4	Insulin-like growth factor-binding protein 4	2	2	1	8.8	Novel	IGFBP, Thyroglobulin_1
`ENSMUSG00000029126`	Nsg1	Neuronal vesicle trafficking-associated protein 1	2	2	1	127.6	Novel	Calcyon, DUF1581, DUF2448, DUF535
`ENSMUSG00000046240`	Hepacam	Hepatic and glial cell adhesion molecule	2	2	1	41.8	Novel	DUF3481, DcrB, I-set, Ig_2, Ig_3, Rhabdo_glycop, TMEM100, V-set, ig
`ENSMUSG00000018858`	Mrpl58	Large ribosomal subunit protein mL62	2	2	1	44.0	Ref	HLH, NOG1_N, RF-1, pEK499_p136
`ENSMUSG00000028309`	Rnf20	E3 ubiquitin-protein ligase BRE1A	2	2	1	4.4	Ref	CENP-F_leu_zip, CENP-K, CENP-Q, Csm1_N, DUF1664, DUF4763, DUF6468, Dynein_attach_N, HrpB7, POX, Phage_GPO, Prefoldin_2, Prok-RING_4, SpoIIE, TraF_2, ZapB, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000022253`	Nadk2	NAD kinase 2, mitochondrial	2	2	1	0.0	Ref	DAGK_cat, NAD_kinase, NAD_kinase_C
`ENSMUSG00000026171`	Rnf25	E3 ubiquitin-protein ligase RNF25	2	2	1	39.6	Ref	CpXC, DUF3785, Papo_T_antigen, RWD, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING_11, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000026199`	Ankzf1	tRNA endonuclease ANKZF1	2	2	1	4.4	Ref	ANAPC_CDC26, Ank, Ank_2, Ank_3, Ank_4, Ank_5, Cellulose_synt, DUF2514, DUF3464, Eapp_C, FAM176, FYDLN_acid, HscB_4_cys, Plasmodium_Vir, Presenilin, RNA_pol_3_Rpc31, RPA43_OB, RR_TM4-6, Rrn6, Sec62, SprA-related, TERB2, VATC, bVLRF1, zf-C2H2_jaz
`ENSMUSG00000035958`	Tdp2	Tyrosyl-DNA phosphodiesterase 2	2	2	1	11.0	Ref	Exo_endo_phos, UBA_4
`ENSMUSG00000022757`	TFG	Protein TFG	2	2	1	112.2	Novel	FAM181, MucBP, Ndc1_Nup, PB1
`ENSMUSG00000025290`	RPS24	Small ribosomal subunit protein eS24	4	2	1	1,108.9	Ref	Ribosomal_S24e, YrbL-PhoP_reg
`ENSMUSG00000029472`	Anapc5	Anaphase-promoting complex subunit 5	3	2	1	61.6	Ref	ANAPC5, MapZ_EC1, TPR_1, TPR_10, TPR_12, TPR_14, TPR_19, TPR_2, TPR_4, TPR_7, VP4_haemagglut, YycI
`ENSMUSG00000002289`	Angptl4	Angiopoietin-related protein 4	2	2	1	11.0	Ref	COG5, DUF16, DUF5798, DivIC, Fib_alpha, Fibrinogen_C, Helo_like_N, HrpB7, HrpJ, SlyX, UPF0242, XhlA
`ENSMUSG00000031171`	Ftsj1	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase	2	2	1	33.0	Ref	FtsJ, Methyltr_RsmB-F, Ubie_methyltran, Zn_Tnp_IS1595
`ENSMUSG00000027378`	Nphp1	Nephrocystin-1	2	2	1	13.2	Ref	DUF6100, Plasmodium_Vir, SH3_1, SH3_2, SH3_3, SH3_9
`ENSMUSG00000020372`	GNB2L1	Small ribosomal subunit protein RACK1	2	2	1	125.4	Ref	ANAPC4_WD40, Cytochrom_D1, DUF1416, Ge1_WD40, IKI3, NBCH_WD40, Nup160, PD40, WD40, WD40_like, eIF2A
`ENSMUSG00000061086`	Myl4	Myosin light chain 4	4	2	1	145.2	Novel	EF-hand_1, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9
`ENSMUSG00000019868`	Vta1	Vacuolar protein sorting-associated protein VTA1 homolog	2	2	1	48.4	Ref	DAG_kinase_N, Vta1, Vta1_C
`ENSMUSG00000020175`	Rab36	Ras-related protein Rab-36	3	2	1	11.0	Ref	AAA_14, Arf, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB
`ENSMUSG00000025545`	Clybl	Citramalyl-CoA lyase, mitochondrial	3	2	1	63.8	Novel	ACI44, C-C_Bond_Lyase, HpcH_HpaI
`ENSMUSG00000024072`	Yipf4	Protein YIPF4	2	2	1	26.4	Ref	ASTER, Yip1
`ENSMUSG00000041112`	Elmo1	Engulfment and cell motility protein 1	2	2	1	147.4	Ref	ELMO_ARM, ELMO_CED12, FERM_N, Imm42, Nup96, PH_12, ZFYVE21_C
`ENSMUSG00000010110`	Stx5	Syntaxin-5	3	2	1	37.4	Ref	Apolipoprotein, BLOC1_2, DUF16, DUF1664, DUF5063, DUF5082, Fib_alpha, IZUMO, LPP, MCPsignal, NPV_P10, SKA1, SNARE, Spectrin, Syntaxin, Syntaxin-5_N, Troponin, Vac_Fusion
`ENSMUSG00000016481`	Cr1l	Complement component receptor 1-like protein	3	2	1	26.4	Mixed	ASFV_J13L, Adeno_E3_CR2, Cyt_c_ox_IV, DUF3290, EphA2_TM, MP_p6, PspB, RIFIN, Sushi, Vpu, cEGF
`ENSMUSG00000097487`	Ptges3l	Putative protein PTGES3L	2	2	1	17.6	Ref	ANAPC15, CS, Nod_GRP, SR-25
`ENSMUSG00000024787`	Snx15	Sorting nexin-15	2	2	1	79.2	Novel	Gloverin, MIT, PX
`ENSMUSG00000032040`	Dcps	m7GpppX diphosphatase	2	2	1	44.0	Novel	DcpS, DcpS_C
`ENSMUSG00000021546`	HNRNPK	Heterogeneous nuclear ribonucleoprotein K	3	2	1	114.4	Ref	DJ-1_PfpI, DUF719, KH_1, KH_2, KH_4, KH_5, MOEP19, ROKNT
`ENSMUSG00000024175`	Tekt4	Tektin-4	2	2	1	17.6	Ref	APG6_N, ATG16, BORCS8, BST2, Baculo_PEP_C, CLZ, DUF1664, DUF2730, DivIC, EBP50_C, Mating_N, Med9, MitMem_reg, Noelin-1, PI3K_P85_iSH2, Prefoldin, Spc24, TMF_DNA_bd, Tektin, Uso1_p115_C, XhlA
`ENSMUSG00000030538`	Cib1	Calcium and integrin-binding protein 1	2	2	1	50.6	Ref	Dockerin_1, EF-hand_1, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, NuiA, SPARC_Ca_bdg, S_100
`ENSMUSG00000007570`	FANCE	Fanconi anemia group E protein	2	2	1	8.8	Ref	Bystin, FA_FANCE
`ENSMUSG00000003873`	Bax	Apoptosis regulator BAX	3	2	1	92.4	Novel	BID, Bcl-2, Bclx_interact, CF222
`ENSMUSG00000025420`	Katnal2	Katanin p60 ATPase-containing subunit A-like 2	2	2	1	11.0	Ref	AAA, AAA_14, AAA_16, AAA_18, AAA_22, AAA_24, AAA_25, AAA_28, AAA_33, AAA_5, AAA_7, AAA_lid_3, DUF815, IstB_IS21, LisH, Mg_chelatase, NACHT, NB-ARC, Parvo_NS1, PhoH, RNA_helicase, RuvB_N, TIP49, TsaE, bpMoxR
`ENSMUSG00000031536`	Polb	DNA polymerase beta	3	2	1	116.6	Novel	DNA_pol_B_palm, DNA_pol_B_thumb, NTP_transf_2
`ENSMUSG00000033287`	KCTD17	BTB/POZ domain-containing protein KCTD17	2	2	1	72.6	Novel	BTB, BTB_2, Peptidase_M90, SAM_LFY
`ENSMUSG00000000355`	Mcts1	Malignant T-cell-amplified sequence 1	3	2	1	101.2	Novel	DUF1947, Methyltr_RsmF_N, PUA, Pre-PUA
`ENSMUSG00000035478`	Mbd3	Methyl-CpG-binding domain protein 3	3	2	1	28.6	Ref	MBD, MBD_C, MBDa, NUMOD1, RasGAP
`ENSMUSG00000027282`	Mtch2	Mitochondrial carrier homolog 2	3	2	1	266.2	Ref	Mito_carr
`ENSMUSG00000030750`	Nsmce1	Non-structural maintenance of chromosomes element 1 homolog	2	2	1	116.6	Novel	AbiJ_NTD3, C1_1, DZR_2, SMC_Nse1, TMEMspv1-c74-12, TraH, zf-C3HC4, zf-C3HC4_2, zf-RING-like
`ENSMUSG00000053291`	Rab4b	Ras-related protein Rab-4B	2	2	1	323.4	Ref	AAA_16, AAA_22, AAA_7, ATPase, ATPase_2, Arf, DUF2075, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Ras, Roc, RsgA_GTPase, SRPRB, Septin, UvrD-helicase, cobW
`ENSMUSG00000011114`	Tbrg1	Transforming growth factor beta regulator 1	2	2	1	30.8	Ref	FYRC, FYRN
`ENSMUSG00000020076`	Ddx50	ATP-dependent RNA helicase DDX50	2	2	1	52.8	Novel	AAA_11, AAA_22, AAA_30, CMS1, DEAD, ERCC3_RAD25_C, GUCT, Helicase_C, Presenilin, ResIII
`ENSMUSG00000026279`	Thap4	Peroxynitrite isomerase THAP4	2	2	1	2.2	Ref	Csm1_N, DUF4201, HrpB7, Med21, THAP, THAP4_heme-bd, TMF_DNA_bd, Tropomyosin_1, YscO
`ENSMUSG00000027487`	Cdk5rap1	Mitochondrial tRNA methylthiotransferase CDK5RAP1	2	2	1	4.4	Ref	Radical_SAM, TRAM, UPF0004
`ENSMUSG00000006575`	Rundc3a	RUN domain-containing protein 3A	3	2	1	158.4	Ref	DUF3450, DUF5595, Phage_GP20, RUN, UPF0242, WT1
`ENSMUSG00000006288`	Ttc5	Tetratricopeptide repeat protein 5	2	2	1	74.8	Ref	ANAPC3, Abi_C, CK2S, DUF6584, MAS20, RPN7, TPR-S, TPR_1, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_8, TPR_9, TTC5_OB
`ENSMUSG00000015714`	Cers2	Ceramide synthase 2	3	2	1	30.8	Novel	Homeodomain, Homez, TRAM1, TRAM_LAG1_CLN8, UPF0767
`ENSMUSG00000063457`	RPS15	Small ribosomal subunit protein uS19	2	2	1	1,238.7	Ref	LCAT, MAP65_ASE1, Prok_Ub, Ribosomal_S19
`ENSMUSG00000076432`	Ywhaq	14-3-3 protein theta	2	2	1	180.4	Ref	14-3-3, AAA_15, FliM, XhlA
`ENSMUSG00000061613`	U2AF1	Splicing factor U2AF 35 kDa subunit	3	2	1	231.0	Ref	KMP11, Nup35_RRM_2, RRM_1, Torus, zf-CCCH, zf-CCCH_2, zf-CCCH_3, zf_CCCH_4
`ENSMUSG00000013155`	Enkd1	Enkurin domain-containing protein 1	2	2	1	15.4	Ref	Chibby, Enkurin
`ENSMUSG00000040263`	Klhdc4	Kelch domain-containing protein 4	2	2	1	26.4	Ref	DUF4110, Kelch_1, Kelch_2, Kelch_3, Kelch_4, Kelch_5, Kelch_6
`ENSMUSG00000002980`	Bcam	Basal cell adhesion molecule	2	2	1	6.6	Ref	C1-set, C2-set_2, Filaggrin, I-set, Ig_2, Ig_3, Ig_4, Ig_5, Ig_6, Izumo-Ig, V-set, ig
`ENSMUSG00000014856`	Tmem208	Transmembrane protein 208	4	2	1	114.4	Ref	DUF2207, DUF4191, Halogen_Hydrol, TMEM208_SND2
`ENSMUSG00000037601`	Nme1	Nucleoside diphosphate kinase A	2	2	1	103.4	Novel	DUF6486, NDK
`ENSMUSG00000031546`	Gins4	DNA replication complex GINS protein SLD5	2	2	1	39.6	Novel	CRR7, SLD5_C, Sld5
`ENSMUSG00000029860`	Zyx	Zyxin	2	2	1	15.4	Ref	LIM, Mut7-C, zf-dskA_traR, zf_C2H2_13
`ENSMUSG00000025142`	Aspscr1	Tether containing UBX domain for GLUT4	2	2	1	57.2	Ref	Cas9_C, QWRF, RBD, TUG-UBL1, UBX
`ENSMUSG00000024939`	Fam89b	Leucine repeat adapter protein 25	2	2	1	19.8	Ref	LURAP, MAGI_u5
`ENSMUSG00000021759`	Plpp1	Phospholipid phosphatase 1	2	2	1	41.8	Novel	DUF418, DUF6541, PAP2, PAP2_C
`ENSMUSG00000022571`	Pycr3	Pyrroline-5-carboxylate reductase 3	2	2	1	66.0	Novel	2-Hacid_dh_C, ApbA, Chorion_3, DapB_N, F420_oxidored, NAD_Gly3P_dh_N, NAD_binding_2, P5CR_dimer, PDH_N, Shikimate_DH
`ENSMUSG00000026701`	Prdx6	Peroxiredoxin-6	3	2	1	453.2	Ref	1-cysPrx_C, AhpC-TSA, Redoxin
`ENSMUSG00000031708`	Tecr	Very-long-chain enoyl-CoA reductase	3	2	1	2,063.8	Ref	Steroid_dh
`ENSMUSG00000001247`	Lsr	Lipolysis-stimulated lipoprotein receptor	2	2	1	6.6	Ref	Ig_3, LSR, V-set
`ENSMUSG00000027361`	Gabpb1	GA-binding protein subunit beta-1	2	2	1	4.4	Ref	AAA_23, Ank, Ank_2, Ank_3, Ank_4, Ank_5, CCDC84, CREPT, GrpE, LAMTOR5
`ENSMUSG00000040466`	Blvrb	Flavin reductase (NADPH)	3	2	1	22.0	Ref	3Beta_HSD, Epimerase, NAD_binding_10, NmrA, Semialdhyde_dh, TrkA_N
`ENSMUSG00000035203`	Epn1	Epsin-1	2	2	1	44.0	Novel	ANTH, DUF658, ENTH, UIM, VHS
`ENSMUSG00000063550`	Nup98	Nuclear pore complex protein Nup98-Nup96	2	2	1	15.4	Ref	ANAPC3, Nucleoporin2, Nucleoporin_FG, Nup96
`ENSMUSG00000014769`	Psmb1	Proteasome subunit beta type-1	2	2	1	63.8	Ref	Cytochrom_C1, FAM165, Proteasome
`ENSMUSG00000020849`	YWHAE	14-3-3 protein epsilon	2	2	1	204.6	Novel	14-3-3, Orbi_VP5, Ta0938
`ENSMUSG00000031431`	Tsc22d3	TSC22 domain family protein 3	2	2	1	224.4	Novel	DUF2452, DUF5660, DUF6262, DivIC, Fzo_mitofusin, K-box, NPV_P10, TBK1_CCD1, TSC22, YabA, ZapB
`ENSMUSG00000037740`	Mrps26	Small ribosomal subunit protein mS26	2	2	1	61.6	Ref	DUF1127, MAJIN, MRP-S26, PBP_sp32
`ENSMUSG00000039706`	Ldb2	LIM domain-binding protein 2	2	2	1	11.0	Novel	LID, LIM_bind, Vpu, zf-DNL
`ENSMUSG00000021486`	RAB24	Ras-related protein Rab-24	2	2	1	752.5	Ref	AAA_24, PRELI, Ras, Roc
`ENSMUSG00000044533`	Rps2	Small ribosomal subunit protein uS5	3	2	1	74.8	Novel	Ribosomal_S5, Ribosomal_S5_C
`ENSMUSG00000025967`	Eef1b	Elongation factor 1-beta	3	2	1	74.8	Mixed	EF-1_beta_acid, EF1_GNE, GST_C_3, GST_C_6
`ENSMUSG00000026245`	Farsb	Phenylalanine--tRNA ligase beta subunit	3	2	1	50.6	Mixed	B3_4, B5, PhetRS_B1, tRNA_synthFbeta
`ENSMUSG00000022684`	Bfar	Bifunctional apoptosis regulator	2	2	1	6.6	Novel	Baculo_IE-1, DUF2076, Prok-RING_4, SAM_1, SAM_2, SAM_PNT, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000004070`	Hmox2	Heme oxygenase 2	2	2	1	74.8	Ref	CENP-H, DUF349, Heme_oxygenase, SsgA
`ENSMUSG00000037032`	Apbb1	Amyloid beta precursor protein binding family B member 1	4	2	1	422.4	Ref	PID, PID_2, PTB, WW
`ENSMUSG00000066456`	Hmgn3	High mobility group nucleosome-binding domain-containing protein 3	2	2	1	226.6	Ref	HMG14_17, TT_ORF2, UPF0239
`ENSMUSG00000004268`	Emg1	Ribosomal RNA small subunit methyltransferase NEP1	3	2	1	151.8	Novel	EMG1
`ENSMUSG00000061315`	NACA	Nascent polypeptide-associated complex subunit alpha	2	2	1	1,152.9	Novel	DUF5529, HYPK_UBA, NAC, Podoplanin, RNA_pol_Rpo13
`ENSMUSG00000025646`	Atrip	ATR-interacting protein	2	2	1	11.0	Ref	FapA, Fez1, Golgin_A5, HIP1_clath_bdg, HMMR_N, Lebercilin, Med21, OmpH, RNase_H_2, RNase_T, Tropomyosin_1
`ENSMUSG00000033272`	Slc35a4	Probable UDP-sugar transporter protein SLC35A4	3	2	1	28.6	Novel	DUF4535, EamA, Nuc_sug_transp
`ENSMUSG00000002871`	Tpra1	Transmembrane protein adipocyte-associated 1	2	2	1	15.4	Novel	DUF3681, Peptidase_C21, Tmemb_40
`ENSMUSG00000090137`	UBA52	Ubiquitin-ribosomal protein eL40 fusion protein	5	2	1	167.2	Ref	DUF2604, Dsc3_N, Rad60-SLD, Rad60-SLD_2, Ribosomal_L40e, TBK1_ULD, Ubiquitin_2, Ubiquitin_4, Ubiquitin_5, ubiquitin
`ENSMUSG00000024914`	Drap1	Dr1-associated corepressor	2	2	1	184.8	Ref	CBFD_NFYB_HMF, Histone, PPP4R2, TAF4
`ENSMUSG00000022552`	Sharpin	Sharpin	2	2	1	74.8	Novel	Emfourin, Sharpin_PH, ubiquitin, zf-RanBP
`ENSMUSG00000026888`	Grb14	Growth factor receptor-bound protein 14	2	2	1	83.6	Novel	BPS, PH, PH_20, RA, SH2
`ENSMUSG00000016503`	Gtf3a	Transcription factor IIIA	2	2	1	90.2	Ref	C1_2, CCAP, CHORD, FOXP-CC, Ribosomal_S14, Spt46, zf-C2H2, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_8, zf-C2H2_aberr, zf-C2H2_jaz, zf-C2HC_2, zf-C2HE, zf-Di19, zf-H2C2_2, zf-H2C2_5, zf-met, zf_Hakai, zf_ZIC
`ENSMUSG00000015804`	Med28	Mediator of RNA polymerase II transcription subunit 28	2	2	1	63.8	Ref	DUF6530, Filament, HSCB_C, HTH_34, Med28, NPV_P10, PSD2, Sec2p
`ENSMUSG00000036503`	Rnf13	E3 ubiquitin-protein ligase RNF13	2	2	1	13.2	Ref	Baculo_11_kDa, CTU2, DNA_ligase_ZBD, FeoB_associated, HTH_63, LapA_dom, Neur_chan_memb, Ninjurin, PA, PHD, Prok-RING_4, RINGv, SID-1_RNA_chan, zf-ANAPC11, zf-C3H2C3, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000056116`	H2-Q10	H-2 class I histocompatibility antigen, Q10 alpha chain	2	2	1	11.0	Ref	AA_permease_2, C1-set, C2-set_2, Cadherin_C_2, Ig_3, MHC_I, Mucin, ig
`ENSMUSG00000064289`	Tank	TRAF family member-associated NF-kappa-B activator	2	2	1	8.8	Ref	DUF1090, DUF5082, Exonuc_VII_L, HAUS6_N, MAD, MYRF_ICA, OEP, PKcGMP_CC, PspA_IM30, TBD, UPF0242
`ENSMUSG00000038005`	Hpf1	Histone PARylation factor 1	3	2	1	136.4	Ref	DUF2228, DUF6027
`ENSMUSG00000057363`	Uxs1	UDP-glucuronic acid decarboxylase 1	2	2	1	24.2	Novel	3Beta_HSD, Epimerase, GDP_Man_Dehyd, HPTransfase, NAD_binding_4, Polysacc_synt_2, Pox_A_type_inc, Rep-A_N, RmlD_sub_bind, UXS1_N
`ENSMUSG00000121584`	Tmem276	Transmembrane protein 276	3	2	1	156.2	Ref	SdpI
`ENSMUSG00000076439`	Mog	Myelin-oligodendrocyte glycoprotein	2	2	1	182.6	Novel	Adeno_E4, I-set, Ig_2, Ig_3, Longin, V-set, ig
`ENSMUSG00000027270`	Lamp5	Lysosome-associated membrane glycoprotein 5	2	2	1	442.2	Ref	Chordopox_A13L, Cons_hypoth698, DUF4083, DUF819, Lamp, VAS1_LD
`ENSMUSG00000040888`	Dst	Dystonin	2	2	1	13.2	Ref	APOBEC4, DUF5344, DUF6730, Evr1_Alr, FAD_SOX, GrpE, NCKAP5, Nbl1_Borealin_N, SNAD4, Spectrin
`ENSMUSG00000028318`	Polr1e	DNA-directed RNA polymerase I subunit RPA49	2	2	1	2.2	Ref	RNA_pol_I_A49, SfsA_N
`ENSMUSG00000036040`	Adamtsl2	ADAMTS-like protein 2	2	2	1	4.4	Novel	ADAMTS_CR_3, ADAMTS_spacer1, Barttin, PLAC, TSP1_ADAMTS, TSP1_CCN, TSP1_spondin, TSP_1
`ENSMUSG00000004980`	HNRNPA2B1	Heterogeneous nuclear ribonucleoproteins A2/B1	3	2	1	24.2	Ref	HnRNPA1, OB_RNB, RRM_1, RRM_7
`ENSMUSG00000044811`	Cd300c2	CMRF-35-like molecule 4	2	2	1	30.8	Ref	Apq12, Ig_3, V-set, ig
`ENSMUSG00000030795`	Fus	RNA-binding protein FUS	2	2	1	22.0	Ref	KleE, RRM_1, zf-RanBP
`ENSMUSG00000021660`	Btf3	Transcription factor BTF3	2	2	1	688.7	Ref	LolA, Myco_19_kDa, NAC
`ENSMUSG00000040276`	Pacsin1	Protein kinase C and casein kinase substrate in neurons protein 1	5	2	1	35.2	Ref	DUF2605, FCH, RPS31, SH3_1, SH3_2, SH3_9, Terpene_syn_C_2
`ENSMUSG00000015094`	Npdc1	Neural proliferation differentiation and control protein 1	2	2	1	904.3	Ref	LapA_dom, NPDC1
`ENSMUSG00000050711`	Scg2	Secretogranin-2	2	2	1	44.0	Ref	Granin, IF2_N, eIF3_N
`ENSMUSG00000032172`	OLFM2	Noelin-2	2	2	1	158.4	Ref	ABC_tran_CTD, CLZ, DUF1664, Ephrin, Helo_like_N, Matrilin_ccoil, Noelin-1, OLF, PepSY_2
`ENSMUSG00000054863`	TAFA5	Chemokine-like protein TAFA-5	2	2	1	4.4	Ref	BSP_II, CDC45, CENP-B_dimeris, DUF4637, PRR20, Radial_spoke, TAFA, WRNPLPNID
`ENSMUSG00000021007`	Spata7	Spermatogenesis-associated protein 7 homolog	2	2	1	17.6	Ref	APG5, HSD3
`ENSMUSG00000023150`	Ivns1abp	Influenza virus NS1A-binding protein homolog	2	2	1	6.6	Ref	BACK, BTB, CTD8, IreB, Kelch_1, Kelch_2, Kelch_3, Kelch_4, Kelch_5, Kelch_6, PQQ_3, XPA_C
`ENSMUSG00000035228`	Ccdc106	Coiled-coil domain-containing protein 106	4	2	1	46.2	Novel	CCDC106, SOGA
`ENSMUSG00000035824`	Tk2	Thymidine kinase 2, mitochondrial	2	2	1	6.6	Ref	AAA_18, AAA_33, CoaE, RE_Bpu10I, Thymidylate_kin, dNK
`ENSMUSG00000037196`	Pacrg	Parkin coregulated gene protein homolog	2	2	1	11.0	Novel	DCB, ParcG, Peptidase_C107, RNA_pol_Rpb2_2
`ENSMUSG00000035443`	Thyn1	Thymocyte nuclear protein 1	5	2	1	110.0	Mixed	EVE
`ENSMUSG00000049625`	Tifab	TRAF-interacting protein with FHA domain-containing protein B	2	2	1	0.0	Ref	Ap4A_phos_N, FHA
`ENSMUSG00000051674`	Dcun1d4	DCN1-like protein 4	2	2	1	8.8	Ref	Cullin_binding, DUF6564
`ENSMUSG00000050043`	Tmx2	Thioredoxin-related transmembrane protein 2	2	2	1	220.0	Ref	PAP2_3, Thioredoxin
`ENSMUSG00000025825`	Iscu	Iron-sulfur cluster assembly enzyme ISCU	3	2	1	277.2	Ref	NifU_N
`ENSMUSG00000042354`	Gnl3	Guanine nucleotide-binding protein-like 3	2	2	1	26.4	Ref	BRD4_CDT, Dynamin_N, FeoB_N, GN3L_Grn1, GTP_EFTU, MMR_HSR1, MeaB, Nicastrin, RsgA_GTPase
`ENSMUSG00000039917`	Rhbdd2	Rhomboid domain-containing protein 2	2	2	1	11.0	Ref	DER1, DUF2070, Rhomboid
`ENSMUSG00000031371`	Haus7	HAUS augmin-like complex subunit 7	2	2	1	37.4	Ref	AATF-Che1, FlgN, Nsp1_C, Plant_NMP1, YabA
`ENSMUSG00000034403`	Pja1	E3 ubiquitin-protein ligase Praja-1	2	2	1	77.0	Mixed	Amidase, FANCL_C, zf-ANAPC11, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-Nse, zf-P11, zf-RING_11, zf-RING_16, zf-RING_2, zf-RING_5, zf-RING_UBOX, zf-rbx1
`ENSMUSG00000028411`	Aptx	Aprataxin	2	2	1	37.4	Ref	CBP_BcsO, DcpS_C, FHA, FHA_2, Glycoamylase, HIT, TAF8_C, zf-AN1, zf-C2H2_4, zf-C2H2_6, zf-C2HE
`ENSMUSG00000040414`	Slc25a28	Mitoferrin-2	2	2	1	4.4	Ref	Mito_carr, SAT, SLC25_like
`ENSMUSG00000025878`	Uimc1	BRCA1-A complex subunit RAP80	2	2	1	4.4	Ref	DUF1645, LIDHydrolase, RAP80_UIM, Rad50_zn_hook, UBZ_FAAP20, UIM, XAF1_C, zf_UBZ
`ENSMUSG00000045777`	Ifitm10	Interferon-induced transmembrane protein 10	2	2	1	79.2	Novel	CD225, Internalin_N, ScdA_N
`ENSMUSG00000051504`	CD33	Myeloid cell surface antigen CD33	4	2	1	28.6	Ref	C2-set, C2-set_2, DUF2490, I-set, Ig_3, V-set, ig
`ENSMUSG00000021913`	Ogdhl	2-oxoglutarate dehydrogenase-like, mitochondrial	2	2	1	59.4	Novel	2-oxogl_dehyd_N, E1_dh, OxoGdeHyase_C, Transket_pyr
`ENSMUSG00000022415`	Syngr1	Synaptogyrin-1	2	2	1	275.0	Ref	Col_cuticle_N, DUF4064, DUF805, Fig1, MARVEL, Ost5
`ENSMUSG00000028559`	Osbpl9	Oxysterol-binding protein-related protein 9	3	2	1	8.8	Ref	FapA, Oxysterol_BP, PH, PH_11, PH_20, PH_6, PH_8
`ENSMUSG00000026575`	Nme7	Nucleoside diphosphate kinase homolog 7	4	2	1	17.6	Novel	Arg_repressor, DM10_dom, NDK
`ENSMUSG00000056486`	Chn1	N-chimaerin	2	2	1	682.1	Novel	C1_1, FYVE_2, PHD, PHD_2, RhoGAP, zf-RING-like, zf-RING_9
`ENSMUSG00000074923`	Pak6	Serine/threonine-protein kinase PAK 6	2	2	1	4.4	Ref	ABC1, APH, DUF3915, DZR, DZR_2, FAM27, Kdo, Kinase-like, PBD, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000019194`	Scn1b	Sodium channel regulatory subunit beta-1	2	2	1	116.6	Novel	DUF6264, Ig_2, Ig_3, Protocadherin, V-set, ig
`ENSMUSG00000036748`	Cuedc2	CUE domain-containing protein 2	6	2	1	257.4	Mixed	CUE
`ENSMUSG00000056851`	Pcbp2	Poly(rC)-binding protein 2	4	2	1	96.8	Ref	Bac_chlorC, KH_1, KH_2, KH_4, KH_5, MOEP19
`ENSMUSG00000028137`	Celf3	CUGBP Elav-like family member 3	2	2	1	13.2	Ref	BRAP2, Nup35_RRM, Nup35_RRM_2, PHM7_cyt, Peripla_BP_4, RNA_bind, RRM_1, RRM_3, RRM_7, TFIIA
`ENSMUSG00000030032`	Wdr54	WD repeat-containing protein 54	2	2	1	41.8	Ref	ANAPC4_WD40, Anillin, Lgl_C, PQQ, RAB3GAP2_N, WD40
`ENSMUSG00000021012`	Zc3h14	Zinc finger CCCH domain-containing protein 14	3	2	1	17.6	Mixed	zf-CCCH_2
`ENSMUSG00000006476`	Nsmf	NMDA receptor synaptonuclear signaling and neuronal migration factor	2	2	1	28.6	Mixed	DUF4347, IQ
`ENSMUSG00000021792`	Prxl2a	Peroxiredoxin-like 2A	3	2	1	158.4	Novel	AhpC-TSA, AhpC-TSA_2, DUF2231, Redoxin, UCR_Fe-S_N
`ENSMUSG00000021248`	Tmed10	Transmembrane emp24 domain-containing protein 10	2	2	1	151.8	Ref	CrgA, EMP24_GP25L, TRPM_tetra
`ENSMUSG00000038982`	Bloc1s5	Biogenesis of lysosome-related organelles complex 1 subunit 5	2	2	1	2.2	Novel	APC_rep, DUF5557, Exonuc_VII_L, Muted, Stathmin
`ENSMUSG00000048796`	Cyb561d1	Probable transmembrane reductase CYB561D1	2	2	1	4.4	Ref	Cytochrom_B561, DUF5325, DUF6249
`ENSMUSG00000051359`	NCALD	Neurocalcin-delta	4	2	1	24.2	Novel	Dockerin_1, EF-hand_1, EF-hand_10, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, SPARC_Ca_bdg, WEF-hand
`ENSMUSG00000092607`	Scnm1	Sodium channel modifier 1	3	2	1	44.0	Mixed	zf-SCNM1
`ENSMUSG00000090266`	Mettl23	Histone-arginine methyltransferase METTL23	3	2	1	74.8	Ref	Methyltransf_16
`ENSMUSG00000031843`	Mphosph6	M-phase phosphoprotein 6	2	2	1	50.6	Ref	MPP6, TilS
`ENSMUSG00000020912`	Krt12	Keratin, type I cytoskeletal 12	2	2	1	61.6	Novel	ADIP, APC_N_CC, Bacillus_HBL, COG2, DUF1664, DUF4407, DivIC, ERM_helical, Filament, LPP, Mod_r, SKA1, Sec2p, Sec34, Tweety, Uso1_p115_C, V_ATPase_I, YabA
`ENSMUSG00000022559`	Fbxl6	F-box/LRR-repeat protein 6	2	2	1	19.8	Ref	F-box, F-box-like, LRR_4, LRR_6, LRR_8
`ENSMUSG00000038520`	Tbc1d17	TBC1 domain family member 17	2	2	1	52.8	Ref	DEC-1_N, PH_RBD, RabGAP-TBC, Y1_Tnp
`ENSMUSG00000099689`	ZNF383	Zinc finger protein 383	2	2	1	2.2	Ref	BHD_1, BolA, C1_4, DUF3741, DUF5830, DUF968, GAGA, KRAB, Raffinose_syn, TFIIS_C, XPA_N, Zn-ribbon_8, Zn_Tnp_IS1595, Zn_ribbon_SprT, zf-BED, zf-C2H2, zf-C2H2_11, zf-C2H2_3rep, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HE, zf-H2C2_2, zf-met, zf-trcl, zf_C2H2_13, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000023307`	MARCHF5	E3 ubiquitin-protein ligase MARCHF5	2	2	1	46.2	Ref	DUF2517, FANCL_C, PHD, Phage_holin_2_1, RINGv, zf-CSL, zf-RING_2, zf-RING_4
`ENSMUSG00000039660`	Spout1	28S rRNA (uridine-N(3))-methyltransferase	2	2	1	37.4	Ref	AP1AR, DUF2514, Methyltrn_RNA_3
`ENSMUSG00000060373`	Hnrnpc	Heterogeneous nuclear ribonucleoproteins C1/C2	5	2	1	114.4	Ref	RRM_1, SKA1, Saf4_Yju2, XhlA
`ENSMUSG00000039533`	Mmd2	Monocyte to macrophage differentiation factor 2	2	2	1	22.0	Novel	DUF2842, Ferlin_C, HlyIII
`ENSMUSG00000075467`	Dnlz	DNL-type zinc finger protein	2	2	1	4.4	Ref	DUF6112, DUF6510, HypA, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-CSL, zf-DNL
`ENSMUSG00000017999`	Ddx27	Probable ATP-dependent RNA helicase DDX27	2	2	1	4.4	Ref	AAA_22, CDC45, DEAD, Helicase_C, PRK, Pns11_12, Presenilin, RR_TM4-6, ResIII, UTP25, Vfa1
`ENSMUSG00000060038`	Dhps	Deoxyhypusine synthase	4	2	1	68.2	Mixed	Carbam_trans_N, DS
`ENSMUSG00000034826`	Nup54	Nuclear pore complex protein Nup54	2	2	1	8.8	Novel	FAST_2, Nup54, Nup54_C, TBCC_N, XhlA
`ENSMUSG00000022856`	Tmem41a	Transmembrane protein 41A	2	2	1	30.8	Ref	SNARE_assoc, ThiW
`ENSMUSG00000028576`	Ift74	Intraflagellar transport protein 74 homolog	2	2	1	26.4	Ref	AAA_13, APG6_N, ATG17_like, BLOC1_2, DUF1192, DUF4140, DUF4763, DUF5917, DUF6376, DivIC, FPP, Fez1, GAS, GIT_CC, HALZ, LPP, Leu_zip, NPV_P10, PMSR, SOGA, Seryl_tRNA_N, Spc7, Spectrin, TMF_DNA_bd
`ENSMUSG00000030556`	Lrrc28	Leucine-rich repeat-containing protein 28	2	2	1	2.2	Ref	LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, SPAN-X
`ENSMUSG00000029610`	Aimp2	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2	2	2	1	116.6	Ref	AIMP2_LysRS_bd, GST_C, GST_C_2, GST_C_3, GST_C_6, GTP-bdg_M, Thioredoxin_16
`ENSMUSG00000013593`	Ndufs2	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial	2	2	1	305.8	Novel	Complex1_49kDa, NiFeSe_Hases
`ENSMUSG00000001018`	Snapin	SNARE-associated protein Snapin	2	2	1	77.0	Ref	CATRA-C, COG2, CorA, DUF1664, DUF5798, E2F_TDP, NPV_P10, Snapin_Pallidin
`ENSMUSG00000023094`	Msrb2	Methionine-R-sulfoxide reductase B2, mitochondrial	2	2	1	125.4	Novel	HECT_2, Ribosomal_L37e, SelR, Yippee-Mis18, zinc_ribbon_10
`ENSMUSG00000026207`	Speg	Striated muscle-specific serine/threonine-protein kinase	2	2	1	41.8	Ref	ABC1, APH, C2-set_2, I-set, Ig_2, Ig_3, Ig_4, Ig_6, Izumo-Ig, Kdo, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Pox_ser-thr_kin, Receptor_2B4, SPEG_u2, V-set, fn3, ig
`ENSMUSG00000075704`	Txnrd2	Thioredoxin reductase 2, mitochondrial	3	2	1	26.4	Ref	AlaDh_PNT_C, DAO, FAD_binding_2, FAD_oxidored, GIDA, HI0933_like, Lys_Orn_oxgnase, NAD_binding_8, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Pyr_redox_dim, Thi4
`ENSMUSG00000025421`	Hdhd2	Haloacid dehalogenase-like hydrolase domain-containing protein 2	5	2	1	103.4	Ref	Acid_phosphat_B, DUF3155, HAD_2, Hydrolase, Hydrolase_6, Hydrolase_like
`ENSMUSG00000041199`	Rpusd1	RNA pseudouridylate synthase domain-containing protein 1	4	2	1	103.4	Ref	PseudoU_synth_2
`ENSMUSG00000027805`	PFN2	Profilin-2	2	2	1	578.7	Ref	DUF2076, DUF4106, Profilin
`ENSMUSG00000042389`	Tsen2	tRNA-splicing endonuclease subunit Sen2	2	2	1	4.4	Ref	FLYWCH_u, tRNA_int_endo, tRNA_int_endo_N
`ENSMUSG00000031878`	Nae1	NEDD8-activating enzyme E1 regulatory subunit	2	2	1	44.0	Ref	E1_4HB, SEP, ThiF
`ENSMUSG00000028180`	ZRANB2	Zinc finger Ran-binding domain-containing protein 2	2	2	1	17.6	Ref	DUF35_N, zf-RanBP
`ENSMUSG00000002343`	Armc6	Armadillo repeat-containing protein 6	2	2	1	52.8	Novel	Arm, Atx10homo_assoc, CRS1_YhbY, ELMO_ARM, Mis14, UBA_3
`ENSMUSG00000020598`	Nrcam	Neuronal cell adhesion molecule	2	2	1	24.2	Ref	Bravo_FIGEY, C2-set, C2-set_2, I-set, Ig_2, Ig_3, Ig_4, Ig_5, Ig_C17orf99, Interfer-bind, Izumo-Ig, Pur_ac_phosph_N, SIT, Syndecan, V-set, V-set_CD47, fn3, fn3_2, ig
`ENSMUSG00000032356`	Rasgrf1	Ras-specific guanine nucleotide-releasing factor 1	2	2	1	140.8	Ref	AAA_11, DUF3877, IQ, PH, PH_11, PH_13, PH_17, PH_20, RasGEF, RasGEF_N, RhoGEF
`ENSMUSG00000025264`	Tsr2	Pre-rRNA-processing protein TSR2 homolog	2	2	1	11.0	Ref	DUF6279, LPD22, WGG
`ENSMUSG00000033595`	Lgi3	Leucine-rich repeat LGI family member 3	2	2	1	48.4	Ref	EPTP, LRRCT, LRR_1, LRR_4, LRR_5, LRR_8, LRR_9, RCDG1, stn_TNFRSF12A
`ENSMUSG00000053799`	Exoc6	Exocyst complex component 6	2	2	1	6.6	Ref	AKAP7_NLS, COG5, DUF5856, Sec15
`ENSMUSG00000031347`	Cetn2	Centrin-2	2	2	1	37.4	Ref	Asp-B-Hydro_N, CNNM, Caleosin, DUF4601, DUF6694, Dockerin_1, EF-hand_1, EF-hand_11, EF-hand_14, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, Fin, HerA_C, LETM1_RBD, RTP, SRI, SUB1_ProdP9, SurA_N_2, SurA_N_3
`ENSMUSG00000047423`	Bles03	UPF0696 protein C11orf68 homolog	2	2	1	55.0	Ref	DUF1917, K_channel_TID
`ENSMUSG00000030401`	Rtn2	Reticulon-2	2	2	1	66.0	Ref	Babuvirus_MP, DUF592, Reticulon, mono-CXXC
`ENSMUSG00000035297`	Cops4	COP9 signalosome complex subunit 4	3	2	1	213.4	Novel	CSN4_RPN5_eIF3a, DinB, PCI, RPN7, TPR_12, TPR_14, TPR_7, TPR_8, YabA
`ENSMUSG00000025534`	Gusb	Beta-glucuronidase	2	2	1	13.2	Ref	Glyco_hydro_2, Glyco_hydro_2_C, Glyco_hydro_2_N
`ENSMUSG00000021647`	Cartpt	Cocaine- and amphetamine-regulated transcript protein	2	2	1	8.8	Ref	CART, DUF2114, DUF713, Opy2, Spore_Cse60
`ENSMUSG00000023973`	Cnpy3	Protein canopy homolog 3	2	2	1	101.2	Ref	DUF3456, SapB_1, TBK1_CCD1
`ENSMUSG00000024805`	Pcgf5	Polycomb group RING finger protein 5	2	2	1	6.6	Ref	DUF1272, DZR, DZR_2, E3_UbLigase_R4, G10, Prok-RING_4, RAWUL, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-C3HC4_5, zf-Nse, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX, zf-rbx1, zinc-ribbons_6
`ENSMUSG00000063445`	Nmral1	NmrA-like family domain-containing protein 1	2	2	1	41.8	Ref	3Beta_HSD, BRK, Epimerase, F420_oxidored, HTH_3, Helo_like_N, KR, NAD_binding_10, NmrA, Semialdhyde_dh, Shikimate_DH, TrkA_N, adh_short
`ENSMUSG00000029780`	Nt5c3a	Cytosolic 5'-nucleotidase 3A	2	2	1	55.0	Ref	ACT_7, CIDE-N, DZR_2, HAD, Hydrolase, UMPH-1, zf-ANAPC11
`ENSMUSG00000029125`	Stx18	Syntaxin-18	2	2	1	15.4	Ref	DUF6279, Phage_CP76, SR1P, Syntaxin-18_N, TipAS
`ENSMUSG00000024845`	Tmem134	Transmembrane protein 134	4	2	1	79.2	Ref	TMEM_230_134
`ENSMUSG00000034757`	Tmub2	Transmembrane and ubiquitin-like domain-containing protein 2	3	2	1	57.2	Ref	CD52, DUF6232, Dsc3_N, Rad60-SLD_2, ubiquitin
`ENSMUSG00000022702`	Hira	Protein HIRA	2	2	1	11.0	Ref	ANAPC4_WD40, HIRA_B, Hira, Mcl1_mid, NBCH_WD40, STAC2_u1, WD40, WD40_like
`ENSMUSG00000036564`	ndrg4	Protein NDRG4	3	2	1	143.0	Ref	Abhydrolase_1, Endotoxin_N, Ndr
`ENSMUSG00000037902`	Sirpa	Tyrosine-protein phosphatase non-receptor type substrate 1	2	2	1	15.4	Ref	C1-set, C2-set_2, DUF6479, I-set, Ig_2, Ig_3, MAT1-1-2, SIT, Ureide_permease, V-set, ig
`ENSMUSG00000017421`	Znf207	BUB3-interacting and GLEBS motif-containing protein ZNF207	2	2	1	17.6	Ref	SSP160, zf-C2H2, zf-C2H2_4, zf-FCS
`ENSMUSG00000030774`	Pak1	Serine/threonine-protein kinase PAK 1	3	2	1	116.6	Novel	ABC1, APH, DSHCT, DUF3741, Kdo, Kinase-like, PBD, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal
`ENSMUSG00000029408`	Abcb9	ABC-type oligopeptide transporter ABCB9	2	2	1	4.4	Ref	AAA_16, AAA_22, AAA_23, AAA_29, AAA_30, ABC_membrane, ABC_membrane_3, ABC_tran, ATP_bind_1, DUF1475, DUF4191, MCU, RsgA_GTPase, SMC_N, SbcC_Walker_B
`ENSMUSG00000037788`	Vopp1	WW domain binding protein VOPP1	2	2	1	50.6	Ref	Conotoxin_I2, FixQ, TMEM52, WBP-1, zf-His_Me_endon
`ENSMUSG00000038240`	Pdss2	All trans-polyprenyl-diphosphate synthase PDSS2	2	2	1	15.4	Novel	hnRNP_Q_AcD, polyprenyl_synt
`ENSMUSG00000045867`	Cradd	Death domain-containing protein CRADD	2	2	1	15.4	Ref	CARD, Death, Gam, TadE, Tmemb_18A
`ENSMUSG00000038195`	Rilp	Rab-interacting lysosomal protein	2	2	1	4.4	Ref	DUF6215, GSK-3_bind, Jnk-SapK_ap_N, Macoilin, RILP, V_ATPase_I
`ENSMUSG00000054934`	Kcnmb4	Calcium-activated potassium channel subunit beta-4	2	2	1	110.0	Novel	CaKB, LapA_dom
`ENSMUSG00000078941`	Ak6	Adenylate kinase isoenzyme 6	3	2	1	195.8	Ref	Bromo_TP, CENP-S, CENP-T_C, DUF2125, DUF3245, DUF4611, Histone, LonB_AAA-LID, TFIID-31kDa
`ENSMUSG00000030824`	Nucb1	Nucleobindin-1	2	2	1	19.8	Ref	EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, RNase_Y_N
`ENSMUSG00000079557`	Marchf2	E3 ubiquitin-protein ligase MARCHF2	4	2	1	81.4	Mixed	Baculo_IE-1, RINGv, zf-C3HC4, zf-RING_2, zf-rbx1
`ENSMUSG00000005069`	Pex5	Peroxisomal targeting signal 1 receptor	2	2	1	11.0	Ref	AAA_lid_7, ABATE, ANAPC3, DUF1641, Nsp1_C, PCRF, PPTA, SHNi-TPR, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_20, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000031068`	Glrx3	Glutaredoxin-3	2	2	1	8.8	Novel	Arf, CNOT1_HEAT_N, DIM1, DUF4279, DUF953, GREB1_C, GST_N_3, GerD, Glutaredoxin, HeH, HyaE, OST3_OST6, Phosducin, RdRP, SH3BGR, TMF_TATA_bd, Thioredoxin, Thioredoxin_2, Thioredoxin_8, Thioredoxin_9, TrbC_Ftype, ZinT
`ENSMUSG00000031533`	Mrps31	Small ribosomal subunit protein mS31	3	2	1	11.0	Novel	FANC_SAP, MRP-S31
`ENSMUSG00000059810`	Rgs3	Regulator of G-protein signaling 3	2	2	1	8.8	Novel	DUF4441, RGS
`ENSMUSG00000027935`	Rab13	Ras-related protein Rab-13	2	2	1	6.6	Ref	AAA, AAA_16, AAA_22, AAA_25, AAA_33, AAA_7, ATP_bind_3, Arf, GTP_EFTU, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, RuvX, SRPRB, Septin, TMP-TENI, TniB
`ENSMUSG00000020111`	Micu1	Calcium uptake protein 1, mitochondrial	3	2	1	57.2	Novel	Acyl-thio_N, DUF6559, Dockerin_1, EF-hand_1, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8
`ENSMUSG00000026254`	Eif4e2	Eukaryotic translation initiation factor 4E type 2	5	2	1	26.4	Ref	FlgD, IF4E, IHABP4_N, Stm1_N
`ENSMUSG00000004187`	Kifc2	Kinesin-like protein KIFC2	2	2	1	26.4	Ref	Baculo_PEP_C, DUF498, DUF6779, Fib_alpha, Kinesin, Microtub_bd, PIF1, Rubis-subs-bind, TACC_C, THOC7, YabA
`ENSMUSG00000078695`	Cisd3	CDGSH iron-sulfur domain-containing protein 3, mitochondrial	3	2	1	134.2	Ref	Inhibitor_I71, Toxin_14, zf-CDGSH
`ENSMUSG00000021773`	Comtd1	Catechol O-methyltransferase domain-containing protein 1	2	2	1	8.8	Ref	DNA_binding_1, MTS, Methyltransf_18, Methyltransf_24, Methyltransf_25, Methyltransf_3, Methyltransf_31, PCMT
`ENSMUSG00000026179`	Pnkd	Probable thioesterase PNKD	3	2	1	184.8	Ref	HAGH_C, Lactamase_B, Lactamase_B_2, Lactamase_B_3
`ENSMUSG00000019948`	Actr6	Actin-related protein 6	2	2	1	37.4	Novel	Actin, EcoRII-C, MreB_Mbl
`ENSMUSG00000028423`	Nfx1	Transcriptional repressor NF-X1	2	2	1	6.6	Ref	IP_trans, R3H, zf-NF-X1
`ENSMUSG00000036932`	Aifm1	Apoptosis-inducing factor 1, mitochondrial	2	2	1	19.8	Ref	AIF-MLS, AIF_C, Amino_oxidase, DUF3918, FAD_oxidored, HI0933_like, Lys_Orn_oxgnase, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Reductase_C, TPP_enzyme_M, Trp_halogenase
`ENSMUSG00000048967`	Yjefn3	YjeF N-terminal domain-containing protein 3	2	2	1	37.4	Novel	DUF4381, GRIM-19, Planc_extracel, YjeF_N
`ENSMUSG00000047473`	Zfp30	Zinc finger protein 30	2	2	1	0.0	Ref	BHD_1, C1_4, DNA_RNApol_7kD, DUF3850, DUF5830, KRAB, TFIIS_C, XPA_N, Zn-ribbon_8, zf-C2H2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000007812`	Znf655	Zinc finger protein 655	2	2	1	4.4	Ref	KRAB, Oxidored_q4, PHD, XPA_N, Zn-ribbon_8, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HC_2, zf-H2C2_2, zf_C2H2_13
`ENSMUSG00000024194`	Cuta	Protein CutA	7	2	1	200.2	Mixed	CutA1, DAHP_synth_1
`ENSMUSG00000031948`	Kars1	Lysine--tRNA ligase	2	2	1	52.8	Ref	DUF4407, HSP70, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon
`ENSMUSG00000064264`	Znf428	Zinc finger protein 428	2	2	1	114.4	Ref	PIEZO, zf-C2H2_4
`ENSMUSG00000056121`	Fez2	Fasciculation and elongation protein zeta-2	2	2	1	33.0	Ref	DUF5102, FEZ, FapA, Gluconate_2-dh3, MRP-L46, NPV_P10, V_ATPase_I
`ENSMUSG00000038957`	Edc3	Enhancer of mRNA-decapping protein 3	2	2	1	0.0	Ref	DUF5096, Edc3_linker, FDF, LSM14, YjeF_N
`ENSMUSG00000090015`	Znf431	Zinc finger protein 431	3	2	1	2.2	Ref	ATR13, BHD_1, La, PT-TG, RAD51_interact, RIH_assoc, UPF0370, Vps39_2
`ENSMUSG00000000827`	Tpd52l2	Tumor protein D54	3	2	1	8.8	Novel	HIP1_clath_bdg, TPD52, VASP_tetra
`ENSMUSG00000042271`	Nxt2	NTF2-related export protein 2	2	2	1	28.6	Ref	NTF2, QRPTase_N
`ENSMUSG00000061298`	Agbl4	Cytosolic carboxypeptidase 6	3	2	1	8.8	Novel	Pepdidase_M14_N, Peptidase_M14
`ENSMUSG00000030083`	Abtb1	Ankyrin repeat and BTB/POZ domain-containing protein 1	3	2	1	41.8	Mixed	BACK, BTB
`ENSMUSG00000023147`	Get1	Guided entry of tail-anchored proteins factor 1	2	2	1	26.4	Ref	ACP_PD, CHD5, DUF1043, DUF2203, DUF2868, DUF4781, DUF6376, DUF6674, MCU, Phlebovirus_NSM, Snapin_Pallidin
`ENSMUSG00000031885`	Cbfb	Core-binding factor subunit beta	2	2	1	4.4	Ref	CBF_beta, DUF6101
`ENSMUSG00000071655`	Ubxn1	UBX domain-containing protein 1	3	2	1	319.0	Ref	CDC45, Presenilin, Selenoprotein_S, UBA, UBX, eIF3_subunit
`ENSMUSG00000022771`	Ppil2	RING-type E3 ubiquitin-protein ligase PPIL2	3	2	1	11.0	Novel	Pro_isomerase, Rtf2, SUB1_ProdP9, U-box, zf-NOSIP
`ENSMUSG00000000308`	Ckmt1	Creatine kinase U-type, mitochondrial	2	2	1	376.2	Novel	ATP-gua_Ptrans, ATP-gua_PtransN
`ENSMUSG00000025270`	Alas2	5-aminolevulinate synthase, erythroid-specific, mitochondrial	2	2	1	13.2	Ref	Aminotran_1_2, Aminotran_5, Cys_Met_Meta_PP, DegT_DnrJ_EryC1, Mustang, Preseq_ALAS
`ENSMUSG00000059796`	EIF4A1	Eukaryotic initiation factor 4A-I	2	2	1	470.8	Ref	AAA_19, AAA_22, AAA_30, CDC37_C, DEAD, Flavi_DEAD, Helicase_C, Helicase_RecD, PhoH, ResIII, UvrD-helicase, tRNA-synt_1b
`ENSMUSG00000025968`	Ndufs1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial	2	2	1	118.8	Ref	Fer2, Fer2_4, Fer4, Molybdopterin, NADH-G_4Fe-4S_3, NADH_dhqG_C, TPP_enzyme_M
`ENSMUSG00000020282`	Rhbdf1	Inactive rhomboid protein 1	2	2	1	8.8	Ref	CoA_binding_3, DUF3298, Rhomboid, Rhomboid_SP
`ENSMUSG00000049600`	Zbtb45	Zinc finger and BTB domain-containing protein 45	2	2	1	30.8	Ref	BTB, DUF2256, HypA, LIM, Zn-ribbon_8, Zn_Tnp_IS1595, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-C2HC_2, zf-Di19, zf-H2C2_2, zf-UBP, zf-met, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000060703`	Cd302	CD302 antigen	2	2	1	169.4	Ref	DUF973, IN_DBD_C, Lectin_C, UL45
`ENSMUSG00000023192`	Grm2	Metabotropic glutamate receptor 2	2	2	1	4.4	Novel	7tm_3, ANF_receptor, DUF543, Gaa1, MAR_sialic_bdg, NCD3G
`ENSMUSG00000013523`	Bcas1	Breast carcinoma-amplified sequence 1 homolog	4	2	1	24.2	Novel	SelK_SelG
`ENSMUSG00000031781`	Ciapin1	Anamorsin	3	2	1	22.0	Novel	BBS2_C, CIAPIN1, Methyltransf_11, Methyltransf_12, Methyltransf_23, Methyltransf_25, Methyltransf_29, Methyltransf_31, Tat
`ENSMUSG00000047547`	Cltb	Clathrin light chain B	2	2	1	259.6	Ref	CENP-Q, Clathrin_lg_ch, DUF6161, GED
`ENSMUSG00000032497`	Lrrfip2	Leucine-rich repeat flightless-interacting protein 2	2	2	1	0.0	Novel	AAA_13, BicD, CALCOCO1, CCDC22, Crescentin, Csm1_N, DUF16, DUF1664, DUF2935, DUF724, EzrA, Fez1, GldM_N, HALZ, KASH_CCD, LRRFIP, NPV_P10, Phage_GP20, Syntaxin-6_N, TEDC1, TFA2_Winged_2, TPR_MLP1_2
`ENSMUSG00000028034`	Fubp1	Far upstream element-binding protein 1	2	2	1	30.8	Ref	DUF1897, KH_1, KH_2, KH_4, KH_5, NAD_kinase_C, SH3_12, Secretin_N
`ENSMUSG00000052926`	Rnaseh2a	Ribonuclease H2 subunit A	3	2	1	57.2	Ref	ALG11_N, RNase_HII
`ENSMUSG00000024797`	Vps51	Vacuolar protein sorting-associated protein 51 homolog	2	2	1	11.0	Ref	COG2, COG5, DUF3829, Dor1, GIT_CC, Occludin_ELL, Sec5, TMTC_DUF1736, Vps51, Vps53_N, Vps54_N, Zw10
`ENSMUSG00000027519`	Rab22a	Ras-related protein Rab-22A	2	2	1	4.4	Ref	AAA_16, AAA_5, AAA_7, Arf, CNTF, GTP_EFTU, Gtr1_RagA, MMR_HSR1, Maelstrom, NTPase_1, Ras, Roc, RsgA_GTPase, SpoIVA_ATPase
`ENSMUSG00000060450`	Rnf14	E3 ubiquitin-protein ligase RNF14	2	2	1	90.2	Ref	IBR, RWD, TBPIP, zf-C3HC4, zf-RING_11, zf-RING_2, zf-RING_5
`ENSMUSG00000056820`	Tsnax	Translin-associated protein X	2	2	1	246.4	Novel	DUF6291, PH_16, Phage_GPO, Translin
`ENSMUSG00000061559`	Skic8	Superkiller complex protein 8	3	2	1	143.0	Ref	ANAPC4_WD40, Cytochrom_D1, DUF2415, GSDH, Ge1_WD40, NBCH_WD40, Nucleoporin_N, Nup160, PD40, WD40, WD40_like, eIF2A
`ENSMUSG00000024826`	Dpf2	Zinc finger protein ubi-d4	2	2	1	24.2	Ref	C1_1, DPF1-3_N, Lar_restr_allev, PHD, PHD_2, PHD_4, zf-C2H2, zf-C2H2_4, zf-H2C2_2, zf-PHD-like
`ENSMUSG00000011267`	Znf296	Zinc finger protein 296	2	2	1	17.6	Novel	AFOR_C, DUF1390, DUF3268, GAGA, OrsD, Salp15, zf-BED, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-C2H2_6, zf-C2H2_7, zf-C2H2_jaz, zf-C2HC_2, zf-H2C2_2, zf-H2C2_5, zf-met, zinc_ribbon_15
`ENSMUSG00000073424`	Cyp4f4	Cytochrome P450 4F4	3	2	1	28.6	Novel	p450
`ENSMUSG00000029471`	Camkk2	Calcium/calmodulin-dependent protein kinase kinase 2	2	2	1	33.0	Ref	ABC1, APH, DUF4010, HAND, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, Seadorna_VP7
`ENSMUSG00000028862`	Map3k6	Mitogen-activated protein kinase kinase kinase 6	2	2	1	17.6	Ref	ASK_PH, DRHyd-ASK, Glu-tRNAGln, HisK-N-like, MAP3K_TRAF_bd, NTP_transf_5, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SAM_KSR1, TPR-S, YrbL-PhoP_reg
`ENSMUSG00000041278`	Ttc1	Tetratricopeptide repeat protein 1	2	2	1	189.2	Ref	MG2, RPN7, SOAR, TPR_1, TPR_11, TPR_12, TPR_14, TPR_15, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9, eIF3_N
`ENSMUSG00000069255`	Dusp22	Dual specificity protein phosphatase 22	2	2	1	17.6	Ref	DSPc, EZH2_N, Init_tRNA_PT, PTPlike_phytase, Y_phosphatase
`ENSMUSG00000003549`	Ercc1	DNA excision repair protein ERCC-1	2	2	1	72.6	Ref	HHH, HHH_2, HHH_5, Rad10, UPF0758_N
`ENSMUSG00000001964`	Emd	Emerin	2	2	1	1,925.2	Ref	DUF2729, LEM, SUI1
`ENSMUSG00000029128`	Rab28	Ras-related protein Rab-28	2	2	1	96.8	Ref	Arf, DLIC, G-alpha, Gtr1_RagA, PduV-EutP, Ras, Roc, WHAMM-JMY_N, XFP_N
`ENSMUSG00000029319`	Coq2	4-hydroxybenzoate polyprenyltransferase, mitochondrial	2	2	1	57.2	Novel	DUF2798, UbiA
`ENSMUSG00000041912`	Tdrkh	Tudor and KH domain-containing protein	2	2	1	22.0	Ref	KH_1, KH_2, KH_4, KH_5, MOEP19, Ribosomal_S12, TUDOR
`ENSMUSG00000027223`	Mapk8ip1	C-Jun-amino-terminal kinase-interacting protein 1	2	2	1	11.0	Ref	PID, PID_2, SH3_1, SH3_2, SH3_9, Tam41_Mmp37
`ENSMUSG00000021071`	Trim9	E3 ubiquitin-protein ligase TRIM9	3	2	1	52.8	Ref	DUF2203, Prok-RING_4, Trm112p, fn3, zf-B_box, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000032305`	Fam219b	Protein FAM219B	2	2	1	0.0	Ref	DUF6749, FAM219A
`ENSMUSG00000052584`	SERP2	Stress-associated endoplasmic reticulum protein 2	3	2	1	250.8	Novel	DUF1206, RAMP4
`ENSMUSG00000052337`	Immt	MICOS complex subunit Mic60	3	2	1	48.4	Ref	Mitofilin
`ENSMUSG00000021916`	Glt8d1	Glycosyltransferase 8 domain-containing protein 1	2	2	1	11.0	Ref	Glyco_transf_24, Glyco_transf_8, Trehalose_recp
`ENSMUSG00000041840`	Haus1	HAUS augmin-like complex subunit 1	2	2	1	17.6	Ref	DUF4407, SKA1, Streptin-Immun, THP2, XhlA
`ENSMUSG00000040746`	Rnf167	E3 ubiquitin-protein ligase RNF167	3	2	1	107.8	Mixed	CTU2, FAM176, FANCL_C, PHD, Prok-RING_1, Prok-RING_4, RINGv, RecR, zf-ANAPC11, zf-C3H2C3, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-RING-like, zf-RING_11, zf-RING_15, zf-RING_16, zf-RING_2, zf-RING_4, zf-RING_5, zf-RING_UBOX, zf-UDP, zf-rbx1
`ENSMUSG00000027953`	Slc50a1	Sugar transporter SWEET1	3	2	1	112.2	Mixed	MtN3_slv, PQ-loop, YhhN
`ENSMUSG00000024539`	Ptpn2	Tyrosine-protein phosphatase non-receptor type 2	2	2	1	24.2	Ref	CHASE3, DSPc, RIC3, Y_phosphatase, Y_phosphatase3
`ENSMUSG00000062822`	—	Uncharacterized protein C5orf34 homolog	2	2	1	2.2	Ref	DUF4520, DUF4524, Lipocalin
`ENSMUSG00000026767`	Mindy3	Ubiquitin carboxyl-terminal hydrolase MINDY-3	6	2	1	11.0	Mixed	EF-hand_7, MINDY-3_4_CD
`ENSMUSG00000015749`	Anp32e	Acidic leucine-rich nuclear phosphoprotein 32 family member E	2	2	1	44.0	Ref	BUD22, FBXL18_LRR, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9, SAPS
`ENSMUSG00000020755`	Sap30bp	SAP30-binding protein	2	2	1	15.4	Novel	CCDC84, HCNGP, Rib_recp_KP_reg
`ENSMUSG00000034563`	Ccpg1	Cell cycle progression protein 1	3	2	1	35.2	Ref	Csm1_N, DHR-2_Lobe_C, YtxH
`ENSMUSG00000107068`	Pagr1a	PAXIP1-associated glutamate-rich protein 1A	2	2	1	112.2	Novel	CD225, Nop53, PAXIP1_C
`ENSMUSG00000020458`	Rtn4	Reticulon-4	2	2	1	422.4	Novel	DUF4736, DUF639, Phage_holin_3_6, Reticulon
`ENSMUSG00000006519`	Cyba	Cytochrome b-245 light chain	2	2	1	26.4	Ref	COPI_assoc, Cg6151-P, Cytochrom_B558a, DUF6030, SdpI, TgpA_N
`ENSMUSG00000021692`	Dimt1	Dimethyladenosine transferase	2	2	1	6.6	Ref	CMAS, DOT1, HHH_4, MTS, Met_10, Methyltransf_11, Methyltransf_12, Methyltransf_18, Methyltransf_2, Methyltransf_23, Methyltransf_25, PCMT, R3H-assoc, RrnaAD, Ubie_methyltran
`ENSMUSG00000002660`	Clpp	ATP-dependent Clp protease proteolytic subunit, mitochondrial	2	2	1	266.2	Novel	CLP_protease, MDMPI_C, YgaB
`ENSMUSG00000024037`	Wdr4	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4	2	2	1	8.8	Ref	Nup160, WD40
`ENSMUSG00000026201`	Stk16	Serine/threonine-protein kinase 16	4	2	1	110.0	Novel	ABC1, APH, Kdo, Kinase-like, PK_Tyr_Ser-Thr, Pkinase, TCAD9
`ENSMUSG00000020585`	Laptm4a	Lysosomal-associated transmembrane protein 4A	2	2	1	688.7	Novel	Circovir2_Orf4, DUF4728, Mtp
`ENSMUSG00000033099`	Nol12	Nucleolar protein 12	2	2	1	15.4	Novel	ALC, FKBP_N, Nop25, Nup54_C
`ENSMUSG00000047342`	ZNF286A	Zinc finger protein 286A	2	2	1	13.2	Ref	C1_4, DNA_RNApol_7kD, DUF5830, DUF6076, DZR, HVO_2753_ZBP, HalOD2, KRAB, LIM, OSTMP1, TFIIS_C, XPA_N, Zn-ribbon_8, Zn_ribbon_SprT, Zn_ribbon_recom, zf-AN1, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-LYAR, zf-met, zf-trcl, zf_UBZ, zinc_ribbon_15, zinc_ribbon_9
`ENSMUSG00000055447`	Cd47	Leukocyte surface antigen CD47	4	2	1	79.2	Ref	CD47, Ig_3, Mpo1-like, TssN, V-set_CD47
`ENSMUSG00000020903`	Stx8	Syntaxin-8	2	2	1	30.8	Ref	DUF1731, DUF2095, Otopetrin, Prominin, SNARE, Sgf11, TFIIS_M, Use1, zf-ANAPC11, zf_C2HC_14
`ENSMUSG00000037279`	Ovol2	Transcription factor Ovo-like 2	2	2	1	2.2	Novel	A2L_zn_ribbon, ADK_lid, C1_1, C5HCH, FYVE, FpoO, HEPN_RES_NTD1, Rubredoxin_2, XPA_N, YjdM_Zn_Ribbon, Zn-ribbon_8, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_3rep, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-H2C2_5, zf-MYST, zf-met, zf_C2H2_ZHX, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000019809`	Pex3	Peroxisomal biogenesis factor 3	2	2	1	22.0	Ref	DUF6672, Peroxin-3
`ENSMUSG00000006392`	Med8	Mediator of RNA polymerase II transcription subunit 8	2	2	1	92.4	Ref	Baculo_PEP_C, CENP-Q, CSN4_RPN5_eIF3a, Med8, SlyX
`ENSMUSG00000024118`	Tedc2	Tubulin epsilon and delta complex protein 2	2	2	1	6.6	Ref	DUF1633, DUF4693
`ENSMUSG00000007987`	Ift22	Intraflagellar transport protein 22 homolog	2	2	1	33.0	Ref	AAA, Arf, FMN_red, G-alpha, GTP_EFTU, Gtr1_RagA, MMR_HSR1, PduV-EutP, Ras, Roc, SRPRB
`ENSMUSG00000030881`	Arfip2	Arfaptin-2	3	2	1	50.6	Ref	Arfaptin, BAR, BAR_3, Blo-t-5, TPR_MLP1_2
`ENSMUSG00000035278`	Plekhj1	Pleckstrin homology domain-containing family J member 1	2	2	1	173.8	Novel	PH, PH_13, PH_16, Pinin_SDK_N
`ENSMUSG00000036242`	Armh4	Armadillo-like helical domain-containing protein 4	2	2	1	8.8	Ref	ARMH4, Autophagy_act_C, BUD22, CDC45, Csc2, DUF2428, FAM176, FYDLN_acid, MLANA, NOA36, Nop14, PI3K_1B_p101, RPA43_OB, Sigma70_ner, TFIIA, TRAP_alpha
`ENSMUSG00000074182`	Znhit6	Box C/D snoRNA protein 1	2	2	1	6.6	Ref	PolC_DP2, zf-B_box, zf-HIT
`ENSMUSG00000042675`	YPEL3	Protein yippee-like 3	3	2	1	459.8	Mixed	RIG-I_C-RD, Yippee-Mis18
`ENSMUSG00000029359`	Tesc	Calcineurin B homologous protein 3	3	2	1	257.4	Mixed	EF-hand_1, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, M16C_assoc
`ENSMUSG00000031516`	Dctn6	Dynactin subunit 6	2	2	1	336.6	Novel	Fucokinase, Hexapep
`ENSMUSG00000014767`	Tbp	TATA-box-binding protein	2	2	1	8.8	Ref	ANAPC_CDC26, AcnX_swivel_put, Pox_A6, Presenilin, TBP, TFIIA, Tombus_movement, zf-HIT, zf-MYND
`ENSMUSG00000035949`	Fbxw2	F-box/WD repeat-containing protein 2	3	2	1	30.8	Ref	ANAPC4_WD40, DUF5570, F-box, F-box-like, NBCH_WD40, WD40
`ENSMUSG00000042182`	Bend6	BEN domain-containing protein 6	2	2	1	41.8	Ref	BEN, CCDC-167, DUF5725, FlaC_arch, K-box, Prefoldin_2, TSC22, bZIP_2
`ENSMUSG00000027574`	Nkain4	Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4	3	2	1	160.6	Mixed	12TM_1, NKAIN
`ENSMUSG00000022617`	Chkb	Choline/ethanolamine kinase	2	2	1	48.4	Ref	APH, Choline_kinase, EcKL, Pkinase_fungal, T2SSL
`ENSMUSG00000021124`	Vti1b	Vesicle transport through interaction with t-SNAREs homolog 1B	2	2	1	72.6	Ref	4HB_MCP_1, CCDC-167, Cir_Bir_Yir, DASH_Duo1, DUF1759, DUF3376, DUF3958, DUF6245, DUF745, Exonuc_VII_L, Fusion_gly, KxDL, Laminin_II, OmpH, Sec20, Snapin_Pallidin, Syntaxin-6_N, UPF0449, V-SNARE, V-SNARE_C, YqjK
`ENSMUSG00000037706`	Cd81	CD81 antigen	2	2	1	1,386.1	Novel	DUF6768, Tetraspanin
`ENSMUSG00000015149`	Sirt2	NAD-dependent protein deacetylase sirtuin-2	3	2	1	250.8	Ref	RecQ_Zn_bind, SIR2, YdjO
`ENSMUSG00000056167`	Cnot10	CCR4-NOT transcription complex subunit 10	2	2	1	0.0	Ref	ANAPC3, BLOC1_2, DUF6058, DUF6078, DUF948, Dict-STAT-coil, NBD94, SRP_TPR_like, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_MalT, Uricase
`ENSMUSG00000063802`	Hspbp1	Hsp70-binding protein 1	4	2	1	220.0	Novel	Arm, CLIP_SPH_mas, Fes1, HEAT, HEAT_EZ, PPTA, Rcd1
`ENSMUSG00000018567`	GABARAP	Gamma-aminobutyric acid receptor-associated protein	2	2	1	946.1	Ref	APG12, ATG8, DUF6501, PRIMA1
`ENSMUSG00000029755`	Dlx5	Homeobox protein DLX-5	2	2	1	19.8	Ref	Cauli_DNA-bind, DLL_N, Homeobox_KN, Homeodomain, YdaS_antitoxin
`ENSMUSG00000038871`	Bpgm	Bisphosphoglycerate mutase	3	2	1	11.0	Novel	Exc, His_Phos_1
`ENSMUSG00000015488`	Cacfd1	Calcium channel flower homolog	3	2	1	22.0	Ref	Bacteriocin_IIc, COPI_assoc, Cg6151-P, TMEM72
`ENSMUSG00000033701`	Acbd6	Acyl-CoA-binding domain-containing protein 6	3	2	1	57.2	Ref	ACBP
`ENSMUSG00000035215`	Lsm7	U6 snRNA-associated Sm-like protein LSm7	2	2	1	132.0	Novel	COG7, ExoD, LSM
`ENSMUSG00000039419`	Cntnap2	Contactin-associated protein-like 2	2	2	1	35.2	Ref	Adeno_E3_CR2, COLFI, EGF, F5_F8_type_C, Laminin_G_1, Laminin_G_2, Laminin_G_3, MAP17, MARVEL, Syndecan, Tmemb_9, UstYa, hEGF
`ENSMUSG00000036819`	Jmjd4	2-oxoglutarate and iron-dependent oxygenase JMJD4	2	2	1	2.2	Ref	Cupin_8, DUF4314, JmjC, JmjC_2, SWIM
`ENSMUSG00000069171`	Nr2f1	COUP transcription factor 1	2	2	1	44.0	Novel	Hormone_recep, XS, zf-C4
`ENSMUSG00000041629`	VCF1	Protein VCF1	2	2	1	11.0	Novel	FAM104, QWRF
`ENSMUSG00000001127`	Araf	Serine/threonine-protein kinase A-Raf	5	2	1	204.6	Novel	C1_1, RBD, TUG-UBL1, zf-RING-like, zf-RING_2
`ENSMUSG00000037089`	Slc35b2	Adenosine 3'-phospho 5'-phosphosulfate transporter 1	2	2	1	61.6	Ref	DUF2070, EamA, PI3K_rbd, TPT, UAA
`ENSMUSG00000021578`	Lrrc14b	Leucine-rich repeat-containing protein 14B	2	2	1	19.8	Ref	ATG14, CT_C_D, CxC2, Exonuc_VII_L, FAP206, LRR_1, LRR_4, LRR_6, LRR_8, Mit_KHE1, Muted, PIN_3, TMPIT, Tuberin
`ENSMUSG00000030058`	Copg1	Coatomer subunit gamma-1	2	2	1	158.4	Ref	Adaptin_N, COP-gamma_platf, Cm_res_leader, Cnd1, Cnd3, Coatomer_g_Cpla, HEAT, HEAT_2, HEAT_EZ, HEAT_PBS, MOR2-PAG1_C, PDS5, UNC45-central
`ENSMUSG00000005699`	Pard6a	Partitioning defective 6 homolog alpha	6	2	1	61.6	Novel	PB1, PDZ, PDZ_6
`ENSMUSG00000029048`	Rer1	Protein RER1	2	2	1	85.8	Ref	DUF2208, Rer1, SEP
`ENSMUSG00000019139`	Isyna1	Inositol-3-phosphate synthase 1	2	2	1	46.2	Ref	Inos-1-P_synth, NAD_binding_5, UTP15_C
`ENSMUSG00000054766`	Set	Protein SET	2	2	1	39.6	Ref	DUF6279, EMP24_GP25L, Mst1_SARAH, NAP, Nore1-SARAH, OmpH, Phage_Mu_Gam, ZapB
`ENSMUSG00000030008`	Pradc1	Protease-associated domain-containing protein 1	2	2	1	28.6	Novel	PA, PRIMA1
`ENSMUSG00000035863`	Palm	Paralemmin-1	2	2	1	79.2	Ref	FeoB_Cyto, Paralemmin
`ENSMUSG00000022681`	Ntan1	Protein N-terminal asparagine amidohydrolase	8	2	1	103.4	Novel	FIST, N_Asn_amidohyd
`ENSMUSG00000029681`	Bcl7b	B-cell CLL/lymphoma 7 protein family member B	2	2	1	176.0	Novel	ANGEL2_N, BCL_N
`ENSMUSG00000043284`	Tmem11	Transmembrane protein 11, mitochondrial	3	2	1	147.4	Novel	Mito_morph_reg
`ENSMUSG00000022940`	Pigp	Phosphatidylinositol N-acetylglucosaminyltransferase subunit P	4	2	1	127.6	Ref	DPM2, PIG-P, Phage_holin_3_6, SieB
`ENSMUSG00000057236`	RBBP4	Histone-binding protein RBBP4	6	2	1	26.4	Mixed	ANAPC4_WD40, CAF1C_H4-bd, Capsid_NCLDV, NBCH_WD40, WD40, eIF2A
`ENSMUSG00000031622`	Sin3b	Paired amphipathic helix protein Sin3b	2	2	1	114.4	Ref	DUF5077, PAH, Sin3_corepress, Sin3a_C
`ENSMUSG00000020932`	Gfap	Glial fibrillary acidic protein	2	2	1	35.2	Ref	Apolipoprotein, BLOC1_2, CENP-F_leu_zip, DUF1664, DUF4407, Fib_alpha, Filament, Filament_head, GAS, HU-CCDC81_bac_2, Rsa3, Snu56_snRNP, TPR_MLP1_2
`ENSMUSG00000024160`	Spsb3	SPRY domain-containing SOCS box protein 3	4	2	1	88.0	Mixed	SOCS_box, SPRY
`ENSMUSG00000022111`	Uchl3	Ubiquitin carboxyl-terminal hydrolase isozyme L3	2	2	1	213.4	Novel	HpaB, Peptidase_C12
`ENSMUSG00000018160`	Med1	Mediator of RNA polymerase II transcription subunit 1	2	2	1	24.2	Ref	Med1, NIF3
`ENSMUSG00000078570`	—	Uncharacterized protein C1orf122 homolog	4	2	1	37.4	Novel	CCDC85, DUF4570, DUF4726
`ENSMUSG00000002846`	Timmdc1	Complex I assembly factor TIMMDC1, mitochondrial	2	2	1	44.0	Ref	Bacteriocin_IIc, DUF4748, Rick_17kDa_Anti, Tim17
`ENSMUSG00000018322`	Tomm34	Mitochondrial import receptor subunit TOM34	2	2	1	116.6	Ref	ANAPC3, BTAD, DUF4919, Fis1_TPR_C, MIT, PSII_Pbs27, SHNi-TPR, SRP_TPR_like, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_6, TPR_7, TPR_8, TPR_9, TPR_MalT
`ENSMUSG00000008301`	Phax	Phosphorylated adapter RNA export protein	2	2	1	24.2	Ref	DUF4692, PHAX_RNA-bd
`ENSMUSG00000019883`	Echdc1	Ethylmalonyl-CoA decarboxylase	2	2	1	4.4	Ref	DUF4478, ECH_1, ECH_2, Sp-DndD, WWE
`ENSMUSG00000006095`	Tbcb	Tubulin-folding cofactor B	2	2	1	409.2	Ref	CAP_GLY, RSV_NS2, Ubiquitin_2
`ENSMUSG00000028795`	Ccdc28b	Coiled-coil domain-containing protein 28B	2	2	1	55.0	Ref	CCDC28, DUF892
`ENSMUSG00000030605`	Mfge8	Lactadherin	2	2	1	305.8	Ref	EGF, EGF_2, F5_F8_type_C, Laminin_EGF, Muskelin_N, cEGF, hEGF
`ENSMUSG00000017679`	Ttpal	Alpha-tocopherol transfer protein-like	2	2	1	13.2	Ref	CRAL_TRIO, CRAL_TRIO_2, CRAL_TRIO_N
`ENSMUSG00000019906`	Lin7a	Protein lin-7 homolog A	2	2	1	4.4	Ref	7TMR-HDED, CENP-Q, CREPT, DUF932, L27, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Tricorn_PDZ
`ENSMUSG00000040289`	Hey1	Hairy/enhancer-of-split related with YRPW motif protein 1	2	2	1	81.4	Novel	HLH, HTH_Crp_2, Hairy_orange, dCache_2
`ENSMUSG00000048495`	Tyw5	tRNA wybutosine-synthesizing protein 5	3	2	1	26.4	Ref	Cupin_8, JmjC, JmjC_2
`ENSMUSG00000059824`	Dbp	D site-binding protein	2	2	1	15.4	Ref	BSD, bZIP_1, bZIP_2, bZIP_Maf
`ENSMUSG00000121472`	Setdb1	Histone-lysine N-methyltransferase SETDB1	2	2	1	4.4	Ref	B2, DUF6549, SseC, Tropomyosin_1
`ENSMUSG00000020289`	Nprl3	GATOR1 complex protein NPRL3	2	2	1	8.8	Ref	NPR2, NPR3, Pur_DNA_glyco
`ENSMUSG00000057469`	E2f6	Transcription factor E2F6	2	2	1	2.2	Ref	BRCA-2_OB3, DUF5719, DUF5908, DUF603, E2F_CC-MB, E2F_TDP, TrmB, vATP-synt_E
`ENSMUSG00000027397`	Slc20a1	Sodium-dependent phosphate transporter 1	2	2	1	63.8	Ref	DUF2269, PHO4
`ENSMUSG00000030086`	Chchd6	MICOS complex subunit Mic25	2	2	1	578.7	Ref	ATG14, Activator-TraM, CHCH, DUF1690, DUF3150, DUF4337, DUF6462, Exonuc_VII_L, FapA, IZUMO, MIC19_MIC25, NDUF_B7, hDGE_amylase
`ENSMUSG00000028647`	Mycbp	c-Myc-binding protein	2	2	1	6.6	Ref	CENP-F_N, TSC22, ZapB
`ENSMUSG00000068329`	Htra2	Serine protease HTRA2, mitochondrial	2	2	1	39.6	Ref	GRASP55_65, PDZ, PDZ_2, PDZ_6, Peptidase_M50, Peptidase_S32, Peptidase_S46, Trypsin, Trypsin_2
`ENSMUSG00000067873`	Htatsf1	17S U2 SnRNP complex component HTATSF1	2	2	1	39.6	Ref	Nup35_RRM, RRM_1, RRM_7
`ENSMUSG00000026854`	Usp20	Ubiquitin carboxyl-terminal hydrolase 20	2	2	1	2.2	Ref	C1_2, DUSP, UCH, UCH_1, zf-UBP, zinc_ribbon_9
`ENSMUSG00000063406`	Tmed5	Transmembrane emp24 domain-containing protein 5	2	2	1	11.0	Ref	DUF155, EMP24_GP25L
`ENSMUSG00000056962`	Jmjd6	Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6	2	2	1	13.2	Novel	AF-4, CCDC106, Cupin_8, IMUP, JmjC, JmjC_2, SR-25
`ENSMUSG00000027854`	Sike1	Suppressor of IKBKE 1	2	2	1	6.6	Novel	CorA, DUF4250, Herpes_BLRF2, SIKE, Spectrin, TSC22, TolA_bind_tri, YabA, betaPIX_CC
`ENSMUSG00000038695`	Josd2	Josephin-2	6	2	1	112.2	Mixed	Josephin
`ENSMUSG00000034868`	MYL12B	Myosin regulatory light chain 12B	2	2	1	231.0	Novel	2-Hacid_dh_C, DDE_Tnp_IS66_C, DUF2267, DUF2666, DUF3741, EF-hand_1, EF-hand_11, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, GPHH, SPARC_Ca_bdg, SurA_N_2, UPF0154
`ENSMUSG00000026096`	Osgepl1	tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial	2	2	1	19.8	Ref	BcrAD_BadFG, CheC, NADH_4Fe-4S, TsaD
`ENSMUSG00000028416`	Bag1	BAG family molecular chaperone regulator 1	2	2	1	92.4	Novel	BAG, BORCS6, DASH_Dad2, DUF2935, DUF4614, DUF5082, NPA, Tropomyosin_1, YbgS, ubiquitin
`ENSMUSG00000020925`	Ccdc43	Coiled-coil domain-containing protein 43	2	2	1	39.6	Ref	RepL, SPECT1, TetR_C_28
`ENSMUSG00000013495`	Tmem175	Endosomal/lysosomal proton channel TMEM175	3	2	1	13.2	Mixed	TMEM175
`ENSMUSG00000074165`	ZNF878	Zinc finger protein 878	2	2	1	11.0	Ref	C1_4, DUF4476, DUF968, KRAB, Malic_M, Zn-ribbon_8, Zn_ribbon_SprT, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_jaz, zf-H2C2_2, zf-trcl, zf_UBZ, zinc_ribbon_15
`ENSMUSG00000004633`	CHN2	Beta-chimaerin	2	2	1	19.8	Ref	C1_1, FYVE_2, RhoGAP, SH2, zf-RING-like
`ENSMUSG00000027333`	Smox	Spermine oxidase	2	2	1	33.0	Ref	3HCDH_N, AlaDh_PNT_C, Amino_oxidase, DAO, FAD_binding_2, FAD_binding_3, FAD_oxidored, FMO-like, GIDA, Glu_dehyd_C, HI0933_like, Lycopene_cycl, MCRA, MLTD_N, NAD_binding_8, NAD_binding_9, Pyr_redox, Pyr_redox_2, Pyr_redox_3, Shikimate_DH, Thi4, Trp_halogenase
`ENSMUSG00000028163`	Nfkb1	Nuclear factor NF-kappa-B p105 subunit	2	2	1	11.0	Ref	Ank, Ank_2, Ank_3, Ank_4, Ank_5, Death, Gag_p19, Keratin_2_head, RHD_DNA_bind, RHD_dimer
`ENSMUSG00000038880`	Mrps34	Small ribosomal subunit protein mS34	2	2	1	226.6	Ref	MRP-S34, NDK, PSI_PsaJ, Peptidase_S77
`ENSMUSG00000022749`	Tbc1d23	TBC1 domain family member 23	2	2	1	8.8	Ref	RabGAP-TBC, Rhodanese, TBC1D23_C
`ENSMUSG00000027088`	Phospho2	Pyridoxal phosphate phosphatase PHOSPHO2	2	2	1	13.2	Ref	HAD, HAD_2, Hydrolase, MBDa, Put_Phosphatase, RRM_1, RRM_7, RRM_occluded
`ENSMUSG00000032186`	Tmod2	Tropomodulin-2	2	2	1	24.2	Ref	LRR_6, Peptidase_M3_N, Tropomodulin
`ENSMUSG00000053768`	Chchd3	MICOS complex subunit Mic19	2	2	1	83.6	Novel	CHCH, DUF1690, DUF885, ECH_2, Fes1, MIC19_MIC25, NDUF_B7
`ENSMUSG00000059743`	Fdps	Farnesyl pyrophosphate synthase	2	2	1	286.0	Ref	ANIS5_cation-bd, polyprenyl_synt
`ENSMUSG00000063765`	Chadl	Chondroadherin-like protein	2	2	1	19.8	Ref	LRRCT, LRRNT, LRR_1, LRR_4, LRR_5, LRR_6, LRR_8, LRR_9
`ENSMUSG00000039217`	Il18	Interleukin-18	2	2	1	4.4	Novel	DUF273, IL1, NCA2
`ENSMUSG00000020070`	RUFY2	RUN and FYVE domain-containing protein 2	2	2	1	8.8	Ref	GIT_CC, HALZ, KASH_CCD, RUN, Shugoshin_N, Trimer_CC, Type_III_YscG, YabA, bZIP_1
`ENSMUSG00000026333`	Gin1	Gypsy retrotransposon integrase-like protein 1	2	2	1	4.4	Ref	Cytochrom_D1, Integrase_H2C2, rve, zf-H2C2
`ENSMUSG00000028152`	TSPAN5	Tetraspanin-5	2	2	1	127.6	Ref	DUF4378, Maf_N, Phage_holin_3_6, Tetraspanin
`ENSMUSG00000027642`	Rpn2	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2	3	2	1	39.6	Novel	DUF5859, HSM3_N, PI3K_rbd, Ribophorin_II
`ENSMUSG00000028657`	Ppt1	Palmitoyl-protein thioesterase 1	2	2	1	33.0	Ref	Abhydrolase_1, Palm_thioest, U71
`ENSMUSG00000033526`	Ppip5k1	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1	2	2	1	22.0	Ref	PPIP5K2_N, RimK
`ENSMUSG00000033735`	Spr	Sepiapterin reductase	2	2	1	187.0	Ref	KR, Nodulin-like, adh_short, adh_short_C2
`ENSMUSG00000027206`	COPS2	COP9 signalosome complex subunit 2	2	2	1	52.8	Ref	FliS, PCI, TPR_1, TPR_2, TPR_7, YozE_SAM_like
`ENSMUSG00000014195`	Dnajc7	DnaJ homolog subfamily C member 7	2	2	1	233.2	Ref	ANAPC3, Alkyl_sulf_dimr, BTAD, DnaJ, MIT, NFACT-R_1, RPN7, RPT, Rapsyn_N, SHNi-TPR, Sel1, TPR-S, TPR_1, TPR_10, TPR_11, TPR_12, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_3, TPR_4, TPR_6, TPR_7, TPR_8, TPR_9
`ENSMUSG00000024617`	CAMK2A	Calcium/calmodulin-dependent protein kinase type II subunit alpha	3	2	1	123.2	Novel	APH, CaMKII_AD, DUF4440, DUF6590, FTA2, Kdo, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, SnoaL_2, SnoaL_3, SnoaL_4
`ENSMUSG00000027286`	Lrrc57	Leucine-rich repeat-containing protein 57	4	2	1	44.0	Ref	CSTF_C, LRR_1, LRR_4, LRR_6, LRR_8, LRR_9
`ENSMUSG00000022223`	Sdr39u1	Epimerase family protein SDR39U1	2	2	1	143.0	Novel	DUF1731, Epimerase, GDP_Man_Dehyd, NAD_binding_10, Pyr_redox, RmlD_sub_bind
`ENSMUSG00000022634`	Yaf2	YY1-associated factor 2	3	2	1	26.4	Mixed	PROCN, RAP1, YAF2_RYBP, zf-RanBP
`ENSMUSG00000019782`	Rwdd1	RWD domain-containing protein 1	2	2	1	264.0	Novel	DFRP_C, DHHA1, Folliculin, Importin_rep_6, PARP, RWD
`ENSMUSG00000026797`	STXBP1	Syntaxin-binding protein 1	2	2	1	396.0	Ref	DUF5344, FRB_dom, Sec1, YlbD_coat
`ENSMUSG00000024369`	Nelfe	Negative elongation factor E	3	2	1	19.8	Ref	Nup35_RRM_2, RPA43_OB, RRM_1, RRM_occluded
`ENSMUSG00000060073`	Psma3	Proteasome subunit alpha type-3	5	2	1	646.9	Ref	Arm-DNA-bind_3, Proteasome
`ENSMUSG00000029310`	Nudt9	ADP-ribose pyrophosphatase, mitochondrial	3	2	1	101.2	Novel	NUDIX
`ENSMUSG00000035632`	Cnot3	CCR4-NOT transcription complex subunit 3	2	2	1	19.8	Ref	Cluap1, DUF2433, DUF4407, FlxA, Glyco_hydro_67C, NOT2_3_5, Not3, PilJ, Swi5
`ENSMUSG00000050471`	Fam118b	Protein FAM118B	2	2	1	2.2	Ref	DUF5443, SIR2_2
`ENSMUSG00000019738`	POLR2I	DNA-directed RNA polymerase II subunit RPB9	2	2	1	59.4	Ref	CpXC, DUF3716, DZR, Ogr_Delta, RNA_POL_M_15KD, TFIIS_C, TF_Zn_Ribbon, Zn-ribbon_8, Zn_Tnp_IS1595, Zn_Tnp_IS91, Zn_ribbon_recom
`ENSMUSG00000026270`	Capn10	Calpain-10	2	2	1	24.2	Ref	Calpain_III, Peptidase_C2
`ENSMUSG00000030016`	Znf638	Zinc finger protein 638	2	2	1	8.8	Novel	DUF5810, RRM_1, SF3a60_Prp9_C, zf-C2H2_jaz, zf-met
`ENSMUSG00000048076`	ARF1	ADP-ribosylation factor 1	2	2	1	657.9	Ref	6PF2K, Arf, DO-GTPase2, DUF1806, G-alpha, Gtr1_RagA, MMR_HSR1, Ras, Roc, SRPRB
`ENSMUSG00000030869`	Ndufab1	Acyl carrier protein, mitochondrial	3	2	1	55.0	Mixed	PP-binding, PP-binding_2
`ENSMUSG00000028545`	Bend5	BEN domain-containing protein 5	2	2	1	13.2	Ref	AAA_23, BEN, DASH_Spc34, DUF349, DUF5917, DUF6594, Docking, LMBR1, OmpH, PIN_12, Sec8_exocyst, Sipho_Gp157, T4SS, TSNAXIP1_N
`ENSMUSG00000018189`	Uchl5	Ubiquitin carboxyl-terminal hydrolase isozyme L5	2	2	1	28.6	Novel	BBP1_C, DUF2785, PNPase, Peptidase_C12, UCH_C
`ENSMUSG00000020718`	Polg2	DNA polymerase subunit gamma-2	2	2	1	2.2	Novel	HGTP_anticodon, cEGF
`ENSMUSG00000033434`	Gtpbp6	Putative GTP-binding protein 6	3	2	1	15.4	Mixed	AAA_16, AAA_23, ABC_tran, DO-GTPase2, Dynamin_N, FeoB_N, GTP-bdg_M, GTP-bdg_N, HflX_C, MMR_HSR1, MobB, PduV-EutP, Rad17, TniB, cobW
`ENSMUSG00000028218`	Cibar1	CBY1-interacting BAR domain-containing protein 1	2	2	1	140.8	Novel	DUF6447, FAM92, FapA, P4Ha_N, Syntaxin-6_N, Vps5
`ENSMUSG00000034681`	RNPS1	RNA-binding protein with serine-rich domain 1	5	2	1	83.6	Mixed	Macoilin, Nup35_RRM_2, PI3K_1B_p101, RRM_1, RRM_3, RRM_5, RRM_7, RRM_occluded
`ENSMUSG00000061751`	Kalrn	Kalirin	2	2	1	35.2	Ref	ALIX_LYPXL_bnd, CRAL_TRIO, CRAL_TRIO_2, I-set, IQ_SEC7_PH, Ig_2, Ig_3, Kdo, MC7, PEP-utilisers_N, PH, PH_10, PH_13, PH_19, PK_Tyr_Ser-Thr, Pkinase, Pkinase_fungal, RL11D, RhoGEF, SH3-RhoG_link, Spectrin, T4_Rnl2_C, V-set, fn3, ig
`ENSMUSG00000001844`	Zdhhc4	Palmitoyltransferase ZDHHC4	2	2	1	19.8	Novel	DHHC, DUF417
`ENSMUSG00000061702`	Tmem91	Transmembrane protein 91	2	2	1	19.8	Novel	AWPM-19, CD225
`ENSMUSG00000038708`	Golga4	Golgin subfamily A member 4	2	2	1	13.2	Ref	DUF3800, GRIP
`ENSMUSG00000005483`	Dnajb1	DnaJ homolog subfamily B member 1	2	2	1	6.6	Ref	DnaJ, DnaJ_C
`ENSMUSG00000033916`	Chmp2a	Charged multivesicular body protein 2a	3	2	1	490.6	Ref	FapA, Snf7, YlqD
`ENSMUSG00000054770`	KCTD18	BTB/POZ domain-containing protein KCTD18	2	2	1	2.2	Ref	BTB_2, BTB_3, KCTD18_C
`ENSMUSG00000063236`	—	UPF0488 protein C8orf33 homolog	2	2	1	41.8	Ref	CtsR_C, DUF3584, DUF4615, PDE4_UCR, PolC_DP2
`ENSMUSG00000117789`	Brms1	Breast cancer metastasis-suppressor 1 homolog	2	2	1	2.2	Novel	CDC45, DASH_Spc19, Dynactin_p62, EBP50_C, Glyco_transf_49, GspL_C, PIP5K, Sds3, Vfa1, Zip
`ENSMUSG00000004069`	Dnaja3	DnaJ homolog subfamily A member 3, mitochondrial	2	2	1	22.0	Ref	Anti-TRAP, DnaJ, DnaJ_C, DnaJ_CXXCXGXG, Endonuclease_7, FtrD-like
`ENSMUSG00000022043`	Trim35	E3 ubiquitin-protein ligase TRIM35	2	2	1	52.8	Ref	ATG16, Baculo_IE-1, CCDC90-like, DUF2203, DUF4200, Fez1, PRY, Prok-RING_4, SPRY, zf-B_box, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_6, zf-RING_UBOX
`ENSMUSG00000024208`	Uqcc2	Ubiquinol-cytochrome c reductase complex assembly factor 2	2	2	1	308.0	Ref	Complex1_LYR, Globin, UQCC2_CBP6
`ENSMUSG00000027706`	Sec62	Translocation protein SEC62	2	2	1	17.6	Novel	BUD22, CIS_TMP, DEP, Dsh_C, EOS1, RR_TM4-6, SLC12, Sec62, YhhN
`ENSMUSG00000039357`	Fut11	GDP-fucose protein O-fucosyltransferase 4	2	2	1	0.0	Ref	Glyco_tran_10_N, Glyco_transf_10
`ENSMUSG00000026095`	Asnsd1	Asparagine synthetase domain-containing protein 1	2	2	1	26.4	Ref	Asn_synthase, GATase_6, GATase_7, NAD_synthase, Prefoldin_2
`ENSMUSG00000073411`	H2-D1	H-2 class I histocompatibility antigen, D-B alpha chain	3	2	1	39.6	Mixed	C1-set, C2-set_2, Ig_2, Ig_3, MHC_I, MHC_I_C, Omp85, ig
`ENSMUSG00000003421`	Nosip	Nitric oxide synthase-interacting protein	2	2	1	50.6	Ref	PWI, Rtf2, U-box, zf-NOSIP, zf-Nse
`ENSMUSG00000034361`	Cpne2	Copine-2	2	2	1	6.6	Ref	C2, Copine, VMAP-M1, vWA-TerF-like
`ENSMUSG00000041420`	Meis3	Homeobox protein Meis3	3	2	1	44.0	Ref	Homeobox_KN, Homeodomain, Meis_PKNOX_N
`ENSMUSG00000000861`	Bcl11a	BCL11 transcription factor A	2	2	1	41.8	Ref	Astro_capsid_p, BSP_II, C1_4, DDHD, DNA_pol_phi, DUF2256, DUF3268, DUF4820, FAM32A, FYVE, Importin_rep_6, NOA36, Nop14, PPP4R2, RNA_pol_3_Rpc31, Ribosomal_60s, TUTase, zf-C2H2, zf-C2H2_11, zf-C2H2_2, zf-C2H2_4, zf-H2C2_2, zf-H2C2_5, zf-HIT, zf_UBZ
`ENSMUSG00000001999`	Blvra	Biliverdin reductase A	2	2	1	90.2	Novel	Biliv-reduc_cat, GFO_IDH_MocA, HYPK_UBA, NAD_binding_3, SAM_KSR1
`ENSMUSG00000031539`	Ap3m2	AP-3 complex subunit mu-2	2	2	1	13.2	Ref	Adap_comp_sub, Clat_adaptor_s, DUF11
`ENSMUSG00000026027`	Stradb	STE20-related kinase adapter protein beta	2	2	1	30.8	Ref	DUF1824, ISET-FN3_linker, Kinase-like, PK_Tyr_Ser-Thr, Pkinase
`ENSMUSG00000025156`	GPS1	COP9 signalosome complex subunit 1	4	2	1	116.6	Ref	Antitox_RHH, CTD10, GARP, PCI, RPN7, TPR_1, TPR_11, TPR_2
`ENSMUSG00000029575`	Mmab	Corrinoid adenosyltransferase MMAB	2	2	1	13.2	Ref	Cob_adeno_trans, DUF4389
`ENSMUSG00000060036`	Rpl3	Large ribosomal subunit protein uL3	3	2	1	2,756.8	Mixed	DUF6709, Ribosomal_L3
`ENSMUSG00000005873`	Reep5	Receptor expression-enhancing protein 5	2	2	1	187.0	Novel	Phage_holin_3_6, TB2_DP1_HVA22
`ENSMUSG00000036371`	Serbp1	SERPINE1 mRNA-binding protein 1	4	2	1	132.0	Ref	HABP4_PAI-RBP1, IHABP4_N
`ENSMUSG00000043445`	Pgp	Glycerol-3-phosphate phosphatase	2	2	1	39.6	Ref	Asp_Glu_race, HAD_2, Hydrolase, Hydrolase_6, Hydrolase_like, PGP_phosphatase
`ENSMUSG00000017167`	Cntnap1	Contactin-associated protein 1	2	2	1	17.6	Novel	EGF, F5_F8_type_C, Laminin_G_1, Laminin_G_2, Laminin_G_3, PRIMA1, hEGF
`ENSMUSG00000047084`	Ngrn	Neugrin	2	2	1	101.2	Ref	Dermcidin, MRP-L20, Neugrin, Ribosomal_L29, UPF0122
`ENSMUSG00000024926`	Kat5	Histone acetyltransferase KAT5	3	2	1	44.0	Mixed	Acetyltransf_1, Acetyltransf_7, MOZ_SAS, zf-MYST
`ENSMUSG00000025867`	CPLX2	Complexin-2	2	2	1	576.5	Novel	AP-5_subunit_s1, Borrelia_P83, DDHD, DUF2838, DUF4407, MDM10, RNA_polI_A34, RR_TM4-6, Spt5_N, Synaphin, VIT1
`ENSMUSG00000037936`	Scarb1	Scavenger receptor class B member 1	2	2	1	24.2	Ref	CD36, PorA, TMEM51
`ENSMUSG00000024012`	Mtch1	Mitochondrial carrier homolog 1	6	2	1	118.8	Ref	Mito_carr, YdjM
`ENSMUSG00000032112`	Trappc4	Trafficking protein particle complex subunit 4	2	2	1	231.0	Ref	PDZ_6, Sedlin_N, Sybindin
`ENSMUSG00000028484`	Psip1	PC4 and SFRS1-interacting protein	3	2	1	99.0	Ref	PWWP, SLT_3
`ENSMUSG00000032309`	Fbxo22	F-box only protein 22	2	2	1	44.0	Ref	F-box, F-box-like, FIST, FIST_C
`ENSMUSG00000060126`	Tpt1	Translationally-controlled tumor protein	3	2	1	165.0	Novel	DUF6613, TCTP
`ENSMUSG00000021619`	Atg10	Ubiquitin-like-conjugating enzyme ATG10	3	2	1	37.4	Mixed	Autophagy_act_C, zinc_ribbon_4
`ENSMUSG00000025035`	Arl3	ADP-ribosylation factor-like protein 3	2	2	1	409.2	Ref	AAA, AAA_14, AAA_18, Arf, CdhD, DUF5975, FeoB_N, G-alpha, GTP_EFTU, Gtr1_RagA, MMR_HSR1, MeaB, NACHT, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB, cobW
`ENSMUSG00000029199`	Lias	Lipoyl synthase, mitochondrial	2	2	1	41.8	Ref	Glyco_transf_4, LIAS_N, ROS_MUCR, Radical_SAM, SVWC
`ENSMUSG00000028759`	Hp1bp3	Heterochromatin protein 1-binding protein 3	2	2	1	13.2	Ref	CCD97-like_C, EMC7_beta-sandw, Linker_histone, Nop14, RRN3, SDA1, SURF2, YL1
`ENSMUSG00000032397`	Tipin	TIMELESS-interacting protein	3	2	1	48.4	Novel	Swi3
`ENSMUSG00000049339`	Retreg2	Reticulophagy regulator 2	2	2	1	46.2	Ref	INSIG, MASE2, Reticulon
`ENSMUSG00000021087`	Rtn1	Reticulon-1	2	2	1	1,835.0	Ref	DUF4736, Reticulon
`ENSMUSG00000026696`	VAMP4	Vesicle-associated membrane protein 4	2	2	1	26.4	Ref	DUF1664, DUF948, EMP24_GP25L, Fzo_mitofusin, MCU, NPV_P10, Nuf2_DHR10-like, Synaptobrevin, TFR_dimer
`ENSMUSG00000020083`	Fam241b	Protein FAM241B	3	2	1	90.2	Ref	DUF4605
`ENSMUSG00000029713`	GNB2	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	2	2	1	228.8	Ref	ANAPC4_WD40, CaM_bind, Cytochrom_D1, Ge1_WD40, NBCH_WD40, WD40, WD40_like
`ENSMUSG00000044221`	Grsf1	G-rich sequence factor 1	2	2	1	35.2	Novel	RRM_1, zf-C2H2_assoc
`ENSMUSG00000091898`	Tnnc1	Troponin C, slow skeletal and cardiac muscles	2	2	1	110.0	Novel	DUF1456, DUF1707, DUF5580, DUF6694, EF-hand_1, EF-hand_10, EF-hand_4, EF-hand_5, EF-hand_6, EF-hand_7, EF-hand_8, EF-hand_9, EMG1, Peptidase_C39_2, RNA_pol_Rpb4, SPARC_Ca_bdg, Syntaxin, TerB, UPF0154
`ENSMUSG00000020308`	Tpgs1	Tubulin polyglutamylase complex subunit 1	2	2	1	233.2	Novel	DUF2267, Dpy-30, RIIa
`ENSMUSG00000063931`	Pepd	Xaa-Pro dipeptidase	2	2	1	118.8	Novel	AMP_N, DUF3793, Peptidase_M24
`ENSMUSG00000060743`	His3.3A	Histone H3.3A	2	2	1	136.4	Ref	CBFD_NFYB_HMF, CENP-S, CENP-T_C, GHL13, Histone, PAF
`ENSMUSG00000022961`	Son	Protein SON	2	2	1	41.8	Ref	DND1_DSRM, G-patch, G-patch_2, RSRP
`ENSMUSG00000045007`	Tubg2	Tubulin gamma-2 chain	2	2	1	125.4	Novel	Misat_Tub_SegII, Sp-DndD, Tubulin, Tubulin_2, Tubulin_C
`ENSMUSG00000027215`	Cd82	CD82 antigen	2	2	1	37.4	Ref	DUF1310, DUF2070, DUF2157, DUF2981, DUF6057, OAD_gamma, Tetraspanin, Tmemb_55A
`ENSMUSG00000031897`	Psmb10	Proteasome subunit beta type-10	3	2	1	191.4	Novel	NAD_Gly3P_dh_C, Pr_beta_C, Proteasome
`ENSMUSG00000024436`	Mrps18b	Small ribosomal subunit protein mS40	2	2	1	105.6	Ref	FAM165, HTH_21, Ribosomal_S18, zf-RING_10
`ENSMUSG00000026825`	DNM1	Dynamin-1	2	2	1	48.4	Ref	AAA_28, Dynamin_M, Dynamin_N, GED, MMR_HSR1, PH, PH_11
`ENSMUSG00000068039`	Tcp1	T-complex protein 1 subunit alpha	3	2	1	70.4	Ref	Cpn60_TCP1
`ENSMUSG00000032468`	Armc8	Armadillo repeat-containing protein 8	2	2	1	11.0	Ref	Arm, Arm_2, DCB, DUF4704, Drf_GBD, ELMO_ARM, HEAT, HEAT_2, HEAT_EZ, MMS19_C, NopRA1, ParcG, RICTOR_V, RTTN_N, RasGEF_N_2, UNC45-central, Uso1_p115_head, V-ATPase_H_C
`ENSMUSG00000022837`	Iqcb1	IQ calmodulin-binding motif-containing protein 1	3	2	1	6.6	Novel	Bac_export_1, Condensin2nSMC, DUF1765, IQ
`ENSMUSG00000032251`	Irak1bp1	Interleukin-1 receptor-associated kinase 1-binding protein 1	2	2	1	39.6	Ref	MerB, SIMPL, fvmX5
`ENSMUSG00000031996`	Aplp2	Amyloid beta precursor like protein 2	2	2	1	158.4	Novel	APP_Cu_bd, APP_E2, APP_N, Activator-TraM, CDC24, CDC45, DabA, Kunitz_BPTI, VHL_C
`ENSMUSG00000008822`	Acyp1	Acylphosphatase-1	2	2	1	110.0	Ref	Acylphosphatase, DUF2871
`ENSMUSG00000058267`	Mrps14	Small ribosomal subunit protein uS14m	2	2	1	176.0	Ref	PSS, Ribosomal_S14
`ENSMUSG00000027406`	Idh3B	Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial	4	2	1	622.7	Novel	AAA_3, Iso_dh
`ENSMUSG00000024234`	Mtpap	Poly(A) RNA polymerase, mitochondrial	2	2	1	17.6	Ref	Nrap_D2, PAP_assoc, RL, TUTase, tRNA_NucTransf2
`ENSMUSG00000026418`	Tnni1	Troponin I, slow skeletal muscle	2	2	1	2.2	Novel	Apis_Csd, Atrophin-1, Auts2, BES1_N, DUF2951, DUF3915, FAM27, PDCD7, Troponin, XkdW
`ENSMUSG00000069520`	Tmem19	Transmembrane protein 19	2	2	1	0.0	Ref	DUF92, PRIMA1
`ENSMUSG00000094936`	Rbm4b	RNA-binding protein 4B	2	2	1	19.8	Ref	Calcipressin, CompInhib_SCIN, DbpA, Nup35_RRM_2, RNA_bind, RRM_1, RRM_5, RRM_7, RRM_occluded, RSD-2, zf-CCHC, zf-CCHC_2, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000030922`	Lyrm1	LYR motif-containing protein 1	2	2	1	15.4	Ref	Complex1_LYR, Complex1_LYR_2, DUF948, SKA2
`ENSMUSG00000031060`	Rbm10	RNA-binding protein 10	2	2	1	4.4	Ref	DUF4407, G-patch, G-patch_2, GSIII_N, OCRE, PRE_C2HC, RRM_1, TackOD1, zf-C2H2_4, zf-RanBP
`ENSMUSG00000029712`	ACTL6B	Actin-like protein 6B	4	2	1	129.8	Novel	Actin, MobA_MobL, MreB_Mbl
`ENSMUSG00000027534`	Snx16	Sorting nexin-16	2	2	1	15.4	Ref	DUF641, HIP1_clath_bdg, LRRFIP, PV-1, PX
`ENSMUSG00000034484`	Snx2	Sorting nexin-2	2	2	1	33.0	Novel	4HB_MCP_1, Allexi_40kDa, Arfaptin, BAR_3_WASP_bdg, BLOC1_2, DUF1870, DUF3775, DUF4618, DUF4795, Dynamin_N, Exonuc_VII_L, FAM92, FapA, Fez1, Fib_alpha, FliD_N, GrpE, HMMR_N, HR1, PX, Prefoldin_2, Sec34, Sorting_nexin, Vps5
`ENSMUSG00000029363`	Rfc5	Replication factor C subunit 5	2	2	1	13.2	Ref	AAA, AAA_11, AAA_14, AAA_16, AAA_19, AAA_22, AAA_24, AAA_25, AAA_3, AAA_30, AAA_5, AAA_7, AAA_assoc_2, ATPase_2, DEAD, DNA_pol3_delta, DNA_pol3_delta2, DUF815, Mg_chelatase, PhoH, RNA_helicase, Rad17, Rep_fac_C, ResIII, RuvB_N, Sigma54_activat, T2SSE, TIP49, Thioredoxin_6, TniB, bpMoxR
`ENSMUSG00000021236`	Entpd5	Ectonucleoside triphosphate diphosphohydrolase 5	2	2	1	4.4	Ref	GDA1_CD39, zf-rbx1
`ENSMUSG00000024474`	Ik	Protein Red	2	2	1	96.8	Ref	ArgoN, DUF1509, Lsm_interact, MCR_C, PRP38_assoc, PTPRCAP, Presenilin, RED_C, RED_N, ShlB, VIR_N
`ENSMUSG00000024560`	Cxxc1	CXXC-type zinc finger protein 1	2	2	1	2.2	Novel	Auto_anti-p27, CpG_bind_C, DUF2116, DZR, PHD, SUIM_assoc, zf-CXXC
`ENSMUSG00000028991`	Mtor	Serine/threonine-protein kinase mTOR	2	2	1	39.6	Ref	ANAPC3, Adaptin_N, DUF3385, EPSP_synthase, FAT, FATC, FRB_dom, HEAT, HEAT_2, HEAT_EZ, Nipped-B_C, PI3_PI4_kinase, TAN, TPR_19, UME, V-ATPase_H_N
`ENSMUSG00000022151`	Ttc33	Tetratricopeptide repeat protein 33	3	2	1	85.8	Ref	ATG14, ChAPs, TPR_1, TPR_11, TPR_14, TPR_16, TPR_17, TPR_19, TPR_2, TPR_9
`ENSMUSG00000031367`	AP1S2	AP-1 complex subunit sigma-2	3	2	1	17.6	Ref	Clat_adaptor_s, PulA_N1
`ENSMUSG00000041353`	FAM156A	Protein FAM156A/FAM156B	2	2	1	22.0	Ref	FAM104, PGC7_Stella
`ENSMUSG00000019188`	Hm13	Signal peptide peptidase	3	2	1	88.0	Ref	DUF4345, Peptidase_A22B, Presenilin, SPP
`ENSMUSG00000002395`	Use1	Vesicle transport protein USE1	4	2	1	228.8	Novel	AAR2, Adhes-Ig_like, Amnionless, CCB2_CCB4, Collectrin, DUF5383, MCU, MRP-L28, Orf78, Use1
`ENSMUSG00000014232`	Cluap1	Clusterin-associated protein 1	2	2	1	44.0	Novel	ATG14, BUD22, CENP-B_dimeris, Cluap1, Jnk-SapK_ap_N, PBP1_TM, RasGAP_C, Tweety
`ENSMUSG00000079317`	TRAPPC2	Trafficking protein particle complex subunit 2	2	2	1	134.2	Ref	DUF4984, Sedlin_N, Sybindin
`ENSMUSG00000038876`	Echdc1	Ethylmalonyl-CoA decarboxylase	3	2	1	72.6	Ref	ECH_1, ECH_2, Prok-RING_4, Sp-DndD, WWE, zf-C3HC4, zf-C3HC4_2, zf-C3HC4_3, zf-C3HC4_4, zf-RING_10, zf-RING_2, zf-RING_5, zf-RING_UBOX
`ENSMUSG00000045128`	Rpl18a	Large ribosomal subunit protein eL20	4	2	1	30.8	Ref	Ribosomal_L18A
`ENSMUSG00000059436`	Max	Protein max	2	2	1	48.4	Ref	ATG16, Csm1_N, DUF4140, DUF4763, DUF5595, DUF5716, DivIC, FapA, Filament, HALZ, HLH, JAKMIP_CC3, Jnk-SapK_ap_N, SLX9, SlyX, V_ATPase_I, ZapB, bZIP_2
`ENSMUSG00000024949`	Sf1	Splicing factor 1	3	2	1	35.2	Ref	KH_1, KH_5, SF1-HH, zf-CCHC, zf-CCHC_3, zf-CCHC_5
`ENSMUSG00000026203`	Dnajb2	DnaJ homolog subfamily B member 2	2	2	1	33.0	Ref	DnaJ, RPT, UIM
`ENSMUSG00000026019`	Wdr12	Ribosome biogenesis protein WDR12	2	2	1	0.0	Ref	ANAPC4_WD40, DUF6739, NBCH_WD40, NLE, NRDE-2, NUDIX_4, Pap_E4, Pneumo_att_G, RR_TM4-6, Serinc, WD40, Zip
`ENSMUSG00000021147`	Wdr37	WD repeat-containing protein 37	2	2	1	4.4	Ref	ANAPC4_WD40, CCDC90-like, DivIVA, FAM76, HALZ, IZUMO, NBCH_WD40, Nup160, WD40, YabA
`ENSMUSG00000019785`	Clvs2	Clavesin-2	2	2	1	46.2	Ref	CRAL_TRIO, CRAL_TRIO_2, CRAL_TRIO_N, PriCT_1
`ENSMUSG00000039128`	Cdc123	Translation initiation factor eIF2 assembly protein	4	2	1	19.8	Mixed	D123, Reg_prop
`ENSMUSG00000028064`	Sema4a	Semaphorin-4A	2	2	1	11.0	Ref	Ig_2, PSI, Sema
`ENSMUSG00000002949`	Timm44	Mitochondrial import inner membrane translocase subunit TIM44	2	2	1	50.6	Ref	AIM3, DUF3347, DUF4211, DUF948, FUT8_N_cat, Kinase-PolyVal, Prominin, RecG_N, Syntaxin, Syntaxin_2, TMPIT, Tim44
`ENSMUSG00000031342`	Gpm6b	Neuronal membrane glycoprotein M6-b	3	2	1	24.2	Ref	DUF373, LapA_dom, Myelin_PLP, PspB
`ENSMUSG00000022336`	EIF3E	Eukaryotic translation initiation factor 3 subunit E	2	2	1	242.0	Novel	CSN8_PSD8_EIF3K, HD_assoc, PARP_reg, PCI, SPESP1, VMAP-M1, eIF3_N
`ENSMUSG00000027180`	Fbxo3	F-box only protein 3	2	2	1	4.4	Ref	CDC45, CobT, DNA_pol_phi, DUF525, F-box, F-box-like, Nop53, RPA43_OB, SMI1_KNR4, SUKH_5, SUKH_6, Sigma70_ner
`ENSMUSG00000052105`	Mtcl1	Microtubule cross-linking factor 1	2	2	1	6.6	Ref	DUF4407, DUF4482, RSS_P20, SOGA, TGF_beta_GS
`ENSMUSG00000062006`	Rpl34	Large ribosomal subunit protein eL34	3	2	1	79.2	Ref	Ribosomal_L34e
`ENSMUSG00000026163`	Sphkap	A-kinase anchor protein SPHKAP	2	2	1	61.6	Ref	AKAP_110, DUF1133, FAM212, MAGP
`ENSMUSG00000031862`	Atp13a1	Endoplasmic reticulum transmembrane helix translocase	2	2	1	15.4	Ref	Cation_ATPase, DHHC, E1-E2_ATPase, Hydrolase, Hydrolase_3
`ENSMUSG00000074874`	Ctla2a	Protein CTLA-2-alpha	2	2	1	6.6	Ref	GST_N_5, Inhibitor_I29, Mem_trans
`ENSMUSG00000025245`	Lztfl1	Leucine zipper transcription factor-like protein 1	2	2	1	13.2	Ref	DNA_pol3_a_NI, FUSC, Hom_end_hint, Leu_zip, MAD, Methyltransf_14, Mit_ribos_Mrp51, SesA
`ENSMUSG00000047261`	Gap43	Neuromodulin	3	2	1	486.2	Mixed	HEPN_AbiU2
`ENSMUSG00000045576`	St7l	Suppressor of tumorigenicity 7 protein-like	2	2	1	8.8	Ref	CATRA-C, Clathrin, ST7, TPR_1, TPR_14, TPR_19, TPR_2, TPR_6
`ENSMUSG00000003531`	Dgcr6	Protein DGCR6	3	2	1	167.2	Ref	DGCR6, DUF2458
`ENSMUSG00000024587`	Nars1	Asparagine--tRNA ligase, cytoplasmic	3	2	1	154.0	Ref	Cir_N, tRNA-synt_2, tRNA-synt_2d, tRNA_anti-codon
`ENSMUSG00000079179`	—	—	2	2	2	24.2	Ref	DUF1091, TFIIA
`ENSMUSG00000036372`	TMEM258	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258	2	2	2	116.6	Ref	DUF6512, Ost5, Phage_holin_3_3, RIIa, Xre_MbcA_ParS_C
`ENSMUSG00000031388`	Naa10	N-alpha-acetyltransferase 10	3	2	4	187.0	Ref	Acetyltransf_1, Acetyltransf_10, Acetyltransf_13, Acetyltransf_15, Acetyltransf_3, Acetyltransf_7, Acetyltransf_9, FR47
`ENSMUSG00000074899`	SPTBN5	Spectrin beta chain, non-erythrocytic 5	3	2	4	2.2	Novel	AHSP, Spectrin
`ENSMUSG00000035762`	Tmem161b	Transmembrane protein 161B	2	2	4	2.2	Ref	Adeno_E3_14_5, DUF443, Tmemb_161AB, V-SNARE
`ENSMUSG00000055430`	Nap1l5	Nucleosome assembly protein 1-like 5	2	2	4	393.8	Novel	ANAPC15, Ax_dynein_light, Dicty_REP, HrpB7, Mst1_SARAH, NAP, OmpH, Phage_Mu_Gam, Voldacs
`ENSMUSG00000041889`	Shisa4	Protein shisa-4	3	2	4	189.2	Mixed	Shisa
`ENSMUSG00000022536`	Glyr1	Cytokine-like nuclear factor N-PAC	2	2	4	8.8	Ref	2-Hacid_dh_C, 3HCDH_N, AT_hook, ApbA, CholecysA-Rec_N, F420_oxidored, NAD_Gly3P_dh_N, NAD_binding_11, NAD_binding_2, PWWP, Sacchrp_dh_NADP, Shikimate_DH
`ENSMUSG00000028461`	Ccdc107	Coiled-coil domain-containing protein 107	2	2	4	125.4	Ref	Baculo_PEP_C, DUF1043, DUF3014, DUF3638, DUF948, GAS, Mce4_CUP1, RIC3, RskA, YihI
`ENSMUSG00000034194`	R3hcc1	R3H and coiled-coil domain-containing protein 1	2	2	4	39.6	Ref	BUD22, CSRNP_N, DUF4407, Dicty_REP, NCBP3, R3H, SDA1
`ENSMUSG00000039771`	POLR2J	DNA-directed RNA polymerase II subunit RPB11	2	2	4	147.4	Ref	RNA_pol_L, RNA_pol_L_2, SHNi-TPR
`ENSMUSG00000031585`	Gtf2e2	General transcription factor IIE subunit 2	2	2	4	30.8	Ref	PhoQ_Sensor, TFA2_Winged_2, TFIIE_beta
`ENSMUSG00000002329`	Mdp1	Magnesium-dependent phosphatase 1	2	2	4	83.6	Ref	ABM, Acid_PPase, HAD_2, Hydrolase, NIF
`ENSMUSG00000020832`	Eral1	GTPase Era, mitochondrial	2	2	4	26.4	Ref	AAA_16, AAA_28, AIG1, Arf, Dynamin_N, FeoB_N, G-alpha, GTP_EFTU, KH_1, KH_2, MMR_HSR1, PduV-EutP, Ras, Roc, RsgA_GTPase, SRPRB, TniB, cobW
`ENSMUSG00000020738`	SUMO2	Small ubiquitin-related modifier 2	2	2	4	1,544.5	Ref	DUF5010_C, Rad60-SLD, ubiquitin
`ENSMUSG00000021606`	Ndufs6	NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial	2	2	4	184.8	Ref	Chitin_synth_2, Thiolase_C, zf-CHCC
`ENSMUSG00000022556`	Hsf1	Heat shock factor protein 1	2	2	4	28.6	Ref	HMMR_C, HSF_DNA-bind, NPV_P10, Vert_HS_TF, ZapB
`ENSMUSG00000026857`	Ntmt1	N-terminal Xaa-Pro-Lys N-methyltransferase 1	2	2	4	107.8	Ref	Bin3, CTD4, Methyltransf_11, Methyltransf_12, Methyltransf_2, Methyltransf_23, Methyltransf_25, Methyltransf_29, Methyltransf_31, Methyltransf_PK, Ubie_methyltran
`ENSMUSG00000036854`	Hspb6	Heat shock protein beta-6	2	2	4	11.0	Novel	ArsA_HSP20, Crystallin, HSP20, SelB-wing_2
`ENSMUSG00000021039`	Snw1	SNW domain-containing protein 1	2	2	4	50.6	Novel	SIR4_SID, SKIP_SNW
`ENSMUSG00000024792`	Zfpl1	Zinc finger protein-like 1	2	2	4	88.0	Ref	PHD, PHD_4, zf-4CXXC_R1, zf-B_box, zf-RING_16, zf-RING_2
`ENSMUSG00000033720`	Sfxn5	Sideroflexin-5	2	2	4	30.8	Ref	DUF1980, SFXNs
`ENSMUSG00000025024`	Smndc1	Survival of motor neuron-related-splicing factor 30	2	2	4	59.4	Ref	ATP_sub_h, SMN, TUDOR
`ENSMUSG00000025735`	Rhbdl1	Rhomboid-related protein 1	2	2	4	44.0	Novel	DUF5580, HPP, Rhomboid
`ENSMUSG00000004892`	Bcan	Brevican core protein	3	2	4	68.2	Mixed	I-set, Ig_2, Ig_3, Izumo-Ig, Lectin_C, V-set, Xlink, ig
`ENSMUSG00000028634`	—	—	2	2	4	79.2	Novel	Activin_recp, SIP1
`ENSMUSG00000040462`	Os9	Protein OS-9	2	2	4	105.6	Ref	IL4Ra_N, PRKCSH, PRKCSH_1, RRN3, SNAP-25
`ENSMUSG00000019210`	Atp6v1e1	V-type proton ATPase subunit E 1	2	2	4	1,973.6	Novel	ATP-synt_B, DUF4288, Folliculin, NifW, O-antigen_lig, Par3_HAL_N_term, vATP-synt_E
`ENSMUSG00000044573`	Acp1	Low molecular weight phosphotyrosine protein phosphatase	2	2	4	24.2	Ref	LMWPc, MmeI_Mtase, TMEM206
`ENSMUSG00000032966`	Fkbp1a	Peptidyl-prolyl cis-trans isomerase FKBP1A	2	2	4	1,683.1	Ref	FKBP_C, Glyoxalase_4
`ENSMUSG00000027316`	Gfra4	GDNF family receptor alpha-4	3	2	4	61.6	Novel	GDNF
`ENSMUSG00000072772`	Grcc10	Protein C10	2	2	4	668.9	Ref	FAST_2, HicA_toxin, P_C10
`ENSMUSG00000024146`	Cript	Cysteine-rich PDZ-binding protein	2	2	4	272.8	Ref	Cas_CXXC_CXXC, Cript, DUF1272, DUF2039, DZR_2, Fin, Gin, PLATZ, Rhodanese_C, zf-RING_9, zinc_ribbon_2
`ENSMUSG00000129504`	Nefm	Neurofilament medium polypeptide	2	2	4	6.6	Ref	APG6_N, ATG14, ATG16, BCAS2, Baculo_PEP_C, CALCOCO1, CCDC22, CENP-F_leu_zip, CEP63, Crescentin, DHquinase_II, DUF1664, DUF3450, DUF4200, DUF6468, ERM_helical, Fez1, Fib_alpha, Filament, Filament_head, GAS, Golgin_A5, MAD, NPV_P10, Nuf2_DHR10-like, Prefoldin, SERRATE_Ars2_N, Spc29, TMF_DNA_bd, TolA_bind_tri, Tup_N
`ENSMUSG00000039536`	Stau1	Double-stranded RNA-binding protein Staufen homolog 1	3	2	4	37.4	Ref	DND1_DSRM, Staufen_C, dsrm
`ENSMUSG00000030495`	Slc7a10	Asc-type amino acid transporter 1	2	2	4	30.8	Ref	AA_permease, AA_permease_2, DUF5752, Trypan_PARP
`ENSMUSG00000074247`	Dda1	DET1- and DDB1-associated protein 1	2	2	4	41.8	Ref	DDA1, DUF3138, Lgl_C
`ENSMUSG00000027593`	Raly	RNA-binding protein Raly	3	2	4	116.6	Ref	RRM_1, RRM_5
`ENSMUSG00000052560`	Cpne8	Copine-8	2	2	4	17.6	Ref	C2, Copine, FG-GAP_3, Glyco_hydro_106, vWA-TerF-like
`ENSMUSG00000024962`	Vegfb	Vascular endothelial growth factor B	2	2	4	147.4	Ref	CXCXC, PDGF, VEGF_C
`ENSMUSG00000032383`	Ppib	Peptidyl-prolyl cis-trans isomerase B	2	2	4	517.0	Novel	DUF4134, Pro_isomerase
`ENSMUSG00000062753`	Smim29	Small integral membrane protein 29	2	2	4	41.8	Ref	ABC2_membrane_3, BCCT, COX14, CcmH, DUF2371, DUF3267, DUF3619, DUF4245, Hol_Tox, PaREP1
`ENSMUSG00000053553`	Chlsn	Cholesin	2	2	4	15.4	Ref	AC_N, NOT2_3_5, WKF
`ENSMUSG00000028066`	Pmf1	Polyamine-modulated factor 1	2	2	4	11.0	Ref	BLOC1_2, BRI3BP, DUF16, DUF1664, DUF4618, Ku_PK_bind, MnmE_helical, Mod_r, Nnf1, OmpH, QueF, RmuC, SYCP2_ARLD, SlyX, TSC22, TetR_C_4, Uso1_p115_C, Wtap, betaPIX_CC
`ENSMUSG00000038244`	Mical2	[F-actin]-monooxygenase MICAL2	2	2	4	59.4	Ref	DUF6780, LIM, TNFR_c6, bMERB_dom, zf-C3HC4_2, zf-RRN7
`ENSMUSG00000079426`	ARPC4	Actin-related protein 2/3 complex subunit 4	2	2	4	211.2	Ref	ARPC4, Cmr7A, GPS2_interact, Sm_like
`ENSMUSG00000055762`	Eef1d	Elongation factor 1-delta	7	2	4	41.8	Mixed	CCD48, EF-1_beta_acid, EF1_GNE, JIP_LZII, KASH_CCD, Lzipper-MIP1, PRKG1_interact, PilJ, RF-1, Striatin, TSC22, TolA_bind_tri, Trimer_CC
`ENSMUSG00000057147`	Dph6	Diphthine--ammonia ligase	2	2	4	4.4	Ref	Diphthami_syn_2, tRNA_Me_trans
`ENSMUSG00000031728`	Znf821	Zinc finger protein 821	2	2	4	13.2	Ref	CDC45, CPSF73-100_C, DUF5679, DUF5810, DUF6011, DZR, HMBD, Rad50_zn_hook, zf-C2H2, zf-C2H2_11, zf-C2H2_4, zf-C2H2_6, zf-C2H2_9, zf-C2H2_jaz, zf-Di19, zf-H2C2_2, zf-HYPF, zf-met
`ENSMUSG00000032359`	Ctsh	Pro-cathepsin H	2	2	4	33.0	Ref	Inhibitor_I29, Peptidase_C1, Peptidase_C1_2
`ENSMUSG00000024610`	Cd74	H-2 class II histocompatibility antigen gamma chain	2	2	4	17.6	Ref	DUF2722, DUF6082, MHC2-interact, MHCassoc_trimer, Thyroglobulin_1

DV Enrichment by Expression Level (CPM from upstream transcriptomics)

Baseline DV Rate

8.2%

673 / 8,241 genes

Peak DV Rate

14.1%

High (50-200) bin

Peak Enrichment

1.7×

vs baseline

Chi-square trend

p < 0.001

Expression Bin	Total Genes	DV Genes	DV %	Fold vs Baseline
Silent (<1) CPM	558	10	1.8%	0.2×
Low (1-10) CPM	2,664	95	3.6%	0.4×
Moderate (10-50) CPM	2,820	269	9.5%	1.2×
High (50-200) CPM	1,455	205	14.1%	1.7×
Very high (>=200) CPM	744	94	12.6%	1.5×

Bar height shows the percentage of genes that are domain-variant within each expression bin. Dashed line = overall DV rate (8.2%). If DV enrichment increases monotonically with expression, ascertainment bias (more reads = more isoform discovery) is the dominant driver. Enrichment above baseline at specific expression tiers may indicate biological enrichment beyond the detection effect. CPM from 454,505 mRNA reads (0.1% rRNA excluded).

ORF Type Distribution

NMD Prediction by ORF Type

NMD Candidates by Evidence Tier

NMD Expression Validation (CPM from upstream transcriptomics)

NMD Expression Rate

97.2%

281 / 289

Non-NMD Expression Rate

95.2%

10,239 / 10,753

Fold Difference

1.0×

non-NMD / NMD

Tier 1 NMD Expression

95.6%

108 / 113

NMD Candidates (n=289)

Non-NMD (n=10,753)

Box plots show median (line), IQR (box), and 1.5×IQR whiskers. Individual transcript CPM values are overlaid as jittered points. NMD candidates are expressed at 97.2% (median 6.6 CPM) vs 95.2% (median 11.0 CPM) for non-NMD — no evidence of NMD-mediated degradation.

Output Files

blastp.with_title.tsv HPC 1.7 MB gene_functional_summary.tsv HPC 331 KB isoform_annotations.tsv HPC 2.4 MB job.yml HPC 608 B pfam.domtblout HPC 23.6 MB results_summary.json HPC 1305.6 KB

Provenance

Execution	Expression quantification summary
Completed	2026-03-01T04:30:19+00:00

RO-Crate 1.1 Workflow RO-Crate 1.0 FAIR

This analysis is packaged as a Research Object Crate with machine-readable provenance and FAIR metadata.

RO-Crate Metadata (JSON-LD)

Show/hide raw JSON-LD

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            "description": "Pfam domain search, SwissProt homology, NMD prediction, and functional diversity classification for ONT transcript isoforms.",
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        {
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            "name": "Dissection and collection of tissue from 10-week-old male mice",
            "description": "Surgical dissection and collection of cortex, pituitary, and liver tissues from 10-week-old male mice, performed by Jackson Laboratory surgical team (Bar Harbor, ME, USA). Tissues snap-frozen and shipped internationally to Australia via dry shipper using FedEx.",
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                    "text": "# SOP: Dissection and Collection of Tissue from 10-Week-Old Male Mice\n\n**Applies to:** 10-week-old male mice (C57BL/6J, DBA/2J, or other inbred strains)\n**Tissues collected:** Cortex, pituitary, liver\n**Performed by:** Surgical team, The Jackson Laboratory, Bar Harbor, ME, USA\n**Shipping:** Dry shipper via FedEx International Priority to Australia\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nTo obtain high-quality, snap-frozen tissue samples (cortex, pituitary, liver) from 10-week-old male mice for downstream molecular analysis (RNA extraction, DNA extraction, proteomics). Tissues are collected at The Jackson Laboratory and shipped internationally to Australia under cryogenic conditions to preserve nucleic acid and protein integrity.\n\n## Scope\n\nAll personnel involved in the planning, coordination, dissection, tissue collection, and shipment of mouse tissue samples between The Jackson Laboratory (USA) and the receiving laboratory (Australia).\n\n## Responsibilities\n\n* **Principal Investigator (receiving lab):** Define experimental design, strain requirements, tissue list, and sample labelling scheme. Coordinate import permits and institutional approvals.\n* **Jackson Laboratory surgical team:** Perform euthanasia, dissection, tissue collection, snap-freezing, and packaging for shipment.\n* **Shipping coordinator:** Arrange FedEx International Priority shipment, customs documentation, and dry shipper logistics.\n* **Receiving lab personnel:** Receive shipment, verify sample integrity, transfer to long-term storage, and log receipt.\n\n## Regulatory and Ethical Requirements\n\n* All animal procedures must be approved by The Jackson Laboratory IACUC (Institutional Animal Care and Use Committee) under an active protocol.\n* Receiving institution must hold a valid **import permit** for biological materials from the relevant national authority (e.g., Australian Department of Agriculture, Fisheries and Forestry \u2014 DAFF).\n* Ensure compliance with CITES regulations (not typically applicable for laboratory mice, but verify for specific strains).\n* **Biosecurity:** Frozen tissues from SPF (Specific Pathogen Free) mice. Health monitoring reports from Jackson Laboratory should accompany the shipment.\n\n---\n\n## Materials and Equipment\n\n### At Jackson Laboratory (dissection)\n\n* Certified CO\u2082 euthanasia station or injectable anaesthetic (as per IACUC protocol)\n* Cervical dislocation tools (secondary method of euthanasia)\n* Sterile surgical instruments: fine scissors, forceps (curved and straight), scalpel with #10 and #15 blades, micro-dissecting forceps, bone rongeurs (for skull)\n* Sterile surgical drapes and pads\n* Sterile PBS (phosphate-buffered saline), ice-cold\n* Liquid nitrogen in Dewar flask\n* Pre-labelled cryovials (1.5\u20132.0 mL, cryogenic-rated, screw-cap)\n* Cryogenic labels (resistant to LN\u2082 and \u221280 \u00b0C)\n* RNAlater (optional, as backup for tissues not immediately snap-frozen)\n* Absorbent pads and biohazard waste bags\n* PPE: lab coat, gloves, face shield (for LN\u2082 handling)\n\n### For shipping\n\n* **Dry shipper** (vapour-phase liquid nitrogen dewar, e.g., MVE SC 4/2V or Taylor-Wharton CX100)\n* Cryogenic storage canes or racks\n* Secondary containment bags (leak-proof, absorbent material)\n* FedEx International Priority shipping account\n* Customs documentation: commercial invoice, packing list, import permit, health certificate, AQIS declaration\n* Temperature data logger (optional but recommended)\n\n### At receiving laboratory (Australia)\n\n* \u221280 \u00b0C ultra-low freezer with allocated space\n* Cryogenic gloves and face shield\n* Sample inventory system / database\n\n---\n\n## Procedure\n\n### 1. Pre-Dissection Planning\n\n1. Confirm **mouse strain, sex (male), and age (10 weeks \u00b1 2 days)** with Jackson Laboratory.\n2. Provide Jackson Laboratory with:\n   * Number of animals required (biological replicates)\n   * Tissue list: **cortex, pituitary, liver**\n   * Sample labelling scheme (e.g., strain_tissue_replicate: C57_CTX_1, DBA_PIT_3)\n   * Any special handling instructions (e.g., morning collection to control circadian effects)\n3. Confirm **IACUC protocol** is active and covers the planned procedures.\n4. Obtain **Australian import permit** (DAFF) for frozen animal tissues. Allow 4\u20136 weeks lead time.\n5. Arrange **FedEx International Priority** account and confirm dry shipper availability and return logistics.\n\n### 2. Animal Preparation\n\n1. House mice under standard conditions (12 h light/dark cycle, ad libitum food and water, 20\u201324 \u00b0C) for at least 1 week prior to collection to minimise stress-related transcriptomic changes.\n2. Record for each animal:\n   * Strain and stock number\n   * Date of birth and age at collection\n   * Body weight\n   * Cage number and housing conditions\n   * Any health observations or abnormalities\n3. Fast animals for **4\u20136 hours** prior to collection if liver metabolomic consistency is required (optional \u2014 confirm with PI).\n\n### 3. Euthanasia\n\n1. Euthanise by **CO\u2082 inhalation** (gradual displacement, 30\u201370% chamber volume/min) followed by **cervical dislocation** as secondary confirmation, in accordance with AVMA Guidelines and IACUC protocol.\n2. Confirm death: absence of heartbeat, respiration, and pedal reflex.\n3. Record **time of death** \u2014 proceed to dissection immediately (within 5 min) to minimise RNA degradation.\n\n### 4. Tissue Dissection and Collection\n\nPerform all dissections on a clean, sterile surface. Use separate sterile instruments for each tissue to prevent cross-contamination. Work quickly \u2014 total dissection time per animal should be **<15 minutes**.\n\n#### 4a. Brain \u2014 Cortex\n\n1. Decapitate the mouse. Remove the skin overlying the skull.\n2. Using bone rongeurs or fine scissors, carefully open the skull along the sagittal suture from the foramen magnum to the olfactory bulbs.\n3. Gently lift and peel back the skull plates to expose the brain.\n4. Using curved forceps, carefully lift the brain from the cranial cavity, severing cranial nerves and the spinal cord at the brainstem.\n5. Place the brain dorsal-side up on a cold surface (glass plate on ice or cold PBS-dampened filter paper).\n6. Using a scalpel or razor blade, remove the **cerebral cortex** by making a coronal cut posterior to the olfactory bulbs and peeling the cortical hemispheres away from the underlying structures (hippocampus, striatum, thalamus).\n7. Transfer cortex tissue immediately to a **pre-labelled cryovial**.\n8. **Snap-freeze** by plunging the cryovial into liquid nitrogen. Hold submerged for \u226530 seconds.\n\n#### 4b. Pituitary\n\n1. After removing the brain, examine the base of the skull (sella turcica).\n2. The **pituitary gland** is a small (~1 mm), pinkish structure sitting in the sella turcica.\n3. Using fine-tipped forceps, gently tease the pituitary free from the surrounding membrane and bone.\n4. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen immediately.\n5. **Note:** The pituitary is very small \u2014 take care not to lose or fragment it. If it fragments, collect all pieces.\n\n#### 4c. Liver\n\n1. Open the abdominal cavity with a midline ventral incision from pelvis to sternum.\n2. Retract the intestines to expose the liver.\n3. Identify the **left lateral lobe** (largest, most accessible lobe).\n4. Excise a **~50\u2013100 mg piece** (approximately 5 \u00d7 5 \u00d7 3 mm) from the left lateral lobe using a clean scalpel or razor blade.\n5. Briefly blot on sterile filter paper to remove excess blood.\n6. Transfer to a **pre-labelled cryovial** and snap-freeze in liquid nitrogen.\n7. If the entire liver is required, excise all lobes, record total liver weight, and snap-freeze in separate vials.\n\n### 5. Post-Dissection\n\n1. Transfer all snap-frozen cryovials from liquid nitrogen to a **\u221280 \u00b0C freezer** within 1 hour of collection.\n2. Dispose of carcasses and biological waste according to Jackson Laboratory institutional procedures.\n3. Clean and decontaminate all instruments and work surfaces.\n4. Complete the **dissection log** for each animal:\n\n   | Field | Record |\n   |---|---|\n   | Animal ID | Strain, stock #, ear tag/toe clip |\n   | Date and time of euthanasia | |\n   | Body weight (g) | |\n   | Tissues collected | Cortex, pituitary, liver |\n   | Tissue appearance | Normal / abnormal (describe) |\n   | Cryovial labels | List all vial IDs |\n   | Operator | Name and signature |\n   | Notes | Any deviations or observations |\n\n### 6. Packaging for International Shipment\n\n1. **Charge the dry shipper** with liquid nitrogen at least 24 hours before packing. Drain excess liquid \u2014 the dry shipper must contain only **vapour-phase nitrogen** (no free liquid) for air transport compliance (IATA).\n2. Verify the dry shipper holds temperature below **\u2212150 \u00b0C** and has a rated hold time sufficient for transit (typically 10\u201314 days).\n3. Place cryovials in **cryogenic canes or racks** and insert into the dry shipper.\n4. Seal the dry shipper and place in the **outer shipping container** (insulated box or hard case).\n5. Include **absorbent material** inside the secondary containment bag (regulatory requirement for biological specimens).\n6. Attach a **temperature data logger** (optional but recommended) to monitor transit conditions.\n7. Prepare shipping documentation:\n   * **Commercial invoice** (contents: frozen mouse tissue samples, no commercial value / research use only)\n   * **Packing list** (number of vials, tissue types, strain, animal IDs)\n   * **Australian import permit** (DAFF-issued)\n   * **Health monitoring report** from Jackson Laboratory (SPF status)\n   * **Dangerous goods declaration** \u2014 Class 9 UN3373 Biological Substance Category B (if required by carrier) or exempt if shipped as non-infectious, non-hazardous research specimens in a dry shipper\n   * **IATA shipper's declaration** for magnetized material (dry shipper contains no free liquid but is classified as a cryogenic vessel)\n\n### 7. Shipment\n\n1. Schedule **FedEx International Priority** pickup from Jackson Laboratory (Bar Harbor, ME, USA).\n2. Ship **Monday\u2013Wednesday** to avoid weekend delays at customs.\n3. Estimated transit time: **3\u20135 business days** (Bar Harbor \u2192 Australian destination).\n4. Provide the receiving lab with the **tracking number** and expected arrival date.\n5. Alert the receiving lab to arrange customs clearance and immediate collection upon arrival.\n\n### 8. Receipt at Australian Laboratory\n\n1. **Collect the shipment immediately** upon arrival \u2014 do not allow it to sit at the depot.\n2. Open the dry shipper and verify:\n   * All cryovials are present and match the packing list\n   * Vials are still frozen (frost visible on vials)\n   * No signs of thawing (condensation, liquid in vials)\n   * Temperature data logger reading (if included) \u2014 confirm temperature remained below \u2212150 \u00b0C throughout transit\n3. Transfer all cryovials immediately to a **\u221280 \u00b0C freezer**.\n4. Log receipt in the laboratory sample inventory:\n\n   | Field | Record |\n   |---|---|\n   | Date received | |\n   | Shipment tracking # | |\n   | Dry shipper condition | Frost present / temperature OK |\n   | Number of vials received | |\n   | Vials match packing list? | Yes / No (note discrepancies) |\n   | Storage location | Freezer ID, shelf, box, position |\n   | Received by | Name and signature |\n\n5. Return the **dry shipper** to Jackson Laboratory per agreed logistics (typically FedEx return shipment).\n\n---\n\n## Quality Control\n\n* **RNA integrity:** Downstream RNA extraction should target RIN/RQN \u2265 7.0 for all tissues. Pituitary may yield lower due to small size \u2014 RQN \u2265 5.0 acceptable.\n* **Dissection time:** Total time from euthanasia to snap-freeze should be **<15 minutes per animal**. Record actual times.\n* **Freeze-thaw:** Tissues must not undergo any freeze-thaw cycles before processing. Monitor freezer temperature logs.\n\n## Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Pituitary fragmented or lost | Record in log; may need to pool from multiple animals or exclude |\n| Tissue partially thawed on arrival | Record in log; process immediately for extraction; note in downstream QC |\n| Customs delay >48 h | Contact FedEx and customs broker; verify dry shipper hold time is sufficient |\n| Dry shipper running low | If hold time is marginal, arrange emergency transfer to local \u221280 \u00b0C |\n| Import permit expired | Do not ship; renew permit before proceeding (allow 4\u20136 weeks) |\n| Animal health abnormality | Record observation; PI to decide whether to include or exclude the animal |\n\n## Waste Disposal\n\n* Mouse carcasses: dispose per Jackson Laboratory IACUC-approved procedures (incineration or licensed biological waste contractor).\n* Sharps (scalpel blades, needles): sharps container.\n* Liquid nitrogen: allow to evaporate in well-ventilated area. Do not pour down drains.\n\n## Records to Maintain\n\n* Approved IACUC protocol number\n* Australian import permit (DAFF) \u2014 copy\n* Dissection logs for each animal\n* Shipping documentation (commercial invoice, packing list, tracking number)\n* Temperature logger data (if used)\n* Sample receipt log\n* Freezer storage map / inventory database entry\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-40",
            "@type": "LabProtocol",
            "name": "Simultaneous DNA and RNA extraction from animal tissues using QIAGEN AllPrep DNA/RNA Mini Kit",
            "description": "Simultaneous purification of genomic DNA and total RNA from the same tissue sample (\u226430 mg) using selective AllPrep DNA spin columns and RNeasy silica membrane technology. Includes on-column DNase treatment for RNA.",
            "url": {
                "@id": "https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/multianalyte-and-virus/allprep-dna-rna-mini-kit"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "# SOP: Simultaneous DNA and RNA Extraction from Animal Tissues using QIAGEN AllPrep DNA/RNA Mini Kit\n\n**Applies to:** Fresh or frozen animal tissues \u226430 mg per spin column\n**Kit:** QIAGEN AllPrep DNA/RNA Mini Kit (Cat. No. 80204)\n**Location:** __________\n\n---\n\n## Document Control\n\n* **SOP ID:** __________\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Review Due:** __________\n* **Author:** __________\n* **Approved By:** __________\n\n**Change History**\n\n| Version | Date       | Description     | Author     |\n| ------- | ---------- | --------------- | ---------- |\n| 1.0     | __________ | Initial release | __________ |\n\n---\n\n## Purpose\n\nSimultaneous purification of genomic DNA and total RNA from the same tissue sample using the AllPrep DNA spin column (selective DNA binding in high-salt buffer) and the RNeasy spin column (silica membrane RNA binding). This enables paired DNA/RNA analysis from a single biopsy or tissue aliquot.\n\n## Scope\n\nAll personnel performing dual DNA/RNA extraction from animal tissues in this laboratory.\n\n## Responsibilities\n\n* **Operators:** Follow this SOP, record batch details, and maintain RNase-free practice.\n* **Supervisors:** Ensure training, PPE compliance, and kit maintenance.\n\n## Safety and RNase-Free Practice\n\n* Wear lab coat, gloves, and eye protection.\n* Work in a clean area. Treat benches and tools with RNase decontamination solution.\n* \u03b2-mercaptoethanol is toxic and has a strong odor. Handle in a fume hood.\n* Use RNase-free tubes, tips, and reagents only. Change gloves frequently.\n* Buffer RLT Plus contains guanidine isothiocyanate \u2014 harmful if swallowed or in contact with skin. Do not mix with bleach.\n\n## References\n\n* QIAGEN AllPrep DNA/RNA Mini Handbook, November 2020.\n* NASA GeneLab SOP GL-SOP-3.1 v1.0.\n\n---\n\n## Materials and Equipment\n\n**Provided in kit (Cat. No. 80204, 50 preps)**\n\n* Buffer RLT Plus\n* Buffer RW1\n* Buffer RPE (concentrate \u2014 add ethanol before first use)\n* Buffer AW1\n* Buffer AW2\n* AllPrep DNA Mini Spin Columns\n* RNeasy Mini Spin Columns\n* Collection Tubes (1.5 mL and 2 mL)\n* RNase-free Water\n\n**Not supplied**\n\n* \u03b2-mercaptoethanol (\u03b2-ME)\n* 96\u2013100% ethanol\n* 70% ethanol (freshly prepared)\n* RNase-free DNase Set (Cat. No. 79254) \u2014 for on-column DNase treatment\n* RNase/DNase-free 1.5 mL tubes\n* Microcentrifuge capable of \u226512,000 \u00d7 g\n* Homogenization tools: rotor-stator homogenizer, or TissueLyser with stainless steel beads (3\u20137 mm), or RNase-free pestle, or mortar and pestle with liquid nitrogen\n* Optional: RNase-free syringe with 20-gauge needle\n\n---\n\n## Preparations (before each extraction session)\n\n1. **Prepare Buffer RPE** (first use only)\n   * Add **4 volumes of 96\u2013100% ethanol** to the concentrate. Mark the bottle to indicate ethanol has been added.\n\n2. **Prepare fresh Lysis Buffer**\n   * Add **10 \u00b5L \u03b2-mercaptoethanol per 1 mL Buffer RLT Plus**. Mix well. Prepare only what you need; discard unused buffer.\n\n3. **Prepare DNase Mix** (per sample)\n   * Dissolve lyophilized DNase I (1500 Kunitz units) in **550 \u00b5L** RNase-free water. Mix gently. Aliquot and store at \u221220 \u00b0C (stable up to 9 months; thawed aliquots stable at 2\u20138 \u00b0C for 6 weeks).\n   * Per sample: **10 \u00b5L DNase I stock + 70 \u00b5L Buffer RDD**. Mix by gentle inversion \u2014 **do not vortex**.\n\n4. **Pre-chill microcentrifuge** to 4 \u00b0C if required for lysate clarification.\n\n5. **Heat 2 mL RNase/DNase-free water** to 70 \u00b0C for DNA elution (optional, improves yield).\n\n6. **Input tissue mass**\n\n   | Tissue type | Maximum input |\n   |---|---|\n   | Standard soft tissue | \u226430 mg |\n   | Muscle, heart, skin | \u226415 mg (LN\u2082-preserved) or \u226410 mg (RNAlater) |\n   | Liver | \u226420 mg |\n\n---\n\n## Procedure\n\n### 1. Tissue Disruption and Homogenization\n\n1. Weigh tissue (\u226430 mg). Place in a pre-chilled RNase-free tube on ice.\n2. Add **600 \u00b5L Buffer RLT Plus** (with \u03b2-ME).\n3. Homogenize immediately using one of the following:\n\n   **Option A \u2014 Rotor-stator:**\n   Homogenize for **30\u201345 s** until no visible tissue pieces remain.\n\n   **Option B \u2014 TissueLyser / bead mill:**\n   Add a single **5 mm stainless steel bead**. Run at **25 Hz for 2 \u00d7 2 min** (rotate adapter halfway).\n\n   **Option C \u2014 Mortar & pestle with liquid nitrogen:**\n   Grind tissue to a fine powder under LN\u2082. Transfer powder to a tube, let LN\u2082 evaporate, then add Buffer RLT Plus and vortex.\n\n   **Option D \u2014 Needle and syringe:**\n   Pass lysate through a **20-gauge needle** at least **5\u201310 times** until homogeneous.\n\n4. Centrifuge the lysate at **full speed (\u226512,000 \u00d7 g) for 3 min**, RT.\n5. Carefully transfer the supernatant to a new tube. **Do not disturb the pellet.**\n\n### 2. Bind Genomic DNA\n\n1. Transfer the cleared lysate onto an **AllPrep DNA Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 30 s**, RT.\n3. **Set aside the DNA column** at 4 \u00b0C (or RT for up to 1 h) \u2014 proceed to RNA purification first.\n4. **Save the flow-through** for RNA isolation.\n\n### 3. Adjust RNA Binding Conditions\n\n1. Add **1 volume of freshly prepared 70% ethanol** to the flow-through (e.g. 600 \u00b5L ethanol to 600 \u00b5L flow-through). For **liver tissue**, use **50% ethanol** instead.\n2. Mix well by pipetting. **Do not centrifuge.** A precipitate may form \u2014 this is normal; proceed immediately.\n\n### 4. Bind and Wash RNA\n\n1. Transfer up to **700 \u00b5L** of the sample onto an **RNeasy Mini Spin Column** in a 2 mL collection tube.\n2. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Repeat until all sample has passed through the column.\n4. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 5. On-Column DNase Treatment\n\n1. Pipette **80 \u00b5L DNase Mix** (10 \u00b5L DNase I + 70 \u00b5L Buffer RDD) directly onto the membrane.\n2. Incubate at **RT for 15\u201330 min** (30 min recommended for maximum removal).\n3. Add **350 \u00b5L Buffer RW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n\n### 6. Wash and Dry RNA Column\n\n1. **Replace the collection tube** with a new 2 mL tube.\n2. Add **500 \u00b5L Buffer RPE** (ethanol added). Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add another **500 \u00b5L Buffer RPE**. Centrifuge at **\u22658,000 \u00d7 g for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 7. Elute RNA\n\n1. Place the RNeasy column in a new **1.5 mL collection tube**.\n2. Add **30\u201350 \u00b5L RNase-free water** directly onto the membrane.\n3. Incubate **1\u20135 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** RNase-free water for maximum yield. Pool eluates if the second elution is \u226530% of the first.\n6. Place RNA on ice immediately.\n\n### 8. Wash Genomic DNA Column\n\n1. Retrieve the AllPrep DNA column from step 2.\n2. Add **500 \u00b5L Buffer AW1**. Centrifuge at **\u22658,000 \u00d7 g for 15 s**, RT. Discard flow-through.\n3. Add **500 \u00b5L Buffer AW2**. Centrifuge at **full speed (\u226514,000 \u00d7 g) for 2 min**, RT, to dry the membrane.\n4. Optional: place column in a new 2 mL tube and centrifuge at **full speed for 1 min** to eliminate residual ethanol.\n\n### 9. Elute Genomic DNA\n\n1. Place the AllPrep DNA column in a new **1.5 mL collection tube**.\n2. Add **50\u2013100 \u00b5L RNase/DNase-free water** (pre-warmed to 70 \u00b0C for improved yield) directly onto the membrane.\n3. Incubate **2 min** at RT.\n4. Centrifuge at **\u22658,000 \u00d7 g for 1 min**, RT.\n5. Optional: repeat with a second **30 \u00b5L** for maximum yield.\n\n### 10. Quantification and Quality Assessment\n\n**RNA:**\n* Measure concentration by Qubit RNA assay or NanoDrop A260.\n* Record A260/280 (expect \u22651.8) and A260/230 (expect \u22651.5) ratios.\n* Assess integrity by Fragment Analyzer, Bioanalyzer, or TapeStation (target RQN/RIN \u22657 for most applications).\n\n**DNA:**\n* Measure concentration by Qubit dsDNA assay or NanoDrop A260.\n* Record A260/280 (expect ~1.8) and A260/230 ratios.\n* Assess fragment size by TapeStation Genomic DNA assay or agarose gel if required.\n\n### 11. Storage\n\n* **RNA:** Keep on ice for immediate use. Store at **\u221280 \u00b0C** for long term.\n* **DNA:** Store at **\u221220 \u00b0C** (short term) or **\u221280 \u00b0C** (long term). Avoid repeated freeze-thaw cycles.\n\n---\n\n## Special Considerations for Muscle, Heart, and Skin Tissues\n\nThese tissues are dense and fibrous, leading to potential column clogging and lower yields.\n\n* Start with **\u226415 mg** (LN\u2082-preserved) or **\u226410 mg** (RNAlater-preserved).\n* **Pre-warm** Buffers RW1 and RPE to **37 \u00b0C** for 30 min before use.\n* Centrifuge lysate **twice** at full speed to minimize cell debris.\n* Load a maximum of **400 \u00b5L** per column pass.\n* If the column clogs: incubate wash solution on column for **5 min at 37 \u00b0C** before centrifuging.\n* Elute with **pre-warmed (37 \u00b0C) water** in two passes (50 \u00b5L + 30 \u00b5L); combine if second elution is \u226530% of first.\n* If yields remain low: process **two 15 mg aliquots separately** and combine eluates.\n\n---\n\n## Acceptance and Rejection Criteria\n\n* Elution volume and expected yield range documented per tissue type.\n* No visible carryover of debris in eluate.\n* Proceed if spectrophotometric and integrity checks meet project-specific thresholds.\n\n## Expected Yields\n\n| Tissue type | DNA yield | RNA yield |\n|---|---|---|\n| Liver (20 mg) | 10\u201330 \u00b5g | 20\u201360 \u00b5g |\n| Brain (20 mg) | 5\u201315 \u00b5g | 5\u201320 \u00b5g |\n| Kidney (20 mg) | 5\u201315 \u00b5g | 10\u201340 \u00b5g |\n| Heart/Muscle (15 mg) | 2\u201310 \u00b5g | 1\u201310 \u00b5g |\n| Spleen (20 mg) | 15\u201340 \u00b5g | 15\u201360 \u00b5g |\n\n## Troubleshooting\n\n| Problem | Possible cause | Solution |\n|---|---|---|\n| Low RNA yield | Incomplete lysis | Increase homogenization time; ensure \u03b2-ME was added to RLT Plus |\n| Low RNA yield | Column overloaded | Reduce input tissue mass |\n| Low DNA yield | Insufficient lysis | Ensure thorough mechanical disruption before loading AllPrep DNA column |\n| Low DNA yield | DNA column dried out | Do not leave DNA column at RT for >1 h |\n| Genomic DNA in RNA | DNase treatment insufficient | Extend incubation to 30 min; verify DNase I activity |\n| RNA in DNA | Carryover of flow-through | Avoid disturbing interface when transferring lysate |\n| Column clogging | Tissue debris | Pre-clear lysate by additional centrifugation; reduce input mass |\n| Low A260/230 | Guanidine salt carryover | Add an extra RPE/AW2 wash step |\n\n## Waste Disposal\n\n* Collect \u03b2-mercaptoethanol and guanidine-containing wastes according to institutional chemical waste procedures.\n* Dispose of biological materials under local biosafety rules.\n* Spin columns with bound nucleic acids are non-hazardous after elution.\n\n## Records to Maintain\n\n* Date, operator, sample ID, tissue type and mass, homogenization method, reagent lot numbers, elution volume(s), DNA and RNA concentrations, purity ratios, integrity assessment, and storage location.\n* Deviations and corrective actions.\n\n---\n\n**End of SOP**"
                }
            ]
        },
        {
            "@id": "#protocol-37",
            "@type": "LabProtocol",
            "name": "PCR-cDNA Barcoding sequencing library preparation (SQK-PCB111.24)",
            "description": "Oxford Nanopore Technologies PCR-cDNA Barcoding kit (SQK-PCS111) for full-length cDNA library preparation from total RNA. Uses template-switching reverse transcription followed by PCR amplification with barcoded primers. Compatible with MinION, GridION and PromethION flow cells (R9.4.1). Superseded by SQK-PCS114 in late 2023.",
            "url": {
                "@id": "https://community.nanoporetech.com/docs/prepare/library_prep_protocols/pcr-cdna-barcoding-kit-sqk-pcb111-24"
            },
            "version": "1.0",
            "category": "sample_prep",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "## Overview & Kit Contents\n\nThis protocol is based on the Oxford Nanopore Technologies **PCR-cDNA Barcoding Kit (SQK-PCB111.24)**, suitable for generating full-length barcoded cDNA sequencing libraries from total RNA.\n\n### Kit components\n- VNP primer, SSP primer (strand-switching primer)\n- Barcoded PCR primers (BC01\u2013BC24)\n- Rapid Adapter (RAP)\n- Elution Buffer (EB)\n\n### Required reagents (user-supplied)\n- Lambda Exonuclease\n- NEBNext Quick Ligation Reaction Buffer\n- T4 DNA Ligase 2M U/ml\n- 10 mM dNTP solution\n- LongAmp Hot Start Taq 2X Master Mix\n- Maxima H Minus Reverse Transcriptase (200 U/ul) with 5x RT Buffer\n- RNaseOUT, 40 U/ul\n- USER (Uracil-Specific Excision Reagent) Enzyme\n- Exonuclease I\n- AMPure XP beads\n\n### Required equipment\n- Thermal cycler, magnetic rack, Qubit fluorometer, vortex mixer, microcentrifuge"
                },
                {
                    "@type": "HowToStep",
                    "position": 2,
                    "text": "### Input RNA requirements\n\n- **Input**: 1\u201350 ng poly(A)+ mRNA or 50\u2013100 ng total RNA (RIN >= 7 recommended)\n- RNA should be DNase-treated if genomic DNA contamination is suspected\n- Prepare RNA in 7.5 uL nuclease-free water\n- Add 1 uL VNP primer (2 uM) and 1 uL 10 mM dNTPs\n- Incubate at **65 C for 5 min**, then snap-cool on ice for 1 min"
                },
                {
                    "@type": "HowToStep",
                    "position": 3,
                    "text": "### Reverse transcription and strand switching (~90 min)\n\n1. Prepare RT mix on ice (per reaction):\n   - 4 uL 5x RT Buffer\n   - 1 uL RNaseOUT (40 U/uL)\n   - 1 uL nuclease-free water\n   - 2 uL Strand-Switching Primer (SSP, 10 uM)\n   - 1 uL Maxima H Minus RT (200 U/uL)\n2. Add 10 uL RT mix to the 9.5 uL RNA/primer/dNTP mix (total 19.5 uL)\n3. Incubate: **42 C for 90 min** (RT + template switching), **85 C for 5 min** (inactivation), hold 4 C\n4. Add 1 uL RNase H, incubate **37 C for 10 min**\n5. Briefly centrifuge and place on ice"
                },
                {
                    "@type": "HowToStep",
                    "position": 4,
                    "text": "### PCR amplification with barcoded primers (~30 min)\n\n1. Prepare PCR reaction (50 uL total):\n   - 20 uL cDNA from RT step\n   - 25 uL LongAmp Taq 2X Master Mix\n   - 1.5 uL Barcode Primer (BC*XX*, 10 uM)\n   - 3.5 uL nuclease-free water\n2. Cycling conditions:\n   - **95 C, 3 min** (initial denaturation)\n   - **18 cycles**: 95 C 15 s / 62 C 15 s / 65 C 5 min\n   - **65 C, 6 min** (final extension)\n   - Hold at 4 C\n\n**Note**: 18 cycles is suitable for 50-100 ng total RNA input. Reduce to 14-15 cycles for higher inputs."
                },
                {
                    "@type": "HowToStep",
                    "position": 5,
                    "text": "### Bead clean-up and quantification\n\n1. Add 40 uL AMPure XP beads (0.8x ratio) to 50 uL PCR product\n2. Incubate at RT for 5 min\n3. Place on magnetic rack, wait for pellet to form\n4. Wash 2x with 200 uL freshly prepared 80% ethanol\n5. Air-dry pellet for ~30 s (do not over-dry)\n6. Elute in 12 uL EB\n7. Quantify with Qubit dsDNA HS assay\n8. Expected yield: 50-500 ng depending on input RNA quality and quantity"
                },
                {
                    "@type": "HowToStep",
                    "position": 6,
                    "text": "### Pool barcoded libraries and attach Rapid Adapter\n\n1. Pool equimolar amounts of barcoded cDNA libraries (target: 100-200 fmol total)\n2. Adjust volume to 11 uL with EB\n3. Add 1 uL Rapid Adapter (RAP)\n4. Mix gently by flicking, spin down briefly\n5. Incubate at **RT for 5 min**\n6. Library is ready for loading -- **do not vortex** after adapter attachment"
                },
                {
                    "@type": "HowToStep",
                    "position": 7,
                    "text": "### Flow cell priming and loading\n\n1. Prepare priming mix: 30 uL FCT + 1170 uL FCF\n2. Load 800 uL priming mix via priming port, wait 5 min\n3. Prepare loading mix: 12 uL adapted library + 37.5 uL SB + 25.5 uL LB\n4. Load remaining priming mix, then load 75 uL library via SpotON port\n5. Start sequencing in MinKNOW (Kit: SQK-PCB111.24, enable barcode demultiplexing)"
                },
                {
                    "@type": "HowToStep",
                    "position": 8,
                    "text": "## References\n\n- Oxford Nanopore Technologies, **\"PCR-cDNA Barcoding Kit (SQK-PCB111.24)\"**.\n- Earlier version: SQK-PCS111. Kit superseded by SQK-PCS114/SQK-PCB114 (V14 chemistry, R10.4.1 flow cells) in late 2023."
                }
            ]
        },
        {
            "@id": "#protocol-44",
            "@type": "LabProtocol",
            "name": "Fragment Analyzer \u2013 SS NGS Fragment 1-6000 bp",
            "description": "Standard sensitivity NGS fragment analysis (1-6000 bp range) on the Fragment Analyzer using the DNF-473 kit. Suitable for QC of NGS libraries, PCR products, and size-selected DNA fragments in the 1-6000 bp range.",
            "url": {
                "@id": "https://www.agilent.com/store/en_US/Prod-DNF-473-0500/DNF-473-0500"
            },
            "version": "1.0",
            "category": "measurement",
            "step": [
                {
                    "@type": "HowToStep",
                    "position": 1,
                    "text": "**Method:** `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds`\n\n**Instrument:** Agilent/Advanced Analytical Fragment Analyzer (33 cm capillary array)\n\n**Analysis Mode:** NGS\n\n---\n\n## 1. Document Control\n\n* **SOP ID:** FA-NGS-1-6000bp-33cm\n* **Version:** 1.0\n* **Effective Date:** __________\n* **Prepared by:** __________\n* **Approved by:** __________\n* **Review cycle:** 12 months\n\n## 2. Purpose\n\nTo define the procedure for running standard-sensitivity NGS fragment analysis on the Fragment Analyzer using the **DNF-473-33** method. This method resolves DNA fragments in the **1\u20136,000 bp** range with standard sensitivity, suitable for:\n\n* QC of NGS libraries (amplicon, PCR-cDNA, shotgun)\n* Assessment of size selection efficiency (e.g. AMPure bead selection)\n* Verification of library size distribution prior to sequencing\n* Quantification of library concentration\n\n## 3. Scope\n\nApplies to all operators performing NGS library QC or fragment analysis in the 1\u20136,000 bp range using the DNF-473 kit on a Fragment Analyzer.\n\n## 4. Responsibilities\n\n* **Operator:** Execute this SOP, record run metadata, verify QC, and report issues.\n* **Lab lead:** Ensure training, instrument maintenance, and SOP review.\n\n## 5. Safety\n\n* Wear lab coat, safety glasses, and disposable gloves.\n* Handle gels, buffers, and plates per SDS.\n* Avoid contact with high voltage areas during operation. Only open instrument when software indicates safe.\n* Dispose of consumables as per local regulations.\n\n## 6. References\n\n* Fragment Analyzer user manual.\n* DNF-473 SS NGS Fragment 1-6000bp kit instructions.\n* Local laboratory policies for data retention and equipment maintenance.\n\n## 7. Materials and Equipment\n\n**Instrument and configuration**\n\n* Fragment Analyzer with **33 cm** capillary array.\n* Array serial and usage count recorded before each run.\n\n**Reagents and consumables**\n\n* SS NGS Fragment 1-6000bp gel cartridge compatible with `DNF-473-33`.\n* Buffer reservoirs and rinse solution as per kit.\n* 96-well plate(s) and plate seals or caps.\n* DNA ladder (supplied with kit or compatible sizing standard).\n* Nuclease-free water and low-retention tips.\n\n**Software**\n\n* ProSize 3.0 or later for data analysis and PDF report generation.\n\n## 8. Sample Requirements\n\n* DNA libraries in TE, EB, or nuclease-free water.\n* Recommended input: **0.2\u201350 ng/\u00b5L** (standard sensitivity range).\n* Minimum volume per well: as per plate format (typically 2 \u00b5L sample + diluent).\n* Avoid EDTA >5 mM, high salt, or detergents that interfere with electrokinetic injection.\n* For very low concentration libraries (<1 ng/\u00b5L, e.g. post-bead-selection), results may show concentration but no resolved peaks \u2014 this is expected and the TIM value is still informative.\n\n## 9. Pre-Run Checks\n\n1. Inspect capillary array for leaks, kinks, or salt crystallisation. Record **array serial** and **usage count**.\n2. Confirm instrument details in software:\n   * **Device Serial:** record\n   * **FA Version:** record\n3. Verify consumables:\n   * Correct **gel** loaded and **Gel Selection: Gel 1**.\n   * Fresh buffer and rinse trays in correct positions.\n4. Equilibrate reagents to room temperature if required by kit.\n5. Prepare plate map with samples, ladder, controls, and blanks.\n\n## 10. Plate Preparation\n\n1. Thaw and mix reagents gently. Spin down briefly.\n2. Aliquot ladder into designated well (typically position 12 of each row).\n3. Aliquot samples into designated wells per plate map.\n4. Include at least one blank (nuclease-free water) for baseline reference.\n5. Seal the plate. Briefly centrifuge to remove bubbles. Keep sealed until loading.\n\n## 11. Instrument Setup (Software)\n\nSet parameters as follows:\n\n| Parameter | Setting |\n|---|---|\n| Method Name | `DNF-473-33 - SS NGS Fragment 1-6000bp.mthds` |\n| Analysis Mode | `NGS` |\n| Gel Prime | `No` |\n| Full Conditioning | `Yes` (start of day) |\n| Gel Selection | `Gel 1` |\n| Perform Prerun | `5.0 kV` for `30 s` |\n| Rinse | Tray `3`, Row `A`, `# Dips: 2` |\n| Marker 1 | `No` |\n| Sample Injection | `5.0 kV` for `4 s` |\n| Separation | `8.0 kV` for `40.0 min` |\n| # of Capillaries | Per array configuration |\n\n**Note:** The DNF-473 method uses **shorter separation time (40 min)** and **higher separation voltage (8 kV)** compared to the HS Large Fragment method (DNF-464: 60 min, 5 kV), reflecting the smaller target size range.\n\n## 12. Run Procedure\n\n1. Load the sealed sample plate into the instrument.\n2. Confirm tray positions and liquid levels.\n3. In software, confirm the method parameters match Section 11.\n4. Start the run. Observe for the first 2\u20133 minutes for abnormal current, leaks, or errors.\n5. Allow the **40 min** separation to complete.\n\n## 13. Data Analysis (ProSize)\n\n1. Open the completed run in ProSize 3.0.\n2. Verify ladder sizing is correct (expected peaks at kit-specified positions).\n3. For each sample, review:\n   * **Electropherogram** \u2014 peak shape, baseline, and resolution\n   * **Total Concentration (ng/\u00b5L)** \u2014 integrated from all non-marker peaks\n   * **TIM (nmol/L)** \u2014 total integrated molarity\n   * **Main peak size (bp)** \u2014 dominant fragment size\n   * **Number of peaks** \u2014 indicator of library complexity/purity\n4. Export PDF report containing per-sample peak tables, electropherograms, and summary metrics.\n\n## 14. Interpretation Guide\n\n| Observation | Interpretation | Action |\n|---|---|---|\n| Single clean peak at expected size | Library prep and/or size selection successful | Proceed to pooling/sequencing |\n| Broad peak or multiple peaks | Heterogeneous library or incomplete size selection | Consider re-selection or accept depending on application |\n| No peaks but measurable concentration | Library below detection threshold for sizing | Verify by Qubit; may still be usable if concentration is sufficient |\n| Adapter dimer peak (~100-150 bp) | Incomplete cleanup | Re-clean with AMPure beads at appropriate ratio |\n| No signal | Failed library prep, sample degraded, or loading error | Troubleshoot: check sample, re-run, or re-prep |\n\n### Typical Results for ONT PCR-cDNA Libraries\n\n| Library state | Expected concentration | Expected main peak |\n|---|---|---|\n| Pre-size-selection | 4\u201310 ng/\u00b5L | 1,000\u20132,000 bp (broad) |\n| Post-bead-selection (1.5 kb) | 0.5\u20132 ng/\u00b5L | ~1,480 bp (single peak) |\n| Post-bead-selection (4 kb) | 0.5\u20132 ng/\u00b5L | ~3,500\u20134,500 bp (single peak) |\n\n## 15. Quality Control and Acceptance\n\n* Ladder wells exhibit expected profile and sizing.\n* Baseline is stable with distinct peaks where applicable.\n* Migration time consistent across capillaries.\n* If any criterion fails, document, recondition, and repeat controls before analysing unknowns.\n\n## 16. Troubleshooting\n\n| Problem | Action |\n|---|---|\n| Weak or absent signal | Verify injection settings, sample concentration, plate seal integrity, capillary tips in wells |\n| High noise or drifting baseline | Re-condition array, check buffer freshness and tray cleanliness |\n| Inconsistent sizing | Confirm ladder integrity, plate map accuracy, and temperature stability |\n| Frequent current faults | Inspect for bubbles, low liquid levels, or salt contamination; replace consumables |\n\n## 17. Maintenance\n\n* Perform **Full Conditioning** at the start of the day or as required by kit.\n* Replace gels and buffers per shelf-life and use counts.\n* Track **Array Usage Count** at each run and follow vendor guidance for replacement.\n* Clean trays weekly or after spills. Inspect electrodes and stage area.\n\n## 18. Records to Retain\n\nFor each run record:\n\n* Date, time, and operator\n* Device serial and FA software version\n* Array serial and usage count\n* Method name and key parameters\n* Plate map and sample IDs\n* File path to raw data and PDF reports\n* QC outcomes and any deviations\n\n---\n\n**End of SOP**"
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}